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General Information
Symbol
Dmel\Oda
Species
D. melanogaster
Name
Ornithine decarboxylase antizyme
Annotation Symbol
CG16747
Feature Type
FlyBase ID
FBgn0014184
Gene Model Status
Stock Availability
Gene Snapshot
Also Known As

guf, gut feeling, AZ, gutfeeling

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:12,166,295..12,174,264 [-]
Recombination map

2-66

RefSeq locus
NT_033778 REGION:12166295..12174264
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (6 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001616256
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:8095
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001616256
(assigned by GO_Central )
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001616256
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001616256
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the ODC antizyme family. (P54361)
Summaries
Protein Function (UniProtKB)
Ornithine decarboxylase (ODC) antizyme protein that negatively regulates ODC activity and intracellular polyamine biosynthesis and uptake in response to increased intracellular polyamine levels. Binds to ODC monomers, inhibiting the assembly of the functional ODC homodimer, and targets the monomers for ubiquitin-independent proteolytic destruction by the 26S proteasome (By similarity). Required for cellular differentiation in neuronal and myogenic lineages during embryonic development (PubMed:8878684).
(UniProt, P54361)
Summary (Interactive Fly)

modifies the activity of Ornithine decarboxylase - regulates the nuclear entry and overall levels of Sex-lethal in early germ cells.

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
3

Please see the GBrowse view of Dmel\Oda or the JBrowse view of Dmel\Oda for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.39

gene_with_transcript_with_translational_frameshift ; SO:0000712

Translational frameshifting postulated (FBrf0100578 and GB:AF038597): +1 frameshift reflected in aa sequence of predicted polypeptides.

Gene model reviewed during 5.56

+1 frameshift corresponds to genomic location 2R(r6):12,168,046.

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0088013
1736
254
FBtr0088015
1909
248
FBtr0088014
1471
270
Additional Transcript Data and Comments
Reported size (kB)

1.8 (northern blot)

2.1 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0087121
28.3
254
4.52
FBpp0087123
27.6
248
4.46
FBpp0087122
30.1
270
4.44
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

254 (aa); 28 (kD predicted)

186 (aa); 21 (kD predicted)

Comments
External Data
Subunit Structure (UniProtKB)

Interacts with ODC1 and thereby sterically blocks ODC homodimerization.

(UniProt, P54361)
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Oda using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: maternally deposited

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

guf transcripts are detected in embryonic RNA.

guf transcripts are detected throughout development on northern blots. Low levels of uniformly distributed guf RNA are detected in precellularized embryos by in situ hybridization. During gastrulation and germ band extension, transcripts are most prominent in the mesoderm and invaginating posterior midgut. In stage 11, expression is observed in fat body precursors, Malpighian tubule rudiments, and the foregut. At late stage 12, guf is expressed all over the embryo at low levels including the subectodermal layer that contains most of the PNS cells. Transcripts are first detected in developing body wall muscles in stage 14. Expression in the body wall muscles, fat body, and midgut is maintained throughout the rest of embryonic development.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from high throughput direct assay
inferred from high throughput direct assay
Expression Deduced from Reporters
Reporter: P{lacW}SmD3k09512
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lacW}SmD3k09540
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{lacW}SmD3k11803
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
Reporter: P{PZ}SmD302833
Stage
Tissue/Position (including subcellular localization)
Reference
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Oda in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 23 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Oda
Transgenic constructs containing regulatory region of Oda
Deletions and Duplications ( 1 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
No
Yes
7 of 15
No
Yes
1  
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
8 of 15
No
Yes
5 of 15
No
Yes
Rattus norvegicus (Norway rat) (3)
8 of 13
Yes
Yes
8 of 13
Yes
Yes
6 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
7 of 12
Yes
Yes
4 of 12
No
Yes
Danio rerio (Zebrafish) (4)
8 of 15
Yes
Yes
7 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
4 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
1 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190E23 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EEJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0HQT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Tribolium castaneum
Red flour beetle
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0HTE )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0SR0 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Subunit Structure (UniProtKB)
    Interacts with ODC1 and thereby sterically blocks ODC homodimerization.
    (UniProt, P54361 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map

    2-66

    Cytogenetic map
    Sequence location
    2R:12,166,295..12,174,264 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    48E4-48E4
    Limits computationally determined from genome sequence between P{lacW}jebk05644 and P{PZ}SmD302833
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    48E4-48E5
    (determined by in situ hybridisation)
    48E5-48E12
    (determined by in situ hybridisation)
    48E6-48E12
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (11)
    Genomic Clones (23)
    cDNA Clones (544)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    Other clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Additional comments

    The mutant phenotypes of the "l(2)k09540" and "l(2)k11803" lines were previously attributed to an effect on the Oda gene (FBrf0089808), however, evidence in FBrf0149027 shows that the mutant phenotype of these lines (and other P-element insertion lines located within an intron of Oda) is actually due to an effect on SmD3 (a gene nested in an intron of Oda) and not on Oda.

    Other Comments

    Oda encodes an antizyme that is apparently regulated by translational frameshifting.

    Two closely linked P-element transposon insertions cause the "guf" phenotype of embryonic lethality and aberrant neuronal and muscle cell differentiation. The insertions map to the 5' UTR of SmD3 and intron 1 of Oda. Defects in either SmD3 or Oda, or both, are likely causes of the phenotype.

    Origin and Etymology
    Discoverer
    Etymology

    The complementation group represented by "l(2)k11803", "l(2)k09540" and "l(2)k09512" is named "gutfeeling" because all the P{lacW} insertion mutants confer strong Ecol\lacZ expression in the gut and affect sensory organ development. FlyBase curator comment: the mutant phenotype of "l(2)k11803", "l(2)k09540" and "l(2)k09512" is attributed to an effect on the Oda gene in FBrf0089808 and the Oda gene is shown to be expressed in the gut in FBrf0089808. However, FBrf0149027 shows that the mutant phenotype of "l(2)k11803", "l(2)k09540" and "l(2)k09512" is actually due to an effect on SmD3 (a gene nested in an intron of Oda) and not on Oda.

    Identification
    External Crossreferences and Linkouts ( 53 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Synonyms and Secondary IDs (17)
    Reported As
    Name Synonyms
    Antizyme
    Ornithine Deacarboxylase Antizyme
    Ornithine decarboxylase antizyme
    ornithine decarboxylase antizyme
    orthinine decarboyxlase antizyme
    Secondary FlyBase IDs
    • FBgn0010521
    • FBgn0014270
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (81)