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Gene: Dmel\mbc

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General Information
Symbol
Dmel\mbc
Species
D. melanogaster
Name
myoblast city
Annotation Symbol
CG10379
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015513
Gene Model Status
Current
Stock Availability
23 publicly available
Gene Snapshot
Myoblast city is an unconventional bipartite GEF that, in combination with Ced-12, activates the Rac1 monomeric GTPase. It contributes to myoblast fusion, border cell migration and dorsal closure. [Date last reviewed: 2017-03-02]
Other Summaries
Also Known As
Dock180, Su(rac)1
Key Links
Genomic Location
Cytogenetic map
95B5-95B7
Sequence location
3R:23,781,765..23,801,634 [+]
Recombination map
3-80
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI Genome Data Viewer
UCSC Genome Browser
Ensembl Genome Browser
PopFly Genome Browser
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
-
Protein Domains/Motifs
InterPro
SH3 domain; Dedicator of cytokinesis, C-terminal; Dedicator of cytokinesis; DHR-1 domain; DHR-2 domain; DOCK1 homologue; Dedicator of cytokinesis, N-terminal domain; C2 domain superfamily; SH3-like domain superfamily
Molecular Function (see GO section for details)
Experimental Evidence
protein binding; Rac guanyl-nucleotide exchange factor activity
Predictions / Assertions
-
Summaries
Gene Group Membership
RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS -
RhoGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Rho family GTPases. They include Dbl-homology domain-containing proteins (the largest family of RhoGEFs) and CZH2 domain/Dock family GEFs. (Adapted from FBrf0190957).
Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components -
PDGF/VEGF-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependant manner. (Adapted from FBrf0222697 and FBrf0221727).
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\mbc or the JBrowse view of Dmel\mbc for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
mbc-RA
FBtr0084459
NM_057796
7351
1970
mbc-RB
FBtr0304729
NM_001260338
7465
2008
Additional Transcript Data and Comments
Reported size (kB)
7.5 (northern blot)
(Erickson et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
mbc-PA
FBpp0083850
226.5
1970
7.25
NP_477144
AAF56191
mbc-PB
FBpp0293271
230.7
2008
7.45
NP_001247267
AFH06585
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)
1970 (aa); 226 (kD predicted)
(Erickson et al., 1997)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mbc using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (22 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
protein binding
inferred from physical interaction with FLYBASE:rols; FB:FBgn0041096
(Chen and Olson, 2001)
Rac guanyl-nucleotide exchange factor activity
inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333
(Haralalka et al., 2011)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (17 terms)
Terms Based on Experimental Evidence (16 terms)
CV Term
Evidence
References
actin cytoskeleton organization
inferred from mutant phenotype
(Haralalka et al., 2011, Richardson et al., 2007)
activation of GTPase activity
inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333
(Haralalka et al., 2011)
(Geisbrecht et al., 2008)
inferred from genetic interaction with FLYBASE:Rac1; FB:FBgn0010333, FLYBASE:Ced-12; FB:FBgn0032409
(Geisbrecht et al., 2008)
anterior Malpighian tubule development
inferred from mutant phenotype
(Pütz et al., 2005)
border follicle cell migration
inferred from mutant phenotype
(Geisbrecht et al., 2008)
cell competition in a multicellular organism
inferred from mutant phenotype
(Li and Baker, 2007)
central nervous system development
inferred from mutant phenotype
(Biersmith et al., 2011)
cytoskeleton organization
inferred from mutant phenotype
(Erickson et al., 1997)
dorsal closure
inferred from mutant phenotype
(Erickson et al., 1997)
imaginal disc fusion, thorax closure
inferred from genetic interaction with FLYBASE:Pvr; FB:FBgn0032006
(Ishimaru et al., 2004)
larval visceral muscle development
inferred from mutant phenotype
(Klapper et al., 2002)
muscle attachment
inferred from genetic interaction with FLYBASE:msk; FB:FBgn0026252
(Liu et al., 2013)
inferred from genetic interaction with FLYBASE:Ced-12; FB:FBgn0032409
(Liu et al., 2013)
myoblast fusion
inferred from mutant phenotype
(Haralalka et al., 2011, Shelton et al., 2009, Richardson et al., 2007, Balagopalan et al., 2006, Estrada et al., 2006, Menon et al., 2005, Rushton et al., 1995)
positive regulation of border follicle cell migration
inferred from mutant phenotype
(Duchek et al., 2001)
positive regulation of JNK cascade
inferred from direct assay
(Ishimaru et al., 2004)
positive regulation of Rac protein signal transduction
inferred from direct assay
(Ishimaru et al., 2004)
vascular endothelial growth factor receptor signaling pathway
inferred from mutant phenotype
(Ishimaru et al., 2004, Duchek et al., 2001)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
cell morphogenesis
traceable author statement
(Noselli and Agnes, 1999)
dorsal closure
traceable author statement
(Harden, 2002, Noselli and Agnes, 1999)
myoblast fusion
traceable author statement
(Dworak and Sink, 2002)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
cytoplasm
inferred from direct assay
(Erickson et al., 1997)
extrinsic component of plasma membrane
inferred from direct assay
(Haralalka et al., 2011)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
ruffle
traceable author statement
(Noselli and Agnes, 1999)
Expression Data
Transcript Expression
expression microarray
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
indirect flight muscle
(Katti et al., 2017)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
pole plasm

Comment: maternally deposited

(Fisher et al., 2012)
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 7 -- 8
anterior endoderm anlage
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
anterior endoderm
(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
posterior endoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 11 -- 12
anterior midgut primordium
(Fisher et al., 2012)
dorsal pharyngeal muscle primordium
(Fisher et al., 2012)
longitudinal visceral muscle primordium
(Fisher et al., 2012)
posterior midgut primordium
(Fisher et al., 2012)
somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
embryonic stage 12
ectoderm | striped
(Erickson et al., 1997)
embryonic stage 13 -- 16
dorsal pharyngeal muscle
(Fisher et al., 2012)
embryonic/larval muscle system
(Fisher et al., 2012)
embryonic/larval somatic muscle
(Fisher et al., 2012)
embryonic/larval visceral muscle
(Fisher et al., 2012)
longitudinal visceral muscle fiber
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Erickson et al., 1997)
embryonic stage 1 -- 9

Comment: reference states 0-4 hr AEL

(Erickson et al., 1997)
embryonic stage 4
pole cell
(Erickson et al., 1997)
embryonic stage 5
organism | lateral
(Erickson et al., 1997)
embryonic stage 7 -- 12
ectoderm
(Erickson et al., 1997)
embryonic stage 12
endoderm
(Erickson et al., 1997)
embryonic stage 12 -- 14
somatic mesoderm
(Erickson et al., 1997)
embryonic stage 12 -- 17
visceral mesoderm
(Erickson et al., 1997)
embryonic stage 14
embryonic/larval visceral muscle
(Erickson et al., 1997)
embryonic stage 16
embryonic/larval dorsal vessel
(Erickson et al., 1997)
pupal stage
(Erickson et al., 1997)
adult stage | female
(Erickson et al., 1997)
adult stage | male
(Erickson et al., 1997)
RT-PCR
Stage
Tissue/Position (including subcellular localization)
Reference
pupal stage P6
dorsal medial indirect flight muscle

Comment: 24 hr APF

(Bryantsev et al., 2012)
thoracic adult muscle precursor cell
(Bryantsev et al., 2012)
Additional Descriptive Data
Both the cardial and pericardial cells of the dorsal vessel express mbc transcript. No mbc transcript is detected in mature muscles.
(Erickson et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 5
pole cell
(Erickson et al., 1997)
embryonic stage 12
endoderm
(Erickson et al., 1997)
myoblast
(Erickson et al., 1997)
ectoderm
(Erickson et al., 1997)
embryonic stage 14
embryonic/larval visceral muscle
(Erickson et al., 1997)
embryonic stage 15 -- late third instar larval stage
muscle attachment site
(Liu et al., 2013)
embryonic stage 16
embryonic/larval dorsal vessel
(Erickson et al., 1997)
larval stage
embryonic/larval hypodermal muscle
• Z disc
(Liu et al., 2013)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
mbc protein expression correlates approximately with mbc transcript expression. Although mbc transcript is not detected in mature muscles, mbc protein is detected at low levels in mature muscles. Colocalization studies with Mef2 protein indicate that mbc protein is present in ectodermal and endodermal germ layers, with ectodermal expression being restricted to the epidermal layer, and that mbc is expressed in myoblasts.
(Erickson et al., 1997)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
cytoplasm
inferred from direct assay
(Erickson et al., 1997)
extrinsic component of plasma membrane
inferred from direct assay
(Haralalka et al., 2011)
Expression Deduced from Reporters
Reporter: PBac{602.P.SVS-1}mbcCPTI000332
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mbc in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 43 )
For All Classical and Insertion Alleles Show
 
Allele of mbc
Class
Mutagen
Associated Insertion
Stocks
Known lesion
mbcD11.2
loss of function allele
1
Yes
mbcC1
amorphic allele - genetic evidence, loss of function allele
1
--
mbcunspecified
    0
    --
    mbcC2
    amorphic allele - genetic evidence, loss of function allele
    1
    --
    mbcEY01437
    1
    Yes
    mbcC3
    amorphic allele - genetic evidence
    1
    --
    mbcCPTI000332
    1
    Yes
    mbc2.35
    0
    --
    mbcF6.4
    loss of function allele
    0
    Yes
    mbcF12.7
    0
    Yes
    mbcTT261
    0
    Yes
    mbcc04167
    1
    Yes
    mbcc05056
    1
    Yes
    mbcc05087
    1
    Yes
    mbcc05391
    1
    Yes
    mbcCB-0487-3
      1
      Yes
      mbcCPTI001082
      1
      Yes
      mbcf06027
      1
      Yes
      mbcMI06051
        1
        Yes
        mbcMI06051-TG4.1
        1
        Yes
        mbcNP5474
          1
          Yes
          mbcS4
          amorphic allele - genetic evidence
          0
          --
          mbc1.63
          0
          --
          mbc3.5
          0
          --
          mbc4.25
          0
          --
          mbcAC177
          0
          Yes
          mbcC6.1
          0
          Yes
          mbcC9.2
          0
          Yes
          mbcC14.3
          0
          Yes
          mbcc03965
          0
          Yes
          mbcCB04603
          0
          Yes
          mbcE26.2
          0
          Yes
          mbcE33.1
          0
          Yes
          mbcEP3135
          0
          Yes
          mbcEPg36390
          0
          Yes
          mbcF4.4
          0
          Yes
          mbcF6.7
          0
          Yes
          mbcI6.6
          0
          Yes
          mbcJ342
          0
          Yes
          mbcO146
          0
          Yes
          mbcZZ1
          0
          Yes
          mbcZZ260
          0
          Yes
          mbczz351
            0
            --
            Other relevant insertions
            Transgenic Constructs ( 21 )
            For All Alleles Carried on Transgenic Constructs Show
            Transgenic constructs containing/affecting coding region of mbc
            Allele of mbc
            Mutagen
            Associated Transgenic Construct
            Stocks
            mbcGD6965
            1
            mbcdsRNA.UAS
            0
            mbcUAS.cBa
            0
            mbcUAS.Tag:HA
            0
            mbcHMC03172
            1
            mbcHMS00346
            1
            mbcNPxxP.Δ1807.UAS.Tag:HA
            0
            mbcGLC01361
            1
            mbcHMC03198
            1
            mbcHMS00604
            1
            mbcVSH330278
            1
            mbcCBS.UAS
            0
            mbcDockerF6.4.UAS.Tag:HA
            0
            mbcNIG.10379R
            0
            mbcNPxxP.UAS.Tag:HA
            0
            mbcNPxxP.ΔDHR1.Δ1807.UAS.Tag:HA
            0
            mbcSH3W47K.UAS.Tag:HA
            0
            mbcUAS.Tag:MYC,Tag:HA
            0
            mbcΔ1807.UAS.Tag:HA
            0
            mbcΔDHR1.UAS.Tag:HA
            0
            mbcΔPRM.UAS.Tag:HA
            0
            Transgenic constructs containing regulatory region of mbc
            vital-reporter construct
            Name
            Expression Data
            P{mbc-GFP.64}
            No
            Deletions and Duplications ( 16 )
            Summary of Phenotypes
            For more details about a specific phenotype click on the relevant allele symbol.
            Lethality
            Allele
            lethal
            lethal, with Scer\GAL4Act5C.PI
            lethal | recessive
            lethal - all die before end of embryonic stage | recessive
            lethal - all die during P-stage, with Scer\GAL4Mef2.PR
            partially lethal - majority die, with Scer\GAL4pnr-MD237
            viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
            viable, with Scer\GAL4tin.CΔ4
            Other Phenotypes
            Allele
            endocytosis defective, with Scer\GAL4repo, Scer\GAL80ts.αTub84B
            neuroanatomy defective, with Scer\GAL4alrm.PD
            neuroanatomy defective | embryonic stage
            some die during embryonic stage | recessive
            some die during pupal stage, with Scer\GAL4pnr-MD237
            visible, with Scer\GAL4pnr-MD237
            Phenotype manifest in
            Allele
            A1-7 dorsal acute muscle 1
            A1-7 dorsal acute muscle 1 | embryonic stage 15
            axon & ventral nerve cord
            border follicle cell, with Scer\GAL4slbo.2.6
            border follicle cell | somatic clone
            chaeta | supernumerary, with Scer\GAL4pnr-MD237
            circular visceral muscle fiber
            circular visceral muscle primordium
            commissure
            dorsal pharyngeal muscle
            embryonic/larval anterior Malpighian tubule
            embryonic/larval Malpighian tubule
            embryonic/larval midgut longitudinal visceral muscle | embryonic stage (with mbcC1)
            embryonic/larval midgut longitudinal visceral muscle | embryonic stage (with mbcD11.2)
            embryonic/larval muscle system
            embryonic/larval somatic muscle
            embryonic dorsal epidermis
            embryonic Malpighian tubule
            embryonic midgut constriction
            embryonic midgut constriction 1 | embryonic stage (with mbcC1)
            embryonic midgut constriction 1 | embryonic stage (with mbcD11.2)
            embryonic myoblast
            embryonic myoblast | germline clone
            embryonic visceral muscle
            fusion competent cell | embryonic stage
            gamma Kenyon cell, with Scer\GAL4alrm.PD
            glial cell, with Scer\GAL4repo, Scer\GAL80ts.αTub84B
            lateral longitudinal fascicle
            longitudinal connective
            longitudinal muscle
            longitudinal visceral muscle primordium (with mbcC1)
            longitudinal visceral muscle primordium (with mbcD11.2)
            Malpighian tubule Type II cell
            mesothoracic tergum, with Scer\GAL4pnr-MD237
            muscle founder cell | embryonic stage
            myoblast
            presumptive embryonic/larval muscle system
            presumptive embryonic/larval muscle system | germline clone
            reticular neuropil associated glial cell, with Scer\GAL4alrm.PD
            thorax, with Scer\GAL4pnr-MD237
            vesicle, with Scer\GAL4repo, Scer\GAL80ts.αTub84B
            Orthologs
            Downloads
            Download All DIOPT Orthologs
            Download All OrthoDB Orthologs
            Human Orthologs (via DIOPT v7.1)
            Homo sapiens (Human) (5)
            Species\Gene Symbol
            Score
            Best Score
            Best Reverse Score
            Source
            Compara
            eggNOG
            Hieranoid
            Homologene
            Inparanoid
            Isobase
            OMA
            OrthoDB
            OrthoFinder
            OrthoInspector
            orthoMCL
            Panther
            Phylome
            RoundUp
            TreeFam
            ZFIN
            Alignment
            Complementation?
            Transgene?
            Hsap\DOCK1
            12 of 15
            Yes
            Yes
            Hsap\DOCK2
            12 of 15
            Yes
            Yes
            Hsap\DOCK5
            11 of 15
            No
            Yes
            Hsap\DOCK3
            5 of 15
            No
            No
            Hsap\DOCK4
            3 of 15
            No
            No
            Model Organism Orthologs (via DIOPT v7.1)
            Mus musculus (laboratory mouse) (5)
            Species\Gene Symbol
            Score
            Best Score
            Best Reverse Score
            Source
            Compara
            eggNOG
            Hieranoid
            Homologene
            Inparanoid
            Isobase
            OMA
            OrthoDB
            OrthoFinder
            OrthoInspector
            orthoMCL
            Panther
            Phylome
            RoundUp
            TreeFam
            ZFIN
            Alignment
            Complementation?
            Transgene?
            Mmus\Dock1
            13 of 15
            Yes
            Yes
            Mmus\Dock2
            11 of 15
            No
            Yes
            Mmus\Dock5
            11 of 15
            No
            Yes
            Mmus\Dock3
            5 of 15
            No
            No
            Mmus\Dock4
            5 of 15
            No
            No
            Rattus norvegicus (Norway rat) (5)
            Rnor\Dock1
            12 of 13
            Yes
            Yes
            Rnor\Dock5
            9 of 13
            No
            Yes
            Rnor\Dock3
            4 of 13
            No
            No
            Rnor\Dock4
            3 of 13
            No
            No
            Rnor\Dock2
            2 of 13
            No
            Yes
            Xenopus tropicalis (Western clawed frog) (6)
            Xtro\dock1
            5 of 12
            Yes
            Yes
            Xtro\dock2
            3 of 12
            No
            Yes
            Xtro\dock3
            2 of 12
            No
            No
            Xtro\dock4
            2 of 12
            No
            No
            Xtro\dock5
            1 of 12
            No
            Yes
            Xtro\LOC101730451
            1 of 12
            No
            Yes
            Danio rerio (Zebrafish) (6)
            Drer\dock1
            7 of 15
            Yes
            Yes
            Drer\dock5
            7 of 15
            Yes
            Yes
            Drer\dock4b
            5 of 15
            No
            No
            Drer\dock3
            3 of 15
            No
            No
            Drer\si:dkey-81e3.1
            3 of 15
            No
            No
            Drer\dock2-like
            1 of 15
            No
            Yes
            Caenorhabditis elegans (Nematode, roundworm) (1)
            Cele\ced-5
            15 of 15
            Yes
            Yes
            Arabidopsis thaliana (thale-cress) (0)
            No orthologs reported.
            Saccharomyces cerevisiae (Brewer's yeast) (1)
            Scer\DCK1
            7 of 15
            Yes
            Yes
            Schizosaccharomyces pombe (Fission yeast) (0)
            No orthologs reported.
            Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900A6 )
            Organism
            Common Name
            Gene
            AAA Syntenic Ortholog
            Multiple Dmel Genes in this Orthologous Group
            Drosophila melanogaster
            fruit fly
            Dmel\mbc
            Drosophila suzukii
            Spotted wing Drosophila
            Dsuz\DS10_00011949
            Drosophila simulans
            Dsim\GD21040
            Drosophila sechellia
            Dsec\GM26532
            Drosophila erecta
            Dere\GG11230
            Drosophila yakuba
            Dyak\GE10396
            Drosophila ananassae
            Dana\GF16263
            Drosophila pseudoobscura pseudoobscura
            Dpse\GA10288
            Drosophila persimilis
            Dper\GL23263
            Drosophila willistoni
            Dwil\GK11109
            Drosophila virilis
            Dvir\GJ22588
            Drosophila mojavensis
            Dmoj\GI23072
            Drosophila grimshawi
            Dgri\GH23629
            Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915004Z )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Musca domestica
            House fly
            Mdoa\17024516
            Glossina morsitans
            Tsetse fly
            Gmor\GMOY005630
            Lucilia cuprina
            Australian sheep blowfly
            Lcup\FF38_11648
            Mayetiola destructor
            Hessian fly
            Mdes\Mdes012159
            Aedes aegypti
            Yellow fever mosquito
            Aaeg\AAEL007589
            Anopheles darlingi
            American malaria mosquito
            Adar\ADAC006369
            Anopheles gambiae
            Malaria mosquito
            Agam\AGAP004320
            Culex quinquefasciatus
            Southern house mosquito
            Cqui\CPIJ002759
            Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01N6 )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Bombyx mori
            Silkmoth
            Bmor\BGIBMGA002322
            Bombyx mori
            Silkmoth
            Bmor\BGIBMGA000242
            Bombyx mori
            Silkmoth
            Bmor\BGIBMGA002321
            Bombyx mori
            Silkmoth
            Bmor\BGIBMGA000241
            Danaus plexippus
            Monarch butterfly
            Dple\DPOGS203087-PA
            Danaus plexippus
            Monarch butterfly
            Dple\DPOGS203258-PA
            Heliconius melpomene
            Postman butterfly
            Hmel\HMEL005706
            Heliconius melpomene
            Postman butterfly
            Hmel\HMEL002352
            Heliconius melpomene
            Postman butterfly
            Hmel\HMEL005298
            Apis florea
            Little honeybee
            Aflo\2211668628
            Apis florea
            Little honeybee
            Aflo\2147496643
            Apis mellifera
            Western honey bee
            Amel\GB50535
            Apis mellifera
            Western honey bee
            Amel\GB51337
            Bombus impatiens
            Common eastern bumble bee
            Bimp\BIMP19238
            Bombus impatiens
            Common eastern bumble bee
            Bimp\BIMP17702
            Bombus terrestris
            Buff-tailed bumblebee
            Bter\15210934
            Bombus terrestris
            Buff-tailed bumblebee
            Bter\15213308
            Linepithema humile
            Argentine ant
            Lhum\LH22049
            Linepithema humile
            Argentine ant
            Lhum\LH12202
            Megachile rotundata
            Alfalfa leafcutting bee
            Mrot\MROTU:002cc9
            Megachile rotundata
            Alfalfa leafcutting bee
            Mrot\MROTU:002dad
            Nasonia vitripennis
            Parasitic wasp
            Nvit\Nasvi2EG003329
            Nasonia vitripennis
            Parasitic wasp
            Nvit\Nasvi2EG016014
            Dendroctonus ponderosae
            Mountain pine beetle
            Dpod\YQE_08518
            Dendroctonus ponderosae
            Mountain pine beetle
            Dpod\YQE_08013
            Tribolium castaneum
            Red flour beetle
            Tcas\TC012454
            Tribolium castaneum
            Red flour beetle
            Tcas\TC034453
            Pediculus humanus
            Human body louse
            Phum\PHUM336900
            Pediculus humanus
            Human body louse
            Phum\PHUM252200
            Rhodnius prolixus
            Kissing bug
            Rpro\RPRC005582
            Rhodnius prolixus
            Kissing bug
            Rpro\RPRC012069
            Cimex lectularius
            Bed bug
            Clec\CLEC000680
            Cimex lectularius
            Bed bug
            Clec\CLEC011694
            Cimex lectularius
            Bed bug
            Clec\CLEC000681
            Acyrthosiphon pisum
            Pea aphid
            Apim\ACYPI001490
            Acyrthosiphon pisum
            Pea aphid
            Apim\ACYPI008946
            Zootermopsis nevadensis
            Nevada dampwood termite
            Znev\Znev_16516
            Zootermopsis nevadensis
            Nevada dampwood termite
            Znev\Znev_11441
            Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01L7 )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Strigamia maritima
            European centipede
            Smar\SMAR000811
            Strigamia maritima
            European centipede
            Smar\SMAR004951
            Ixodes scapularis
            Black-legged tick
            Isca\ISCW021297
            Ixodes scapularis
            Black-legged tick
            Isca\ISCW003358
            Stegodyphus mimosarum
            African social velvet spider
            Smim\KFM70972.1
            Stegodyphus mimosarum
            African social velvet spider
            Smim\KFM65182.1
            Stegodyphus mimosarum
            African social velvet spider
            Smim\KFM70970.1
            Stegodyphus mimosarum
            African social velvet spider
            Smim\KFM65177.1
            Tetranychus urticae
            Two-spotted spider mite
            Turt\tetur31g01970
            Tetranychus urticae
            Two-spotted spider mite
            Turt\tetur33g01610
            Tetranychus urticae
            Two-spotted spider mite
            Turt\tetur01g08450
            Tetranychus urticae
            Two-spotted spider mite
            Turt\tetur33g01670
            Daphnia pulex
            Water flea
            Dpux\DAPPUDRAFT_313508
            Daphnia pulex
            Water flea
            Dpux\DAPPUDRAFT_323931
            Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G007D )
            Organism
            Common Name
            Gene
            Multiple Dmel Genes in this Orthologous Group
            Strongylocentrotus purpuratus
            Purple sea urchin
            Spur\Sp-Dock180
            Strongylocentrotus purpuratus
            Purple sea urchin
            Spur\Sp-Dock180_1
            Ciona intestinalis
            Vase tunicate
            Cint\ENSCING00000007386
            Ciona intestinalis
            Vase tunicate
            Cint\ENSCING00000009657
            Gallus gallus
            Domestic chicken
            Ggal\DOCK5
            Gallus gallus
            Domestic chicken
            Ggal\DOCK1
            Gallus gallus
            Domestic chicken
            Ggal\ENSGALG00000025829
            Gallus gallus
            Domestic chicken
            Ggal\DOCK2
            Gallus gallus
            Domestic chicken
            Ggal\ENSGALG00000027359
            Human Disease Model Data
            FlyBase Human Disease Model Reports
              Alleles Reported to Model Human Disease (Disease Ontology)
              Download
              Models ( 0 )
              Allele
              Disease
              Evidence
              References
              Interactions ( 0 )
              Allele
              Disease
              Interaction
              References
              Comments ( 0 )
               
              Human Orthologs (via DIOPT v7.1)
              Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
              Homo sapiens (Human)
              Gene name
              Score
              OMIM
              OMIM Phenotype
              Complementation?
              Transgene?
              DOCK1; dedicator of cytokinesis 1
              12 of 15
              601403
                DOCK2; dedicator of cytokinesis 2
                12 of 15
                603122
                DOCK5; dedicator of cytokinesis 5
                11 of 15
                616904
                  DOCK3; dedicator of cytokinesis 3
                  5 of 15
                  603123
                    DOCK4; dedicator of cytokinesis 4
                    3 of 15
                    607679
                      Functional Complementation Data
                      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                      Interactions
                      Summary of Physical Interactions
                      esyN Network Diagram
                      Show neighbor-neighbor interactions:
                      Select Layout:
                      Legend:
                      Protein
                      RNA
                      Selected Interactor(s)
                      Interactions Browser
                      View in Interactions Browser
                      Please look at the Interaction Group reports for full details of the physical interactions
                      protein-protein
                      Interacting group
                      Assay
                      References
                      mbc - Ced-12
                      anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot, two hybrid, anti tag western blot
                      (Geisbrecht et al., 2008, Ishimaru et al., 2004)
                      mbc - Crk
                      anti tag coimmunoprecipitation, anti tag western blot, two hybrid
                      (Balagopalan et al., 2006, Ishimaru et al., 2004)
                      mbc - rols
                      anti tag coimmunoprecipitation, anti tag western blot
                      (Chen and Olson, 2001)
                      RNA-RNA
                      Interacting group
                      Assay
                      References
                      mbc - mir-9a
                      quantitative reverse transcription pcr
                      (Yatsenko and Shcherbata, 2014)
                      RNA-protein
                      Interacting group
                      Assay
                      References
                      mbc - DENR
                      anti bait coimmunoprecipitation, quantitative reverse transcription pcr
                      (Schleich et al., 2014)
                      Summary of Genetic Interactions
                      esyN Network Diagram
                      esyN Network Key:
                      Suppression
                      Enhancement
                      View in Interactions Browser
                      Please look at the allele data for full details of the genetic interactions
                      Starting gene(s)
                      Interaction type
                      Interacting gene(s)
                      Reference
                      mbc
                      enhanceable
                      Ced-12
                      (Geisbrecht et al., 2008)
                      mbc
                      enhanceable
                      Crk
                      (Geisbrecht et al., 2008)
                      mbc
                      suppressible
                      Rac1
                      (Haralalka et al., 2011)
                      Ced-12|mbc
                      suppressible
                      Rac1|Rac2
                      (Liu et al., 2013, Geisbrecht et al., 2008)
                      mbc
                      enhanceable
                      Sos
                      (Lu et al., 2014)
                      mbc
                      enhanceable
                      spg
                      (Biersmith et al., 2011)
                      Starting gene(s)
                      Interaction type
                      Interacting gene(s)
                      Reference
                      Ced-12
                      enhanceable
                      mbc
                      (Geisbrecht et al., 2008)
                      Crk
                      enhanceable
                      mbc
                      (Geisbrecht et al., 2008)
                      msk
                      suppressible
                      Ced-12|mbc
                      (Liu et al., 2013)
                      msk
                      enhanceable
                      mbc
                      (Liu et al., 2013)
                      msk
                      suppressible
                      mbc
                      (Liu et al., 2013)
                      Pvr
                      suppressible
                      mbc
                      (Duchek et al., 2001)
                      Pvr
                      enhanceable
                      mbc
                      (Ishimaru et al., 2004)
                      Rac1
                      suppressible
                      Ced-12|mbc
                      (Geisbrecht et al., 2008)
                      Rac1
                      suppressible
                      mbc
                      (Nolan et al., 1998)
                      Rac2
                      suppressible
                      mbc
                      (Nolan et al., 1998)
                      slbo
                      suppressible
                      mbc
                      (Duchek et al., 2001)
                      Sos
                      enhanceable
                      mbc
                      (Lu et al., 2014)
                      External Data
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      DroID - A comprehensive database of gene and protein interactions.
                      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                      Pathways
                      Gene Group - Pathway Membership (FlyBase)
                      Platelet-Derived Growth Factor-Vascular Endothelial Growth Factor Receptor-Related Signaling Pathway Core Components -
                      PDGF/VEGF-receptor related (Pvr) encodes a receptor tyrosine kinase activated by the binding of PDGF- and VEGF-related factors (Pvf1,Pvf2 or Pvf3). Pvr has been shown to activate the canonical Ras/Raf/MAP kinase (ERK) cascade, the PI3K kinase pathway, TORC1 (FBrf0222697), Rho family small GTPases (FBrf0221764, FBrf0180198) and the JNK cascade (FBrf0180198), in a context-dependant manner. (Adapted from FBrf0222697 and FBrf0221727).
                      External Data
                      Linkouts
                      Genomic Location and Detailed Mapping Data
                      Chromosome (arm)
                      3R
                      Recombination map
                      3-80
                      Cytogenetic map
                      95B5-95B7
                      Sequence location
                      3R:23,781,765..23,801,634 [+]
                      FlyBase Computed Cytological Location
                      Cytogenetic map
                      Evidence for location
                      95B5-95B7
                      Limits computationally determined from genome sequence between P{PZ}l(3)0468404684 and P{PZ}Atg600096
                      Experimentally Determined Cytological Location
                      Cytogenetic map
                      Notes
                      References
                      95B-95C
                      (Erickson, 1997.6.10)
                      Experimentally Determined Recombination Data
                      Location
                      3-80
                      (FlyBase, 2016)
                      3-82.1
                      (Comeron et al., 2012)
                      Left of (cM)
                      (Nolan et al., 1998)
                      Right of (cM)
                      (Nolan et al., 1998)
                      Notes
                      Stocks and Reagents
                      Stocks (23)
                      Genomic Clones (36)
                      cDNA Clones (53)
                       

                      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                      cDNA clones, fully sequences
                      BDGP DGC clones
                      Other clones
                      Drosophila Genomics Resource Center cDNA clones

                      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                      cDNA Clones, End Sequenced (ESTs)
                      BDGP DGC clones
                      Other clones
                      RNAi and Array Information
                      Linkouts
                      DRSC - Results frm RNAi screens
                      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                      Antibody Information
                      Laboratory Generated Antibodies
                       
                      polyclonal
                      (Erickson et al., 1997)
                      Commercially Available Antibodies
                       
                      Other Information
                      Relationship to Other Genes
                      Source for database identify of
                      Source for database merge of
                      Additional comments
                      Other Comments
                      mbc is both necessary and sufficient in the fusion competent myoblasts for fusion with founder cells during embryonic somatic muscle development.
                      (Haralalka et al., 2011)
                      mbc is needed in wild-type cells for the death of Minute neighbours in cell competition experiments.
                      (Li and Baker, 2007)
                      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
                      (Kiger et al., 2003)
                      dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
                      (Rogers et al., 2003)
                      mbc is required for the formation of syncytia within the visceral musculature of the midgut.
                      (Klapper et al., 2002)
                      The mbc product functions as a specific upstream regulator of Rac1 activity that mediates several morphogenetic processes in embryogenesis.
                      (Nolan et al., 1998)
                      Light and electron microscopy are used to study the process of myoblast fusion in mbc mutants.
                      (Doberstein et al., 1997)
                      Neuromuscular junctions (NMJ) can form on founder cells in the absence of myoblast fusion these NMJs have morphological and physiological properties closely resembling those of wild type. A smaller surface area of the fibre than wild type is devoted to the NMJ.
                      (Prokop et al., 1996)
                      mbc is required for the fusion of myoblasts into multinucleate muscles. The phenotype of mbc mutants provides evidence in support of the founder cell hypothesis of muscle development.
                      (Rushton et al., 1995)
                      Origin and Etymology
                      Discoverer
                      Etymology
                      Identification
                      External Crossreferences and Linkouts ( 55 )
                      Crossreferences
                      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                      Other crossreferences
                      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                      InterPro - A database of protein families, domains and functional sites
                      Linkouts
                      BioGRID - A database of protein and genetic interactions.
                      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
                      DroID - A comprehensive database of gene and protein interactions.
                      DRSC - Results frm RNAi screens
                      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                      Flygut - An atlas of the Drosophila adult midgut
                      FlyMine - An integrated database for Drosophila genomics
                      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                      KEGG Genes - Molecular building blocks of life in the genomic space.
                      modMine - A data warehouse for the modENCODE project
                      Synonyms and Secondary IDs (13)
                      Reported As
                      Symbol Synonym
                      CG10379
                      (Meadows, 2017.1.11, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Abmayr and Pavlath, 2012, Ni et al., 2010.12.1, Wittkopp et al., 2008, Rogers et al., 2003)
                      DOCK180
                      (Ridley, 2001)
                      Dm MBC
                      (Meller et al., 2005)
                      Dock180
                      (Biersmith et al., 2015, Meller et al., 2005)
                      MBC
                      (Freeman, 2015, Donaldson, 2003)
                      MBC/DOCK180
                      (Li and Baker, 2007)
                      Mbc
                      (Enomoto et al., 2018, Richardson and Portela, 2018, Nainu et al., 2017, Bothe and Baylies, 2016, Biersmith et al., 2015, Lu et al., 2014, Levayer and Moreno, 2013, Tran et al., 2013, Abmayr and Pavlath, 2012, Chen et al., 2007, Landry et al., 2005, Meller et al., 2005, Ishimaru et al., 2004, Kockel et al., 2001)
                      Su(rac)1
                      (Stronach and Perrimon, 1999, Nolan et al., 1998)
                      dock180
                      (McPhee et al., 2010)
                      mbc
                      (Al-Ramahi et al., 2018, Tsai et al., 2018, Winfree et al., 2017, Doiguchi et al., 2016, Gene Disruption Project members, 2016-, Aradska et al., 2015, Wang et al., 2015, Ashwal-Fluss et al., 2014, Haralalka et al., 2014, Luck et al., 2014, Rudolf et al., 2014, Schleich et al., 2014, Shlyueva et al., 2014, Tasdemir-Yilmaz and Freeman, 2014, Bonn et al., 2013, Kwon et al., 2013, Levayer and Moreno, 2013, Pirraglia et al., 2013, Sopko and Perrimon, 2013, Wilson et al., 2013, Zhu and Zhang, 2013, Bryantsev et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Kelsey et al., 2012, Ziegenfuss et al., 2012, Biersmith et al., 2011, Haralalka et al., 2011, Jin et al., 2011, Kazemian et al., 2011, Nagaosa et al., 2011, Ohsawa et al., 2011, Arancio et al., 2010, Bulchand et al., 2010, McPhee et al., 2010, Kuraishi et al., 2009, Liu et al., 2009, Shelton et al., 2009, Wolfstetter et al., 2009, Christensen et al., 2008.4.15, Geisbrecht et al., 2008, Wittkopp et al., 2008, Bakal et al., 2007, Beckett and Baylies, 2007, Buszczak et al., 2007, Estrada et al., 2007, Franklin-Dumont et al., 2007, Geisbrecht et al., 2007, Kesper et al., 2007, Kim et al., 2007, Li and Baker, 2007, Massarwa et al., 2007, Richardson et al., 2007, Zeitlinger et al., 2007, Balagopalan et al., 2006, Borghese et al., 2006, Estrada et al., 2006, Menon et al., 2005, Pütz et al., 2005, Chen et al., 2004, Paladi and Tepass, 2004)
                      Name Synonyms
                      Myoblast City
                      (Biersmith et al., 2015, Donaldson, 2003)
                      Myoblast city
                      (Wood and Martin, 2017, Amoyel and Bach, 2014, Reed et al., 2012, Bulchand et al., 2010, Geisbrecht et al., 2008, Chen et al., 2007, Geisbrecht et al., 2007, Cho et al., 2005, Pütz et al., 2005, Rogers et al., 2003)
                      myoblast city
                      (Lu et al., 2014, Levayer and Moreno, 2013, Pirraglia et al., 2013, Jin et al., 2011, McPhee et al., 2010, Wolfstetter et al., 2009, Balagopalan et al., 2006, Meller et al., 2005, Menon et al., 2005, Chen et al., 2004, Paladi and Tepass, 2004, Rushton et al., 1995)
                      Secondary FlyBase IDs
                        Datasets (0)
                        Study focus (0)
                        Experimental Role
                        Project
                        Project Type
                        Title
                        References (204)