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Gene Dmel\U2af65

General Information
Symbol	Dmel\U2af65	Species	D. melanogaster
Name	U2 small nuclear riboprotein auxiliary factor 65	Annotation symbol
Feature type	gene	FlyBase ID	FBgn0015318
Gene Model Status	Unannotated	Stock availability	None publicly available
Also Known As	U2AF, U2AF65
Genomic Location
Chromosome (arm)		Recombination map
Cytogenetic map		Sequence location
Summary Information
Automatically generated summary See sections below for more information	The gene U2 small nuclear riboprotein auxiliary factor 65 is referred to in FlyBase by the symbol Dmel\U2af65 (FBgn0015318). It is a gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: poly-pyrimidine tract binding. The biological processes in which it is involved are not known. No alleles are reported. It has no annotated transcripts. Gene has not been localized to the genome sequence.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein
Additional Polypeptide Data & Comments
Reported size (kDa)	65 (kD) (Valcarcel et al., 1993)
Comments	U2af65 protein binds to the non-sex specific pyrimidine tract/3' splice site of tra pre-mRNA. It also binds with 100 fold lower efficiency to the female-specific polypyrimidine tract. (Valcarcel et al., 1993)
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Mapped Features
Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of U2af65 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 0 )
For All Alleles Carried on Transgenic Constructs Show
Allele of U2af65 Class Mutagen Stocks Known lesion
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data characterization construct P{RS-Sx} NA P{USx} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 1 unique term )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function
CV term Evidence References
poly-pyrimidine tract binding inferred from direct assay (Singh et al., 2000)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 0 terms )
Sequence Ontology: Class of Gene
	gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions U2af65 allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
Linkouts
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 0 )
cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Essential splicing factor involved in the choice of tra splice sites in sex determination. Sxl blocks splicing to the non-sex specific, default site in tra by specifically binding to its polypyrimidine tract, blocking the binding of the essential splicing factor U2AF: U2AF then acivates the lower-affinity female-specific site. U2AF has a splicing effector domain that Sxl lacks: addition of this effector domain converts Sxl from a splicing repressor to a splicing activator, rendering it unable to mediate splice-site switching. (Valcarcel et al., 1993) Immunological and functional criteria demonstrate U2AF is evolutionarily conserved. U2AF protein activity can replace human U2AF and interact with components of the human pre-mRNA splicing machinery. (Zamore and Green, 1991) U2AF is an essential splicing factor. Purified U2AF comprises two polypeptides of 65 and 35kD, U2af65 and U2af35 respectively. (Zamore and Green, 1991) A component, with U2AF35, of the U2AF mRNA splicing factor.
External Crossreferences & Linkouts
Sequence Crossreferences
Other Crossreferences
Linkouts
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	U2AF (Smith and Valcarcel, 2000, Nagengast et al., 2004, Nagengast and Salz, 2004, Maniatis and Tasic, 2002, Graveley, 2002, Granadino et al., 1997, Zamore and Green, 1991, ) U2af65   U2AF65 (Kielkopf et al., 2004, Singh et al., 2000, Banerjee et al., 2003)
Name Synonym	U2 small nuclear riboprotein auxiliary factor 65
Secondary FlyBase IDs
	FBgn0012029
References ( 12 )
Research paper	Banerjee et al., 2003, RNA, N.Y. 9(1): 88--99 Sex lethal and U2 small nuclear ribonucleoprotein auxiliary factor (U2AF(65)) recognize polypyrimidine tracts using multiple modes of binding. [FBrf0156045] Singh et al., 2000, RNA, N.Y. 6(6): 901--911 Differential recognition of the polypyrimidine-tract by the general splicing factor U2AF65 and the splicing repressor sex-lethal. [FBrf0132065] Granadino et al., 1997, Proc. Natl. Acad. Sci. USA 94(14): 7343--7348 Distinct mechanisms of splicing regulation in vivo by the Drosophila protein Sex-lethal. [FBrf0094816] Kanaar et al., 1993, Science 262(5133): 569--573 The conserved pre-mRNA splicing factor U2AF from Drosophila: requirement for viability. [FBrf0064538] Zamore and Green, 1991, EMBO J. 10(1): 207--214 Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution. [FBrf0053877]
Review	Kielkopf et al., 2004, Genes Dev. 18(13): 1513--1526 U2AF homology motifs: protein recognition in the RRM world. [FBrf0179730] Graveley, 2002, Cell 109(4): 409--412 Sex, AGility, and the regulation of alternative splicing. [FBrf0148923] Maniatis and Tasic, 2002, Nature 418(6894): 236--243 Alternative pre-mRNA splicing and proteome expansion in metazoans. [FBrf0150778] Smith and Valcarcel, 2000, Trends Biochem. Sci. 25(8): 381--388 Alternative pre-mRNA splicing: the logic of combinatorial control. [FBrf0130331]
Abstract	Nagengast et al., 2004, A. Dros. Res. Conf. 45: 612C Building the sxl autoregulatory complex. [FBrf0173709] Nagengast and Salz, 2004, A. Dros. Res. Conf. 45: 611B SPP, a novel SR-like protein, links SEX-LETHAL to the U1 snRNP and U2AF and contributes to male exon skipping. [FBrf0173715]
Letter	Valcarcel et al., 1993, Nature 362(6416): 171--175 The protein Sex-lethal antagonizes the splicing factor U2AF to regulate alternative splicing of transformer pre-mRNA. [FBrf0059127]