FlyBase Gene Report: Dmel\CkIα

General Information

Symbol

Dmel\CkIα

Species

D. melanogaster

Name

Casein kinase Iα

Annotation Symbol

CG2028

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015024

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Snapshot

Casein kinase Iα (CkIα) encodes a member of the CK1 family of Ser/Thr kinases with a preference for acidic substrates. It is involved in multiple signaling pathways, including Wingless and Hedgehog, that control cell growth and patterning. [Date last reviewed: 2019-03-07]

Other Summaries

Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

CK1, CK1α, CKI, CK I, CK1alpha

Key Links

Genomic Location

Cytogenetic map

11B7-11B8

Sequence location

X:12,653,626..12,657,834 [+]

Recombination map

1-39

RefSeq locus

NC_004354 REGION:12653626..12657834

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (17 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with UniProtKB:O16844

(assigned by UniProt )

(Zhang et al., 2005)

enables protein serine/threonine kinase activity

inferred from direct assay

(Fulford et al., 2019, Swarup and Verheyen, 2011, Zhang et al., 2006, Matsubayashi et al., 2004, Yanagawa et al., 2002, Santos et al., 1996)

inferred from mutant phenotype

(Zhang et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000225966

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (11 terms)

Terms Based on Experimental Evidence (10 terms)

CV Term

Evidence

References

involved_in cell morphogenesis

inferred from mutant phenotype

(D'Ambrosio and Vale, 2010)

involved_in DNA repair

inferred from direct assay

(Santos et al., 1996)

involved_in glucose homeostasis

inferred from mutant phenotype

(Ugrankar et al., 2015)

involved_in negative regulation of canonical Wnt signaling pathway

inferred from mutant phenotype

(Legent et al., 2012, Zhang et al., 2006, Matsubayashi et al., 2004, Lum et al., 2003, Yanagawa et al., 2002)

involved_in negative regulation of hippo signaling

inferred from mutant phenotype

(Fulford et al., 2019)

involved_in negative regulation of smoothened signaling pathway

inferred from mutant phenotype

(Apionishev et al., 2005, Jia et al., 2005, Lum et al., 2003)

involved_in positive regulation of proteasomal protein catabolic process

inferred from mutant phenotype

(Legent et al., 2012)

involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Galletti et al., 2009, Yanagawa et al., 2002)

inferred from mutant phenotype

(assigned by ParkinsonsUK-UCL )

(Liu et al., 2002)

involved_in positive regulation of smoothened signaling pathway

inferred from mutant phenotype

(Apionishev et al., 2005, Jia et al., 2004, Zhang et al., 2004)

inferred from genetic interaction with FLYBASE:dco; FB:FBgn0002413

(Shi et al., 2014)

inferred from genetic interaction with FLYBASE:smo; FB:FBgn0003444

(Zhou and Kalderon, 2011)

involved_in protein phosphorylation

inferred from direct assay

(Zhang et al., 2006)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in negative regulation of canonical Wnt signaling pathway

inferred by curator from GO:0032436

(assigned by ParkinsonsUK-UCL )

(Liu et al., 2002)

inferred from biological aspect of ancestor with PANTHER:PTN000936058

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in peptidyl-serine phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000225966

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Zhang et al., 2006, Santos et al., 1996)

inferred from direct assay

(assigned by UniProt )

(Zhang et al., 2005)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Zhang et al., 2005)

inferred from direct assay

(Zhang et al., 2006, Santos et al., 1996)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

part_of beta-catenin destruction complex

inferred from sequence or structural similarity with UniProtKB:P48729

(assigned by ParkinsonsUK-UCL )

(Neurological Gene Annotation Project members, 2014-)

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000225966

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000225966

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CK1 KINASES

BETA CATENIN DESTRUCTION COMPLEX

Pathway (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway

Negative Regulators of Hedgehog Signaling Pathway

Positive Regulators of Hedgehog Signaling Pathway

Negative Regulators of Hippo Signaling Pathway

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily. (P54367)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CK1 KINASES -: The CK1 kinases (Casein Kinase 1 family) share a highly conserved kinase domain. CK1 kinases are serine/threonine kinases with a preference for acidic substrates. The highly variable C-terminal domains are involved in regulating subcellular localization and activity. (Adapted from FBrf0132098, PMID:24825444 and PMID:15722192).
BETA CATENIN DESTRUCTION COMPLEX -: The β-catenin destruction complex is a cytoplasmic protein complex that phosphorylates β-catenin, targeting it for ubiquitin-mediated proteolysis. In the absence of Wnt signaling, the β-catenin destruction complex constitutively suppresses the canonical Wnt signaling pathway by preventing the accumulation of cytoplasmic β-catenin (arm). (Adapted from FBrf0217546).

Pathway (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Protein Function (UniProtKB)

Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Can phosphorylate a large number of proteins. Negative regulator of wg signaling (PubMed:22095083). Phosphorylates arm directly or indirectly and stimulates its degradation which prevents inappropriate wg signaling (PubMed:11955436, PubMed:11927557, PubMed:22095083). Phosphorylates smo which promotes its accumulation at the cell surface and its signaling activity in response to hh (PubMed:15616566). Together with dco, regulates proteolytic processing of ci by phosphorylating it which promotes its binding to slmb, the F-box recognition component of the SCF(slmb) E3 ubiquitin-protein ligase required for ci processing (PubMed:16326393). Inhibits condensin II interphase activity by promoting degradation of the Cap-H2 regulatory subunit and limiting the levels of chromatin-bound Cap-H2 which regulates interphase chromosome organization (PubMed:25723539).

(UniProt, P54367)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CkIα for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CkIα-RA

FBtr0073682

NM_167331

2133

337

CkIα-RB

FBtr0073680

NM_078585

2791

337

CkIα-RD

FBtr0300380

NM_001169266

2232

337

CkIα-RE

FBtr0340408

NM_001298234

2406

337

CkIα-RF

FBtr0345162

NM_001298235

1997

337

CkIα-RG

FBtr0345163

NM_167332

1997

337

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

CkIα-PA

FBpp0073515

39.5

337

9.80

NP_727631

AAN09313

CkIα-PB

FBpp0073513

39.5

337

9.80

NP_511140

AAF48193

CkIα-PD

FBpp0289609

39.5

337

9.80

NP_001162737

ACZ95272

CkIα-PE

FBpp0309354

39.5

337

9.80

NP_001285163

AHN59633

CkIα-PF

FBpp0311372

39.5

337

9.80

NP_001285164

AHN59634

CkIα-PG

FBpp0311373

39.5

337

9.80

NP_727632

AAF48192

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

337 aa isoforms: CkIα-PA, CkIα-PB, CkIα-PD, CkIα-PE, CkIα-PF, CkIα-PG

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with cos.

(UniProt, P54367)

Post Translational Modification

Phosphorylated. The dephosphorylated kinase is active in the cytoplasm while the active kinase in the nucleus is phosphorylated.

(UniProt, P54367)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CkIα using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in cytoplasm

inferred from direct assay

(Zhang et al., 2006, Santos et al., 1996)

inferred from direct assay

(assigned by UniProt )

(Zhang et al., 2005)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Zhang et al., 2005)

inferred from direct assay

(Zhang et al., 2006, Santos et al., 1996)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\CkIα in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CkIα

View exonic expression by tissue for Dmel\CkIα

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015024

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015024

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2028-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2028

Flygut - An atlas of the Drosophila adult midgut

FBgn0015024

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CkIα

Transgenic constructs containing regulatory region of CkIα

Deletions and Duplications ( 1 )

Duplicated in

Dp(1;3)DC257

(Popodi et al., 2010-, Venken et al., 2010)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

CkIα^GD4256

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CkIα^GD4256

lethal | embryonic stage

CkIα^8B12

lethal | larval stage

CkIα^8B12

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

CkIα^GD4256

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CkIα^GD4256

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

CkIα^GD4256

partially lethal - majority die | recessive

CkIα^G0492

viable, with Scer\GAL4^tin.CΔ4

CkIα^GD4256

Sterility

Allele

female sterile

CkIα^G0492

female sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

CkIα^HMS02276

Other Phenotypes

Allele

behavior defective | recessive

CkIα^BG01498

melanotic mass phenotype, with Scer\GAL4^{SPARC-MI00329-GAL4}

CkIα^GL00021

neuroanatomy defective | larval stage

CkIα^GD4256

some die during pupal stage, with Scer\GAL4^pnr-MD237

CkIα^GD4256

visible, with Scer\GAL4^GMR.PF

CkIα^EP1555

visible | somatic clone

CkIα^8B12

Phenotype manifest in

Allele

eye, with Scer\GAL4^GMR.PF

CkIα^EP1555

eye | somatic clone

CkIα^8B12

eye disc | somatic clone | third instar larval stage

CkIα^8B12

fat body, with Scer\GAL4^{SPARC-MI00329-GAL4}

CkIα^GL00021

ganglion mother cell | larval stage

CkIα^GD4256

larval neuroblast | larval stage

CkIα^GD4256

melanotic mass, with Scer\GAL4^{SPARC-MI00329-GAL4}

CkIα^GL00021

neuromuscular junction, with Scer\GAL4^elav-C155

synapse, with Scer\GAL4^elav-C155

wing | somatic clone

CkIα^8B12

wing margin bristle | ectopic, with Scer\GAL4^Bx-MS1096

CkIα^dsRNA.UAS

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CSNK1A1

15 of 15

Yes

Hsap\CSNK1A1L

11 of 15

Yes

5 of 15

3 of 15

3 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Csnk1a1

15 of 15

Yes

5 of 15

4 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (10)

Rnor\Csnk1a1

9 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (10)

Xtro\csnk1a1

10 of 12

Yes

4 of 12

4 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (13)

Drer\csnk1a1

13 of 15

Yes

5 of 15

5 of 15

5 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (90)

Cele\kin-19

12 of 15

Yes

Cele\kin-20

3 of 15

Cele\csnk-1

2 of 15

Cele\prde-1

2 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (14)

Atha\ADK1

4 of 9

Yes

Atha\CKI1

4 of 9

Yes

Atha\ckl7

4 of 9

Yes

Atha\ckl8

4 of 9

Yes

Atha\ckl10

4 of 9

Yes

Atha\CKL13

4 of 9

Yes

3 of 9

3 of 9

3 of 9

3 of 9

3 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\HRR25

7 of 15

Yes

Scer\YCK1

4 of 15

Scer\YCK2

4 of 15

Scer\YCK3

3 of 15

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\hhp1

5 of 12

Yes

4 of 12

2 of 12

2 of 12

2 of 12

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190AXE )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15193

Drosophila persimilis

Dper\GL13140

Drosophila willistoni

Dwil\GK25517

Drosophila virilis

Dvir\GJ15916

Drosophila mojavensis

Dmoj\GI16225

Drosophila grimshawi

Dgri\GH12595

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506HF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17026159

Glossina morsitans

Tsetse fly

Gmor\GMOY011765

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_00102

Mayetiola destructor

Hessian fly

Mdes\Mdes007835

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007662

Anopheles darlingi

American malaria mosquito

Adar\ADAC003068

Anopheles gambiae

Malaria mosquito

Agam\AGAP000627

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017982

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ017578

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W07IJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007304

Bombyx mori

Silkmoth

Bmor\BGIBMGA014220

Danaus plexippus

Monarch butterfly

Dple\DPOGS200797-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS200370-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL016957

Heliconius melpomene

Postman butterfly

Hmel\HMEL004961

Apis florea

Little honeybee

Aflo\2331144004

Apis florea

Little honeybee

Aflo\2379349796

Apis florea

Little honeybee

Aflo\2147680531

Apis mellifera

Western honey bee

Amel\Csnk1a1

Apis mellifera

Western honey bee

Amel\GB55165

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16186

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP14828

Bombus terrestris

Buff-tailed bumblebee

Bter\15209593

Bombus terrestris

Buff-tailed bumblebee

Bter\15205615

Linepithema humile

Argentine ant

Lhum\LH18256

Linepithema humile

Argentine ant

Lhum\LH16762

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002c48

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:002cde

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG018299

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001647

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG018300

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05765

Tribolium castaneum

Red flour beetle

Tcas\TC034052

Tribolium castaneum

Red flour beetle

Tcas\TC012576

Pediculus humanus

Human body louse

Phum\PHUM327780

Pediculus humanus

Human body louse

Phum\PHUM456320

Rhodnius prolixus

Kissing bug

Rpro\dbt

Cimex lectularius

Bed bug

Clec\CLEC000485

Cimex lectularius

Bed bug

Clec\CLEC000706

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI008162

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007630

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03873

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04955

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X07DP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013208

Strigamia maritima

European centipede

Smar\SMAR015418

Ixodes scapularis

Black-legged tick

Isca\ISCW002293

Ixodes scapularis

Black-legged tick

Isca\ISCW007936

Ixodes scapularis

Black-legged tick

Isca\ISCW001795

Stegodyphus mimosarum

African social velvet spider

Smim\KFM71835.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM73238.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g06080

Tetranychus urticae

Two-spotted spider mite

Turt\tetur10g00680

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_232195

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_301140

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AFG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ck1a2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ck1d

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ck1a1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000006500

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000002874

Ciona intestinalis

Vase tunicate

Cint\LOC100177660

Gallus gallus

Domestic chicken

Ggal\CSNK1A1

Gallus gallus

Domestic chicken

Ggal\CSNK1D

Gallus gallus

Domestic chicken

Ggal\CSNK1E

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (11)

Gene Symbol

Score

Source

Alignment

dco

7 of 10

6 of 10

6 of 10

5 of 10

4 of 10

4 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CSNK1A1; casein kinase 1 alpha 1

15 of 15

600505

CSNK1A1L; casein kinase 1 alpha 1 like

11 of 15

CSNK1D; casein kinase 1 delta

5 of 15

600864

ADVANCED SLEEP PHASE SYNDROME, FAMILIAL, 2; FASPS2

advanced sleep phase syndrome 2

CSNK1E; casein kinase 1 epsilon

3 of 15

600863

TPTEP2-CSNK1E; TPTEP2-CSNK1E readthrough

3 of 15

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CkIα - CG15710

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

CkIα - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

CkIα - arm

enzymatic study, western blot, protein kinase assay, autoradiography

(Matsubayashi et al., 2004, Yanagawa et al., 2002)

CkIα - ci

anti tag coimmunoprecipitation, anti tag western blot

(Shi et al., 2011)

CkIα - cos

anti tag coimmunoprecipitation, anti tag western blot

(Shi et al., 2011)

CkIα - dco

experimental knowledge based

(Guruharsha et al., 2011)

CkIα - per

anti tag coimmunoprecipitation, anti tag western blot

(Lam et al., 2018)

CkIα - slmb

anti tag coimmunoprecipitation, anti tag western blot

(Fulford et al., 2019)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

brat

enhanceable

CkIα

(Komori et al., 2014)

dco

enhanceable

CkIα

(Strutt et al., 2006)

suppressible

CkIα

(Müller et al., 2005)

External Data

Subunit Structure (UniProtKB)

Interacts with cos.

(UniProt, P54367 )

Linkouts

BioGRID - A database of protein and genetic interactions.

58615

DroID - A comprehensive database of gene and protein interactions.

FBgn0015024

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32221

MIST (genetic) - An integrated Molecular Interaction Database

CkIalpha

MIST (protein-protein) - An integrated Molecular Interaction Database

CkIalpha

Pathways

Signaling Pathways (FlyBase)

Negative Regulators of Wnt-TCF Signaling Pathway -: Negative regulators of Wnt-TCF (canonical Wnt) signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Negative Regulators of Hedgehog Signaling Pathway -: Negative regulators of hedgehog signaling down-regulate the pathway, resulting in the repression of transcription of hh-responsive genes.
Positive Regulators of Hedgehog Signaling Pathway -: Positive regulators of hedgehog signaling up-regulate the pathway, resulting in the activation of transcription of hh-responsive genes.
Negative Regulators of Hippo Signaling Pathway -: The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-39

Cytogenetic map

11B7-11B8

Sequence location

X:12,653,626..12,657,834 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

11B7-11B8

Limits computationally determined from genome sequence between P{EP}CG11138^EP1313 and P{EP}EP1632&P{EP}EP1449

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

11B13-11B16

(determined by in situ hybridisation)

(Peter et al., 2002, Schäfer et al., 1999.12.14)

11B4-11B14

(Santos, 1996.1.4)

Experimentally Determined Recombination Data

Location

1-39

(FlyBase, 2016)

1-39.6

(Comeron et al., 2012)

(Santos, 1996.1.4)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Bloomington

55067

w^*; P{UAS-CkIα.HA}1/CyO

25786

y¹ v¹; P{TRiP.JF01792}attP2

12303

w^67c23 P{lacW}CkIα^G0492/FM7c

55068

w^*; P{UAS-CkIα.HA}5/TM3, Sb¹

41711

y¹ v¹; P{TRiP.HMS02276}attP40

17009

w¹¹¹⁸ P{EP}CkIα^EP1555 EP1555^EP1555

35153

y¹ sc^* v¹ sev²¹; P{TRiP.GL00021}attP2

57084

y¹ w^* CkIα^A P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

FlyORF

F000940

M{UAS-CkIα.ORF.3xHA}ZH-86Fb

VDRC

v110768

P{KK107574}VIE-260B

v13664

w¹¹¹⁸; P{GD4256}v13664

v342444

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01363}attP40/CyO-GFP

More stocks available...

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (219)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD05574

Other clones

DPiM_LD05574

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015024

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015024

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32221

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: CkIα CG2028

(Ke, 1999.11)

Source for database merge of

Source for merge of: CkIα l(1)G0492

(Peter et al., 2002)

Source for merge of: CkIα anon-WO03040301.93 anon-WO03040301.95

(FlyBase, 1996-)

Additional comments

Source for merge of CkIα anon-WO03040301.93 anon-WO03040301.95 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Other Comments

dsRNA made from templates generated with primers directed against CkIα profoundly increases the wg-signaling pathway.

(Seto and Bellen, 2006)

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

The dco and CkIα products act additively to provide optimal kinase activity required for efficient ci protein phosphorylation and processing.

(Jia et al., 2005)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of G1 phase cells and a a decrease in cell size is seen.

(Bettencourt-Dias et al., 2004)

CkIα phosphorylates arm and may stimulate its degradation.

(Yanagawa et al., 2002)

CkIα protein is isolated and characterised.

(Santos et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 80 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32221

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P54367

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JJI5

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015024

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015024

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2028

Flygut - An atlas of the Drosophila adult midgut

FBgn0015024

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

93695

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

32221

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034052

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG2028

modMine - A data warehouse for the modENCODE project

FBgn0015024

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P54367

Linkouts

BioGRID - A database of protein and genetic interactions.

58615

DPiM - Drosophila Protein interaction map

FBgn0015024

DroID - A comprehensive database of gene and protein interactions.

FBgn0015024

DRSC - Results frm RNAi screens

FBgn0015024

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015024

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG2028-RB

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015024

FlyMine - An integrated database for Drosophila genomics

FBgn0015024

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

32221

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

CkIalpha

MIST (protein-protein) - An integrated Molecular Interaction Database

CkIalpha

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (39)

Reported As

Symbol Synonym

CG2028

(Çiçek et al., 2016, Hosono et al., 2015, Fukuoh et al., 2014, Haelterman et al., 2014, Schertel et al., 2013, Ni et al., 2011.7.19, Buechling et al., 2010, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Perkins et al., 2009.1.15, Morrison et al., 2000)

CK I

(Weber et al., 2007, Behrens, 2000, Horiuchi et al., 2000)

CK1

(Zhao et al., 2017, Bejsovec, 2013, Spratford and Kumar, 2013, Zhou and Kalderon, 2011, Jia et al., 2009, Smelkinson et al., 2007, Tolwinski, 2007, Zhao et al., 2007, Smelkinson and Kalderon, 2006, Smelkinson and Kalderon, 2006, Kalderon, 2005, Torroja et al., 2005, Lum and Beachy, 2004, Ou et al., 2003, Price et al., 2003, Wharton, 2003, Lefers and Holmgren, 2002, Nybakken and Perrimon, 2002)

CK1a

(Buechling et al., 2011, Zhang et al., 2005)

CK1alpha

(Mbodj et al., 2013, Ding and Dale, 2002, Kalderon, 2002)

CK1α

(Li et al., 2016, Shi et al., 2014, Legent et al., 2012, Shi et al., 2011, Buechling et al., 2010, Galletti et al., 2009, Mennella et al., 2009, Bjorklund et al., 2006, Fearon and Cadigan, 2005, Lum et al., 2003)

CKI

(Zouaz et al., 2018, Xiong et al., 2015, Price et al., 2007, Tountas and Fortini, 2007, Heeg-Truesdell, 2006, Stadeli, 2006, Ishii, 2005, Bienz and Hamada, 2004, Jiang, 2002)

CKI α

(Takada et al., 2005)

CKIa

(Di Cara et al., 2015, Morrison et al., 2000)

CKIalpha

(Lindquist et al., 2011, Blanco et al., 2010)

CKIα

(Graves et al., 2020, Xu et al., 2020, Kanoh et al., 2015, Kim et al., 2014, Komori et al., 2014, Lindquist et al., 2011, Zhou and Kalderon, 2011, D'Ambrosio and Vale, 2010, Feng and Irvine, 2009, Strutt et al., 2006, Zhang et al., 2006, Jia et al., 2005, Jia et al., 2004, Matsubayashi et al., 2004, Bussell, 2003, Polakis, 2002, Yanagawa et al., 2002)

Ck1alpha

(Ugrankar et al., 2018)

Ck1α

(Tsai and Galko, 2019, Choi et al., 2014)

CkI α

(Yin et al., 2013)

CkIa

(Pospisilik et al., 2010, Peter et al., 2002)

CkIalpha

(Song et al., 2017, Ugrankar et al., 2015, Nybakken, 2014.2.28, Sopko et al., 2014, Bonke et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Schertel et al., 2013, Yamamoto et al., 2013-, Zhang et al., 2013, Friedman et al., 2011, Toku et al., 2011, Popodi et al., 2010-, Venken et al., 2010, Wasbrough et al., 2010, Giot et al., 2003)

CkIα

(Wang et al., 2020, Tan et al., 2016, Bouleau and Tricoire, 2015, Kanoh et al., 2015, Zang et al., 2015, Haelterman et al., 2014, Briscoe and Thérond, 2013, Kanao et al., 2012, Bai et al., 2007, Liu et al., 2007, Stroschein-Stevenson et al., 2006, Furlong, 2005, Nybakken et al., 2005, Zhang et al., 2005)

PKA-C

(Matsubayashi et al., 2004)

anon-WO03040301.93

anon-WO03040301.95

ck1a

(Seto and Bellen, 2006)

ck1α

(DasGupta et al., 2005)

ckI

(Fulford et al., 2019)

ckIα

(Fulford et al., 2019)

dmCK1

(Santos et al., 1996)

dmckI

(Santos, 1996.1.4)

l(1)G0492

(Schäfer et al., 1999.12.14)

Name Synonyms

Casein Kinase 1

(Stadeli, 2006, Apionishev et al., 2005, Torroja et al., 2005)

Casein Kinase 1α

(Legent et al., 2012)

Casein kinase 1

(Santos et al., 1996)

Casein kinase I

(Tountas and Fortini, 2007, Marques, 2005)

Casein kinase Ialpha

(Nybakken, 2014.2.28)

Casein kinase Iα

(Graves et al., 2020, Edwards et al., 2009, Cadigan, 2002)

caesin kinase I α

(D'Ambrosio and Vale, 2010)

casein kinase 1

(Smelkinson and Kalderon, 2006)

casein kinase 1a

(Seto and Bellen, 2006)

casein kinase I

(Bienz and Hamada, 2004)

casein kinase Iα

(Kanoh et al., 2015, Matsubayashi et al., 2004)

casein kinase-1

(Spratford and Kumar, 2013)

Secondary FlyBase IDs

FBgn0029043
FBgn0066807
FBgn0066808

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (182)

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