Gene Dmel\bnl

(Sutherland et al., 1995)

92B-92B

(determined by in situ hybridisation)

92B-92B

(determined by in situ hybridisation) 92B2--3 (determined by in situ hybridisation)

(BDGP Project Members, 1994-1999)

92B-92B

(determined by in situ hybridisation)

(Cooley et al., 1988)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\bnl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

bnl-RA

FBtr0083795

NM_169874

3632

770

bnl-RC

FBtr0300123

NM_001144611

4295

760

Additional Transcript Data & Comments

Reported size (kB)

4 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

bnl-PA

FBpp0083206

84.1

770

9.97

NP_732452

AAF55701

bnl-PC

FBpp0289400

82.9

760

9.97

NP_001138083

ACL83539

Additional Polypeptide Data & Comments

Reported size (kDa)

770 (aa); 84 (kD predicted)

Comments

External Data

FBsf0000436214
3R:15,625,107..15,625,880

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Heparin-binding growth factor/Fibroblast growth factor (IPR002209)

Interleukin 1/heparin-binding growth factor (IPR002348)

Cytokine, IL-1-like (IPR008996)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

regulatory region

evidence=Reporter construct (in vivo)

(Junion et al., 2012, Halfon, 2012.6.19)

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

dorsal ectoderm anlage

Comment:anlage in statu nascendi

embryonic stage 7 -- 8

dorsal ectoderm

head mesoderm

embryonic stage 9 -- 10

dorsal ectoderm

head mesoderm

embryonic stage 11 -- 12

anterior spiracle primordium

Comment:reported as anterior spiracle specific anlage

dorsal epidermis primordium

salivary gland body primordium

tracheal primordium

(Sato and Kornberg, 2002)

third instar larval stage

wing disc | anterior/posterior compartment boundary & dorsal & restricted & vicinity of

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

posterior transverse furrow

cephalic furrow

embryonic stage 11 -- 17

tracheal air sac | surrounding

Additional Descriptive Data

bnl is expressed in embyros with a peak at 5-11hr and is not expressed at later stages. By in situ hybridization, bnl expression is first observed around the cephalic furrow and the posterior transverse furrow. Starting in stage 11, bnl transcripts are expressed dynamically in clusters outside the tracheal system at every position where a major tracheal branch will bud.

(Sato and Kornberg, 2002)

In the wing disc, transcript was detected in between 15 and 60 and 80 and 150 columnar epithelial cells in early and late third instar larvae, respectively. The cells straddle the anterior/posterior compartment border and are dosal to the prospective wing blade.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(The modENCODE Consortium, 2010, Graveley et al., 2011)

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression in all larval and adult organs/tissues ranges from low to undetected. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult hindgut, adult Malpighian tubules, adult salivary gland, larval trachea.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		3
central nervous system, larvae L3		1
central nervous system, pupae P8		7
head, virgin 1-day female		4
head, virgin 4-day female		3
head, virgin 20-day female		2
head, mated 1-day female		3
head, mated 4-day female		2
head, mated 20-day female		2
head, mated 1-day male		5
head, mated 4-day male		4
head, mated 20-day male		5
salivary gland, larvae L3 wandering		0
salivary gland, white prepupae		0
digestive system, larvae L3 wandering		3
digestive system, 1-day adult		1
digestive system, 4-day adult		2
digestive system, 20-day adult		3
fat body, larvae L3 wandering		1
fat body, white prepupae		1
fat body, pupae P8		2
carcass, larvae L3 wandering		4
carcass, 1-day adult		3
carcass, 4-day adult		4
carcass, 20-day adult		3
ovary, virgin 4-day female		0
ovary, mated 4-day female		0
testis, mated 4-day male		1
accessory gland, mated 4-day male		0
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 00-18 hour embryonic stages, during late pupal stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		0
embryo 02-04hr		3
embryo 04-06hr		10
embryo 06-08hr		12
embryo 08-10hr		11
embryo 10-12hr		6
embryo 12-14hr		7
embryo 14-16hr		5
embryo 16-18hr		5
embryo 18-20hr		5
embryo 20-22hr		3
embryo 22-24hr		3
larva L1		3
larva L2		4
larva L3 12hr old		3
larva L3 puffstage 1-2		2
larva L3 puffstage 3-6		2
larva L3 puffstage 7-9		3
white prepupae new		2
white prepupae 12hr		3
white prepupae 24hr		3
pupae 2d postWPP		7
pupae 3d postWPP		7
pupae 4d postWPP		6
adult male 01day		3
adult male 05day		3
adult male 30day		3
adult female 01day		2
adult female 05day		1
adult female 30day		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		35
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		57
ovary OSC		81
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		(35)
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		(57)
ovary OSC		(81)
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		35
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		57
ovary OSC		81
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		35
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		57
ovary OSC		81
ovary OSS		12
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		35
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		57
ovary OSC		81
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		(35)
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		(57)
ovary OSC		(81)
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		35
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		57
ovary OSC		81
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		13
Schneider line 2 Sg4		2
embryonic 1182-4H		1
embryonic GM2		8
embryonic Kc167		35
embryonic S1		9
embryonic S3		11
leg disc CME L1		5
wing disc CME-W2		12
wing disc ML-DmD8		4
wing disc ML-DmD9		4
wing disc ML-DmD16-c3		2
wing disc ML-DmD21		2
wing disc ML-DmD32		2
haltere disc ML-DmD17-c3		3
eye-antennal disc ML-DmD11		9
antennal disc ML-DmD20-c5		1
mixed discs ML-DmD4-c1		0
CNS ML-DmBG1-c1		5
CNS ML-DmBG2-c2		4
tumorous blood cells mbn2		11
ovary fGS/OSS		57
ovary OSC		81
ovary OSS		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		1
cold shock, 4-day adult		1
heat shock, 4-day adult		2
Cadmium 50 mM 6 hrs, larvae L3		1
Cadmium 50 mM 12 hrs, larvae L3		2
Cadmium 50 mM 48 hrs, 4-day adult		2
Cadmium 100 mM 48 hrs, 4-day adult		3
Copper 0.5 mM 12 hrs, larvae L3		2
Copper 15 mM 48 hrs, 4-day adult		4
Zinc 5 mM 12 hrs, larvae L3		1
Zinc 4.5 mM 48 hrs, 4-day adult		3
Ethanol 2.5% 3 hrs, larvae L3		2
Ethanol 5% 3 hrs, larvae L3		2
Ethanol 10% 3 hrs, larvae L3		1
Caffeine 1.5 mg/ml 4 hrs, larvae L3		2
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1
Caffeine 25 mg/ml 48 hrs, 4-day adult		1
Paraquat 5 mM 48 hrs, 4-day adult		2
Paraquat 10 mM 48 hrs, 4-day adult		5
Rotenone 2 μg 12 hrs, larvae L3		2
Rotenone 8 μg 12 hrs, larvae L3		1
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

Show all FlyExpress processed images for bnl

BDGP expression data

•

GH08887

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0014135

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4608-RB;CG4608-RA;CG4608-RA

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

viable, with Scer\GAL4^pnr-MD237

bnl^GD3070

Other Phenotypes

Allele

cell adhesion defective | somatic clone | third instar larval stage

bnl⁰⁰⁸⁵⁷

cell migration defective | second instar larval stage | somatic clone

bnl⁰⁰⁸⁵⁷

cell migration defective | somatic clone | third instar larval stage

bnl⁰⁰⁸⁵⁷

hyperplasia | third instar larval stage | heat sensitive

bnl^hs.PS

visible | adult stage | somatic clone

Phenotype manifest in

Allele

actin cytoskeleton | pupal stage | somatic clone

bnl^JR69

air sac tracheoblast of dorsal air sac primordium, with Scer\GAL4^Act5C.PI

bnl^Scer\UAS.cSa

air sac tracheoblast of dorsal air sac primordium | cell non-autonomous | somatic clone

bnl⁰⁰⁸⁵⁷

air sac tracheoblast of dorsal mesothoracic disc & filopodium

bnl^hs.PS

air sac tracheoblast of dorsal mesothoracic disc & filopodium | somatic clone | cell non-autonomous

bnl^αTub84B.PS

dorsal air sac primordium | third instar larval stage | heat sensitive

bnl^hs.PS

embryonic/larval dorsal branch, with Scer\GAL4^btl.PS

bnl^Scer\UAS.cSa

embryonic/larval dorsal trunk

bnl⁰⁰⁸⁵⁷

embryonic/larval dorsal trunk, with Scer\GAL4^btl.PS

bnl^Scer\UAS.cSa

embryonic/larval ganglionic branch

embryonic/larval ganglionic branch, with Scer\GAL4^A9

bnl^Scer\UAS.cSa

embryonic/larval ganglionic branch, with Scer\GAL4^sim.PS

bnl^Scer\UAS.cSa

embryonic/larval lateral trunk, with Scer\GAL4^btl.PS

bnl^Scer\UAS.cSa

embryonic/larval trachea

bnl⁰⁰⁸⁵⁷

embryonic/larval trachea, with Scer\GAL4^71B

bnl^Scer\UAS.cSa

embryonic/larval trachea, with Scer\GAL4^A9

bnl^Scer\UAS.cSa

embryonic/larval trachea, with Scer\GAL4^btl.PS

bnl^Scer\UAS.cSa

embryonic/larval trachea, with Scer\GAL4^hs.PB

bnl^Scer\UAS.cSa

embryonic/larval trachea, with Scer\GAL4^tey-5053A

bnl^Scer\UAS.cSa

embryonic/larval trachea | somatic clone, with Scer\GAL4^Act5C.PP

bnl^Scer\UAS.cSa

embryonic/larval tracheal system

embryonic/larval tracheal system, with Scer\GAL4^69B

bnl^Scer\UAS.cSa

embryonic/larval tracheal system, with Scer\GAL4^C49

bnl^Scer\UAS.cSa

embryonic/larval tracheal system, with Scer\GAL4^hs.PB

bnl^Scer\UAS.cSa

embryonic/larval tracheal system, with Scer\GAL4^ptc-559.1

bnl^{Scer\UAS.P\T.cRa}

embryonic tracheal system & filopodium | ectopic, with Scer\GAL4^btl.PS

bnl^Scer\UAS.cSa

eye | somatic clone

ganglionic branch primordium

lateral G branch

bnl⁰⁰⁸⁵⁷

lateral G branch, with Scer\GAL4^hs.PB

bnl^Scer\UAS.cSa

lateral G branch, with Scer\GAL4^tey-5053A

bnl^Scer\UAS.cSa

lateral H branch

bnl⁰⁰⁸⁵⁷

ommatidium | somatic clone | third instar larval stage

bnl^JR69

presumptive embryonic/larval tracheal system

bnl⁰⁰⁸⁵⁷

presumptive embryonic/larval tracheal system, with Scer\GAL4^btl.PS

bnl^Scer\UAS.cSa

subretinal glial cell | second instar larval stage | somatic clone

bnl⁰⁰⁸⁵⁷

subretinal glial cell | somatic clone | third instar larval stage

bnl⁰⁰⁸⁵⁷

trachea, with Scer\GAL4^sim.PS

bnl^Scer\UAS.cSa

tracheal metamere 1 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 2 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 3 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 4 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 5 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 6 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 7 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 8 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 9 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal metamere 10 | embryonic stage

bnl⁰⁰⁸⁵⁷

tracheal system

bnl⁷⁰⁸

tracheal tip cell | embryonic stage, with Scer\GAL4^hs.PB

bnl^Scer\UAS.cSa

visceral branch, with Scer\GAL4^bap.3

bnl^Scer\UAS.cSa

Classical Alleles ( 16 )

For All Classical Alleles Show

Allele of bnl	Class	Mutagen	Stocks	Known lesion
bnl^KG00157		P-element activity	2	--
bnl⁰⁰⁸⁵⁷	amorphic allele - genetic evidence, loss of function allele	P-element activity	1	Yes
bnl⁰⁶⁹¹⁶	hypomorphic allele - genetic evidence	P-element activity	1	Yes
bnl^CB-6103-3			1	--
bnl^e03115		piggyBac transposase	1	--
bnl^f01868		piggyBac transposase	1	--
bnl^MI00874			1	--
bnl^MI01635			1	--
bnl^neo52		P-element activity	1	--
bnl⁷⁰⁸		ethyl methanesulfonate	0	--
bnl^c01939		piggyBac transposase	0	--
bnl^e00029		piggyBac transposase	0	--
bnl^JR69			0	--
bnl^PL00790		piggyBac transposase	0	Yes
bnl^S044026		P-element activity	0	--
bnl^unspecified			0	--

Alleles Carried on Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Allele of bnl	Mutagen	Stocks	Known lesion
bnl^GD3070	in vitro construct - RNAi in vitro construct - regulatory fusion	2	Yes
bnl^HMS01046	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
bnl^KK109317	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
bnl^hs.PS	in vitro construct - regulatory fusion	0	Yes
bnl^NIG.4608R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
bnl^{Scer\FRT.Disc\RFP-DsRed2.y.αTub84B}	in vitro construct - regulatory fusion in vitro construct	0	Yes
bnl^Scer\UAS.cSa	in vitro construct - regulatory fusion	0	--
bnl^{Scer\UAS.P\T.cRa}	in vitro construct - regulatory fusion	0	Yes
bnl^αTub84B.PS	FLPase	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3R)BSC636

(Christensen et al., 2008.9.29)

Df(3R)BSC808

(Christensen et al., 2009.5.6)

Df(3R)BSC809

(Christensen et al., 2009.5.6)

Df(3R)Dl-BX12

(Spradling et al., 1999, Jarecki et al., 1999, BDGP Project Members, 1994-1999, Sutherland et al., 1996)

Df(3R)ED6025

(Ryder and Roote, 2004.11.10)

Not disrupted in

Df(3R)H-B79

(Spradling et al., 1999)

Df(3R)p712

(BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

heat-shock construct

UAS construct

characterization construct

P{αTub84B(FRT.Disc\RFP^DsRed2.y⁺)bnl}

P{αTub84B(-FRT)bnl}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}bnl^S044026

P{PZ}bnl⁰⁰⁸⁵⁷

P{PZ}bnl⁰⁶⁹¹⁶

miscellaneous insertions

P{SUPor-P}bnl^KG00157

P{UASp-YFP.Rab39.Q69L}14

P{UASp-YFP.RabX1.Q66L}02

P{UAST-YFP.Rab6.T26N}03

insertion of enhancer trap binary system

PBac{GAL4D,EYFP}bnl^PL00790

insertion of mobile activating element

P{GSV1}C904.1^C904.1

P{GSV1}GSd325^GSd325

Gene Ontology: Function, Process & Cellular Component ( 19 unique terms )

Terms Based on Experimental Evidence ( 7 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

activation of MAPK activity

References

inferred from expression pattern

(Ohshiro et al., 2002)

cytoneme assembly

inferred from direct assay

(Ramirez-Weber and Kornberg, 1999)

filopodium assembly

inferred from mutant phenotype

(Ribeiro et al., 2002)

genital disc development

inferred from expression pattern

(Ahmad and Baker, 2002)

glial cell migration

inferred from mutant phenotype

(Mukherjee et al., 2012)

open tracheal system development

inferred from mutant phenotype

(Jarvis et al., 2006)

positive chemotaxis

inferred from direct assay

(Sato and Kornberg, 2002)

Cellular Component ( 0 terms)

Terms Based on Predictions or Assertions ( 14 terms )

Molecular Function

CV term

fibroblast growth factor receptor binding

References

inferred from sequence or structural similarity with mouse Fgf10

(FlyBase, 1992-)

non-traceable author statement

(Sutherland, 1996.12.15)

traceable author statement

(Affolter, 2000, Wilson and Leptin, 2000, Ghabrial et al., 2003, Itoh and Ornitz, 2004)

growth factor activity

inferred from electronic annotation with InterPro:IPR002209, InterPro:IPR002348

(FlyBase Curators et al., 2004-)

Biological Process

CV term

(Wilson and Leptin, 2000)

References

chemotaxis

traceable author statement

epithelial cell migration, open tracheal system

traceable author statement

(Zelzer and Shilo, 2000, Affolter, 2000, Rosin and Shilo, 2002, Uv et al., 2003, Nelson, 2003)

fibroblast growth factor receptor signaling pathway

non-traceable author statement

(Rosin and Shilo, 2002)

traceable author statement

(Affolter, 2000, Ghabrial et al., 2003, Itoh and Ornitz, 2004)

morphogenesis of a branching structure

traceable author statement

(Affolter and Shilo, 2000)

open tracheal system development

non-traceable author statement

(Wilson and Leptin, 2000)

traceable author statement

(Ribeiro et al., 2003)

positive chemotaxis

traceable author statement

(Affolter, 2000, Ribeiro et al., 2003, Ghabrial et al., 2003)

primary branching, open tracheal system

traceable author statement

(Uv et al., 2003, Ghabrial et al., 2003)

regulation of tube architecture, open tracheal system

traceable author statement

(Uv et al., 2003)

secondary branching, open tracheal system

traceable author statement

(Ghabrial et al., 2003)

terminal branching, open tracheal system

traceable author statement

(Ghabrial et al., 2003)

tracheal outgrowth, open tracheal system

traceable author statement

(Ghabrial et al., 2003)

Cellular Component

CV term

(Sutherland et al., 1996, FlyBase, 1992-)

References

extracellular region

inferred from sequence or structural similarity

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

bnl allele

Gene

References

bnl⁰⁰⁸⁵⁷

btl

btl::tor

grh

(Hemphala et al., 2003)

stumps

(Imam et al., 1999)

bnl^Scer\UAS.cSa

(Wolf and Schuh, 2000)

(Dorfman et al., 2002)

stumps

(Imam et al., 1999)

unspecified

csw

(Jarvis et al., 2006)

stumps

(Michelson et al., 1998)

trimmed

(Shim et al., 1998)

External Data

y¹ sc^* v¹; P{TRiP.HMS01046}attP2

BioGRID - A database of protein and genetic interactions

•

67346

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0014135

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

42356

SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores.

•

Q9VDT9

Orthologs

OrthoDB Orthologs (26) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6WH95R

Dipteran inclusive ortholog search

EOG6RBQT6

Insect inclusive ortholog search

EOG6B8H9K

Arthropod inclusive ortholog search

EOG65HQDS

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG6WH95R)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18297

Drosophila persimilis

Dper\GL11947

Drosophila willistoni

Dwil\GK22814

Drosophila mojavensis

Dmoj\GI10332

Drosophila grimshawi

Dgri\GH23362

Orthologs in non-Drosophila Dipterans (EOG6RBQT6)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Anopheles gambiae

Malaria mosquito

Agam\AGAP004181

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ019826

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001329

Orthologs in non-Dipteran Insects (EOG6B8H9K)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB45927

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18742

Atta cephalotes

Leafcutter ant

Acep\ACEP12046

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO13441

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL18351

Linepithema humile

Argentine ant

Lhum\LH20340

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB15936

Solenopsis invicta

Red fire ant

Sinv\SINV21550

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI45753

Pediculus humanus

Human body louse

Phum\PHUM491400

Tribolium castaneum

Red flour beetle

Tcas\TC001760

Orthologs in non-Insect Arthropods (EOG65HQDS)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_108237

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18297

Drosophila persimilis

Dper\GL11947

Drosophila willistoni

Dwil\GK22814

Drosophila virilis

Dvir\GJ10186

Drosophila mojavensis

Dmoj\GI10332

Drosophila grimshawi

Dgri\GH23362

Stocks & Reagents

Stocks Listed in FlyBase ( 14 )

Bloomington

6384

ry⁵⁰⁶ P{PZ}bnl⁰⁰⁸⁵⁷/TM3, Sb¹

34572

11704

ry⁵⁰⁶ P{PZ}bnl⁰⁶⁹¹⁶/TM3, ry^RK Sb¹ Ser¹

34228

y¹ w^*; Mi{MIC}bnl^MI01635/TM3, Sb¹ Ser¹

34745

y¹ w^*; Mi{MIC}bnl^MI00874/TM3, Sb¹ Ser¹

13052

y¹ w^67c23; ry⁵⁰⁶ P{SUPor-P}bnl^KG00157/TM3, Sb¹ Ser¹

123916

w¹¹¹⁸; P{RS3}bnl^CB-6103-3

VDRC

v101377

P{KK109317}VIE-260B

v5732

w¹¹¹⁸ P{GD3070}v5732

v5730

w¹¹¹⁸; P{GD3070}v5730

More stocks available...

Genomic Clones ( 2 )

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 15 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

GH08887

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

LP07909

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0014135

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

42356

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: bnl CG4608

(Gong, 1999.11)

Source for database merge of

Additional comments

Other Comments

Haploinsufficient locus (not associated with strong haplolethality or haplosterility).

(Cook et al., 2012)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

bnl expressed in each trunk visceral mesodermal parasegment serves as a guide for the initial budding of tracheal visceral branches.

(Hosono et al., 2003)

sr acts co-operatively with bnl to facilitate extension of tracheal branches.

(Dorfman et al., 2002)

Oxygen deprivation stimulates expression of bnl.

(Jarecki et al., 1999)

bnl acts as a chemoattractant that guides the terminal branches of trachea during embryogenesis.

(Jarecki et al., 1999)

dpp plays a dual role during tracheal cell migration. dpp controls the region-specific activation of bnl in the dorsal part of the embryo. dpp expression dorsal and ventral to the tracheal placode at the onset of migration instructs groups of tracheal cells with respect to their migration behaviour. Results suggest that other factors in addition to bnl dictate the direction of migration along the dorsoventral axis: some of these factors might be recognised by tracheal cells only upon the reception of the dpp signal.

(Vincent et al., 1997)

bnl locus is haploinsufficient.

bnl is a key determinant of the tracheal branching pattern. bnl is required for tracheal branching and is expressed dynamically in clusters of cells surrounding the developing tracheal system at each position where a new branch will form and grow out. Misexpression of bnl activates later programs of tracheal gene expression and branching, resulting in massive networks of branches. bnl appears to function as a ligand for btl.