a docking protein: DOCK180 homolog - by guanine nucleotide exchange factor - essential for myoblast fusion - in combination with Ced-12, activates the Rac1 monomeric GTPase - contributes to border cell migration and dorsal closure
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
None of the polypeptides share 100% sequence identity.
1970 (aa); 226 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mbc using the Feature Mapper tool.
mbc protein expression correlates approximately with mbc transcript expression. Although mbc transcript is not detected in mature muscles, mbc protein is detected at low levels in mature muscles. Colocalization studies with Mef2 protein indicate that mbc protein is present in ectodermal and endodermal germ layers, with ectodermal expression being restricted to the epidermal layer, and that mbc is expressed in myoblasts.
GBrowse - Visual display of RNA-Seq signalsView Dmel\mbc in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
mbc is both necessary and sufficient in the fusion competent myoblasts for fusion with founder cells during embryonic somatic muscle development.
mbc is needed in wild-type cells for the death of Minute neighbours in cell competition experiments.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
mbc is required for the formation of syncytia within the visceral musculature of the midgut.
Light and electron microscopy are used to study the process of myoblast fusion in mbc mutants.
Neuromuscular junctions (NMJ) can form on founder cells in the absence of myoblast fusion these NMJs have morphological and physiological properties closely resembling those of wild type. A smaller surface area of the fibre than wild type is devoted to the NMJ.