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Gene Dmel\Actn3

General Information
Symbol	Dmel\Actn3	Species	D. melanogaster
Name	α actinin 3	Annotation symbol	CG8953
Feature type	protein_coding_gene	FlyBase ID	FBgn0015008
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	acta-3
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	89E5-89E5	Sequence location	3R:12,801,607..12,803,517 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene α actinin 3 is referred to in FlyBase by the symbol Dmel\Actn3 (CG8953, FBgn0015008). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: calcium ion binding; actin filament binding; actin binding. Based on sequence similarity, it is predicted to be involved in the biological process: actin filament bundle assembly. 4 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Calponin homology domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1636013_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Actn3. Gene sequence location is 3R:12801607..12803517.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10
FB2012_01	Sequence features BKN23709 BKN45650 HFA14106
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 89E5-89E5   Limits computationally determined from genome sequence between P{lacW}CSN5L4032 and P{EP}MESK4EP1015
Experimentally Determined Cytological Location
Cytogenetic map Notes References 89E3-89E3     (O'Brien, 2001)
Experimentally Determined Recombination Data
Location	3-
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Actn3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Actn3-RA FBtr0083380  NM_080062   1856   553
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Actn3-PA   FBpp0082822   63.3   553   9.99     NP_524801   AAF55366
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Calponin homology domain (IPR001715)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF55366   BT023197   EL870784   EL870785   EL870937   U31961 AAA84401
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24109 Q9VEQ5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0015008    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0015008 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed during late larval stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0015008 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2  (108) larva L3 puffstage 3-6  (266) larva L3 puffstage 7-9  (412) white prepupae new  (341) white prepupae 12hr  (591) white prepupae 24hr  (848) pupae 2d postWPP  (1178) pupae 3d postWPP  (1020) pupae 4d postWPP  (421) adult male 01day  (1209) adult male 05day  (1155) adult male 30day  (887) adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0015008 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2  108 larva L3 puffstage 3-6  (266) larva L3 puffstage 7-9  (412) white prepupae new  (341) white prepupae 12hr  (591) white prepupae 24hr  (848) pupae 2d postWPP  (1178) pupae 3d postWPP  (1020) pupae 4d postWPP  (421) adult male 01day  (1209) adult male 05day  (1155) adult male 30day  (887) adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    1 embryo 04-06hr    1 embryo 06-08hr    3 embryo 08-10hr    0 embryo 10-12hr    1 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    1 embryo 18-20hr    0 embryo 20-22hr    0 embryo 22-24hr    0 larva L1    0 larva L2    0 larva L3 12hr old    0 larva L3 puffstage 1-2    108 larva L3 puffstage 3-6    266 larva L3 puffstage 7-9    412 white prepupae new    341 white prepupae 12hr    591 white prepupae 24hr    848 pupae 2d postWPP    1178 pupae 3d postWPP    1020 pupae 4d postWPP    421 adult male 01day    1209 adult male 05day    1155 adult male 30day    887 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0015008    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0015008 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0015008 expression level Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis    559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis  (559.9) Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis    559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis    559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0015008 expression level Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis    559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis  559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis    559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.175 Larval Midgut    7 Larval Hindgut    2.6 Larval Malpighian Tubules    4.8 Larval Fat Body no informative data Larval Salivary Gland    6.4 Larval Trachea    4.35 Larval Carcass    9.975 Adult Head    2.6 Adult Eye    3.1 Adult Brain    3.6 Adult Thoracic-Abdominal Ganglion    4.5 Adult Crop    2.3 Adult Midgut    2 Adult Hindgut    2.1 Adult Malpighian Tubules    5.2 Adult Fat Body    6.5 Adult Salivary Gland no informative data Adult Heart    2 Adult VirginFemale Spermatheca    7 Adult InseminatedFemale Spermatheca    7.4 Adult Ovary    2.6 Adult Testis    559.9 Adult Male Accessory Gland no informative data Adult Carcass    7.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland no informative data Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    2.6   NA  Eye    3.1   NA  Brain    3.6   2.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    4.5   NA  Crop    2.3   7  Midgut    2   2.6  Hindgut    2.1   4.8  Malpighian Tubules    5.2   no informative data  Fat Body    6.5   6.4  Salivary Gland    no informative data   NA  Heart    2   4.35  Trachea    NA   NA  VirginFemale Spermatheca    7   NA  InseminatedFemale Spermatheca    7.4   NA  Ovary    2.6   NA  Testis    559.9   NA  Male Accessory Gland    no informative data   9.975  Carcass    7.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015008 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8953-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 Actn3GD4964 Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Actn3 Class Mutagen Stocks Known lesion Actn3f00971 piggyBac transposase 2 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Actn3 Class Mutagen Stocks Known lesion Actn3GD4964 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Actn3JF02279 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Actn3KK103517 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4964} NA P{KK103517} NA P{TRiP.JF02279} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{WH}Actn3f00971 NA
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
actin binding inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) non-traceable author statement (O'Brien, 2001) actin filament binding inferred from sequence or structural similarity with EMBL:AF093775 (FlyBase, 1992-) calcium ion binding inferred from sequence or structural similarity with EMBL:AF093775 (FlyBase, 1992-)
Biological Process
CV term Evidence References
actin filament bundle assembly inferred from sequence or structural similarity with EMBL:AF093775 (FlyBase, 1992-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Actn3 allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0015008
Orthologs
Genome-wide drosophilid orthologs	Dana\GF17920 Dere\GG16805 Dmoj\GI19826 Dsec\GM15399 Dsim\GD20264 Dvir\GJ18586 Dyak\GE26122
Curated drosophilid orthologs	Dpse\GA21431
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • ENSECAP00000008079 Equus caballus (horse) ENSP00000382108 Homo sapiens (human) ENSPPYP00000003759 Pongo pygmaeus (orangutan) ENSPTRP00000056686 Pan troglodytes (chimpanzee) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0243161 FBgn0205744 FBgn0191741 FBgn0170318 FBgn0261490 FBgn0142563 FBgn0109035 FBgn0094938
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	26737 y1 v1; P{TRiP.JF02279}attP2 18397 w1118; PBac{WH}Actn3f00971/TM6B, Tb1
Harvard	- PBac{WH}Actn3f00971
VDRC	v106162 P{KK103517}VIE-260B v15171 w1118; P{GD4964}v15171 v15172 w1118; P{GD4964}v15172/CyO
Genomic Clones ( 1 )
	BACR03M20 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 2 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	IP03156
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	IP02856
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015008 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44964
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Actn3 dabp (O'Brien, 2001.3.12)
Source for database merge of	Source for merge of: Actn3 CG8953 (Bayraktaroglu, 2000.4.13)
Additional comments
Other Comments
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080062 RefSeq (Proteins) • NP_524801 DDBJ / EMBL / GenBank • AAA84401 AAF55366 U31961 Entrez Gene - A searchable database of RefSeq genes. • 44964 UniProtKB/TrEMBL • Q9VEQ5 Q24109
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Calponin homology domain (IPR001715)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0015008 DRSC - Results from RNAi screens. • FBgn0015008 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015008 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8953-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015008 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44964 InParanoid A subset of ortholog calls from InParanoid. • ENSECAP00000008079 Equus caballus (horse) ENSP00000382108 Homo sapiens (human) ENSPPYP00000003759 Pongo pygmaeus (orangutan) ENSPTRP00000056686 Pan troglodytes (chimpanzee) modMine - Data generated by the modENCODE project. • FBgn0015008 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0243161 FBgn0205744 FBgn0191741 FBgn0170318 FBgn0261490 FBgn0142563 FBgn0109035 FBgn0094938
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	acta-3 (Moshkin et al., 2001, Zhimulev et al., 2003) Actn3   CG8953 (O'Brien, 2001.3.12, Goldstein and Gunawardena, 2000, Perkins et al., 2009.3.31, Keleman et al., 2009.8.5) dabp (O'Brien, 2001) ORF1 (Martin et al., 1995)
Name Synonym	open reading frame 1 (Martin et al., 1995) α actinin 3   α-actinin3 (Alves-Silva et al., 2008) α-actinin-like (Martin et al., 1995)
Secondary FlyBase IDs
	FBgn0038459
References ( 17 )
Research paper	Alves-Silva et al., 2008, Mol. Biol. Cell 19(10): 4287--4297 Prominent actin fiber arrays in Drosophila tendon cells represent architectural elements different from stress fibers. [FBrf0207329] Moshkin et al., 2001, Proc. Natl. Acad. Sci. USA 98(2): 570--574 The Bithorax complex of Drosophila melanogaster. Underreplication and morphology in polytene chromosomes. [FBrf0134831] Singh et al., 1997, Nucleic Acids Res. 25(7): 1419--1425 Mathematical model to predict regions of chromatin attachment to the nuclear matrix. [FBrf0093731] Martin et al., 1995, Proc. Natl. Acad. Sci. USA 92(18): 8398--8402 Complete sequence of the bithorax complex of Drosophila. [FBrf0083285]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518] Goldstein and Gunawardena, 2000, J. Cell Biol. 150(2): Table S2. Identified cytoskeletal protein coding genes in Drosophila. [FBrf0132100]
Review	Zhimulev et al., 2003, Genetica 117(2-3): 259--270 Intercalary heterochromatin in Drosophila melanogaster polytene chromosomes and the problem of genetic silencing. [FBrf0158966]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Perkins et al., 2009.3.31, TRiP stock collection set 2. TRiP stock collection set 2. [FBrf0207631] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] O'Brien, 2001.3.12, Helping FlyBase: ADRC-10089. Helping FlyBase: ADRC-10089. [FBrf0135340] Bayraktaroglu, 2000.4.13, Annotation identities: Category 9. Annotation identities: Category 9. [FBrf0126913]
Abstract	O'Brien, 2001, A. Dros. Res. Conf. 42: 213C Cloning and characterization of a novel actin binding protein in Drosophila melanogaster. [FBrf0133472]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Curated genome annotation	Milshina, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126686]