FlyBase Gene Report: Dmel\Ubc2

E2 UBIQUITIN CONJUGATING ENZYMES -: Ubiquitin-conjugating (E2) enzymes accept activated ubiquitin from ubiquitin-activating (E1) enzymes via thioester bond with a conserved catalytic cysteine. E2s then interact with ubiquitin ligases (E3) to directly or indirectly ubiquitinate target proteins. E2s are characterised by a 200 amino acid ubiquitin-conjugating (UBC) domain which interacts with E1 and E3s and acts catalytically. (Adapted from PMID:1994026).

Protein Function (UniProtKB)

Catalyzes the covalent attachment of ubiquitin to other proteins.

(UniProt, P52485)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Ubc2 or the JBrowse view of Dmel\Ubc2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ubc2-RA

FBtr0080115

NM_057789

1558

232

Ubc2-RB

FBtr0080116

NM_164943

1349

232

Ubc2-RC

FBtr0333492

NM_001273433

2111

232

Ubc2-RD

FBtr0345437

NM_001298885

1625

232

Additional Transcript Data and Comments

Reported size (kB)

1.5 (northern blot)

(Matuschewski et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Ubc2-PA

FBpp0079704

24.4

232

8.47

NP_477137

AAF53008

Ubc2-PB

FBpp0079705

24.4

232

8.47

NP_723616

AAN10762

Ubc2-PC

FBpp0305676

24.4

232

8.47

NP_001260362

AGB92897

Ubc2-PD

FBpp0311562

24.4

232

8.47

NP_001285814

AHN54328

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

232 aa isoforms: Ubc2-PA, Ubc2-PB, Ubc2-PC, Ubc2-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

232 (aa); 24.5 (kD predicted)

(Matuschewski et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ubc2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (4 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

ubiquitin-protein transferase activity

inferred from genetic interaction with SGD:S000000286

(Matuschewski et al., 1996)

inferred from genetic interaction with SGD:S000002466

(Matuschewski et al., 1996)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

ubiquitin conjugating enzyme activity

inferred from biological aspect of ancestor with PANTHER:PTN002514495

(assigned by GO_Central )

(Gaudet et al., 2011)

ubiquitin-protein transferase activity

inferred from sequence or structural similarity with SGD:S000000286

(Matuschewski et al., 1996)

inferred from sequence or structural similarity with SGD:S000002466

(Matuschewski et al., 1996)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

lipid storage

inferred from direct assay

(Beller et al., 2008)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

protein polyubiquitination

inferred from sequence or structural similarity with SGD:S000002466

(FlyBase Curators, 2008--)

Cellular Component (0 terms)

There is no information supporting a Cellular Component term for this gene. (Based on a review of available data on: 2014-05-16 )

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Ubc2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ubc2

View exonic expression by tissue for Dmel\Ubc2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015320

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015320

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6720-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6720

Flygut - An atlas of the Drosophila adult midgut

FBgn0015320

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Ubc2

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ubc2

Transgenic constructs containing regulatory region of Ubc2

Deletions and Duplications ( 3 )

Disrupted in

Df(2L)BSC32

(Deal and Cook, 2003.7.11)

Df(2L)BSC214

(Christensen and Cook, 2006.8.30)

Df(2L)Exel8026

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Ubc2^k13206

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ubc2^GD14776

viable, with Scer\GAL4^Mef2.PR

Ubc2^GD14776

viable, with Scer\GAL4^pnr-MD237

Ubc2^GD14776

viable, with Scer\GAL4^tin.CΔ4

Ubc2^GD14776

Other Phenotypes

Allele

eye color defective, with Scer\GAL4^GMR.PF

Ubc2^GD14776

neuroanatomy defective | somatic clone

Ubc2^k13206

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Ubc2^GD14776

visible, with Scer\GAL4^pnr-MD237

Ubc2^GD14776

visible, with Scer\GAL4^sca-537.4

Ubc2^EP2146b

visible | adult stage, with Scer\GAL4^GMR.PF

Ubc2^GD14776

Phenotype manifest in

Allele

macrochaeta

Ubc2^L32BC

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Ubc2^GD14776

neuroblast | somatic clone

Ubc2^k13206

scutellar bristle, with Scer\GAL4^sca-537.4

Ubc2^EP2146b

tormogen cell, with Scer\GAL4^sca-537.4

Ubc2^EP2146b

trichogen cell | ectopic, with Scer\GAL4^sca-537.4

Ubc2^EP2146b

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\UBE2E2

14 of 15

Yes

Hsap\UBE2E3

14 of 15

Yes

Hsap\UBE2E1

13 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ube2e2

14 of 15

Yes

Mmus\Ube2e3

14 of 15

Yes

Mmus\Ube2e1

13 of 15

Yes

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Rattus norvegicus (Norway rat) (7)

Rnor\Ube2e1

7 of 13

Yes

Rnor\Ube2e3

7 of 13

Yes

Rnor\Ube2e2

3 of 13

Yes

2 of 13

2 of 13

2 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (7)

Xtro\ube2e3

10 of 12

Yes

Xtro\ube2e1

7 of 12

Yes

Xtro\ube2e2

5 of 12

Yes

2 of 12

2 of 12

2 of 12

1 of 12

Danio rerio (Zebrafish) (12)

Drer\ube2e2

14 of 15

Yes

Drer\ube2e3

13 of 15

Yes

Drer\ube2e1

8 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (3)

Cele\let-70

1 of 15

Yes

Cele\ubc-13

1 of 15

Yes

Cele\uev-3

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (10)

Atha\FUS9

5 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\UBC4

3 of 15

Yes

Scer\UBC5

3 of 15

Yes

Scer\PEX4

1 of 15

Yes

Scer\UBC13

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\ubc4

1 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190GCJ )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Ubc2

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19810

Drosophila persimilis

Dper\GL26387

Drosophila willistoni

Dwil\GK23983

Drosophila virilis

Dvir\GJ17643

Drosophila mojavensis

Dmoj\GI17815

Drosophila grimshawi

Dgri\GH13340

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150DYF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17024127

Glossina morsitans

Tsetse fly

Gmor\GMOY008734

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07626

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005038

Anopheles darlingi

American malaria mosquito

Adar\ADAC009100

Anopheles gambiae

Malaria mosquito

Agam\AGAP007952

Anopheles gambiae

Malaria mosquito

Agam\AGAP012754

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001502

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0IP9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA010541

Bombyx mori

Silkmoth

Bmor\BGIBMGA002918

Danaus plexippus

Monarch butterfly

Dple\DPOGS200166-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS200498-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL005107

Heliconius melpomene

Postman butterfly

Hmel\HMEL006881

Apis florea

Little honeybee

Aflo\2368864788

Apis florea

Little honeybee

Aflo\3369110196

Apis mellifera

Western honey bee

Amel\LOC724793

Apis mellifera

Western honey bee

Amel\UbcD2

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP10401

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP15298

Bombus terrestris

Buff-tailed bumblebee

Bter\15208574

Bombus terrestris

Buff-tailed bumblebee

Bter\15215993

Linepithema humile

Argentine ant

Lhum\LH13622

Linepithema humile

Argentine ant

Lhum\LH22371

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00160a

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00190e

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG007707

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG010264

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01946

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02422

Tribolium castaneum

Red flour beetle

Tcas\eff

Tribolium castaneum

Red flour beetle

Tcas\TC012100

Pediculus humanus

Human body louse

Phum\PHUM437950

Pediculus humanus

Human body louse

Phum\PHUM570280

Rhodnius prolixus

Kissing bug

Rpro\RPRC005317

Rhodnius prolixus

Kissing bug

Rpro\RPRC012234

Cimex lectularius

Bed bug

Clec\CLEC001726

Cimex lectularius

Bed bug

Clec\CLEC006604

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003002

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000078

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08928

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11733

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0DVW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR008921

Ixodes scapularis

Black-legged tick

Isca\ISCW018551

Ixodes scapularis

Black-legged tick

Isca\ISCW020158

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60987.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77107.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur348g00010

Tetranychus urticae

Two-spotted spider mite

Turt\tetur07g03260

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g02420

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_120690

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_230542

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GF8 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ube2e

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008618gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_006199gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_009521gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ubc

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ube1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_027607gn

Ciona intestinalis

Vase tunicate

Cint\LOC100183458

Ciona intestinalis

Vase tunicate

Cint\LOC100185831

Ciona intestinalis

Vase tunicate

Cint\LOC104266887

Ciona intestinalis

Vase tunicate

Cint\LOC100175624

Ciona intestinalis

Vase tunicate

Cint\LOC100185453

Gallus gallus

Domestic chicken

Ggal\UBE2E1

Gallus gallus

Domestic chicken

Ggal\UBE2D1

Gallus gallus

Domestic chicken

Ggal\UBE2E3

Gallus gallus

Domestic chicken

Ggal\UBE2D3

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (22)

Gene Symbol

Score

Source

Alignment

5 of 10

5 of 10

eff

4 of 10

CG10862

3 of 10

morgue

3 of 10

ben

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

vih

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

UBE2E2; ubiquitin conjugating enzyme E2 E2

14 of 15

602163

UBE2E3; ubiquitin conjugating enzyme E2 E3

14 of 15

604151

UBE2E1; ubiquitin conjugating enzyme E2 E1

13 of 15

602916

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ubc2 - CG44251

two hybrid

(Hu et al., 2017.6.13)

Ubc2 - Iru

two hybrid

(Hu et al., 2017.6.13)

Ubc2 - Su(dx)

two hybrid

(Hu et al., 2017.6.13)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enc

enhanceable

Ubc2

(Ohlmeyer and Schupbach, 2003)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

60561

DroID - A comprehensive database of gene and protein interactions.

FBgn0015320

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34487

MIST (protein-protein) - An integrated Molecular Interaction Database

Ubc2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ubiquitin mediated proteolysis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-43

Cytogenetic map

32A5-32A5

Sequence location

2L:10,765,010..10,768,006 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

32A5-32A5

Limits computationally determined from genome sequence between P{PZ}dbf¹ and P{lacW}SCAR^k03107

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

32A-32A

(determined by in situ hybridisation)

(Abdelilah-Seyfried et al., 2000)

32A4-32A5

(determined by in situ hybridisation)

(Spradling et al., 1999)

32A-32B

(determined by in situ hybridisation)

(Matuschewski et al., 1996)

32A4-32A5

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-43

(FlyBase, 2016)

2-36.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

11071

y¹ w^67c23; P{lacW}Ubc2^k13206/CyO

Kyoto

122055

w¹¹¹⁸; P{EP}Ubc2^EP2598

111318

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}Ubc2^k13206 P{neoFRT}40A/CyO y⁺

VDRC

v31158

w¹¹¹⁸; P{GD14776}v31158

Genomic Clones (33)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (134)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

RE74673

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015320

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015320

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34487

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Ubc2 UbcD2

(FlyBase, 2009-)

Source for database merge of

Source for merge of: UbcD2 l(2)k13206

(Spradling et al., 1999)

Additional comments

Renamed from 'UbcD2' to 'Ubc2' as the 'D' indicates 'Drosophila' (which FlyBase nomenclature rules disallow) and to avoid the implication that this is the specific ortholog of human UBE2D2.

(FlyBase, 2009-)

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

A UbcD2 cDNA has been cloned and sequenced.

(Matuschewski et al., 1996)

S.cerevisiae Scer\ubc4 Scer\ubc5 double mutants are partially functionally complemented by UbcD2.

(Matuschewski et al., 1996)

Origin and Etymology

Discoverer

Etymology

Named 'UbcD2' for 'ubiquitin-conjugating enzyme from Drosophila 2 (the numbering reflects the order of identification).

(Matuschewski et al., 1996)

Identification

External Crossreferences and Linkouts ( 68 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

34487

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P52485

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q541C3

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015320

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015320

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6720

Flygut - An atlas of the Drosophila adult midgut

FBgn0015320

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

34487

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC012100

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG6720

modMine - A data warehouse for the modENCODE project

FBgn0015320

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

P52485

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

11071-UbcD2[k13206]

BioGRID - A database of protein and genetic interactions.

60561

DroID - A comprehensive database of gene and protein interactions.

FBgn0015320

DRSC - Results frm RNAi screens

FBgn0015320

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015320

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG6720-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015320

FlyMine - An integrated database for Drosophila genomics

FBgn0015320

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

34487

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ubiquitin mediated proteolysis

MIST (protein-protein) - An integrated Molecular Interaction Database

Ubc2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG6720

(Chen et al., 2012, Zhang et al., 2011, Zhang et al., 2010, Norga et al., 2003)

UBCD2

(Daou et al., 2018)

Ubc2

(Hu et al., 2017.6.13)

UbcD2

(Hans et al., 2014, Gurudatta et al., 2012, Popkova et al., 2012, Abed et al., 2011, Barry et al., 2011, Du et al., 2011, Franco et al., 2011, Carbone et al., 2009, Michelle et al., 2009, Hoopfer et al., 2008, Altenhein et al., 2006, Christensen and Cook, 2006.8.30, Kuo et al., 2006, Matuschewski et al., 1996)

l(2)k13206

(Mesilaty-Gross et al., 1999, BDGP Project Members, 1994-1999)

ubcD2

(Kuo et al., 2006, Ryoo et al., 2002, Ryoo et al., 2002)

Name Synonyms

Ubiquitin conjugating enzyme 2

ubiquitin conjugating enzyme 2

(Beller et al., 2008)

Secondary FlyBase IDs

FBgn0021893

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (68)

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