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Gene Dmel\Hel25E

General Information
Symbol	Dmel\Hel25E	Species	D. melanogaster
Name	Helicase at 25E	Annotation symbol	CG7269
Feature type	protein_coding_gene	FlyBase ID	FBgn0014189
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	UAP56, HEL, WM6, jf26
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	25E6-25E6	Sequence location	2L:5,539,326..5,542,310 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Helicase at 25E is referred to in FlyBase by the symbol Dmel\Hel25E (CG7269, FBgn0014189). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATP-dependent RNA helicase activity; RNA helicase activity. There is experimental evidence that it is involved in the biological process: mRNA export from nucleus; mitotic spindle organization; regulation of alternative nuclear mRNA splicing, via spliceosome. 12 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: DEAD-like helicase; DNA/RNA helicase, DEAD/DEAH box type, N-terminal; Helicase, C-terminal; RNA helicase, DEAD-box type, Q motif. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult testis, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1629620_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Hel25E. Gene sequence location is 2L:5539326..5542310.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features BKN29227 HFA03342
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 25E6-25E6   Limits computationally determined from genome sequence between P{lacW}vrik05901&P{PZ}cype03771 and P{lacW}Hel25Ek11511
Experimentally Determined Cytological Location
Cytogenetic map Notes References 25F-25F     (Warbrick, 1994.6.20) 25E-25E   (determined by in situ hybridisation)   (Lorenz, 1995.10.18) 25F-25F   (determined by in situ hybridisation)   (Warbrick and Glover, 1994)
Experimentally Determined Recombination Data
Location
Left of (cM)	mid (Kotarski et al., 1983)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Hel25E for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Hel25E-RA FBtr0079124  NM_164646   1592   424 Hel25E-RB FBtr0079125  NM_164647   1657   424 Hel25E-RC FBtr0079123  NM_164648   1756   424
Additional Transcript Data & Comments
Reported size (kB)	1.8, 1.6 (northern blot) (Eberl et al., 1997) 1.6 (longest cDNA) (Warbrick and Glover, 1994)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Hel25E-PA   FBpp0078755   48.7   424   5.37     NP_723089   AAF52261 Hel25E-PB   FBpp0078756   48.7   424   5.37     NP_723090   AAN10544 Hel25E-PC   FBpp0078754   48.7   424   5.37     NP_723091   AAN10545
Additional Polypeptide Data & Comments
Reported size (kDa)	424 (aa); 47 (kD observed) (Eberl et al., 1997) 424 (aa) (Warbrick and Glover, 1994)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Helicase, C-terminal (IPR001650) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) DEAD-like helicase (IPR014001) RNA helicase, DEAD-box type, Q motif (IPR014014)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202263   AA202332   AA202559   AA202671   AA263297   AA263351   AA264568   AA264885   AA264890   AA390545   AA391859   AA392492   AA439498   AA439686   AA439694   AA439698   AA439756   AA440092   AA441157   AA441303   AA441552   AA441666   AA441687   AA536409   AA539161   AA539751   AA539910   AA540136   AA540571   AA540655   AA540685   AA541167   AA567043   AA694925   AA695269   AA695728   AA801748   AA802778   AA803148   AA803252   AA816467   AA816477   AA817099   AA817182   AA817237   AA817378   AA820170   AA820545   AA940829   AA941634   AA942122   AA942130   AA942349   AA942351   AA948978   AA949967   AA950012   AA950031   AA951029   AA951340   AA951667   AA979319   AA979458   AE014134 AAF52261   AE014134 AAN10544   AE014134 AAN10545   AI257382   AI258049   AI295034   AI406123   AI455047   AI456192   AI456591   AI512696   AI513438   AI515551   AI515661   AI518035   AI518135   AI519902   AI520116   AI520248   AI533388   AI542280   AI543627   AI546453   AQ034028   AW942682   AY118921 AAM50781   AY231673   BF501418   BG638727   BI163470   BI164255   BI165411   BI165854   BI167798   BI168088   BI169478   BI170759   BI173718   BI215941   BI216216   BI216738   BI229774   BI230255   BI232946   BI235563   BI235873   BI238934   BI239697   BI241172   BI242542   BI351655   BI355441   BI355558   BI356069   BI356101   BI363176   BI363212   BI363647   BI364102   BI364282   BI365119   BI365665   BI365865   BI366581   BI366933   BI367024   BI367042   BI367717   BI369155   BI369744   BI369892   BI370026   BI370658   BI371294   BI372651   BI374385   BI374714   BI374750   BI375228   BI376029   BI376063   BI482825   BI482924   BI483071   BI485303   BI485605   BI485615   BI563482   BI565956   BI566144   BI568731   BI573660   BI573661   BI576579   BI579961   BI581566   BI583599   BI584926   BI585900   BI605104   BI615782   BI615910   BI620301   BI623463   BI628242   BI630160   BI632316   BI632575   BI634000   BI634011   BI634485   BI639532   BP555855   BP555856   BP559956   BP559957   BX005773   BX006184   BX007933   BX008715   BX009286   BX012341   BX015458   BX016175   BX016790   BX019862   BX021864   BX024138   BX026528   BX029113   BX038875   BX043738   BX051387   BX055209   BX055543   BX057437   BX058632   BX059268   BX061598   BX063591   BX071201   CA804231   CK131783   CK605384   CK605385   CK657580   CK659164   CO178967   CO179374   CO187397   CO187666   CO195926   CO267398   CO283686   CO292377   CO306408   CO308822   CO312586   CO315291   CO315507   CO317364   CO319122   CO341121   CZ468342   CZ480322   DQ440219   EC052299   EC054289   EC055192   EC065886   EC069092   EC076425   EC077805   EC078665   EC082613   EC086915   EC089273   EC195923   EC199147   EC199415   EC204138   EC207382   EC209131   EC212800   EC214857   EC215455   EC215894   EC217771   EC218667   EC224427   EC233437   EC235493   EC238093   EC238254   EC246377   EC250399   EC253444   EC264141   EV578286   EV580494   EV582046   EV584587   EV592873   GH942867   GH944487   GH972580   L06018 AAB65835   X79802 CAA56197
UniProtKB/Swiss-Prot	Q27268
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Eberl et al., 1997) gastrula stage   mitotic domain   (Eberl et al., 1997) larval stage   embryonic/larval salivary gland   (Eberl et al., 1997) adult stage & oogenesis   follicle cell   (Eberl et al., 1997) nurse cell   (Eberl et al., 1997)
Additional Descriptive Data
	In the larval salivary gland, Hel25E protein associates with the polytene chromosomes. In ovaries, staining is seen in nurse cell and follicle cell nuclei. Confocal microscopy of cycle 14 embyros double-labeled with antibodies against Hel25E and betaTub60D shows that Hel25E disappears from within mitotic domains, although it is present in the nuclei of the other cells at the surface of the embryo. (Eberl et al., 1997)
Marker for
Subcellular Localization
CV Term	nucleus polytene chromosome (Eberl et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0014189    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0014189 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, during early pupal stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0014189 expression level Developmental Stage   Expression Level embryo 00-02hr    29630 embryo 02-04hr    46158 embryo 04-06hr    58297 embryo 06-08hr    37290 embryo 08-10hr    30624 embryo 10-12hr    24456 embryo 12-14hr    15412 embryo 14-16hr    15562 embryo 16-18hr    11199 embryo 18-20hr    5827 embryo 20-22hr    13668 embryo 22-24hr    11088 larva L1    6665 larva L2    9850 larva L3 12hr old    6118 larva L3 puffstage 1-2    5081 larva L3 puffstage 3-6    6862 larva L3 puffstage 7-9    7733 white prepupae new    7730 white prepupae 12hr    8182 white prepupae 24hr    10150 pupae 2d postWPP    4550 pupae 3d postWPP    2308 pupae 4d postWPP    2456 adult male 01day    2057 adult male 05day    2419 adult male 30day    2602 adult female 01day    9421 adult female 05day    13868 adult female 30day    13826 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (29630) embryo 02-04hr  (46158) embryo 04-06hr  (58297) embryo 06-08hr  (37290) embryo 08-10hr  (30624) embryo 10-12hr  (24456) embryo 12-14hr  (15412) embryo 14-16hr  (15562) embryo 16-18hr  (11199) embryo 18-20hr  (5827) embryo 20-22hr  (13668) embryo 22-24hr  (11088) larva L1  (6665) larva L2  (9850) larva L3 12hr old  (6118) larva L3 puffstage 1-2  (5081) larva L3 puffstage 3-6  (6862) larva L3 puffstage 7-9  (7733) white prepupae new  (7730) white prepupae 12hr  (8182) white prepupae 24hr  (10150) pupae 2d postWPP  (4550) pupae 3d postWPP  (2308) pupae 4d postWPP  (2456) adult male 01day  (2057) adult male 05day  (2419) adult male 30day  (2602) adult female 01day  (9421) adult female 05day  (13868) adult female 30day  (13826) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (29630) embryo 02-04hr  (46158) embryo 04-06hr  (58297) embryo 06-08hr  (37290) embryo 08-10hr  (30624) embryo 10-12hr  (24456) embryo 12-14hr  (15412) embryo 14-16hr  (15562) embryo 16-18hr  (11199) embryo 18-20hr  (5827) embryo 20-22hr  (13668) embryo 22-24hr  (11088) larva L1  (6665) larva L2  (9850) larva L3 12hr old  (6118) larva L3 puffstage 1-2  (5081) larva L3 puffstage 3-6  (6862) larva L3 puffstage 7-9  (7733) white prepupae new  (7730) white prepupae 12hr  (8182) white prepupae 24hr  (10150) pupae 2d postWPP  (4550) pupae 3d postWPP  (2308) pupae 4d postWPP  (2456) adult male 01day  (2057) adult male 05day  (2419) adult male 30day  (2602) adult female 01day  (9421) adult female 05day  (13868) adult female 30day  (13826) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (29630) embryo 02-04hr  (46158) embryo 04-06hr  (58297) embryo 06-08hr  (37290) embryo 08-10hr  (30624) embryo 10-12hr  (24456) embryo 12-14hr  (15412) embryo 14-16hr  (15562) embryo 16-18hr  (11199) embryo 18-20hr    5827 embryo 20-22hr  (13668) embryo 22-24hr  (11088) larva L1    6665 larva L2    9850 larva L3 12hr old    6118 larva L3 puffstage 1-2    5081 larva L3 puffstage 3-6    6862 larva L3 puffstage 7-9    7733 white prepupae new    7730 white prepupae 12hr    8182 white prepupae 24hr  10150 pupae 2d postWPP    4550 pupae 3d postWPP    2308 pupae 4d postWPP    2456 adult male 01day    2057 adult male 05day    2419 adult male 30day    2602 adult female 01day    9421 adult female 05day  (13868) adult female 30day  (13826) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    29630 embryo 02-04hr    46158 embryo 04-06hr    58297 embryo 06-08hr    37290 embryo 08-10hr    30624 embryo 10-12hr    24456 embryo 12-14hr    15412 embryo 14-16hr    15562 embryo 16-18hr    11199 embryo 18-20hr    5827 embryo 20-22hr    13668 embryo 22-24hr    11088 larva L1    6665 larva L2    9850 larva L3 12hr old    6118 larva L3 puffstage 1-2    5081 larva L3 puffstage 3-6    6862 larva L3 puffstage 7-9    7733 white prepupae new    7730 white prepupae 12hr    8182 white prepupae 24hr    10150 pupae 2d postWPP    4550 pupae 3d postWPP    2308 pupae 4d postWPP    2456 adult male 01day    2057 adult male 05day    2419 adult male 30day    2602 adult female 01day    9421 adult female 05day    13868 adult female 30day    13826 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0014189 expression level Developmental Stage   Expression Level embryo 00-02hr    29630 embryo 02-04hr    46158 embryo 04-06hr    58297 embryo 06-08hr    37290 embryo 08-10hr    30624 embryo 10-12hr    24456 embryo 12-14hr    15412 embryo 14-16hr    15562 embryo 16-18hr    11199 embryo 18-20hr    5827 embryo 20-22hr    13668 embryo 22-24hr    11088 larva L1    6665 larva L2    9850 larva L3 12hr old    6118 larva L3 puffstage 1-2    5081 larva L3 puffstage 3-6    6862 larva L3 puffstage 7-9    7733 white prepupae new    7730 white prepupae 12hr    8182 white prepupae 24hr    10150 pupae 2d postWPP    4550 pupae 3d postWPP    2308 pupae 4d postWPP    2456 adult male 01day    2057 adult male 05day    2419 adult male 30day    2602 adult female 01day    9421 adult female 05day    13868 adult female 30day    13826 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (29630) embryo 02-04hr  (46158) embryo 04-06hr  (58297) embryo 06-08hr  (37290) embryo 08-10hr  (30624) embryo 10-12hr  (24456) embryo 12-14hr  (15412) embryo 14-16hr  (15562) embryo 16-18hr  (11199) embryo 18-20hr  (5827) embryo 20-22hr  (13668) embryo 22-24hr  (11088) larva L1  (6665) larva L2  (9850) larva L3 12hr old  (6118) larva L3 puffstage 1-2  (5081) larva L3 puffstage 3-6  (6862) larva L3 puffstage 7-9  (7733) white prepupae new  (7730) white prepupae 12hr  (8182) white prepupae 24hr  (10150) pupae 2d postWPP  (4550) pupae 3d postWPP  (2308) pupae 4d postWPP  (2456) adult male 01day  (2057) adult male 05day  (2419) adult male 30day  (2602) adult female 01day  (9421) adult female 05day  (13868) adult female 30day  (13826) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (29630) embryo 02-04hr  (46158) embryo 04-06hr  (58297) embryo 06-08hr  (37290) embryo 08-10hr  (30624) embryo 10-12hr  (24456) embryo 12-14hr  (15412) embryo 14-16hr  (15562) embryo 16-18hr  (11199) embryo 18-20hr  (5827) embryo 20-22hr  (13668) embryo 22-24hr  (11088) larva L1  (6665) larva L2  (9850) larva L3 12hr old  (6118) larva L3 puffstage 1-2  (5081) larva L3 puffstage 3-6  (6862) larva L3 puffstage 7-9  (7733) white prepupae new  (7730) white prepupae 12hr  (8182) white prepupae 24hr  (10150) pupae 2d postWPP  (4550) pupae 3d postWPP  2308 pupae 4d postWPP  2456 adult male 01day  2057 adult male 05day  2419 adult male 30day  (2602) adult female 01day  (9421) adult female 05day  (13868) adult female 30day  (13826) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (29630) embryo 02-04hr  (46158) embryo 04-06hr  (58297) embryo 06-08hr  (37290) embryo 08-10hr  (30624) embryo 10-12hr  (24456) embryo 12-14hr  15412 embryo 14-16hr  15562 embryo 16-18hr  11199 embryo 18-20hr    5827 embryo 20-22hr  13668 embryo 22-24hr  11088 larva L1    6665 larva L2    9850 larva L3 12hr old    6118 larva L3 puffstage 1-2    5081 larva L3 puffstage 3-6    6862 larva L3 puffstage 7-9    7733 white prepupae new    7730 white prepupae 12hr    8182 white prepupae 24hr  10150 pupae 2d postWPP    4550 pupae 3d postWPP    2308 pupae 4d postWPP    2456 adult male 01day    2057 adult male 05day    2419 adult male 30day    2602 adult female 01day    9421 adult female 05day  13868 adult female 30day  13826 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    29630 embryo 02-04hr    46158 embryo 04-06hr    58297 embryo 06-08hr    37290 embryo 08-10hr    30624 embryo 10-12hr    24456 embryo 12-14hr    15412 embryo 14-16hr    15562 embryo 16-18hr    11199 embryo 18-20hr    5827 embryo 20-22hr    13668 embryo 22-24hr    11088 larva L1    6665 larva L2    9850 larva L3 12hr old    6118 larva L3 puffstage 1-2    5081 larva L3 puffstage 3-6    6862 larva L3 puffstage 7-9    7733 white prepupae new    7730 white prepupae 12hr    8182 white prepupae 24hr    10150 pupae 2d postWPP    4550 pupae 3d postWPP    2308 pupae 4d postWPP    2456 adult male 01day    2057 adult male 05day    2419 adult male 30day    2602 adult female 01day    9421 adult female 05day    13868 adult female 30day    13826 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0014189    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0014189 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, larval hindgut, larval salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, adult testis, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0014189 expression level Tissue   Expression Level Larval Central Nervous System    1354.325 Larval Midgut    244.6 Larval Hindgut    514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland    1112.9 Larval Trachea    584.325 Larval Carcass    560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca    625 Adult InseminatedFemale Spermatheca    561.9 Adult Ovary    1592.2 Adult Testis    177.3 Adult Male Accessory Gland    658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1354.325) Larval Midgut    244.6 Larval Hindgut  514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland  (1112.9) Larval Trachea  (584.325) Larval Carcass  (560.925) Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca  (625) Adult InseminatedFemale Spermatheca  (561.9) Adult Ovary  (1592.2) Adult Testis    177.3 Adult Male Accessory Gland  (658) Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1354.325) Larval Midgut    244.6 Larval Hindgut    514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland  (1112.9) Larval Trachea    584.325 Larval Carcass    560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca    625 Adult InseminatedFemale Spermatheca    561.9 Adult Ovary  (1592.2) Adult Testis    177.3 Adult Male Accessory Gland    658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1354.325 Larval Midgut    244.6 Larval Hindgut    514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland    1112.9 Larval Trachea    584.325 Larval Carcass    560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca    625 Adult InseminatedFemale Spermatheca    561.9 Adult Ovary    1592.2 Adult Testis    177.3 Adult Male Accessory Gland    658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0014189 expression level Tissue   Expression Level Larval Central Nervous System    1354.325 Larval Midgut    244.6 Larval Hindgut    514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland    1112.9 Larval Trachea    584.325 Larval Carcass    560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca    625 Adult InseminatedFemale Spermatheca    561.9 Adult Ovary    1592.2 Adult Testis    177.3 Adult Male Accessory Gland    658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1354.325) Larval Midgut    244.6 Larval Hindgut  514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland  (1112.9) Larval Trachea  584.325 Larval Carcass  560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca  625 Adult InseminatedFemale Spermatheca  561.9 Adult Ovary  (1592.2) Adult Testis    177.3 Adult Male Accessory Gland  658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1354.325 Larval Midgut    244.6 Larval Hindgut    514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland  1112.9 Larval Trachea    584.325 Larval Carcass    560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca    625 Adult InseminatedFemale Spermatheca    561.9 Adult Ovary  1592.2 Adult Testis    177.3 Adult Male Accessory Gland    658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1354.325 Larval Midgut    244.6 Larval Hindgut    514.2 Larval Malpighian Tubules    391.8 Larval Fat Body    324.2 Larval Salivary Gland    1112.9 Larval Trachea    584.325 Larval Carcass    560.925 Adult Head    305.3 Adult Eye    269.025 Adult Brain    328.8 Adult Thoracic-Abdominal Ganglion    327 Adult Crop    377.4 Adult Midgut    266.1 Adult Hindgut    310.3 Adult Malpighian Tubules    358.4 Adult Fat Body    309.8 Adult Salivary Gland    195.1 Adult Heart    266.575 Adult VirginFemale Spermatheca    625 Adult InseminatedFemale Spermatheca    561.9 Adult Ovary    1592.2 Adult Testis    177.3 Adult Male Accessory Gland    658 Adult Carcass    242 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    305.3   NA  Eye    269.025   NA  Brain    328.8   1354.325  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    327   NA  Crop    377.4   244.6  Midgut    266.1   514.2  Hindgut    310.3   391.8  Malpighian Tubules    358.4   324.2  Fat Body    309.8   1112.9  Salivary Gland    195.1   NA  Heart    266.575   584.325  Trachea    NA   NA  VirginFemale Spermatheca    625   NA  InseminatedFemale Spermatheca    561.9   NA  Ovary    1592.2   NA  Testis    177.3   NA  Male Accessory Gland    658   560.925  Carcass    242 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014189 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7269-RC FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Hel25E Stages(s) 4-6 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 Hel25EGD12189 lethal | pupal stage, with Scer\GAL4pnr-MD237 Hel25EGD12189 lethal | recessive Hel25E1 Hel25E2 Hel25E3 Hel25Ek11511 viable Hel25Ee02545 Sterility Allele fertile Hel25Ee02545 Other Phenotypes Allele enhancer of variegation | dominant, with In(1)wm4 Hel25E3 suppressor of variegation, with In(1)wm4 Hel25E+t4.5 wild-type Hel25E+t4.5 Phenotype manifest in Allele adult abdomen | P-stage Hel25E3 adult head | P-stage Hel25E3 adult thorax | P-stage Hel25E3 dorsal appendage | germline clone Hel25E3 egg | germline clone Hel25E3 egg chamber | germline clone Hel25E3
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of Hel25E Class Mutagen Stocks Known lesion Hel25Ek11511 P-element activity 3 -- Hel25Ee02545 piggyBac transposase 2 -- Hel25E1 ethyl methanesulfonate 0 -- Hel25E2 ethyl methanesulfonate 0 -- Hel25E3 loss of function allele ethyl methanesulfonate 0 Yes Hel25Ec02934 piggyBac transposase 0 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Hel25E Class Mutagen Stocks Known lesion Hel25EGD12189 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Hel25EHMS00076 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hel25EKK109227 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Hel25E+t4.5 in vitro construct - genomic fragment 0 Yes Hel25EdsRNA.cHa in vitro construct - RNAi 0 Yes Hel25EdsRNA.N in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC169 (Christensen and Cook, 2006.7.11) Df(2L)BSC810 (Christensen et al., 2009.5.6) Df(2L)cl1 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)cl2 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)cl7 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)cl-h1 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Eberl et al., 1997) Df(2L)cl-h2 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Eberl et al., 1997) Df(2L)cl-h3 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)cl-h4 (Kotarski et al., 1983, Szidonya and Reuter, 1988, Szidonya and Reuter, 1988, Eberl et al., 1997) Df(2L)Exel6012 (Ryder, 2004.4.26) Df(2L)Exel7023 (Ryder, 2004.4.26) Df(2L)GpdhA (Kotarski et al., 1983) Df(2L)sc19-4 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-12 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Ts(YLt;2Lt)B137+Ts(YLt;2Rt)B236 (Kotarski et al., 1983)
Not disrupted in	Df(2L)2802 (Kotarski et al., 1983) Df(2L)Gpdh75 (Kotarski et al., 1983) Df(2L)Gpdh78 (Kotarski et al., 1983) Df(2L)sc19-1 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-5 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-6 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-7 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)sc19-8 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)tkv2 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) Df(2L)tkv3 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12189} NA P{KK109227} NA P{TRiP.HMS00076} NA characterization construct P{Hel25E+t4.5.w} NA P{Hel25E+t4.5.y} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Hel25Ek11511 No P{lacW}l(2)SH0642SH0642 No miscellaneous insertions PBac{PB}Hel25Ec02934 NA PBac{RB}Hel25Ee02545 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) mRNA export from nucleus inferred from mutant phenotype (Gatfield et al., 2001) regulation of alternative nuclear mRNA splicing, via spliceosome inferred from mutant phenotype (Park et al., 2004, Katzenberger et al., 2009)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Eberl et al., 1997)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) ATP-dependent RNA helicase activity inferred from sequence or structural similarity with EMBL:AF063447 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces SUB2 (Lasko, 2000) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) nucleic acid binding inferred from electronic annotation with InterPro:IPR001650, InterPro:IPR011545 (FlyBase Curators et al., 2004-) RNA helicase activity inferred from sequence or structural similarity with NCBI:NP_004631 (Mount and Salz, 2000)
Biological Process
CV term Evidence References
nuclear mRNA splicing, via spliceosome inferred from sequence or structural similarity with NCBI:NP_004631 AND inferred from sequence or structural similarity with Saccharomyces SUB2 (Mount and Salz, 2000)
Cellular Component
CV term Evidence References
nucleus inferred by curator from GO:0000381 (Park et al., 2004) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) spliceosomal complex inferred from sequence or structural similarity with Saccharomyces SUB2 (Mount and Salz, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Hel25E allele Gene References Hel25Ek11511 peb (Wilk et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59949 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0014189 DroID - A comprehensive database of gene and protein interactions. • FBgn0014189 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33781
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14747 Dere\GG24297 Dgri\GH11448 Dmoj\GI21901 Dper\GL25900 Dpse\GA20225 Dsec\GM18014 Dsim\GD11981 Dvir\GJ17817 Dwil\GK14902 Dyak\Hel25E
Curated drosophilid orthologs	Dpse\GA20225   Dyak\Hel25E
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL001216-PA Aedes aegypti (mosquito) AGAP009075-PA Anopheles gambiae (mosquito) XP_624894.1 Apis mellifera (honey bee) XP_001942765.1 Acyrthosiphon pisum (pea aphid) At5g11170.1 Arabidopsis thaliana (wall cress) At5g11200.1 Arabidopsis thaliana (wall cress) 15696 Batrachochytrium dendrobatidis (chytrid fungus) 118235 Branchiostoma floridae (lancelet) XP_001893112.1 Brugia malayi (roundworm nematode) BGIBMGA012772-PA Bombyx mori (silk worm) ENSBTAP00000029090 Bos taurus (bovine) CAL0000645 Candida albicans (a form of yeast) CN18039 Caenorhabditis brenneri (small nematode) CBP05782 Caenorhabditis briggsae (nematode) CE03025 Caenorhabditis elegans (worm) ENSCAFP00000000732 Canis familiaris (dog) CAGL0L06908g Candida glabrata (haploid yeast) Chro.80448 Cryptosporidium hominis (obligate parasite) ENSCINP00000010722 Ciona intestinalis (sea squirt) JA09467 Caenorhabditis japonica (nematode) CME073C Cyanidioschyzon merolae (red alga) cn01710 Cryptococcus neoformans (yeast-like fungus) cgd8_3900 Cryptosporidium parvum (protozoan parasite) CPIJ014241 Culex pipens (mosquito) ENSCPOP00000002121 Cavia porcellus (guinea pig) ENSCPOP00000007741 Cavia porcellus (guinea pig) 83282 Chlamidomonas reinhardtii (green alga) RP31278 Caenorhabditis remanei (nematode) ENSCSAVP00000003651 Ciona savignyi (sedentary tunicate) 165082 Capitella spl (polychaete worm) DDB0233387 Dictyostelium discoideum (amoeba) DEHA2F06138g Debaryomyces Hansenii (yeast) 219465 Daphnia pulex (daphnia) ENSDARP00000010925 Danio rerio (zebra fish) ENSECAP00000006173 Equus caballus (horse) XP_655794 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000025568 Gymnopilus aculeatus (mushroom) GL50803_16376 Giardia lamblia (flagellated protozoan) 112849 Hypsipyla robusta (cedar shoot borer) ENSP00000373000 Homo sapiens (human) ENSP00000379475 Homo sapiens (human) ENSP00000383117 Homo sapiens (human) KLLA0E21935g Kluyveromyces lactis (yeast) 225638 Lottia gigantea (owl limpet) LmjF21.1552 Leishmania major (virus) 36918 Monocosta brevicollis (choanoflagellate) ENSMODP00000002973 Monodelphis domestica (gray opossum) ENSMMUP00000034109 Macaca mulatta (rhesus monkey) ENSMUSP00000070682 Mus musculus (house mouse) 236766 Nematostella vectensis (starlet anemone) XP_001606535.1 Nasonia vitripennis (wasp) ENSOANP00000008158 Ornithorynchus anatinus (platypus) ENSORLP00000013245 Oryzias latipes (japanese killifish) LOC_Os01g36890.1 Oryza sativa (asian rice) LOC_Os01g36920.1 Oryza sativa (asian rice) 21299 Ostreococcus tauri (green alga) PFB0445c Plasmodium falciparum (protozoal parasite) PHUM001432-PA Pediculus humanus (human body louse) PP20899 Pristionchus pacificus (nematode) 188774 Physcomitrella patens (moss) ENSPPYP00000018374 Pongo pygmaeus (orangutan) 71909 Phytophthora ramorum (sudden oak death pathogen) 108807 Phytophthora sojae (root rot pathogen) 578196 Populus trichocarpa (black cottonwood) 819597 Populus trichocarpa (black cottonwood) ENSPTRP00000054521 Pan troglodytes (chimpanzee) PVX_003945 Plasmodium vivax (protozoal parasite) ENSRNOP00000001115 Rattus norvegicus (norway rat) 4769245 Sorghum bicolor (sorghum) YDL084W Saccharomyces cerevisiae (budding yeast) Smp_003660 Schistosoma mansoni (trematode parasite) SPAC17G6.14c Schizosaccharomyces pombe (fission yeast) XP_001177126.1 Strongylocentrotus purpuratus (purple sea urchin) 63649 Trichoplax adhaerens (metazoa) TA04190 Theileria annulata (apicomplexan parasite) XP_974045.1 Tribolium castaneum (red flour beetle) Tc00.1047053508319.40 Trypanosoma cruzi (parasitic trypanosome) TP03_0373 Theileria parva (parasitic protozoan) 269556 Thalassiosira pseudonana (marine diatom) ENSTRUP00000034304 Takifugu rubripes (pufferfish Takifugu) 3710.m00143 Tetrahymena thermophila (ciliate protozoa) 86393.m00077 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000044525 Xenopus tropicalis (frog) YALI0A11157g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11157 PB11154 PB10544 NV50364 NV14889 NV14887 NV12200 LH23726 ISCW010314 FBgn0068148 FBgn0216907 FBgn0204985 FBgn0183720 FBgn0172921 JGI_V11_309072 JGI_V11_219465 FBgn0080220 FBgn0163484 FBgn0144630 FBgn0118928 FBgn0116429 FBgn0091774 CPIJ014241 BGIBMGA012772 ACYPI003124 GB13214 AGAP009075 AAEL001216 PHUM136930 GLEAN_14413
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	18062 w1118; PBac{RB}Hel25Ee02545 33666 y1 sc* v1; P{TRiP.HMS00076}attP2 11043 y1 w67c23; P{lacW}Hel25Ek11511/CyO
Harvard	- PBac{RB}Hel25Ee02545
Kyoto	108861 y1 w67c23; P{lacW}Hel25Ek11511/CyO 111310 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Hel25Ek11511 P{neoFRT}40A/CyO y+
VDRC	v104481 P{KK109227}VIE-260B v22557 w1118; P{GD12189}v22557 v22556 w1118; P{GD12189}v22556/TM3
Genomic Clones ( 2 )
	BACR35L07 BACR44J04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 201 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD23644
Other clones	DPiM_LD23644 yak-em_Hel25E
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT16734 GM01003 GM02511 GM03443 GM04380 GM06295 GM10311 GM10461 GM15539 GM30789 GM31377 LD01896 LD02384 LD02472 LD02742 LD02920 LD06391 LD06408 LD07062 LD07680 LD09403 LD09512 LD09591 LD10756 LD11368 LD14082 LD14319 LD14329 LD14333 LD14404 LD15173 LD16054 LD16188 LD16694 LD17010 LD17258 LD18072 LD19209 LD19520 LD20295 LD20418 LD21001 LD22187 LD22354 LD22431 LD22644 LD23082 LD24333 LD25876 LD26169 LD26504 LD26506 LD27695 LD28550 LD29949 LD29977 LD31288 LD31754 LD32318 LD33814 LD36060 LD36658 LD37438 LD37560 LD39739 LD40093 LD40344 LD44648 LD45710 LD47565 LP05728 LP08546 LP12786 LP16728 LP23990 RE02871 RE05304 RE08631 RE27627 RE29203 RE36266 RE38189 RE47928 RE47971 RE48553 RE49357 RE51042 RE51257 RE52154 RE62979 RE65590 RE65733 RE65911 RE68609 RE68969 RE68980 RE74071 RH22252 RH25595 RH33168 RH35002 RH38650 RH40416 RH45539 RH51389 RH55726 RH56592 RH59144 RH70631 SD05178 SD08525 SD10472 SD22132 SD26065 SD26389 SD28228 SD28239
Other clones	Dmel_dig_003093_0707_0498 Dmel_dig_010314_0660_1726 Dmel_dig_011297_0597_1571 Dmel_dig_019429_0681_0883 Dmel_neb_014905_0677_0892 EC43555 EC052299 EC054289 EC055192 EK028947 EK031205 EK058057 EK059237 EK150029 EK164101 EK200026 EK229346 EK250961 EK259443 EK260961 EK267819 EK276327 EP11913 ESG0112c.B21_G03 FGM237E09 FGM323H02 GH26327 GM01792 LD01831 LD16529 LD16670 LD20456 LD26179 LD34040 LD47430 LD48031 LP01135 RE03902 RE05836 RE08267 RE08930 RE10664 RE12332 RE16807 RE22719 RE23162 RE23975 RE31423 RE31823 RE35307 RE39913 RE42107 RE42148 RE49147 RE50349 RE52574 RE52691 RE52714 RE53526 RE55257 RE56029 RE56229 RE56389 RE57370 RE58158 RE59665 RE61961 RE62379 RE62423 RE63949 RE63988 RH09218 RH19079 RH24260 RH34124 RH35293 RH45808 RP004240858 RP004257197 RP004395250 UT01890
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0014189 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33781
Antibody Information
	polyclonal (Eberl et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Hel25E CG7269 (Skupski, 1999.11)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells. (Nybakken et al., 2005) Hel25E encodes a putative RNA helicase that contains an unusual variant (DECD) of the DEAD motif common to these proteins. (Eberl et al., 1997) Deletion or mutation of the Hel25E locus enhances position effect variegation of w. (Eberl et al., 1997) Hel25E gene can influence position effect variegation (PEV). Loss of one or two copies enhances PEV of a variegating allele of the w gene, complete removal of Hel25E is lethal. (Lorenz et al., 1995) S.pombe Spom\wee1 Spom\mik1 double mutants are functionally complemented by Hel25E. (Warbrick and Glover, 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_164646 NM_164647 NM_164648 RefSeq (Proteins) • NP_723089 NP_723090 NP_723091 DDBJ / EMBL / GenBank • AA820170 AAB65835 AAF52261 AAM50781 AAN10544 AAN10545 AQ034028 AW942682 AY118921 CAA56197 CZ468342 CZ480322 L06018 X79802 Entrez Gene - A searchable database of RefSeq genes. • 33781 UniProtKB/Swiss-Prot • Q27268
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Helicase, C-terminal (IPR001650) DNA/RNA helicase, DEAD/DEAH box type, N-terminal (IPR011545) DEAD-like helicase (IPR014001) RNA helicase, DEAD-box type, Q motif (IPR014014)
Linkouts
	BioGRID - A database of protein and genetic interactions • 59949 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0014189 DroID - A comprehensive database of gene and protein interactions. • FBgn0014189 DRSC - Results from RNAi screens. • FBgn0014189 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014189 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7269-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0014189 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33781 InParanoid A subset of ortholog calls from InParanoid. • AAEL001216-PA Aedes aegypti (mosquito) AGAP009075-PA Anopheles gambiae (mosquito) XP_624894.1 Apis mellifera (honey bee) XP_001942765.1 Acyrthosiphon pisum (pea aphid) At5g11170.1 Arabidopsis thaliana (wall cress) At5g11200.1 Arabidopsis thaliana (wall cress) 15696 Batrachochytrium dendrobatidis (chytrid fungus) 118235 Branchiostoma floridae (lancelet) XP_001893112.1 Brugia malayi (roundworm nematode) BGIBMGA012772-PA Bombyx mori (silk worm) ENSBTAP00000029090 Bos taurus (bovine) CAL0000645 Candida albicans (a form of yeast) CN18039 Caenorhabditis brenneri (small nematode) CBP05782 Caenorhabditis briggsae (nematode) CE03025 Caenorhabditis elegans (worm) ENSCAFP00000000732 Canis familiaris (dog) CAGL0L06908g Candida glabrata (haploid yeast) Chro.80448 Cryptosporidium hominis (obligate parasite) ENSCINP00000010722 Ciona intestinalis (sea squirt) JA09467 Caenorhabditis japonica (nematode) CME073C Cyanidioschyzon merolae (red alga) cn01710 Cryptococcus neoformans (yeast-like fungus) cgd8_3900 Cryptosporidium parvum (protozoan parasite) CPIJ014241 Culex pipens (mosquito) ENSCPOP00000002121 Cavia porcellus (guinea pig) ENSCPOP00000007741 Cavia porcellus (guinea pig) 83282 Chlamidomonas reinhardtii (green alga) RP31278 Caenorhabditis remanei (nematode) ENSCSAVP00000003651 Ciona savignyi (sedentary tunicate) 165082 Capitella spl (polychaete worm) DDB0233387 Dictyostelium discoideum (amoeba) DEHA2F06138g Debaryomyces Hansenii (yeast) 219465 Daphnia pulex (daphnia) ENSDARP00000010925 Danio rerio (zebra fish) ENSECAP00000006173 Equus caballus (horse) XP_655794 Entamoeba histolytica (anaerobic protozoan) ENSGACP00000025568 Gymnopilus aculeatus (mushroom) GL50803_16376 Giardia lamblia (flagellated protozoan) 112849 Hypsipyla robusta (cedar shoot borer) ENSP00000373000 Homo sapiens (human) ENSP00000379475 Homo sapiens (human) ENSP00000383117 Homo sapiens (human) KLLA0E21935g Kluyveromyces lactis (yeast) 225638 Lottia gigantea (owl limpet) LmjF21.1552 Leishmania major (virus) 36918 Monocosta brevicollis (choanoflagellate) ENSMODP00000002973 Monodelphis domestica (gray opossum) ENSMMUP00000034109 Macaca mulatta (rhesus monkey) ENSMUSP00000070682 Mus musculus (house mouse) 236766 Nematostella vectensis (starlet anemone) XP_001606535.1 Nasonia vitripennis (wasp) ENSOANP00000008158 Ornithorynchus anatinus (platypus) ENSORLP00000013245 Oryzias latipes (japanese killifish) LOC_Os01g36890.1 Oryza sativa (asian rice) LOC_Os01g36920.1 Oryza sativa (asian rice) 21299 Ostreococcus tauri (green alga) PFB0445c Plasmodium falciparum (protozoal parasite) PHUM001432-PA Pediculus humanus (human body louse) PP20899 Pristionchus pacificus (nematode) 188774 Physcomitrella patens (moss) ENSPPYP00000018374 Pongo pygmaeus (orangutan) 71909 Phytophthora ramorum (sudden oak death pathogen) 108807 Phytophthora sojae (root rot pathogen) 578196 Populus trichocarpa (black cottonwood) 819597 Populus trichocarpa (black cottonwood) ENSPTRP00000054521 Pan troglodytes (chimpanzee) PVX_003945 Plasmodium vivax (protozoal parasite) ENSRNOP00000001115 Rattus norvegicus (norway rat) 4769245 Sorghum bicolor (sorghum) YDL084W Saccharomyces cerevisiae (budding yeast) Smp_003660 Schistosoma mansoni (trematode parasite) SPAC17G6.14c Schizosaccharomyces pombe (fission yeast) XP_001177126.1 Strongylocentrotus purpuratus (purple sea urchin) 63649 Trichoplax adhaerens (metazoa) TA04190 Theileria annulata (apicomplexan parasite) XP_974045.1 Tribolium castaneum (red flour beetle) Tc00.1047053508319.40 Trypanosoma cruzi (parasitic trypanosome) TP03_0373 Theileria parva (parasitic protozoan) 269556 Thalassiosira pseudonana (marine diatom) ENSTRUP00000034304 Takifugu rubripes (pufferfish Takifugu) 3710.m00143 Tetrahymena thermophila (ciliate protozoa) 86393.m00077 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000044525 Xenopus tropicalis (frog) YALI0A11157g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/uap56_helicase25e.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33781 modMine - Data generated by the modENCODE project. • FBgn0014189 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB11157 PB11154 PB10544 NV50364 NV14889 NV14887 NV12200 LH23726 ISCW010314 FBgn0068148 FBgn0216907 FBgn0204985 FBgn0183720 FBgn0172921 JGI_V11_309072 JGI_V11_219465 FBgn0080220 FBgn0163484 FBgn0144630 FBgn0118928 FBgn0116429 FBgn0091774 CPIJ014241 BGIBMGA012772 ACYPI003124 GB13214 AGAP009075 AAEL001216 PHUM136930 GLEAN_14413
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	CG7269 (Fulton et al., 2001, Mount and Salz, 2000, Nybakken et al., 2005, Goshima et al., 2007, Katzenberger et al., 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Ni et al., 2010.12.1) Dbp25F   DmRH4 (Boudet et al., 2001) HEL (Gatfield et al., 2001) hel (Wallrath, 1998, Eberl et al., 1997, Lorenz et al., 1995) Hel   Hel25E (Christensen and Cook, 2006.7.11, Nybakken et al., 2005, Goshima et al., 2007, Meignin and Davis, 2008, Katzenberger et al., 2009, Christensen et al., 2009.5.6, Dworkin et al., 2009, Nie et al., 2009, Gesellchen et al., 2005, Zimek and Weber, 2011) jf26 (Szidonya and Reuter, 1988, Szidonya and Reuter, 1988) l(2)25Eb   l(2)gdh-12 (Kotarski et al., 1983, ) l(2)gdh12   l(2)jf26   l(2)k11511 (Meignin and Davis, 2008) sz (Meignin and Davis, 2008) UAP56 (Hieronymus and Silver, 2004, Herold et al., 2003, Gatfield and Izaurralde, 2002, Meignin and Davis, 2008, Farny et al., 2008, Katzenberger et al., 2009) uap56 (Meignin and Davis, 2005, Meignin and Davis, 2008) WM6 (Warbrick, 1994.6.20, Eisen et al., 1998, Warbrick and Glover, 1994) Wm6
Name Synonym	helicase (Lorenz et al., 1995) Helicase at 25E   lethal(2)25Eb   RNA helicase (Melnick, 1992.11.9)
Secondary FlyBase IDs
	FBgn0001929 FBgn0008648 FBgn0021910
References ( 64 )
Generate a list of	All references relating to this gene ( 64 )
List References by type	Research paper  ( 30 ) Supplementary material  ( 5 ) Review  ( 2 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 2 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Book  ( 1 )
Recent research papers ( 2 )
	Zimek and Weber, 2011, Europ. J. Cell Biol. 90(4): 356--364 Flanking genes of an essential gene give information about the evolution of metazoa. [FBrf0213069] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...