Gene Dmel\eIF-4E

(BDGP Project Members, 1994-1999)

67A-67A

(Lachance et al., 1996)

67B1-67B2

Experimentally Determined Recombination Data

Location

(Lachance et al., 1996, Lasko, 1996.4.9)

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\eIF-4E for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.46

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

eIF-4E-RA

FBtr0076490

NM_168334

1430

259

eIF-4E-RB

FBtr0076487

NM_080090

1484

259

eIF-4E-RC

FBtr0076488

NM_168333

1156

248

eIF-4E-RD

FBtr0076489

NM_168335

1723

259

eIF-4E-RE

FBtr0076492

NM_168336

1524

259

eIF-4E-RF

FBtr0076493

NM_168337

1452

259

eIF-4E-RG

FBtr0076491

NM_168338

1358

259

eIF-4E-RH

FBtr0333884

NM_001274697

1492

259

eIF-4E-RI

FBtr0333885

NM_001274698

1793

259

Additional Transcript Data & Comments

Reported size (kB)

2.0, 1.7, 1.4 (northern blot)

2.0, 1.5, 1.2 (northern blot)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

eIF-4E-PA

FBpp0076218

29.2

259

6.17

NP_729481

AAF50283

eIF-4E-PB

FBpp0076215

29.2

259

6.17

NP_524829

AAF50282

eIF-4E-PC

FBpp0076216

27.8

248

5.04

NP_729480

AAF50281

eIF-4E-PD

FBpp0076217

29.2

259

6.17

NP_729482

AAN11963

eIF-4E-PE

FBpp0076220

29.2

259

6.17

NP_729483

AAN11964

eIF-4E-PF

FBpp0076221

29.2

259

6.17

NP_729484

AAN11965

eIF-4E-PG

FBpp0076219

29.2

259

6.17

NP_729485

AAN11966

eIF-4E-PH

FBpp0306016

29.2

259

6.17

NP_001261626

AGB94321

eIF-4E-PI

FBpp0306017

29.2

259

6.17

NP_001261627

AGB94322

Additional Polypeptide Data & Comments

Reported size (kDa)

259 (aa); 29 (kD)

259, 248 (aa)

248 (aa)

(Maroto and Sierra, 1989)

35 (kD)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Translation Initiation factor eIF- 4e (IPR001040)

Eukaryotic translation initiation factor 4E (eIF-4E), conserved site (IPR019770)

Translation Initiation factor eIF- 4e-like domain (IPR023398)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P48598

UniProtKB/TrEMBL

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | ubiquitous

embryonic/larval somatic muscle

embryonic/larval salivary gland

embryonic/larval digestive system

posterior midgut primordium

anterior midgut primordium

pole cell

embryonic stage 1 -- 3

organism

Comment:maternally deposited

embryonic stage 4 -- 6

organism | ubiquitous

pole cell

embryonic stage 7 -- 8

organism | ubiquitous

embryonic stage 9 -- 10

organism | faint & ubiquitous

organism | ubiquitous

embryonic stage 11 -- 12

anterior midgut primordium

dorsal epidermis primordium

hindgut proper primordium

organism | ubiquitous

posterior midgut primordium

ventral epidermis primordium

embryonic stage 13 -- 16

embryonic dorsal epidermis

embryonic foregut

embryonic hindgut

embryonic ventral epidermis

embryonic/larval midgut

organism | ubiquitous

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

Additional Descriptive Data

The 1.4kb eIF-4E transcript is lower in abundance than the larger two in early embryos.

eIF-4E transcripts are expressed throughout embryogenesis in all cells. They accumulate at higher levels in some tissues at certain developmental stages. Transcripts preferentially accumulate in the pole cells from early stages up through germ band extension. Transcripts accumulate at high levels in the endodermal primordia that will give rise to the anterior and posterior midguts as well as in the mesodermal layer and the salivary gland primordia. At later stages, high expression is observed in the endodermal layer of the gut and in th somatic muscle.

eIF-4E transcripts are detected throughout development on northern blots.

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

first instar larval stage

organism

pupal stage

organism

adult stage | male

testis

adult stage | female

ovary

Additional Descriptive Data

eIF-4E protein is expressed in all tissues analyzed.

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(Sigrist et al., 2000, Ottone et al., 2011)

References

cytoplasm

inferred from direct assay

cytoplasmic mRNA processing body

inferred from direct assay

(Liu and Gall, 2007)

eukaryotic translation initiation factor 4F complex

inferred from direct assay

inferred from physical interaction with eIF4G

microtubule associated complex

inferred from direct assay

(Hughes et al., 2008)

postsynaptic density

inferred from direct assay

(Menon et al., 2004)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1322.45
Larval Midgut		842.3
Larval Hindgut		1025.1
Larval Malpighian Tubules		962.9
Larval Fat Body		714.4
Larval Salivary Gland		1838.1
Larval Trachea		1170.875
Larval Carcass		1270.625
Adult Head		805
Adult Eye		1041.775
Adult Brain		1070.2
Adult Thoracic-Abdominal Ganglion		1238.1
Adult Crop		1023.1
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		916
Adult Fat Body		870.2
Adult Salivary Gland		1301.8
Adult Heart		806.5
Adult VirginFemale Spermatheca		1616.3
Adult InseminatedFemale Spermatheca		1809.8
Adult Ovary		1605.4
Adult Testis		814.5
Adult Male Accessory Gland		1631.7
Adult Carcass		707
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1322.45)
Larval Midgut		(842.3)
Larval Hindgut		(1025.1)
Larval Malpighian Tubules		(962.9)
Larval Fat Body		(714.4)
Larval Salivary Gland		(1838.1)
Larval Trachea		(1170.875)
Larval Carcass		(1270.625)
Adult Head		(805)
Adult Eye		(1041.775)
Adult Brain		(1070.2)
Adult Thoracic-Abdominal Ganglion		(1238.1)
Adult Crop		(1023.1)
Adult Midgut		(643.6)
Adult Hindgut		(662.1)
Adult Malpighian Tubules		(916)
Adult Fat Body		(870.2)
Adult Salivary Gland		(1301.8)
Adult Heart		(806.5)
Adult VirginFemale Spermatheca		(1616.3)
Adult InseminatedFemale Spermatheca		(1809.8)
Adult Ovary		(1605.4)
Adult Testis		(814.5)
Adult Male Accessory Gland		(1631.7)
Adult Carcass		(707)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(1322.45)
Larval Midgut		842.3
Larval Hindgut		1025.1
Larval Malpighian Tubules		962.9
Larval Fat Body		714.4
Larval Salivary Gland		(1838.1)
Larval Trachea		(1170.875)
Larval Carcass		(1270.625)
Adult Head		805
Adult Eye		1041.775
Adult Brain		1070.2
Adult Thoracic-Abdominal Ganglion		(1238.1)
Adult Crop		1023.1
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		916
Adult Fat Body		870.2
Adult Salivary Gland		(1301.8)
Adult Heart		806.5
Adult VirginFemale Spermatheca		(1616.3)
Adult InseminatedFemale Spermatheca		(1809.8)
Adult Ovary		(1605.4)
Adult Testis		814.5
Adult Male Accessory Gland		(1631.7)
Adult Carcass		707
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1322.45
Larval Midgut		842.3
Larval Hindgut		1025.1
Larval Malpighian Tubules		962.9
Larval Fat Body		714.4
Larval Salivary Gland		1838.1
Larval Trachea		1170.875
Larval Carcass		1270.625
Adult Head		805
Adult Eye		1041.775
Adult Brain		1070.2
Adult Thoracic-Abdominal Ganglion		1238.1
Adult Crop		1023.1
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		916
Adult Fat Body		870.2
Adult Salivary Gland		1301.8
Adult Heart		806.5
Adult VirginFemale Spermatheca		1616.3
Adult InseminatedFemale Spermatheca		1809.8
Adult Ovary		1605.4
Adult Testis		814.5
Adult Male Accessory Gland		1631.7
Adult Carcass		707
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		1322.45
Larval Midgut		842.3
Larval Hindgut		1025.1
Larval Malpighian Tubules		962.9
Larval Fat Body		714.4
Larval Salivary Gland		1838.1
Larval Trachea		1170.875
Larval Carcass		1270.625
Adult Head		805
Adult Eye		1041.775
Adult Brain		1070.2
Adult Thoracic-Abdominal Ganglion		1238.1
Adult Crop		1023.1
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		916
Adult Fat Body		870.2
Adult Salivary Gland		1301.8
Adult Heart		806.5
Adult VirginFemale Spermatheca		1616.3
Adult InseminatedFemale Spermatheca		1809.8
Adult Ovary		1605.4
Adult Testis		814.5
Adult Male Accessory Gland		1631.7
Adult Carcass		707
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(1322.45)
Larval Midgut		(842.3)
Larval Hindgut		(1025.1)
Larval Malpighian Tubules		(962.9)
Larval Fat Body		714.4
Larval Salivary Gland		(1838.1)
Larval Trachea		(1170.875)
Larval Carcass		(1270.625)
Adult Head		805
Adult Eye		(1041.775)
Adult Brain		(1070.2)
Adult Thoracic-Abdominal Ganglion		(1238.1)
Adult Crop		(1023.1)
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		(916)
Adult Fat Body		(870.2)
Adult Salivary Gland		(1301.8)
Adult Heart		806.5
Adult VirginFemale Spermatheca		(1616.3)
Adult InseminatedFemale Spermatheca		(1809.8)
Adult Ovary		(1605.4)
Adult Testis		(814.5)
Adult Male Accessory Gland		(1631.7)
Adult Carcass		707
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		1322.45
Larval Midgut		842.3
Larval Hindgut		1025.1
Larval Malpighian Tubules		962.9
Larval Fat Body		714.4
Larval Salivary Gland		(1838.1)
Larval Trachea		1170.875
Larval Carcass		1270.625
Adult Head		805
Adult Eye		1041.775
Adult Brain		1070.2
Adult Thoracic-Abdominal Ganglion		1238.1
Adult Crop		1023.1
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		916
Adult Fat Body		870.2
Adult Salivary Gland		1301.8
Adult Heart		806.5
Adult VirginFemale Spermatheca		1616.3
Adult InseminatedFemale Spermatheca		(1809.8)
Adult Ovary		1605.4
Adult Testis		814.5
Adult Male Accessory Gland		1631.7
Adult Carcass		707
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		1322.45
Larval Midgut		842.3
Larval Hindgut		1025.1
Larval Malpighian Tubules		962.9
Larval Fat Body		714.4
Larval Salivary Gland		1838.1
Larval Trachea		1170.875
Larval Carcass		1270.625
Adult Head		805
Adult Eye		1041.775
Adult Brain		1070.2
Adult Thoracic-Abdominal Ganglion		1238.1
Adult Crop		1023.1
Adult Midgut		643.6
Adult Hindgut		662.1
Adult Malpighian Tubules		916
Adult Fat Body		870.2
Adult Salivary Gland		1301.8
Adult Heart		806.5
Adult VirginFemale Spermatheca		1616.3
Adult InseminatedFemale Spermatheca		1809.8
Adult Ovary		1605.4
Adult Testis		814.5
Adult Male Accessory Gland		1631.7
Adult Carcass		707
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	805
NA	Eye	1041.775
NA	Brain	1070.2
1322.45	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	1238.1
NA	Crop	1023.1
842.3	Midgut	643.6
1025.1	Hindgut	662.1
962.9	Malpighian Tubules	916
714.4	Fat Body	870.2
1838.1	Salivary Gland	1301.8
NA	Heart	806.5
1170.875	Trachea	NA
NA	VirginFemale Spermatheca	1616.3
NA	InseminatedFemale Spermatheca	1809.8
NA	Ovary	1605.4
NA	Testis	814.5
NA	Male Accessory Gland	1631.7
1270.625	Carcass	707

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		373
central nervous system, larvae L3		263
central nervous system, pupae P8		170
head, virgin 1-day female		97
head, virgin 4-day female		101
head, virgin 20-day female		97
head, mated 1-day female		94
head, mated 4-day female		79
head, mated 20-day female		92
head, mated 1-day male		119
head, mated 4-day male		111
head, mated 20-day male		124
salivary gland, larvae L3 wandering		94
salivary gland, white prepupae		215
digestive system, larvae L3 wandering		154
digestive system, 1-day adult		92
digestive system, 4-day adult		108
digestive system, 20-day adult		82
fat body, larvae L3 wandering		65
fat body, white prepupae		126
fat body, pupae P8		245
carcass, larvae L3 wandering		218
carcass, 1-day adult		79
carcass, 4-day adult		102
carcass, 20-day adult		111
ovary, virgin 4-day female		558
ovary, mated 4-day female		552
testis, mated 4-day male		201
accessory gland, mated 4-day male		90
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(373)
central nervous system, larvae L3		(263)
central nervous system, pupae P8		(170)
head, virgin 1-day female		(97)
head, virgin 4-day female		(101)
head, virgin 20-day female		(97)
head, mated 1-day female		(94)
head, mated 4-day female		(79)
head, mated 20-day female		(92)
head, mated 1-day male		(119)
head, mated 4-day male		(111)
head, mated 20-day male		(124)
salivary gland, larvae L3 wandering		(94)
salivary gland, white prepupae		(215)
digestive system, larvae L3 wandering		(154)
digestive system, 1-day adult		(92)
digestive system, 4-day adult		(108)
digestive system, 20-day adult		(82)
fat body, larvae L3 wandering		(65)
fat body, white prepupae		(126)
fat body, pupae P8		(245)
carcass, larvae L3 wandering		(218)
carcass, 1-day adult		(79)
carcass, 4-day adult		(102)
carcass, 20-day adult		(111)
ovary, virgin 4-day female		(558)
ovary, mated 4-day female		(552)
testis, mated 4-day male		(201)
accessory gland, mated 4-day male		(90)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(373)
central nervous system, larvae L3		(263)
central nervous system, pupae P8		(170)
head, virgin 1-day female		97
head, virgin 4-day female		101
head, virgin 20-day female		97
head, mated 1-day female		94
head, mated 4-day female		79
head, mated 20-day female		92
head, mated 1-day male		(119)
head, mated 4-day male		(111)
head, mated 20-day male		(124)
salivary gland, larvae L3 wandering		94
salivary gland, white prepupae		(215)
digestive system, larvae L3 wandering		(154)
digestive system, 1-day adult		92
digestive system, 4-day adult		(108)
digestive system, 20-day adult		82
fat body, larvae L3 wandering		65
fat body, white prepupae		(126)
fat body, pupae P8		(245)
carcass, larvae L3 wandering		(218)
carcass, 1-day adult		79
carcass, 4-day adult		102
carcass, 20-day adult		(111)
ovary, virgin 4-day female		(558)
ovary, mated 4-day female		(552)
testis, mated 4-day male		(201)
accessory gland, mated 4-day male		90
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		373
central nervous system, larvae L3		263
central nervous system, pupae P8		170
head, virgin 1-day female		97
head, virgin 4-day female		101
head, virgin 20-day female		97
head, mated 1-day female		94
head, mated 4-day female		79
head, mated 20-day female		92
head, mated 1-day male		119
head, mated 4-day male		111
head, mated 20-day male		124
salivary gland, larvae L3 wandering		94
salivary gland, white prepupae		215
digestive system, larvae L3 wandering		154
digestive system, 1-day adult		92
digestive system, 4-day adult		108
digestive system, 20-day adult		82
fat body, larvae L3 wandering		65
fat body, white prepupae		126
fat body, pupae P8		245
carcass, larvae L3 wandering		218
carcass, 1-day adult		79
carcass, 4-day adult		102
carcass, 20-day adult		111
ovary, virgin 4-day female		558
ovary, mated 4-day female		552
testis, mated 4-day male		201
accessory gland, mated 4-day male		90
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		373
central nervous system, larvae L3		263
central nervous system, pupae P8		170
head, virgin 1-day female		97
head, virgin 4-day female		101
head, virgin 20-day female		97
head, mated 1-day female		94
head, mated 4-day female		79
head, mated 20-day female		92
head, mated 1-day male		119
head, mated 4-day male		111
head, mated 20-day male		124
salivary gland, larvae L3 wandering		94
salivary gland, white prepupae		215
digestive system, larvae L3 wandering		154
digestive system, 1-day adult		92
digestive system, 4-day adult		108
digestive system, 20-day adult		82
fat body, larvae L3 wandering		65
fat body, white prepupae		126
fat body, pupae P8		245
carcass, larvae L3 wandering		218
carcass, 1-day adult		79
carcass, 4-day adult		102
carcass, 20-day adult		111
ovary, virgin 4-day female		558
ovary, mated 4-day female		552
testis, mated 4-day male		201
accessory gland, mated 4-day male		90
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(373)
central nervous system, larvae L3		(263)
central nervous system, pupae P8		(170)
head, virgin 1-day female		(97)
head, virgin 4-day female		(101)
head, virgin 20-day female		(97)
head, mated 1-day female		(94)
head, mated 4-day female		(79)
head, mated 20-day female		(92)
head, mated 1-day male		(119)
head, mated 4-day male		(111)
head, mated 20-day male		(124)
salivary gland, larvae L3 wandering		(94)
salivary gland, white prepupae		(215)
digestive system, larvae L3 wandering		(154)
digestive system, 1-day adult		(92)
digestive system, 4-day adult		(108)
digestive system, 20-day adult		(82)
fat body, larvae L3 wandering		(65)
fat body, white prepupae		(126)
fat body, pupae P8		(245)
carcass, larvae L3 wandering		(218)
carcass, 1-day adult		(79)
carcass, 4-day adult		(102)
carcass, 20-day adult		(111)
ovary, virgin 4-day female		(558)
ovary, mated 4-day female		(552)
testis, mated 4-day male		(201)
accessory gland, mated 4-day male		(90)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(373)
central nervous system, larvae L3		(263)
central nervous system, pupae P8		(170)
head, virgin 1-day female		97
head, virgin 4-day female		101
head, virgin 20-day female		97
head, mated 1-day female		94
head, mated 4-day female		79
head, mated 20-day female		92
head, mated 1-day male		119
head, mated 4-day male		111
head, mated 20-day male		124
salivary gland, larvae L3 wandering		94
salivary gland, white prepupae		(215)
digestive system, larvae L3 wandering		(154)
digestive system, 1-day adult		92
digestive system, 4-day adult		108
digestive system, 20-day adult		82
fat body, larvae L3 wandering		65
fat body, white prepupae		126
fat body, pupae P8		(245)
carcass, larvae L3 wandering		(218)
carcass, 1-day adult		79
carcass, 4-day adult		102
carcass, 20-day adult		111
ovary, virgin 4-day female		(558)
ovary, mated 4-day female		(552)
testis, mated 4-day male		(201)
accessory gland, mated 4-day male		90
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		373
central nervous system, larvae L3		263
central nervous system, pupae P8		170
head, virgin 1-day female		97
head, virgin 4-day female		101
head, virgin 20-day female		97
head, mated 1-day female		94
head, mated 4-day female		79
head, mated 20-day female		92
head, mated 1-day male		119
head, mated 4-day male		111
head, mated 20-day male		124
salivary gland, larvae L3 wandering		94
salivary gland, white prepupae		215
digestive system, larvae L3 wandering		154
digestive system, 1-day adult		92
digestive system, 4-day adult		108
digestive system, 20-day adult		82
fat body, larvae L3 wandering		65
fat body, white prepupae		126
fat body, pupae P8		245
carcass, larvae L3 wandering		218
carcass, 1-day adult		79
carcass, 4-day adult		102
carcass, 20-day adult		111
ovary, virgin 4-day female		558
ovary, mated 4-day female		552
testis, mated 4-day male		201
accessory gland, mated 4-day male		90
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of high expression. Peak expression observed within 00-06 and 12-18 hour embryonic stages, at stages throughout the pupal period, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		275
embryo 02-04hr		70
embryo 04-06hr		69
embryo 06-08hr		89
embryo 08-10hr		110
embryo 10-12hr		120
embryo 12-14hr		138
embryo 14-16hr		87
embryo 16-18hr		100
embryo 18-20hr		93
embryo 20-22hr		79
embryo 22-24hr		83
larva L1		108
larva L2		120
larva L3 12hr old		100
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		94
larva L3 puffstage 7-9		115
white prepupae new		118
white prepupae 12hr		130
white prepupae 24hr		138
pupae 2d postWPP		143
pupae 3d postWPP		127
pupae 4d postWPP		67
adult male 01day		78
adult male 05day		78
adult male 30day		66
adult female 01day		129
adult female 05day		244
adult female 30day		232
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(275)
embryo 02-04hr		(70)
embryo 04-06hr		(69)
embryo 06-08hr		(89)
embryo 08-10hr		(110)
embryo 10-12hr		(120)
embryo 12-14hr		(138)
embryo 14-16hr		(87)
embryo 16-18hr		(100)
embryo 18-20hr		(93)
embryo 20-22hr		(79)
embryo 22-24hr		(83)
larva L1		(108)
larva L2		(120)
larva L3 12hr old		(100)
larva L3 puffstage 1-2		(82)
larva L3 puffstage 3-6		(94)
larva L3 puffstage 7-9		(115)
white prepupae new		(118)
white prepupae 12hr		(130)
white prepupae 24hr		(138)
pupae 2d postWPP		(143)
pupae 3d postWPP		(127)
pupae 4d postWPP		(67)
adult male 01day		(78)
adult male 05day		(78)
adult male 30day		(66)
adult female 01day		(129)
adult female 05day		(244)
adult female 30day		(232)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(275)
embryo 02-04hr		70
embryo 04-06hr		69
embryo 06-08hr		89
embryo 08-10hr		(110)
embryo 10-12hr		(120)
embryo 12-14hr		(138)
embryo 14-16hr		87
embryo 16-18hr		100
embryo 18-20hr		93
embryo 20-22hr		79
embryo 22-24hr		83
larva L1		(108)
larva L2		(120)
larva L3 12hr old		100
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		94
larva L3 puffstage 7-9		(115)
white prepupae new		(118)
white prepupae 12hr		(130)
white prepupae 24hr		(138)
pupae 2d postWPP		(143)
pupae 3d postWPP		(127)
pupae 4d postWPP		67
adult male 01day		78
adult male 05day		78
adult male 30day		66
adult female 01day		(129)
adult female 05day		(244)
adult female 30day		(232)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		275
embryo 02-04hr		70
embryo 04-06hr		69
embryo 06-08hr		89
embryo 08-10hr		110
embryo 10-12hr		120
embryo 12-14hr		138
embryo 14-16hr		87
embryo 16-18hr		100
embryo 18-20hr		93
embryo 20-22hr		79
embryo 22-24hr		83
larva L1		108
larva L2		120
larva L3 12hr old		100
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		94
larva L3 puffstage 7-9		115
white prepupae new		118
white prepupae 12hr		130
white prepupae 24hr		138
pupae 2d postWPP		143
pupae 3d postWPP		127
pupae 4d postWPP		67
adult male 01day		78
adult male 05day		78
adult male 30day		66
adult female 01day		129
adult female 05day		244
adult female 30day		232
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		275
embryo 02-04hr		70
embryo 04-06hr		69
embryo 06-08hr		89
embryo 08-10hr		110
embryo 10-12hr		120
embryo 12-14hr		138
embryo 14-16hr		87
embryo 16-18hr		100
embryo 18-20hr		93
embryo 20-22hr		79
embryo 22-24hr		83
larva L1		108
larva L2		120
larva L3 12hr old		100
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		94
larva L3 puffstage 7-9		115
white prepupae new		118
white prepupae 12hr		130
white prepupae 24hr		138
pupae 2d postWPP		143
pupae 3d postWPP		127
pupae 4d postWPP		67
adult male 01day		78
adult male 05day		78
adult male 30day		66
adult female 01day		129
adult female 05day		244
adult female 30day		232
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(275)
embryo 02-04hr		(70)
embryo 04-06hr		(69)
embryo 06-08hr		(89)
embryo 08-10hr		(110)
embryo 10-12hr		(120)
embryo 12-14hr		(138)
embryo 14-16hr		(87)
embryo 16-18hr		(100)
embryo 18-20hr		(93)
embryo 20-22hr		(79)
embryo 22-24hr		(83)
larva L1		(108)
larva L2		(120)
larva L3 12hr old		(100)
larva L3 puffstage 1-2		(82)
larva L3 puffstage 3-6		(94)
larva L3 puffstage 7-9		(115)
white prepupae new		(118)
white prepupae 12hr		(130)
white prepupae 24hr		(138)
pupae 2d postWPP		(143)
pupae 3d postWPP		(127)
pupae 4d postWPP		(67)
adult male 01day		(78)
adult male 05day		(78)
adult male 30day		(66)
adult female 01day		(129)
adult female 05day		(244)
adult female 30day		(232)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(275)
embryo 02-04hr		70
embryo 04-06hr		69
embryo 06-08hr		89
embryo 08-10hr		110
embryo 10-12hr		120
embryo 12-14hr		138
embryo 14-16hr		87
embryo 16-18hr		100
embryo 18-20hr		93
embryo 20-22hr		79
embryo 22-24hr		83
larva L1		108
larva L2		120
larva L3 12hr old		100
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		94
larva L3 puffstage 7-9		115
white prepupae new		118
white prepupae 12hr		130
white prepupae 24hr		138
pupae 2d postWPP		143
pupae 3d postWPP		127
pupae 4d postWPP		67
adult male 01day		78
adult male 05day		78
adult male 30day		66
adult female 01day		129
adult female 05day		(244)
adult female 30day		(232)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		275
embryo 02-04hr		70
embryo 04-06hr		69
embryo 06-08hr		89
embryo 08-10hr		110
embryo 10-12hr		120
embryo 12-14hr		138
embryo 14-16hr		87
embryo 16-18hr		100
embryo 18-20hr		93
embryo 20-22hr		79
embryo 22-24hr		83
larva L1		108
larva L2		120
larva L3 12hr old		100
larva L3 puffstage 1-2		82
larva L3 puffstage 3-6		94
larva L3 puffstage 7-9		115
white prepupae new		118
white prepupae 12hr		130
white prepupae 24hr		138
pupae 2d postWPP		143
pupae 3d postWPP		127
pupae 4d postWPP		67
adult male 01day		78
adult male 05day		78
adult male 30day		66
adult female 01day		129
adult female 05day		244
adult female 30day		232
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		177
Schneider line 2 Sg4		101
embryonic 1182-4H		180
embryonic GM2		112
embryonic Kc167		145
embryonic S1		257
embryonic S3		183
leg disc CME L1		193
wing disc CME-W2		143
wing disc ML-DmD8		201
wing disc ML-DmD9		273
wing disc ML-DmD16-c3		160
wing disc ML-DmD21		128
wing disc ML-DmD32		163
haltere disc ML-DmD17-c3		207
eye-antennal disc ML-DmD11		214
antennal disc ML-DmD20-c5		149
mixed discs ML-DmD4-c1		171
CNS ML-DmBG1-c1		160
CNS ML-DmBG2-c2		156
tumorous blood cells mbn2		152
ovary fGS/OSS		156
ovary OSC		288
ovary OSS		150
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(177)
Schneider line 2 Sg4		(101)
embryonic 1182-4H		(180)
embryonic GM2		(112)
embryonic Kc167		(145)
embryonic S1		(257)
embryonic S3		(183)
leg disc CME L1		(193)
wing disc CME-W2		(143)
wing disc ML-DmD8		(201)
wing disc ML-DmD9		(273)
wing disc ML-DmD16-c3		(160)
wing disc ML-DmD21		(128)
wing disc ML-DmD32		(163)
haltere disc ML-DmD17-c3		(207)
eye-antennal disc ML-DmD11		(214)
antennal disc ML-DmD20-c5		(149)
mixed discs ML-DmD4-c1		(171)
CNS ML-DmBG1-c1		(160)
CNS ML-DmBG2-c2		(156)
tumorous blood cells mbn2		(152)
ovary fGS/OSS		(156)
ovary OSC		(288)
ovary OSS		(150)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(177)
Schneider line 2 Sg4		101
embryonic 1182-4H		(180)
embryonic GM2		(112)
embryonic Kc167		(145)
embryonic S1		(257)
embryonic S3		(183)
leg disc CME L1		(193)
wing disc CME-W2		(143)
wing disc ML-DmD8		(201)
wing disc ML-DmD9		(273)
wing disc ML-DmD16-c3		(160)
wing disc ML-DmD21		(128)
wing disc ML-DmD32		(163)
haltere disc ML-DmD17-c3		(207)
eye-antennal disc ML-DmD11		(214)
antennal disc ML-DmD20-c5		(149)
mixed discs ML-DmD4-c1		(171)
CNS ML-DmBG1-c1		(160)
CNS ML-DmBG2-c2		(156)
tumorous blood cells mbn2		(152)
ovary fGS/OSS		(156)
ovary OSC		(288)
ovary OSS		(150)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		177
Schneider line 2 Sg4		101
embryonic 1182-4H		180
embryonic GM2		112
embryonic Kc167		145
embryonic S1		257
embryonic S3		183
leg disc CME L1		193
wing disc CME-W2		143
wing disc ML-DmD8		201
wing disc ML-DmD9		273
wing disc ML-DmD16-c3		160
wing disc ML-DmD21		128
wing disc ML-DmD32		163
haltere disc ML-DmD17-c3		207
eye-antennal disc ML-DmD11		214
antennal disc ML-DmD20-c5		149
mixed discs ML-DmD4-c1		171
CNS ML-DmBG1-c1		160
CNS ML-DmBG2-c2		156
tumorous blood cells mbn2		152
ovary fGS/OSS		156
ovary OSC		288
ovary OSS		150
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		177
Schneider line 2 Sg4		101
embryonic 1182-4H		180
embryonic GM2		112
embryonic Kc167		145
embryonic S1		257
embryonic S3		183
leg disc CME L1		193
wing disc CME-W2		143
wing disc ML-DmD8		201
wing disc ML-DmD9		273
wing disc ML-DmD16-c3		160
wing disc ML-DmD21		128
wing disc ML-DmD32		163
haltere disc ML-DmD17-c3		207
eye-antennal disc ML-DmD11		214
antennal disc ML-DmD20-c5		149
mixed discs ML-DmD4-c1		171
CNS ML-DmBG1-c1		160
CNS ML-DmBG2-c2		156
tumorous blood cells mbn2		152
ovary fGS/OSS		156
ovary OSC		288
ovary OSS		150
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(177)
Schneider line 2 Sg4		(101)
embryonic 1182-4H		(180)
embryonic GM2		(112)
embryonic Kc167		(145)
embryonic S1		(257)
embryonic S3		(183)
leg disc CME L1		(193)
wing disc CME-W2		(143)
wing disc ML-DmD8		(201)
wing disc ML-DmD9		(273)
wing disc ML-DmD16-c3		(160)
wing disc ML-DmD21		(128)
wing disc ML-DmD32		(163)
haltere disc ML-DmD17-c3		(207)
eye-antennal disc ML-DmD11		(214)
antennal disc ML-DmD20-c5		(149)
mixed discs ML-DmD4-c1		(171)
CNS ML-DmBG1-c1		(160)
CNS ML-DmBG2-c2		(156)
tumorous blood cells mbn2		(152)
ovary fGS/OSS		(156)
ovary OSC		(288)
ovary OSS		(150)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(177)
Schneider line 2 Sg4		101
embryonic 1182-4H		(180)
embryonic GM2		112
embryonic Kc167		(145)
embryonic S1		(257)
embryonic S3		(183)
leg disc CME L1		(193)
wing disc CME-W2		143
wing disc ML-DmD8		(201)
wing disc ML-DmD9		(273)
wing disc ML-DmD16-c3		(160)
wing disc ML-DmD21		128
wing disc ML-DmD32		(163)
haltere disc ML-DmD17-c3		(207)
eye-antennal disc ML-DmD11		(214)
antennal disc ML-DmD20-c5		(149)
mixed discs ML-DmD4-c1		(171)
CNS ML-DmBG1-c1		(160)
CNS ML-DmBG2-c2		(156)
tumorous blood cells mbn2		(152)
ovary fGS/OSS		(156)
ovary OSC		(288)
ovary OSS		(150)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		177
Schneider line 2 Sg4		101
embryonic 1182-4H		180
embryonic GM2		112
embryonic Kc167		145
embryonic S1		257
embryonic S3		183
leg disc CME L1		193
wing disc CME-W2		143
wing disc ML-DmD8		201
wing disc ML-DmD9		273
wing disc ML-DmD16-c3		160
wing disc ML-DmD21		128
wing disc ML-DmD32		163
haltere disc ML-DmD17-c3		207
eye-antennal disc ML-DmD11		214
antennal disc ML-DmD20-c5		149
mixed discs ML-DmD4-c1		171
CNS ML-DmBG1-c1		160
CNS ML-DmBG2-c2		156
tumorous blood cells mbn2		152
ovary fGS/OSS		156
ovary OSC		288
ovary OSS		150
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		173
cold shock, 4-day adult		122
heat shock, 4-day adult		211
Cadmium 50 mM 6 hrs, larvae L3		127
Cadmium 50 mM 12 hrs, larvae L3		174
Cadmium 50 mM 48 hrs, 4-day adult		236
Cadmium 100 mM 48 hrs, 4-day adult		372
Copper 0.5 mM 12 hrs, larvae L3		104
Copper 15 mM 48 hrs, 4-day adult		191
Zinc 5 mM 12 hrs, larvae L3		106
Zinc 4.5 mM 48 hrs, 4-day adult		135
Ethanol 2.5% 3 hrs, larvae L3		353
Ethanol 5% 3 hrs, larvae L3		119
Ethanol 10% 3 hrs, larvae L3		120
Caffeine 1.5 mg/ml 4 hrs, larvae L3		106
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		401
Caffeine 25 mg/ml 48 hrs, 4-day adult		171
Paraquat 5 mM 48 hrs, 4-day adult		329
Paraquat 10 mM 48 hrs, 4-day adult		226
Rotenone 2 μg 12 hrs, larvae L3		144
Rotenone 8 μg 12 hrs, larvae L3		141
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(173)
cold shock, 4-day adult		(122)
heat shock, 4-day adult		(211)
Cadmium 50 mM 6 hrs, larvae L3		(127)
Cadmium 50 mM 12 hrs, larvae L3		(174)
Cadmium 50 mM 48 hrs, 4-day adult		(236)
Cadmium 100 mM 48 hrs, 4-day adult		(372)
Copper 0.5 mM 12 hrs, larvae L3		(104)
Copper 15 mM 48 hrs, 4-day adult		(191)
Zinc 5 mM 12 hrs, larvae L3		(106)
Zinc 4.5 mM 48 hrs, 4-day adult		(135)
Ethanol 2.5% 3 hrs, larvae L3		(353)
Ethanol 5% 3 hrs, larvae L3		(119)
Ethanol 10% 3 hrs, larvae L3		(120)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(106)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(401)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(171)
Paraquat 5 mM 48 hrs, 4-day adult		(329)
Paraquat 10 mM 48 hrs, 4-day adult		(226)
Rotenone 2 μg 12 hrs, larvae L3		(144)
Rotenone 8 μg 12 hrs, larvae L3		(141)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		(173)
cold shock, 4-day adult		(122)
heat shock, 4-day adult		(211)
Cadmium 50 mM 6 hrs, larvae L3		(127)
Cadmium 50 mM 12 hrs, larvae L3		(174)
Cadmium 50 mM 48 hrs, 4-day adult		(236)
Cadmium 100 mM 48 hrs, 4-day adult		(372)
Copper 0.5 mM 12 hrs, larvae L3		104
Copper 15 mM 48 hrs, 4-day adult		(191)
Zinc 5 mM 12 hrs, larvae L3		106
Zinc 4.5 mM 48 hrs, 4-day adult		(135)
Ethanol 2.5% 3 hrs, larvae L3		(353)
Ethanol 5% 3 hrs, larvae L3		(119)
Ethanol 10% 3 hrs, larvae L3		(120)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		106
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(401)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(171)
Paraquat 5 mM 48 hrs, 4-day adult		(329)
Paraquat 10 mM 48 hrs, 4-day adult		(226)
Rotenone 2 μg 12 hrs, larvae L3		(144)
Rotenone 8 μg 12 hrs, larvae L3		(141)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		173
cold shock, 4-day adult		122
heat shock, 4-day adult		211
Cadmium 50 mM 6 hrs, larvae L3		127
Cadmium 50 mM 12 hrs, larvae L3		174
Cadmium 50 mM 48 hrs, 4-day adult		236
Cadmium 100 mM 48 hrs, 4-day adult		372
Copper 0.5 mM 12 hrs, larvae L3		104
Copper 15 mM 48 hrs, 4-day adult		191
Zinc 5 mM 12 hrs, larvae L3		106
Zinc 4.5 mM 48 hrs, 4-day adult		135
Ethanol 2.5% 3 hrs, larvae L3		353
Ethanol 5% 3 hrs, larvae L3		119
Ethanol 10% 3 hrs, larvae L3		120
Caffeine 1.5 mg/ml 4 hrs, larvae L3		106
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		401
Caffeine 25 mg/ml 48 hrs, 4-day adult		171
Paraquat 5 mM 48 hrs, 4-day adult		329
Paraquat 10 mM 48 hrs, 4-day adult		226
Rotenone 2 μg 12 hrs, larvae L3		144
Rotenone 8 μg 12 hrs, larvae L3		141
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		173
cold shock, 4-day adult		122
heat shock, 4-day adult		211
Cadmium 50 mM 6 hrs, larvae L3		127
Cadmium 50 mM 12 hrs, larvae L3		174
Cadmium 50 mM 48 hrs, 4-day adult		236
Cadmium 100 mM 48 hrs, 4-day adult		372
Copper 0.5 mM 12 hrs, larvae L3		104
Copper 15 mM 48 hrs, 4-day adult		191
Zinc 5 mM 12 hrs, larvae L3		106
Zinc 4.5 mM 48 hrs, 4-day adult		135
Ethanol 2.5% 3 hrs, larvae L3		353
Ethanol 5% 3 hrs, larvae L3		119
Ethanol 10% 3 hrs, larvae L3		120
Caffeine 1.5 mg/ml 4 hrs, larvae L3		106
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		401
Caffeine 25 mg/ml 48 hrs, 4-day adult		171
Paraquat 5 mM 48 hrs, 4-day adult		329
Paraquat 10 mM 48 hrs, 4-day adult		226
Rotenone 2 μg 12 hrs, larvae L3		144
Rotenone 8 μg 12 hrs, larvae L3		141
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(173)
cold shock, 4-day adult		(122)
heat shock, 4-day adult		(211)
Cadmium 50 mM 6 hrs, larvae L3		(127)
Cadmium 50 mM 12 hrs, larvae L3		(174)
Cadmium 50 mM 48 hrs, 4-day adult		(236)
Cadmium 100 mM 48 hrs, 4-day adult		(372)
Copper 0.5 mM 12 hrs, larvae L3		(104)
Copper 15 mM 48 hrs, 4-day adult		(191)
Zinc 5 mM 12 hrs, larvae L3		(106)
Zinc 4.5 mM 48 hrs, 4-day adult		(135)
Ethanol 2.5% 3 hrs, larvae L3		(353)
Ethanol 5% 3 hrs, larvae L3		(119)
Ethanol 10% 3 hrs, larvae L3		(120)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(106)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(401)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(171)
Paraquat 5 mM 48 hrs, 4-day adult		(329)
Paraquat 10 mM 48 hrs, 4-day adult		(226)
Rotenone 2 μg 12 hrs, larvae L3		(144)
Rotenone 8 μg 12 hrs, larvae L3		(141)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		(173)
cold shock, 4-day adult		122
heat shock, 4-day adult		(211)
Cadmium 50 mM 6 hrs, larvae L3		127
Cadmium 50 mM 12 hrs, larvae L3		(174)
Cadmium 50 mM 48 hrs, 4-day adult		(236)
Cadmium 100 mM 48 hrs, 4-day adult		(372)
Copper 0.5 mM 12 hrs, larvae L3		104
Copper 15 mM 48 hrs, 4-day adult		(191)
Zinc 5 mM 12 hrs, larvae L3		106
Zinc 4.5 mM 48 hrs, 4-day adult		135
Ethanol 2.5% 3 hrs, larvae L3		(353)
Ethanol 5% 3 hrs, larvae L3		119
Ethanol 10% 3 hrs, larvae L3		120
Caffeine 1.5 mg/ml 4 hrs, larvae L3		106
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(401)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(171)
Paraquat 5 mM 48 hrs, 4-day adult		(329)
Paraquat 10 mM 48 hrs, 4-day adult		(226)
Rotenone 2 μg 12 hrs, larvae L3		(144)
Rotenone 8 μg 12 hrs, larvae L3		141
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		173
cold shock, 4-day adult		122
heat shock, 4-day adult		211
Cadmium 50 mM 6 hrs, larvae L3		127
Cadmium 50 mM 12 hrs, larvae L3		174
Cadmium 50 mM 48 hrs, 4-day adult		236
Cadmium 100 mM 48 hrs, 4-day adult		372
Copper 0.5 mM 12 hrs, larvae L3		104
Copper 15 mM 48 hrs, 4-day adult		191
Zinc 5 mM 12 hrs, larvae L3		106
Zinc 4.5 mM 48 hrs, 4-day adult		135
Ethanol 2.5% 3 hrs, larvae L3		353
Ethanol 5% 3 hrs, larvae L3		119
Ethanol 10% 3 hrs, larvae L3		120
Caffeine 1.5 mg/ml 4 hrs, larvae L3		106
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		401
Caffeine 25 mg/ml 48 hrs, 4-day adult		171
Paraquat 5 mM 48 hrs, 4-day adult		329
Paraquat 10 mM 48 hrs, 4-day adult		226
Rotenone 2 μg 12 hrs, larvae L3		144
Rotenone 8 μg 12 hrs, larvae L3		141
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Show all FlyExpress processed images for eIF-4E

BDGP expression data

•

RE36735

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015218

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4035-RB

FlyExpress - Embryonic expression images (BDGP data)

•

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal

lethal, with Scer\GAL4^Act5C.PU

eIF-4E^GD1432

lethal, with Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH

eIF-4E^GD1432

lethal, with Scer\GAL4^pnr-MD237

eIF-4E^GD1432

lethal (with Df(3L)29A6)

lethal (with Df(3L)AC1)

eIF-4E^S099905

lethal | blastoderm stage, with Scer\GAL4^en-e16E

eIF-4E^1.Scer\UAS

lethal | embryonic stage | recessive

lethal | first instar larval stage

eIF-4E^67Af

lethal | first instar larval stage (with eIF-4E^67Af)

eIF-4E^Scim-a

lethal | first instar larval stage (with eIF-4E^Scim-a)

eIF-4E^67Af

lethal | larval stage | recessive

lethal | male, with Scer\GAL4^Bx-MS1096

eIF-4E^GD1432

lethal | pupal stage, with Scer\GAL4^pnr-MD237

eIF-4E^GD1432

lethal | recessive

lethal | third instar larval stage

eIF-4E^Scim-a

partially lethal - majority die | female, with Scer\GAL4^Bx-MS1096

eIF-4E^GD1432

viable

eIF-4E^CC00392

viable | reduced, with eIF-4E^67Af

eIF-4E^{Ser251Ala.t8.9}

viable | reduced, with eIF-4E⁰⁷²³⁸

eIF-4E^{Ser251Ala.t8.9}

viable | reduced, with eIF-4E^S058911

eIF-4E^{Ser251Ala.t8.9}

viable | reduced, with eIF-4E^S071513

eIF-4E^{Ser251Ala.t8.9}

viable | reduced, with eIF-4E^{Ser251Ala.t8.9}

Sterility

Allele

female sterile | germline clone

eIF-4E^67Af

Other Phenotypes

Allele

chemical sensitive, with Scer\GAL4^da.G32

eIF-4E^Scer\UAS.cRa

decreased cell size, with eIF-4E^S058911

eIF-4E^{Ser251Ala.t8.9}

decreased cell size, with eIF-4E^{Ser251Ala.t8.9}

eIF-4E^S058911

developmental rate defective

developmental rate defective, with eIF-4E^67Af

eIF-4E^{Ser251Ala.t8.9}

developmental rate defective, with eIF-4E⁰⁷²³⁸

eIF-4E^{Ser251Ala.t8.9}

developmental rate defective, with eIF-4E^S058911

eIF-4E^{Ser251Ala.t8.9}

developmental rate defective, with eIF-4E^S071513

eIF-4E^{Ser251Ala.t8.9}

developmental rate defective, with eIF-4E^{Ser251Ala.t8.9}

increased cell death | embryonic stage

eIF-4E^67Af

increased cell size, with eIF-4E^S058911

eIF-4E^{Ser251Asp.t8.9}

increased cell size, with eIF-4E^{Ser251Asp.t8.9}

eIF-4E^S058911

meiotic cell cycle defective | dominant

mitotic cell cycle defective

neuroanatomy defective, with Scer\GAL4^Mhc.PW

eIF-4E^Scer\UAS.cRa

neuroanatomy defective, with Scer\GAL4^{Mhc.Switch.PO}

eIF-4E^Scer\UAS.cRa

neuroanatomy defective | adult stage, with Scer\GAL4^ple.PF

eIF-4E^Scer\UAS.cRa

neuroanatomy defective | larval stage

eIF-4E^GD1432

neurophysiology defective, with Scer\GAL4^Mhc.PW

eIF-4E^Scer\UAS.cRa

visible, with eIF-4E^67Af

eIF-4E^{Ser251Ala.t8.9}

visible, with eIF-4E⁰⁷²³⁸

eIF-4E^{Ser251Ala.t8.9}

visible, with eIF-4E^S058911

eIF-4E^{Ser251Ala.t8.9}

visible, with eIF-4E^S071513

eIF-4E^{Ser251Ala.t8.9}

visible, with eIF-4E^{Ser251Ala.t8.9}

wild-type

eIF-4E^+t8.9

Phenotype manifest in

Allele

bouton, with Scer\GAL4^Mhc.PW

eIF-4E^Scer\UAS.cRa

dopaminergic neuron, with Scer\GAL4^ple.PF

eIF-4E^Scer\UAS.cRa

embryonic/first instar larval cuticle, with Scer\GAL4^nos.PG

eIF-4E^1.Scer\UAS

embryonic/larval neuroblast | larval stage

eIF-4E^GD1432

embryonic/larval neuromuscular junction, with Scer\GAL4^{Mhc.Switch.PO}

eIF-4E^Scer\UAS.cRa

eye, with Scer\GAL4^GMR.PF

eIF-4E^1.Scer\UAS

eye, with Scer\GAL4^hs.2sev

eIF-4E^1.Scer\UAS

ganglion mother cell | larval stage

eIF-4E^GD1432

interommatidial bristle | ectopic, with Scer\GAL4^ey.PH

eIF-4E^1.Scer\UAS

interommatidial bristle | ectopic, with Scer\GAL4^GMR.PF

eIF-4E^1.Scer\UAS

interommatidial bristle | ectopic, with Scer\GAL4^hs.2sev

eIF-4E^1.Scer\UAS

interphase of mitotic cell cycle

intersegmental nerve, with Scer\GAL4^elav-C155

eIF-4E^EP568

mitosis & nuclear chromosome

neuromuscular junction, with Scer\GAL4^Mhc.PW

eIF-4E^Scer\UAS.cRa

ommatidium, with eIF-4E^S058911

eIF-4E^{Ser251Ala.t8.9}

ommatidium, with eIF-4E^{Ser251Ala.t8.9}

eIF-4E^S058911

type I bouton, with Scer\GAL4^{Mhc.Switch.PO}

eIF-4E^Scer\UAS.cRa

ventral furrow

eIF-4E^dsRNA.cGa

wing, with eIF-4E^67Af

eIF-4E^{Ser251Ala.t8.9}

wing, with eIF-4E⁰⁷²³⁸

eIF-4E^{Ser251Ala.t8.9}

wing, with eIF-4E^S058911

eIF-4E^{Ser251Ala.t8.9}

wing, with eIF-4E^S071513

eIF-4E^{Ser251Ala.t8.9}

wing, with eIF-4E^{Ser251Ala.t8.9}

wing, with Scer\GAL4^Bx-MS1096

eIF-4E^GD1432

wing disc, with eIF-4E^S058911

wing disc, with eIF-4E^{Ser251Ala.t8.9}

eIF-4E^S058911

wing disc, with eIF-4E^{Ser251Asp.t8.9}

eIF-4E^S058911

Classical Alleles ( 26 )

For All Classical Alleles Show

Allele of eIF-4E	Class	Mutagen	Stocks	Known lesion
eIF-4E^S058911		Delta2-3	3	Yes
eIF-4E^67Af	loss of function allele	ethyl methanesulfonate	2	Yes
eIF-4E⁰⁷²³⁸		P-element activity	1	Yes
eIF-4E^5-HA-1527			1	--
eIF-4E^5-HA-2814			1	--
eIF-4E^5-SZ-3171			1	--
eIF-4E^d03437		P-element activity	1	--
eIF-4E^e03130		piggyBac transposase	1	--
eIF-4E^f03004		piggyBac transposase	1	--
eIF-4E^f05739		piggyBac transposase	1	--
eIF-4E²¹⁸⁸		ethyl methanesulfonate	0	--
eIF-4E^CC00392		P-element activity	0	Yes
eIF-4E^d11121		P-element activity	0	--
eIF-4E^DOr		P-element activity	0	--
eIF-4E^EP3371		P-element activity	0	--
eIF-4E^EP568		P-element activity	0	Yes
eIF-4E^GS-A2783		Delta2-3	0	--
eIF-4E^S025007		Delta2-3 P-element activity	0	--
eIF-4E^S026009		Delta2-3 P-element activity	0	--
eIF-4E^S058711		Delta2-3 P-element activity	0	--
eIF-4E^S071513			0	Yes
eIF-4E^S091912		Delta2-3 P-element activity	0	--
eIF-4E^S099905		Delta2-3 P-element activity	0	--
eIF-4E^S100413		Delta2-3 P-element activity	0	--
eIF-4E^Scim-a	hypomorphic allele - genetic evidence	Delta2-3	0	Yes
eIF-4E^Scim-b		Delta2-3	0	Yes

Alleles Carried on Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Allele of eIF-4E	Mutagen	Stocks	Known lesion
eIF-4E^GD1432	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
eIF-4E^HMS00969	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
eIF-4E^KK108104	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
eIF-4E^{S251A.Scer\UAS}	in vitro construct - amino acid replacement in vitro construct - regulatory fusion	1	Yes
eIF-4E^Scer\UAS.cAa	in vitro construct - regulatory fusion	1	Yes
eIF-4E^Scer\UAS.cRa	in vitro construct - regulatory fusion	1	Yes
eIF-4E^{Ser251Ala.t8.9}	in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis	1	Yes
eIF-4E^{Ser251Asp.t8.9}	in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis	1	Yes
eIF-4E^+t8.9	in vitro construct - genomic fragment	0	Yes
eIF-4E^1.Scer\UAS	in vitro construct - regulatory fusion	0	Yes
eIF-4E^dsRNA.cGa	in vitro construct - RNAi	0	Yes
eIF-4E^NIG.4035R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
eIF-4E^{S251A.T:Hsap\MYC}	in vitro construct - coding region fusion in vitro construct - amino acid replacement	0	Yes
eIF-4E^S251D	in vitro construct - amino acid replacement	0	Yes
eIF-4E^T:Hsap\MYC	in vitro construct - coding region fusion	0	Yes
eIF-4E^VDRC.cUa	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(3L)29A6

(Szeged Stock Center, 1998-, Kankel et al., 2004, Hernandez et al., 2005, Hernandez et al., 1997, Deak et al., 1997)

Df(3L)AC1

(Szeged Stock Center, 1998-, Spradling et al., 1999, BDGP Project Members, 1994-1999, Hernandez et al., 1997)

Df(3L)BSC113

(Andrade and Cook, 2005.7.22)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

characterization construct

P{eIF-4E^S251D}

P{eIF-4E^Ser251Ala.8.9}

P{eIF-4E^Ser251Asp.8.9}

P{eIF-4E^wt.8.9}

P{VDRC.eIF-4E.U}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}eIF-4E^EP568

P{EP}eIF-4E^EP3371

P{EP}EP3583-13

P{GSV1}eIF-4E^GS-A2783

P{XP}eIF-4E^d03437

P{XP}eIF-4E^d11121

insertion of enhancer trap

P{lacW}eIF-4E^S025007

P{lacW}eIF-4E^S026009

P{lacW}eIF-4E^S058711

P{lacW}eIF-4E^S058911

P{lacW}eIF-4E^S071513

P{lacW}eIF-4E^S091912

P{lacW}eIF-4E^S099905

P{lacW}eIF-4E^S100413

P{lacW}l(3)SH066^SH066

P{PZ}eIF-4E⁰⁷²³⁸

miscellaneous insertions

P{(loxP.w.-110+276)w}eIF-4E^DOr

P{PTT-GC}CC00375

P{PTT-GC}CC01442

P{PTT-GC}CC01492

P{PTT-GC}eIF-4E^CC00392

P{RS5}eIF-4E^5-HA-1527

P{RS5}eIF-4E^5-HA-2814

P{RS5}eIF-4E^5-SZ-3171

P{SUPor-P}eIF-4E^Scim-a

P{SUPor-P}eIF-4E^Scim-b

PBac{RB}eIF-4E^e03130

PBac{WH}eIF-4E^f03004

PBac{WH}eIF-4E^f05739

Gene Ontology: Function, Process & Cellular Component ( 26 unique terms )

Terms Based on Experimental Evidence ( 24 terms )

Molecular Function

CV term

eukaryotic initiation factor 4G binding

References

inferred from physical interaction with eIF4G

(Hernandez et al., 2005, Hernandez et al., 1998)

protein binding

inferred from physical interaction with Thor

RNA 7-methylguanosine cap binding

inferred from direct assay

(Hernandez et al., 2005, Zapata et al., 1994, Cho et al., 2005, Hernandez et al., 2012)

RNA cap binding

inferred from direct assay

(assigned by UniProtKB)

translation initiation factor activity

inferred from direct assay

(assigned by UniProtKB)

inferred from genetic interaction with Saccharomyces CDC33

Biological Process

CV term

References

autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

chromatin organization

inferred from mutant phenotype

(Dobie et al., 2001)

embryo development

inferred from mutant phenotype

female meiosis chromosome segregation

inferred from mutant phenotype

(Dobie et al., 2001)

mitosis

inferred from mutant phenotype

(Dobie et al., 2001, Ducat et al., 2008)

mitotic chromosome condensation

inferred from mutant phenotype

(Dobie et al., 2001)

mitotic spindle elongation

inferred from mutant phenotype

(Goshima et al., 2007)

mitotic spindle organization

inferred from mutant phenotype

(Goshima et al., 2007)

neurogenesis

inferred from mutant phenotype

(Neumüller et al., 2011)

regulation of cell growth

inferred from mutant phenotype

RNA metabolic process

inferred from direct assay

(assigned by UniProtKB)

salivary gland cell autophagic cell death

inferred from expression pattern

(Gorski et al., 2003)

translation

inferred from direct assay

translational initiation

inferred from direct assay

(assigned by UniProtKB)

inferred from genetic interaction with Saccharomyces CDC33

Cellular Component

CV term

(Sigrist et al., 2000, Ottone et al., 2011)

References

cytoplasm

inferred from direct assay

cytoplasmic mRNA processing body

inferred from direct assay

(Liu and Gall, 2007)

eukaryotic translation initiation factor 4F complex

inferred from direct assay

inferred from physical interaction with eIF4G

microtubule associated complex

inferred from direct assay

(Hughes et al., 2008)

postsynaptic density

inferred from direct assay

(Menon et al., 2004)

Terms Based on Predictions or Assertions ( 8 terms )

Molecular Function

CV term

References

RNA cap binding

inferred from sequence or structural similarity

non-traceable author statement

translation initiation factor activity

inferred from sequence or structural similarity

inferred from sequence or structural similarity with human EIF4E AND inferred from sequence or structural similarity with mouse Eif4e

(Lasko, 1996.4.9, Sierra, 1994.10.19, Swiss-Prot Project Members, 1996.2.1)

non-traceable author statement

Biological Process

CV term

positive regulation of cell growth

References

traceable author statement

(Oldham and Hafen, 2003)

RNA metabolic process

non-traceable author statement

translation

inferred from sequence or structural similarity with human EIF4E AND inferred from sequence or structural similarity with mouse Eif4e

non-traceable author statement

(Lasko, 1996.4.9, Sierra, 1994.10.19)

translational initiation

non-traceable author statement

Cellular Component

CV term

References

cytosol

inferred from sequence or structural similarity

inferred from sequence or structural similarity with human EIF4E AND inferred from sequence or structural similarity with mouse Eif4e

eukaryotic translation initiation factor 4F complex

non-traceable author statement

(Lasko, 1996.4.9, Sierra, 1994.10.19)

inferred from sequence or structural similarity

non-traceable author statement

non-traceable author statement

(assigned by UniProtKB)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

eIF-4E - AP-2σ

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Aats-lys

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG10306

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG11107

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG15220

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG1701

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG2199

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG2950

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG3689

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG5746

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG6701

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - CG8545

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Cdc5

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Gie

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Gnf1

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Ku80

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Ote

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - RPA2

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - RpL35A

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - RpLP0

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - RpS12

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Rpt2

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Rrp46

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Rs1

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Sec23

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - SmG

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - TFAM

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Tace

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - Thor

two hybrid, anti bait coimmunoprecipitation, western blot

eIF-4E - bocksbeutel

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - cup

two hybrid

(Wilhelm et al., 2003)

eIF-4E - eIF4E-5

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - eIF4G

anti bait coimmunoprecipitation, western blot

eIF-4E - eIF4G2

anti tag coimmunoprecipitation, western blot, two hybrid

eIF-4E - ens

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - l(1)G0230

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - l(2)37Cb

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - mRpL20

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - mRpL52

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - mRpS24

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - mRpS28

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - mus210

anti tag coimmunoprecipitation, peptide massfingerprinting

eIF-4E - park

pull down, western blot

(Ottone et al., 2011)

eIF-4E - tyf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Zappavigna et al., 2004)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

eIF-4E allele

Gene

References

eIF-4E^67Af

Lk6

(Arquier et al., 2005)

eIF-4E²¹⁸⁸

ftz

(Kankel et al., 2004)

eIF-4E⁰⁷²³⁸

cup

eIF-4E^S251D

aret

(Yan and Macdonald, 2004)

eIF-4E^Scim-a

Lk6

(Arquier et al., 2005)

unspecified

(Krupp et al., 2005)

Lk6

(Reiling et al., 2005)

External Data

BioGRID - A database of protein and genetic interactions

•

69740

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0015218

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0015218

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

45525

Orthologs

OrthoDB Orthologs (51) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6DV66M

Dipteran inclusive ortholog search

EOG683DDP

Insect inclusive ortholog search

EOG663Z86

Arthropod inclusive ortholog search

EOG6MPG63

Metazoa inclusive ortholog search

EOG6QZMF3

Orthologs in Drosophila Species (EOG6DV66M)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA28658

Drosophila persimilis

Dper\GL12850

Drosophila willistoni

Dwil\GK25580

Drosophila willistoni

Dwil\GK14373

Drosophila willistoni

Dwil\GK12168

Drosophila willistoni

Dwil\GK20927

Drosophila virilis

Dvir\GJ19475

Drosophila grimshawi

Dgri\GH24331

Orthologs in non-Drosophila Dipterans (EOG683DDP)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001916

Anopheles gambiae

Malaria mosquito

Agam\AGAP007172

Orthologs in non-Dipteran Insects (EOG663Z86)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB55977

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH26160

Atta cephalotes

Leafcutter ant

Acep\ACEP14824

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO23370

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL20787

Linepithema humile

Argentine ant

Lhum\LH22341

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB22892

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009961

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI084396

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001956

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001300

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI30940

Bombyx mori

Silkmoth

Bmor\BGIBMGA012674

Bombyx mori

Silkmoth

Bmor\Bmo.6024

Tribolium castaneum

Red flour beetle

Tcas\eIF-4E

Orthologs in non-Insect Arthropods (EOG6MPG63)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_98425

Ixodes scapularis

Deer tick

Isca\ISCW021213

Orthologs in non-Arthropod Metazoa (EOG6QZMF3)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\ife-1

Caenorhabditis elegans

Nematode

Cele\ife-2

Caenorhabditis elegans

Nematode

Cele\B0348.6

Caenorhabditis elegans

Nematode

Cele\ife-5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\eIF4E1

Danio rerio

Zebrafish

Drer\CH211-200O8.5

Danio rerio

Zebrafish

Drer\DKEY-228D14.3

Danio rerio

Zebrafish

Drer\eif4e1b

Danio rerio

Zebrafish

Drer\eif4ea

Xenopus tropicalis

Western clawed frog

Xtro\eif4e

Xenopus tropicalis

Western clawed frog

Xtro\eif4e1b

Gallus gallus

Domestic chicken

Ggal\EIF4E

Mus musculus

House mouse

Mmus\ENSMUSG00000091743

Mus musculus

House mouse

Mmus\Eif4e

Mus musculus

House mouse

Mmus\Eif4e1b

Rattus norvegicus

Norway rat

Rnor\Eif4e1b

Rattus norvegicus

Norway rat

Rnor\Eif4e

Homo sapiens

Human

Hsap\EIF4E

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\EIF4E

AAA Orthologs (7) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA28658

Drosophila persimilis

Dper\GL12850

Stocks & Reagents

Stocks Listed in FlyBase ( 19 )

Bloomington

8650

w^*; P{UAS-eIF-4E.R}2

8710

w¹¹¹⁸; P{UAS-eIF-4E.A}26

8711

w¹¹¹⁸; P{UAS-eIF-4E.S251A}8

Harvard

P{XP}eIF-4E^d03437

PBac{RB}eIF-4E^e03130

PBac{WH}eIF-4E^f03004

PBac{WH}eIF-4E^f05739

Kyoto

125237

w¹¹¹⁸; P{RS5}eIF-4E^5-HA-1527

125689

w¹¹¹⁸; P{RS5}eIF-4E^5-HA-2814

125931

w¹¹¹⁸; P{RS5}eIF-4E^5-SZ-3171

VDRC

v100722

P{KK108104}VIE-260B

v7800

w¹¹¹⁸; P{GD1432}v7800

More stocks available...

Genomic Clones ( 1 )

BACR01E20

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 158 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

(Maroto and Sierra, 1989, Igreja and Izaurralde, 2011)

DRSC - Results from RNAi screens.

•

FBgn0015218

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

45525

Antibody Information

polyclonal

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Source for merge of: eIF-4E l(3)67Af

(Szeged Stock Center, 1998-)

Source for merge of: eIF-4E l(3)07238

(Spradling et al., 1999)

Source for merge of: eIF-4E l(3)S025007 l(3)S026009 l(3)S058711 l(3)S091912

Additional comments

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

(Goshima et al., 2007)

dsRNA made from templates generated with primers directed against this gene reduces mean cell diameter. Silencing both eIF-4E and S6k together does not reduce mean cell diameter to the same extent as rapamycin treatment.

(Guertin et al., 2006)

cup is required to localise eIF-4E to the posterior of the oocyte.

(Wilhelm et al., 2003)

eIF-4E is phosphorylated on Ser251.

The phosphorylation of eIF-4E at Ser251 is biologically significant and is essential for normal growth and development.

Identification: Enhancer trap expression pattern survey for loci expressed in the ring gland.

(Harvie et al., 1998)

Cloning and sequencing of eIF-4E revealed the existance of three mRNAs that are generated by alternative splicing of a primary transcript and all have different 5' untranslated leader regions. eIF-4E is a single copy gene. Expression of eIF-4E is spatially and temporally controlled during embryonic development. eIF-4E is ubiquitously expressed during embryogenesis but transcripts preferentially accumulate in certain tissues, particularly in the pole cells at different developmental stages.

eIF-4E produces two different cap-binding proteins by alternative splicing.

eIF-4E protein from both control and heat shocked cells has been purified and characterised.

(Duncan et al., 1995)

Isolated from a 0 to 20 hour embryo cDNA library using a polyclonal antibody against eIF-4E.

An eIF-4E cDNA has been cloned and sequenced, and its expression pattern has been analysed.

Drosophila elongation factor 4F consists of two subunits, a polypeptide of about 200kD and the product of eIF-4E, the cap-binding protein.

(Hernandez et al., 1995)

Drosophila initiation factor eIF-4F is composed of two subunits, one is encoded by eIF-4E and the other by eIF-4G, a 200kD protein.

(Gong et al., 2004, Kraut et al., 2001, Kraut et al., 2001, Lasko, 2000, Quinones-Coello, 2007, Goshima et al., 2007, Slack et al., 2010, Ducat et al., 2008, Pisa et al., 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Jäger and Dorner, 2010, Ni et al., 2010.12.1, Cammarato et al., 2011, Puri et al., 2008, Tettweiler et al., 2012)

Translation of the majority of non-heat shock mRNAs and Hsp83 mRNA is very dependent on eIF-4E. In contrast, translation of Hsp70 mRNAs and to a lesser extent the mRNAs for the small heat shock proteins is almost independent of eIF-4E.

(Zapata et al., 1991)

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

Entrez Gene - A searchable database of RefSeq genes.

•

45525

UniProtKB/Swiss-Prot

•

P48598

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Translation Initiation factor eIF- 4e (IPR001040)

Eukaryotic translation initiation factor 4E (eIF-4E), conserved site (IPR019770)

Translation Initiation factor eIF- 4e-like domain (IPR023398)

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

•

RE36735

Linkouts

BioGRID - A database of protein and genetic interactions

•

69740

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0015218

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0015218

DRSC - Results from RNAi screens.

•

FBgn0015218

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015218

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4035-RB

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0015218

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

45525

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

45525

modMine - Data generated by the modENCODE project.

•

FBgn0015218

Synonyms & Secondary IDs ( 49 )

Reported As

Symbol Synonym

0260/09

0587/11

0589/11

0919/12

1004/13

7238

(Harvie et al., 1998)

CBP

(Whitman, 2005, Zapata et al., 1991, Jacquier et al., 2007)

CG4035

CT13384

(Xie and Ding, 2000)

CT39424

(Xie and Ding, 2000)

CT39426

(Xie and Ding, 2000)

deIF4E

(Miron et al., 2001, Imai et al., 2008, Read et al., 2009, Arquier et al., 2008, Garrey et al., )

D-eIF4E

(Myrick and Dearolf, 2000)

deIF-4E

(Guertin et al., 2006)

dEif4e

(Wessells et al., 2009)

eIF4E

(McNeill et al., 2008, Teleman et al., 2005, Macdonald, 2005, Semotok et al., 2005, Reiling et al., 2005, Zappavigna et al., 2004, Wilhelm et al., 2003, Sigrist et al., 2002, Yan and Macdonald, 2004, Lachance et al., 2002, Macdonald, 2004, Wilhelm et al., 2004, Jenkins and Lasko, 2004, Graham et al., 2004, Saucedo and Edgar, 2002, Chakkalakal and Jasmin, 2003, Oldham and Hafen, 2003, Darnell, 2002, Wilhelm et al., 2003, Gamberi et al., 2003, Graham et al., 2003, Niessing et al., 2002, Van Buskirk and Schupbach, 2002, Graham et al., 2001, Lasko, 2000, DiAntonio, 2000, Sigrist et al., 2000, Bernal and Kimbrell, 2000, Graham et al., 2000, Schoenfeld et al., 2000, Miron et al., 2000, Carrera et al., 2000, Lehner, 1999, Lasko, 1996.4.9, Miron et al., 1999, Lachance et al., 1999, Lachance et al., 1998, Miron et al., 1997, Lachance and Lasko, 1997, Lavoie et al., 1996, Bourouis et al., 2005, Baker and Fuller, 2006, Cho et al., 2005, Kozak, 2006, Lasko and Sonenberg, 2007, Lee et al., 2007, Hernandez et al., 2008, Franklin-Dumont et al., 2007, Layalle et al., 2008, Grewal et al., 2007, Hughes et al., 2008, Li and Li, 2006, Imai et al., 2008, Kwak et al., 2008, Parra-Palau et al., 2005, Read et al., 2009, Iwasaki et al., 2009, Nuzhdin et al., 2009, Mihailovich et al., 2012, Nie et al., 2009, Lee et al., 2009, Teleman et al., 2008, Shen et al., 2009, Duncan et al., 2009, Liu and Gall, 2007, Farny et al., 2009, Cauchi et al., 2010, Jäger and Dorner, 2010, Ottone et al., 2011, Garrey et al., , Gehrke et al., 2010, Hernandez et al., 2004, Singh et al., 2011, Lim et al., 2011, Duncan, 2008, Ganesan et al., 2011, Yarunin et al., 2011, Lee et al., 2007, Ferrero et al., 2012, Killip and Grewal, 2012)

eif-4E

(Brumby et al., 2004)

eIF-4e

(Gong et al., 2004, Myrick and Dearolf, 1999)

eIF4e

(Ephrussi and St. Johnston, 2004, Khurana et al., 2006)

Eif4e

(Lachance et al., 1996)

Eif4E

eif-4e

(Wessells et al., 2007)

eif4e

(Sofola et al., 2007, Graham et al., 2011)

EIF4E

(Sofola et al., 2007)

eIF 4E

(Ducat et al., 2008)

eIF4E-1

(Hernandez et al., 2006, Hernandez et al., 2005, Hernandez et al., 2012, Pisa et al., 2009, Hernandez and Vazquez-Pianzola, 2005, Hernandez et al., 2004)

eIF-4E1

(Hernandez and Vazquez-Pianzola, 2005)

eIF4E1

(Baker and Fuller, 2007)

eIF-4E1,2

(Hernadez and Rivera Pomar, 2003)

eIF4E-1,2

(Reyes and Izquierdo, 2008, Vazquez-Pianzola et al., 2007)

eIF4E-1/2

(Hernandez et al., 2004, Tettweiler et al., 2012)

eIF4E-2

(Hernandez et al., 2006, Hernandez et al., 2005, Hernandez et al., 2012, Pisa et al., 2009, Hernandez and Vazquez-Pianzola, 2005)

eIF-4E2

(Hernandez and Vazquez-Pianzola, 2005)

eIF4EI

(Cho et al., 2005)

eIF-4EII

(Nelson et al., 2004)

IF4E

(Mushegian et al., 1998)

l(3)67Af

(Leicht and Bonner, 1988, )

l(3)07238

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999, )

l(3)S025007

l(3)S026009

l(3)S058711

l(3)S091912

Name Synonym

cap binding protein

(Zapata et al., 1991)

eukaryotic initiation factor 4E

(Schoenfeld et al., 2000, Parra-Palau et al., 2005, Yarunin et al., 2011)

Eukaryotic initiation factor 4E

(Hernandez et al., 1995, Gouw et al., 2009, Cammarato et al., 2011)

Eukaryotic translation initiation factor 4E