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Gene Dmel\mia

General Information
Symbol	Dmel\mia	Species	D. melanogaster
Name	meiosis I arrest	Annotation symbol	CG10390
Feature type	protein_coding_gene	FlyBase ID	FBgn0014342
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	TFIID, Taf6L, Taf60-2
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	83B1-83B1	Sequence location	3R:1,374,048..1,376,889 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene meiosis I arrest is referred to in FlyBase by the symbol Dmel\mia (CG10390, FBgn0014342). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: male meiosis I; spermatid differentiation; regulation of gene expression; spermatogenesis. 8 alleles are reported. The phenotypes of these alleles are annotated with: testis; spermatocyte. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Domain of unknown function DUF1546. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut. Comments on Affy2 ProbeSet: ProbeSet 1635813_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of mia. Gene sequence location is 3R:1374048..1376889.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features HFA12137 BKN27112 Controlled Vocabulary Terms spermatid differentiation regulation of gene expression male germ cell nucleus
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 83B1-83B1   Limits computationally determined from genome sequence between P{PZ}Snr101319&P{PZ}Itp-r83A05616 and P{lacW}Atus1938
Experimentally Determined Cytological Location
Cytogenetic map Notes References   Placed in 78C9--81F or 83A--C2 based on complementation by Df(3L)ri-79c, Df(3L)Pc-MK, Df(3R)2-2, and Df(3R)Tpl10.   (Lin, 1995.4.19)
Experimentally Determined Recombination Data
Location	3-47.0 +/- 0.2 (Lin, 1995.4.19, Lin et al., 1996)
Left of (cM)	Ki (Lin, 1995.4.19, Lin et al., 1996)
Right of (cM)	kni (Lin, 1995.4.19, Lin et al., 1996)
Notes	Mapping based on 14 recombinants between kni and Ki. (Lin et al., 1996)
Gene Model & Products
Please see the GBrowse view of Dmel\mia for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 DGC clone LD25013 appears problematic: unspliced introns (genomic contamination?)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mia-RA FBtr0089313  NM_141290   2378   589 mia-RB FBtr0300009  NM_001144538   2347   589
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mia-PA   FBpp0088355   66.0   589   9.72     NP_649547   AAF52013 mia-PB   FBpp0289286   66.0   589   9.72     NP_001138010   ACL83469
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF1546 (IPR011442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA941062   AE014297 AAF52013   AE014297 ACL83469   AW942715   AY060376 AAL25415   AY752878 AAV33343   BF487775   BI142021   BI635783   BT001890 AAN71679   BT029284   CL528068   EC087874   GH942646   GH947217   GH947454   GH949219   GH951439   GH963041   GH964116   GH965245   GH965456   GH968258   GH973426   GH981856   GH981888
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q5UEC4 Q8IG94 Q95T18 Q9VND0
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0014342    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0014342 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0014342 expression level Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr  (246) embryo 04-06hr  (358) embryo 06-08hr  (501) embryo 08-10hr  (338) embryo 10-12hr  (142) embryo 12-14hr  107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1  (135) larva L2  (174) larva L3 12hr old  105 larva L3 puffstage 1-2  (119) larva L3 puffstage 3-6  (194) larva L3 puffstage 7-9  (210) white prepupae new  (176) white prepupae 12hr  (385) white prepupae 24hr  (334) pupae 2d postWPP  (219) pupae 3d postWPP  (181) pupae 4d postWPP  (202) adult male 01day  (247) adult male 05day  (361) adult male 30day  (330) adult female 01day  (125) adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0014342 expression level Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr  (246) embryo 04-06hr  (358) embryo 06-08hr  (501) embryo 08-10hr  (338) embryo 10-12hr  142 embryo 12-14hr  107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1  135 larva L2  (174) larva L3 12hr old  105 larva L3 puffstage 1-2  119 larva L3 puffstage 3-6  (194) larva L3 puffstage 7-9  (210) white prepupae new  (176) white prepupae 12hr  (385) white prepupae 24hr  (334) pupae 2d postWPP  (219) pupae 3d postWPP  (181) pupae 4d postWPP  (202) adult male 01day  (247) adult male 05day  (361) adult male 30day  (330) adult female 01day  125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    52 embryo 02-04hr    246 embryo 04-06hr    358 embryo 06-08hr    501 embryo 08-10hr    338 embryo 10-12hr    142 embryo 12-14hr    107 embryo 14-16hr    61 embryo 16-18hr    73 embryo 18-20hr    66 embryo 20-22hr    72 embryo 22-24hr    92 larva L1    135 larva L2    174 larva L3 12hr old    105 larva L3 puffstage 1-2    119 larva L3 puffstage 3-6    194 larva L3 puffstage 7-9    210 white prepupae new    176 white prepupae 12hr    385 white prepupae 24hr    334 pupae 2d postWPP    219 pupae 3d postWPP    181 pupae 4d postWPP    202 adult male 01day    247 adult male 05day    361 adult male 30day    330 adult female 01day    125 adult female 05day    97 adult female 30day    70 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0014342    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0014342 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0014342 expression level Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0014342 expression level Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules no informative data Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    20.2   NA  Eye    23.375   NA  Brain    16.3   30.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    29.5   NA  Crop    20   78.3  Midgut    161.6   38.1  Hindgut    18.1   22.3  Malpighian Tubules    no informative data   37.4  Fat Body    16.7   24.1  Salivary Gland    39   NA  Heart    12.05   37.65  Trachea    NA   NA  VirginFemale Spermatheca    16   NA  InseminatedFemale Spermatheca    22.4   NA  Ovary    19.3   NA  Testis    73.8   NA  Male Accessory Gland    29.5   19.3  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_21
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014342 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10390-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable miaEY07883 viable, with Scer\GAL4pnr-MD237 miaGD4141 viable (with mia1) miaz-3348 viable (with miaz-3348) mia1 Sterility Allele fertile miaEY07883 fertile (with mia1) miaz-3348 fertile (with miaz-3348) mia1 male sterile | recessive mia1 Phenotype manifest in Allele spermatocyte mia1 testis mia1
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of mia Class Mutagen Stocks Known lesion miaB560 1 -- miaEY07883 P-element activity 1 -- miaLT P-element activity 1 Yes mia1 ethyl methanesulfonate 0 Yes miaunspecified 0 -- miaz-3348 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 2 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mia Class Mutagen Stocks Known lesion miaGD4141 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes miaKK107112 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3R)LT (O'Connor and Tanini, 2009.6.24)
Disrupted in	Df(3R)Exel6144 (Ryder, 2004.4.26)
Not disrupted in	Df(3L)Pc-MK (Lin, 1995.4.19, Lin et al., 1996) Df(3L)ri-79c (Lin, 1995.4.19, Lin et al., 1996) Df(3R)2-2 (Lin, 1995.4.19, Lin et al., 1996) Df(3R)Tpl10 (Lin, 1995.4.19, Lin et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4141} NA P{KK107112} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}miaEY07883 NA miscellaneous insertions P{3'EPgy2}LT NA PBac{5HPw+}miaB560 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
male meiosis I inferred from mutant phenotype (Lin et al., 1996) regulation of gene expression inferred from mutant phenotype (White-Cooper et al., 1998) spermatid differentiation inferred from mutant phenotype (White-Cooper et al., 1998) spermatogenesis inferred from mutant phenotype (Lin et al., 1996)
Cellular Component
CV term Evidence References
male germ cell nucleus inferred from direct assay (Chen et al., 2005) nucleolus inferred from direct assay (Chen et al., 2005)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
meiosis traceable author statement (Fuller, 1998) regulation of sequence-specific DNA binding transcription factor activity inferred from electronic annotation with InterPro:IPR011442 (FlyBase Curators et al., 2004-) spermatid development traceable author statement (Fuller, 1998) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human TAF6L AND inferred from sequence or structural similarity with Saccharomyces TAF60 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
transcription factor TFIID complex inferred from sequence or structural similarity with human TAF6L AND inferred from sequence or structural similarity with Saccharomyces TAF60 (Aoyagi and Wassarman, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mia allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0014342 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40667
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16042 Dere\GG10968 Dgri\GH17238 Dmoj\GI24488 Dsec\GM10624 Dsim\GD19609 Dvir\GJ24553 Dwil\GK13782 Dyak\GE24166
Curated drosophilid orthologs
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • JA18671 Caenorhabditis japonica (nematode) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0241298 FBgn0215790 FBgn0211633 FBgn0191101 FBgn0165570 FBgn0147210 FBgn0103271 FBgn0093065
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	16865 y1 w67c23; P{EPgy2}miaEY07883 27595 y1 w*; Df(3R)LT, miaLT PcmtLT 16244 y1 w1118; PBac{5HPw+}miaB560/TM3, Sb1 Ser1
VDRC	v100313 P{KK107112}VIE-260B v37563 w1118; P{GD4141}v37563/CyO
Genomic Clones ( 1 )
	BACR24O24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 19 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD25013 SD16013
Other clones	RT01020
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT22828 SD17264
Other clones	104186 RP004161087 RP004187319 RP004209461 RP004211974 RP004226088 RP004227295 RP004265187 RP004381877 RP004457210 RP004491806 RP004495514 RP004508084 RP004518985
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0014342 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40667
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Taf60-2 CG10390 (Aoyagi and Wassarman, 2000)
Source for database merge of	Source for merge of: mia Taf6L (Hiller et al., 2004)
Additional comments
Other Comments
	can, mia and sa are required for accumulation of tweT:Ecol\lacZ transgene in late primary spermatocytes. (White-Cooper et al., 1998) Wild type function of aly, can, mia and sa is required for cell-cycle progression through the G2/M transition of meiosis I in males and for onset of spermatid differentiation. The phenotype of these meiotic arrest mutants is strikingly similar to the histopathological features of meiosis I maturation arrest infertility in human males, suggesting that the control point may be conserved from flies to man. (Lin et al., 1996) mia is required for both the progression of the male meiotic cell cycle and the onset of postmeiotic differentiation. (Lin et al., 1995) Mutations in mia cause a complete arrest of spermatogenesis at the G2/M transition to meiosis I, similar to aly, can, and sa. (Lin, 1995.4.19)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141290 NM_001144538 RefSeq (Proteins) • NP_649547 NP_001138010 DDBJ / EMBL / GenBank • AA941062 AAF52013 AAL25415 AAN71679 AAV33343 ACL83469 AW942715 AY060376 AY752878 BI142021 BT001890 CL528068 Entrez Gene - A searchable database of RefSeq genes. • 40667 UniProtKB/TrEMBL • Q5UEC4 Q8IG94 Q9VND0 Q95T18
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF1546 (IPR011442)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0014342 DRSC - Results from RNAi screens. • FBgn0014342 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014342 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10390-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0014342 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40667 InParanoid A subset of ortholog calls from InParanoid. • JA18671 Caenorhabditis japonica (nematode) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40667 modMine - Data generated by the modENCODE project. • FBgn0014342 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0241298 FBgn0215790 FBgn0211633 FBgn0191101 FBgn0165570 FBgn0147210 FBgn0103271 FBgn0093065
Synonyms & Secondary IDs ( 20 )
Reported As
Symbol Synonym	CG10390 (Labourier et al., 2002, Keleman et al., 2009.8.5, Aoyagi and Wassarman, 2001) dmTAF6b (Hochheimer and Tjian, 2003) dTAF6L (Hiller et al., 2004) dTAFII60 (Hochheimer and Tjian, 2003) dTAFII60-2 (Veenstra and Wolffe, 2001) mia (Ceulemans, 2006, Mikhaylova et al., 2006, Arya et al., 2006, Jiang et al., 2007, Chen et al., 2005, Chen et al., 2005, Metcalf and Wassarman, 2007, Yakoby et al., 2008, Moon et al., 2011, O'Connor and Tanini, 2009.6.24, Weake et al., 2009, Di Cara et al., 2010, Janic et al., 2010) ms(3)570   TAF (Mueller and Tora, 2004) Taf6L (Bloomington Drosophila Stock Center, 2004.11.7, ) TAF6L (Tora, 2002, Weake et al., 2009) Taf60-2 (Orian et al., 2003, ) TAF60-2 (Aoyagi and Wassarman, 2000) TAFII (Arnosti, 2002) TAFII60-2 (Aoyagi and Wassarman, 2001) TFIID (Butler and Kadonaga, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001)
Name Synonym	meiosis I arrest (Moon et al., 2011, Weake et al., 2009) Meiosis I arrest (Metcalf and Wassarman, 2007) TAF6-like (Hiller et al., 2004.9.14) TBP-associated factor 6-like   TBP-associated factor 60kD-2
Secondary FlyBase IDs
	FBgn0037337
References ( 62 )
Generate a list of	All references relating to this gene ( 62 )
List References by type	Research paper  ( 25 ) Supplementary material  ( 3 ) Review  ( 9 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 10 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 2 ) Curated genome annotation  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 3 )
	Moon et al., 2011, Development 138(17): 3835--3845 The THO complex is required for nucleolar integrity in Drosophila spermatocytes. [FBrf0214600] Di Cara et al., 2010, Insect Mol. Biol. 19(6): 807--813 Expressional and functional analysis of the male-specific cluster mst36F during Drosophila spermatogenesis. [FBrf0212329] Janic et al., 2010, Science 330(6012): 1824--1827 Ectopic expression of germline genes drives malignant brain tumor growth in Drosophila. [FBrf0212713] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...