eIF4E, eIF-4E, eIF4E-1, eIF4E-2, deIF4E
binds to the mRNA 5' cap thus controlling a crucial step in translation initiation - required for cell growth - promotes dedifferentiation of neuroblasts back to a stem cell-like state thus functioning as an oncogene - a target of Ago2 in translational repression - functions as a splice factor for and pre-mRNAs
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E1 and eIF4G1. eIF4E1 is also known to interact with other partners (By similarity). Recruited by cup in oocytes and in early embryos, preventing the interaction with eIF4G. The interaction with cup therefore prevents the translation of key transcripts such as oskar (osk) and nanos (nos) in some regions in the early embryo (PubMed:14691132, PubMed:14723848, PubMed:14685270, PubMed:26294658). Interacts with mxt (PubMed:23716590, PubMed:26294658). Interacts with 4E-T and Thor (PubMed:26294658). Forms a RNP containing at least me31B, eIF4E1, cup, tral and pAbp; this interaction is required for the translational silencing of maternal mRNAs during the maternal-to-zygotic transition (PubMed:28875934).
Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the eIF4F complex.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4E1 using the Feature Mapper tool.
eIF-4E transcripts are expressed throughout embryogenesis in all cells. They accumulate at higher levels in some tissues at certain developmental stages. Transcripts preferentially accumulate in the pole cells from early stages up through germ band extension. Transcripts accumulate at high levels in the endodermal primordia that will give rise to the anterior and posterior midguts as well as in the mesodermal layer and the salivary gland primordia. At later stages, high expression is observed in the endodermal layer of the gut and in th somatic muscle.
The 1.4kb eIF-4E transcript is lower in abundance than the larger two in early embryos.
eIF4E1 protein is expressed during many stages of spermatogenesis, both in the germ cells and surrounding somatic cyst cells. eIF4E1 protein is detected in the hub cells containing the stem cell niche at the apical tip of the testes, in spermatogonia, primary and secondary spermatocytes,and in spermatids in the early stages elongation. eIF4E1 protein is not detected in mature elongated spermatid bundles undergoing differentiation, but is detected in surrounding somatic cyst cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\eIF4E1 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: eIF4E1 eIF-4E
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
dsRNA made from templates generated with primers directed against this gene reduces mean cell diameter. Silencing both eIF-4E and S6k together does not reduce mean cell diameter to the same extent as rapamycin treatment.
The phosphorylation of eIF-4E at Ser251 is biologically significant and is essential for normal growth and development.
Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.
Cloning and sequencing of eIF-4E revealed the existance of three mRNAs that are generated by alternative splicing of a primary transcript and all have different 5' untranslated leader regions. eIF-4E is a single copy gene. Expression of eIF-4E is spatially and temporally controlled during embryonic development. eIF-4E is ubiquitously expressed during embryogenesis but transcripts preferentially accumulate in certain tissues, particularly in the pole cells at different developmental stages.
eIF-4E protein from both control and heat shocked cells has been purified and characterised.
Isolated from a 0 to 20 hour embryo cDNA library using a polyclonal antibody against eIF-4E.
An eIF-4E cDNA has been cloned and sequenced, and its expression pattern has been analysed.
Drosophila elongation factor 4F consists of two subunits, a polypeptide of about 200kD and the product of eIF-4E, the cap-binding protein.