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Gene Dmel\Orc2

General Information
Symbol	Dmel\Orc2	Species	D. melanogaster
Name	Origin recognition complex subunit 2	Annotation symbol	CG3041
Feature type	protein_coding_gene	FlyBase ID	FBgn0015270
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	DmORC2, k43, l(3)k43, ORC, l(3)88Ab
Genomic Location
Chromosome (arm)	3R	Recombination map	3-51.1
Cytogenetic map	88A3-88A3	Sequence location	3R:9,790,639..9,792,803 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Origin recognition complex subunit 2 is referred to in FlyBase by the symbol Dmel\Orc2 (CG3041, FBgn0015270). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: chromatin binding. There is experimental evidence that it is involved in the biological process: mitotic chromosome condensation; DNA-dependent DNA replication initiation; cell proliferation; eggshell chorion gene amplification; mitotic spindle organization; DNA replication; chromosome condensation; neurogenesis. 28 alleles are reported. The phenotypes of these alleles are annotated with: chromosome, centromeric region; chorion; trichogen cell; mitotic cell cycle; follicle cell; spindle; nuclear chromosome; cell cycle; imaginal disc; mitotic metaphase. It has one annotated transcript and one annotated polypeptide. Protein features are: Origin recognition complex, subunit 2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1635760_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Orc2. Gene sequence location is 3R:9790639..9792803.
External Summaries
Phenotypic Description from the Red Book (Lindsley & Zimm 1992)
Gene/Allele symbols may differ from current usage	fs(3)293-19 Eggs of homozygous females defective in late-deposited outer layers of endochorion; endochorionic material deficient. Late-gene expression as measured by mRNA or protein levels defective; deficiency in the level of chorion-gene amplification in follicle cells. l(3)88Ab Homozygous larvae contain rudimentary imaginal discs, but disc primordia do not grow during larval development; testes and ovaries smaller than normal, and cell number in central nervous system reduced. Mutant gonads do not survive metamorphosis when implanted into wild-type larvae. Homozygous cuticular clones appear to develop normally, but abdominal clones reduced in frequency and size compared to control clones. Mutant larvae support growth of implanted wild-type discs. Normal gene product postulated to be required for cell proliferation; survival of somatic epidermal clones attributed to perdurance. Larval ganglia exhibit extremely low mitotic index; chromosomes irregularly condensed and chromosome breakage observed (Gatti and Baker, 1989, Genes Dev. 3: 438-53); salivary chromosomes appear normal. Homozygous females weakly fertile; produce eggs with grossly defective chorions; thin, missing most appendages and permeable. All chorion proteins reduced in abundance; chorion gene amplification reduced to 10% wild-type level (Snyder, Galanopoulos, and Kafatos, l986, Proc. Nat. Acad. Sci. USA 83: 3341-45). Heterozygotes with lethal alleles more extreme and sterile.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms alpha-heterochromatin chromatin binding heterochromatin Transgenic Constructs P{Orc2-GFP.C} P{Orc2-GFP.N} References Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Alleles Orc2[T:Avic\GFP.N] Orc2[T:Avic\GFP.C]
FB2012_01	References Vatolina et al., 2011, Genetika, Moscow 47(5): 597--609 Vatolina et al., 2011, Russ. J. Genet. 47(5): 521--532 Sequence features HFA16776 BKN22948 BKN45317
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88A3-88A3   Limits computationally determined from genome sequence between P{PZ}flfl01949 and P{lacW}trxj14A6&P{PZ}trx00347
Experimentally Determined Cytological Location
Cytogenetic map Notes References 88A3-88A5   (determined by in situ hybridisation)   (Chesnokov et al., 1999)
Experimentally Determined Recombination Data
Location	3-51.1 (Tearle and Nusslein-Volhard, 1987, Gatti and Baker, 1989, )
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Orc2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Orc2-RA FBtr0082866  NM_169563   1969   618
Additional Transcript Data & Comments
Reported size (kB)	2.5 (northern blot) (Landis et al., 1997) 2.2 (northern blot) (Chesnokov et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Orc2-PA   FBpp0082329   69.0   618   6.26     NP_731873   AAF55006
Additional Polypeptide Data & Comments
Reported size (kDa)	618 (aa); 82 (kD observed) (Gossen et al., 1995)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Origin recognition complex, subunit 2 (IPR007220)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA264739   AE014297 AAF55006   AF246305 AAF99606   AI135904   AI513274   AI530859   AI541705   AY051471 AAK92895   BF490714   BF492809   BG639942   BI640609   BI641790   GH943131   GH949588   GH956247   GH957921   GH963124   GH974331   GH982612   GH984827   U43504 AAC46955
UniProtKB/Swiss-Prot	Q24168
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage & larval stage     (Chesnokov et al., 1999)
Additional Descriptive Data
	Orc2 transcripts are maternally deposited and are abundant in early embryos. Levels taper off in later embryonic and larval stages. (Chesnokov et al., 1999)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference oogenesis stage S10B   egg chamber   • nucleus (Cayirlioglu et al., 2001) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 9     (Gossen et al., 1995) Comment:reference states 0-4 hr AEL
Additional Descriptive Data
	Western blot analysis shows that Orc2 protein is most abundant in 0-4hr embryos. (Gossen et al., 1995) Orc2 protein is preferentially localized to heterochromatin on interphase and mitotic chromosomes. (Pak et al., 1997)
Marker for
Subcellular Localization
CV Term	nucleus (Gossen et al., 1995) heterochromatin centric heterochromatin alpha-heterochromatin (Pak et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0015270    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0015270 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0015270 expression level Developmental Stage   Expression Level embryo 00-02hr    3357 embryo 02-04hr    976 embryo 04-06hr    2535 embryo 06-08hr    1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day    1778 adult female 30day    1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3357) embryo 02-04hr  (976) embryo 04-06hr  (2535) embryo 06-08hr  (1804) embryo 08-10hr  (1127) embryo 10-12hr  (527) embryo 12-14hr  (259) embryo 14-16hr  (156) embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1  (199) larva L2  (208) larva L3 12hr old  (141) larva L3 puffstage 1-2  (224) larva L3 puffstage 3-6  (274) larva L3 puffstage 7-9  (275) white prepupae new  (197) white prepupae 12hr  (435) white prepupae 24hr  (289) pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day  (688) adult female 05day  (1778) adult female 30day  (1864) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3357) embryo 02-04hr    976 embryo 04-06hr  (2535) embryo 06-08hr  (1804) embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day  (1778) adult female 30day  (1864) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3357 embryo 02-04hr    976 embryo 04-06hr    2535 embryo 06-08hr    1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day    1778 adult female 30day    1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3357 embryo 02-04hr    976 embryo 04-06hr    2535 embryo 06-08hr    1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day    1778 adult female 30day    1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0015270 expression level Developmental Stage   Expression Level embryo 00-02hr    3357 embryo 02-04hr    976 embryo 04-06hr    2535 embryo 06-08hr    1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day    1778 adult female 30day    1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (3357) embryo 02-04hr  (976) embryo 04-06hr  (2535) embryo 06-08hr  (1804) embryo 08-10hr  (1127) embryo 10-12hr  (527) embryo 12-14hr  (259) embryo 14-16hr  (156) embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1  (199) larva L2  (208) larva L3 12hr old  141 larva L3 puffstage 1-2  (224) larva L3 puffstage 3-6  (274) larva L3 puffstage 7-9  (275) white prepupae new  (197) white prepupae 12hr  (435) white prepupae 24hr  (289) pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day  (688) adult female 05day  (1778) adult female 30day  (1864) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (3357) embryo 02-04hr    976 embryo 04-06hr  (2535) embryo 06-08hr  1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day  1778 adult female 30day  1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    3357 embryo 02-04hr    976 embryo 04-06hr    2535 embryo 06-08hr    1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day    1778 adult female 30day    1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    3357 embryo 02-04hr    976 embryo 04-06hr    2535 embryo 06-08hr    1804 embryo 08-10hr    1127 embryo 10-12hr    527 embryo 12-14hr    259 embryo 14-16hr    156 embryo 16-18hr    79 embryo 18-20hr    62 embryo 20-22hr    59 embryo 22-24hr    59 larva L1    199 larva L2    208 larva L3 12hr old    141 larva L3 puffstage 1-2    224 larva L3 puffstage 3-6    274 larva L3 puffstage 7-9    275 white prepupae new    197 white prepupae 12hr    435 white prepupae 24hr    289 pupae 2d postWPP    75 pupae 3d postWPP    47 pupae 4d postWPP    83 adult male 01day    59 adult male 05day    91 adult male 30day    97 adult female 01day    688 adult female 05day    1778 adult female 30day    1864 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0015270    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0015270 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0015270 expression level Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0015270 expression level Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    86.3 Larval Midgut    13.7 Larval Hindgut    18.4 Larval Malpighian Tubules    11.6 Larval Fat Body no informative data Larval Salivary Gland    33.4 Larval Trachea    30.1 Larval Carcass    17.1 Adult Head    6.1 Adult Eye    3.85 Adult Brain    5 Adult Thoracic-Abdominal Ganglion    8.8 Adult Crop no informative data Adult Midgut    17 Adult Hindgut    6.6 Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland    9.3 Adult Heart    2.95 Adult VirginFemale Spermatheca    6.2 Adult InseminatedFemale Spermatheca no informative data Adult Ovary    228 Adult Testis    15.5 Adult Male Accessory Gland no informative data Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut     Adult Malpighian Tubules no informative data Adult Fat Body no informative data Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca no informative data Adult Ovary     Adult Testis     Adult Male Accessory Gland no informative data Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    6.1   NA  Eye    3.85   NA  Brain    5   86.3  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    8.8   NA  Crop    no informative data   13.7  Midgut    17   18.4  Hindgut    6.6   11.6  Malpighian Tubules    no informative data   no informative data  Fat Body    no informative data   33.4  Salivary Gland    9.3   NA  Heart    2.95   30.1  Trachea    NA   NA  VirginFemale Spermatheca    6.2   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    228   NA  Testis    15.5   NA  Male Accessory Gland    no informative data   17.1  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015270 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3041-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Orc2 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Orc21 Orc2CB03773 Orc2γ4 lethal, with Scer\GAL41407 Orc2GD4122 Orc2KK102852 lethal (with Orc21) Orc2γ4 lethal (with Orc2γ4) Orc21 lethal | larval stage | recessive Orc21 lethal | pupal stage | recessive Orc2unspecified lethal | recessive Orc21 Orc2M10 Orc2M27 Orc2Z33 Orc2Z47 Orc2γ3 Orc2γ4 lethal | recessive | cold sensitive Orc2ZWD viable Orc22 viable, with Scer\GAL4pnr-MD237 Orc2GD4122 Sterility Allele female sterile Orc22 female sterile | recessive Orc22 Other Phenotypes Allele mitotic cell cycle defective Orc21 neuroanatomy defective | larval stage, with Scer\GAL41407 Orc2GD4122 suppressor of variegation, with In(1)wm4h Orc22 suppressor of variegation | dominant, with In(1)wm4h Orc21 Orc2γ4 visible, with Scer\GAL4pnr-MD237 Orc2GD4122 wild-type Orc2+t7.5 Orc2+t10.5 Phenotype manifest in Allele cell cycle Orc21 chorion Orc22 chromatin | larval stage Orc2Z33 chromosome, centromeric region Orc2unspecified embryonic/larval neuroblast | larval stage, with Scer\GAL41407 Orc2GD4122 follicle cell Orc2γ3 ganglion mother cell | larval stage, with Scer\GAL41407 Orc2GD4122 imaginal disc Orc21 Orc2unspecified mitosis & nuclear chromosome Orc2γ4 Orc21 Orc2unspecified mitosis & nuclear chromosome (with Df(3R)293γ7) Orc21 mitosis & nuclear chromosome (with Orc21) Orc2γ4 mitosis & nuclear chromosome (with Orc2γ4) Orc21 mitotic cell cycle Orc2γ4 Orc21 Orc2unspecified mitotic metaphase Orc21 mitotic metaphase (with Df(3R)293γ7) Orc21 mitotic metaphase (with Orc21) Orc2γ4 mitotic metaphase (with Orc2γ4) Orc21 nuclear chromosome Orc2dsRNA.cCa nuclear chromosome | larval stage Orc2Z33 polytene chromosome | larval stage Orc2γ4 spindle Orc21 trichogen cell, with Scer\GAL4pnr-MD237 Orc2GD4122
Classical Alleles ( 19 )
For All Classical Alleles Show
Allele of Orc2 Class Mutagen Stocks Known lesion Orc21 ethyl methanesulfonate 4 Yes Orc2γ4 gamma ray 3 Yes Orc2EY04752 P-element activity 1 -- Orc22 ethyl methanesulfonate 0 Yes Orc23 gamma ray 0 -- Orc24 gamma ray 0 -- Orc25 gamma ray 0 -- Orc26 gamma ray 0 -- Orc27 ethyl methanesulfonate 0 -- Orc28 ethyl methanesulfonate 0 -- Orc2b51 ethyl methanesulfonate 0 -- Orc2CB03773 P-element activity 0 Yes Orc2M10 ethyl methanesulfonate 0 -- Orc2M27 ethyl methanesulfonate 0 -- Orc2unspecified 0 -- Orc2Z33 ethyl methanesulfonate 0 -- Orc2Z47 ethyl methanesulfonate 0 -- Orc2ZWD ethyl methanesulfonate 0 -- Orc2γ3 gamma ray 0 --
Alleles Carried on Transgenic Constructs ( 9 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Orc2 Class Mutagen Stocks Known lesion Orc2GD4122 in vitro construct - RNAi in vitro construct - regulatory fusion 4 Yes Orc2KK102852 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Orc2+t10.5 in vitro construct - genomic fragment 0 Yes Orc2+t7.5 in vitro construct - genomic fragment 0 Yes Orc2BB7.5 in vitro construct - genomic fragment 0 Yes Orc2dsRNA.cCa in vitro construct - RNAi 0 Yes Orc2MtnA.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Orc2T:Avic\GFP.C in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Orc2T:Avic\GFP.N in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)293γ5 (Jarman and Collett, 1998, Mortin et al., 1992, Landis et al., 1997) Df(3R)293γ7 (Jarman and Collett, 1998, Landis et al., 1997) Df(3R)Exel6171 (Ryder, 2004.4.26) Df(3R)Exel6288 (Ryder, 2004.4.26) Df(3R)red3l (Jarman and Collett, 1998) Df(3R)red-P52 (Jarman and Collett, 1998, Mortin et al., 1992, Landis et al., 1997)
Not disrupted in	Df(3R)l26c (Jarman and Collett, 1998) Df(3R)M36 (Jarman and Collett, 1998) Df(3R)M49 (Jarman and Collett, 1998, Mortin et al., 1992) Df(3R)red-P1 (Jarman and Collett, 1998, Mortin et al., 1992) Df(3R)red-P6 (Mortin et al., 1992) Df(3R)red-P93 (Mortin et al., 1992, Landis et al., 1997) Df(3R)su(Hw)7 (Jarman and Collett, 1998) Df(3R)su(Hw)V (Mortin et al., 1992) Df(3R)urd (Jarman and Collett, 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4122} NA P{KK102852} NA characterization construct P{BB7.5} NA P{BX10.5} NA P{HX7.5} NA P{Orc2-GFP.C} NA P{Orc2-GFP.N} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}Orc2EY04752 NA miscellaneous insertions P{PTT-GB}CG9925CB04400 NA P{PTT-GB}Orc2CB03773 NA
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 13 terms )
Molecular Function
CV term Evidence References
chromatin binding inferred from direct assay (Baldinger and Gossen, 2009)
Biological Process
CV term Evidence References
cell proliferation inferred from mutant phenotype (Park and Asano, 2008) chromosome condensation inferred from mutant phenotype (Christensen and Tye, 2003) DNA-dependent DNA replication initiation inferred from direct assay (Chesnokov et al., 1999) DNA replication inferred from mutant phenotype (Loupart et al., 2000, Pflumm and Botchan, 2001, Christensen and Tye, 2003) eggshell chorion gene amplification inferred from mutant phenotype (Zhang and Tower, 2004) mitotic chromosome condensation inferred from mutant phenotype (Pflumm and Botchan, 2001) mitotic spindle organization inferred from mutant phenotype (Pflumm and Botchan, 2001) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component
CV term Evidence References
alpha-heterochromatin inferred from direct assay (Pak et al., 1997) heterochromatin inferred from direct assay (Pak et al., 1997) nuclear origin of replication recognition complex inferred from direct assay (Chesnokov et al., 1999) nucleus inferred from direct assay (Gossen et al., 1995)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Biological Process
CV term Evidence References
chromatin silencing non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) DNA-dependent DNA replication inferred from sequence or structural similarity with Saccharomyces Orc2 (Gossen et al., 1995) non-traceable author statement (Pak, 1995.12.17, Swiss-Prot Project Members, 1997.2.1) DNA replication inferred from sequence or structural similarity with mouse Orc2 (FlyBase, 1992-) traceable author statement (Lee and Orr-Weaver, 2003) eggshell chorion gene amplification traceable author statement (Pflumm and Botchan, 2001, Lee and Orr-Weaver, 2003)
Cellular Component
CV term Evidence References
nuclear origin of replication recognition complex inferred from sequence or structural similarity with mouse Orc2 (FlyBase, 1992-) inferred from sequence or structural similarity with Saccharomyces Orc2 (Gossen et al., 1995) non-traceable author statement (Pak, 1995.12.17, Swiss-Prot Project Members, 1997.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Orc2 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 66780 DroID - A comprehensive database of gene and protein interactions. • FBgn0015270 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41703
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16229 Dere\GG16829 Dgri\GH22255 Dmoj\GI21981 Dper\GL24230 Dpse\GA15825 Dsec\GM24145 Dsim\GD18940 Dvir\GJ14342 Dwil\GK22682 Dyak\GE24212
Curated drosophilid orthologs	Dmoj\Orc2 (Calvi et al., 2007) Dpse\GA15825   Dsec\Orc2 (Calvi et al., 2007) Dsim\Orc2 (Calvi et al., 2007)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007457-PA Aedes aegypti (mosquito) AGAP000474-PA Anopheles gambiae (mosquito) 21626 Batrachochytrium dendrobatidis (chytrid fungus) 130922 Branchiostoma floridae (lancelet) XP_001894782.1 Brugia malayi (roundworm nematode) BGIBMGA012496-PA Bombyx mori (silk worm) ENSBTAP00000042074 Bos taurus (bovine) CAL0004335 Candida albicans (a form of yeast) CN18715 Caenorhabditis brenneri (small nematode) CBP21204 Caenorhabditis briggsae (nematode) CE11492 Caenorhabditis elegans (worm) ENSCAFP00000017767 Canis familiaris (dog) CAGL0H10230g Candida glabrata (haploid yeast) JA14062 Caenorhabditis japonica (nematode) cn11225 Cryptococcus neoformans (yeast-like fungus) CPIJ006319 Culex pipens (mosquito) ENSCPOP00000004102 Cavia porcellus (guinea pig) RP29233 Caenorhabditis remanei (nematode) 126602 Capitella spl (polychaete worm) DEHA2F06578g Debaryomyces Hansenii (yeast) 307817 Daphnia pulex (daphnia) ENSECAP00000007620 Equus caballus (horse) ENSGACP00000016527 Gymnopilus aculeatus (mushroom) ENSGALP00000013386 Gallus gallus (chicken) ENSP00000234296 Homo sapiens (human) ISCW019201-PA Ixodes scapularis (deer tick) KLLA0F18590g Kluyveromyces lactis (yeast) 161593 Lottia gigantea (owl limpet) ENSMODP00000018838 Monodelphis domestica (gray opossum) ENSMMUP00000018985 Macaca mulatta (rhesus monkey) ENSMUSP00000027198 Mus musculus (house mouse) 100805 Nematostella vectensis (starlet anemone) XP_001604000.1 Nasonia vitripennis (wasp) ENSOANP00000016003 Ornithorynchus anatinus (platypus) ENSORLP00000014430 Oryzias latipes (japanese killifish) LOC_Os03g08640.1 Oryza sativa (asian rice) MAL7P1.21 Plasmodium falciparum (protozoal parasite) PHUM006576-PA Pediculus humanus (human body louse) PP23365 Pristionchus pacificus (nematode) ENSPPYP00000014596 Pongo pygmaeus (orangutan) 144268 Phytophthora sojae (root rot pathogen) 414285 Populus trichocarpa (black cottonwood) ENSPTRP00000021863 Pan troglodytes (chimpanzee) ENSRNOP00000042867 Rattus norvegicus (norway rat) 4963811 Sorghum bicolor (sorghum) YBR060C Saccharomyces cerevisiae (budding yeast) SPBC685.09 Schizosaccharomyces pombe (fission yeast) XP_001193888.1 Strongylocentrotus purpuratus (purple sea urchin) 61999 Trichoplax adhaerens (metazoa) TA19765 Theileria annulata (apicomplexan parasite) XP_966877.2 Tribolium castaneum (red flour beetle) ENSTNIP00000010732 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008900 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000008981 Xenopus tropicalis (frog) YALI0D22330g Yarrowia lipolytica (nonconventional yeast) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB12061 NV15498 LH12080 ISCW019201 FBgn0241343 FBgn0224659 FBgn0201555 FBgn0190451 FBgn0179009 JGI_V11_307817 FBgn0075842 FBgn0161820 FBgn0144710 FBgn0129715 FBgn0109059 FBgn0093251 CPIJ006319 BGIBMGA012496 ACYPI45255 GB19049 AGAP000474 AAEL007457 PHUM085570 GLEAN_00631
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	9014 mwh1 Orc21 e1/TM1 15940 y1 w67c23; P{EPgy2}Orc2EY04752
Kyoto	109610 Orc21 / TM6B 109609 Orc2γ4 / TM6B 109608 y* w*; P{Orc2-GFP.N}MG54; Orc2γ4 / Orc21 109607 y* w*; P{Orc2-GFP.C}MG13 / CyO; Orc2γ4 / Orc21
VDRC	v107035 P{KK102852}VIE-260B v10388 w1118 P{GD4122}v10388 v47602 w1118; P{GD4122}v47602 v47603 w1118; P{GD4122}v47603 v47604 w1118; P{GD4122}v47604
Genomic Clones ( 1 )
	BACR25B05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 17 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH13824
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT01219 AT27274 LD08402 SD01605 SD06737 SD23541 SD25414
Other clones	RP004047741 RP004082795 RP004179936 RP004219080 RP004253408 RP004366086 RP004517872 RP004553429 SD09015
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015270 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41703
Antibody Information
	polyclonal (Gossen et al., 1995, Chesnokov et al., 1999)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: l(3)dsl5 l(3)SG44 (Shearn, 1995.5.3)
Additional comments
Other Comments
	The ATP dependent DNA binding activity of the Orc1/Orc2 protein complex is reduced or inhibited by phosphorylation of Orc2. (Remus et al., 2005) Mutants arrest in metaphase with abnormally condensed chromosomes that fail to align at the metaphase plate within a poorly assembled mitotic spindle. (Pflumm and Botchan, 2001) Orc2 functions in chromosome architecture and replication timing. (Loupart et al., 2000) The abundance of Orc2 is developmentally regulated and is greatest during the early stages of embryogenesis, concomitant with the highest rate of DNA replication. Fractionation of embryo nuclear extracts reveals that Orc2 is found in a tightly associated complex with five additional polypeptides. (Gossen et al., 1995) Homozygous larvae contain rudimentary imaginal discs, but disc primordia do not grow during larval development; testes and ovaries smaller than normal and cell number in central nervous system reduced. Mutant gonads do not survive metamorphosis when implanted into wild-type larvae. Homozygous cuticular clones appear to develop normally, but abdominal clones reduced in frequency and size compared to control clones. Mutant larvae support growth of implanted wild-type discs. Normal gene product postulated to be required for cell proliferation; survival of somatic epidermal clones attributed to perdurance. Larval ganglia exhibit extremely low mitotic index; chromosomes irregularly condensed and chromosome breakage observed (Gatti and Baker, 1989); salivary chromosomes appear normal.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169563 RefSeq (Proteins) • NP_731873 DDBJ / EMBL / GenBank • AAC46955 AAF55006 AAF99606 AAK92895 AF246305 AI135904 AI513274 AY051471 U43504 Entrez Gene - A searchable database of RefSeq genes. • 41703 UniProtKB/Swiss-Prot • Q24168
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Origin recognition complex, subunit 2 (IPR007220)
Linkouts
	BioGRID - A database of protein and genetic interactions • 66780 DroID - A comprehensive database of gene and protein interactions. • FBgn0015270 DRSC - Results from RNAi screens. • FBgn0015270 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015270 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3041-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015270 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 41703 InParanoid A subset of ortholog calls from InParanoid. • AAEL007457-PA Aedes aegypti (mosquito) AGAP000474-PA Anopheles gambiae (mosquito) 21626 Batrachochytrium dendrobatidis (chytrid fungus) 130922 Branchiostoma floridae (lancelet) XP_001894782.1 Brugia malayi (roundworm nematode) BGIBMGA012496-PA Bombyx mori (silk worm) ENSBTAP00000042074 Bos taurus (bovine) CAL0004335 Candida albicans (a form of yeast) CN18715 Caenorhabditis brenneri (small nematode) CBP21204 Caenorhabditis briggsae (nematode) CE11492 Caenorhabditis elegans (worm) ENSCAFP00000017767 Canis familiaris (dog) CAGL0H10230g Candida glabrata (haploid yeast) JA14062 Caenorhabditis japonica (nematode) cn11225 Cryptococcus neoformans (yeast-like fungus) CPIJ006319 Culex pipens (mosquito) ENSCPOP00000004102 Cavia porcellus (guinea pig) RP29233 Caenorhabditis remanei (nematode) 126602 Capitella spl (polychaete worm) DEHA2F06578g Debaryomyces Hansenii (yeast) 307817 Daphnia pulex (daphnia) ENSECAP00000007620 Equus caballus (horse) ENSGACP00000016527 Gymnopilus aculeatus (mushroom) ENSGALP00000013386 Gallus gallus (chicken) ENSP00000234296 Homo sapiens (human) ISCW019201-PA Ixodes scapularis (deer tick) KLLA0F18590g Kluyveromyces lactis (yeast) 161593 Lottia gigantea (owl limpet) ENSMODP00000018838 Monodelphis domestica (gray opossum) ENSMMUP00000018985 Macaca mulatta (rhesus monkey) ENSMUSP00000027198 Mus musculus (house mouse) 100805 Nematostella vectensis (starlet anemone) XP_001604000.1 Nasonia vitripennis (wasp) ENSOANP00000016003 Ornithorynchus anatinus (platypus) ENSORLP00000014430 Oryzias latipes (japanese killifish) LOC_Os03g08640.1 Oryza sativa (asian rice) MAL7P1.21 Plasmodium falciparum (protozoal parasite) PHUM006576-PA Pediculus humanus (human body louse) PP23365 Pristionchus pacificus (nematode) ENSPPYP00000014596 Pongo pygmaeus (orangutan) 144268 Phytophthora sojae (root rot pathogen) 414285 Populus trichocarpa (black cottonwood) ENSPTRP00000021863 Pan troglodytes (chimpanzee) ENSRNOP00000042867 Rattus norvegicus (norway rat) 4963811 Sorghum bicolor (sorghum) YBR060C Saccharomyces cerevisiae (budding yeast) SPBC685.09 Schizosaccharomyces pombe (fission yeast) XP_001193888.1 Strongylocentrotus purpuratus (purple sea urchin) 61999 Trichoplax adhaerens (metazoa) TA19765 Theileria annulata (apicomplexan parasite) XP_966877.2 Tribolium castaneum (red flour beetle) ENSTNIP00000010732 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000008900 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000008981 Xenopus tropicalis (frog) YALI0D22330g Yarrowia lipolytica (nonconventional yeast) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/orc2.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 41703 modMine - Data generated by the modENCODE project. • FBgn0015270 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB12061 NV15498 LH12080 ISCW019201 FBgn0241343 FBgn0224659 FBgn0201555 FBgn0190451 FBgn0179009 JGI_V11_307817 FBgn0075842 FBgn0161820 FBgn0144710 FBgn0129715 FBgn0109059 FBgn0093251 CPIJ006319 BGIBMGA012496 ACYPI45255 GB19049 AGAP000474 AAEL007457 PHUM085570 GLEAN_00631
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	CG3041 (Keleman et al., 2009.8.5) DmORC2 (Bosco et al., 2001, Loupart et al., 2000, DiAntonio, 1999, Chesnokov et al., 1999, Huang et al., 1998, Cavalli and Paro, 1998, Pak et al., 1997, Gossen et al., 1996, Gossen et al., 1995) DmORC 2 (Shareef et al., 2001) DmOrc2 (Baldinger and Gossen, 2009) DmOrc2p (Remus et al., 2005) fs(3)293 (Orr-Weaver, 1991) fs(3)293-19   k43 (Zhang and Tower, 2004, Tower, 2004, Spradling, 1999, Cryderman et al., 1999, Wallrath, 1998, Calvi et al., 1998, Landis et al., 1997, Baldinger and Gossen, 2009) l(3)88Ab (Jarman and Collett, 1998, Tearle and Nusslein-Volhard, 1987, ) l(3)A5   l(3)dsl5   l(3)k43 (Krause and Heck, 1996, Heck and Krause, 1997, Ripoll et al., 1987) l(3)K43 (Mortin et al., 1992, Gatti and Baker, 1989, Castellanos et al., 2008) l(3)SG44   ORC (Bielinsky et al., 2001, Wakimoto, 1998, Huang and Kellum, 1997, Eaton et al., 2010.12.17) ORC2 (Schwaiger, 2006, Sun and Deng, 2005, Craig, 2005, Aggarwal and Calvi, 2004, Manak et al., 2003, Loupart and Heck, 2001, Whittaker et al., 2000, Eissenberg and Elgin, 2000, Pak, 1995.12.17, Krause et al., 1999, Asano and Wharton, 1999, Pflumm and Botchan, 1999, DePamphilis, 1999, Wallrath, 1998, Csink and Henikoff, 1998, Anonymous, 1998, Kohzaki and Murakami, 2005, Badugu et al., 2005, Sun et al., 2008, Li et al., 2002, Sun et al., 2008, Park et al., 2007, Shareef et al., 2003, Macalpine et al., 2010, Deal et al., 2010, Lemos et al., 2010, Apger et al., 2010, Vatolina et al., 2011, Vatolina et al., 2011) orc2 (Claycomb and Orr-Weaver, 2005, Baldinger and Gossen, 2003, Lee and Orr-Weaver, 2003, Cenci et al., 2003, Loebel et al., 2000, Whittaker et al., 2000, Royzman et al., 1999, Royzman and Orr-Weaver, 1998, Foglietti et al., 2006, Hartl et al., 2007, Hyun et al., 2005, Park and Asano, 2008, Xie and Orr-Weaver, 2008) Orc2 (Wallace and Orr-Weaver, 2005, Buszczak et al., 2007, Cayirlioglu et al., 2003, Beall et al., 2007, Calvi et al., 2007, Ahlander et al., 2008, May et al., 2005, Baldinger and Gossen, 2009, Lin et al., 2009, Eaton et al., 2010.12.17, Gallone et al., 2011, Neumüller et al., 2011) Orc-2 (Somma et al., 2008) Orc2p (Ehrenhofer-Murray et al., 1995) rDmORC (Remus et al., 2004)
Name Synonym	lethal(3)88Ab   origin recognition complex (Lin et al., 2009) Origin recognition complex subunit 2
Secondary FlyBase IDs
	FBgn0001009 FBgn0002365
References ( 141 )
Generate a list of	All references relating to this gene ( 141 )
List References by type	Research paper  ( 71 ) Supplementary material  ( 4 ) Review  ( 28 ) Erratum  ( 1 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 21 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 ) Book  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 8 )
	Gallone et al., 2011, BMC Bioinformatics 12: 289 Bio::Homology::InterologWalk - A Perl module to build putative protein-protein interaction networks through interolog mapping. [FBrf0214774] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Vatolina et al., 2011, Genetika, Moscow 47(5): 597--609 [Identification and molecular genetic characterization of the polytene chromosome interbands in Drosophila melanogaster]. [FBrf0214456] Vatolina et al., 2011, Russ. J. Genet. 47(5): 521--532 Identification and molecular genetic characterization of the polytene chromosome interbands in Drosophila melanogaster. [FBrf0216692] Apger et al., 2010, Genetics 185(4): 1151--1165 Multiple functions for Drosophila mcm10 suggested through analysis of two mcm10 mutant alleles. [FBrf0211525] Deal et al., 2010, Science 328(5982): 1161--1164 Genome-wide kinetics of nucleosome turnover determined by metabolic labeling of histones. [FBrf0210910] Lemos et al., 2010, Proc. Natl. Acad. Sci. U.S.A. 107(36): 15826--15831 Epigenetic effects of polymorphic Y chromosomes modulate chromatin components, immune response, and sexual conflict. [FBrf0211775] Macalpine et al., 2010, Genome Res. 20(2): 201--211 Drosophila ORC localizes to open chromatin and marks sites of cohesin complex loading. [FBrf0209851] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...