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Gene Dmel\mbc

General Information
Symbol	Dmel\mbc	Species	D. melanogaster
Name	myoblast city	Annotation symbol	CG10379
Feature type	protein_coding_gene	FlyBase ID	FBgn0015513
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	Dock180, Su(rac)1
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	95B5-95B7	Sequence location	3R:19,607,488..19,627,356 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene myoblast city is referred to in FlyBase by the symbol Dmel\mbc (CG10379, FBgn0015513). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding. There is experimental evidence that it is involved in the biological process: myoblast fusion; dorsal closure; actin cytoskeleton organization; larval visceral muscle development; cell competition in a multicellular organism; cytoskeleton organization. 39 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; anatomical structure; non-connected developing system; embryonic/larval excretory system; organ system subdivision; portion of tissue; embryonic/larval hypodermal muscle; hypodermal muscle of larval abdomen; late extended germ band embryo. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: C2 calcium-dependent membrane targeting; Dedicator of cytokinesis; Src homology-3 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult fat body, inseminated spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1631013_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mbc. Gene sequence location is 3R:19607488..19627356.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms cytoskeleton organization References Knowles-Barley, 2011.8.24, BrainTrap expression curation. Jin et al., 2011, Dev. Cell 20(5): 623--638
FB2012_01	Sequence features HFA16995 BKN28072
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 95B5-95B7   Limits computationally determined from genome sequence between P{PZ}l(3)0468404684 and P{PZ}Atg600096
Experimentally Determined Cytological Location
Cytogenetic map Notes References 95B-95C     (Erickson, 1997.6.10)
Experimentally Determined Recombination Data
Location
Left of (cM)	ca (Nolan et al., 1998)
Right of (cM)	e (Nolan et al., 1998)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mbc for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone SD06708 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mbc-RA FBtr0084459  NM_057796   7350   1970 mbc-RB FBtr0304729     7464   2008
Additional Transcript Data & Comments
Reported size (kB)	7.5 (northern blot) (Erickson et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mbc-PA   FBpp0083850   226.5   1970   7.25     NP_477144   AAF56191 mbc-PB   FBpp0293271   230.7   2008   7.45
Additional Polypeptide Data & Comments
Reported size (kDa)	1970 (aa); 226 (kD predicted) (Erickson et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Src homology-3 domain (IPR001452) C2 calcium-dependent membrane targeting (IPR000008) Dedicator of cytokinesis (IPR010703)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF56191   AC008201   AF007805 AAB69648   AI534176   AQ073771   AY122231 AAM52743   AY122254 AAM52766   BG641089   BI236909   BI368816   BI632794   BI635134   BI639891   BI641814   BP549612   BP549613   BT030856   BT100188   BZ748829   CO154663   CO186018   CO263519   CO269839   CO269854   CO276116   CO293792   CO301007   CO303432   CO306514   CO314098   CO314681   CO321867   CO330952   CO331837   CO333187   CZ468925   CZ469040   CZ469714   CZ469731   CZ469938   CZ488669   EC062517   EC062909   EC067743   EC071233   EC196901   EC205368   EC206177   EC208299   EC217598   EC226055   EC231836   EC232002   EC235823   EC239515   EC248754   EC251894   EC260994   EV579789   EV595074   EV599766   FE863340   GH739205   GH739245   GH740981   GH744989   GH746025   GH764890   GH765951   GH766287   GH767086   GH940833
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8MQW7 Q8MQY7 Q9VCH4
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 12   ectoderm | striped   (Erickson et al., 1997) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Erickson et al., 1997) embryonic stage 1 -- 9     (Erickson et al., 1997) Comment:reference states 0-4 hr AEL embryonic stage 4   pole cell   (Erickson et al., 1997) embryonic stage 5   organism | lateral   (Erickson et al., 1997) embryonic stage 7 -- 12   ectoderm   (Erickson et al., 1997) embryonic stage 12   endoderm   (Erickson et al., 1997) embryonic stage 12 -- 14   somatic mesoderm   (Erickson et al., 1997) embryonic stage 12 -- 17   visceral mesoderm   (Erickson et al., 1997) embryonic stage 14   embryonic/larval visceral muscle   (Erickson et al., 1997) embryonic stage 16   embryonic/larval dorsal vessel   (Erickson et al., 1997) pupal stage     (Erickson et al., 1997) adult stage | female     (Erickson et al., 1997) adult stage | male     (Erickson et al., 1997)
Additional Descriptive Data
	Both the cardial and pericardial cells of the dorsal vessel express mbc transcript. No mbc transcript is detected in mature muscles. (Erickson et al., 1997)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 5   pole cell   (Erickson et al., 1997) embryonic stage 12   endoderm   (Erickson et al., 1997) myoblast   (Erickson et al., 1997) ectoderm   (Erickson et al., 1997) embryonic stage 14   embryonic/larval visceral muscle   (Erickson et al., 1997) embryonic stage 16   embryonic/larval dorsal vessel   (Erickson et al., 1997)
Additional Descriptive Data
	mbc protein expression correlates approximately with mbc transcript expression. Although mbc transcript is not detected in mature muscles, mbc protein is detected at low levels in mature muscles. Colocalization studies with Mef2 protein indicate that mbc protein is present in ectodermal and endodermal germ layers, with ectodermal expression being restricted to the epidermal layer, and that mbc is expressed in myoblasts. (Erickson et al., 1997)
Marker for
Subcellular Localization
CV Term	cytoplasm (Erickson et al., 1997)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0015513    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0015513 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0015513 expression level Developmental Stage   Expression Level embryo 00-02hr    1894 embryo 02-04hr    1957 embryo 04-06hr    4300 embryo 06-08hr    4077 embryo 08-10hr    4002 embryo 10-12hr    2516 embryo 12-14hr    2729 embryo 14-16hr    1486 embryo 16-18hr    1765 embryo 18-20hr    1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new    1758 white prepupae 12hr    1654 white prepupae 24hr    1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day    1416 adult female 05day    1973 adult female 30day    2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1894) embryo 02-04hr  (1957) embryo 04-06hr  (4300) embryo 06-08hr  (4077) embryo 08-10hr  (4002) embryo 10-12hr  (2516) embryo 12-14hr  (2729) embryo 14-16hr  (1486) embryo 16-18hr  (1765) embryo 18-20hr  (1490) embryo 20-22hr  (835) embryo 22-24hr  (1075) larva L1  (766) larva L2  (630) larva L3 12hr old  (623) larva L3 puffstage 1-2  (477) larva L3 puffstage 3-6  (841) larva L3 puffstage 7-9  (1153) white prepupae new  (1758) white prepupae 12hr  (1654) white prepupae 24hr  (1455) pupae 2d postWPP  (1097) pupae 3d postWPP  (982) pupae 4d postWPP  (854) adult male 01day  (1005) adult male 05day  (1017) adult male 30day  (975) adult female 01day  (1416) adult female 05day  (1973) adult female 30day  (2056) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (1894) embryo 02-04hr  (1957) embryo 04-06hr  (4300) embryo 06-08hr  (4077) embryo 08-10hr  (4002) embryo 10-12hr  (2516) embryo 12-14hr  (2729) embryo 14-16hr  1486 embryo 16-18hr  (1765) embryo 18-20hr  1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new  (1758) white prepupae 12hr  (1654) white prepupae 24hr  1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day  1416 adult female 05day  (1973) adult female 30day  (2056) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1894 embryo 02-04hr    1957 embryo 04-06hr    4300 embryo 06-08hr    4077 embryo 08-10hr    4002 embryo 10-12hr    2516 embryo 12-14hr    2729 embryo 14-16hr    1486 embryo 16-18hr    1765 embryo 18-20hr    1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new    1758 white prepupae 12hr    1654 white prepupae 24hr    1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day    1416 adult female 05day    1973 adult female 30day    2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1894 embryo 02-04hr    1957 embryo 04-06hr    4300 embryo 06-08hr    4077 embryo 08-10hr    4002 embryo 10-12hr    2516 embryo 12-14hr    2729 embryo 14-16hr    1486 embryo 16-18hr    1765 embryo 18-20hr    1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new    1758 white prepupae 12hr    1654 white prepupae 24hr    1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day    1416 adult female 05day    1973 adult female 30day    2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0015513 expression level Developmental Stage   Expression Level embryo 00-02hr    1894 embryo 02-04hr    1957 embryo 04-06hr    4300 embryo 06-08hr    4077 embryo 08-10hr    4002 embryo 10-12hr    2516 embryo 12-14hr    2729 embryo 14-16hr    1486 embryo 16-18hr    1765 embryo 18-20hr    1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new    1758 white prepupae 12hr    1654 white prepupae 24hr    1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day    1416 adult female 05day    1973 adult female 30day    2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (1894) embryo 02-04hr  (1957) embryo 04-06hr  (4300) embryo 06-08hr  (4077) embryo 08-10hr  (4002) embryo 10-12hr  (2516) embryo 12-14hr  (2729) embryo 14-16hr  (1486) embryo 16-18hr  (1765) embryo 18-20hr  (1490) embryo 20-22hr  (835) embryo 22-24hr  (1075) larva L1  (766) larva L2  (630) larva L3 12hr old  (623) larva L3 puffstage 1-2  (477) larva L3 puffstage 3-6  (841) larva L3 puffstage 7-9  (1153) white prepupae new  (1758) white prepupae 12hr  (1654) white prepupae 24hr  (1455) pupae 2d postWPP  (1097) pupae 3d postWPP  (982) pupae 4d postWPP  (854) adult male 01day  (1005) adult male 05day  (1017) adult male 30day  (975) adult female 01day  (1416) adult female 05day  (1973) adult female 30day  (2056) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  1894 embryo 02-04hr  1957 embryo 04-06hr  (4300) embryo 06-08hr  (4077) embryo 08-10hr  (4002) embryo 10-12hr  (2516) embryo 12-14hr  (2729) embryo 14-16hr  1486 embryo 16-18hr  1765 embryo 18-20hr  1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new  1758 white prepupae 12hr  1654 white prepupae 24hr  1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day  1416 adult female 05day  1973 adult female 30day  2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1894 embryo 02-04hr    1957 embryo 04-06hr    4300 embryo 06-08hr    4077 embryo 08-10hr    4002 embryo 10-12hr    2516 embryo 12-14hr    2729 embryo 14-16hr    1486 embryo 16-18hr    1765 embryo 18-20hr    1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new    1758 white prepupae 12hr    1654 white prepupae 24hr    1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day    1416 adult female 05day    1973 adult female 30day    2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1894 embryo 02-04hr    1957 embryo 04-06hr    4300 embryo 06-08hr    4077 embryo 08-10hr    4002 embryo 10-12hr    2516 embryo 12-14hr    2729 embryo 14-16hr    1486 embryo 16-18hr    1765 embryo 18-20hr    1490 embryo 20-22hr    835 embryo 22-24hr    1075 larva L1    766 larva L2    630 larva L3 12hr old    623 larva L3 puffstage 1-2    477 larva L3 puffstage 3-6    841 larva L3 puffstage 7-9    1153 white prepupae new    1758 white prepupae 12hr    1654 white prepupae 24hr    1455 pupae 2d postWPP    1097 pupae 3d postWPP    982 pupae 4d postWPP    854 adult male 01day    1005 adult male 05day    1017 adult male 30day    975 adult female 01day    1416 adult female 05day    1973 adult female 30day    2056 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0015513    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0015513 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult fat body, inseminated spermatheca. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0015513 expression level Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body  (542.4) Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca  (640.5) Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0015513 expression level Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body  542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca  640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    207.925 Larval Midgut    215.1 Larval Hindgut    210 Larval Malpighian Tubules    122.3 Larval Fat Body    202.3 Larval Salivary Gland    164.7 Larval Trachea    183.925 Larval Carcass    105.15 Adult Head    213.9 Adult Eye    99.275 Adult Brain    210.7 Adult Thoracic-Abdominal Ganglion    277.7 Adult Crop    215.3 Adult Midgut    312.1 Adult Hindgut    202.4 Adult Malpighian Tubules    260.8 Adult Fat Body    542.4 Adult Salivary Gland    171.5 Adult Heart    243.225 Adult VirginFemale Spermatheca    498.3 Adult InseminatedFemale Spermatheca    640.5 Adult Ovary    428.1 Adult Testis    51.5 Adult Male Accessory Gland    92.9 Adult Carcass    249.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    213.9   NA  Eye    99.275   NA  Brain    210.7   207.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    277.7   NA  Crop    215.3   215.1  Midgut    312.1   210  Hindgut    202.4   122.3  Malpighian Tubules    260.8   202.3  Fat Body    542.4   164.7  Salivary Gland    171.5   NA  Heart    243.225   183.925  Trachea    NA   NA  VirginFemale Spermatheca    498.3   NA  InseminatedFemale Spermatheca    640.5   NA  Ovary    428.1   NA  Testis    51.5   NA  Male Accessory Gland    92.9   105.15  Carcass    249.4 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_30
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015513 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10379-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for mbc Stages(s) 1-3 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal mbcCB04603 lethal | embryonic stage | recessive mbcC1 mbcC2 mbcC3 mbcS4 lethal | recessive mbc1.63 mbc2.35 mbc3.5 mbc4.25 semi-lethal | pupal stage, with Scer\GAL4pnr-MD237 mbcGD6965 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 mbcGD6965 Phenotype manifest in Allele A1-7 dorsal acute muscle 1 mbcTT261 A1-7 dorsal acute muscle 1 | embryonic stage 15 mbcD11.2 axon & ventral nerve cord mbc1.63 mbc4.25 border follicle cell | somatic clone mbcC2 mbcD11.2 chaeta | supernumerary, with Scer\GAL4pnr-MD237 mbcGD6965 circular visceral muscle fiber mbcC1 circular visceral muscle primordium mbcC1 commissure mbc1.63 mbc4.25 embryonic/larval anterior Malpighian tubule mbcC1 embryonic/larval Malpighian tubule mbcC1 embryonic/larval muscle system mbcC1 embryonic/larval somatic muscle mbcC1 mbcC2 mbcC3 mbcF12.7 mbcS4 mbcunspecified embryonic dorsal epidermis mbc1.63 mbc2.35 mbc3.5 mbc4.25 mbcC1 mbcF12.7 embryonic Malpighian tubule mbcunspecified embryonic myoblast mbc1.63 mbc2.35 mbc3.5 mbc4.25 mbcC1 mbcC2 mbcC3 mbcS4 embryonic visceral muscle mbcC1 excretory star cell mbcC1 fusion competent cell | embryonic stage mbcC1 mbcC2 mbcC3 mbcS4 longitudinal connective mbc1.63 mbc4.25 longitudinal muscle mbcC1 mesothoracic tergum, with Scer\GAL4pnr-MD237 mbcGD6965 midgut constriction mbcC1 mbcF12.7 myoblast mbcC1 presumptive embryonic/larval muscle system mbcD11.2 thorax, with Scer\GAL4pnr-MD237 mbcdsRNA.Scer\UAS
Classical Alleles ( 29 )
For All Classical Alleles Show
Allele of mbc Class Mutagen Stocks Known lesion mbcC1 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 1 -- mbcC2 amorphic allele - genetic evidence, loss of function allele ethyl methanesulfonate 1 -- mbcC3 amorphic allele - genetic evidence ethyl methanesulfonate 1 -- mbcc04167 piggyBac transposase 1 -- mbcc05056 piggyBac transposase 1 -- mbcc05087 piggyBac transposase 1 -- mbcc05391 piggyBac transposase 1 -- mbcCB-0487-3 1 -- mbcD11.2 loss of function allele ethyl methanesulfonate 1 Yes mbcEY01437 P-element activity 1 -- mbcf06027 piggyBac transposase 1 -- mbcNP5474 1 -- mbcS4 amorphic allele - genetic evidence ethyl methanesulfonate 0 -- mbc1.63 ethyl methanesulfonate 0 -- mbc2.35 ethyl methanesulfonate 0 -- mbc3.5 ethyl methanesulfonate 0 -- mbc4.25 ethyl methanesulfonate 0 -- mbcc03965 piggyBac transposase 0 -- mbcCB04603 P-element activity 0 Yes mbcCPTI000332 piggyBac transposase 0 Yes mbcCPTI001082 piggyBac transposase 0 Yes mbcEP3135 P-element activity 0 -- mbcEPg36390 P-element activity 0 Yes mbcF12.7 ethyl methanesulfonate 0 Yes mbcF6.4 loss of function allele ethyl methanesulfonate 0 Yes mbcI6.6 ethyl methanesulfonate 0 Yes mbcTT261 0 -- mbcunspecified 0 -- mbczz351 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mbc Class Mutagen Stocks Known lesion mbcGD6965 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbcHMS00346 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbcHMS00604 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mbcAct5C.T:SV5\V5,T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mbcdsRNA.cIa in vitro construct - RNAi 0 Yes mbcdsRNA.Scer\UAS in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mbcMtnA.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mbcScer\UAS.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mbcΔPRM.MtnA.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes mbcΔSH3.MtnA.T:Hsap\MYC,T:Ivir\HA1 in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)BSC489 (Christensen et al., 2008.4.15) Df(3R)Exel9014 (Ryder, 2004.4.26) Df(3R)mbc-15A (Erickson et al., 1997) Df(3R)mbc-30 (Keller et al., 1998, Rushton et al., 1995, Erickson et al., 1997) Df(3R)mbc-BG1 (Presgraves et al., 2003) Df(3R)mbc-F5.3 (Erickson et al., 1997) Df(3R)mbc-R1 (Rushton et al., 1995, Drysdale, 1996.6.25) Df(3R)mbc-R.1 (Erickson et al., 1997) Df(3R)nau-9 (Keller et al., 1998) Df(3R)γ20 (Keller et al., 1998)
Not disrupted in	Df(3R)CA (Keller et al., 1998, Presgraves et al., 2003) Df(3R)CA2 (Erickson et al., 1997) Df(3R)CA15 (Erickson et al., 1997) Df(3R)nau-4A (Keller et al., 1998) Df(3R)nau-11a4 (Keller et al., 1998)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD6965} NA P{TRiP.HMS00346} NA P{TRiP.HMS00604} NA P{UAS-mbc.IR} NA P{UAS-mbc.MYC} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}mbcEP3135 NA P{EPg}mbcEPg36390 NA P{EPgy2}mbcEY01437 NA miscellaneous insertions P{PTT-GB}mbcCB04603 NA P{RS3}mbcCB-0487-3 NA PBac{602.P.SVS-1}mbcCPTI000332 NA PBac{602.P.SVS-1}mbcCPTI001082 NA PBac{PB}mbcc03965 NA PBac{PB}mbcc04167 NA PBac{PB}mbcc05056 NA PBac{PB}mbcc05087 NA PBac{PB}mbcc05391 NA PBac{WH}mbcf06027 NA insertion of enhancer trap binary system P{GawB}mbcNP5474 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with rols (Chen and Olson, 2001)
Biological Process
CV term Evidence References
actin cytoskeleton organization inferred from mutant phenotype (Richardson et al., 2007) cell competition in a multicellular organism inferred from mutant phenotype (Li and Baker, 2007) cytoskeleton organization inferred from mutant phenotype (Erickson et al., 1997) dorsal closure inferred from mutant phenotype (Erickson et al., 1997) larval visceral muscle development inferred from mutant phenotype (Klapper et al., 2002) myoblast fusion inferred from mutant phenotype (Rushton et al., 1995, Menon et al., 2005, Estrada et al., 2006, Richardson et al., 2007)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Erickson et al., 1997)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
GTPase binding inferred from electronic annotation with InterPro:IPR010703 (FlyBase Curators et al., 2004-) GTP binding inferred from electronic annotation with InterPro:IPR010703 (FlyBase Curators et al., 2004-) guanyl-nucleotide exchange factor activity inferred from electronic annotation with InterPro:IPR010703 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
cell morphogenesis traceable author statement (Noselli and Agnes, 1999) dorsal closure traceable author statement (Noselli and Agnes, 1999, Van Aelst and Symons, 2002, Harden, 2002) myoblast fusion traceable author statement (Dworak and Sink, 2002) skeletal muscle tissue development traceable author statement (Cooper, 2001)
Cellular Component
CV term Evidence References
ruffle traceable author statement (Noselli and Agnes, 1999)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mbc allele Gene References mbc1.63 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbc2.35 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbc3.5 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbc4.25 Rac1 (Nolan et al., 1998) Rac2 (Nolan et al., 1998) mbcD11.2 Pvr (Duchek et al., 2001, Ishimaru et al., 2004) mbcEPg36390 slbo (Duchek et al., 2001)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67754 DroID - A comprehensive database of gene and protein interactions. • FBgn0015513 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42817
Orthologs
Genome-wide drosophilid orthologs	Dana\GF16263 Dere\GG11230 Dgri\GH23629 Dmoj\GI23072 Dper\GL23263 Dpse\GA10288 Dsec\GM26532 Dvir\GJ22588 Dwil\GK11109 Dyak\GE10396
Curated drosophilid orthologs	Dpse\GA10288   Dsim\mbc (Landry et al., 2005)
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007589-PA Aedes aegypti (mosquito) AGAP004320-PA Anopheles gambiae (mosquito) XP_001948699.1 Acyrthosiphon pisum (pea aphid) 91300 Branchiostoma floridae (lancelet) ENSBTAP00000032659 Bos taurus (bovine) CAL0002647 Candida albicans (a form of yeast) CN09755 Caenorhabditis brenneri (small nematode) CN29650 Caenorhabditis brenneri (small nematode) CBP34537 Caenorhabditis briggsae (nematode) CE24779 Caenorhabditis elegans (worm) ENSCAFP00000024998 Canis familiaris (dog) ENSCINP00000015199 Ciona intestinalis (sea squirt) JA08686 Caenorhabditis japonica (nematode) cn08369 Cryptococcus neoformans (yeast-like fungus) CPIJ002759 Culex pipens (mosquito) ENSCPOP00000013973 Cavia porcellus (guinea pig) RP30810 Caenorhabditis remanei (nematode) ENSCSAVP00000001882 Ciona savignyi (sedentary tunicate) 126169 Capitella spl (polychaete worm) DEHA2D10890g Debaryomyces Hansenii (yeast) 313508 Daphnia pulex (daphnia) ENSECAP00000021625 Equus caballus (horse) ENSGACP00000003466 Gymnopilus aculeatus (mushroom) ENSGALP00000001117 Gallus gallus (chicken) 109536 Hypsipyla robusta (cedar shoot borer) ENSP00000381107 Homo sapiens (human) ISCW003358-PA Ixodes scapularis (deer tick) KLLA0D02860g Kluyveromyces lactis (yeast) 232874 Lottia gigantea (owl limpet) ENSMODP00000008745 Monodelphis domestica (gray opossum) ENSMMUP00000024404 Macaca mulatta (rhesus monkey) ENSMUSP00000081531 Mus musculus (house mouse) 159083 Nematostella vectensis (starlet anemone) ENSOANP00000022680 Ornithorynchus anatinus (platypus) ENSORLP00000012411 Oryzias latipes (japanese killifish) PHUM002154-PA Pediculus humanus (human body louse) PP03595 Pristionchus pacificus (nematode) ENSPPYP00000017939 Pongo pygmaeus (orangutan) ENSPTRP00000059109 Pan troglodytes (chimpanzee) ENSRNOP00000051781 Rattus norvegicus (norway rat) XP_001197686.1 Strongylocentrotus purpuratus (purple sea urchin) 58664 Trichoplax adhaerens (metazoa) XP_972351.1 Tribolium castaneum (red flour beetle) ENSTNIP00000008097 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000016682 Takifugu rubripes (pufferfish Takifugu) 90729.m00123 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000011369 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13200 NV50059 LH22049 ISCW003358 FBgn0228261 FBgn0213120 FBgn0209695 FBgn0192495 FBgn0181385 JGI_V11_313508 FBgn0070346 FBgn0160853 FBgn0145799 FBgn0131086 FBgn0103531 FBgn0093285 CPIJ002759 BGIBMGA002322 BGIBMGA002321 ACYPI008946 GB16498 AGAP004320 AAEL007589 PHUM252200 GLEAN_12454
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	32355 y1 sc* v1; P{TRiP.HMS00346}attP2 33722 y1 sc* v1; P{TRiP.HMS00604}attP2 4952 st1 e1 mbcD11.2/TM3, Ser1
Harvard	- PBac{PB}mbcc04167 - PBac{PB}mbcc05056 - PBac{PB}mbcc05087 - PBac{PB}mbcc05391 - PBac{WH}mbcf06027
Kyoto	123198 w1118; P{RS3}mbcCB-0487-3 113750 w*; P{GawB}mbcNP5474 / TM6, P{UAS-lacZ.UW23-1}UW23-1 101733 red1 e1 mbcC2 / TM3, Sb1 ry1 e1
VDRC	v16044 w1118; P{GD6965}v16044
	More stocks available...
Genomic Clones ( 1 )
	BACR11E07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 57 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE54856 SD06708
Other clones	IP18282 MIP14651
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE33039 SD12332 SD16380 SD22594 SD25447 SD26666
Other clones	54667 60264 118205 121308 193668 214219.15 274333 281364 523988 CK01.13D.D6 CK01.15A.F7 CK01.106B.F7 CK01.125B.G6 CK01.129D.G1 CK01.145D.F9 Dmel_dig_012417_0691_1861 Dmel_neb_006474_0880_0389 Dmel_neb_010767_0707_0398 EC235823 EK015224 EK088724 EK096045 EK096125 EK119723 EK179864 EK186561 EK204837 EK235108 EK237427 EK241439 EK251304 EK296637 EK301816 EK306140 EN04546 FGM102D11 RM07042 RP001011381 RP001012227 RP001017550 RP001018205 RP001019162 RP001031023 RP001042649 RP001043012 RP001054177 RP003651652
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015513 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42817
Antibody Information
	polyclonal (Erickson et al., 1997)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	mbc is needed in wild-type cells for the death of Minute neighbours in cell competition experiments. (Li and Baker, 2007) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003) mbc is required for the formation of syncytia within the visceral musculature of the midgut. (Klapper et al., 2002) The mbc product functions as a specific upstream regulator of Rac1 activity that mediates several morphogenetic processes in embryogenesis. (Nolan et al., 1998) Light and electron microscopy are used to study the process of myoblast fusion in mbc mutants. (Doberstein et al., 1997) Neuromuscular junctions (NMJ) can form on founder cells in the absence of myoblast fusion these NMJs have morphological and physiological properties closely resembling those of wild type. A smaller surface area of the fibre than wild type is devoted to the NMJ. (Prokop et al., 1996) mbc is required for the fusion of myoblasts into multinucleate muscles. The phenotype of mbc mutants provides evidence in support of the founder cell hypothesis of muscle development. (Rushton et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057796 RefSeq (Proteins) • NP_477144 DDBJ / EMBL / GenBank • AAB69648 AAF56191 AAM52743 AAM52766 AC008201 AF007805 AI534176 AQ073771 AY122231 AY122254 BI368816 BZ748829 CZ468925 CZ469040 CZ469714 CZ469731 CZ469938 CZ488669 Entrez Gene - A searchable database of RefSeq genes. • 42817 UniProtKB/TrEMBL • Q8MQW7 Q8MQY7 Q9VCH4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Src homology-3 domain (IPR001452) C2 calcium-dependent membrane targeting (IPR000008) Dedicator of cytokinesis (IPR010703)
Linkouts
	BioGRID - A database of protein and genetic interactions • 67754 DroID - A comprehensive database of gene and protein interactions. • FBgn0015513 DRSC - Results from RNAi screens. • FBgn0015513 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015513 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10379-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015513 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42817 InParanoid A subset of ortholog calls from InParanoid. • AAEL007589-PA Aedes aegypti (mosquito) AGAP004320-PA Anopheles gambiae (mosquito) XP_001948699.1 Acyrthosiphon pisum (pea aphid) 91300 Branchiostoma floridae (lancelet) ENSBTAP00000032659 Bos taurus (bovine) CAL0002647 Candida albicans (a form of yeast) CN09755 Caenorhabditis brenneri (small nematode) CN29650 Caenorhabditis brenneri (small nematode) CBP34537 Caenorhabditis briggsae (nematode) CE24779 Caenorhabditis elegans (worm) ENSCAFP00000024998 Canis familiaris (dog) ENSCINP00000015199 Ciona intestinalis (sea squirt) JA08686 Caenorhabditis japonica (nematode) cn08369 Cryptococcus neoformans (yeast-like fungus) CPIJ002759 Culex pipens (mosquito) ENSCPOP00000013973 Cavia porcellus (guinea pig) RP30810 Caenorhabditis remanei (nematode) ENSCSAVP00000001882 Ciona savignyi (sedentary tunicate) 126169 Capitella spl (polychaete worm) DEHA2D10890g Debaryomyces Hansenii (yeast) 313508 Daphnia pulex (daphnia) ENSECAP00000021625 Equus caballus (horse) ENSGACP00000003466 Gymnopilus aculeatus (mushroom) ENSGALP00000001117 Gallus gallus (chicken) 109536 Hypsipyla robusta (cedar shoot borer) ENSP00000381107 Homo sapiens (human) ISCW003358-PA Ixodes scapularis (deer tick) KLLA0D02860g Kluyveromyces lactis (yeast) 232874 Lottia gigantea (owl limpet) ENSMODP00000008745 Monodelphis domestica (gray opossum) ENSMMUP00000024404 Macaca mulatta (rhesus monkey) ENSMUSP00000081531 Mus musculus (house mouse) 159083 Nematostella vectensis (starlet anemone) ENSOANP00000022680 Ornithorynchus anatinus (platypus) ENSORLP00000012411 Oryzias latipes (japanese killifish) PHUM002154-PA Pediculus humanus (human body louse) PP03595 Pristionchus pacificus (nematode) ENSPPYP00000017939 Pongo pygmaeus (orangutan) ENSPTRP00000059109 Pan troglodytes (chimpanzee) ENSRNOP00000051781 Rattus norvegicus (norway rat) XP_001197686.1 Strongylocentrotus purpuratus (purple sea urchin) 58664 Trichoplax adhaerens (metazoa) XP_972351.1 Tribolium castaneum (red flour beetle) ENSTNIP00000008097 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000016682 Takifugu rubripes (pufferfish Takifugu) 90729.m00123 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000011369 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/myblcy1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42817 modMine - Data generated by the modENCODE project. • FBgn0015513 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13200 NV50059 LH22049 ISCW003358 FBgn0228261 FBgn0213120 FBgn0209695 FBgn0192495 FBgn0181385 JGI_V11_313508 FBgn0070346 FBgn0160853 FBgn0145799 FBgn0131086 FBgn0103531 FBgn0093285 CPIJ002759 BGIBMGA002322 BGIBMGA002321 ACYPI008946 GB16498 AGAP004320 AAEL007589 PHUM252200 GLEAN_12454
Synonyms & Secondary IDs ( 13 )
Reported As
Symbol Synonym	CG10379 (Rogers et al., 2003, Wittkopp et al., 2008, Ni et al., 2010.12.1) Dm MBC (Meller et al., 2005) Dock180 (Meller et al., 2005) DOCK180 (Ridley, 2001) dock180 (McPhee et al., 2010) Mbc (Landry et al., 2005, Ishimaru et al., 2004, Kockel et al., 2001, Chen et al., 2007, Meller et al., 2005) MBC (Donaldson, 2003) mbc (Geisbrecht et al., 2007, Menon et al., 2005, Estrada et al., 2006, Buszczak et al., 2007, Li and Baker, 2007, Arancio et al., 2010, Christensen et al., 2008.4.15, Puetz et al., 2005, Bakal et al., 2007, Ohsawa et al., 2011, Balagopalan et al., 2006, Kesper et al., 2007, Franklin-Dumont et al., 2007, Borghese et al., 2006, Kim et al., 2007, Massarwa et al., 2007, Richardson et al., 2007, Estrada et al., 2007, Beckett and Baylies, 2007, Geisbrecht et al., 2008, Chen et al., 2004, Paladi and Tepass, 2004, Liu et al., 2009, Shelton et al., 2009, Wolfstetter et al., 2009, Zeitlinger et al., 2007, Wittkopp et al., 2008, Kuraishi et al., 2009, Bulchand et al., 2010, McPhee et al., 2010, Jin et al., 2011) MBC/DOCK180 (Li and Baker, 2007) Su(rac)1 (Stronach and Perrimon, 1999, Nolan et al., 1998)
Name Synonym	Dock180 (Bulchand et al., 2010) Myoblast City (Donaldson, 2003) myoblast city (Rushton et al., 1995, Menon et al., 2005, Meller et al., 2005, Balagopalan et al., 2006, Chen et al., 2004, Paladi and Tepass, 2004, Wolfstetter et al., 2009, McPhee et al., 2010, Jin et al., 2011) Myoblast city (Geisbrecht et al., 2007, Cho et al., 2005, Rogers et al., 2003, Chen et al., 2007, Puetz et al., 2005, Geisbrecht et al., 2008, Bulchand et al., 2010)
Secondary FlyBase IDs
References ( 139 )
Generate a list of	All references relating to this gene ( 139 )
List References by type	Research paper  ( 62 ) Supplementary material  ( 9 ) Review  ( 34 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 18 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Computer file  ( 3 ) Automatic genome annotation  ( 1 )
Recent research papers ( 6 )
	Jin et al., 2011, Dev. Cell 20(5): 623--638 Competition between Blown Fuse and WASP for WIP Binding Regulates the Dynamics of WASP-Dependent Actin Polymerization In Vivo. [FBrf0213682] Ohsawa et al., 2011, Dev. Cell 20(3): 315--328 Elimination of Oncogenic Neighbors by JNK-Mediated Engulfment in Drosophila. [FBrf0213219] Arancio et al., 2010, Genetics 185(1): 129--140 The nucleosome remodeling factor ISWI functionally interacts with an evolutionarily conserved network of cellular factors. [FBrf0213976] Bulchand et al., 2010, PLoS ONE 5(2): e9374 The intracellular domain of dumbfounded affects myoblast fusion efficiency and interacts with rolling pebbles and loner. [FBrf0210103] Lesch et al., 2010, Genetics 186(3): 943--957 A Targeted UAS-RNAi Screen in Drosophila Larvae Identifies Wound Closure Genes Regulating Distinct Cellular Processes. [FBrf0212282] McPhee et al., 2010, Nature 465(7301): 1093--1096 Activation of autophagy during cell death requires the engulfment receptor Draper. [FBrf0211145] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...