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Gene Dmel\kek1

General Information
Symbol	Dmel\kek1	Species	D. melanogaster
Name	kekkon-1	Annotation symbol	CG12283
Feature type	protein_coding_gene	FlyBase ID	FBgn0015399
Gene Model Status	Current	Stock availability	8 publicly available
Also Known As	Kek, kek-1, kekon, CT18186
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	34A1-34A1	Sequence location	2L:12,817,000..12,822,787 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene kekkon-1 is referred to in FlyBase by the symbol Dmel\kek1 (CG12283, FBgn0015399). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: receptor antagonist activity; epidermal growth factor receptor binding. There is experimental evidence that it is involved in the biological process: negative regulation of epidermal growth factor-activated receptor activity; negative regulation of epidermal growth factor receptor signaling pathway; cytokinesis; oogenesis; neurogenesis. 42 alleles are reported. The phenotypes of these alleles are annotated with: organ system; adult segment; organ system subdivision; thoracic segment; external compound sense organ; adult; cell part; cell periphery; portion of tissue; epithelial cell; cell; peripheral nervous system. It has one annotated transcript and one annotated polypeptide. Protein features are: Cysteine-rich flanking region, C-terminal; Immunoglobulin I-set; Immunoglobulin subtype 2; Immunoglobulin-like domain; Immunoglobulin-like fold; Leucine-rich repeat; Leucine-rich repeat, typical subtype. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult brain. Comments on Affy2 ProbeSet: ProbeSet 1634071_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of kek1. Gene sequence location is 2L:12817000..12822787.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 34A1-34A1   Limits computationally determined from genome sequence between P{lacW}bunk00612&P{EP}EP682EP682 and P{lacW}l(2)k07015k07015&P{PZ}l(2)rK639rK639 34A1--2 Left limit from in situ hybridisation (FBrf0111489) Right limit from in situ hybridisation (FBrf0111489)
Experimentally Determined Cytological Location
Cytogenetic map Notes References 33F-33F     (Musacchio, 1995.12.11) 34A1-34A2   (determined by in situ hybridisation)   (Spradling et al., 1999) 33F1-33F2   (determined by in situ hybridisation)   (Musacchio and Perrimon, 1996) 34A1-34A2     (BDGP Project Members, 1994-1999)   Initial assessment of map location of gene responsible for the 'unstrung' phenotype as reported in FBrf0053298 was in error.   (Perrimon, 1998.8.10)
Experimentally Determined Recombination Data
Location	2- (Musacchio, 1995.12.11)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\kek1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Gene model reviewed during 5.46 Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) kek1-RB FBtr0332210  NM_078835   5788   880
Additional Transcript Data & Comments
Reported size (kB)	5.5 (northern blot) (Musacchio and Perrimon, 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein kek1-PB   FBpp0304518   94.5   880   7.17     NP_523559   AAF53225
Additional Polypeptide Data & Comments
Reported size (kDa)	880 (aa); 92 (kD predicted) (Musacchio and Perrimon, 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin subtype 2 (IPR003598) Cysteine-rich flanking region, C-terminal (IPR000483) Leucine-rich repeat (IPR001611) Immunoglobulin I-set (IPR013098) Immunoglobulin-like fold (IPR013783) Leucine-rich repeat, typical subtype (IPR003591) Immunoglobulin-like domain (IPR007110)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA695190   AE014134 AAF53225   AI257566   AI403771   AI404499   AI405109   AI513671   AI530905   AI542946   AQ025829   AW944198   AY095093 AAM11421   BF492675   BF492676   BG633260   BG635745   BG640268   BG640579   BI141776   BI635587   BI639091   BI640514   BP550515   BP557882   BP557883   BP559157   BT149888   CK658414   CK659077   CO153243   CO155991   CO179597   CO262663   CO266825   CO274554   CO274766   CO277707   CO277926   CO278858   CO289832   CO289857   CO300490   CO310037   CO310686   CO317865   CO319088   CO321293   CO324306   CO329359   CO338658   DN153930   DQ138830 ABA86436   EC058477   EC063311   EC076445   EC079782   EC084529   EC198550   EC202136   EC202369   EC204208   EC205773   EC209466   EC216180   EC220971   EC221422   EC226072   EC230648   EC231074   EC231075   EC232909   EC232933   EC245551   EC245798   EC246114   EC247929   EC262676   EC265348   EC265750   G00461   U42767 AAC47404
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	P91643 Q8T3J2 Q2XY32 Q9VK54
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Musacchio and Perrimon, 1996) peripheral nervous system | restricted   (Musacchio and Perrimon, 1996) antenno-maxillary complex   (Musacchio and Perrimon, 1996) central nervous system   (Musacchio and Perrimon, 1996) adult stage | female   ovary   (Musacchio and Perrimon, 1996)
Additional Descriptive Data
	kek1 transcripts are detected in9-13hr embryos on northern blots. They are detected by in situhybridization early in the midline and later in most neurons in the CNS.In the PNS, they are expressed in cap cells and weakly in ventral andlateral clusters. They are also expressed in the antenno-maxillary complexand in ovaries in a pattern similar to that of the corresponding enhancertrap line (Ecol\lacZkek1-15A6). (Musacchio and Perrimon, 1996)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
Reporter: P{lArB}kek115A6 Stage Tissue/Position (including subcellular localization) Reference embryonic stage 10 -- 11   neuroblast NB6-4   (Musacchio and Perrimon, 1996) embryonic stage 12 -- 17   central nervous system   (Musacchio and Perrimon, 1996) VUM neuron   (Musacchio and Perrimon, 1996) ventral midline   (Musacchio and Perrimon, 1996) embryonic stage 14 -- 17   presumptive embryonic/larval peripheral nervous system | restricted   (Musacchio and Perrimon, 1996) antenno-maxillary complex   (Musacchio and Perrimon, 1996) larval stage   imaginal disc   (Musacchio and Perrimon, 1996) morphogenetic furrow | posterior to   (Musacchio and Perrimon, 1996) eye-antennal disc   (Musacchio and Perrimon, 1996) ocellus   (Musacchio and Perrimon, 1996) ocellar bristle   (Musacchio and Perrimon, 1996) third instar larval stage   wing disc | restricted   (Ghiglione et al., 2003) eye disc posterior to the morphogenetic furrow   (Ghiglione et al., 2003) adult stage & oogenesis   nurse cell   (Musacchio and Perrimon, 1996) follicle cell   (Musacchio and Perrimon, 1996) border follicle cell   (Musacchio and Perrimon, 1996) oogenesis & adult stage | female   ovary   (Musacchio and Perrimon, 1996) oogenesis   germarium region 2   (Ghiglione et al., 1999) oogenesis stage S1 -- S6   egg chamber | restricted   (Ghiglione et al., 1999) Comment:at anterior and posterior poles oogenesis stage S8 -- S9   oocyte associated follicular epithelium | anterior & dorsal   (Ghiglione et al., 1999)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult brain. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    53.825 Larval Midgut    23.1 Larval Hindgut    10.9 Larval Malpighian Tubules    8.8 Larval Fat Body    20 Larval Salivary Gland    13 Larval Trachea    47.875 Larval Carcass    22.625 Adult Head    17.6 Adult Eye    30.025 Adult Brain    113.8 Adult Thoracic-Abdominal Ganglion    80.9 Adult Crop    9.4 Adult Midgut    15.2 Adult Hindgut    9.6 Adult Malpighian Tubules    10.4 Adult Fat Body    13.2 Adult Salivary Gland    15.7 Adult Heart    9.2 Adult VirginFemale Spermatheca    13.5 Adult InseminatedFemale Spermatheca    11.6 Adult Ovary    7.3 Adult Testis    11.9 Adult Male Accessory Gland    25.3 Adult Carcass    16.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    17.6   NA  Eye    30.025   NA  Brain    113.8   53.825  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    80.9   NA  Crop    9.4   23.1  Midgut    no informative data   no informative data  Hindgut    9.6   8.8  Malpighian Tubules    no informative data   no informative data  Fat Body    13.2   no informative data  Salivary Gland    no informative data   NA  Heart    9.2   47.875  Trachea    NA   NA  VirginFemale Spermatheca    13.5   NA  InseminatedFemale Spermatheca    11.6   NA  Ovary    7.3   NA  Testis    11.9   NA  Male Accessory Gland    25.3   no informative data  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8    35 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8  (35) head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8    35 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8    35 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8    35 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8  (35) head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8    35 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    23 central nervous system, larvae L3    24 central nervous system, pupae P8    35 head, virgin 1-day female    13 head, virgin 4-day female    12 head, virgin 20-day female    5 head, mated 1-day female    11 head, mated 4-day female    8 head, mated 20-day female    6 head, mated 1-day male    17 head, mated 4-day male    16 head, mated 20-day male    12 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    4 fat body, white prepupae    8 fat body, pupae P8    10 carcass, larvae L3 wandering    15 carcass, 1-day adult    1 carcass, 4-day adult    1 carcass, 20-day adult    2 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    1 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of extremely low expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    6 embryo 12-14hr    7 embryo 14-16hr    8 embryo 16-18hr    9 embryo 18-20hr    9 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    7 larva L2    2 larva L3 12hr old    1 larva L3 puffstage 1-2    3 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    9 white prepupae new    11 white prepupae 12hr    19 white prepupae 24hr    20 pupae 2d postWPP    14 pupae 3d postWPP    6 pupae 4d postWPP    4 adult male 01day    3 adult male 05day    3 adult male 30day    3 adult female 01day    3 adult female 05day    3 adult female 30day    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    68 embryonic 1182-4H    80 embryonic GM2    94 embryonic Kc167    102 embryonic S1    36 embryonic S3    11 leg disc CME L1    60 wing disc CME-W2    76 wing disc ML-DmD8    99 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    79 wing disc ML-DmD21    53 wing disc ML-DmD32    123 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    55 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    38 ovary fGS/OSS    57 ovary OSC    48 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (43) Schneider line 2 Sg4  (68) embryonic 1182-4H  (80) embryonic GM2  (94) embryonic Kc167  (102) embryonic S1  (36) embryonic S3    11 leg disc CME L1  (60) wing disc CME-W2  (76) wing disc ML-DmD8  (99) wing disc ML-DmD9  (87) wing disc ML-DmD16-c3  (79) wing disc ML-DmD21  (53) wing disc ML-DmD32  (123) haltere disc ML-DmD17-c3  (91) eye-antennal disc ML-DmD11  (69) antennal disc ML-DmD20-c5  (55) mixed discs ML-DmD4-c1  (61) CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2  (38) ovary fGS/OSS  (57) ovary OSC  (48) ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    68 embryonic 1182-4H    80 embryonic GM2    94 embryonic Kc167  102 embryonic S1    36 embryonic S3    11 leg disc CME L1    60 wing disc CME-W2    76 wing disc ML-DmD8    99 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    79 wing disc ML-DmD21    53 wing disc ML-DmD32  (123) haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    55 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    38 ovary fGS/OSS    57 ovary OSC    48 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    68 embryonic 1182-4H    80 embryonic GM2    94 embryonic Kc167    102 embryonic S1    36 embryonic S3    11 leg disc CME L1    60 wing disc CME-W2    76 wing disc ML-DmD8    99 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    79 wing disc ML-DmD21    53 wing disc ML-DmD32    123 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    55 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    38 ovary fGS/OSS    57 ovary OSC    48 ovary OSS    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    68 embryonic 1182-4H    80 embryonic GM2    94 embryonic Kc167    102 embryonic S1    36 embryonic S3    11 leg disc CME L1    60 wing disc CME-W2    76 wing disc ML-DmD8    99 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    79 wing disc ML-DmD21    53 wing disc ML-DmD32    123 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    55 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    38 ovary fGS/OSS    57 ovary OSC    48 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (43) Schneider line 2 Sg4  (68) embryonic 1182-4H  (80) embryonic GM2  (94) embryonic Kc167  (102) embryonic S1  (36) embryonic S3    11 leg disc CME L1  (60) wing disc CME-W2  (76) wing disc ML-DmD8  (99) wing disc ML-DmD9  (87) wing disc ML-DmD16-c3  (79) wing disc ML-DmD21  (53) wing disc ML-DmD32  (123) haltere disc ML-DmD17-c3  (91) eye-antennal disc ML-DmD11  (69) antennal disc ML-DmD20-c5  (55) mixed discs ML-DmD4-c1  (61) CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2  (38) ovary fGS/OSS  (57) ovary OSC  (48) ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    68 embryonic 1182-4H    80 embryonic GM2    94 embryonic Kc167  102 embryonic S1    36 embryonic S3    11 leg disc CME L1    60 wing disc CME-W2    76 wing disc ML-DmD8    99 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    79 wing disc ML-DmD21    53 wing disc ML-DmD32  123 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    55 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    38 ovary fGS/OSS    57 ovary OSC    48 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    43 Schneider line 2 Sg4    68 embryonic 1182-4H    80 embryonic GM2    94 embryonic Kc167    102 embryonic S1    36 embryonic S3    11 leg disc CME L1    60 wing disc CME-W2    76 wing disc ML-DmD8    99 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    79 wing disc ML-DmD21    53 wing disc ML-DmD32    123 haltere disc ML-DmD17-c3    91 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    55 mixed discs ML-DmD4-c1    61 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    22 tumorous blood cells mbn2    38 ovary fGS/OSS    57 ovary OSC    48 ovary OSS    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    2 cold shock, 4-day adult    2 heat shock, 4-day adult    2 Cadmium 50 mM 6 hrs, larvae L3    2 Cadmium 50 mM 12 hrs, larvae L3    3 Cadmium 50 mM 48 hrs, 4-day adult    2 Cadmium 100 mM 48 hrs, 4-day adult    4 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    4 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    2 Ethanol 5% 3 hrs, larvae L3    2 Ethanol 10% 3 hrs, larvae L3    1 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    2 Paraquat 5 mM 48 hrs, 4-day adult    2 Paraquat 10 mM 48 hrs, 4-day adult    3 Rotenone 2 μg 12 hrs, larvae L3    2 Rotenone 8 μg 12 hrs, larvae L3    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_25
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015399 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12283-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal kek1GD28 lethal, with Scer\GAL4Act5C.PI kek1Scer\UAS.T:Avic\GFP lethal | recessive kek13K2S kek13K2X kek1RA5 kek1RJ22 kek1RK9 kek1RM2 viable, with Scer\GAL4pnr-MD237 kek1GD2524 kek1GD14381 Other Phenotypes Allele neuroanatomy defective | larval stage kek1GD28 visible, with Scer\GAL471B kek1Scer\UAS.cGa visible, with Scer\GAL4bbg-C96 kek1d03841 visible, with Scer\GAL4GMR.PF kek1Scer\UAS.cGa kek1Scer\UAS.T:Avic\GFP visible (with kek1RA5) kek1RM2 visible (with kek1RM2) kek1RA5 Phenotype manifest in Allele border follicle cell, with Scer\GAL4slbo.2.6 kek1Scer\UAS.cGa chorion, with Scer\GAL4CY2 kek1Scer\UAS.T:Avic\GFP kek1Scer\UAS.cGa chorion, with Scer\GAL4GMR.PF kek1118.Scer\UAS.T:Avic\GFP kek1137.Scer\UAS.T:Avic\GFP kek1176V.Scer\UAS.T:Avic\GFP kek165.Scer\UAS.T:Avic\GFP kek196.Scer\UAS.T:Avic\GFP chorion, with Scer\GAL4T155 kek1Scer\UAS.cGa chorion | dorsal, with Scer\GAL4CY2 kek1ΔIC+T.Scer\UAS.T:Avic\GFP kek1ΔIC.Scer\UAS.T:Avic\GFP kek1Scer\UAS.T:Avic\GFP dorsal appendage kek1RA5 kek1RM2 dorsal appendage, with Scer\GAL4CY2 kek1ΔIg.Scer\UAS kek1ΔNT.Scer\UAS kek1extra.Scer\UAS dorsal appendage, with Scer\GAL4nos.UTR.T:Hsim\VP16 kek1Scer\UAS.P\T.cGa dorsal appendage, with Scer\GAL4T155 kek1Scer\UAS.cGa kek1extra.Scer\UAS dorsal appendage (with kek1RA5) kek1RM2 dorsal appendage (with kek1RM2) kek1RA5 dorsal appendage | maternal effect (with kek1RA5) kek1RM2 dorsal appendage | maternal effect (with kek1RM2) kek1RA5 egg kek1RA5 kek1RM2 egg, with Scer\GAL4CY2 kek1ΔIg.Scer\UAS kek1ΔNT.Scer\UAS egg, with Scer\GAL4nos.UTR.T:Hsim\VP16 kek1Scer\UAS.P\T.cGa egg, with Scer\GAL4T155 kek1Scer\UAS.cGa embryo, with Scer\GAL4CY2 kek1extra.Scer\UAS embryo, with Scer\GAL4T155 kek1extra.Scer\UAS embryonic/larval neuroblast | larval stage kek1GD28 eye, with Scer\GAL4GMR.PF kek1118.Scer\UAS.T:Avic\GFP kek1137.Scer\UAS.T:Avic\GFP kek1176V.Scer\UAS.T:Avic\GFP kek165.Scer\UAS.T:Avic\GFP kek196.Scer\UAS.T:Avic\GFP kek1Scer\UAS.T:Avic\GFP kek1Scer\UAS.cGa ganglion mother cell | larval stage kek1GD28 ommatidium, with Scer\GAL4GMR.PF kek1Scer\UAS.T:Avic\GFP kek1Scer\UAS.cGa photoreceptor cell, with Scer\GAL4GMR.PF kek1Scer\UAS.cGa posterior crossvein, with Scer\GAL4en-e16E kek1Scer\UAS.cGa wing, with Scer\GAL471B kek1Scer\UAS.cGa wing, with Scer\GAL4bbg-C96 kek1d03841 wing vein, with Scer\GAL471B kek1Scer\UAS.cGa wing vein, with Scer\GAL4Bx-MS1096 kek1ΔIg.Scer\UAS kek1ΔNT.Scer\UAS kek1Scer\UAS.cGa wing vein | ectopic (with kek1RA5) kek1RM2 wing vein | ectopic (with kek1RM2) kek1RA5 wing vein L4, with Scer\GAL4en-e16E kek1Scer\UAS.cGa kek1extra.Scer\UAS wing vein L5, with Scer\GAL4en-e16E kek1Scer\UAS.cGa
Classical Alleles ( 14 )
For All Classical Alleles Show
Allele of kek1 Class Mutagen Stocks Known lesion kek1k07332 P-element activity 2 -- kek101433 P-element activity 1 -- kek1d03841 1 -- kek115A6 P-element activity 0 Yes kek13K2S X ray 0 Yes kek13K2X X ray 0 Yes kek1AN296 P-element activity 0 -- kek1DG23812 P-element activity 0 -- kek1EP655 0 -- kek1RA5 Delta2-3 0 Yes kek1RJ22 Delta2-3 0 Yes kek1RK9 Delta2-3 0 Yes kek1RM2 Delta2-3 0 Yes kek1unspecified 0 --
Alleles Carried on Transgenic Constructs ( 28 )
For All Alleles Carried on Transgenic Constructs Show
Allele of kek1 Class Mutagen Stocks Known lesion kek1GD14381 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes kek1GD2524 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes kek1GD28 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes kek1KK105604 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes kek1118.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek1137.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek1176V.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek1191.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek153B.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek165.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek17C.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek182.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek182A.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek196.Scer\UAS.T:Avic\GFP ethyl methanesulfonate in vitro construct 0 Yes kek1extra.Scer\UAS in vitro construct - regulatory fusion 0 Yes kek1intra.Scer\UAS.T:Uuuu\Myr4 in vitro construct - regulatory fusion 0 Yes kek1Scer\UAS.cGa in vitro construct - regulatory fusion 0 Yes kek1Scer\UAS.P\T.cGa in vitro construct - regulatory fusion 0 Yes kek1Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes kek1Scer\UAS.T:SV5\V5 in vitro construct - regulatory fusion 0 Yes kek1sec.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes kek1ΔIC+T.Scer\UAS.T:Avic\GFP in vitro construct - coding region fusion 0 Yes kek1ΔIC.Scer\UAS.T:Avic\GFP in vitro construct - deletion 0 Yes kek1ΔIg.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes kek1ΔLLR.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes kek1ΔNT.LLR.Ig.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes kek1ΔNT.LLR.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes kek1ΔNT.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Exel6034 (Ryder, 2004.4.26) Df(2L)kek13K2X (Musacchio and Perrimon, 1996) Df(2L)kek1RA5 (Musacchio and Perrimon, 1996) Df(2L)Prl (Musacchio and Perrimon, 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD28} NA P{GD2524} NA P{GD14381} NA P{KK105604} NA P{UAS-kek1.7C.GFP} NA P{UAS-kek1.53B.GFP} NA P{UAS-kek1.65.GFP} NA P{UAS-kek1.82.GFP} NA P{UAS-kek1.82A.GFP} NA P{UAS-kek1.96.GFP} NA P{UAS-kek1.118.GFP} NA P{UAS-kek1.137.GFP} NA P{UAS-kek1.176V.GFP} NA P{UAS-kek1.191.GFP} NA P{UAS-kek1.extra} NA P{UAS-kek1.G} NA P{UAS-kek1.GFP} NA P{UAS-kek1.intra} NA P{UAS-kek1.sec} NA P{UAS-kek1.V5} NA P{UAS-kek1.ΔIC+T.GFP} NA P{UAS-kek1.ΔIg} NA P{UAS-kek1.ΔLLR} NA P{UAS-kek1.ΔNT.LLR.Ig} NA P{UAS-kek1.ΔNT.LLR} NA P{UAS-kek1.ΔNT} NA P{UAS-kek1ΔIC.GFP} NA P{UASp-kek1.G} NA characterization construct P{UAS-kek1::Egfr.G} NA P{UAS-kek1::Egfr.KEg} NA P{UAS-kek1::Egfr.KEG} NA P{UAS-kek1::Egfr.KEgG} NA P{UAS-kek1::Egfr.KEkG} NA P{UAS-kek1::Egfr.KEΔCG} NA P{UAS-kek1::Egfr.KEΔKG} NA P{UAS-kek1::kek2.LGFP} NA P{UAS-kek1::kek2.LIGFP} NA P{UAS-kek1::kek2.LITGFP} NA P{UAS-kek1.ΔIg::Egfr.G} NA P{UAS-kek1.ΔLRR::Egfr} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}EP509 NA P{EP}EP588 NA P{EP}EP849 NA P{EP}kek1EP655 NA P{GSV1}C762C762 NA P{Mae-UAS.6.11}B0911B0911 NA P{XP}kek1d03841 NA insertion of enhancer trap P{lacW}kek1AN296 No P{lacW}kek1k07332 No P{lArB}kek115A6 No P{PZ}kek101433 No miscellaneous insertions P{GT1}BG01891BG01891 NA P{PTT-GB}CB02190 NA P{wHy}kek1DG23812 NA
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
epidermal growth factor receptor binding inferred from physical interaction with Egfr (Ghiglione et al., 1999, Alvarado et al., 2004) receptor antagonist activity inferred from direct assay (Alvarado et al., 2004)
Biological Process
CV term Evidence References
cytokinesis inferred from mutant phenotype (Kiger et al., 2003) negative regulation of epidermal growth factor-activated receptor activity inferred from direct assay (Alvarado et al., 2004) negative regulation of epidermal growth factor receptor signaling pathway inferred from genetic interaction with Egfr AND inferred from genetic interaction with grk AND inferred from mutant phenotype (Ghiglione et al., 1999) inferred from genetic interaction with Egfr AND inferred from mutant phenotype (Alvarado et al., 2004) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011) oogenesis inferred from mutant phenotype (Ghiglione et al., 1999)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
negative regulation of epidermal growth factor-activated receptor activity traceable author statement (Shilo, 2003) negative regulation of epidermal growth factor receptor signaling pathway traceable author statement (van Eeden and St. Johnston, 1999)
Cellular Component
CV term Evidence References
integral to membrane traceable author statement (Shilo, 2003) integral to plasma membrane inferred from sequence model (Musacchio and Perrimon, 1996, Derheimer et al., 2004)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
kek1 - Tsp29Fb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions kek1 allele Gene References kek1RA5 Egfr (Ghiglione et al., 1999, Alvarado et al., 2004, Alvarado et al., 2004) grk (Ghiglione et al., 1999) Nrt (Speicher et al., 1998) kek1RM2 Egfr (Ghiglione et al., 1999, Alvarado et al., 2004, Alvarado et al., 2004) grk (Ghiglione et al., 1999) Nrt (Speicher et al., 1998) kek1Scer\UAS.cGa ed (Bai et al., 2001) Egfr (Alvarado et al., 2004) grk (Alvarado et al., 2004) kek1Scer\UAS.P\T.cGa grk (Ghiglione et al., 2003) unspecified Egfr (Derheimer et al., 2004) L (Singh et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60732 DroID - A comprehensive database of gene and protein interactions. • FBgn0015399 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34688 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8T3J2
Orthologs
OrthoDB Orthologs (31) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6CNR9K Dipteran inclusive ortholog search EOG6D7ZDR Insect inclusive ortholog search EOG6CC2W8 Arthropod inclusive ortholog search EOG6N02VZ Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6CNR9K)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\kek1     Drosophila simulans   Dsim\GD22095 Y   Drosophila sechellia   Dsec\GM25965 Y   Drosophila erecta   Dere\GG10236 Y   Drosophila yakuba   Dyak\GE12011 Y   Drosophila ananassae   Dana\GF15653 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA11531 Y   Drosophila persimilis   Dper\GL10073 Y   Drosophila willistoni   Dwil\GK14817 Y   Drosophila virilis   Dvir\GJ11119 Y   Drosophila mojavensis   Dmoj\GI21281 Y   Drosophila grimshawi   Dgri\GH10356 Y
Orthologs in non-Drosophila Dipterans (EOG6D7ZDR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001368   Anopheles gambiae Malaria mosquito  Agam\AGAP009136   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015995
Orthologs in non-Dipteran Insects (EOG6CC2W8)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\kek1   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007349   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH23160   Atta cephalotes Leafcutter ant  Acep\ACEP24391   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14546   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15118   Linepithema humile Argentine ant  Lhum\LH15512   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25506   Acyrthosiphon pisum Pea aphid  Apim\ACYPI007861   Bombyx mori Silkmoth  Bmor\BGIBMGA010659   Tribolium castaneum Red flour beetle  Tcas\kek-1
Orthologs in non-Insect Arthropods (EOG6N02VZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_318592 Y Daphnia pulex Water flea  Dpux\mdh Y Ixodes scapularis Deer tick  Isca\ISCW018003 Y Ixodes scapularis Deer tick  Isca\ISCW018006 Y Ixodes scapularis Deer tick  Isca\ISCW020118 Y
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22095 Drosophila sechellia Dsec\GM25965 Drosophila erecta Dere\GG10236 Drosophila yakuba Dyak\GE12011 Drosophila ananassae Dana\GF15653 Drosophila pseudoobscura pseudoobscura Dpse\GA11531 Drosophila persimilis Dper\GL10073 Drosophila willistoni Dwil\GK14817 Drosophila virilis Dvir\GJ11119 Drosophila mojavensis Dmoj\GI21281 Drosophila grimshawi Dgri\GH10356
Stocks & Reagents
Stocks Listed in FlyBase ( 8 )
Bloomington	10669 y1 w67c23; P{lacW}kek1k07332, l(2)k07332k07332/CyO 11053 l(2)gl01433 P{PZ}kek101433 l(2)36Ba01433 cn1/CyO; ry506
Harvard	- P{XP}kek1d03841
Kyoto	111214 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}kek1k07332 P{neoFRT}40A/CyO y+
VDRC	v101166 P{KK105604}VIE-260B v43521 w1118; P{GD28}v43521 v4761 w1118; P{GD2524}v4761 v36252 w1118; P{GD14381}v36252
Genomic Clones ( 2 )
	BACR11M13 BACR17H02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 68 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GM02380 SD01674
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GH24240 GH25027 GH26720 LP05970 LP15321 LP16544 SD09468 SD11245 SD11702 SD13230 SD15692 SD16977 SD21540 SD23411
Other clones	12951 13303 15359 16127 29792 30092 36577 47223 53727 58151 65832 97870 100130 103256 121691 164880 190642 522206 527037 572890 AT01130 CK01.2D.H2 CK01.76C.D10 CK01.88A.C10 CK01.102A.A8 CK01.117C.F6 EK059935 EK072201 EK072241 EK086036 EK114452 EK115413 EK126004 EK127814 EK131914 EK137951 EK147708 EK178333 EK193163 EK220312 EK222302 EK269530 EK276208 EK290643 EK292658 EN01547 EN07151 EN17320 FGM207F01 FGM311G04 FGM319C07 GH23277
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015399 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34688
Antibody Information
Other Information
Discoverer
Etymology
	kek1 and kek2 represent the union of two superfamilies within one molecule (leucine-rich repeat and immunoglobulin superfamily), so they have been named "kekkon", the Japanese word for marriage. (Musacchio and Perrimon, 1996)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: kek1 BEST:GM02380 (FlyBase, 1996-)
Additional comments
Other Comments
	The leucine rich repeats of kek1 protein are sufficient for binding Egfr protein, however, inhibition of Egfr protein by kek1 protein in vivo requires the kek1 juxta/transmembrane region. kek1 protein is not a phosphorylation substrate for the Egfr protein in vivo. (Alvarado et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167: binucleate cells. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) kek1 acts as an inhibitor of Egfr. (Ghiglione et al., 1999) Original 'unstrung' phenotype was in fact due to deletion of more than one gene, one of which has been shown to be kek1. (Perrimon, 1998.8.10) kek1 is a sensitive marker for Egfr activity in follicle cells. (Sapir et al., 1998) kek1 expression is regulated in the developing epithelia in addition to the CNS. The absence of kek1 gene product causes no overt phenotype. Results suggest kek1 and kek2, or additional genes, may overlap in function in the assembly of the CNS. (Musacchio and Perrimon, 1996) Mutants of kek1 exhibit missing intersegmental longitudinal tracts. (Musacchio and Perrimon, 1991)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078835 RefSeq (Proteins) • NP_523559 DDBJ / EMBL / GenBank • AA695190 AAC47404 AAF53225 AAM11421 ABA86436 AI530905 AQ025829 AW944198 AY095093 DQ138830 G00461 U42767 Entrez Gene - A searchable database of RefSeq genes. • 34688 UniProtKB/TrEMBL • P91643 Q2XY32 Q8T3J2 Q9VK54
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Immunoglobulin subtype 2 (IPR003598) Cysteine-rich flanking region, C-terminal (IPR000483) Leucine-rich repeat (IPR001611) Immunoglobulin I-set (IPR013098) Immunoglobulin-like fold (IPR013783) Leucine-rich repeat, typical subtype (IPR003591) Immunoglobulin-like domain (IPR007110)
Linkouts
	BioGRID - A database of protein and genetic interactions • 60732 DroID - A comprehensive database of gene and protein interactions. • FBgn0015399 DRSC - Results from RNAi screens. • FBgn0015399 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015399 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG12283-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015399 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34688 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/kekkon1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34688 modMine - Data generated by the modENCODE project. • FBgn0015399 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q8T3J2
Synonyms & Secondary IDs ( 28 )
Reported As
Symbol Synonym	BEST:GM02380   CG12283 (Molnar et al., 2006, Grieder et al., 2007, Mosallanejad et al., 2010, Keleman et al., 2009.8.5) CT18186 (Hynes and Zhao, 2000, Hynes and Zhao, 2000) Dmkek1 (Derheimer et al., 2004) GM02380 (Spradling et al., 1999) Kek (Freeman, 2002) kek (Guichet et al., 2001, Peri and Roth, 2000, Mathieu et al., 2007, Peretz et al., 2007, Shravage et al., 2007, Sun et al., 2011, Firth and Baker, 2007, Xia et al., 2008, Li et al., 2009, Yan et al., 2011, Ferguson et al., 2012) kek-1 (Singh et al., 2005, Goff and Morisato, 2000, Goode et al., 1996, Molnar et al., 2006, Grieder et al., 2007) Kek-1 (de Lorenzo et al., 2003, de Lorenzo et al., 2001) KEK1 (Alvarado and Duffy, 2002) Kek1 (Bai et al., 2001, Huwe et al., 2000, Musacchio and Perrimon, 1996) kek1 (Terry et al., 2006, Atkey et al., 2006, Pallavi et al., 2006, Layalle et al., 2004, Buszczak et al., 2007, Stephan et al., 2008, Kankel et al., 2007, Sandmann et al., 2006, Fan and Bergmann, 2008, Kucherenko et al., 2008, Bartkuhn et al., 2009, Zartman et al., 2009, Zeitlinger et al., 2007, Hall et al., 2004, Kitadate and Kobayashi, 2010, Cherbas et al., 2011) kekon (Shilo, 2003, Spencer and Cagan, 2002, Sapir et al., 1998) l(2)01433 (Spradling et al., 1999) n(2)k07332 (Spradling, 1999.12.9) NB1 (Musacchio and Perrimon, 1996) SD01674 (Herold et al., 2003) uns (Musacchio and Perrimon, 1991, )
Name Synonym	Kekkon (Yakoby et al., 2005, Depraetere, 2000) kekkon (Tanentzapf and Tepass, 2003, Bogdan and Klaembt, 2001, van Buskirk and Schupbach, 1999, Pallavi et al., 2006, Motola and Neuman-Silberberg, 2004, Bernardi et al., 2007, Firth and Baker, 2007, Xia et al., 2008, Li et al., 2009, Yan et al., 2011, Goentoro et al., 2006) Kekkon-1 (Vogel et al., 2003, Firth and Baker, 2003) kekkon-1 (van Eeden and St. Johnston, 1999, Terry et al., 2006, Kankel et al., 2007, Zartman et al., 2009, Hall et al., 2004) kekkon1 (Perrimon, 1998.8.10, Chen et al., 2007) Kekkon 1 (Firth and Baker, 2003) Kekkon1 (Stephan et al., 2008, Goentoro et al., 2006) kekkonI (Brendza et al., 2002) Kekon-1 (Silverman et al., 2003) unstrung (Musacchio and Perrimon, 1991, )
Secondary FlyBase IDs
	FBgn0005766 FBgn0028957
References ( 121 )
Generate a list of	All references relating to this gene ( 121 )
List References by type	Research paper  ( 63 ) Supplementary material  ( 9 ) Review  ( 16 ) Personal communication to FlyBase  ( 11 ) Abstract  ( 14 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 4 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Ferguson et al., 2012, J. Cell Sci. 125(6): 1407--1419 Modulation of gurken translation by insulin and TOR signaling in Drosophila. [FBrf0218097] Cherbas et al., 2011, Genome Res. 21(2): 301--314 The transcriptional diversity of 25 Drosophila cell lines. [FBrf0213077] Sun et al., 2011, Development 138(10): 1991--2001 Regulation of somatic myosin activity by Protein Phosphatase 1{beta} controls Drosophila oocyte polarization. [FBrf0213586] Yan et al., 2011, Development 138(9): 1697--1703 Drosophila PI4KIIIalpha is required in follicle cells for oocyte polarization and Hippo signaling. [FBrf0213488] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...