FlyBase Gene Report: Dmel\btn

General Information

Symbol

Dmel\btn

Species

D. melanogaster

Name

buttonless

Annotation Symbol

CG5264

Feature Type

protein_coding_gene

FlyBase ID

FBgn0014949

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Summary

buttonless (btn) encodes a protein that contributes to cuticle pigmentation. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

94B5-94B5

Sequence location

3R:22,586,149..22,586,625 [-]

Recombination map

3-75

RefSeq locus

NT_033777 REGION:22586149..22586625

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables DNA binding

inferred from electronic annotation with InterPro:IPR001356, InterPro:IPR020479

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN001216052

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR017970

(InterPro Project Members, 2004-)

enables DNA-binding transcription factor activity

inferred from electronic annotation with InterPro:IPR042634

(InterPro Project Members, 2004-)

enables RNA polymerase II cis-regulatory region sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN001216052

(GO Reference Genome Project, 2011-)

Biological Process (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in positive regulation of transcription by RNA polymerase II

inferred from electronic annotation with InterPro:IPR042634

(InterPro Project Members, 2004-)

involved_in regulation of DNA-templated transcription

inferred from electronic annotation with InterPro:IPR017970

(InterPro Project Members, 2004-)

involved_in regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN001216052

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN001216052

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

HOX-LIKE HOMEOBOX TRANSCRIPTION FACTORS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

buttonless (btn) encodes a protein that contributes to cuticle pigmentation. [Date last reviewed: 2019-09-12]

Gene Group (FlyBase)

HOX-LIKE HOMEOBOX TRANSCRIPTION FACTORS -: HOX-like (HOXL) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. They encompass transcription factors encoded by the Hox genes of the Antennapedia and the Bithorax gene complexes and genes closely related in sequence. HOXL transcription factors are major regulators of animal development. (Adapted from FBrf0232555).

Summary (Interactive Fly)

transcription factor - homeodomain - regulates the identity of the dorsal median cells that are arranged as a single pair within each segment along the dorsal midline just above the central nervous system C

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\btn for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VCY0)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.47

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

btn-RA

FBtr0084278

NM_170026

477

158

Additional Transcript Data and Comments

Reported size (kB)

0.6~0.7 (northern blot)

(Chiang et al., 1994)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

btn-PA

FBpp0083671

18.7

158

8.44

Q9VCY0

NP_732768

AAF56025

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

158 (aa)

(Chiang et al., 1994)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\btn using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

btn_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

NA

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4 -- 6

central brain anlage in statu nascendi

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

antennal anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

dorsal head epidermis anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

visual anlage in statu nascendi

Comment: reported as procephalic ectoderm anlage in statu nascendi

(Fisher et al., 2012)

embryonic stage 7 -- 8

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

larval foregut atrium | precursor

Comment: reported as atrium primordium

(Fisher et al., 2012)

midline primordium

(Fisher et al., 2012)

ventral epidermis primordium

(Fisher et al., 2012)

ventral head epidermis primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- first instar larval stage

larval thoracic perisympathetic organ

(Chiang et al., 1994)

embryonic stage 13 -- 16

larval foregut atrium

(Fisher et al., 2012)

embryonic ventral epidermis

(Fisher et al., 2012)

ventral midline of embryo

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Chiang et al., 1994)

embryonic stage 11 -- 17

larval thoracic perisympathetic organ

(Chiang et al., 1994)

Additional Descriptive Data

Northern blot analysis indicates that the rare btn transcript is expressed throughout the embryonic period from about 4hrs on and more weakly in larval, pupal and adult periods. Expression is first detected by in situ hybridization in a group of two to four midline cells in the three thoracic and first seven abdominal segments at stage 11. They are located at the position of future segment boundaries and are found along the dorsal midline of the mesodermal bridge. During germ band retraction the btn-expressing cells extend lateral processes. By stage 14, the processes extend well beyond the ventral cord to the sites of muscle attachment. The number of btn-expressing cells at this stage is reduced from four to two. These mature cells are the dorsal neurohemal organs also known as dorsal median cells.

(Chiang et al., 1994)

Marker for

larval thoracic perisympathetic organ

(Chiang et al., 1994)

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\btn in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\btn

View exonic expression by tissue for Dmel\btn

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014949

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42664/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014949

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014949

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014949

Flygut - An atlas of the Drosophila adult midgut

FBgn0014949

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of btn

Transgenic constructs containing regulatory region of btn

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(3R)Exel6273

(Ryder, 2004.4.26)

Inferred from location ( 5 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die during embryonic stage | recessive

btn^r3

partially lethal - majority die | recessive

btn^S126113b

viable

btn^CB03501

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

btn^GD4160

viable, with Scer\GAL4^Mef2.PR

btn^GD4160

viable, with Scer\GAL4^pnr-MD237

btn^GD4160

viable, with Scer\GAL4^tin.CΔ4

Other Phenotypes

Allele

some die during embryonic stage | recessive

btn^r3

visible | male limited, with Scer\GAL4^Bx-MS1096

btn^{UAS.ORF.GW.Tag:HA}

Phenotype manifest in

Allele

abdominal tergite 5 | female, with Scer\GAL4^Tub.PU

btn^KK102048

abdominal tergite 6 | female, with Scer\GAL4^pnr-MD237

btn^KK102048

abdominal tergite 6 | female, with Scer\GAL4^Tub.PU

btn^KK102048

larval thoracic perisympathetic organ

btn^r3

larval transverse nerve

btn^r3

macrochaeta

btn^BG01960

transverse nerve & mesothoracic segment

btn^r3

transverse nerve & metathoracic segment

btn^r3

transverse nerve & prothoracic segment

btn^r3

wing | male limited, with Scer\GAL4^Bx-MS1096

btn^{UAS.ORF.GW.Tag:HA}

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (153)

Hsap\MEOX1

8 of 14

Yes

Hsap\MEOX2

7 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

Hsap\MNX1

3 of 14

Hsap\PDX1

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\DUX4L7

1 of 14

Yes

Hsap\DUX4L9

1 of 14

Yes

Hsap\DUXA

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Hsap\LOC112268164

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (120)

Rnor\Meox1

8 of 14

Yes

Rnor\Meox2

7 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (122)

Mmus\Meox1

8 of 14

Yes

Mmus\Meox2

7 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Mmus\Rhox11

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (145)

Xtro\LOC101731251

5 of 13

Yes

Xtro\meox2

5 of 13

Yes

Xtro\hoxa4

4 of 13

Xtro\hoxa7

4 of 13

Xtro\hoxc5

4 of 13

Yes

Xtro\kif2a

4 of 13

Xtro\cbx5

3 of 13

Xtro\hoxa9

3 of 13

Yes

Xtro\hoxb5

3 of 13

Xtro\hoxb6

3 of 13

Yes

Xtro\hoxb8

3 of 13

Xtro\hoxc3

3 of 13

Yes

Xtro\hoxc6

3 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Xtro\hoxb2

2 of 13

Xtro\hoxb3

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\soho1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (159)

Drer\meox1

8 of 14

Yes

Drer\meox2a

7 of 14

Yes

Drer\meox2b

7 of 14

Yes

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

Yes

4 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (52)

Cele\mab-5

5 of 14

Yes

Cele\lin-39

4 of 14

Cele\ceh-12

3 of 14

Cele\ceh-13

3 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

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1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (58)

Agam\AgaP_AGAP002372

4 of 12

Yes

Agam\AgaP_AGAP004646

4 of 12

Yes

Agam\AgaP_AGAP004647

4 of 12

Yes

Agam\AgaP_AGAP004648

4 of 12

Yes

Agam\AgaP_AGAP004659

4 of 12

Yes

Agam\AgaP_AGAP005041

4 of 12

Yes

Agam\AgaP_AGAP006923

4 of 12

Yes

Agam\AgaP_AGAP013157

4 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (31)

Atha\ATHB13

4 of 13

Yes

Atha\AtHB23

4 of 13

Yes

Atha\ATHB54

4 of 13

Yes

Atha\HB-1

4 of 13

Yes

Atha\HB-3

4 of 13

Yes

Atha\HB5

4 of 13

Yes

Atha\HB6

4 of 13

Yes

Atha\HB-7

4 of 13

Yes

Atha\HB16

4 of 13

Yes

Atha\HB20

4 of 13

Yes

Atha\HB22

4 of 13

Yes

Atha\HB51

4 of 13

Yes

Atha\HAT1

3 of 13

Yes

Atha\HAT2

3 of 13

Yes

Atha\HAT3

3 of 13

Yes

Atha\HAT9

3 of 13

Yes

Atha\HAT14

3 of 13

Yes

Atha\HAT22

3 of 13

Yes

Atha\HB-2

3 of 13

Yes

Atha\HB4

3 of 13

Yes

Atha\HB-12

3 of 13

Atha\HB17

3 of 13

Yes

Atha\HB18

3 of 13

Yes

Atha\HB21

3 of 13

Yes

Atha\HB40

3 of 13

Yes

Atha\HB52

3 of 13

Yes

Atha\HB53

3 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (2)

Scer\YHP1

2 of 13

Yes

Scer\YOX1

2 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\phx1

1 of 12

Yes

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:btn. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (80)

zen2

6 of 13

Antp

5 of 13

Dfd

5 of 13

exex

5 of 13

ftz

5 of 13

lab

5 of 13

Scr

5 of 13

zen

5 of 13

abd-A

4 of 13

bcd

4 of 13

cad

4 of 13

Dbx

4 of 13

ems

4 of 13

HGTX

4 of 13

lms

4 of 13

Ubx

4 of 13

unpg

4 of 13

bap

3 of 13

B-H2

3 of 13

bsh

3 of 13

CG18599

3 of 13

CG34031

3 of 13

eve

3 of 13

HHEX

3 of 13

Hmx

3 of 13

ind

3 of 13

inv

3 of 13

NK7.1

3 of 13

tin

3 of 13

Abd-B

2 of 13

B-H1

2 of 13

C15

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Dll

2 of 13

Drgx

2 of 13

gsb

2 of 13

gsb-n

2 of 13

H2.0

2 of 13

hbn

2 of 13

lbe

2 of 13

lbl

2 of 13

Lim1

2 of 13

OdsH

2 of 13

otp

2 of 13

PHDP

2 of 13

prd

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

vnd

2 of 13

Vsx1

2 of 13

Awh

1 of 13

CG4328

1 of 13

CG32532

1 of 13

eyg

1 of 13

Gsc

1 of 13

Lim3

1 of 13

Lmx1a

1 of 13

Pph13

1 of 13

toe

1 of 13

toy

1 of 13

tup

1 of 13

Vsx2

1 of 13

zfh1

1 of 13

zfh2

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 5 )

Human Ortholog

Disease

Evidence

References

MEOX1; mesenchyme homeobox 1

model of Klippel-Feil syndrome 2

IEA

(FlyBase, 2019-)

PDX1; pancreatic and duodenal homeobox 1

model of pancreatic agenesis 1

IEA

(FlyBase, 2019-)

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

model of maturity-onset diabetes of the young type 4

IEA

(FlyBase, 2019-)

PAX4; paired box 4

model of type 2 diabetes mellitus

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MEOX1; mesenchyme homeobox 1

8 of 14

600147

KLIPPEL-FEIL SYNDROME 2, AUTOSOMAL RECESSIVE; KFS2

Klippel-Feil syndrome 2

MEOX2; mesenchyme homeobox 2

7 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

4 of 14

GBX1; gastrulation brain homeobox 1

3 of 14

603354

GBX2; gastrulation brain homeobox 2

3 of 14

3 of 14

3 of 14

MICROTIA, HEARING IMPAIRMENT, AND CLEFT PALATE

3 of 14

3 of 14

FACIAL PARESIS, HEREDITARY CONGENITAL, 3; HCFP3

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

MNX1; motor neuron and pancreas homeobox 1

3 of 14

142994

CURRARINO SYNDROME

Currarino syndrome

PDX1; pancreatic and duodenal homeobox 1

3 of 14

600733

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

btn - CG10654

two hybrid array

(Shokri et al., 2019)

btn - CG14667

two hybrid array

(Shokri et al., 2019)

btn - CG8924

two hybrid array

(Shokri et al., 2019)

btn - Ets65A

two hybrid array

(Shokri et al., 2019)

btn - Hr78

two hybrid array

(Shokri et al., 2019)

btn - Sag1

two hybrid array

(Shokri et al., 2019)

btn - da

two hybrid array

(Shokri et al., 2019)

btn - sage

two hybrid array

(Shokri et al., 2019)

btn - soul

two hybrid array

(Shokri et al., 2019)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nsf2

suppressible

btn

(Laviolette et al., 2005)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0014949

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-75

Cytogenetic map

94B5-94B5

Sequence location

3R:22,586,149..22,586,625 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94B5-94B5

Limits computationally determined from genome sequence between P{lacW}Dph5^L4910 and P{lacW}Gclm^L0580

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

94B5-94B10

(determined by in situ hybridisation)

(Deak, 1999.12.22, Deak et al., 1997)

94B-94B

(Zhang, 1999.2.6)

94B-94B

(determined by in situ hybridisation)

(Chiang et al., 1994)

Experimentally Determined Recombination Data

Location

3-75

(FlyBase, 2016)

3-77.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

45914

w¹¹¹⁸; P{GMR86E01-GAL4}attP2

54287

w¹¹¹⁸; P{GMR86E01-lexA}attP40/CyO

94649

w¹¹¹⁸; PBac{GMR86E01-lexA}VK00027

42530

y¹ v¹; P{TRiP.HMJ02097}attP40

56154

y¹ w^*; PBac{btn-GFP.FPTB}VK00037

15332

y¹ w^67c23; P{EPgy2}btn^EY01209

79834

y¹ sc^* v¹ sev²¹; P{TOE.GS02414}attP40

FlyORF

F000449

M{UAS-btn.ORF.3xHA.GW}ZH-86Fb

NIG-Fly

HMJ02097

y¹ v¹; P{TRiP.HMJ02097}attP40/CyO

VDRC

v30465

w¹¹¹⁸; P{GD4160}v30465

v50658

w¹¹¹⁸; P{GD17511}v50658

v341872

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01060}attP40/CyO-GFP

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (1)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

RT01054

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0014949

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0014949

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Proper btn gene expression and dorsal median cell differentiation requires signals from underlying CNS midline cells.

(Zhou et al., 1997)

btn gene function is critical for the specification of dorsal median cell fate and for transverse nerve pathfinding.

(Chiang et al., 1994)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

1261/13

(Deak et al., 1997)

BTN

(Kim et al., 2010)

CG5264

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Laviolette et al., 2005, Norga et al., 2003)

btn

(Sun et al., 2023, Shokri et al., 2019, Transgenic RNAi Project members, 2017-, Bürglin and Affolter, 2016, Bivik et al., 2015, Dembeck et al., 2015, Schertel et al., 2015, Victorsen and White, 2014.5.13, Jin et al., 2013, Buszczak et al., 2007, Hoskins et al., 2005, Laviolette et al., 2005)

btn/MEOX

(Coulier et al., 2000)

Name Synonyms

Buttonless

(Kim et al., 2010)

buttonless

(Zhang, 1999.2.6, Chiang et al., 1994)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

42664

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VCY0

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VCY0

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0014949

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

42664/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0014949

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0014949

FlyMine - An integrated database for Drosophila genomics

FBgn0014949

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG5264

MARRVEL_MODEL - MARRVEL (model organism gene)

42664

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0014949

DroID - A comprehensive database of gene and protein interactions.

FBgn0014949

DRSC - Results frm RNAi screens

FBgn0014949

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

btn_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0014949

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0014949

Flygut - An atlas of the Drosophila adult midgut

FBgn0014949

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

References (60)

The following external sites may use different assemblies or annotations than FlyBase.

NA

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