FGF, l(3)06916, fibroblast growth factor
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.54
4 (northern blot)
770 (aa); 84 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\bnl using the Feature Mapper tool.
In the wing disc, transcript was detected in between 15 and 60 and 80 and 150 columnar epithelial cells in early and late third instar larvae, respectively. The cells straddle the anterior/posterior compartment border and are dosal to the prospective wing blade.
bnl is expressed in embyros with a peak at 5-11hr and is not expressed at later stages. By in situ hybridization, bnl expression is first observed around the cephalic furrow and the posterior transverse furrow. Starting in stage 11, bnl transcripts are expressed dynamically in clusters outside the tracheal system at every position where a major tracheal branch will bud.
GBrowse - Visual display of RNA-Seq signalsView Dmel\bnl in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: bnl CG4608
Haploinsufficient locus (not associated with strong haplolethality or haplosterility).
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
bnl expressed in each trunk visceral mesodermal parasegment serves as a guide for the initial budding of tracheal visceral branches.
bnl acts as a chemoattractant that guides the terminal branches of trachea during embryogenesis.
dpp plays a dual role during tracheal cell migration. dpp controls the region-specific activation of bnl in the dorsal part of the embryo. dpp expression dorsal and ventral to the tracheal placode at the onset of migration instructs groups of tracheal cells with respect to their migration behaviour. Results suggest that other factors in addition to bnl dictate the direction of migration along the dorsoventral axis: some of these factors might be recognised by tracheal cells only upon the reception of the dpp signal.
bnl is a key determinant of the tracheal branching pattern. bnl is required for tracheal branching and is expressed dynamically in clusters of cells surrounding the developing tracheal system at each position where a new branch will form and grow out. Misexpression of bnl activates later programs of tracheal gene expression and branching, resulting in massive networks of branches. bnl appears to function as a ligand for btl.