FlyBase Gene Report: Dmel\Mcm2

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

(UniProt, P49735)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Mcm2 or the JBrowse view of Dmel\Mcm2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Mcm2-RA

FBtr0081827

NM_057773

2898

887

Additional Transcript Data and Comments

Reported size (kB)

2.8 (compiled cDNA)

(Treisman et al., 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Mcm2-PA

FBpp0081317

100.4

887

4.74

NP_477121

AAF54207

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

887 (aa)

(Treisman et al., 1995)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.

(UniProt, P49735)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mcm2_1

Linkouts

Gene Ontology (9 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

contributes_to 3'-5' DNA helicase activity

inferred from direct assay

(Moyer et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008045, InterPro:IPR018525

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

DNA binding

inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008045, InterPro:IPR018525

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

chromosome condensation

inferred from mutant phenotype

(Christensen and Tye, 2003)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

DNA replication

traceable author statement

(Lee and Orr-Weaver, 2003)

DNA replication initiation

inferred from electronic annotation with InterPro:IPR008045

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

negative regulation of DNA helicase activity

inferred from electronic annotation with InterPro:IPR008045

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

MCM complex

inferred from electronic annotation with InterPro:IPR008045

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

nucleus

inferred from electronic annotation with InterPro:IPR008045

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

presumptive embryonic/larval peripheral nervous system

(Treisman et al., 1995)

central nervous system

(Treisman et al., 1995)

Malpighian tubule

(Treisman et al., 1995)

embryonic/larval digestive system

(Treisman et al., 1995)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4

organism | ubiquitous

(Treisman et al., 1995)

embryonic stage 4 -- 6

mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 8

organism | ubiquitous

(Treisman et al., 1995)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

antennal primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

dorsal sensory complex primordium

(Fisher et al., 2012)

labial sensory complex primordium

(Fisher et al., 2012)

maxillary sensory complex primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

ventral sensory complex anlage

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic central brain neuron

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

lateral cord

(Fisher et al., 2012)

lateral cord neuron

(Fisher et al., 2012)

sensory nervous system primordium

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Mcm2 transcripts are maternally contributed to the embryo and disappear at cellularization. They are expressed uniformly at germ band extension and are subsequently restricted to a pattern that includes continuous expression in the CNS and transient expression in a number of other tissues, including the Malpighian tubules and the gut.

(Treisman et al., 1995)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{PZ}Mcm2^rL074

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

photoreceptor cell R8 | presumptive

(Treisman and Rubin, 1996)

eye disc | restricted

(Treisman and Rubin, 1996)

wandering third instar larval stage

embryonic/larval brain

(Meister and Braun, 1995.10)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Mcm2

View exonic expression by tissue for Dmel\Mcm2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014861

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0014861

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG7538-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7538

Flygut - An atlas of the Drosophila adult midgut

FBgn0014861

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Mcm2

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 2 )

For All Classical and Insertion Alleles Show

Allele of Mcm2

Class

Mutagen

Associated Insertion

Stocks

Known lesion

Mcm2^rL074

P-element activity

P{PZ}Mcm2^rL074

Yes

Mcm2^MI06343

Mi{MIC}Mcm2^MI06343

Yes

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Mcm2

Allele of Mcm2

Mutagen

Associated Transgenic Construct

Stocks

in vitro construct - regulatory fusion

Mcm2^{UAS.ORF.Tag:HA}

M{UAS-Mcm2.ORF.3xHA}

Mcm2^UAS.ORF

in vitro construct - regulatory fusion

M{UAS-Mcm2.ORF}

Transgenic constructs containing regulatory region of Mcm2

Deletions and Duplications ( 3 )

Disrupted in

Df(3R)ED5296

(Ryder and Roote, 2004.11.10)

Df(3R)Exel6147

(Ryder, 2004.4.26)

Df(3R)p712

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | somatic clone | third instar larval stage

Mcm2^rL074

lethal

Mcm2^GD4467

lethal | recessive

Mcm2^rL074

viable, with Scer\GAL4^Mef2.PR

Mcm2^GD4467

viable, with Scer\GAL4^pnr-MD237

Mcm2^GD4467

viable, with Scer\GAL4^tin.CΔ4

Mcm2^GD4467

Other Phenotypes

Allele

decreased occurrence of cell division | somatic clone | third instar larval stage

Mcm2^rL074

neuroanatomy defective | larval stage

Mcm2^GD4467

size defective | third instar larval stage

Mcm2^rL074

some die during immature adult stage | recessive

Mcm2^rL074

visible, with Scer\GAL4^pnr-MD237

Mcm2^GD4467

Phenotype manifest in

Allele

central nervous system

Mcm2^rL074

chaeta, with Scer\GAL4^pnr-MD237

Mcm2^GD4467

embryonic/larval neuroblast | larval stage

Mcm2^GD4467

ganglion mother cell | larval stage

Mcm2^GD4467

imaginal disc

Mcm2^rL074

trichogen cell, with Scer\GAL4^pnr-MD237

Mcm2^GD4467

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MCM2

12 of 15

Yes

Hsap\MCM9

1 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Mcm2

12 of 15

Yes

Mmus\Mcm9

1 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Mcm2

12 of 13

Yes

Rnor\Mcm9

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\mcm2

11 of 12

Yes

Xtro\mcm9

1 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\mcm2

9 of 15

Yes

Drer\mcm9

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\mcm-2

14 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\MCM2

9 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MCM2

14 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\mcm2

12 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091902C3 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Mcm2

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA20424

Drosophila persimilis

Dper\GL23360

Drosophila willistoni

Dwil\GK11732

Drosophila virilis

Dvir\GJ24206

Drosophila mojavensis

Dmoj\GI22091

Drosophila grimshawi

Dgri\GH14181

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501GI )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17011505

Glossina morsitans

Tsetse fly

Gmor\GMOY006553

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10787

Mayetiola destructor

Hessian fly

Mdes\Mdes001391

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007007

Anopheles darlingi

American malaria mosquito

Adar\ADAC004997

Anopheles gambiae

Malaria mosquito

Agam\AGAP012691

Anopheles gambiae

Malaria mosquito

Agam\AGAP004275

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ002555

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W019D )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA008625

Danaus plexippus

Monarch butterfly

Dple\DPOGS207360-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008863

Apis florea

Little honeybee

Aflo\2253639892

Apis mellifera

Western honey bee

Amel\GB49556

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP24095

Bombus terrestris

Buff-tailed bumblebee

Bter\15215911

Linepithema humile

Argentine ant

Lhum\LH13561

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0031c1

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010488

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07124

Tribolium castaneum

Red flour beetle

Tcas\TC011437

Tribolium castaneum

Red flour beetle

Tcas\TC005192

Pediculus humanus

Human body louse

Phum\PHUM492170

Rhodnius prolixus

Kissing bug

Rpro\RPRC013172

Cimex lectularius

Bed bug

Clec\CLEC007367

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003255

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_02816

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X017H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008214

Ixodes scapularis

Black-legged tick

Isca\ISCW016696

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59250.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur13g00670

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_306072

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G026H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Mcm2_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Mcm2

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000007846

Ciona intestinalis

Vase tunicate

Cint\LOC101243119

Gallus gallus

Domestic chicken

Ggal\MCM2

Human Disease Model Data

FlyBase Human Disease Model Reports

Alleles Reported to Model Human Disease (Disease Ontology)

Download

Models ( 0 )

Allele

Disease

Evidence

References

Interactions ( 0 )

Allele

Disease

Interaction

References

Comments ( 0 )

Human Orthologs (via DIOPT v7.1)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

Complementation?

Transgene?

MCM2; minichromosome maintenance complex component 2

12 of 15

116945

DEAFNESS, AUTOSOMAL DOMINANT 70; DFNA70

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please look at the Interaction Group reports for full details of the physical interactions

protein-protein

Interacting group

Assay

References

Mcm2 - CDC45L

anti bait coimmunoprecipitation, mass spectrometry studies of complexes, anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, electron microscopy, cosedimentation, western blot

(Costa et al., 2014, Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006, Loebel et al., 2000)

Mcm2 - Cdc7

enzymatic study, autoradiography

(Stephenson et al., 2015)

Mcm2 - Ctf4

two hybrid

(Gosnell and Christensen, 2011)

Mcm2 - Mcm10

two hybrid

(Apger et al., 2010)

Mcm2 - Mcm3

cosedimentation, electron microscopy, pull down, western blot, anti tag coimmunoprecipitation

(Costa et al., 2014, Costa et al., 2011)

Mcm2 - Mcm5

anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Moyer et al., 2006, Loebel et al., 2000)

Mcm2 - Mcm6

cosedimentation, electron microscopy, experimental knowledge based

(Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)

Mcm2 - Mcm7

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm2 - Psf1

cosedimentation, electron microscopy

(Costa et al., 2011)

Mcm2 - Psf2

anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy

(Costa et al., 2011, Moyer et al., 2006)

Mcm2 - Psf3

cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot

(Costa et al., 2011, Moyer et al., 2006)

Mcm2 - Sld5

cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot

(Costa et al., 2011, Moyer et al., 2006)

Mcm2 - dpa

cosedimentation, electron microscopy

(Costa et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

E2f2

suppressible

Mcm2

(Cayirlioglu et al., 2003)

External Data

Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.

(UniProt, P49735 )

Linkouts

BioGRID - A database of protein and genetic interactions.

66154

DroID - A comprehensive database of gene and protein interactions.

FBgn0014861

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40973

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

DNA replication

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-48

Cytogenetic map

84F6-84F6

Sequence location

3R:8,264,970..8,267,924 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

84F6-84F6

Limits computationally determined from genome sequence between P{PZ}grn⁰⁵⁹³⁰ and P{PZ}Mcm2^rL074

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

84F6-84F7

(Treisman, 1995.9.29, BDGP Project Members, 1994-1999)

84F6-84F7

(determined by in situ hybridisation)

(Spradling et al., 1999)

Experimentally Determined Recombination Data

Location

3-48

(FlyBase, 2016)

3-44.8

(Comeron et al., 2012)

(Treisman, 1995.9.29)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

80894

y¹, v¹; P{TKO.GS04798}attP40

43056

y¹ w^*; Mi{MIC}Mcm2^MI06343

35771

y¹ sc^* v¹; P{TRiP.HMS01520}attP2

FlyORF

F001810

M{UAS-Mcm2.ORF}ZH-86Fb

F001143

M{UAS-Mcm2.ORF.3xHA}ZH-86Fb

VDRC

v103619

P{KK101748}VIE-260B

v10967

w¹¹¹⁸; P{GD4467}v10967/TM3

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (50)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD47441

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0014861

cDNA Clones, End Sequenced (ESTs)

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0014861

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40973

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Mcm2 CG7538

(Zhao, 1999.11)

Source for database merge of

Additional comments

Other Comments

The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

(Moyer et al., 2006)

CycE protein can drive chromosome association of Mcm2 in polytene nuclei and DNA synthesis erases this association.

(Su and O'Farrell, 1998)

Physical interactions exist between three MCM proteins, dpa, Mcm2 and Mcm5. The three proteins reside predominantly in stable multi-protein complexes of about 600kD. Coimmunoprecipitation experiments indicate there are at least two distinct 600kD complexes, one of which includes Mcm5 and the other dpa.

(Su et al., 1996)

For the yeast genes MCM stands for 'minichromosome maintenance'.

(Treisman et al., 1995)

Mcm2 plays a role in the regulation of DNA replication analogous to that of its yeast counterpart (MCM2).

(Treisman et al., 1995)

Origin and Etymology

Discoverer

G. Rubin.

Etymology

Identification

External Crossreferences and Linkouts ( 47 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

40973

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P49735

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014861

InterPro - A database of protein families, domains and functional sites

Linkouts

BioGRID - A database of protein and genetic interactions.

66154

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones

FBgn0014861

DroID - A comprehensive database of gene and protein interactions.

FBgn0014861

DRSC - Results frm RNAi screens

FBgn0014861

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Mcm2_1

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0014861

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG7538-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0014861

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG7538

Flygut - An atlas of the Drosophila adult midgut

FBgn0014861

FlyMine - An integrated database for Drosophila genomics

FBgn0014861

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

40973

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC005192

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

40973

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG7538

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

DNA replication

MIST (protein-protein) - An integrated Molecular Interaction Database

Mcm2

modMine - A data warehouse for the modENCODE project

FBgn0014861

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

CG7538

(Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5)

DmMCM2

(Kearsey and Labib, 1998, Ohno et al., 1998, Su and O'Farrell, 1998, Su and O'Farrell, 1997, Su et al., 1997, Su et al., 1996, Treisman et al., 1995)

DmMcm2

(Loebel et al., 2000)

DmeMCM2

(Blanton et al., 2005)

MCM2

(Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Apger et al., 2010, Ilves et al., 2010, Lemos et al., 2010, Moshkin et al., 2009, Crevel et al., 2007, Hong et al., 2007, Lee and Orr-Weaver, 2003, Claycomb et al., 2002, Crevel et al., 2001, Feger, 1999, Treisman, 1995.9.29)

MCM2-7

(Kim and Orr-Weaver, 2011, Macalpine et al., 2010)

MCM2_DROME

(Storm and Sonnhammer, 2003)

McM2

(De Lucia et al., 2005)

Mcm2-7

(Kohzaki and Murakami, 2018)

PCR3

(Su et al., 1997)

dMCM2

(Burke et al., 2001)

dMcm2

(Sahashi et al., 2013)

l(3)rL074

mcm2

(Haines and Eisen, 2018)

mcm2-7

(Powell et al., 2015)

Name Synonyms

Minichromosome maintenance 2

(Dequéant et al., 2015, Fernandez-Costa and Artero, 2010)

Secondary FlyBase IDs

FBgn0011511

Datasets (1)

Study focus (1)

Experimental Role

Project

Project Type

Title

bait_protein

modENCODE_replication_factor_ChIP

genome binding

Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.

References (120)

The following external sites may use different assemblies or annotations than FlyBase.

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