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FB2021_04, released August 17, 2021

Gene: Dmel\Mcm2

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General Information
Symbol
Dmel\Mcm2
Species
D. melanogaster
Name
Minichromosome maintenance 2
Annotation Symbol
CG7538
Feature Type
protein_coding_gene
FlyBase ID
FBgn0014861
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation. (UniProt, P49735)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

DmMCM2, MCM2-7

Key Links
Genomic Location
Cytogenetic map
84F6-84F6
Sequence location
3R:8,264,970..8,267,924 [+]
Recombination map
3-48
RefSeq locus
NT_033777 REGION:8264970..8267924
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (14 terms)
Molecular Function (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
contributes_to 3'-5' DNA helicase activity
inferred from direct assay
(Moyer et al., 2006)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008045, InterPro:IPR018525
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables single-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
contributes_to single-stranded DNA helicase activity
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (7 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in chromosome condensation
inferred from mutant phenotype
(Christensen and Tye, 2003)
Terms Based on Predictions or Assertions (6 terms)
CV Term
Evidence
References
involved_in DNA replication
traceable author statement
(Lee and Orr-Weaver, 2003)
involved_in DNA unwinding involved in DNA replication
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in double-strand break repair via break-induced replication
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in mitotic DNA replication initiation
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in negative regulation of DNA helicase activity
inferred from electronic annotation with InterPro:IPR008045
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
involved_in pre-replicative complex assembly involved in nuclear cell cycle DNA replication
inferred from biological aspect of ancestor with PANTHER:PTN000179907
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of MCM complex
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000179711
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the MCM family. (P49735)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
MCM2-7 COMPLEX -
The heterohexameric MCM2-7 complex is the helicase motor of the CMG (Cdc45-MCM-GINS) replicative helicase of eukaryotes. The six MCM subunits are related to one another and are members of the AAA+ (ATPases associated with diverse cellular activities) superfamily. Hydrolysis of ATP by the individual MCM subunits catalyses the conformational changes that drive unwinding of the DNA duplex. However, the eukaryotic MCM complex alone has no or little helicase activity, requiring the other components of the CMG complex (Cdc45 and the GINS complex) for activation. (Adapted from PMID:23412083.)
CMG COMPLEX -
The CMG (Cdc45-MCM-GINS) complex is the eukaryotic replicative helicase, the enzyme that unwinds double-stranded DNA at replication forks. It is a macromolecular assembly of three replication factors: the Cdc45 protein, and the MCM and GINS complexes. All three components are essential for CMG function, with the MCM2-7 complex functioning as the molecular motor that harnesses the energy of ATP hydrolysis to catalyse strand separation. (Adapted from PMID:23412083.)
Protein Function (UniProtKB)
Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.
(UniProt, P49735)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Mcm2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Mcm2-RA
FBtr0081827
NM_057773
2898
887
Additional Transcript Data and Comments
Reported size (kB)

2.8 (compiled cDNA)

(Treisman et al., 1995)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Mcm2-PA
FBpp0081317
100.4
887
4.74
NP_477121
AAF54207
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

887 (aa)

(Treisman et al., 1995)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.

(UniProt, P49735)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Mcm2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
presumptive embryonic/larval peripheral nervous system
(Treisman et al., 1995)
central nervous system
(Treisman et al., 1995)
Malpighian tubule
(Treisman et al., 1995)
embryonic/larval digestive system
(Treisman et al., 1995)
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4
organism | ubiquitous
(Treisman et al., 1995)
embryonic stage 4 -- 6
mesoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
posterior ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 8
organism | ubiquitous
(Treisman et al., 1995)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral epidermis primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
antennal primordium
(Fisher et al., 2012)
central brain primordium
(Fisher et al., 2012)
dorsal sensory complex primordium
(Fisher et al., 2012)
labial sensory complex primordium
(Fisher et al., 2012)
maxillary sensory complex primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
ventral sensory complex anlage
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
lateral cord
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data

Mcm2 transcripts are maternally contributed to the embryo and disappear at cellularization. They are expressed uniformly at germ band extension and are subsequently restricted to a pattern that includes continuous expression in the CNS and transient expression in a number of other tissues, including the Malpighian tubules and the gut.

(Treisman et al., 1995)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of CMG complex
inferred from direct assay
(Ilves et al., 2010, Moyer et al., 2006)
part_of MCM complex
inferred from direct assay
(Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Mcm2 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Mcm2
Transgenic constructs containing regulatory region of Mcm2
Aberrations (Deficiencies and Duplications) ( 3 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (9)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\MCM2
13 of 15
Yes
Yes
Hsap\MCM3
2 of 15
No
No
Hsap\MCM4
2 of 15
No
No
Hsap\MCM5
2 of 15
No
No
Hsap\MCM6
2 of 15
No
No
Hsap\MCM7
2 of 15
No
No
Hsap\MCM8
2 of 15
No
No
Hsap\MCM9
2 of 15
No
Yes
Hsap\MCMDC2
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Mcm2
14 of 15
Yes
Yes
Mmus\Mcm4
2 of 15
No
No
Mmus\Mcm5
2 of 15
No
No
Mmus\Mcm6
2 of 15
No
No
Mmus\Mcm7
2 of 15
No
No
Mmus\Mcm8
2 of 15
No
No
Mmus\Mcm9
2 of 15
No
Yes
Mmus\Mcm3
1 of 15
No
No
Rattus norvegicus (Norway rat) (10)
Rnor\Mcm2
13 of 13
Yes
Yes
Rnor\Mcm3
2 of 13
No
No
Rnor\Mcm4
2 of 13
No
No
Rnor\Mcm5
2 of 13
No
No
Rnor\Mcm6
2 of 13
No
No
Rnor\Mcm7
2 of 13
No
No
Rnor\Mcm8
2 of 13
No
No
Rnor\Mcm9
2 of 13
No
Yes
Rnor\Asf1a
1 of 13
No
No
Rnor\Mcmdc2
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (10)
Xtro\mcm2
13 of 12
Yes
Yes
Xtro\mcm3
2 of 12
No
No
Xtro\mcm3
2 of 12
No
No
Xtro\mcm4
2 of 12
No
No
Xtro\mcm5
2 of 12
No
No
Xtro\mcm6.2
2 of 12
No
No
Xtro\mcm6
2 of 12
No
No
Xtro\mcm7
2 of 12
No
No
Xtro\mcm8
2 of 12
No
No
Xtro\mcm9
2 of 12
No
Yes
Danio rerio (Zebrafish) (11)
Drer\mcm2
12 of 15
Yes
Yes
Drer\mcm3
2 of 15
No
No
Drer\mcm3l
2 of 15
No
No
Drer\mcm4
2 of 15
No
No
Drer\mcm5
2 of 15
No
No
Drer\mcm6
2 of 15
No
No
Drer\mcm6l
2 of 15
No
No
Drer\mcm7
2 of 15
No
No
Drer\mcm8
2 of 15
No
No
Drer\mcm9
2 of 15
No
Yes
Drer\mcmdc2
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\mcm-2
14 of 15
Yes
Yes
Cele\mcm-3
2 of 15
No
No
Cele\mcm-6
2 of 15
No
No
Cele\mcm-7
2 of 15
No
No
Cele\mcm-4
1 of 15
No
No
Cele\mcm-5
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (8)
Atha\MCM2
9 of 9
Yes
Yes
Atha\MCM3
1 of 9
No
No
Atha\MCM4
1 of 9
No
No
Atha\MCM5
1 of 9
No
No
Atha\MCM6
1 of 9
No
No
Atha\MCM8
1 of 9
No
No
Atha\MCM9
1 of 9
No
Yes
Atha\PRL
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (6)
Scer\MCM2
13 of 15
Yes
Yes
Scer\MCM3
1 of 15
No
No
Scer\MCM4
1 of 15
No
No
Scer\MCM5
1 of 15
No
No
Scer\MCM6
1 of 15
No
No
Scer\MCM7
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\mcm2
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091902C3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00010646
Drosophila simulans
Dsim\GD18528
Drosophila sechellia
Dsec\GM23713
Drosophila erecta
Dere\GG24143
Drosophila yakuba
Dyak\GE25859
Drosophila ananassae
Dana\GF18817
Drosophila pseudoobscura pseudoobscura
Dpse\GA20424
Drosophila persimilis
Dper\GL23360
Drosophila willistoni
Dwil\GK11732
Drosophila virilis
Dvir\GJ24206
Drosophila mojavensis
Dmoj\GI22091
Drosophila grimshawi
Dgri\GH14181
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501GI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011505
Glossina morsitans
Tsetse fly
Gmor\GMOY006553
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10787
Mayetiola destructor
Hessian fly
Mdes\Mdes001391
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007007
Anopheles darlingi
American malaria mosquito
Adar\ADAC004997
Anopheles gambiae
Malaria mosquito
Agam\AGAP012691
Anopheles gambiae
Malaria mosquito
Agam\AGAP004275
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ002555
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W019D )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA008625
Danaus plexippus
Monarch butterfly
Dple\DPOGS207360-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL008863
Apis florea
Little honeybee
Aflo\2253639892
Apis mellifera
Western honey bee
Amel\GB49556
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP24095
Bombus terrestris
Buff-tailed bumblebee
Bter\15215911
Linepithema humile
Argentine ant
Lhum\LH13561
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0031c1
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG010488
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07124
Tribolium castaneum
Red flour beetle
Tcas\TC011437
Tribolium castaneum
Red flour beetle
Tcas\TC005192
Pediculus humanus
Human body louse
Phum\PHUM492170
Rhodnius prolixus
Kissing bug
Rpro\RPRC013172
Cimex lectularius
Bed bug
Clec\CLEC007367
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI003255
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_02816
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X017H )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR008214
Ixodes scapularis
Black-legged tick
Isca\ISCW016696
Stegodyphus mimosarum
African social velvet spider
Smim\KFM59250.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur13g00670
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_306072
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G026H )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mcm2_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Mcm2
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000007846
Ciona intestinalis
Vase tunicate
Cint\LOC101243119
Gallus gallus
Domestic chicken
Ggal\MCM2
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
dpa
4 of 10
Mcm3
4 of 10
Mcm5
4 of 10
Mcm6
4 of 10
Mcm7
4 of 10
rec
4 of 10
mei-218
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    MCM2; minichromosome maintenance complex component 2
    13 of 15
    116945
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser
    View in Interactions Browser
    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Mcm2 - Cdc45
    anti tag coimmunoprecipitation, molecular weight estimation by silver staining, anti tag western blot, anti bait coimmunoprecipitation, mass spectrometry studies of complexes, cosedimentation, electron microscopy, western blot
    (Costa et al., 2014, Costa et al., 2011, Ilves et al., 2010, Moyer et al., 2006, Loebel et al., 2000)
    Mcm2 - Cdc7
    enzymatic study, autoradiography
    (Stephenson et al., 2015)
    Mcm2 - Ctf4
    two hybrid
    (Gosnell and Christensen, 2011)
    Mcm2 - Mcm10
    two hybrid
    (Apger et al., 2010)
    Mcm2 - Mcm3
    cosedimentation, electron microscopy, pull down, western blot, anti tag coimmunoprecipitation
    (Costa et al., 2014, Costa et al., 2011)
    Mcm2 - Mcm5
    anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy, experimental knowledge based
    (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011, Moyer et al., 2006, Loebel et al., 2000)
    Mcm2 - Mcm6
    electron microscopy, experimental knowledge based, cosedimentation
    (Costa et al., 2014, Costa et al., 2011, Guruharsha et al., 2011)
    Mcm2 - Mcm7
    cosedimentation, electron microscopy
    (Costa et al., 2011)
    Mcm2 - Psf1
    cosedimentation, electron microscopy
    (Costa et al., 2011)
    Mcm2 - Psf2
    anti bait coimmunoprecipitation, western blot, cosedimentation, electron microscopy
    (Costa et al., 2011, Moyer et al., 2006)
    Mcm2 - Psf3
    cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot
    (Costa et al., 2011, Moyer et al., 2006)
    Mcm2 - Sld5
    cosedimentation, electron microscopy, anti bait coimmunoprecipitation, western blot
    (Costa et al., 2011, Moyer et al., 2006)
    Mcm2 - dpa
    cosedimentation, electron microscopy
    (Costa et al., 2011)
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    E2f2
    suppressible
    Mcm2
    (Cayirlioglu et al., 2003)
    External Data
    Subunit Structure (UniProtKB)
    Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable). Interacts with Mcm10.
    (UniProt, P49735 )
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    3R
    Recombination map
    3-48
    Cytogenetic map
    84F6-84F6
    Sequence location
    3R:8,264,970..8,267,924 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    84F6-84F6
    Limits computationally determined from genome sequence between P{PZ}grn05930 and P{PZ}Mcm2rL074
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    84F6-84F7
    (Treisman, 1995.9.29, BDGP Project Members, 1994-1999)
    84F6-84F7
    (determined by in situ hybridisation)
    (Spradling et al., 1999)
    Experimentally Determined Recombination Data
    Location

    3-48

    (FlyBase, 2016)

    3-44.8

    (Comeron et al., 2012)

    3-

    (Treisman, 1995.9.29)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (7)
    Genomic Clones (14)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (50)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: Mcm2 CG7538

      (Zhao, 1999.11)
      Source for database merge of
      Additional comments
      Other Comments

      The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells.

      (Moyer et al., 2006)

      CycE protein can drive chromosome association of Mcm2 in polytene nuclei and DNA synthesis erases this association.

      (Su and O'Farrell, 1998)

      Physical interactions exist between three MCM proteins, dpa, Mcm2 and Mcm5. The three proteins reside predominantly in stable multi-protein complexes of about 600kD. Coimmunoprecipitation experiments indicate there are at least two distinct 600kD complexes, one of which includes Mcm5 and the other dpa.

      (Su et al., 1996)

      For the yeast genes MCM stands for 'minichromosome maintenance'.

      (Treisman et al., 1995)

      Mcm2 plays a role in the regulation of DNA replication analogous to that of its yeast counterpart (MCM2).

      (Treisman et al., 1995)
      Origin and Etymology
      Discoverer

      G. Rubin.

      Etymology
      Identification
      External Crossreferences and Linkouts ( 48 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DRscDB
      EMBL-EBI Single Cell Expression Atlas
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (17)
      Reported As
      Symbol Synonym
      CG7538
      (Hosono et al., 2015, Schertel et al., 2013, Ni et al., 2011.7.19, Keleman et al., 2009.8.5)
      DmMCM2
      (Kearsey and Labib, 1998, Ohno et al., 1998, Su and O'Farrell, 1998, Su and O'Farrell, 1997, Su et al., 1997, Su et al., 1996, Treisman et al., 1995)
      DmMcm2
      (Loebel et al., 2000)
      DmeMCM2
      (Blanton et al., 2005)
      MCM2
      (Wooten et al., 2020, Hua and Orr-Weaver, 2017, Bleichert et al., 2015, Crevel et al., 2011, Apger et al., 2010, Ilves et al., 2010, Lemos et al., 2010, Moshkin et al., 2009, Crevel et al., 2007, Hong et al., 2007, Lee and Orr-Weaver, 2003, Claycomb et al., 2002, Crevel et al., 2001, Feger, 1999, Treisman, 1995.9.29)
      MCM2-7
      (Kim and Orr-Weaver, 2011, Macalpine et al., 2010)
      MCM2_DROME
      (Storm and Sonnhammer, 2003)
      McM2
      (De Lucia et al., 2005)
      Mcm2
      (Sureka and Mishra, 2021, Kose et al., 2020, Williams et al., 2020, Burnham et al., 2019, Parker et al., 2019, Vorobyeva and Mazina, 2019, Kohzaki, 2018, Kohzaki et al., 2018, Sureka et al., 2018, Transgenic RNAi Project members, 2017-, Kong et al., 2016, Völker-Albert et al., 2016, Dequéant et al., 2015, Nikalayevich and Ohkura, 2015, Stephenson et al., 2015, Zirin et al., 2015, Costa et al., 2014, Korenjak et al., 2014, Liang et al., 2014, Luck et al., 2014, Yan et al., 2014, Gurudatta et al., 2013, Kwon et al., 2013, Moshkin et al., 2013, Schertel et al., 2013, Antao et al., 2012, Chen et al., 2012, Kalajdzic et al., 2012, Longworth et al., 2012, Costa et al., 2011, Gosnell and Christensen, 2011, Neumüller et al., 2011, Orihara-Ono et al., 2011, Frise et al., 2010, Maqbool et al., 2010, McElroy et al., 2008, Foglietti et al., 2006, Moyer et al., 2006, Loop et al., 2004, Cayirlioglu et al., 2003)
      Mcm2-7
      (Kohzaki and Murakami, 2018)
      PCR3
      (Su et al., 1997)
      dMCM2
      (Burke et al., 2001)
      dMcm2
      (Sahashi et al., 2013)
      l(3)rL074
      mcm2
      (Haines and Eisen, 2018)
      mcm2-7
      (Powell et al., 2015)
      Name Synonyms
      Minichromosome maintenance 2
      (Dequéant et al., 2015, Fernandez-Costa and Artero, 2010)
      Secondary FlyBase IDs
      • FBgn0011511
      Datasets (1)
      Study focus (1)
      Experimental Role
      Project
      Project Type
      Title
      • bait_protein
      modENCODE_replication_factor_ChIP
      Genome-wide localization of essential replication factors characterized by ChIP-Seq and ChIP-chip.
      References (134)