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Gene Dmel\mbc

General Information
Symbol	Dmel\mbc	Species	D. melanogaster
Name	myoblast city	Annotation symbol	CG10379
Feature type	protein_coding_gene	FlyBase ID	FBgn0015513
Gene Model Status	Current	Stock availability	16 publicly available
Also Known As	Dock180, Su(rac)1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	95B5-95B7	Sequence location	3R:19,607,487..19,627,356 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene myoblast city is referred to in FlyBase by the symbol Dmel\mbc (CG10379, FBgn0015513). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; Rho guanyl-nucleotide exchange factor activity. There is experimental evidence that it is involved in the biological process: myoblast fusion; central nervous system development; dorsal closure; anterior Malpighian tubule development; border follicle cell migration; actin cytoskeleton organization; larval visceral muscle development; activation of Rac GTPase activity; cell competition in a multicellular organism; cytoskeleton organization. 64 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; organ system subdivision; non-connected developing system; anatomical structure; portion of tissue; embryonic/larval excretory system; ISN innervated muscle of A1-7; late extended germ band embryo; extended germ band embryo; hypodermal muscle of larval abdomen; embryonic hindgut; adult segment. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: C2 calcium-dependent membrane targeting; DHR-1 domain; DHR-2 domain; Dedicator of cytokinesis; Dedicator of cytokinesis A; Dedicator of cytokinesis C-terminal; Src homology-3 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult fat body, inseminated spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631013_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mbc. Gene sequence location is 3R:19607487..19627356.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02	Controlled Vocabulary Terms extrinsic to plasma membrane Rho guanyl-nucleotide exchange factor activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95B5-95B7   Limits computationally determined from genome sequence between P{PZ}l(3)0468404684 and P{PZ}Atg600096
Experimentally Determined Cytological Location
Cytogenetic map Notes References 95B-95C     (Erickson, 1997.6.10)
Experimentally Determined Recombination Data
Location
Left of (cM)	ca (Nolan et al., 1998)
Right of (cM)	e (Nolan et al., 1998)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mbc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.47 DGC clone SD06708 appears problematic: incomplete CDS Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mbc-RA FBtr0084459  NM_057796   7351   1970 mbc-RB FBtr0304729  NM_001260338   7465   2008
Additional Transcript Data & Comments
Reported size (kB)	7.5 (northern blot) (Erickson et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mbc-PA   FBpp0083850   226.5   1970   7.25     NP_477144   AAF56191 mbc-PB   FBpp0293271   230.7   2008   7.45     NP_001247267   AFH06585
Additional Polypeptide Data & Comments
Reported size (kDa)	1970 (aa); 226 (kD predicted) (Erickson et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Src homology-3 domain (IPR001452) C2 calcium-dependent membrane targeting (IPR000008) DHR-2 domain (IPR027357) Dedicator of cytokinesis A (IPR026799) Dedicator of cytokinesis (IPR026791) DHR-1 domain (IPR027007) Dedicator of cytokinesis C-terminal (IPR010703)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56191   AC008201   AF007805 AAB69648   AE014297 AFH06585   AI534176   AQ073771   AY122231 AAM52743   AY122254 AAM52766   BG641089   BI236909   BI368816   BI632794   BI635134   BI639891   BI641814   BP549612   BP549613   BT030856   BT100188   BZ748829   CO154663   CO186018   CO263519   CO269839   CO269854   CO276116   CO293792   CO301007   CO303432   CO306514   CO314098   CO314681   CO321867   CO330952   CO331837   CO333187   CZ468925   CZ469040   CZ469714   CZ469731   CZ469938   CZ488669   EC062517   EC062909   EC067743   EC071233   EC196901   EC205368   EC206177   EC208299   EC217598   EC226055   EC231836   EC232002   EC235823   EC239515   EC248754   EC251894   EC260994   EV579789   EV595074   EV599766   FE863340   GH739205   GH739245   GH740981   GH744989   GH746025   GH764890   GH765951   GH766287   GH767086   GH940833
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VCH4 Q8MQY7 Q8MQW7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   pole plasm   (Fisher et al., 2012) Comment:maternally deposited organism   • pole plasm (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) dorsal pharyngeal muscle primordium   (Fisher et al., 2012) longitudinal visceral muscle primordium   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium visceral muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium trunk mesoderm   (Fisher et al., 2012) embryonic stage 12   ectoderm | striped   (Erickson et al., 1997) embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) longitudinal visceral muscle fiber   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Erickson et al., 1997) embryonic stage 1 -- 9     (Erickson et al., 1997) Comment:reference states 0-4 hr AEL embryonic stage 4   pole cell   (Erickson et al., 1997) embryonic stage 5   organism | lateral   (Erickson et al., 1997) embryonic stage 7 -- 12   ectoderm   (Erickson et al., 1997) embryonic stage 12   endoderm   (Erickson et al., 1997) embryonic stage 12 -- 14   somatic mesoderm   (Erickson et al., 1997) embryonic stage 12 -- 17   visceral mesoderm   (Erickson et al., 1997) embryonic stage 14   embryonic/larval visceral muscle   (Erickson et al., 1997) embryonic stage 16   embryonic/larval dorsal vessel   (Erickson et al., 1997) pupal stage     (Erickson et al., 1997) adult stage | female     (Erickson et al., 1997) adult stage | male     (Erickson et al., 1997)
Additional Descriptive Data
	Both the cardial and pericardial cells of the dorsal vessel express mbc transcript. No mbc transcript is detected in mature muscles. (Erickson et al., 1997)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   pole cell   (Erickson et al., 1997) embryonic stage 12   endoderm   (Erickson et al., 1997) myoblast   (Erickson et al., 1997) ectoderm   (Erickson et al., 1997) embryonic stage 14   embryonic/larval visceral muscle   (Erickson et al., 1997) embryonic stage 16   embryonic/larval dorsal vessel   (Erickson et al., 1997)
Additional Descriptive Data
	mbc protein expression correlates approximately with mbc transcript expression. Although mbc transcript is not detected in mature muscles, mbc protein is detected at low levels in mature muscles. Colocalization studies with Mef2 protein indicate that mbc protein is present in ectodermal and endodermal germ layers, with ectodermal expression being restricted to the epidermal layer, and that mbc is expressed in myoblasts. (Erickson et al., 1997)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Erickson et al., 1997) extrinsic to plasma membrane inferred from direct assay (Haralalka et al., 2011)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult fat body, inseminated spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body  (542.4) Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca  (640.5) Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body  542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca  640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    213.9   NA  Eye    99.275   NA  Brain    210.7   207.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    277.7   NA  Crop    215.3   215.1  Midgut    312.1   210  Hindgut    202.4   122.3  Malpighian Tubules    260.8   202.3  Fat Body    542.4   164.7  Salivary Gland    171.5   NA  Heart    243.225   183.925  Trachea    NA   NA  VirginFemale Spermatheca    498.3   NA  InseminatedFemale Spermatheca    640.5   NA  Ovary    428.1   NA  Testis    51.5   NA  Male Accessory Gland    92.9   105.15  Carcass    249.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female    35 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female  (35) ovary, mated 4-day female  (30) testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female    35 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female    35 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female    35 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female  (35) ovary, mated 4-day female  30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female    35 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    11 central nervous system, pupae P8    14 head, virgin 1-day female    14 head, virgin 4-day female    11 head, virgin 20-day female    18 head, mated 1-day female    11 head, mated 4-day female    15 head, mated 20-day female    15 head, mated 1-day male    15 head, mated 4-day male    17 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    5 digestive system, larvae L3 wandering    14 digestive system, 1-day adult    9 digestive system, 4-day adult    10 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    14 fat body, pupae P8    11 carcass, larvae L3 wandering    10 carcass, 1-day adult    10 carcass, 4-day adult    14 carcass, 20-day adult    13 ovary, virgin 4-day female    35 ovary, mated 4-day female    30 testis, mated 4-day male    2 accessory gland, mated 4-day male    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr    56 embryo 06-08hr    53 embryo 08-10hr    52 embryo 10-12hr    33 embryo 12-14hr    36 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr  (56) embryo 06-08hr  (53) embryo 08-10hr  (52) embryo 10-12hr  (33) embryo 12-14hr  (36) embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day  26 adult female 30day  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr    56 embryo 06-08hr    53 embryo 08-10hr    52 embryo 10-12hr    33 embryo 12-14hr    36 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr    56 embryo 06-08hr    53 embryo 08-10hr    52 embryo 10-12hr    33 embryo 12-14hr    36 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day    26 adult female 30day    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr    56 embryo 06-08hr    53 embryo 08-10hr    52 embryo 10-12hr    33 embryo 12-14hr    36 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr  (56) embryo 06-08hr  (53) embryo 08-10hr  (52) embryo 10-12hr  (33) embryo 12-14hr  (36) embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day  26 adult female 30day  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr    56 embryo 06-08hr    53 embryo 08-10hr    52 embryo 10-12hr    33 embryo 12-14hr    36 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    25 embryo 02-04hr    25 embryo 04-06hr    56 embryo 06-08hr    53 embryo 08-10hr    52 embryo 10-12hr    33 embryo 12-14hr    36 embryo 14-16hr    19 embryo 16-18hr    23 embryo 18-20hr    19 embryo 20-22hr    11 embryo 22-24hr    14 larva L1    10 larva L2    8 larva L3 12hr old    8 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    15 white prepupae new    23 white prepupae 12hr    22 white prepupae 24hr    19 pupae 2d postWPP    14 pupae 3d postWPP    13 pupae 4d postWPP    11 adult male 01day    13 adult male 05day    13 adult male 30day    13 adult female 01day    18 adult female 05day    26 adult female 30day    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    99 embryonic GM2    60 embryonic Kc167    26 embryonic S1    23 embryonic S3    89 leg disc CME L1    68 wing disc CME-W2    86 wing disc ML-DmD8    78 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    72 wing disc ML-DmD21    12 wing disc ML-DmD32    104 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    39 antennal disc ML-DmD20-c5    43 mixed discs ML-DmD4-c1    58 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    52 tumorous blood cells mbn2    36 ovary fGS/OSS    36 ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (34) Schneider line 2 Sg4  (43) embryonic 1182-4H  (99) embryonic GM2  (60) embryonic Kc167  26 embryonic S1    23 embryonic S3  (89) leg disc CME L1  (68) wing disc CME-W2  (86) wing disc ML-DmD8  (78) wing disc ML-DmD9  (87) wing disc ML-DmD16-c3  (72) wing disc ML-DmD21    12 wing disc ML-DmD32  (104) haltere disc ML-DmD17-c3  (63) eye-antennal disc ML-DmD11  (39) antennal disc ML-DmD20-c5  (43) mixed discs ML-DmD4-c1  (58) CNS ML-DmBG1-c1  (31) CNS ML-DmBG2-c2  (52) tumorous blood cells mbn2  (36) ovary fGS/OSS  (36) ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    99 embryonic GM2    60 embryonic Kc167    26 embryonic S1    23 embryonic S3    89 leg disc CME L1    68 wing disc CME-W2    86 wing disc ML-DmD8    78 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    72 wing disc ML-DmD21    12 wing disc ML-DmD32  104 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    39 antennal disc ML-DmD20-c5    43 mixed discs ML-DmD4-c1    58 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    52 tumorous blood cells mbn2    36 ovary fGS/OSS    36 ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    99 embryonic GM2    60 embryonic Kc167    26 embryonic S1    23 embryonic S3    89 leg disc CME L1    68 wing disc CME-W2    86 wing disc ML-DmD8    78 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    72 wing disc ML-DmD21    12 wing disc ML-DmD32    104 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    39 antennal disc ML-DmD20-c5    43 mixed discs ML-DmD4-c1    58 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    52 tumorous blood cells mbn2    36 ovary fGS/OSS    36 ovary OSC    21 ovary OSS    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    99 embryonic GM2    60 embryonic Kc167    26 embryonic S1    23 embryonic S3    89 leg disc CME L1    68 wing disc CME-W2    86 wing disc ML-DmD8    78 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    72 wing disc ML-DmD21    12 wing disc ML-DmD32    104 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    39 antennal disc ML-DmD20-c5    43 mixed discs ML-DmD4-c1    58 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    52 tumorous blood cells mbn2    36 ovary fGS/OSS    36 ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (34) Schneider line 2 Sg4  (43) embryonic 1182-4H  (99) embryonic GM2  (60) embryonic Kc167  26 embryonic S1    23 embryonic S3  (89) leg disc CME L1  (68) wing disc CME-W2  (86) wing disc ML-DmD8  (78) wing disc ML-DmD9  (87) wing disc ML-DmD16-c3  (72) wing disc ML-DmD21    12 wing disc ML-DmD32  (104) haltere disc ML-DmD17-c3  (63) eye-antennal disc ML-DmD11  (39) antennal disc ML-DmD20-c5  (43) mixed discs ML-DmD4-c1  (58) CNS ML-DmBG1-c1  31 CNS ML-DmBG2-c2  (52) tumorous blood cells mbn2  (36) ovary fGS/OSS  (36) ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    99 embryonic GM2    60 embryonic Kc167    26 embryonic S1    23 embryonic S3    89 leg disc CME L1    68 wing disc CME-W2    86 wing disc ML-DmD8    78 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    72 wing disc ML-DmD21    12 wing disc ML-DmD32  104 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    39 antennal disc ML-DmD20-c5    43 mixed discs ML-DmD4-c1    58 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    52 tumorous blood cells mbn2    36 ovary fGS/OSS    36 ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    34 Schneider line 2 Sg4    43 embryonic 1182-4H    99 embryonic GM2    60 embryonic Kc167    26 embryonic S1    23 embryonic S3    89 leg disc CME L1    68 wing disc CME-W2    86 wing disc ML-DmD8    78 wing disc ML-DmD9    87 wing disc ML-DmD16-c3    72 wing disc ML-DmD21    12 wing disc ML-DmD32    104 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    39 antennal disc ML-DmD20-c5    43 mixed discs ML-DmD4-c1    58 CNS ML-DmBG1-c1    31 CNS ML-DmBG2-c2    52 tumorous blood cells mbn2    36 ovary fGS/OSS    36 ovary OSC    21 ovary OSS    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    10 cold shock, 4-day adult    8 heat shock, 4-day adult    12 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    11 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    16 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    11 Ethanol 2.5% 3 hrs, larvae L3    25 Ethanol 5% 3 hrs, larvae L3    7 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    9 Paraquat 5 mM 48 hrs, 4-day adult    21 Paraquat 10 mM 48 hrs, 4-day adult    23 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	BDGP expression data • SD06708 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015513 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10379-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for mbc Stages(s) 1-3 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal mbcCB04603 lethal | embryonic stage | recessive mbcC1 mbcC2 mbcC3 mbcS4 lethal | recessive mbc1.63 mbc2.35 mbc3.5 mbc4.25 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 mbcGD6965 Other Phenotypes Allele neuroanatomy defective | embryonic stage mbcD11.2 visible, with Scer\GAL4pnr-MD237 mbcGD6965 Phenotype manifest in Allele A1-7 dorsal acute muscle 1 mbcTT261 A1-7 dorsal acute muscle 1 | embryonic stage 15 mbcD11.2 axon & ventral nerve cord mbc1.63 mbc4.25 border follicle cell, with Scer\GAL4slbo.2.6 mbcdsRNA.Scer\UAS border follicle cell | somatic clone mbcC2 mbcD11.2 chaeta | supernumerary, with Scer\GAL4pnr-MD237 mbcGD6965 circular visceral muscle fiber mbcC1 circular visceral muscle primordium mbcC1 commissure mbc1.63 mbc4.25 embryonic/larval anterior Malpighian tubule mbcC1 embryonic/larval Malpighian tubule mbcC1 embryonic/larval muscle system mbcC1 embryonic/larval somatic muscle mbcC1 mbcC2 mbcC3 mbcF12.7 mbcS4 mbcunspecified embryonic dorsal epidermis mbc1.63 mbc2.35 mbc3.5 mbc4.25 mbcC1 mbcF12.7 embryonic Malpighian tubule mbcunspecified embryonic myoblast mbc1.63 mbc2.35 mbc3.5 mbc4.25 mbcC1 mbcC2 mbcC3 mbcD11.2 mbcS4 embryonic myoblast | germline clone mbcD11.2 embryonic visceral muscle mbcC1 excretory star cell mbcC1 fusion competent cell | embryonic stage mbcC1 mbcC2 mbcC3 mbcS4 lateral longitudinal fascicle mbcD11.2 longitudinal connective mbc1.63 mbc4.25 longitudinal muscle mbcC1 mesothoracic tergum, with Scer\GAL4pnr-MD237 mbcGD6965 midgut constriction mbcC1 mbcF12.7 muscle founder cell | embryonic stage mbcD11.2 myoblast mbcC1 presumptive embryonic/larval muscle system mbcD11.2 presumptive embryonic/larval muscle system | germline clone mbcD11.2 thorax, with Scer\GAL4pnr-MD237 mbcdsRNA.Scer\UAS
Classical Alleles ( 42 )
For All Classical Alleles Show
Allele of mbc Class Mutagen Stocks Known lesion mbcC1 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 1 -- mbcC2 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 1 -- mbcC3 amorphic allele - genetic evidence ethyl methanesulfonate 1 -- mbcc04167 piggyBac transposase 1 -- mbcc05056 piggyBac transposase 1 -- mbcc05087 piggyBac transposase 1 -- mbcc05391 piggyBac transposase 1 -- mbcCB-0487-3 1 -- mbcD11.2 loss of function allele ethyl methanesulfonate 1 Yes mbcEY01437 P-element activity 1 -- mbcf06027 piggyBac transposase 1 -- mbcMI06051 1 -- mbcNP5474 1 -- mbcS4 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- mbc1.63 ethyl methanesulfonate 0 -- mbc2.35 ethyl methanesulfonate 0 -- mbc3.5 ethyl methanesulfonate 0 -- mbc4.25 ethyl methanesulfonate 0 -- mbcAC177 ethyl methanesulfonate 0 Yes mbcc03965 piggyBac transposase 0 -- mbcC14.3 ethyl methanesulfonate 0 Yes mbcC6.1 ethyl methanesulfonate 0 Yes mbcC9.2 ethyl methanesulfonate 0 Yes mbcCB04603 P-element activity 0 Yes mbcCPTI000332 piggyBac transposase 0 Yes mbcCPTI001082 piggyBac transposase 0 Yes mbcE26.2 ethyl methanesulfonate 0 Yes mbcE33.1 ethyl methanesulfonate 0 Yes mbcEP3135 P-element activity 0 -- mbcEPg36390 P-element activity 0 Yes mbcF12.7 ethyl methanesulfonate 0 Yes mbcF4.4 ethyl methanesulfonate 0 Yes mbcF6.4 loss of function allele ethyl methanesulfonate 0 Yes mbcF6.7 ethyl methanesulfonate 0 Yes mbcI6.6 ethyl methanesulfonate 0 Yes mbcJ342 ethyl methanesulfonate 0 Yes mbcO146 ethyl methanesulfonate 0 Yes mbcTT261 ethyl methanesulfonate 0 Yes mbcunspecified 0 -- mbcZZ1 ethyl methanesulfonate 0 Yes mbcZZ260 ethyl methanesulfonate 0 Yes mbczz351 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mbc Class Mutagen Stocks Known lesion mbcGD6965 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbcHMS00346 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbcHMS00604 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbcAct5C.T:SV5\V5,T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mbcCBS.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes mbcDockerF6.4.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes mbcdsRNA.cIa in vitro construct - RNAi 0 Yes mbcdsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mbcMtnA.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mbcNIG.10379R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mbcNPxxP.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes mbcNPxxP.Δ1807.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - deletion 0 Yes mbcNPxxP.ΔDHR1.Δ1807.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - deletion in vitro construct - amino acid replacement 0 Yes mbcScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes mbcScer\UAS.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mbcScer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion 0 Yes mbcSH3W47K.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes mbcΔ1807.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes mbcΔDHR1.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes mbcΔPRM.MtnA.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes mbcΔPRM.Scer\UAS.T:Ivir\HA1 in vitro construct - coding region fusion in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes mbcΔSH3.MtnA.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)BSC489 (Christensen et al., 2008.4.15) Df(3R)Exel9014 (Ryder, 2004.4.26) Df(3R)mbc-15A (Erickson et al., 1997) Df(3R)mbc-30 (Keller et al., 1998, Rushton et al., 1995, Erickson et al., 1997) Df(3R)mbc9 (Balagopalan et al., 2006) Df(3R)mbc-BG1 (Presgraves et al., 2003) Df(3R)mbc-F5.3 (Erickson et al., 1997) Df(3R)mbc-R1 (Rushton et al., 1995, Drysdale, 1996.6.25) Df(3R)mbc-R.1 (Erickson et al., 1997) Df(3R)nau-9 (Keller et al., 1998) Df(3R)γ20 (Keller et al., 1998)
Not disrupted in	Df(3R)CA (Keller et al., 1998, Presgraves et al., 2003) Df(3R)CA2 (Erickson et al., 1997) Df(3R)CA15 (Erickson et al., 1997) Df(3R)nau-4A (Keller et al., 1998) Df(3R)nau-11a4 (Keller et al., 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6965} NA P{NIG.10379R} NA P{TRiP.HMS00346} NA P{TRiP.HMS00604} NA P{UAS-mbc.B} NA P{UAS-mbc.CBS} NA P{UAS-mbc.DockerF6.4.HA} NA P{UAS-mbc.HA} NA P{UAS-mbc.IR} NA P{UAS-mbc.MYC} NA P{UAS-mbc.NPxxP.HA} NA P{UAS-mbc.NPxxP.Δ1807.HA} NA P{UAS-mbc.NPxxP.ΔDHR1.Δ1807.HA} NA P{UAS-mbc.SH3W47K.HA} NA P{UAS-mbc.Δ1807.HA} NA P{UAS-mbc.ΔDHR1.HA} NA P{UAS-mbc.ΔPRM.HA} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}mbcEP3135 NA P{EPg}mbcEPg36390 NA P{EPgy2}mbcEY01437 NA miscellaneous insertions Mi{MIC}mbcMI06051 NA P{PTT-GB}mbcCB04603 NA P{RS3}mbcCB-0487-3 NA PBac{602.P.SVS-1}mbcCPTI000332 NA PBac{602.P.SVS-1}mbcCPTI001082 NA PBac{PB}mbcc03965 NA PBac{PB}mbcc04167 NA PBac{PB}mbcc05056 NA PBac{PB}mbcc05087 NA PBac{PB}mbcc05391 NA PBac{WH}mbcf06027 NA insertion of enhancer trap binary system P{GawB}mbcNP5474 No
Gene Ontology: Function, Process & Cellular Component ( 17 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with rols (Chen and Olson, 2001) Rho guanyl-nucleotide exchange factor activity inferred from genetic interaction with Rac1 (Haralalka et al., 2011)
Biological Process
CV term Evidence References
actin cytoskeleton organization inferred from mutant phenotype (Richardson et al., 2007, Haralalka et al., 2011) activation of Rac GTPase activity inferred from genetic interaction with Rac1 (Haralalka et al., 2011) inferred from genetic interaction with Rac1, Ced-12 (Geisbrecht et al., 2008) inferred from genetic interaction with Rac1, Ced-12, Rac2 (Geisbrecht et al., 2008) anterior Malpighian tubule development inferred from mutant phenotype (Puetz et al., 2005) border follicle cell migration inferred from mutant phenotype (Geisbrecht et al., 2008) cell competition in a multicellular organism inferred from mutant phenotype (Li and Baker, 2007) central nervous system development inferred from mutant phenotype (Biersmith et al., 2011) cytoskeleton organization inferred from mutant phenotype (Erickson et al., 1997) dorsal closure inferred from mutant phenotype (Erickson et al., 1997) larval visceral muscle development inferred from mutant phenotype (Klapper et al., 2002) myoblast fusion inferred from mutant phenotype (Rushton et al., 1995, Menon et al., 2005, Estrada et al., 2006, Richardson et al., 2007, Balagopalan et al., 2006, Shelton et al., 2009, Haralalka et al., 2011)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Erickson et al., 1997) extrinsic to plasma membrane inferred from direct assay (Haralalka et al., 2011)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cell morphogenesis traceable author statement (Noselli and Agnes, 1999) dorsal closure traceable author statement (Noselli and Agnes, 1999, Van Aelst and Symons, 2002, Harden, 2002) myoblast fusion traceable author statement (Dworak and Sink, 2002) skeletal muscle tissue development traceable author statement (Cooper, 2001)
Cellular Component
CV term Evidence References
ruffle traceable author statement (Noselli and Agnes, 1999)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mbc allele Gene References mbc1.63 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbc2.35 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbc3.5 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbc4.25 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbcD11.2 Pvr (Duchek et al., 2001, Ishimaru et al., 2004) mbcEPg36390 slbo (Duchek et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67754 DroID - A comprehensive database of gene and protein interactions. • FBgn0015513 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42817
Orthologs
OrthoDB Orthologs (82) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6HMJVK Dipteran inclusive ortholog search EOG6W9JBV Insect inclusive ortholog search EOG64J1CT Arthropod inclusive ortholog search EOG6573NC Metazoa inclusive ortholog search EOG6V4G18
Orthologs in Drosophila Species (EOG6HMJVK)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\mbc     Drosophila simulans   Dsim\GD21040     Drosophila sechellia   Dsec\GM26532 Y   Drosophila erecta   Dere\GG11230 Y   Drosophila yakuba   Dyak\GE10396 Y   Drosophila ananassae   Dana\GF16263 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10288 Y   Drosophila persimilis   Dper\GL23263 Y   Drosophila willistoni   Dwil\GK11109 Y   Drosophila virilis   Dvir\GJ22588 Y   Drosophila mojavensis   Dmoj\GI23072 Y   Drosophila grimshawi   Dgri\GH23629 Y
Orthologs in non-Drosophila Dipterans (EOG6W9JBV)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007589   Anopheles gambiae Malaria mosquito  Agam\AGAP004320   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002759
Orthologs in non-Dipteran Insects (EOG64J1CT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB50535 Y Apis mellifera Western honey bee  Amel\GB51337 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG016014 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003329 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12789 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13405 Y Atta cephalotes Leafcutter ant  Acep\ACEP22075 Y Atta cephalotes Leafcutter ant  Acep\ACEP24788 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO14312 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO16466 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15434 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL17964 Y Linepithema humile Argentine ant  Lhum\LH22049 Y Linepithema humile Argentine ant  Lhum\LH12202 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB16335 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB13200 Y Solenopsis invicta Red fire ant  Sinv\SINV18192 Y Solenopsis invicta Red fire ant  Sinv\SINV24844 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008946 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001490 Y Bombyx mori Silkmoth  Bmor\BGIBMGA002321 Y Bombyx mori Silkmoth  Bmor\BGIBMGA002322 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000241 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000242 Y Pediculus humanus Human body louse  Phum\PHUM336900 Y Pediculus humanus Human body louse  Phum\PHUM252200 Y Tribolium castaneum Red flour beetle  Tcas\TC012780 Y Tribolium castaneum Red flour beetle  Tcas\TC012454 Y
Orthologs in non-Insect Arthropods (EOG6573NC)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_323931 Y Daphnia pulex Water flea  Dpux\JGI_V11_313508 Y Ixodes scapularis Deer tick  Isca\ISCW021297 Y Ixodes scapularis Deer tick  Isca\ISCW003358 Y
Orthologs in non-Arthropod Metazoa (EOG6V4G18)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ced-5 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022636gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012009gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017644gn Y Danio rerio Zebrafish  Drer\dock4b Y Danio rerio Zebrafish  Drer\ENSDARG00000076213 Y Danio rerio Zebrafish  Drer\dock5 Y Danio rerio Zebrafish  Drer\dock1 Y Danio rerio Zebrafish  Drer\ENSDARG00000076562 Y Danio rerio Zebrafish  Drer\dock3 Y Xenopus tropicalis Western clawed frog  Xtro\dock1 Y Xenopus tropicalis Western clawed frog  Xtro\dock3 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031383 Y Xenopus tropicalis Western clawed frog  Xtro\dock2 Y Xenopus tropicalis Western clawed frog  Xtro\dock4 Y Gallus gallus Domestic chicken  Ggal\DOCK5 Y Gallus gallus Domestic chicken  Ggal\DOCK1 Y Gallus gallus Domestic chicken  Ggal\DOCK3 Y Gallus gallus Domestic chicken  Ggal\DOCK4 Y Gallus gallus Domestic chicken  Ggal\DOCK2 Y Mus musculus House mouse  Mmus\Dock5 Y Mus musculus House mouse  Mmus\Dock2 Y Mus musculus House mouse  Mmus\Dock4 Y Mus musculus House mouse  Mmus\Dock3 Y Mus musculus House mouse  Mmus\Dock1 Y Rattus norvegicus Norway rat  Rnor\Dock5_predicted Y Rattus norvegicus Norway rat  Rnor\Dock3_predicted Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000006932 Y Rattus norvegicus Norway rat  Rnor\Dock1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000004823 Y Homo sapiens Human  Hsap\DOCK5 Y Homo sapiens Human  Hsap\ENSG00000088538 Y Homo sapiens Human  Hsap\DOCK1 Y Homo sapiens Human  Hsap\ENSG00000128512 Y Homo sapiens Human  Hsap\DOCK2 Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\DOCK5     Hsap\ENSG00000088538     Hsap\DOCK1     Hsap\ENSG00000128512     Hsap\DOCK2
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM26532 Drosophila erecta Dere\GG11230 Drosophila yakuba Dyak\GE10396 Drosophila ananassae Dana\GF16263 Drosophila pseudoobscura pseudoobscura Dpse\GA10288 Drosophila persimilis Dper\GL23263 Drosophila willistoni Dwil\GK11109 Drosophila virilis Dvir\GJ22588 Drosophila mojavensis Dmoj\GI23072 Drosophila grimshawi Dgri\GH23629
Stocks & Reagents
Stocks Listed in FlyBase ( 16 )
Bloomington	43040 y1 w*; Mi{MIC}mbcMI06051 32355 y1 sc* v1; P{TRiP.HMS00346}attP2 33722 y1 sc* v1; P{TRiP.HMS00604}attP2 4952 st1 e1 mbcD11.2/TM3, Ser1
Harvard	- PBac{PB}mbcc04167 - PBac{PB}mbcc05056 - PBac{PB}mbcc05087 - PBac{PB}mbcc05391 - PBac{WH}mbcf06027
Kyoto	123198 w1118; P{RS3}mbcCB-0487-3 113750 w*; P{GawB}mbcNP5474 / TM6, P{UAS-lacZ.UW23-1}UW23-1
VDRC	v16044 w1118; P{GD6965}v16044
	More stocks available...
Genomic Clones ( 1 )
	BACR11E07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 57 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE54856 SD06708
Other clones	IP18282 MIP14651
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE33039 SD12332 SD16380 SD22594 SD25447 SD26666
Other clones	54667 60264 118205 121308 193668 214219.15 274333 281364 523988 CK01.13D.D6 CK01.15A.F7 CK01.106B.F7 CK01.125B.G6 CK01.129D.G1 CK01.145D.F9 Dmel_dig_012417_0691_1861 Dmel_neb_006474_0880_0389 Dmel_neb_010767_0707_0398 EC235823 EK015224 EK088724 EK096045 EK096125 EK119723 EK179864 EK186561 EK204837 EK235108 EK237427 EK241439 EK251304 EK296637 EK301816 EK306140 EN04546 FGM102D11 RM07042 RP001011381 RP001012227 RP001017550 RP001018205 RP001019162 RP001031023 RP001042649 RP001043012 RP001054177 RP003651652
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015513 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42817
Antibody Information
	polyclonal (Erickson et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	mbc is both necessary and sufficient in the fusion competent myoblasts for fusion with founder cells during embryonic somatic muscle development. (Haralalka et al., 2011) mbc is needed in wild-type cells for the death of Minute neighbours in cell competition experiments. (Li and Baker, 2007) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) mbc is required for the formation of syncytia within the visceral musculature of the midgut. (Klapper et al., 2002) The mbc product functions as a specific upstream regulator of Rac1 activity that mediates several morphogenetic processes in embryogenesis. (Nolan et al., 1998) Light and electron microscopy are used to study the process of myoblast fusion in mbc mutants. (Doberstein et al., 1997) Neuromuscular junctions (NMJ) can form on founder cells in the absence of myoblast fusion these NMJs have morphological and physiological properties closely resembling those of wild type. A smaller surface area of the fibre than wild type is devoted to the NMJ. (Prokop et al., 1996) mbc is required for the fusion of myoblasts into multinucleate muscles. The phenotype of mbc mutants provides evidence in support of the founder cell hypothesis of muscle development. (Rushton et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057796 NM_001260338 RefSeq (Proteins) • NP_477144 NP_001247267 DDBJ / EMBL / GenBank • AAB69648 AAF56191 AAM52743 AAM52766 AC008201 AF007805 AFH06585 AI534176 AQ073771 AY122231 AY122254 BI368816 BZ748829 CZ468925 CZ469040 CZ469714 CZ469731 CZ469938 CZ488669 Entrez Gene - A searchable database of RefSeq genes. • 42817 UniProtKB/TrEMBL • Q8MQW7 Q8MQY7 Q9VCH4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Src homology-3 domain (IPR001452) C2 calcium-dependent membrane targeting (IPR000008) DHR-2 domain (IPR027357) Dedicator of cytokinesis A (IPR026799) Dedicator of cytokinesis (IPR026791) DHR-1 domain (IPR027007) Dedicator of cytokinesis C-terminal (IPR010703) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • SD06708
Linkouts
	BioGRID - A database of protein and genetic interactions • 67754 DroID - A comprehensive database of gene and protein interactions. • FBgn0015513 DRSC - Results from RNAi screens. • FBgn0015513 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015513 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10379-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015513 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42817 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/myblcy1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42817 modMine - Data generated by the modENCODE project. • FBgn0015513
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG10379 (Rogers et al., 2003, Wittkopp et al., 2008, Ni et al., 2010.12.1) Dm MBC (Meller et al., 2005) Dock180 (Meller et al., 2005) DOCK180 (Ridley, 2001) dock180 (McPhee et al., 2010) Mbc (Landry et al., 2005, Ishimaru et al., 2004, Kockel et al., 2001, Chen et al., 2007, Meller et al., 2005) MBC (Donaldson, 2003) mbc (Geisbrecht et al., 2007, Menon et al., 2005, Estrada et al., 2006, Kazemian et al., 2011, Buszczak et al., 2007, Li and Baker, 2007, Arancio et al., 2010, Christensen et al., 2008.4.15, Puetz et al., 2005, Bakal et al., 2007, Ohsawa et al., 2011, Balagopalan et al., 2006, Kesper et al., 2007, Biersmith et al., 2011, Franklin-Dumont et al., 2007, Haralalka et al., 2011, Borghese et al., 2006, Kim et al., 2007, Massarwa et al., 2007, Richardson et al., 2007, Estrada et al., 2007, Beckett and Baylies, 2007, Geisbrecht et al., 2008, Chen et al., 2004, Paladi and Tepass, 2004, Liu et al., 2009, Shelton et al., 2009, Wolfstetter et al., 2009, Zeitlinger et al., 2007, Wittkopp et al., 2008, Kuraishi et al., 2009, Bulchand et al., 2010, McPhee et al., 2010, Bryantsev et al., 2012, Jin et al., 2011, Japanese National Institute of Genetics, 2012.5.21, Kelsey et al., 2012, Ziegenfuss et al., 2012) MBC/DOCK180 (Li and Baker, 2007) Su(rac)1 (Stronach and Perrimon, 1999, Nolan et al., 1998)
Name Synonym	Myoblast City (Donaldson, 2003) myoblast city (Rushton et al., 1995, Menon et al., 2005, Meller et al., 2005, Balagopalan et al., 2006, Chen et al., 2004, Paladi and Tepass, 2004, Wolfstetter et al., 2009, McPhee et al., 2010, Jin et al., 2011) Myoblast city (Geisbrecht et al., 2007, Cho et al., 2005, Rogers et al., 2003, Chen et al., 2007, Puetz et al., 2005, Geisbrecht et al., 2008, Reed et al., 2012, Bulchand et al., 2010)
Secondary FlyBase IDs
References ( 149 )
Generate a list of	All references relating to this gene ( 149 )
List References by type	Research paper  ( 69 ) Supplementary material  ( 9 ) Review  ( 34 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 18 ) FlyBase analysis  ( 2 ) Stock list  ( 1 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 9 )
	Bryantsev et al., 2012, Dev. Biol. 361(2): 191--207 Differential requirements for Myocyte Enhancer Factor-2 during adult myogenesis in Drosophila. [FBrf0217023] Kelsey et al., 2012, J. Cell Sci. 125(6): 1393--1400 Schnurri regulates hemocyte function to promote tissue recovery after DNA damage. [FBrf0218143] Reed et al., 2012, Cell. Microbiol. 14(4): 529--545 Rickettsia parkeri invasion of diverse host cells involves an Arp2/3 complex, WAVE complex and Rho-family GTPase-dependent pathway. [FBrf0217748] Ziegenfuss et al., 2012, Nat. Neurosci. 15(7): 979--987 Distinct molecular pathways mediate glial activation and engulfment of axonal debris after axotomy. [FBrf0218752] Biersmith et al., 2011, PLoS ONE 6(1): e16120 The DOCK Protein Sponge Binds to ELMO and Functions in Drosophila Embryonic CNS Development. [FBrf0212890] Haralalka et al., 2011, Development 138(8): 1551--1562 Asymmetric Mbc, active Rac1 and F-actin foci in the fusion-competent myoblasts during myoblast fusion in Drosophila. [FBrf0213340] Jin et al., 2011, Dev. Cell 20(5): 623--638 Competition between Blown Fuse and WASP for WIP Binding Regulates the Dynamics of WASP-Dependent Actin Polymerization In Vivo. [FBrf0213682] Kazemian et al., 2011, Nucleic Acids Res. 39(22): 9463--9472 Improved accuracy of supervised CRM discovery with interpolated Markov models and cross-species comparison. [FBrf0216971] Ohsawa et al., 2011, Dev. Cell 20(3): 315--328 Elimination of Oncogenic Neighbors by JNK-Mediated Engulfment in Drosophila. [FBrf0213219] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...