FlyBase Gene Report: Dmel\Cyp9b2

General Information

Symbol

Dmel\Cyp9b2

Species

D. melanogaster

Name

Cytochrome P450 9b2

Annotation Symbol

CG4486

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015039

Gene Model Status

Current

Stock Availability

3 publicly available

Gene Summary

May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides. (UniProt, Q9V4I1)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

P450

Key Links

Genomic Location

Cytogenetic map

42F2-42F2

Sequence location

2R:7,130,277..7,132,139 [-]

Recombination map

2-55

RefSeq locus

NT_033778 REGION:7130277..7132139

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (4 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables heme binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002403

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables iron ion binding

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002403

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables monooxygenase activity

inferred from electronic annotation with InterPro:IPR002403

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen

inferred from electronic annotation with InterPro:IPR001128, InterPro:IPR002403, InterPro:IPR017972

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Cellular Component (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

CYTOCHROME P450 - CYP3 CLAN

Protein Family (UniProt)

Belongs to the cytochrome P450 family. (Q9V4I1)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOCHROME P450 - CYP3 CLAN -: Cytochrome P450, or CYP genes, constitute one of the largest families of genes, with representatives in virtually all living organisms. P450 enzymes are heme-thiolate proteins best known for their monooxygenase role, catalyzing the transfer of one atom of molecular oxygen to a substrate and reducing the other to water. The CYP3 clan includes D. melanogaster CYP6, 9, 28, 308-310 and 317 family genes. Few CYP3 enzymes have been characterized, though many have been shown to metabolize xenobiotics and plant natural compounds. (Adapted from FBrf0248421.)

Protein Function (UniProtKB)

May be involved in the metabolism of insect hormones and in the breakdown of synthetic insecticides.

(UniProt, Q9V4I1)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Cyp9b2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V4I1)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.48

Gene model reviewed during 5.44

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Cyp9b2-RA

FBtr0089055

NM_078922

1667

505

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Cyp9b2-PA

FBpp0088126

58.9

505

8.35

NP_523646

AAF59290

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q9V4I1

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cyp9b2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cyp9b2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 7 -- 8

embryonic/larval midgut primordium

(Chung et al., 2009)

embryonic stage 13 -- 16

embryonic midgut chamber

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

third instar larval stage

embryonic/larval Malpighian tubule

(Chung et al., 2009)

embryonic/larval midgut

Comment: m1-2

(Chung et al., 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cyp9b2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cyp9b2

View exonic expression by tissue for Dmel\Cyp9b2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015039

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35635/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015039

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015039

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015039

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4486

Flygut - An atlas of the Drosophila adult midgut

FBgn0015039

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Cyp9b2

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 0 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 6 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cyp9b2

Transgenic constructs containing regulatory region of Cyp9b2

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene disrupted in

Df(2R)Exel6283

(Ryder, 2004.4.26)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (19)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\CYP3A5

7 of 15

Yes

6 of 15

6 of 15

5 of 15

4 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (26)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Cyp3a11

8 of 15

Yes

Mmus\Cyp3a16

8 of 15

Yes

Mmus\Cyp3a41a

8 of 15

Yes

Mmus\Cyp3a44

8 of 15

Yes

Mmus\Cyp3a25

7 of 15

Yes

Mmus\Cyp3a57

7 of 15

Yes

Mmus\Cyp3a59

7 of 15

Yes

Mmus\Cyp3a13

6 of 15

Mmus\Tbxas1

4 of 15

Mmus\Cyp3a41b

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (16)

Rnor\Cyp3a18

7 of 13

Yes

Rnor\Cyp3a9

6 of 13

Yes

Rnor\Cyp3a23/3a1

6 of 13

Yes

Rnor\Cyp3a2

5 of 13

Rnor\Cyp3a62

4 of 13

Yes

Rnor\LOC100910877

4 of 13

Yes

Rnor\Tbxas1

4 of 13

Rnor\Cyp3a71-ps

1 of 13

Yes

Rnor\Cyp3a73

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (7)

Xtro\cyp3a4

3 of 12

Yes

Xtro\cyp3a5

3 of 12

Yes

Xtro\LOC100494419

3 of 12

Yes

Xtro\tbxas1

3 of 12

Yes

Xtro\LOC100487054

1 of 12

Xtro\LOC100487665

1 of 12

Yes

Xtro\LOC100487846

1 of 12

Danio rerio (Zebrafish) (10)

Drer\cyp3c2

6 of 15

Yes

Drer\cyp3c3

6 of 15

Yes

Drer\cyp3a65

4 of 15

Yes

Drer\cyp3c1

4 of 15

Yes

Drer\cyp3c4

4 of 15

Yes

4 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (32)

Cele\cyp-25A1

7 of 15

Yes

Cele\cyp-25A2

7 of 15

Yes

Cele\cyp-25A3

7 of 15

Yes

Cele\cyp-25A4

7 of 15

Yes

Cele\cyp-13A1

6 of 15

Cele\cyp-13A3

6 of 15

Yes

6 of 15

6 of 15

6 of 15

6 of 15

Yes

Cele\cyp-13A10

6 of 15

Cele\cyp-13A11

6 of 15

Yes

Cele\cyp-13A12

6 of 15

Yes

5 of 15

5 of 15

5 of 15

5 of 15

5 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (27)

Atha\CYP711A1

4 of 9

Yes

3 of 9

3 of 9

3 of 9

3 of 9

3 of 9

3 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

2 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\ERG11

1 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\erg5

1 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (56)

Gene Symbol

Score

Source

Alignment

7 of 10

7 of 10

7 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

5 of 10

4 of 10

4 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CYP3A5; cytochrome P450 family 3 subfamily A member 5

model of essential hypertension

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CYP3A5; cytochrome P450 family 3 subfamily A member 5

7 of 15

605325

HYPERTENSION, ESSENTIAL

essential hypertension

CYP3A4; cytochrome P450 family 3 subfamily A member 4

6 of 15

124010

VITAMIN D-DEPENDENT RICKETS, TYPE 3; VDDR3

CYP3A43; cytochrome P450 family 3 subfamily A member 43

6 of 15

606534

CYP3A7-CYP3A51P; CYP3A7-CYP3A51P readthrough

5 of 15

TBXAS1; thromboxane A synthase 1

4 of 15

274180

GHOSAL HEMATODIAPHYSEAL DYSPLASIA; GHDD

Ghosal hematodiaphyseal syndrome

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Cyp9b2 - boca

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61523

DroID - A comprehensive database of gene and protein interactions.

FBgn0015039

MIST (protein-protein) - An integrated Molecular Interaction Database

Cyp9b2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-55

Cytogenetic map

42F2-42F2

Sequence location

2R:7,130,277..7,132,139 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

42F2-42F2

Limits computationally determined from genome sequence between P{lacW}vimar^k16722 and P{lacW}cos^k16101

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

43A1-43A2

(Dunkov, 2000.6.29)

42D1-42D2

(determined by in situ hybridisation)

(Dunkov et al., 1996)

Experimentally Determined Recombination Data

Location

2-55

(FlyBase, 2016)

2-56.3

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (3)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

62996

y¹ v¹; P{TRiP.HMJ30073}attP40

91840

y¹ sc^* v¹ sev²¹; P{TOE.GS01702}attP40

VDRC

v102496

P{KK111822}VIE-260B

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (79)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH08116

Other clones

DPiM_GH08116

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015039

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015039

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35635

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: Cyp9b2 BEST:GH08116

(FlyBase, 1996-)

Additional comments

The genes Cyp9b2 and Cyp9b1 share >70% identity and close proximity, and so may have been derived from a DNA-based tandem duplication.

(Langille and Clark, 2007)

Source for merge of Cyp9b2 BEST:GH08116 was a shared cDNA ( date:030206 ).

(FlyBase, 1996-)

Other Comments

Identified molecularly using degenerate PCR primers designed from conserved regions of known insect P450 genes.

(Dunkov et al., 1996)

The adenylate cyclase inactivator forskolin and its pharmacologically inactive derivative 1,9-dideoxyforskolin inhibit, in a dose-dependent fashion, the ecdysone 20-monooxygenase activity associated with wandering stage larvae.

(Keogh et al., 1992)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35635

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V4I1

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V4I1

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V4I1

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015039

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015039

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35635/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015039

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cyp9b2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015039

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015039

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4486

Flygut - An atlas of the Drosophila adult midgut

FBgn0015039

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35635

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG4486

MARRVEL_MODEL - MARRVEL (model organism gene)

35635

modMine - A data warehouse for the modENCODE project

FBgn0015039

Linkouts

BioGRID - A database of protein and genetic interactions.

61523

DroID - A comprehensive database of gene and protein interactions.

FBgn0015039

DRSC - Results frm RNAi screens

FBgn0015039

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015039

FlyMine - An integrated database for Drosophila genomics

FBgn0015039

MIST (protein-protein) - An integrated Molecular Interaction Database

Cyp9b2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

9b2

(Nelson, 2001.2.5)

BEST:GH08116

CG4486

(Parisi et al., 2010, Pessa et al., 2010, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009)

Cyp9B2

(Li et al., 2008)

Cyp9b2

(Scanlan et al., 2020, Maitra et al., 2019, Ruppert et al., 2017, Transgenic RNAi Project members, 2017-, Trienens et al., 2017, Good et al., 2014, Peters et al., 2013, Stefanatos et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Niwa et al., 2011, Giraudo et al., 2010, Kong et al., 2010, Pessa et al., 2010, Liu and Lehmann, 2008, Lai et al., 2007, Langille and Clark, 2007, Harbison et al., 2005)

P-450

(Danielson et al., 1995)

P-450 Cyp9b2

(Neal et al., 2006)

P450

(Arking, 1998, Zakharenko and Zakharov, 1998, Frolich and Wurgler, 1991)

cyp9b2

(Ceriani et al., 2002)

Name Synonyms

Cytochrome P450 9b2

Cytochrome P450-9b2

(Radyuk et al., 2012, Harbison et al., 2005)