FlyBase Gene Report: Dmel\Hsp60A

General Information

Symbol

Dmel\Hsp60A

Species

D. melanogaster

Name

Heat shock protein 60A

Annotation Symbol

CG12101

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015245

Gene Model Status

Current

Stock Availability

10 publicly available

Gene Summary

Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions. (UniProt, O02649)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Hsp60, l(1)BP5, heat shock protein 60, Hsp 60, 12

Key Links

Genomic Location

Cytogenetic map

10A4-10A4

Sequence location

X:11,108,482..11,112,308 [+]

Recombination map

1-33

RefSeq locus

NC_004354 REGION:11108482..11112308

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR002423, InterPro:IPR018370

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from sequence or structural similarity with UniProtKB:P19882

(Gene Ontology Curators, 2002-)

enables ATP-dependent protein folding chaperone

inferred from electronic annotation with InterPro:IPR001844

(InterPro Project Members, 2004-)

enables protein-folding chaperone binding

inferred from biological aspect of ancestor with PANTHER:PTN000143509

(GO Reference Genome Project, 2011-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in cellular response to heat

inferred from expression pattern

(Baena-López et al., 2008)

involved_in mitochondrion organization

inferred from mutant phenotype

(Baena-López et al., 2008)

NOT involved_in response to heat

inferred from expression pattern

(San Martin et al., 1995)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in apoptotic mitochondrial changes

inferred from biological aspect of ancestor with PANTHER:PTN000888030

(GO Reference Genome Project, 2011-)

involved_in mitochondrial unfolded protein response

inferred from biological aspect of ancestor with PANTHER:PTN000143510

(GO Reference Genome Project, 2011-)

involved_in protein folding

inferred from electronic annotation with InterPro:IPR018370

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000143677

(GO Reference Genome Project, 2011-)

involved_in protein import into mitochondrial intermembrane space

inferred from biological aspect of ancestor with PANTHER:PTN000143510

(GO Reference Genome Project, 2011-)

involved_in protein refolding

inferred from electronic annotation with InterPro:IPR001844

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in mitochondrial inner membrane

inferred from direct assay

(Baena-López et al., 2008)

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

inferred from direct assay

(San Martin et al., 1995)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in mitochondrial inner membrane

inferred from biological aspect of ancestor with PANTHER:PTN000143509

(GO Reference Genome Project, 2011-)

is_active_in mitochondrial matrix

inferred from biological aspect of ancestor with PANTHER:PTN000143509

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

HEAT SHOCK PROTEIN 60 CHAPERONINS - GROUP I

Protein Family (UniProt)

Belongs to the chaperonin (HSP60) family. (O02649)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Group (FlyBase)

HEAT SHOCK PROTEIN 60 CHAPERONINS - GROUP I -: Group I chaperonins catalyse the ATP-dependent folding of proteins. They are found in prokaryotes and endosymbiotic organelles such as mitochondria and chloroplasts. They assemble into large ring-like structures as typified by bacterial GroEL/GroES system. (Adapted from FBrf0190982 and FBrf0232269).

Protein Function (UniProtKB)

Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.

(UniProt, O02649)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Hsp60A for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O02649)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Hsp60A-RA

FBtr0073434

NM_078560

2357

573

Hsp60A-RB

FBtr0073435

NM_167266

2328

573

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Hsp60A-PA

FBpp0073290

60.8

573

5.11

FBpp0073291

60.8

573

5.11

O02649

NP_727489

AAF47998

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

573 aa isoforms: Hsp60A-PA, Hsp60A-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

O02649

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hsp60A using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

2.42

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

Comment: rapidly degraded

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

primordial germ cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

foregut primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

salivary gland body primordium

Comment: reported as salivary gland primordium

(Fisher et al., 2012)

somatic muscle primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 13 -- 16

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic anal pad

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic large intestine

(Fisher et al., 2012)

embryonic Malpighian tubule

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

mitochondrion

(Alonso et al., 2005)

Subcellular Localization

CV Term

Evidence

References

located_in mitochondrial inner membrane

inferred from direct assay

(Baena-López et al., 2008)

located_in mitochondrion

inferred from high throughput direct assay

(Tan et al., 2009, Alonso et al., 2005)

inferred from direct assay

(San Martin et al., 1995)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Hsp60A in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Hsp60A

View exonic expression by tissue for Dmel\Hsp60A

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015245

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32045/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015245

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015245

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015245

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12101

Flygut - An atlas of the Drosophila adult midgut

FBgn0015245

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Hsp60A

Stages(s) 4-6
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 52 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Hsp60A

Transgenic constructs containing regulatory region of Hsp60A

Aberrations (Deficiencies and Duplications) ( 40 )

Inferred from experimentation ( 40 )

Gene disrupted in

Df(1)v65b

(Kozlova et al., 1994, Zhimulev et al., 1981)

Df(1)v-L1

(Kozlova et al., 1994, Pokholkova et al., 1991, Zhimulev et al., 1987, Bgatov et al., 1986, Kramers et al., 1983, Zhimulev, 1982, Zhimulev et al., 1981)

Df(1)v-L3

(Kozlova et al., 1994, Pokholkova et al., 1991, Zhimulev et al., 1987, Kramers et al., 1983, Zhimulev et al., 1981)

Gene duplicated in

Dp(1;2)v⁺63i

(Zhimulev et al., 1987, Kramers et al., 1983, Zhimulev et al., 1981)

Dp(1;2)v⁺65b

(Zhimulev et al., 1987, Geer et al., 1983, Kramers et al., 1983)

Dp(1;2)v⁺75d

(Geer et al., 1983)

Dp(1;Y)v⁺y⁺366

(Zhimulev et al., 1987)

Dp(1;Y)v⁺y⁺

(Zhimulev et al., 1987, Geer et al., 1983, Kramers et al., 1983, Zhimulev et al., 1981)

Gene not disrupted in

Df(1)59D3

(Kozlova et al., 1994, Zhimulev et al., 1987)

Df(1)203C10

(Kozlova et al., 1994, Zhimulev et al., 1987)

Df(1)HC133

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)HM456

(Kramers et al., 1983)

Df(1)KA7

(Zhimulev et al., 1987, Geer et al., 1983, Zhimulev et al., 1981)

Df(1)N71

(Zhimulev et al., 1987, Geer et al., 1983, Zhimulev et al., 1981)

Df(1)RA37

(Kozlova et al., 1994, Pokholkova et al., 1991, Zhimulev et al., 1987, Geer et al., 1983, Kramers et al., 1983, Zhimulev et al., 1981)

Df(1)ras-P14

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)ras-v17

(Kozlova et al., 1994, Pokholkova et al., 1991, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)ras-v-P26

(Zhimulev et al., 1987)

Df(1)sbr1

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)sbr8

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)sbr9

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)sbr10

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)sev-P1

(Kozlova et al., 1994)

Df(1)v64f

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-B151

(Zhimulev et al., 1987)

Df(1)v-L2

(Kozlova et al., 1994, Pokholkova et al., 1991, Zhimulev et al., 1987, Geer et al., 1983, Zhimulev et al., 1981)

Df(1)v-L4

(Kozlova et al., 1994, Zhimulev et al., 1987, Geer et al., 1983, Kramers et al., 1983, Zhimulev et al., 1981)

Df(1)v-L7

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-L11

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-L15

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-M1

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-M5

(Kozlova et al., 1994, Pokholkova et al., 1991, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-M6

(Kozlova et al., 1994, Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-M7

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-P5

(Zhimulev et al., 1987, Zhimulev et al., 1981)

Df(1)v-P16

(Kozlova et al., 1994, Zhimulev et al., 1987)

Df(1)v-P22

(Kozlova et al., 1994, Zhimulev et al., 1987)

In(1)w^m4hrx500

(Kozlova et al., 1994)

Gene not duplicated in

Dp(1;Y)dsh⁺y⁺

(Zhimulev et al., 1987)

Dp(1;Y)v⁺y⁺U15

(Zhimulev et al., 1987)

Inferred from location ( 7 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | germline clone

Hsp60A^DA514

lethal, with Scer\GAL4^Act5C.PI

Hsp60A^GD7481

lethal, with Scer\GAL4^pnr-MD237

Hsp60A^GD7481

lethal, with Scer\GAL4^tin.CΔ4

Hsp60A^GD7481

lethal | cold sensitive

Hsp60A^HM21

lethal | heat sensitive

Hsp60A^F230

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Hsp60A^GD7481

lethal | recessive

lethal | recessive | heat sensitive

Hsp60A^F439

lethal - all die before end of larval stage

Hsp60A^L12

lethal - all die before end of larval stage | recessive

lethal - all die before end of P-stage, with Scer\GAL4^ey.PH

Hsp60A^GD7481

lethal - all die during embryonic stage | recessive

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

Hsp60A^GD7481

partially lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Hsp60A^GD7481

Other Phenotypes

Allele

some die during embryonic stage | recessive

some die during larval stage

Hsp60A^L12

some die during larval stage | recessive

wild-type

Hsp60A^+t15

Phenotype manifest in

Allele

abdominal tergite

embryo | blastoderm stage

Hsp60A^F409

microtubule | third instar larval stage, with Scer\GAL4^elav-C155

Hsp60A^GD7481

neuromuscular junction | third instar larval stage, with Scer\GAL4^elav-C155

Hsp60A^GD7481

terminal bouton | third instar larval stage, with Scer\GAL4^elav-C155

Hsp60A^GD7481

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (11)

Hsap\HSPD1

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\Hspd1

14 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (11)

Mmus\Hspd1

13 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (6)

Xtro\hspd1

11 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (7)

Drer\hspd1

14 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (8)

Cele\hsp-60

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP004002

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (19)

Atha\HSP60

13 of 13

Yes

Atha\HSP60-2

12 of 13

Atha\HSP60-3A

12 of 13

Yes

Atha\CPN60A

6 of 13

Yes

Atha\CPN60B

6 of 13

Yes

Atha\Cpn60beta2

6 of 13

Yes

Atha\Cpn60beta3

6 of 13

Yes

Atha\Cpn60alpha2

5 of 13

Yes

Atha\Cpn60beta4

5 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (10)

Scer\HSP60

13 of 13

Yes

Scer\TCM62

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (8)

Spom\mcp60

12 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\groL

10 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Hsp60A. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (12)

11 of 13

11 of 13

5 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 2 )

Human Ortholog

Disease

Evidence

References

HSPD1; heat shock protein family D (Hsp60) member 1

model of hypomyelinating leukodystrophy 4

IEA

(FlyBase, 2019-)

model of hereditary spastic paraplegia 13

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

Hsp60A^EY01572

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Shirasaki et al., 2012)

Hsp60A^UAS.cPa

ameliorates neurodegenerative disease

modeled by Afg3l2^GD3606

(Pareek and Pallanck, 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

HSPD1; heat shock protein family D (Hsp60) member 1

14 of 14

118190

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Hsp60A - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Hsp60A - Ar2

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Atg4a

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG10927

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG1532

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG16959

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG1910

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG3306

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG4461

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG4949

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG5902

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG7737

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG9008

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG9377

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - CG9822

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Cyp6a13

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - D2hgdh

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Df31

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Flad1

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Hmgs

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Hsp60C

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Ilk

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - JMJD6

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Mettl2

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Pdi

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - PheRS-m

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Raf

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Hsp60A - Rex2

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Rim2

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - S-Lap3

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Shawn

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - Synd

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - al

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Hsp60A - awd

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - cnk

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Hsp60A - csw

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Hsp60A - dnk

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - ex

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Hsp60A - igl

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - ksr

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Hsp60A - mtTFB1

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - nwk

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - regucalcin

experimental knowledge based

(Guruharsha et al., 2011)

Hsp60A - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Hsp60A - rl

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Afg3l2

suppressible

Hsp60A

(Pareek and Pallanck, 2020)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

58460

DroID - A comprehensive database of gene and protein interactions.

FBgn0015245

MIST (protein-protein) - An integrated Molecular Interaction Database

32045

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

RNA degradation

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Mitochondrial protein degradation

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-33

Cytogenetic map

10A4-10A4

Sequence location

X:11,108,482..11,112,308 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

10A4-10A4

Limits computationally determined from genome sequence between P{EP}EP1321^EP1321&P{EP}CG2061^EP1537 and P{EP}CG11756^EP1610

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

10A4-10A5

(Zurita, 1996.7.6)

10A1-10A11

(Caggese, 1996.10.24)

10A1-10A4

(determined by in situ hybridisation)

(Caggese et al., 1999)

10A4-10A5

(determined by in situ hybridisation)

(Zurita et al., 1995)

Experimentally Determined Recombination Data

Location

1-33

(FlyBase, 2016)

1-34.8

(Comeron et al., 2012)

(Caggese, 1996.10.24)

1-33.47

(Geer et al., 1983)

1-33.51

Left of (cM)

l(1)10Ae
rtv

(Zhimulev et al., 1981)

Right of (cM)

(Zhimulev et al., 1981)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

4689

T(1;2;3)RA75, Hsp60A^RA75/FM7a

4686

T(1;2)GF319, l(1)8Aa³ Hsp60A^GF319/FM7a

15361

y¹ w^67c23 P{EPgy2}Hsp60A^EY01572

76428

y¹ sc^* v¹ sev²¹; P{TKO.GS00599}attP40

34729

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01209}attP2/TM3, Sb¹

Kyoto

107838

Hsp60A^RA75/FM7a

107836

T(1;2)GF319, l(1)8Aa³ Hsp60A^GF319/FM7a

12101R-3

v100697

v18738

w¹¹¹⁸; P{GD7481}v18738

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (317)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

SD06594

Other clones

FI05241

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015245

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015245

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Baena-López et al., 2008, Alonso et al., 2005)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene results in a change in cell proliferation and cell size.

(Guertin et al., 2006)

Sequence analysis of l(1)10Ac locus suggests it encodes Hsp60. Molecular analysis of a P-element insertion and genetic rescue experiments confirm l(1)10Ac encodes Hsp60. Hsp60 is differentially expressed throughout development, protein distribution is very dynamic during embryogenesis accumulating in specific cell types.

(Kozlova et al., 1997)

Hsp60 is essential from the early stages of embryogenesis.

(Kozlova et al., 1997)

A protein crossreacting to mammalian CPN60 is detected in the mitochondria of SL2 cells.

(San Martin et al., 1995)

Relationship to Other Genes

Source for database merge of

Additional comments

The Hsp60B gene may have been derived from the Hsp60 gene by retroposition.

(Dai et al., 2006)

The Hsp60C gene may be derived from the Hsp60 gene by retroposition.

(Betran et al., 2002)

May correspond to the l(1)10Ac locus.

(Zurita et al., 1995)

Complementation relations among complementing alleles complex and vary according to temperature of rearing, some combinations being heat sensitive and others cold-sensitive. Allelism to Hsp60^L12 established for the Russian alleles by Zhimulev et al. and for the G8 alleles by Geer et al. (1983).

Nomenclature History

Source for database identify of

Source for identity of: Hsp60A Hsp60

(FlyBase, 2009-)

Nomenclature comments

Renamed from 'Hsp60' to 'Hsp60A' following the nomenclature used in FBrf0190982, and to be consistent with nomenclature of the other three Hsp60 family members (Hsp60B, Hsp60C and Hsp60D).

(FlyBase, 2009-)

Etymology

Synonyms and Secondary IDs (35)

Reported As

Symbol Synonym

(Lefevre and Watkins, 1986, Lefevre, 1981)

BP5

(Zurita et al., 1995)

Cpn60

(San Martin et al., 1995)

Dm10A

(Lakhotia et al., 2002)

DmHsp60

(Wang et al., 2014)

Dmhsp60

(Perezgasga et al., 1999)

G62

(Kramers et al., 1983)

HSP60

(Shiba-Fukushima et al., 2017, Doganlar and Doganlar, 2015, Li et al., 2012, Rera et al., 2011, Shaw et al., 2002)

HSP60A

(Wang et al., 2019, Deng et al., 2018, Deng et al., 2015, Baena-López et al., 2008)

Hsp 60

(Baqri et al., 2014)

Hsp60

(Whitmore et al., 2025, Jia et al., 2022, Ibaraki et al., 2021, Liu et al., 2020, Gumeni et al., 2019, Metwally et al., 2019, Sørensen et al., 2019, Liu et al., 2018, Pareek et al., 2018, Laflamme et al., 2017, Hosamani et al., 2016, Lee et al., 2016, Morrow et al., 2016, Völker-Albert et al., 2016, Chen et al., 2015, Dent et al., 2015, Tsou et al., 2015, Wójtowicz et al., 2015, Chen et al., 2014, Shiba-Fukushima et al., 2014, Zhimulev et al., 2014, Colinet et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Owusu-Ansah et al., 2013, Zhang et al., 2013, Bayat et al., 2012, Fredriksson et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Shirasaki et al., 2012, Friedman et al., 2011, Sun et al., 2011, Anholt and Williams, 2010, Colinet et al., 2010, Babenko et al., 2009, Takemori and Yamamoto, 2009, Tan et al., 2009, Bettencourt et al., 2008, Chen et al., 2008, Fisher et al., 2008, Laayouni et al., 2007, Langille and Clark, 2007, Beller et al., 2006, Beller et al., 2006, Cermelli et al., 2006, Guertin et al., 2006, Mack et al., 2006, Walser et al., 2006, Alonso et al., 2005, Birch-Machin et al., 2005, Riedl et al., 2005, Rehwinkel et al., 2004)

Hsp60A

(Taylor et al., 2024, Vesala et al., 2024, Muita and Baxter, 2023, Yamada et al., 2023, Periasamy et al., 2022, Perlegos et al., 2022, Pun et al., 2022, Faragó et al., 2021, Aboukilila et al., 2020, Hung et al., 2020, Pareek and Pallanck, 2020, Seddigh, 2019, Transgenic RNAi Project members, 2017-, Grant et al., 2010, Sarkar and Lakhotia, 2005)

Hsp64

(Lakhotia et al., 2002, Lakhotia and Singh, 1996)

IEF16

(Baena-López et al., 2008)

Mmp-P1

SSP 7506

(Baena-López et al., 2008)

cpn60

(San Martin et al., 1995)

d-hsp60

(Adan et al., 2008)

hsp60

(Santos-Cruz et al., 2023, Joy et al., 2021, Hill et al., 2018, Sharma et al., 2018, Pandey et al., 2014, Kuo et al., 2013, Singh et al., 2010, Norry et al., 2009, Singh et al., 2009, Robertson et al., 2007, Frydman et al., 2006, Alonso et al., 2005, Moussian et al., 2005, Gorska-Andrzejak et al., 2003, Kang et al., 2002, Karunanithi et al., 1999, Perezgasga et al., 1998, Zurita, 1996.7.6)

hsp60A

(Murari et al., 2022, Baena-López et al., 2008)

l(1)10Ac

l(1)BP5

(Pokholkova et al., 1991, Pokholkova and Solov'eva, 1989, Zhimulev et al., 1987, Bgatov et al., 1986, Pokholkova and Zhimulev, 1984, Zhimulev, 1982, Zhimulev et al., 1981)

l(1)G8

l(1)HM21

l(1)L12

l(1)dp025

Name Synonyms

60-kDa heat shock protein, mitochondrial

(Pflieger et al., 2008)

HEAT SHOCK PROTEIN 60

(Alonso et al., 2005)

Heat shock protein 60

(Cammarato et al., 2011, Cermelli et al., 2006, Zurita et al., 1995)

Heat shock protein 60A

(Liu et al., 2018)

Hsp 60

(Takemori and Yamamoto, 2009)

Mitochondrial matrix protein P1

heat shock protein 60

(Breloy et al., 2016, Sun et al., 2011, Baena-López et al., 2008, Mack et al., 2006)

heat shock protein 60 kDa

(Caggese, 1996.10.24)

lethal(1)10Ac

Secondary FlyBase IDs

FBgn0001483
FBgn0001749
FBgn0004659
FBgn0010375

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 51 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

32045

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O02649

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O02649

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O02649

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015245

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32045/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015245

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015245

FlyMine - An integrated database for Drosophila genomics

FBgn0015245

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG12101

MARRVEL_MODEL - MARRVEL (model organism gene)

32045

Linkouts

BioGRID - A database of protein and genetic interactions.

58460

Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.

4869
4870

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015245

DroID - A comprehensive database of gene and protein interactions.

FBgn0015245

DRSC - Results frm RNAi screens

FBgn0015245

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015245

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015245

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12101

Flygut - An atlas of the Drosophila adult midgut

FBgn0015245

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013683

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

RNA degradation

MIST (protein-protein) - An integrated Molecular Interaction Database

32045

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Mitochondrial protein degradation

References (202)

The following external sites may use different assemblies or annotations than FlyBase.

2.42

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