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Gene Dmel\Nf1

General Information
Symbol	Dmel\Nf1	Species	D. melanogaster
Name	Neurofibromin 1	Annotation symbol	CG8318
Feature type	protein_coding_gene	FlyBase ID	FBgn0015269
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	NF-1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	96F9-96F9	Sequence location	3R:21,808,735..21,821,459 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Neurofibromin 1 is referred to in FlyBase by the symbol Dmel\Nf1 (CG8318, FBgn0015269). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: Ras GTPase activator activity. There is experimental evidence for 14 unique biological process terms, many of which group under: biological regulation; response to stimulus; learning or memory; response to stress; system process; intracellular signal transduction; regulation of multicellular organismal process; cognition; cellular process; associative learning; determination of adult lifespan; growth; locomotory behavior; short-term memory. 36 alleles are reported. The phenotypes of these alleles are annotated with: pupa; wing disc; surface associated glial cell; heart primordium; eye photoreceptor cell; wing. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Armadillo-type fold; Cellular retinaldehyde-binding/triple function, C-terminal; Ras GTPase-activating protein; Rho GTPase activation protein. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. Gene sequence location is 3R:21808735..21821459.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Gouzi et al., 2011, PLoS Genet. 7(9): e1002281
FB2012_01	Sequence features BKN21975 HFA16758
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 96F9-96F9   Limits computationally determined from genome sequence between P{PZ}l(3)rQ197rQ197 and P{lacW}scribj7B3
Experimentally Determined Cytological Location
Cytogenetic map Notes References 96F-96F     (Bernards, 1997.4.8) 96F-96F   (determined by in situ hybridisation)   (The et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nf1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nf1-RB FBtr0084952  NM_170253   8893   2802 Nf1-RC FBtr0084953  NM_170252   8892   2746 Nf1-RD FBtr0100113     9737   2764
Additional Transcript Data & Comments
Reported size (kB)	9.750, 8.843 (compiled cDNA) (The et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nf1-PB   FBpp0084326   317.2   2802   6.86     NP_733132   AAF56543 Nf1-PC   FBpp0084327   311.1   2746   6.74     NP_733131   AAN14067 Nf1-PD   FBpp0100151   313.0   2764   6.80     NP_001014668   AAX52994
Additional Polypeptide Data & Comments
Reported size (kDa)	2802, 2764 (aa); 280 (kD observed) (The et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Cellular retinaldehyde-binding/triple function, C-terminal (IPR001251) Ras GTPase-activating protein (IPR001936) Rho GTPase activation protein (IPR008936) Armadillo-type fold (IPR016024)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56543   AE014297 AAN14067   AE014297 AAX52994   AI109596   AJ276314   AW941718   AY058319 AAL13548   BI563967   BT024936   CK135248   CO177793   CO178204   CO189529   CO262387   CO274608   CO274700   CO286697   CO289473   CO290425   CO293024   CO298643   CO313761   CO317545   CO320998   CO322181   CO323309   CO341124   CZ466483   CZ466605   CZ468345   CZ477989   EC057840   EC060605   EC068988   EC196663   EC199765   EC218576   EC219340   EC219341   EC230818   EC232628   EC237270   EC238648   EC242141   EC244466   EC250091   EC258242   EL885240   EL885241   L26500 AAB58977   L26501 AAB58975   L26502 AAB58976
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O01397 O01398 O01399 Q8IMS2 Q95U43 Q9VBJ2 Q59DT9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (The et al., 1997) larval stage   imaginal disc   (The et al., 1997) adult stage   Kenyon cell | subset   (Buchanan and Davis, 2010) adult brain cortex   (Buchanan and Davis, 2010)
Additional Descriptive Data
	Nf1 is expressed in most, if not all, cell body regions of the adult central brain. Expression is apparent in cell body regions surrounding the antennal lobes, protocerebrum, lateral horn and the mushroom body calyces. (Buchanan and Davis, 2010) Nf1 transcripts are widely expressed in low amounts. (The et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (The et al., 1997) larval stage   imaginal disc   (The et al., 1997)
Additional Descriptive Data
	Nf1 protein is widely expressed in low amounts. (The et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0015269    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0015269 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of low expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0015269 expression level Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (779) embryo 02-04hr  (619) embryo 04-06hr  (596) embryo 06-08hr  (510) embryo 08-10hr  (613) embryo 10-12hr  (271) embryo 12-14hr  (680) embryo 14-16hr  (573) embryo 16-18hr  (586) embryo 18-20hr  (480) embryo 20-22hr  (603) embryo 22-24hr  (772) larva L1  (528) larva L2  (344) larva L3 12hr old  (245) larva L3 puffstage 1-2  (185) larva L3 puffstage 3-6  (470) larva L3 puffstage 7-9  (527) white prepupae new  (463) white prepupae 12hr  (481) white prepupae 24hr  (704) pupae 2d postWPP  (737) pupae 3d postWPP  (563) pupae 4d postWPP  (372) adult male 01day  (686) adult male 05day  (802) adult male 30day  (760) adult female 01day  (445) adult female 05day  (706) adult female 30day  (651) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0015269 expression level Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (779) embryo 02-04hr  (619) embryo 04-06hr  (596) embryo 06-08hr  (510) embryo 08-10hr  (613) embryo 10-12hr  (271) embryo 12-14hr  (680) embryo 14-16hr  (573) embryo 16-18hr  (586) embryo 18-20hr  (480) embryo 20-22hr  (603) embryo 22-24hr  (772) larva L1  (528) larva L2  (344) larva L3 12hr old  (245) larva L3 puffstage 1-2  (185) larva L3 puffstage 3-6  (470) larva L3 puffstage 7-9  (527) white prepupae new  (463) white prepupae 12hr  (481) white prepupae 24hr  (704) pupae 2d postWPP  (737) pupae 3d postWPP  (563) pupae 4d postWPP  (372) adult male 01day  (686) adult male 05day  (802) adult male 30day  (760) adult female 01day  (445) adult female 05day  (706) adult female 30day  (651) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    779 embryo 02-04hr    619 embryo 04-06hr    596 embryo 06-08hr    510 embryo 08-10hr    613 embryo 10-12hr    271 embryo 12-14hr    680 embryo 14-16hr    573 embryo 16-18hr    586 embryo 18-20hr    480 embryo 20-22hr    603 embryo 22-24hr    772 larva L1    528 larva L2    344 larva L3 12hr old    245 larva L3 puffstage 1-2    185 larva L3 puffstage 3-6    470 larva L3 puffstage 7-9    527 white prepupae new    463 white prepupae 12hr    481 white prepupae 24hr    704 pupae 2d postWPP    737 pupae 3d postWPP    563 pupae 4d postWPP    372 adult male 01day    686 adult male 05day    802 adult male 30day    760 adult female 01day    445 adult female 05day    706 adult female 30day    651 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0015269    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0015269 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Two or more Affy2 ProbeSets identify exons of this gene. This is a summary of the tissue expression peaks exhibited in at least one of these ProbeSets. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Heatmap Tissue   Expression Level Larval Central Nervous System no informative data Larval Midgut     Larval Hindgut no informative data Larval Malpighian Tubules no informative data Larval Fat Body     Larval Salivary Gland no informative data Larval Trachea no informative data Larval Carcass no informative data Adult Head no informative data Adult Eye no informative data Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop no informative data Adult Midgut     Adult Hindgut no informative data Adult Malpighian Tubules     Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary no informative data Adult Testis no informative data Adult Male Accessory Gland no informative data Adult Carcass     modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_27
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015269 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8318-RB;CG8318-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable Nf1c00617 Nf1P1 Nf1P2 viable, with Scer\GAL4pnr-MD237 Nf1GD13892 Sterility Allele fertile Nf1c00617 Nf1P1 Nf1P2 semi-sterile, with Nf1P1, Scer\GAL4c305a Nf1Scer\UAS.cBa semi-sterile, with Nf1Scer\UAS.cBa, Scer\GAL4c305a Nf1P1 semi-sterile (with Nf1P1), with Scer\GAL4c305a Nf1Scer\UAS.cBa semi-sterile (with Nf1Scer\UAS.cBa), with Scer\GAL4c305a Nf1P1 Other Phenotypes Allele behavior defective Nf1P1 Nf1P2 decreased cell size | adult stage Nf1E2 learning defective Nf1P1 Nf1P2 learning defective | adult stage (with Nf1E1) Nf1E2 learning defective | adult stage (with Nf1E2) Nf1E1 learning defective | adult stage | dominant Nf1E2 locomotor rhythm defective Nf1P1 Nf1P2 Nf1unspecified locomotor rhythm defective (with Df(3R)Espl3) Nf1P1 Nf1P2 memory defective Nf1c00617 Nf1P1 Nf1P2 size defective Nf1unspecified size defective | dominant | pupal stage Nf1E2 size defective | pupal stage (with Nf1E1) Nf1E2 size defective | pupal stage (with Nf1E2) Nf1E1 small body Nf1P1 Nf1P2 small body | male limited Nf1c00617 small body | pupal stage Nf1E1 Nf1E2 small body | pupal stage (with Nf1E1) Nf1P2 small body | pupal stage (with Nf1E2) Nf1P2 small body | pupal stage (with Nf1E3) Nf1P2 small body | pupal stage (with Nf1E4) Nf1P2 small body | pupal stage (with Nf1P2) Nf1E1 Nf1E2 Nf1E3 Nf1E4 small body | pupal stage | heat sensitive Nf1E4 wild-type Nf1hs.PT Phenotype manifest in Allele eye photoreceptor cell Nf1P1 Nf1P2 heart primordium Nf1dsRNA.cKa Nf1dsRNA.si.cKa neuromuscular junction | larval stage Nf1P1 Nf1P2 pupa Nf1E1 Nf1E2 Nf1E4 pupa (with Nf1E1) Nf1P2 pupa (with Nf1E2) Nf1P2 pupa (with Nf1E4) Nf1P2 pupa (with Nf1P2) Nf1E1 Nf1E2 Nf1E4 surface associated glial cell Nf1P2 wing Nf1E1 Nf1E2 Nf1E4 wing disc Nf1E1
Classical Alleles ( 12 )
For All Classical Alleles Show
Allele of Nf1 Class Mutagen Stocks Known lesion Nf1c00617 piggyBac transposase 2 -- Nf1c02945 piggyBac transposase 1 -- Nf1e00084 piggyBac transposase 1 -- Nf1MI01388 1 -- Nf1c00472 piggyBac transposase 0 -- Nf1E1 ethyl methanesulfonate 0 Yes Nf1E2 ethyl methanesulfonate 0 Yes Nf1E3 ethyl methanesulfonate 0 Yes Nf1E4 ethyl methanesulfonate 0 Yes Nf1P1 loss of function allele Delta2-3 0 Yes Nf1P2 loss of function allele Delta2-3 0 Yes Nf1unspecified 0 --
Alleles Carried on Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nf1 Class Mutagen Stocks Known lesion Nf1GD13892 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nf1JF01767 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nf1JF01866 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Nf1dsRNA.cKa in vitro construct - RNAi 0 Yes Nf1dsRNA.si.cKa in vitro construct - RNAi 0 Yes Nf1GRD.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nf1hs.PT in vitro construct - regulatory fusion 0 Yes Nf1hs.PW in vitro construct - regulatory fusion 0 Yes Nf1K1468T.GRD.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nf1K1481A.GRD.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nf1K1481A.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nf1KK101909 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Nf1Q1471R.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nf1R1320A.GRD.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nf1R1320A.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nf1R1320P.GRD.Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Nf1R1320P.hs in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Nf1Scer\UAS.cBa in vitro construct - regulatory fusion 0 Yes Nf1Scer\UAS.cdNa in vitro construct - regulatory fusion 0 Yes Nf1Scer\UAS.cWa in vitro construct - regulatory fusion 0 Yes Nf1Δ1219-1580.hs in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Nf1Δ1611-1769.hs in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Nf1Δ1770-2265.hs in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Nf1Δ492-1094.hs in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)Espl3 (Williams et al., 2001) Df(3R)Nf1P1 (Cooper and Bray, 1999, Guo et al., 1997, The et al., 1997)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD13892} NA P{KK101909} NA P{TRiP.JF01767} NA P{TRiP.JF01866} NA P{UAS-Nf1.B} NA P{UAS-Nf1.dN} NA P{UAS-Nf1.GRD.K1468T} NA P{UAS-Nf1.GRD.K1481A} NA P{UAS-Nf1.GRD.R1320A} NA P{UAS-Nf1.GRD.R1320P} NA P{UAS-Nf1.GRD} NA P{UAS-Nf1.W} NA heat-shock construct P{hs-Nf1.2802} NA P{hs-Nf1.K1481A} NA P{hs-Nf1.Q1471R} NA P{hs-Nf1.R1320A} NA P{hs-Nf1.R1320P} NA P{hs-Nf1.Δ492-1094} NA P{hs-Nf1.Δ1219-1580} NA P{hs-Nf1.Δ1611-1769} NA P{hs-Nf1.Δ1770-2265} NA P{hsNF1} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}Nf1MI01388 NA PBac{PB}Nf1c00472 NA PBac{PB}Nf1c00617 NA PBac{PB}Nf1c02945 NA PBac{RB}Nf1e00084 NA insertion of enhancer trap P{lacW}Nf1P2 No
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
Ras GTPase activator activity inferred from direct assay (The et al., 1997)
Biological Process
CV term Evidence References
cAMP-mediated signaling inferred from mutant phenotype (The et al., 1997) determination of adult lifespan inferred from mutant phenotype (Tong et al., 2007) locomotor rhythm inferred from mutant phenotype (Williams et al., 2001) long-term memory inferred from mutant phenotype (Buchanan and Davis, 2010) olfactory learning inferred from mutant phenotype (Guo et al., 2000, Buchanan and Davis, 2010) perineurial glial growth inferred from genetic interaction with ine (Yager et al., 2001) inferred from genetic interaction with poe (Yager et al., 2001) positive regulation of adenylate cyclase activity inferred from mutant phenotype (Guo et al., 1997) positive regulation of multicellular organism growth inferred from mutant phenotype (Walker et al., 2006) Ras protein signal transduction inferred from genetic interaction with Ras85D (Williams et al., 2001) inferred from genetic interaction with Sos (Williams et al., 2001) regulation of multicellular organism growth inferred from mutant phenotype (The et al., 1997) regulation of Ras protein signal transduction inferred from mutant phenotype (Walker et al., 2006) response to heat inferred from mutant phenotype (Tong et al., 2007) response to oxidative stress inferred from mutant phenotype (Tong et al., 2007) short-term memory inferred from mutant phenotype (Guo et al., 2000, Buchanan and Davis, 2010)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
binding inferred from electronic annotation with InterPro:IPR016024 (FlyBase Curators et al., 2004-) Ras GTPase activator activity inferred from sequence or structural similarity with UniProtKB:P21359 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component
CV term Evidence References
intracellular inferred from electronic annotation with InterPro:IPR001936, InterPro:IPR008936 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nf1 allele Gene References Nf1P1 Ras85D (Williams et al., 2001) Sos (Williams et al., 2001) Nf1P2 eag (Yager et al., 2001) ine (Yager et al., 2001) poe (Yager et al., 2001) Ras85D (Williams et al., 2001) rl (Williams et al., 2001) Sos (Williams et al., 2001) Nf1unspecified dnc (Williams et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68049 DroID - A comprehensive database of gene and protein interactions. • FBgn0015269 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43149
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16794 Dere\GG11448 Dgri\GH19449 Dmoj\GI24684 Dper\GL21812 Dpse\GA26413 Dsim\GD21256 Dvir\GJ23901 Dwil\GK12879 Dyak\GE23642
Curated drosophilid orthologs	Dpse\GA20982
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL008371-PA Aedes aegypti (mosquito) AGAP003140-PA Anopheles gambiae (mosquito) XP_624747.2 Apis mellifera (honey bee) 34519 Batrachochytrium dendrobatidis (chytrid fungus) 95684 Branchiostoma floridae (lancelet) BGIBMGA011404-PA Bombyx mori (silk worm) ENSBTAP00000015699 Bos taurus (bovine) CAL0003508 Candida albicans (a form of yeast) ENSCAFP00000027461 Canis familiaris (dog) CAGL0M09999g Candida glabrata (haploid yeast) ENSCINP00000028345 Ciona intestinalis (sea squirt) cn06291 Cryptococcus neoformans (yeast-like fungus) CPIJ011774 Culex pipens (mosquito) ENSCPOP00000005513 Cavia porcellus (guinea pig) ENSCSAVP00000014529 Ciona savignyi (sedentary tunicate) 172215 Capitella spl (polychaete worm) 307021 Daphnia pulex (daphnia) ENSDARP00000004724 Danio rerio (zebra fish) ENSECAP00000007525 Equus caballus (horse) ENSGACP00000006585 Gymnopilus aculeatus (mushroom) ENSGALP00000009067 Gallus gallus (chicken) 117118 Hypsipyla robusta (cedar shoot borer) ENSP00000351015 Homo sapiens (human) KLLA0E17887g Kluyveromyces lactis (yeast) 104331 Lottia gigantea (owl limpet) ENSMODP00000023994 Monodelphis domestica (gray opossum) ENSMMUP00000011285 Macaca mulatta (rhesus monkey) ENSMUSP00000071289 Mus musculus (house mouse) 20131 Nematostella vectensis (starlet anemone) XP_001602698.1 Nasonia vitripennis (wasp) ENSORLP00000018929 Oryzias latipes (japanese killifish) PHUM002920-PA Pediculus humanus (human body louse) PP11921 Pristionchus pacificus (nematode) ENSPPYP00000009159 Pongo pygmaeus (orangutan) ENSPTRP00000015312 Pan troglodytes (chimpanzee) ENSRNOP00000049106 Rattus norvegicus (norway rat) YOL081W Saccharomyces cerevisiae (budding yeast) Smp_148460 Schistosoma mansoni (trematode parasite) XP_001196417.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001196949.1 Strongylocentrotus purpuratus (purple sea urchin) 59057 Trichoplax adhaerens (metazoa) ENSTNIP00000007654 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000014207 Takifugu rubripes (pufferfish Takifugu) YALI0B07535g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15474 LH25084 FBgn0240813 FBgn0214888 FBgn0210995 FBgn0192705 FBgn0186855 FBgn0165242 FBgn0165239 JGI_V11_2283 JGI_V11_307021 FBgn0247787 FBgn0159405 FBgn0147406 FBgn0126914 FBgn0103747 FBgn0093815 CPIJ011774 BGIBMGA011405 BGIBMGA011404 GB12613 AGAP003140 AAEL008371 PB21627 PHUM364110 GLEAN_04757 GLEAN_04756
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	10201 w1118; PBac{PB}Nf1c00617 25845 y1 v1; P{TRiP.JF01866}attP2 31466 y1 v1; P{TRiP.JF01767}attP2 34427 y1 w*; Mi{MIC}Nf1MI01388/TM3, Sb1 Ser1
Harvard	- PBac{PB}Nf1c00617 - PBac{PB}Nf1c02945 - PBac{RB}Nf1e00084
VDRC	v35877 w1118; P{GD13892}v35877/TM3
Genomic Clones ( 2 )
	BACR13F13 BACR22N13 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 36 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH08833 IP16186
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	12871 13223 17607 50604 107980 163964 166517 214567.4 CK01.29C.C4 CK01.54D.F8 CK01.70B.F3 CK01.90D.G3 CK01.95A.D2 CK01.99A.A6 CK01.101A.F5 EK044456 EK054317 EK056022 EK063607 EK071126 EK073934 EK085223 EK114619 EK115150 EK173417 EK187504 EK190145 EK201527 EK234017 EK268455 EK290116 EP11916 IP16386 RH34928
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015269 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43149
Antibody Information
	monoclonal (The et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Nf1 mutant flies are defective in memory acquisition but not memory stability. (Buchanan and Davis, 2010) One of 42 Drosophila genes identified as being most likely to reveal molecular and cellular mechanisms of nervous system development or plasticity relevant to human Mental Retardation disorders. (Inlow and Restifo, 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Nf1 has a role in circadian output via the Ras/MAPK signaling pathway. (Williams et al., 2001) Nf1 affects learning and short-term memory independently of its developmental effects. (Guo et al., 2000) Nf1 functions either upstream of adenylyl cyclase or in a parallel pathway. (Bernards, 1998.11.6) Nf1 deficient phenotypes (reduced size, absence of a PACAP-induced rectifying potassium current at the larval neuromuscular junction) are not modified by manipulating Ras85D or Ras64B gene dosage, but rather are rescued by increasing signaling through the cAMP - PKA pathway. (Bernards, 1998.11.6) Activation of rut protein through heterotrimeric guanine nucleotide-binding protein-coupled receptor is regulated by Nf1. (Guo et al., 1997) Nf1 and Pka-C1 interact in a pathway that controls the overall growth of flies. (The et al., 1997) Nf1 is a crucial component for activation of the cAMP pathway. (Zhong, 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_170252 NM_170253 RefSeq (Proteins) • NP_733131 NP_733132 NP_001014668 DDBJ / EMBL / GenBank • AAB58975 AAB58976 AAB58977 AAF56543 AAF56543.2 AAF56543.3 AAL13548 AAN14067 AAN14067.1 AAN14067.2 AAX52994 AI109596 AJ276314 AW941718 AY058319 BI563967 CZ466483 CZ466605 CZ468345 CZ477989 L26500 L26501 L26502 Entrez Gene - A searchable database of RefSeq genes. • 43149 UniProtKB/TrEMBL • O01397 O01398 O01399 Q8IMS2 Q9VBJ2 Q59DT9 Q95U43
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Cellular retinaldehyde-binding/triple function, C-terminal (IPR001251) Ras GTPase-activating protein (IPR001936) Rho GTPase activation protein (IPR008936) Armadillo-type fold (IPR016024)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68049 DroID - A comprehensive database of gene and protein interactions. • FBgn0015269 DRSC - Results from RNAi screens. • FBgn0015269 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015269 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8318-RB;CG8318-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015269 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43149 InParanoid A subset of ortholog calls from InParanoid. • AAEL008371-PA Aedes aegypti (mosquito) AGAP003140-PA Anopheles gambiae (mosquito) XP_624747.2 Apis mellifera (honey bee) 34519 Batrachochytrium dendrobatidis (chytrid fungus) 95684 Branchiostoma floridae (lancelet) BGIBMGA011404-PA Bombyx mori (silk worm) ENSBTAP00000015699 Bos taurus (bovine) CAL0003508 Candida albicans (a form of yeast) ENSCAFP00000027461 Canis familiaris (dog) CAGL0M09999g Candida glabrata (haploid yeast) ENSCINP00000028345 Ciona intestinalis (sea squirt) cn06291 Cryptococcus neoformans (yeast-like fungus) CPIJ011774 Culex pipens (mosquito) ENSCPOP00000005513 Cavia porcellus (guinea pig) ENSCSAVP00000014529 Ciona savignyi (sedentary tunicate) 172215 Capitella spl (polychaete worm) 307021 Daphnia pulex (daphnia) ENSDARP00000004724 Danio rerio (zebra fish) ENSECAP00000007525 Equus caballus (horse) ENSGACP00000006585 Gymnopilus aculeatus (mushroom) ENSGALP00000009067 Gallus gallus (chicken) 117118 Hypsipyla robusta (cedar shoot borer) ENSP00000351015 Homo sapiens (human) KLLA0E17887g Kluyveromyces lactis (yeast) 104331 Lottia gigantea (owl limpet) ENSMODP00000023994 Monodelphis domestica (gray opossum) ENSMMUP00000011285 Macaca mulatta (rhesus monkey) ENSMUSP00000071289 Mus musculus (house mouse) 20131 Nematostella vectensis (starlet anemone) XP_001602698.1 Nasonia vitripennis (wasp) ENSORLP00000018929 Oryzias latipes (japanese killifish) PHUM002920-PA Pediculus humanus (human body louse) PP11921 Pristionchus pacificus (nematode) ENSPPYP00000009159 Pongo pygmaeus (orangutan) ENSPTRP00000015312 Pan troglodytes (chimpanzee) ENSRNOP00000049106 Rattus norvegicus (norway rat) YOL081W Saccharomyces cerevisiae (budding yeast) Smp_148460 Schistosoma mansoni (trematode parasite) XP_001196417.1 Strongylocentrotus purpuratus (purple sea urchin) XP_001196949.1 Strongylocentrotus purpuratus (purple sea urchin) 59057 Trichoplax adhaerens (metazoa) ENSTNIP00000007654 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000014207 Takifugu rubripes (pufferfish Takifugu) YALI0B07535g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /neural/nerf1-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43149 modMine - Data generated by the modENCODE project. • FBgn0015269 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV15474 LH25084 FBgn0240813 FBgn0214888 FBgn0210995 FBgn0192705 FBgn0186855 FBgn0165242 FBgn0165239 JGI_V11_2283 JGI_V11_307021 FBgn0247787 FBgn0159405 FBgn0147406 FBgn0126914 FBgn0103747 FBgn0093815 CPIJ011774 BGIBMGA011405 BGIBMGA011404 GB12613 AGAP003140 AAEL008371 PB21627 PHUM364110 GLEAN_04757 GLEAN_04756
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	CG8318 (Kim et al., 2004, Perkins et al., 1.15.2009, Ni et al., 2008, Keleman et al., 2009.8.5) dNF1 (Holmes and Sheeba, 2005) NF1 (Vidal and Cagan, 2006, Bilder, 2004, Tong et al., 2003, Hashimoto et al., 2002, Zheng and Sehgal, 2001, Yager et al., 2001, Yager et al., 2001, Roman and Davis, 2001, Davis, 2001, Zhu and Parada, 2001, vanden Broeck, 2001, Stocker and Hafen, 2000, Yager et al., 2000, Brill et al., 2000, Guo et al., 2000, Potter et al., 2000, Bernards, 1997.4.8, Bernards, 1997.4.8, Bernards, 1997.4.8, Cooper and Bray, 1999, Brill et al., 1998, The et al., 1997, Guo et al., 1997, The et al., 1995, Letendre et al., 1993, Walker et al., 2006, Oldham et al., 2000, Tong et al., 2007, Slack et al., 2010, Liu et al., 2009, Hong et al., 2008) NF-1 (Hendricks, 2003, Mittnach, 2000) Nf-1 (Stanewsky, 2003) Nf1 (Lavery and Stern, 2007, Jordan et al., 2005, The et al., 1997, Williams et al., 2001, Stanewsky, 2003, Stanewsky, 2002, Sokolowski, 2001, Maines et al., 2007, Bakal et al., 2007, Tong et al., 2002, Mertens et al., 2005, Kankel et al., 2007, Gilchrist et al., 2008, Ho et al., 2007, Hannan et al., 2006, Gouzi et al., 2011) nf1 (Buchanan and Davis, 2010)
Name Synonym	Neurofibromatosis-1 (Stanewsky, 2002, Stanewsky, 2003, Williams et al., 2001) neurofibromatosis 1 (Walker et al., 2006) Neurofibromatosis 1 (Liu et al., 2009) Neurofibromatosis Factor 1 (Mertens et al., 2005) neurofibromatosis type 1 (Burrell and Sahley, 2001, Zars, 2000, Stocker and Hafen, 2000) Neurofibromin (Tong et al., 2002) neurofibromin (Buchanan and Davis, 2010) Neurofibromin 1 (Yager et al., 2000, Mummery-Widmer et al., 2009)
Secondary FlyBase IDs
References ( 87 )
Generate a list of	All references relating to this gene ( 87 )
List References by type	Research paper  ( 28 ) Supplementary material  ( 5 ) Review  ( 22 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 13 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 4 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 1 ) Letter  ( 1 )
Recent research papers ( 4 )
	Gouzi et al., 2011, PLoS Genet. 7(9): e1002281 The receptor tyrosine kinase alk controls neurofibromin functions in Drosophila growth and learning. [FBrf0216258] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Buchanan and Davis, 2010, J. Neurosci. 30(30): 10135--10143 A distinct set of Drosophila brain neurons required for neurofibromatosis type 1-dependent learning and memory. [FBrf0211411] Slack et al., 2010, PLoS Genet. 6(3): e1000881 Regulation of lifespan, metabolism, and stress responses by the Drosophila SH2B protein, Lnk. [FBrf0210418] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...