FlyBase Gene Report: Dmel\Diap2

General Information

Symbol

Dmel\Diap2

Species

D. melanogaster

Name

Death-associated inhibitor of apoptosis 2

Annotation Symbol

CG8293

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015247

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Summary

Death-associated inhibitor of apoptosis 2 (Diap2) encodes a protein with ubiquitin E3-ligase activity that functions in the Imd pathway by conjugating the product of imd with K63-polyubiquitin chains. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

Iap2, DIAP-2, DIAP, DIHA, IAP-like protein

Key Links

Genomic Location

Cytogenetic map

52D2-52D2

Sequence location

2R:15,932,170..15,934,825 [-]

Recombination map

2-76

RefSeq locus

NT_033778 REGION:15932170..15934825

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

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Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (22 terms)

Molecular Function (6 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

enables caspase binding

inferred from physical interaction with FLYBASE:Drice; FB:FBgn0019972

(Leulier et al., 2006)

enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process

inferred from direct assay

(Ribeiro et al., 2007)

enables protein binding

inferred from physical interaction with FLYBASE:rpr; FB:FBgn0011706

(Vucic et al., 1997)

enables ubiquitin protein ligase activity

inferred from direct assay

(Aalto et al., 2019, Yeh and Bratton, 2013, Paquette et al., 2010, Herman-Bachinsky et al., 2007, Ribeiro et al., 2007)

enables ubiquitin protein ligase binding

inferred from physical interaction with FLYBASE:Diap1; FB:FBgn0260635

(Herman-Bachinsky et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables cysteine-type endopeptidase inhibitor activity involved in apoptotic process

inferred from biological aspect of ancestor with PANTHER:PTN000953271

(assigned by GO_Central )

(Gaudet et al., 2011)

enables ubiquitin protein ligase activity

inferred from biological aspect of ancestor with PANTHER:PTN000799425

(assigned by GO_Central )

(Gaudet et al., 2011)

enables zinc ion binding

inferred from sequence model

(Ying et al., 2011)

Biological Process (13 terms)

Terms Based on Experimental Evidence (11 terms)

CV Term

Evidence

References

involved_in chaeta development

inferred from mutant phenotype

(Kanuka et al., 2005)

involved_in defense response to Gram-negative bacterium

inferred from mutant phenotype

(Meinander et al., 2012, Huh et al., 2007, Leulier et al., 2006)

involved_in negative regulation of apoptotic process

inferred from direct assay

(Hawkins et al., 1998, Vucic et al., 1997)

inferred from mutant phenotype

(Ribeiro et al., 2007)

involved_in negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

inferred from genetic interaction with FLYBASE:Drice; FB:FBgn0019972

(Ribeiro et al., 2007)

inferred from direct assay

(Harvey et al., 2001)

involved_in peptidoglycan recognition protein signaling pathway

inferred from mutant phenotype

(Chen et al., 2017, Meinander et al., 2012, Paquette et al., 2010, Ertürk-Hasdemir et al., 2009, Valanne et al., 2007, Leulier et al., 2006, Kleino et al., 2005)

inferred from high throughput mutant phenotype

(Kleino et al., 2005)

involved_in positive regulation of biosynthetic process of antibacterial peptides active against Gram-negative bacteria

inferred from mutant phenotype

(Kleino et al., 2005)

inferred from high throughput mutant phenotype

(Kleino et al., 2005)

involved_in protein K48-linked ubiquitination

inferred from direct assay

(Herman-Bachinsky et al., 2007)

involved_in protein K63-linked ubiquitination

inferred from direct assay

(Aalto et al., 2019, Meinander et al., 2012)

involved_in protein ubiquitination

inferred from direct assay

(Yeh and Bratton, 2013)

involved_in regulation of cysteine-type endopeptidase activity involved in apoptotic process

inferred from mutant phenotype

(Leulier et al., 2006)

involved_in ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Herman-Bachinsky et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in negative regulation of apoptotic process

inferred from biological aspect of ancestor with PANTHER:PTN000953271

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in positive regulation of protein ubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000799425

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in regulation of cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN000005168

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in perinuclear region of cytoplasm

inferred from direct assay

(Vucic et al., 1997)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000005166

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000005166

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

INHIBITOR OF APOPTOSIS UBIQUITIN LIGASES

INHIBITOR OF APOPTOSIS

Pathway (FlyBase)

Imd Signaling Pathway Core Components

Protein Family (UniProt)

Belongs to the IAP family. (Q24307)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

INHIBITOR OF APOPTOSIS UBIQUITIN LIGASES -: E3 (ubiquitin) ligases interact with both ubiquitin-E2 (ubiquitin-conjugating) enzymes and the target protein, transferring the ubiquitin from the E2 to the substrate. Inhibitor of APoptosis (IAP) ubiquitin ligases are defined by the presence of one or more BIR (baculoviral IAP repeat) and a UBA (ubiquitin (Ub)-associated domain) domain that binds polyubiquitin and a RING finger domain. (Adapted from FBrf0216918).
INHIBITOR OF APOPTOSIS -: Inhibitor of APoptosis (IAP) proteins are defined by the presence of one or more BIR (baculoviral IAP repeat) domains. Not all IAPs inhibit apoptosis, but some interact with and inhibit caspases via their BIR domains. This interaction is displaced by RHG protein binding. (Adapted from FBrf0215539).

Pathway (FlyBase)

Imd Signaling Pathway Core Components -: The immune deficiency (Imd) pathway primarily mediates the humoral immune response to Gram-negative bacteria. Activation of the Imd pathway by diaminopimelic acid-type peptidoglycan initiates a signaling cascade that ultimately results in the release of the NFκB-like factor Rel from auto-inhibition and its translocation into the nucleus to activate the transcription of antimicrobial peptides. (Adapted from FBrf0224587 and FBrf0238555.)

Protein Function (UniProtKB)

Required for activation of NF-kappaB transcription factors in the immune deficiency (Imd) signaling cascade which is essential for innate immune responses upon infection by Gram-negative bacteria (PubMed:16894030, PubMed:17068333). Promotes cytoplasmic cleavage of Rel and its translocation to the nucleus where it drives expression of antimicrobial peptides (PubMed:17068333, PubMed:24374974). Binds, polyubiquitinates and activates Dredd which is required for Rel-mediated induction of antimicrobial peptides (PubMed:22549468). Anti-apoptotic protein which binds, ubiquitinates and inactivates the effector caspase Drice (PubMed:18166655). Suppresses rpr and hid-dependent cell death in the eye (PubMed:8548811). However, has also been shown to have little, if any, role in the regulation of the canonical caspase-dependent apoptosis pathway (PubMed:17068333). Plays a role in regulating the expression of ion channels (PubMed:24374974).

(UniProt, Q24307)

Summary (Interactive Fly)

N-terminal baculovirus IAP repeat domain and C-terminal RING ubiquitin ligase domain protein - required for immune response to bacteria - promotes cytoplasmic cleavage and nuclear translocation of the NF-kappaB homolog Relish - controls the level of caspase activity by forming a covalent adduct with the catalytic machinery of drICE

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Diap2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q24307)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Diap2-RA

FBtr0087296

NM_057779

2106

498

Diap2-RB

FBtr0087297

NM_176182

1997

498

Additional Transcript Data and Comments

Reported size (kB)

2.3 (northern blot)

(Duckett et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Diap2-PA

FBpp0086431

54.5

498

4.91

NP_477127

AAF58095

Diap2-PB

FBpp0086432

54.5

498

4.91

NP_788362

AAO41389

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

498 aa isoforms: Diap2-PA, Diap2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

497 (aa); 54 (kD predicted)

(Duckett et al., 1996)

498 (aa)

(Liston et al., 1996, Hay et al., 1995)

498 (aa); 55 (kD predicted)

(Uren et al., 1996)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts with the caspase Strica (PubMed:11550090). Interacts (via BIR2 domain) with rpr and grim (PubMed:18166655). Interacts (via the BIR2 and BIR3 domains) with hid (PubMed:18166655). Interacts (via BIR3 domain) with Drice (PubMed:18166655). Interacts with Dredd; likely to bind Dredd simultaneously with Fadd to form a trimeric complex (PubMed:22549468).

(UniProt, Q24307)

Post Translational Modification

Caspase-dependent cleavage is required for suppression of Drice-mediated cell death.

(UniProt, Q24307)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q24307

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Diap2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Diap2_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage & larval stage & adult stage

(Duckett et al., 1996)

Additional Descriptive Data

Iap2 transcripts are expressed at high levels in embryos, larvae, and adults.

(Duckett et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- adult stage

Malpighian tubule

(Verma and Tapadia, 2014)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in perinuclear region of cytoplasm

inferred from direct assay

(Vucic et al., 1997)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Diap2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Diap2

View exonic expression by tissue for Dmel\Diap2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015247

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36748/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015247

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015247

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015247

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8293

Flygut - An atlas of the Drosophila adult midgut

FBgn0015247

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Diap2

Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 8 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 28 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Diap2

Transgenic constructs containing regulatory region of Diap2

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(2R)Exel7138

(Leulier et al., 2006, Ryder, 2004.4.26)

Df(2R)Jp1

(Colussi et al., 2000)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of pupal stage, with Scer\GAL4^da.G32

Diap2^dsRNA.UAS

partially lethal - majority die, with Scer\GAL4^pnr-MD237

Diap2^GD1466

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Diap2^GD1466

viable, with Scer\GAL4^Mef2.PR

Diap2^GD1466

viable, with Scer\GAL4^tin.CΔ4

Diap2^GD1466

viable (with Df(2R)Exel7138)

Diap2^7c

Sterility

Allele

fertile

Other Phenotypes

Allele

abnormal body color, with Scer\GAL4^pnr-MD237

Diap2^GD1466

abnormal cell death | conditional

Diap2^7c

abnormal cell death | conditional (with Df(2R)Exel7138)

Diap2^7c

abnormal immune response

abnormal immune response, with Scer\GAL4^c564

Diap2^dsRNA.UAS

abnormal immune response (with Df(2R)Exel7138)

Diap2^7c

abnormal immune response (with Diap2^7a)

Diap2^7c

abnormal immune response (with Diap2^7c)

Diap2^7a

radiation sensitive

Diap2^7c

radiation sensitive (with Df(2R)Exel7138)

Diap2^7c

some die during pupal stage, with Scer\GAL4^da.G32

Diap2^dsRNA.UAS

some die during pupal stage, with Scer\GAL4^pnr-MD237

Diap2^GD1466

visible, with Scer\GAL4^pnr-MD237

Diap2^GD1466

visible, with Scer\GAL4^sca-P309

Diap2^UAS.cIa

Phenotype manifest in

Allele

eye | P-stage

Diap2^GMR.PH

macrochaeta | increased number, with Scer\GAL4^sca-P309

Diap2^UAS.cIa

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Diap2^GD1466

pigment cell

Diap2^GMR.PH

trichogen cell, with Scer\GAL4^pnr-MD237

Diap2^GD1466

wing disc

Diap2^7c

wing disc (with Df(2R)Exel7138)

Diap2^7c

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (14)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\BIRC2

13 of 15

Yes

Hsap\BIRC3

12 of 15

Yes

Hsap\XIAP

11 of 15

Yes

Hsap\BIRC7

7 of 15

Yes

Hsap\BIRC8

5 of 15

Yes

Hsap\NAIP

4 of 15

Yes

3 of 15

2 of 15

1 of 15

1 of 15

Yes

Hsap\F12

1 of 15

Yes

Hsap\GNAS

1 of 15

Hsap\NLRC4

1 of 15

Yes

Hsap\SLC1A5

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (12)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Birc2

14 of 15

Yes

Mmus\Birc3

13 of 15

Yes

Mmus\Xiap

10 of 15

Yes

Mmus\Birc7

6 of 15

Yes

Mmus\Naip1

4 of 15

Yes

Mmus\Naip2

4 of 15

Yes

Mmus\Naip5

4 of 15

Yes

Mmus\Naip6

4 of 15

Yes

3 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (8)

Rnor\Birc2

13 of 13

Yes

Rnor\Birc3

12 of 13

Yes

Rnor\Xiap

10 of 13

Yes

Rnor\Birc7

6 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (11)

Xtro\birc2

9 of 12

Yes

Xtro\birc7

9 of 12

Yes

Xtro\xiap

9 of 12

Yes

Xtro\birc5.1

4 of 12

Xtro\birc5.2

3 of 12

Xtro\LOC101733194

2 of 12

Yes

Xtro\LOC108645627

2 of 12

Yes

Xtro\LOC108648784

2 of 12

Yes

Xtro\birc6

1 of 12

Xtro\mylip

1 of 12

Xtro\nlrc4

1 of 12

Yes

Danio rerio (Zebrafish) (8)

Drer\birc2

14 of 15

Yes

Drer\xiap

6 of 15

Yes

Drer\birc7

5 of 15

Yes

4 of 15

2 of 15

2 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\bir-2

6 of 15

Yes

Cele\K11D12.9

4 of 15

Yes

Cele\bir-1

1 of 15

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\BIR1

2 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\bir1

5 of 12

Yes

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (4)

Gene Symbol

Score

Source

Alignment

5 of 10

2 of 10

Det

2 of 10

dnr1

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

XIAP; X-linked inhibitor of apoptosis

model of X-linked lymphoproliferative syndrome 2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Diap2^GMR.PH

ameliorates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

BIRC2; baculoviral IAP repeat containing 2

13 of 15

601712

BIRC3; baculoviral IAP repeat containing 3

12 of 15

601721

XIAP; X-linked inhibitor of apoptosis

11 of 15

300079

LYMPHOPROLIFERATIVE SYNDROME, X-LINKED, 2; XLP2

X-linked lymphoproliferative syndrome 2

BIRC7; baculoviral IAP repeat containing 7

7 of 15

605737

BIRC8; baculoviral IAP repeat containing 8

5 of 15

NAIP; NLR family apoptosis inhibitory protein

4 of 15

600355

BIRC5; baculoviral IAP repeat containing 5

3 of 15

603352

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Diap2

enzymatic study, western blot

(Yoo, 2005)

Diap2 - CG3262

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - Coprox

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - Dredd

anti tag coimmunoprecipitation, anti tag western blot, pull down

(Guntermann et al., 2015, Meinander et al., 2012, Guntermann and Foley, 2011)

Diap2 - Drice

anti tag coimmunoprecipitation, western blot

(Ribeiro et al., 2007, Leulier et al., 2006)

Diap2 - EndoG

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - EndoGI

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - Fadd

anti tag coimmunoprecipitation, anti tag western blot

(Guntermann et al., 2015, Meinander et al., 2012, Guntermann and Foley, 2011)

Diap2 - Gsc

two hybrid

(Hu et al., 2017.6.13)

Diap2 - HtrA2

protease assay, autoradiography, pull down

(Khan et al., 2008)

Diap2 - Jafrac2

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - Mpcp2

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - PGRP-LC

anti tag coimmunoprecipitation, anti tag western blot

(Neyen et al., 2016)

Diap2 - Su(P)

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - grim

pull down, autoradiography

(Khan et al., 2008)

Diap2 - hid

pull down, anti tag western blot, western blot

(Ribeiro et al., 2007, Leulier et al., 2006)

Diap2 - imd

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot, pull down

(Meinander et al., 2012, Paquette et al., 2010)

Diap2 - mtDNA-helicase

experimental knowledge based

(Guruharsha et al., 2011)

Diap2 - rpr

pull down, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Ribeiro et al., 2007, Vucic et al., 1997)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

AIF

suppressible

Diap2

(Joza et al., 2008)

aos

suppressible

Diap2

(Sawamoto et al., 1998)

Diap1

suppressible

Diap2

(Ribeiro et al., 2007, Leulier et al., 2006)

Dref

suppressible

Diap2

(Hirose et al., 2001)

Dronc

suppressible

Diap2

(Quinn et al., 2000)

grim::rpr

suppressible

Diap2

(Wing et al., 2001)

grim

suppressible

Diap2

(Wing et al., 2001)

hid

suppressible

Diap2

(Wing et al., 1998, Hay et al., 1995)

Rac1

suppressible

Diap2

(Geisbrecht and Montell, 2004)

Rfx2

suppressible

Diap2

(Otsuki et al., 2004)

rpr

suppressible

Diap2

(Wing et al., 1998, Hay et al., 1995)

Syn2

suppressible

Diap2

(Nagai et al., 2010)

External Data

Subunit Structure (UniProtKB)

(UniProt, Q24307 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62475

DroID - A comprehensive database of gene and protein interactions.

FBgn0015247

MIST (genetic) - An integrated Molecular Interaction Database

Diap2

MIST (protein-protein) - An integrated Molecular Interaction Database

Diap2

Pathways

Signaling Pathways (FlyBase)

Imd Signaling Pathway Core Components -: The immune deficiency (Imd) pathway primarily mediates the humoral immune response to Gram-negative bacteria. Activation of the Imd pathway by diaminopimelic acid-type peptidoglycan initiates a signaling cascade that ultimately results in the release of the NFκB-like factor Rel from auto-inhibition and its translocation into the nucleus to activate the transcription of antimicrobial peptides. (Adapted from FBrf0224587 and FBrf0238555.)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-76

Cytogenetic map

52D2-52D2

Sequence location

2R:15,932,170..15,934,825 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

52D2-52D2

Limits computationally determined from genome sequence between P{lacW}l(2)k02205^k02205&P{PZ}sli⁰⁵²⁴⁸ and P{lacW}ATPCL^k09217

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

52D1-52D2

(Hay, 1996.1.5)

52D-52D

(determined by in situ hybridisation)

(Duckett et al., 1996)

52D1-52D2

(determined by in situ hybridisation)

(Hay et al., 1995)

Experimentally Determined Recombination Data

Location

2-76

(FlyBase, 2016)

2-80.0

(Comeron et al., 2012)

(Hay, 1996.1.5)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

5783

w^*; P{GMR-DIAP2}BH1

55725

w^*; Diap2^7c

81801

y¹ v¹; P{TKO.GS04849}attP40

26986

y¹ w^*; P{EP}Diap2^G2326

34776

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00085}attP2

VDRC

v105351

P{KK101187}VIE-260B

v2973

w¹¹¹⁸; P{GD1466}v2973

Genomic Clones (20)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (62)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD34777

Other clones

DPiM_LD34777

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015247

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015247

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36748

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Leulier et al., 2006)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Diap2 Iap2

(Steller et al., 2014.7.9)

Source for database merge of

Additional comments

Changed from 'Iap2' to 'Diap2' to reflect common usage in the literature.

(Steller et al., 2014.7.9)

Other Comments

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a specific decrease in AttA activity in response to heat-killed E.coli when assayed in S2 cells. This effect is independent of ecdysone. Iap2 functions downstream of imd in the fat body, as shown by the ability of Iap2 dsRNA to inhibit transcriptional activation of Dpt by imd. Tab2 RNAi fails to prevent AttA activation by Iap2, indicating that Tab2 is upstream of Iap2 in the imd signalling pathway.

(Kleino et al., 2005)

Iap2 may be involved in Rel nuclear localization.

(Kleino et al., 2005)

The product of Iap2 can inhibit mammalian cell death induced by overexpression of caspases 1 and 2.

(Hawkins et al., 1998)

The Iap2 protein associates with the tkv receptor in vivo.

(Oeda et al., 1998)

Expression of death inducers rpr and W and the repression of death inhibitor Iap2 correlates with the onset of histolysis in the larval salivary gland, suggesting that programmed cell death may be coordinated by both inductive and repressive mechanisms.

(Jiang et al., 1997)

Drosophila homolog of baculovirus iap is molecularly cloned.

(Duckett et al., 1996)

The conservation of IAPs from humans to Drosophila suggests that they are important for the regulation of apoptosis.

(Liston et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 62 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36748

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q24307

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24307

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q24307

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015247

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015247

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36748/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015247

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Diap2_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015247

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015247

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8293

Flygut - An atlas of the Drosophila adult midgut

FBgn0015247

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

92748

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36748

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC001189

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8293

MARRVEL_MODEL - MARRVEL (model organism gene)

36748

modMine - A data warehouse for the modENCODE project

FBgn0015247

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q24307

Linkouts

BioGRID - A database of protein and genetic interactions.

62475

DroID - A comprehensive database of gene and protein interactions.

FBgn0015247

DRSC - Results frm RNAi screens

FBgn0015247

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015247

FlyMine - An integrated database for Drosophila genomics

FBgn0015247

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Diap2

MIST (protein-protein) - An integrated Molecular Interaction Database

Diap2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (29)

Reported As

Symbol Synonym

CG8293

(Hosono et al., 2015, Ni et al., 2011.7.19, Ying et al., 2011, Keleman et al., 2009.8.5, Huh et al., 2007, Kleino et al., 2005, Labourier et al., 2002)

D-IAP2

(Deveraux and Reed, 1999, Miller, 1999)

D-iap2

(Vucic et al., 1998, Vucic et al., 1997, Vucic et al., 1997)

DIAP

(Ziraldo and Ma, 2015, Finelli et al., 2002, White, 1999, Baird, 1996.1.16)

DIAP-2

(Bergmann et al., 2003, Jackson et al., 2002, Wang, 2001, Massagué and Chen, 2000, Zapata et al., 2000, Meier and Evan, 1998)

DIAP1

(Schneider and Imler, 2021)

DIAP2

(Hounsell and Fan, 2021, Aalto et al., 2019, Goto et al., 2018, Zhang et al., 2016, Aparicio et al., 2013, Rallis et al., 2013, Resende et al., 2013, Yeh and Bratton, 2013, Darding and Meier, 2012, Eleftherianos and Castillo, 2012, Vandenabeele and Bertrand, 2012, Kuranaga, 2011, Bader et al., 2010, Nagai et al., 2010, Nezis et al., 2010, Paquette et al., 2010, Cronin et al., 2009, Ertürk-Hasdemir et al., 2009, Khan et al., 2008, Lhocine et al., 2008, Herman-Bachinsky et al., 2007, Ribeiro et al., 2007, Ribeiro et al., 2007, Leulier et al., 2006, Anderson et al., 2005, Dotto and Silke, 2004, Geisbrecht and Montell, 2004, Otsuki et al., 2004, Renault et al., 2004, Shiozaki and Shi, 2004, Yeo and Gautier, 2004, Holcik, 2002, Holcik, 2002, Igaki et al., 2002, Jackson et al., 2002, Martin, 2002, Srinivasula et al., 2002, Tenev et al., 2002, Zimmermann et al., 2002, Doumanis et al., 2001, Hirose et al., 2001, Silke and Vaux, 2001, Hawkins et al., 2000, Igaki et al., 2000, Meier et al., 2000, Ohno et al., 2000, Reed and Bischoff, 2000, Takatsu et al., 2000, Vernooy et al., 2000, Vernooy et al., 2000, Vernooy et al., 2000, Abrams, 1999, Vaux and Korsmeyer, 1999, Foley and Cooley, 1998, Kaiser et al., 1998, LaCasse et al., 1998, Oeda et al., 1998, Wing and Nambu, 1998, Clem and Duckett, 1997, Harvey et al., 1997, Jiang et al., 1997, McCall and Steller, 1997, Chinnaiyan and Dixit, 1996, Hay, 1996.1.5, Hay et al., 1995)

DIAPII

(Anderson et al., 2005)

DIHA

(Hawkins et al., 1998, LaCasse et al., 1998, Uren et al., 1996, Puls, 1995.10.17)

Diap

(Xu et al., 2021)

Diap-2

(Arya and White, 2015)

Diha

IAP

(Nakayama et al., 2000, Conlan and Raff, 1999)

IAP2

(Rosendo Machado et al., 2021, Galenza and Foley, 2019, Kleino and Silverman, 2014, Fukuyama et al., 2013, Yang et al., 2013, Waterhouse et al., 2007, Gesellchen et al., 2005)

Ilp

dIAP

(White, 2000)

dIAP2

(Neyen et al., 2016, Aparicio et al., 2013, Gistelinck et al., 2012, Igboin et al., 2012, Guntermann and Foley, 2011, Bond and Foley, 2009, Silverman et al., 2008, Rawls et al., 2007, Bangs et al., 2000)

dILP

(Grewal, 2012, LaCasse et al., 1998, Duckett et al., 1996)