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Gene Dmel\Rala

General Information
Symbol	Dmel\Rala	Species	D. melanogaster
Name	Ras-like protein A	Annotation symbol	CG2849
Feature type	protein_coding_gene	FlyBase ID	FBgn0015286
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	ral, dRal, DRala
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	3E5-3E6	Sequence location	X:3,599,017..3,613,665 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Ras-like protein A is referred to in FlyBase by the symbol Dmel\Rala (CG2849, FBgn0015286). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; PDZ domain binding; GTPase activity. There is experimental evidence that it is involved in the biological process: Ras protein signal transduction; innate immune response; GTP catabolic process; regulation of cell morphogenesis; border follicle cell migration; R3/R4 cell fate commitment; regulation of Notch signaling pathway; negative regulation of JNK cascade. 37 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; organ system; adult segment; external compound sense organ; sense organ; thoracic segment; adult; eo support cell; humeral trabecule; peripheral nervous system. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Small GTP-binding protein domain; Small GTPase superfamily; Small GTPase superfamily, Ras type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, larval/adult fat body, adult female reproductive system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640377_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Rala. Gene sequence location is X:3599017..3613665.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms protein binding Ras protein signal transduction
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 3E5-3E6   Limits computationally determined from genome sequence between P{EP}EP1623 and P{EP}EP911&P{EP}EP1094
Experimentally Determined Cytological Location
Cytogenetic map Notes References 3E-3E   (determined by in situ hybridisation) 3E1--4 (determined by in situ hybridisation) 3E5--F2 (determined by in situ hybridisation) 3E5--8 (determined by in situ hybridisation)   (Peter et al., 2002) 3E5-3F2   (determined by in situ hybridisation)   (Schaefer et al., 1999.12.2) 3E-3E   (determined by in situ hybridisation)   (Sawamoto et al., 1999) 3E1-3E4   (determined by in situ hybridisation)   (Schaefer et al., 1999.8.12) 3E-3E   (determined by in situ hybridisation)   (Schaefer et al., 1999.6.15) 3E-3E     (Camonis, 1999.4.6)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rala for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Due to significant sequence change in this region between Releases 4 and 5, data that predate Release 5 are not shown for some categories of evidence.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rala-RA FBtr0070583  NM_166986   2374   201 Rala-RB FBtr0070582  NM_166985   2054   197 Rala-RC FBtr0070584  NM_080324   2178   201
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rala-PA   FBpp0070558   22.9   201   7.82     NP_726882   AAF45889 Rala-PB   FBpp0070557   22.4   197   5.42     NP_726881   AAN09102 Rala-PC   FBpp0070559   22.9   201   7.82     NP_525063   AAF45890
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Small GTPase superfamily (IPR001806) Small GTP-binding protein domain (IPR005225) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Small GTPase superfamily, Ras type (IPR020849)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA433231   AA440572   AA539922   AA735651   AA802545   AA942199   AE014298 AAF45889   AE014298 AAF45890   AE014298 AAN09102   AI113973   AI135929   AI455190   AI530894   AJ300091   AJ300092   AJ300094   AY024366 AAK00587   AY118912 AAM50772   BH840647   BI166820   BI168674   BI169243   BI169360   BI169570   BI170453   BI172238   BI172902   BI173189   BI173387   BI173501   BI173758   BI173899   BI213695   BI214029   BI227703   BI230619   BI235175   BI237796   BI240431   BI242069   BI243247   BI361630   BI361742   BI362278   BI362349   BI363196   BI364013   BI364089   BI364122   BI364571   BI366952   BI369118   BI373217   BI373966   BI374246   BI374253   BI375427   BI483533   BI486517   BI578112   BI580163   BI580395   BI592319   BI608917   BI609428   BP551984   BP558347   BP558348   CO153341   CO154359   CO156943   CO177627   CO177930   CO178145   CO178236   CO181532   CO190391   CO190393   CO194307   CO263014   CO263376   CO264885   CO270427   CO272607   CO272623   CO272789   CO276257   CO277243   CO277480   CO279058   CO279064   CO279569   CO281675   CO282082   CO282383   CO286713   CO288198   CO291827   CO294022   CO296491   CO305197   CO307119   CO307705   CO308141   CO314218   CO316233   CO317439   CO318459   CO319781   CO321332   CO323830   CO327097   CO330503   CO333651   CO333792   CO338753   CO339452   CO344715   CO344795   CZ476521   CZ487923   CZ488703   CZ490658   DN154211   EC060249   EC063246   EC065028   EC066820   EC067082   EC069200   EC070779   EC072433   EC074493   EC075718   EC076773   EC077231   EC078957   EC081536   EC083310   EC083324   EC084909   EC086498   EC086860   EC197523   EC197762   EC202836   EC208227   EC208947   EC210867   EC211852   EC215910   EC216489   EC216656   EC216796   EC218550   EC219546   EC221584   EC224032   EC226403   EC226878   EC227974   EC228094   EC230087   EC230213   EC231603   EC233226   EC235402   EC236788   EC238108   EC238177   EC239090   EC239883   EC241353   EC241368   EC242623   EC242845   EC243639   EC252631   EC261899   EC264428   EC266722   ES688515   ES693322   ES693356   ES693803   ES693868   EV588394   EV598823   GH943035   GH943824   GH946253   GH949526   GH953024   GH953430   GH956300   GH956775   GH962524   GH963819   GH966783   GH968810   GH975455   GH977932   GH978326   GH979544   GH980328   GH980815   GH981035   GH982558   GH982898   GH982968   GH984149   U23800 AAC34902   Z31773
UniProtKB/Swiss-Prot	P48555
UniProtKB/TrEMBL	Q962I2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 4 -- 17   organism | ubiquitous   (Sawamoto et al., 1999) Comment:expressed in almost all cells third instar larval stage   embryonic/larval brain   (Sawamoto et al., 1999) ventral nerve cord | faint   (Sawamoto et al., 1999) wing disc   (Sawamoto et al., 1999) ventral thoracic disc   (Sawamoto et al., 1999) eye-antennal disc   (Sawamoto et al., 1999) oogenesis stage S9   egg chamber   (Ghiglione et al., 2008) border follicle cell   (Ghiglione et al., 2008) polar follicle cell   (Ghiglione et al., 2008)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Ghiglione et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, larval/adult fat body, adult female reproductive system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    587.075 Larval Midgut    1340.1 Larval Hindgut    1102.3 Larval Malpighian Tubules    862.6 Larval Fat Body    770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop    778.5 Adult Midgut    947 Adult Hindgut    632.8 Adult Malpighian Tubules    410.3 Adult Fat Body    507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca    562.2 Adult InseminatedFemale Spermatheca    595.2 Adult Ovary    965.5 Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (587.075) Larval Midgut  (1340.1) Larval Hindgut  (1102.3) Larval Malpighian Tubules  (862.6) Larval Fat Body  (770) Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop  (778.5) Adult Midgut  (947) Adult Hindgut  (632.8) Adult Malpighian Tubules    410.3 Adult Fat Body  507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca  (562.2) Adult InseminatedFemale Spermatheca  (595.2) Adult Ovary  (965.5) Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    587.075 Larval Midgut  (1340.1) Larval Hindgut  (1102.3) Larval Malpighian Tubules    862.6 Larval Fat Body    770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop    778.5 Adult Midgut    947 Adult Hindgut    632.8 Adult Malpighian Tubules    410.3 Adult Fat Body    507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca    562.2 Adult InseminatedFemale Spermatheca    595.2 Adult Ovary    965.5 Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    587.075 Larval Midgut    1340.1 Larval Hindgut    1102.3 Larval Malpighian Tubules    862.6 Larval Fat Body    770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop    778.5 Adult Midgut    947 Adult Hindgut    632.8 Adult Malpighian Tubules    410.3 Adult Fat Body    507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca    562.2 Adult InseminatedFemale Spermatheca    595.2 Adult Ovary    965.5 Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    587.075 Larval Midgut    1340.1 Larval Hindgut    1102.3 Larval Malpighian Tubules    862.6 Larval Fat Body    770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop    778.5 Adult Midgut    947 Adult Hindgut    632.8 Adult Malpighian Tubules    410.3 Adult Fat Body    507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca    562.2 Adult InseminatedFemale Spermatheca    595.2 Adult Ovary    965.5 Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  587.075 Larval Midgut  (1340.1) Larval Hindgut  (1102.3) Larval Malpighian Tubules  (862.6) Larval Fat Body  770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop  778.5 Adult Midgut  (947) Adult Hindgut  632.8 Adult Malpighian Tubules    410.3 Adult Fat Body  507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca  562.2 Adult InseminatedFemale Spermatheca  595.2 Adult Ovary  (965.5) Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    587.075 Larval Midgut  1340.1 Larval Hindgut  1102.3 Larval Malpighian Tubules    862.6 Larval Fat Body    770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop    778.5 Adult Midgut    947 Adult Hindgut    632.8 Adult Malpighian Tubules    410.3 Adult Fat Body    507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca    562.2 Adult InseminatedFemale Spermatheca    595.2 Adult Ovary    965.5 Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    587.075 Larval Midgut    1340.1 Larval Hindgut    1102.3 Larval Malpighian Tubules    862.6 Larval Fat Body    770 Larval Salivary Gland    231.2 Larval Trachea    246.95 Larval Carcass    386.3 Adult Head    294.5 Adult Eye    152.025 Adult Brain    294.2 Adult Thoracic-Abdominal Ganglion    269.3 Adult Crop    778.5 Adult Midgut    947 Adult Hindgut    632.8 Adult Malpighian Tubules    410.3 Adult Fat Body    507.8 Adult Salivary Gland    444.8 Adult Heart    317.55 Adult VirginFemale Spermatheca    562.2 Adult InseminatedFemale Spermatheca    595.2 Adult Ovary    965.5 Adult Testis    93.1 Adult Male Accessory Gland    350.4 Adult Carcass    266.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    294.5   NA  Eye    152.025   NA  Brain    294.2   587.075  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    269.3   NA  Crop    778.5   1340.1  Midgut    947   1102.3  Hindgut    632.8   862.6  Malpighian Tubules    410.3   770  Fat Body    507.8   231.2  Salivary Gland    444.8   NA  Heart    317.55   246.95  Trachea    NA   NA  VirginFemale Spermatheca    562.2   NA  InseminatedFemale Spermatheca    595.2   NA  Ovary    965.5   NA  Testis    93.1   NA  Male Accessory Gland    350.4   386.3  Carcass    266.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    58 central nervous system, pupae P8    97 head, virgin 1-day female    36 head, virgin 4-day female    33 head, virgin 20-day female    15 head, mated 1-day female    37 head, mated 4-day female    38 head, mated 20-day female    20 head, mated 1-day male    33 head, mated 4-day male    50 head, mated 20-day male    40 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    56 digestive system, larvae L3 wandering    85 digestive system, 1-day adult    55 digestive system, 4-day adult    87 digestive system, 20-day adult    56 fat body, larvae L3 wandering    30 fat body, white prepupae    100 fat body, pupae P8    39 carcass, larvae L3 wandering    31 carcass, 1-day adult    23 carcass, 4-day adult    30 carcass, 20-day adult    24 ovary, virgin 4-day female    113 ovary, mated 4-day female    155 testis, mated 4-day male    8 accessory gland, mated 4-day male    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  26 central nervous system, larvae L3  (58) central nervous system, pupae P8  (97) head, virgin 1-day female  (36) head, virgin 4-day female  (33) head, virgin 20-day female    15 head, mated 1-day female  (37) head, mated 4-day female  (38) head, mated 20-day female    20 head, mated 1-day male  (33) head, mated 4-day male  (50) head, mated 20-day male  (40) salivary gland, larvae L3 wandering    13 salivary gland, white prepupae  (56) digestive system, larvae L3 wandering  (85) digestive system, 1-day adult  (55) digestive system, 4-day adult  (87) digestive system, 20-day adult  (56) fat body, larvae L3 wandering  (30) fat body, white prepupae  (100) fat body, pupae P8  (39) carcass, larvae L3 wandering  (31) carcass, 1-day adult    23 carcass, 4-day adult  (30) carcass, 20-day adult    24 ovary, virgin 4-day female  (113) ovary, mated 4-day female  (155) testis, mated 4-day male    8 accessory gland, mated 4-day male  (44) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    58 central nervous system, pupae P8    97 head, virgin 1-day female    36 head, virgin 4-day female    33 head, virgin 20-day female    15 head, mated 1-day female    37 head, mated 4-day female    38 head, mated 20-day female    20 head, mated 1-day male    33 head, mated 4-day male    50 head, mated 20-day male    40 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    56 digestive system, larvae L3 wandering    85 digestive system, 1-day adult    55 digestive system, 4-day adult    87 digestive system, 20-day adult    56 fat body, larvae L3 wandering    30 fat body, white prepupae    100 fat body, pupae P8    39 carcass, larvae L3 wandering    31 carcass, 1-day adult    23 carcass, 4-day adult    30 carcass, 20-day adult    24 ovary, virgin 4-day female  (113) ovary, mated 4-day female  (155) testis, mated 4-day male    8 accessory gland, mated 4-day male    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    58 central nervous system, pupae P8    97 head, virgin 1-day female    36 head, virgin 4-day female    33 head, virgin 20-day female    15 head, mated 1-day female    37 head, mated 4-day female    38 head, mated 20-day female    20 head, mated 1-day male    33 head, mated 4-day male    50 head, mated 20-day male    40 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    56 digestive system, larvae L3 wandering    85 digestive system, 1-day adult    55 digestive system, 4-day adult    87 digestive system, 20-day adult    56 fat body, larvae L3 wandering    30 fat body, white prepupae    100 fat body, pupae P8    39 carcass, larvae L3 wandering    31 carcass, 1-day adult    23 carcass, 4-day adult    30 carcass, 20-day adult    24 ovary, virgin 4-day female    113 ovary, mated 4-day female    155 testis, mated 4-day male    8 accessory gland, mated 4-day male    44 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    58 central nervous system, pupae P8    97 head, virgin 1-day female    36 head, virgin 4-day female    33 head, virgin 20-day female    15 head, mated 1-day female    37 head, mated 4-day female    38 head, mated 20-day female    20 head, mated 1-day male    33 head, mated 4-day male    50 head, mated 20-day male    40 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    56 digestive system, larvae L3 wandering    85 digestive system, 1-day adult    55 digestive system, 4-day adult    87 digestive system, 20-day adult    56 fat body, larvae L3 wandering    30 fat body, white prepupae    100 fat body, pupae P8    39 carcass, larvae L3 wandering    31 carcass, 1-day adult    23 carcass, 4-day adult    30 carcass, 20-day adult    24 ovary, virgin 4-day female    113 ovary, mated 4-day female    155 testis, mated 4-day male    8 accessory gland, mated 4-day male    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  26 central nervous system, larvae L3  (58) central nervous system, pupae P8  (97) head, virgin 1-day female  (36) head, virgin 4-day female  (33) head, virgin 20-day female    15 head, mated 1-day female  (37) head, mated 4-day female  (38) head, mated 20-day female    20 head, mated 1-day male  (33) head, mated 4-day male  (50) head, mated 20-day male  (40) salivary gland, larvae L3 wandering    13 salivary gland, white prepupae  (56) digestive system, larvae L3 wandering  (85) digestive system, 1-day adult  (55) digestive system, 4-day adult  (87) digestive system, 20-day adult  (56) fat body, larvae L3 wandering  30 fat body, white prepupae  (100) fat body, pupae P8  (39) carcass, larvae L3 wandering  31 carcass, 1-day adult    23 carcass, 4-day adult  30 carcass, 20-day adult    24 ovary, virgin 4-day female  (113) ovary, mated 4-day female  (155) testis, mated 4-day male    8 accessory gland, mated 4-day male  (44) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    58 central nervous system, pupae P8    97 head, virgin 1-day female    36 head, virgin 4-day female    33 head, virgin 20-day female    15 head, mated 1-day female    37 head, mated 4-day female    38 head, mated 20-day female    20 head, mated 1-day male    33 head, mated 4-day male    50 head, mated 20-day male    40 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    56 digestive system, larvae L3 wandering    85 digestive system, 1-day adult    55 digestive system, 4-day adult    87 digestive system, 20-day adult    56 fat body, larvae L3 wandering    30 fat body, white prepupae    100 fat body, pupae P8    39 carcass, larvae L3 wandering    31 carcass, 1-day adult    23 carcass, 4-day adult    30 carcass, 20-day adult    24 ovary, virgin 4-day female  113 ovary, mated 4-day female  (155) testis, mated 4-day male    8 accessory gland, mated 4-day male    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    26 central nervous system, larvae L3    58 central nervous system, pupae P8    97 head, virgin 1-day female    36 head, virgin 4-day female    33 head, virgin 20-day female    15 head, mated 1-day female    37 head, mated 4-day female    38 head, mated 20-day female    20 head, mated 1-day male    33 head, mated 4-day male    50 head, mated 20-day male    40 salivary gland, larvae L3 wandering    13 salivary gland, white prepupae    56 digestive system, larvae L3 wandering    85 digestive system, 1-day adult    55 digestive system, 4-day adult    87 digestive system, 20-day adult    56 fat body, larvae L3 wandering    30 fat body, white prepupae    100 fat body, pupae P8    39 carcass, larvae L3 wandering    31 carcass, 1-day adult    23 carcass, 4-day adult    30 carcass, 20-day adult    24 ovary, virgin 4-day female    113 ovary, mated 4-day female    155 testis, mated 4-day male    8 accessory gland, mated 4-day male    44 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    47 embryo 02-04hr    21 embryo 04-06hr    31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr    27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr    28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new    33 white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP    30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day    33 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (47) embryo 02-04hr    21 embryo 04-06hr  (31) embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr  (27) embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr  (28) larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new  (33) white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP  (30) pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day  (33) adult female 30day  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    47 embryo 02-04hr    21 embryo 04-06hr    31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr    27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr    28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new    33 white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP    30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day    33 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    47 embryo 02-04hr    21 embryo 04-06hr    31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr    27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr    28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new    33 white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP    30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day    33 adult female 30day    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    47 embryo 02-04hr    21 embryo 04-06hr    31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr    27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr    28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new    33 white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP    30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day    33 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (47) embryo 02-04hr    21 embryo 04-06hr  31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr  27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr  28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new  (33) white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP  30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day  (33) adult female 30day  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    47 embryo 02-04hr    21 embryo 04-06hr    31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr    27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr    28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new    33 white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP    30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day    33 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    47 embryo 02-04hr    21 embryo 04-06hr    31 embryo 06-08hr    23 embryo 08-10hr    22 embryo 10-12hr    22 embryo 12-14hr    27 embryo 14-16hr    17 embryo 16-18hr    21 embryo 18-20hr    20 embryo 20-22hr    23 embryo 22-24hr    28 larva L1    15 larva L2    16 larva L3 12hr old    12 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    22 white prepupae new    33 white prepupae 12hr    21 white prepupae 24hr    17 pupae 2d postWPP    30 pupae 3d postWPP    12 pupae 4d postWPP    9 adult male 01day    11 adult male 05day    12 adult male 30day    10 adult female 01day    14 adult female 05day    33 adult female 30day    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    61 embryonic 1182-4H    34 embryonic GM2    40 embryonic Kc167    91 embryonic S1    78 embryonic S3    69 leg disc CME L1    92 wing disc CME-W2    91 wing disc ML-DmD8    39 wing disc ML-DmD9    47 wing disc ML-DmD16-c3    80 wing disc ML-DmD21    42 wing disc ML-DmD32    72 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    61 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    55 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    193 ovary fGS/OSS    46 ovary OSC    73 ovary OSS    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4  (61) embryonic 1182-4H  (34) embryonic GM2  (40) embryonic Kc167  (91) embryonic S1  (78) embryonic S3  (69) leg disc CME L1  (92) wing disc CME-W2  (91) wing disc ML-DmD8  (39) wing disc ML-DmD9  (47) wing disc ML-DmD16-c3  (80) wing disc ML-DmD21  (42) wing disc ML-DmD32  (72) haltere disc ML-DmD17-c3  (88) eye-antennal disc ML-DmD11  (61) antennal disc ML-DmD20-c5  (44) mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1  (55) CNS ML-DmBG2-c2  (71) tumorous blood cells mbn2  (193) ovary fGS/OSS  (46) ovary OSC  (73) ovary OSS  (71) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4    61 embryonic 1182-4H    34 embryonic GM2    40 embryonic Kc167    91 embryonic S1    78 embryonic S3    69 leg disc CME L1    92 wing disc CME-W2    91 wing disc ML-DmD8    39 wing disc ML-DmD9    47 wing disc ML-DmD16-c3    80 wing disc ML-DmD21    42 wing disc ML-DmD32    72 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    61 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    55 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2  (193) ovary fGS/OSS    46 ovary OSC    73 ovary OSS    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    61 embryonic 1182-4H    34 embryonic GM2    40 embryonic Kc167    91 embryonic S1    78 embryonic S3    69 leg disc CME L1    92 wing disc CME-W2    91 wing disc ML-DmD8    39 wing disc ML-DmD9    47 wing disc ML-DmD16-c3    80 wing disc ML-DmD21    42 wing disc ML-DmD32    72 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    61 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    55 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    193 ovary fGS/OSS    46 ovary OSC    73 ovary OSS    71 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    61 embryonic 1182-4H    34 embryonic GM2    40 embryonic Kc167    91 embryonic S1    78 embryonic S3    69 leg disc CME L1    92 wing disc CME-W2    91 wing disc ML-DmD8    39 wing disc ML-DmD9    47 wing disc ML-DmD16-c3    80 wing disc ML-DmD21    42 wing disc ML-DmD32    72 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    61 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    55 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    193 ovary fGS/OSS    46 ovary OSC    73 ovary OSS    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4  (61) embryonic 1182-4H  (34) embryonic GM2  (40) embryonic Kc167  (91) embryonic S1  (78) embryonic S3  (69) leg disc CME L1  (92) wing disc CME-W2  (91) wing disc ML-DmD8  (39) wing disc ML-DmD9  (47) wing disc ML-DmD16-c3  (80) wing disc ML-DmD21  (42) wing disc ML-DmD32  (72) haltere disc ML-DmD17-c3  (88) eye-antennal disc ML-DmD11  (61) antennal disc ML-DmD20-c5  (44) mixed discs ML-DmD4-c1  (54) CNS ML-DmBG1-c1  (55) CNS ML-DmBG2-c2  (71) tumorous blood cells mbn2  (193) ovary fGS/OSS  (46) ovary OSC  (73) ovary OSS  (71) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (150) Schneider line 2 Sg4    61 embryonic 1182-4H    34 embryonic GM2    40 embryonic Kc167    91 embryonic S1    78 embryonic S3    69 leg disc CME L1    92 wing disc CME-W2    91 wing disc ML-DmD8    39 wing disc ML-DmD9    47 wing disc ML-DmD16-c3    80 wing disc ML-DmD21    42 wing disc ML-DmD32    72 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    61 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    55 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2  (193) ovary fGS/OSS    46 ovary OSC    73 ovary OSS    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    150 Schneider line 2 Sg4    61 embryonic 1182-4H    34 embryonic GM2    40 embryonic Kc167    91 embryonic S1    78 embryonic S3    69 leg disc CME L1    92 wing disc CME-W2    91 wing disc ML-DmD8    39 wing disc ML-DmD9    47 wing disc ML-DmD16-c3    80 wing disc ML-DmD21    42 wing disc ML-DmD32    72 haltere disc ML-DmD17-c3    88 eye-antennal disc ML-DmD11    61 antennal disc ML-DmD20-c5    44 mixed discs ML-DmD4-c1    54 CNS ML-DmBG1-c1    55 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    193 ovary fGS/OSS    46 ovary OSC    73 ovary OSS    71 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    47 cold shock, 4-day adult    34 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    63 Cadmium 100 mM 48 hrs, 4-day adult    115 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    181 Zinc 5 mM 12 hrs, larvae L3    33 Zinc 4.5 mM 48 hrs, 4-day adult    92 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    33 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    111 Caffeine 25 mg/ml 48 hrs, 4-day adult    76 Paraquat 5 mM 48 hrs, 4-day adult    76 Paraquat 10 mM 48 hrs, 4-day adult    49 Rotenone 2 μg 12 hrs, larvae L3    35 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (47) cold shock, 4-day adult  (34) heat shock, 4-day adult  (51) Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3  (44) Cadmium 50 mM 48 hrs, 4-day adult  (63) Cadmium 100 mM 48 hrs, 4-day adult  (115) Copper 0.5 mM 12 hrs, larvae L3  (35) Copper 15 mM 48 hrs, 4-day adult  (181) Zinc 5 mM 12 hrs, larvae L3  (33) Zinc 4.5 mM 48 hrs, 4-day adult  (92) Ethanol 2.5% 3 hrs, larvae L3  (56) Ethanol 5% 3 hrs, larvae L3  (36) Ethanol 10% 3 hrs, larvae L3  (33) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (28) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (111) Caffeine 25 mg/ml 48 hrs, 4-day adult  (76) Paraquat 5 mM 48 hrs, 4-day adult  (76) Paraquat 10 mM 48 hrs, 4-day adult  (49) Rotenone 2 μg 12 hrs, larvae L3  (35) Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    47 cold shock, 4-day adult    34 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    63 Cadmium 100 mM 48 hrs, 4-day adult  (115) Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult  (181) Zinc 5 mM 12 hrs, larvae L3    33 Zinc 4.5 mM 48 hrs, 4-day adult    92 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    33 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (111) Caffeine 25 mg/ml 48 hrs, 4-day adult    76 Paraquat 5 mM 48 hrs, 4-day adult    76 Paraquat 10 mM 48 hrs, 4-day adult    49 Rotenone 2 μg 12 hrs, larvae L3    35 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    47 cold shock, 4-day adult    34 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    63 Cadmium 100 mM 48 hrs, 4-day adult    115 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    181 Zinc 5 mM 12 hrs, larvae L3    33 Zinc 4.5 mM 48 hrs, 4-day adult    92 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    33 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    111 Caffeine 25 mg/ml 48 hrs, 4-day adult    76 Paraquat 5 mM 48 hrs, 4-day adult    76 Paraquat 10 mM 48 hrs, 4-day adult    49 Rotenone 2 μg 12 hrs, larvae L3    35 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    47 cold shock, 4-day adult    34 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    63 Cadmium 100 mM 48 hrs, 4-day adult    115 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    181 Zinc 5 mM 12 hrs, larvae L3    33 Zinc 4.5 mM 48 hrs, 4-day adult    92 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    33 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    111 Caffeine 25 mg/ml 48 hrs, 4-day adult    76 Paraquat 5 mM 48 hrs, 4-day adult    76 Paraquat 10 mM 48 hrs, 4-day adult    49 Rotenone 2 μg 12 hrs, larvae L3    35 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (47) cold shock, 4-day adult  (34) heat shock, 4-day adult  (51) Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3  (44) Cadmium 50 mM 48 hrs, 4-day adult  (63) Cadmium 100 mM 48 hrs, 4-day adult  (115) Copper 0.5 mM 12 hrs, larvae L3  (35) Copper 15 mM 48 hrs, 4-day adult  (181) Zinc 5 mM 12 hrs, larvae L3  (33) Zinc 4.5 mM 48 hrs, 4-day adult  (92) Ethanol 2.5% 3 hrs, larvae L3  (56) Ethanol 5% 3 hrs, larvae L3  (36) Ethanol 10% 3 hrs, larvae L3  (33) Caffeine 1.5 mg/ml 4 hrs, larvae L3  28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (111) Caffeine 25 mg/ml 48 hrs, 4-day adult  (76) Paraquat 5 mM 48 hrs, 4-day adult  (76) Paraquat 10 mM 48 hrs, 4-day adult  (49) Rotenone 2 μg 12 hrs, larvae L3  (35) Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    47 cold shock, 4-day adult    34 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    63 Cadmium 100 mM 48 hrs, 4-day adult  115 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult  (181) Zinc 5 mM 12 hrs, larvae L3    33 Zinc 4.5 mM 48 hrs, 4-day adult    92 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    33 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  111 Caffeine 25 mg/ml 48 hrs, 4-day adult    76 Paraquat 5 mM 48 hrs, 4-day adult    76 Paraquat 10 mM 48 hrs, 4-day adult    49 Rotenone 2 μg 12 hrs, larvae L3    35 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    47 cold shock, 4-day adult    34 heat shock, 4-day adult    51 Cadmium 50 mM 6 hrs, larvae L3    25 Cadmium 50 mM 12 hrs, larvae L3    44 Cadmium 50 mM 48 hrs, 4-day adult    63 Cadmium 100 mM 48 hrs, 4-day adult    115 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    181 Zinc 5 mM 12 hrs, larvae L3    33 Zinc 4.5 mM 48 hrs, 4-day adult    92 Ethanol 2.5% 3 hrs, larvae L3    56 Ethanol 5% 3 hrs, larvae L3    36 Ethanol 10% 3 hrs, larvae L3    33 Caffeine 1.5 mg/ml 4 hrs, larvae L3    28 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    111 Caffeine 25 mg/ml 48 hrs, 4-day adult    76 Paraquat 5 mM 48 hrs, 4-day adult    76 Paraquat 10 mM 48 hrs, 4-day adult    49 Rotenone 2 μg 12 hrs, larvae L3    35 Rotenone 8 μg 12 hrs, larvae L3    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_09
External Data & Images
Linkouts	BDGP expression data • LD21679 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015286 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2849-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rala Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal RalaPG69 RalaPG89 lethal, with Scer\GAL4unspecified RalaG20V.Scer\UAS lethal | larval stage RalaG0174 RalaG0373 RalaG0499 lethal | pharate adult stage RalaG0138 RalaG0501 lethal | P-stage RalaG0501 lethal | pupal stage RalaG0138 lethal | pupal stage, with Scer\GAL4pnr-MD237 RalaGD8562 lethal | recessive RalaG0174 RalaG0373 RalaG0501 RalaPG69 RalaPG89 RalaPL56#1 viable Rala35d Rala83c Rala94c RalaEE1 Ralah1 viable, with Scer\GAL4da.G32 RalaS25N.cMa.Scer\UAS Sterility Allele male sterile RalaEE1 Other Phenotypes Allele decreased cell size | somatic clone, with Scer\GAL4Act5C.PP RalaG20V.Scer\UAS increased cell death | somatic clone RalaPG69 planar polarity defective RalaEE1 planar polarity defective | somatic clone RalaEE1 visible RalaEE1 visible, with Scer\GAL469B RalaS25N.Scer\UAS visible, with Scer\GAL4GMR.PF RalaG20V.cMa.Scer\UAS visible, with Scer\GAL4sca-537.4 RalaG20V.cMa.Scer\UAS RalaS25N.cMa.Scer\UAS RalaS25N.Scer\UAS visible, with Scer\GAL4sca.PU RalaG20V.cMa.Scer\UAS RalaS25N.cMa.Scer\UAS visible | recessive Rala35d Rala83c Rala94c Phenotype manifest in Allele border follicle cell, with Scer\GAL4slbo.2.6, Scer\GAL4upd RalaS25N.Scer\UAS border follicle cell | somatic clone RalaPG69 chaeta RalaG0138 chaeta & mesothoracic tergum RalaEE1 crossvein, with Scer\GAL4en-e16E RalaG20V.Scer\UAS crossvein | supernumerary RalaS25N.Scer\UAS dorsal closure embryo, with Scer\GAL4unspecified RalaG20V.Scer\UAS embryonic/first instar larval cuticle, with Scer\GAL4unspecified RalaG20V.Scer\UAS embryonic/larval salivary gland RalaG0501 embryonic epidermis | anterior, with Scer\GAL4LE RalaG20V.Scer\UAS embryonic epidermis | anterior, with Scer\GAL4pnr-MD237 RalaG20V.Scer\UAS embryonic epidermis | dorsal, with Scer\GAL4LE RalaG20V.Scer\UAS embryonic epidermis | dorsal, with Scer\GAL4pnr-MD237 RalaG20V.Scer\UAS eye RalaG0138 RalaEE1 eye, with Scer\GAL4GMR.PF RalaG20V.cMa.Scer\UAS eye photoreceptor cell RalaEE1 head bristle RalaEE1 head bristle, with Scer\GAL4sca.PU RalaS25N.cMa.Scer\UAS humeral bristle RalaEE1 macrochaeta Rala35d Rala83c Rala94c macrochaeta, with Scer\GAL4sca-537.4 RalaS25N.cMa.Scer\UAS RalaG20V.cMa.Scer\UAS macrochaeta, with Scer\GAL4sca.PU RalaS25N.cMa.Scer\UAS RalaG20V.cMa.Scer\UAS microchaeta Rala35d Rala83c Rala94c microchaeta, with Scer\GAL4sca-537.4 RalaS25N.cMa.Scer\UAS microchaeta, with Scer\GAL4sca.PU RalaS25N.cMa.Scer\UAS ommatidial precursor cluster RalaEE1 ommatidium RalaEE1 ommatidium | somatic clone RalaEE1 scutum & macrochaeta, with Scer\GAL469B RalaS25N.Scer\UAS scutum & macrochaeta, with Scer\GAL4sca-537.4 RalaS25N.Scer\UAS scutum & macrochaeta & tormogen cell, with Scer\GAL4sca-537.4 RalaS25N.Scer\UAS scutum & macrochaeta & trichogen cell, with Scer\GAL4sca-537.4 RalaS25N.Scer\UAS scutum & microchaeta, with Scer\GAL4sca-537.4 RalaS25N.Scer\UAS scutum & microchaeta & tormogen cell, with Scer\GAL4sca-537.4 RalaS25N.Scer\UAS scutum & microchaeta & trichogen cell, with Scer\GAL4sca-537.4 RalaS25N.Scer\UAS scutum & trichome, with Scer\GAL469B RalaS25N.Scer\UAS trichogen cell Rala35d Rala83c Rala94c trichogen cell, with Scer\GAL4sca-537.4 RalaS25N.cMa.Scer\UAS wing blade RalaEE1 wing hair, with Scer\GAL469B RalaS25N.Scer\UAS wing vein RalaG0138
Classical Alleles ( 21 )
For All Classical Alleles Show
Allele of Rala Class Mutagen Stocks Known lesion RalaG0501 P-element activity 2 -- Ralad09026 P-element activity 1 -- RalaEE1 ethyl methanesulfonate 1 Yes Ralaf05303 piggyBac transposase 1 -- Ralaf06063 piggyBac transposase 1 -- Ralaf07953 piggyBac transposase 1 -- RalaG0174 P-element activity 1 -- RalaG0373 P-element activity 1 -- RalaKG06114 P-element activity 1 -- RalaNP1083 1 -- Rala35d hypomorphic allele - molecular evidence P-element activity 0 Yes Rala83c hypomorphic allele - molecular evidence P-element activity 0 Yes Rala94c hypomorphic allele - molecular evidence P-element activity 0 Yes Ralaf02937 0 -- RalaG0138 P-element activity 0 -- RalaG0499 P-element activity 0 -- Ralah1 P-element activity 0 Yes RalaPG69 Delta2-3 0 Yes RalaPG89 Delta2-3 0 Yes RalaPL56#1 Delta2-3 0 -- Ralaunspecified 0 --
Alleles Carried on Transgenic Constructs ( 16 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rala Class Mutagen Stocks Known lesion RalaGD8562 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes RalaHMS01365 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RalaJF03259 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RalaKK108989 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RalaS25N.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 1 Yes RalaC.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RaladsRNA.cCa in vitro construct - RNAi 0 Yes RaladsRNA.cMa in vitro construct - RNAi 0 Yes RalaG20V.cMa.Scer\UAS in vitro construct - regulatory fusion 0 Yes RalaG20V.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RalaN.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RalaNIG.2849R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RalaS25N.cMa.Scer\UAS in vitro construct - regulatory fusion 0 Yes RalaScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes RalaScer\UAS.cSa in vitro construct - regulatory fusion 0 Yes RalaΔCTLL.N.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes
Aneuploid Aberrations
Duplicated in	Dp(1;3)w+67k (Eun et al., 2007) Tp(1;2)w-ec (Eun et al., 2007)
Disrupted in	Df(1)Exel6233 (Eun et al., 2007) Df(1)N-264-105 (Eun et al., 2007) Df(1)RR62 (Eun et al., 2007)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD8562} NA P{GD10692} NA P{KK108989} NA P{NIG.2849R} NA P{TRiP.HMS01365} NA P{TRiP.JF03259} NA P{UAS-Ral.G20V.M} NA P{UAS-Ral.M} NA P{UAS-Ral.S25N.M} NA P{UAS-Rala.G20V} NA P{UAS-Rala.GFP.C} NA P{UAS-Rala.GFP.N} NA P{UAS-Rala.GFP.ΔCTLL} NA P{UAS-Rala.S25N} NA P{UAS-Rala.S} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{?GawB}Rala35d NA P{?GawB}Rala83c NA P{?GawB}Rala94c NA P{GT1}BG02491 NA P{GT1}BG02491BG02491 NA P{SUPor-P}RalaKG06114 NA PBac{WH}Ralaf02937 NA PBac{WH}Ralaf05303 NA PBac{WH}Ralaf06063 NA PBac{WH}Ralaf07953 NA insertion of enhancer trap binary system P{GawB}RalaNP1083 No P{GawB}RalaPG69 No P{GawB}RalaPG89 No insertion of mobile activating element P{XP}Ralad09026 NA insertion of enhancer trap P{lacW}RalaG0138 No P{lacW}RalaG0174 No P{lacW}RalaG0373 No P{lacW}RalaG0499 No P{lacW}RalaG0501 No P{lacW}RalaPL56#1 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from direct assay (Sawamoto et al., 1999) PDZ domain binding inferred from physical interaction with Magi (Beller et al., 2002) protein binding inferred from physical interaction with Rlip (Mirey et al., 2003)
Biological Process
CV term Evidence References
border follicle cell migration inferred from mutant phenotype (Ghiglione et al., 2008) GTP catabolic process inferred from direct assay (Sawamoto et al., 1999) innate immune response inferred from direct assay (Chien et al., 2006) negative regulation of JNK cascade inferred from genetic interaction with bsk (Sawamoto et al., 1999, Balakireva et al., 2006) inferred from genetic interaction with hep (Sawamoto et al., 1999) inferred from genetic interaction with Jra (Balakireva et al., 2006) inferred from genetic interaction with kay (Balakireva et al., 2006) inferred from genetic interaction with msn (Balakireva et al., 2006) inferred from genetic interaction with Traf4 (Balakireva et al., 2006) R3/R4 cell fate commitment inferred from mutant phenotype (Cho and Fischer, 2011) Ras protein signal transduction inferred from genetic interaction with Rgl (Mirey et al., 2003) regulation of cell morphogenesis inferred from mutant phenotype (Sawamoto et al., 1999) regulation of Notch signaling pathway inferred from genetic interaction with N (Cho and Fischer, 2011)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Ghiglione et al., 2008)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
GTPase activity inferred from sequence or structural similarity (Jekely, 2003) inferred from sequence or structural similarity with UniProtKB:P05810 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 1996.2.1) GTP binding inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR005225, InterPro:IPR020849 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
dorsal closure non-traceable author statement (Harden, 2002) negative regulation of JNK cascade traceable author statement (Harden, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rala - 14-3-3ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - Arpc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG13472 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG3224 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG4612 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - btz anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - ine anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - isoQC anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - mRpS7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - rho anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rala - tyn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rala allele Gene References RalaS25N.Scer\UAS bsk (Sawamoto et al., 1999) hep (Sawamoto et al., 1999) unspecified lqf (Eun et al., 2007) trc (He et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57853 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015286 DroID - A comprehensive database of gene and protein interactions. • FBgn0015286 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31332
Orthologs
OrthoDB Orthologs (44) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6CC4MN Dipteran inclusive ortholog search EOG63FH5V Insect inclusive ortholog search EOG62V7CN Arthropod inclusive ortholog search EOG67H46C Metazoa inclusive ortholog search EOG6C2W59
Orthologs in Drosophila Species (EOG6CC4MN)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rala     Drosophila simulans   Dsim\GD16321 Y   Drosophila sechellia   Dsec\GM12716 Y   Drosophila erecta   Dere\GG18572 Y   Drosophila yakuba   Dyak\GE16885 Y   Drosophila ananassae   Dana\GF22303 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26719     Drosophila pseudoobscura pseudoobscura   Dpse\GA15484 Y   Drosophila persimilis   Dper\GL23727 Y   Drosophila willistoni   Dwil\GK24932 Y   Drosophila virilis   Dvir\GJ18826 Y   Drosophila mojavensis   Dmoj\GI14784 Y   Drosophila grimshawi   Dgri\GH24739 Y
Orthologs in non-Drosophila Dipterans (EOG63FH5V)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL014749   Anopheles gambiae Malaria mosquito  Agam\AGAP012108   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ012257
Orthologs in non-Dipteran Insects (EOG62V7CN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Ame.15888   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012128   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH20559   Atta cephalotes Leafcutter ant  Acep\ACEP26442   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12621   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13473   Linepithema humile Argentine ant  Lhum\LH21970   Pogonomyrmex barbatus Red harvester ant  Pbar\PB16069   Solenopsis invicta Red fire ant  Sinv\SINV25482   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005529   Bombyx mori Silkmoth  Bmor\BGIBMGA009918   Pediculus humanus Human body louse  Phum\PHUM256760   Tribolium castaneum Red flour beetle  Tcas\TC012218
Orthologs in non-Insect Arthropods (EOG67H46C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_303962   Ixodes scapularis Deer tick  Isca\ISCW014484
Orthologs in non-Arthropod Metazoa (EOG6C2W59)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ral-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017972gn   Danio rerio Zebrafish  Drer\rala   Danio rerio Zebrafish  Drer\ralba   Danio rerio Zebrafish  Drer\CH211-202I17.1   Xenopus tropicalis Western clawed frog  Xtro\rala   Gallus gallus Domestic chicken  Ggal\RALA   Mus musculus House mouse  Mmus\Rala   Mus musculus House mouse  Mmus\Ralb   Rattus norvegicus Norway rat  Rnor\Ralb   Rattus norvegicus Norway rat  Rnor\Rala   Homo sapiens Human  Hsap\RALB   Homo sapiens Human  Hsap\RALA
Human Orthologs (2)
Gene OMIM HGNC Hsap\RALB     Hsap\RALA
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16321 Drosophila sechellia Dsec\GM12716 Drosophila erecta Dere\GG18572 Drosophila yakuba Dyak\GE16885 Drosophila ananassae Dana\GF22303 Drosophila pseudoobscura pseudoobscura Dpse\GA15484 Drosophila persimilis Dper\GL23727 Drosophila willistoni Dwil\GK24932 Drosophila virilis Dvir\GJ18826 Drosophila mojavensis Dmoj\GI14784 Drosophila grimshawi Dgri\GH24739
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	25095 RalaEE1/FM7c 32094 w*; P{UAS-Rala.S25N}3 14142 y1 P{SUPor-P}RalaKG06114 29580 y1 v1; P{TRiP.JF03259}attP2 11920 w67c23 P{lacW}RalaG0174/FM7c
Harvard	- P{XP}Ralad09026 - PBac{WH}Ralaf05303 - PBac{WH}Ralaf06063 - PBac{WH}Ralaf07953
VDRC	v105296 P{KK108989}VIE-260B v43622 w1118; P{GD8562}v43622 v43623 w1118; P{GD8562}v43623/CyO
	More stocks available...
Genomic Clones ( 1 )
	BACR32E02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 192 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD21679
Other clones	DPiM_LD21679
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH10541 GH13852 GM05795 LD15393 LD19222 LD26273 RE07042 RE09518 RE10356 RE10510 RE10783 RE11901 RE14335 RE14550 RE15596 RE15951 RE16292 RE16433 RE16855 RE17033 RE19152 RE19719 RE24917 RE30986 RE34094 RE37275 RE39285 RE40827 RE45935 RE46083 RE46804 RE46886 RE47953 RE49034 RE49134 RE49173 RE49726 RE52607 RE55209 RE60458 RE61448 RE61789 RE61796 RE63234 RE66444 RE69955 RE71920 RE74308 RE74582 RH09967 RH13637 RH14212 SD01661
Other clones	2074 2216 9246 9538 18844 18946 30861 42994 43881 61871 61923 64905 69781 70806 70870 76206 80781 102227 104555 108763 109040 125455 148270 149589 153529 161614 167418.1 176064 176210 192561 193882 214481.56 273250 273499 277498 281971 283127 523476 533495 572934 CK01.42A.C3 CK01.59B.F3 CK01.60C.B4 CK01.66D.A12 CK01.69C.D7 CK01.70C.H2 CK01.86A.D10 CK01.103D.G6 CK01.105C.F12 CK01.109B.C4 CK01.120A.D10 CK01.145B.G5 CK01.147A.E6 Dmel_neb_008969_0954_0808 Dmel_neb_014953_0081_0944 EC14485 EC37901 EK048014 EK048022 EK053936 EK054723 EK055516 EK056161 EK063638 EK067947 EK087059 EK088354 EK098842 EK107811 EK107836 EK108411 EK120151 EK123456 EK125258 EK133424 EK133442 EK135358 EK140812 EK157953 EK159505 EK160335 EK185452 EK191623 EK198442 EK198443 EK205513 EK213820 EK235440 EK247629 EK253647 EK255335 EK256823 EK264053 EK268041 EK272212 EK280717 EK295427 EK307545 EK307964 EN01804 EN03961 EN09624 EN17537 EP02027 EP05745 EP19756 EP19915 FGM215H12 FGM314D12 RC0-DA0001-120700-012-A07 RC0-DL0001-140700-011-F07 RC0-DL0001-140700-012-G05 RC0-DL0001-140700-114-H02 RC0-DL0001-140700-118-A02 RP004071606 RP004081683 RP004130401 RP004135652 RP004182152 RP004201623 RP004214435 RP004215894 RP004219874 RP004222693 RP004235473 RP004237931 RP004242250 RP004247143 RP004249752 RP004255113 RP004261489 RP004266017 RP004342253 RP004449835 RP004475917 RP004510160 RP004523155
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015286 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31332
Antibody Information
	polyclonal (Ghiglione et al., 2008)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: Rala anon-EST:fe1H4 (FlyBase, 1996-) Source for merge of: Rala l(1)G0174 l(1)G0373 l(1)G0501 (Peter et al., 2002)
Additional comments
Other Comments
	Rala represses ligand-independent activation of N in the developing R3 photoreceptor cell. (Cho and Fischer, 2011) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Rala regulates developmental cell shape changes through the JNK pathway. (Sawamoto et al., 1999)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080324 NM_166985 NM_166986 RefSeq (Proteins) • NP_525063 NP_726881 NP_726882 DDBJ / EMBL / GenBank • AA433231 AA735651 AAC34902 AAF45889 AAF45890 AAK00587 AAM50772 AAN09102 AI455190 AJ300091 AJ300092 AJ300094 AY024366 AY118912 BH840647 CZ476521 CZ487923 CZ488703 CZ490658 U23800 Z31773 Entrez Gene - A searchable database of RefSeq genes. • 31332 UniProtKB/Swiss-Prot • P48555 UniProtKB/TrEMBL • Q962I2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Small GTPase superfamily (IPR001806) Small GTP-binding protein domain (IPR005225) P-loop containing nucleoside triphosphate hydrolase (IPR027417) Small GTPase superfamily, Ras type (IPR020849) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD21679
Linkouts
	BioGRID - A database of protein and genetic interactions • 57853 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015286 DroID - A comprehensive database of gene and protein interactions. • FBgn0015286 DRSC - Results from RNAi screens. • FBgn0015286 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015286 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2849-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015286 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31332 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/ral.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31332 modMine - Data generated by the modENCODE project. • FBgn0015286
Synonyms & Secondary IDs ( 25 )
Reported As
Symbol Synonym	24639550 (Rojas et al., 2012) anon-EST:fe1H4   CG2849 (Bourbon et al., 2002, Walker et al., 2006, Stuart et al., 2007, Larschan et al., 2007, Ghiglione et al., 2008, Keleman et al., 2009.8.5, Gandhi et al., 2007, Perkins et al., 2009, Ni et al., 2010.12.1, Cammarato et al., 2011, Colicelli, 2004) Dm RalA (Jekely, 2003) dRal (Brentrup and Jackle, 2003, Beller et al., 2002, Beller et al., 2002, Prober and Edgar, 2002, Beller et al., 2002, Mirey et al., 1999, Mirey et al., 1999, Camonis, 1999.4.6) DRal (Blanke et al., 2001, Sawamoto et al., 1999, Blanke and Jackle, 2006) Dral (He et al., 2005) DRala (Chen et al., 2002) Drala (He and Adler, 2003) D-RalA (Ryabinina et al., 2006) DralA (Ghiglione et al., 2008) l(1)G0174 (Schaefer et al., 1999.6.15, ) l(1)G0373 (Schaefer et al., 1999.8.12, ) l(1)G0501 (Schaefer et al., 1999.12.2, ) P48555 (Rojas et al., 2012) ral (Gagic et al., 2005, Balakireva et al., 2007) Ral (Geisbrecht and Montell, 2004, Balakireva et al., 2003, Gagic and Mueller, 2004, Moskalenko et al., 2002, de Bruyn et al., 2000, Chien et al., 2006, Balakireva et al., 2007, Beraud et al., 2007, Mirey et al., 2003, Balakireva et al., 2006, Lavery et al., 2007, Cho and Fischer, 2011) RalA (Jasper et al., 2001, Ghiglione et al., 2008, Dupuy et al., 2005) Ral-a (Bourbon et al., 2002) RALA (Winge, 1995.3.30) Rala (Badal et al., 2006, Walker et al., 2006, Eun et al., 2007, Stuart et al., 2007, Wang et al., 2006, Maines et al., 2007, Hazelett et al., 2012, Robida et al., 2007, Wang et al., 2008, Schwartz et al., 2009, Cronin et al., 2009, Li et al., 2009, Fernández-Ayala et al., 2010, Abruzzi et al., 2011, Japanese National Institute of Genetics, 2012.5.21) ralA (Loo et al., 2005)
Name Synonym	anon-fast-evolving-1H4   Ras-like protein A   Ras-related protein (Cammarato et al., 2011, Sambandan et al., 2006)
Secondary FlyBase IDs
	FBgn0025241 FBgn0027285 FBgn0028308 FBgn0040134
References ( 93 )
Generate a list of	All references relating to this gene ( 93 )
List References by type	Research paper  ( 43 ) Supplementary material  ( 14 ) Review  ( 3 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 11 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Hazelett et al., 2012, G3 (Bethesda) 2(7): 789--802 Comparison of Parallel High-Throughput RNA Sequencing Between Knockout of TDP-43 and Its Overexpression Reveals Primarily Nonreciprocal and Nonoverlapping Gene Expression Changes in the Central Nervous System of Drosophila. [FBrf0219102] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386 Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Carmena et al., 2011, J. Cell Biol. 195(4): 553--562 The Rap1-Rgl-Ral signaling network regulates neuroblast cortical polarity and spindle orientation. [FBrf0216601] Cho and Fischer, 2011, Development 138(7): 1349--1359 Ral GTPase promotes asymmetric Notch activation in the Drosophila eye in response to Frizzled/PCP signaling by repressing ligand-independent receptor activation. [FBrf0213208] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...