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Gene Dmel\mia

General Information
Symbol	Dmel\mia	Species	D. melanogaster
Name	meiosis I arrest	Annotation symbol	CG10390
Feature type	protein_coding_gene	FlyBase ID	FBgn0014342
Gene Model Status	Current	Stock availability	5 publicly available
Also Known As	TFIID, Taf6L, Taf60-2
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	83B1-83B1	Sequence location	3R:1,373,837..1,376,354 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene meiosis I arrest is referred to in FlyBase by the symbol Dmel\mia (CG10390, FBgn0014342). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: male meiosis I; spermatid differentiation; regulation of gene expression; spermatogenesis. 9 alleles are reported. The phenotypes of these alleles are annotated with: testis; spermatocyte. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Armadillo-like helical; Domain of unknown function DUF1546. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut. Comments on Affy2 ProbeSet: ProbeSet 1635813_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of mia. Gene sequence location is 3R:1373837..1376354.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 83B1-83B1   Limits computationally determined from genome sequence between P{PZ}Snr101319&P{PZ}Itp-r83A05616 and P{lacW}Atus1938
Experimentally Determined Cytological Location
Cytogenetic map Notes References   Placed in 78C9--81F or 83A--C2 based on complementation by Df(3L)ri-79c, Df(3L)Pc-MK, Df(3R)2-2, and Df(3R)Tpl10.   (Lin, 1995.4.19)
Experimentally Determined Recombination Data
Location	3-47.0 +/- 0.2 (Lin, 1995.4.19, Lin et al., 1996)
Left of (cM)	Ki (Lin, 1995.4.19, Lin et al., 1996)
Right of (cM)	kni (Lin, 1995.4.19, Lin et al., 1996)
Notes	Mapping based on 14 recombinants between kni and Ki. (Lin et al., 1996)
Gene Model & Products
Please see the GBrowse view of Dmel\mia for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.48 DGC clone LD25013 appears problematic: unspliced introns (genomic contamination?)
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mia-RA FBtr0089313  NM_141290   2054   589 mia-RB FBtr0300009  NM_001144538   1812   589
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mia-PA   FBpp0088355   66.0   589   9.72     NP_649547   AAF52013 mia-PB   FBpp0289286   66.0   589   9.72     NP_001138010   ACL83469
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF1546 (IPR011442) Armadillo-like helical (IPR011989)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA941062   AE014297 AAF52013   AE014297 ACL83469   AW942715   AY060376 AAL25415   AY752878 AAV33343   BF487775   BI142021   BI635783   BT001890 AAN71679   BT029284   BT132955   CL528068   EC087874   GH942646   GH947217   GH947454   GH949219   GH951439   GH963041   GH964116   GH965245   GH965456   GH968258   GH973426   GH981856   GH981888
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VND0 Q8IG94 Q95T18 Q5UEC4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
male germ cell nucleus inferred from direct assay (Chen et al., 2005) nucleolus inferred from direct assay (Chen et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult midgut. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    30.95 Larval Midgut    78.3 Larval Hindgut    38.1 Larval Malpighian Tubules    22.3 Larval Fat Body    37.4 Larval Salivary Gland    24.1 Larval Trachea    37.65 Larval Carcass    19.3 Adult Head    20.2 Adult Eye    23.375 Adult Brain    16.3 Adult Thoracic-Abdominal Ganglion    29.5 Adult Crop    20 Adult Midgut    161.6 Adult Hindgut    18.1 Adult Malpighian Tubules    16.8 Adult Fat Body    16.7 Adult Salivary Gland    39 Adult Heart    12.05 Adult VirginFemale Spermatheca    16 Adult InseminatedFemale Spermatheca    22.4 Adult Ovary    19.3 Adult Testis    73.8 Adult Male Accessory Gland    29.5 Adult Carcass    17.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    20.2   NA  Eye    23.375   NA  Brain    16.3   30.95  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    29.5   NA  Crop    20   78.3  Midgut    161.6   38.1  Hindgut    18.1   22.3  Malpighian Tubules    no informative data   37.4  Fat Body    16.7   24.1  Salivary Gland    39   NA  Heart    12.05   37.65  Trachea    NA   NA  VirginFemale Spermatheca    16   NA  InseminatedFemale Spermatheca    22.4   NA  Ovary    19.3   NA  Testis    73.8   NA  Male Accessory Gland    29.5   19.3  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    4 central nervous system, pupae P8    2 head, virgin 1-day female    1 head, virgin 4-day female    1 head, virgin 20-day female    2 head, mated 1-day female    1 head, mated 4-day female    2 head, mated 20-day female    2 head, mated 1-day male    2 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    0 fat body, white prepupae    4 fat body, pupae P8    8 carcass, larvae L3 wandering    3 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    3 ovary, mated 4-day female    3 testis, mated 4-day male    22 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of very low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    4 embryo 04-06hr    6 embryo 06-08hr    8 embryo 08-10hr    5 embryo 10-12hr    2 embryo 12-14hr    2 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    1 embryo 20-22hr    1 embryo 22-24hr    1 larva L1    2 larva L2    3 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    3 white prepupae new    3 white prepupae 12hr    6 white prepupae 24hr    5 pupae 2d postWPP    3 pupae 3d postWPP    3 pupae 4d postWPP    3 adult male 01day    4 adult male 05day    5 adult male 30day    5 adult female 01day    2 adult female 05day    1 adult female 30day    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2    28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2  (28) wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2    28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2    28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2    28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2  28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1  26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2    28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    20 Schneider line 2 Sg4    18 embryonic 1182-4H    11 embryonic GM2    21 embryonic Kc167    12 embryonic S1    11 embryonic S3    20 leg disc CME L1    17 wing disc CME-W2    28 wing disc ML-DmD8    14 wing disc ML-DmD9    17 wing disc ML-DmD16-c3    11 wing disc ML-DmD21    11 wing disc ML-DmD32    13 haltere disc ML-DmD17-c3    16 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    20 mixed discs ML-DmD4-c1    13 CNS ML-DmBG1-c1    26 CNS ML-DmBG2-c2    18 tumorous blood cells mbn2    18 ovary fGS/OSS    2 ovary OSC    6 ovary OSS    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    1 cold shock, 4-day adult    1 heat shock, 4-day adult    3 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    1 Copper 15 mM 48 hrs, 4-day adult    1 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    2 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    2 Caffeine 25 mg/ml 48 hrs, 4-day adult    1 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    4 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_21 mE1_20_mRNA_expression_cluster_06
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014342 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10390-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable miaEY07883 viable, with Scer\GAL4pnr-MD237 miaGD4141 viable (with mia1) miaz-3348 viable (with miaz-3348) mia1 Sterility Allele fertile miaEY07883 fertile (with mia1) miaz-3348 fertile (with miaz-3348) mia1 male sterile | recessive mia1 Phenotype manifest in Allele spermatocyte mia1 testis mia1
Classical Alleles ( 6 )
For All Classical Alleles Show
Allele of mia Class Mutagen Stocks Known lesion miaB560 1 -- miaEY07883 P-element activity 1 -- miaLT P-element activity 1 Yes mia1 ethyl methanesulfonate 0 Yes miaunspecified 0 -- miaz-3348 ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mia Class Mutagen Stocks Known lesion miaGD4141 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes miaKK107112 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes miaNIG.10390R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Partially disrupted in	Df(3R)LT (O'Connor and Tanini, 2009.6.24)
Disrupted in	Df(3R)Exel6144 (Ryder, 2004.4.26)
Not disrupted in	Df(3L)Pc-MK (Lin, 1995.4.19, Lin et al., 1996) Df(3L)ri-79c (Lin, 1995.4.19, Lin et al., 1996) Df(3R)2-2 (Lin, 1995.4.19, Lin et al., 1996) Df(3R)Tpl10 (Lin, 1995.4.19, Lin et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4141} NA P{KK107112} NA P{NIG.10390R} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}miaEY07883 NA miscellaneous insertions P{3'EPgy2}LT NA PBac{5HPw+}miaB560 NA
Gene Ontology: Function, Process & Cellular Component ( 11 unique terms )
Terms Based on Experimental Evidence ( 6 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
male meiosis I inferred from mutant phenotype (Lin et al., 1996) regulation of gene expression inferred from mutant phenotype (White-Cooper et al., 1998) spermatid differentiation inferred from mutant phenotype (White-Cooper et al., 1998) spermatogenesis inferred from mutant phenotype (Lin et al., 1996)
Cellular Component
CV term Evidence References
male germ cell nucleus inferred from direct assay (Chen et al., 2005) nucleolus inferred from direct assay (Chen et al., 2005)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
meiosis traceable author statement (Fuller, 1998) regulation of sequence-specific DNA binding transcription factor activity inferred from electronic annotation with InterPro:IPR011442 (FlyBase Curators et al., 2004-) spermatid development traceable author statement (Fuller, 1998) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity with human TAF6L AND inferred from sequence or structural similarity with Saccharomyces TAF60 (Aoyagi and Wassarman, 2000)
Cellular Component
CV term Evidence References
transcription factor TFIID complex inferred from sequence or structural similarity with human TAF6L AND inferred from sequence or structural similarity with Saccharomyces TAF60 (Aoyagi and Wassarman, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mia allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0014342 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40667
Orthologs
OrthoDB Orthologs (17) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6N317N Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search EOG65QW69
Orthologs in Drosophila Species (EOG6N317N)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\mia     Drosophila simulans   Dsim\GD19609 Y   Drosophila sechellia   Dsec\GM10624 Y   Drosophila sechellia   Dsec\GM23247     Drosophila erecta   Dere\GG10968 Y   Drosophila yakuba   Dyak\GE24166 Y   Drosophila ananassae   Dana\GF16042 Y   Drosophila willistoni   Dwil\GK13782 Y   Drosophila virilis   Dvir\GJ24553 Y   Drosophila mojavensis   Dmoj\GI24488 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (EOG65QW69)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\taf-6.1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028809gn Y Danio rerio Zebrafish  Drer\taf6 Y Xenopus tropicalis Western clawed frog  Xtro\taf6 Y Mus musculus House mouse  Mmus\Taf6 Y Rattus norvegicus Norway rat  Rnor\Taf6 Y Homo sapiens Human  Hsap\TAF6 Y
Human Orthologs (1)
Gene OMIM HGNC Hsap\TAF6
AAA Orthologs (9) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19609 Drosophila sechellia Dsec\GM10624 Drosophila erecta Dere\GG10968 Drosophila yakuba Dyak\GE24166 Drosophila ananassae Dana\GF16042 Drosophila willistoni Dwil\GK13782 Drosophila virilis Dvir\GJ24553 Drosophila mojavensis Dmoj\GI24488 Drosophila grimshawi Dgri\GH17238
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	16865 y1 w67c23; P{EPgy2}miaEY07883 27595 y1 w*; Df(3R)LT, miaLT PcmtLT 16244 y1 w1118; PBac{5HPw+}miaB560/TM3, Sb1 Ser1
VDRC	v100313 P{KK107112}VIE-260B v37563 w1118; P{GD4141}v37563/CyO
Genomic Clones ( 1 )
	BACR24O24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 20 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD25013 SD16013
Other clones	FI17401 RT01020
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT22828 SD17264
Other clones	104186 RP004161087 RP004187319 RP004209461 RP004211974 RP004226088 RP004227295 RP004265187 RP004381877 RP004457210 RP004491806 RP004495514 RP004508084 RP004518985
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0014342 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40667
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Taf60-2 CG10390 (Aoyagi and Wassarman, 2000)
Source for database merge of	Source for merge of: mia Taf6L (Hiller et al., 2004)
Additional comments
Other Comments
	can, mia and sa are required for accumulation of tweT:Ecol\lacZ transgene in late primary spermatocytes. (White-Cooper et al., 1998) Wild type function of aly, can, mia and sa is required for cell-cycle progression through the G2/M transition of meiosis I in males and for onset of spermatid differentiation. The phenotype of these meiotic arrest mutants is strikingly similar to the histopathological features of meiosis I maturation arrest infertility in human males, suggesting that the control point may be conserved from flies to man. (Lin et al., 1996) mia is required for both the progression of the male meiotic cell cycle and the onset of postmeiotic differentiation. (Lin et al., 1995) Mutations in mia cause a complete arrest of spermatogenesis at the G2/M transition to meiosis I, similar to aly, can, and sa. (Lin, 1995.4.19)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_141290 NM_001144538 RefSeq (Proteins) • NP_649547 NP_001138010 DDBJ / EMBL / GenBank • AA941062 AAF52013 AAL25415 AAN71679 AAV33343 ACL83469 AW942715 AY060376 AY752878 BI142021 BT001890 CL528068 Entrez Gene - A searchable database of RefSeq genes. • 40667 UniProtKB/TrEMBL • Q5UEC4 Q8IG94 Q9VND0 Q95T18
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Domain of unknown function DUF1546 (IPR011442) Armadillo-like helical (IPR011989)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0014342 DRSC - Results from RNAi screens. • FBgn0014342 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0014342 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10390-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0014342 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40667 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 40667 modMine - Data generated by the modENCODE project. • FBgn0014342
Synonyms & Secondary IDs ( 21 )
Reported As
Symbol Synonym	CG10390 (Labourier et al., 2002, Keleman et al., 2009.8.5, Aoyagi and Wassarman, 2001) dmTAF6b (Hochheimer and Tjian, 2003) dTAF6L (Hiller et al., 2004) dTAFII60 (Hochheimer and Tjian, 2003) dTAFII60-2 (Veenstra and Wolffe, 2001) mia (Ceulemans, 2006, Mikhaylova et al., 2006, Arya et al., 2006, Jiang et al., 2007, Chen et al., 2005, Chen et al., 2005, Metcalf and Wassarman, 2007, Yakoby et al., 2008, Moon et al., 2011, O'Connor and Tanini, 2009.6.24, Weake et al., 2009, Di Cara et al., 2010, Janic et al., 2010, Japanese National Institute of Genetics, 2012.5.21) Mia (Davis et al., 2011) ms(3)570   TAF (Mueller and Tora, 2004) Taf6L (Bloomington Drosophila Stock Center, 2004.11.7, Spedale et al., 2012) TAF6L (Tora, 2002, Weake et al., 2009) Taf60-2 (Orian et al., 2003, ) TAF60-2 (Aoyagi and Wassarman, 2000) TAFII (Arnosti, 2002) TAFII60-2 (Aoyagi and Wassarman, 2001) TFIID (Butler and Kadonaga, 2002, Hochheimer et al., 2002, Gu et al., 2002, Stathopoulos and Levine, 2002, Mizuguchi et al., 2001)
Name Synonym	meiosis I arrest (Moon et al., 2011, Weake et al., 2009, Davis et al., 2011) Meiosis I arrest (Metcalf and Wassarman, 2007) TAF6-like (Hiller et al., 2004.9.14) TBP-associated factor 6-like   TBP-associated factor 60kD-2
Secondary FlyBase IDs
	FBgn0037337
References ( 67 )
Generate a list of	All references relating to this gene ( 67 )
List References by type	Research paper  ( 27 ) Supplementary material  ( 4 ) Review  ( 10 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 10 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Davis et al., 2011, BMC Dev. Biol. 11: 66 Identification of common and cell type specific LXXLL motif EcR cofactors using a bioinformatics refined candidate RNAi screen in Drosophila melanogaster cell lines. [FBrf0216830] Moon et al., 2011, Development 138(17): 3835--3845 The THO complex is required for nucleolar integrity in Drosophila spermatocytes. [FBrf0214600] Show all research papers ...
Recent reviews ( 1 )
	Spedale et al., 2012, Genes Dev. 26(6): 527--541 ATAC-king the complexity of SAGA during evolution. [FBrf0219841] Show all reviews ...