FB2022_02, released March 29, 2022

Gene: Dmel\RpS21

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General Information
Symbol
Dmel\RpS21
Species
D. melanogaster
Name
Ribosomal protein S21
Annotation Symbol
CG2986
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015521
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Summary
May be an associated component of the ribosome rather than a core structural subunit. May act as a translation initiation factor. Has a role in regulation of cell proliferation in the hematopoietic organs and the imaginal disks of larva. (UniProt, O76927)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

oho23B, l(2)168/14, l(2)k16814, oho23, overgrown hematopoietic organs at 23B

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-7
RefSeq locus
NT_033779 REGION:2856070..2857029
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
enables ribosome binding
inferred from physical interaction with UniProtKB:P38979
(assigned by UniProt )
(Torok et al., 1999)
enables structural constituent of ribosome
inferred from high throughput direct assay
(Alonso and Santaren, 2006)
inferred from direct assay
(Anger et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables structural constituent of ribosome
traceable author statement
(Marygold et al., 2007)
inferred from biological aspect of ancestor with PANTHER:PTN000047682
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (6 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in lymph gland development
inferred from mutant phenotype
(Torok et al., 1993)
involved_in regulation of cell population proliferation
inferred from mutant phenotype
(assigned by UniProt )
(Torok et al., 1999)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in cytoplasmic translation
inferred by curator from GO:0022626
(Anger et al., 2013, Alonso and Santaren, 2006)
traceable author statement
(Marygold et al., 2007)
involved_in endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
inferred from biological aspect of ancestor with PANTHER:PTN000047682
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
inferred from biological aspect of ancestor with PANTHER:PTN000047682
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in translation
inferred from electronic annotation with InterPro:IPR001931, InterPro:IPR018279
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytosolic ribosome
inferred from direct assay
(Anger et al., 2013)
inferred from high throughput direct assay
(Alonso and Santaren, 2006)
located_in ribosome
inferred from direct assay
(assigned by UniProt )
(Torok et al., 1999)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of cytosolic small ribosomal subunit
inferred from biological aspect of ancestor with PANTHER:PTN000047682
(assigned by GO_Central )
(Gaudet et al., 2011)
traceable author statement
(Marygold et al., 2007)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the eukaryotic ribosomal protein eS21 family. (O76927)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
CYTOPLASMIC SMALL RIBOSOMAL PROTEINS -
Cytoplasmic ribosomes are found on the endoplasmic reticulum and in the cytoplasm. They translate all mRNAs produced from nuclear genes and perform the majority of cellular protein synthesis. Ribosomes are formed by two ribonucleoprotein subunits, large and small. The principle function of the small subunit is to bind mRNA. (Adapted from FBrf0205398)
Protein Function (UniProtKB)
May be an associated component of the ribosome rather than a core structural subunit. May act as a translation initiation factor. Has a role in regulation of cell proliferation in the hematopoietic organs and the imaginal disks of larva.
(UniProt, O76927)
Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\RpS21 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O76927)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
RpS21-RA
FBtr0077621
NM_164506
391
83
RpS21-RB
FBtr0077623
NM_164507
619
83
RpS21-RD
FBtr0077622
NM_164508
415
83
RpS21-RE
FBtr0273293
NM_078738
494
81
RpS21-RF
FBtr0335131
NM_001273041
715
83
Additional Transcript Data and Comments
Reported size (kB)

0.7, 0.4 (northern blot)

(Torok et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
RpS21-PA
FBpp0077307
9.2
83
5.69
NP_722853
AAF51191
RpS21-PB
FBpp0077309
9.2
83
5.69
NP_722854
AAN10392
RpS21-PD
FBpp0077308
9.2
83
5.69
NP_722855
AAN10394
RpS21-PE
FBpp0271801
8.9
81
5.69
NP_523462
AAN10393
RpS21-PF
FBpp0307130
9.2
83
5.69
NP_001259970
AGB92507
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

83 aa isoforms: RpS21-PA, RpS21-PB, RpS21-PD, RpS21-PF
Additional Polypeptide Data and Comments
Reported size (kDa)

83 (aa); 9.3 (kD predicted)

(Torok et al., 1999)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the 40S small ribosomal subunit (PubMed:23636399). Interacts with sta (PubMed:10022917).

(UniProt, O76927)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RpS21 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Torok et al., 1999)
Additional Descriptive Data

oho23B transcripts are expressed fairly uniformly throughout development.

(Torok et al., 1999)

0.7kb oho23B transcripts are expressed at an abundance of less than 5% of the 0.4kb transcripts.

(Torok et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Torok et al., 1999)
Additional Descriptive Data

oho23B protein is relatively uniformly expressed at all developmental stages.

(Torok et al., 1999)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosolic ribosome
inferred from direct assay
(Anger et al., 2013)
inferred from high throughput direct assay
(Alonso and Santaren, 2006)
located_in ribosome
inferred from direct assay
(assigned by UniProt )
(Torok et al., 1999)
Expression Deduced from Reporters
Reporter: P{PZ}RpS2103575
Stage
Tissue/Position (including subcellular localization)
Reference
wandering third instar larval stage
imaginal disc
(Meister and Braun, 1995.10)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RpS21 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 7 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RpS21
Transgenic constructs containing regulatory region of RpS21
Aberrations (Deficiencies and Duplications) ( 11 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\RPS21
14 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Rps21
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Rps21
10 of 13
Yes
Yes
Rnor\Rps21-ps1
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\rps21
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (1)
Drer\rps21
13 of 15
Yes
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\rps-21
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\AT3G53890
9 of 9
Yes
Yes
Atha\AT5G27700
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\RPS21B
13 of 15
Yes
Yes
Scer\RPS21A
11 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\rps21
11 of 12
Yes
Yes
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
RpS21k16804a
exacerbates  tauopathy
modeled by Hsap\MAPTGMR.Ex.PJ
(Ambegaokar and Jackson, 2011)
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
RPS21; ribosomal protein S21
14 of 15
180477
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      RpS21 - Arc1
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - BthD
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG1129
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG11777
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG1218
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG14036
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG14715
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG1532
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG18081
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG18787
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG18789
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG31142
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG6171
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - CG7049
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Ccs
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Fkbp12
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Got1
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Hr39
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Hsf
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Rack1
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - Rae1
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - RpL10
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL10Ab
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL11
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL12
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL13
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL13A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL14
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL15
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL17
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL18
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL18A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL19
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL21
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL22
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL23
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL23A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL24
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL26
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL27
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL27A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL28
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL29
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL3
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL30
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL31
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL32
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL34a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL35
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL35A
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL36
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL36A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL37A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL37a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL38
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL39
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL4
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL40
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL41
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL5
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL6
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL7
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL7A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL8
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpL9
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpLP0
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpLP1
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpLP2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS10a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS11
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS12
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS13
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS14a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS14b
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS15
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS15Aa
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS16
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS17
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS18
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS19a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS20
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS23
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS24
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS25
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS26
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS27
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS27A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS28b
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS29
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS3
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS30
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS3A
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS4
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS5a
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS6
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS7
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS8
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - RpS9
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - Sh3β
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Sod1
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Treh
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Trx-2
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - Wdr82
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - awd
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - cib
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - eEF2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - regucalcin
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - sta
      pull down, autoradiography, cosedimentation, western blot, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013, Torok et al., 1999)
      RpS21 - step
      experimental knowledge based
      (Guruharsha et al., 2011)
      RpS21 - vig2
      cosedimentation, Identification by mass spectrometry, electron microscopy
      (Anger et al., 2013)
      RpS21 - αTry
      experimental knowledge based
      (Guruharsha et al., 2011)
      RNA-protein
      Physical Interaction
      Assay
      References
      RpS21 - 18SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - 28SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - 2SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - 5.8SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      RpS21 - 5SrRNA
      cosedimentation, electron microscopy
      (Anger et al., 2013)
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      RpS21
      enhanceable
      sta
      (Torok et al., 1999)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Bx
      enhanceable
      RpS21
      (Bronstein et al., 2010)
      Pi3K92E
      enhanceable
      RpS21
      (Coelho et al., 2005)
      sta
      enhanceable
      RpS21
      (Torok et al., 1999)
      wg
      suppressible
      RpS21
      (McClure and Schubiger, 2008)
      External Data
      Subunit Structure (UniProtKB)
      Component of the 40S small ribosomal subunit (PubMed:23636399). Interacts with sta (PubMed:10022917).
      (UniProt, O76927 )
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-7
      Cytogenetic map
      23B5-23B6
      Sequence location
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      23B5-23B6
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      23B5-23B6
      (determined by in situ hybridisation)
      (Spradling et al., 1999)
      23B-23B
      (determined by in situ hybridisation)
      (Herrmann et al., 1995, Torok et al., 1993)
      23B5-23B6
      (BDGP Project Members, 1994-1999)
      Experimentally Determined Recombination Data
      Location

      2-7

      (FlyBase, 2016)

      2-6.5

      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (9)
      Genomic Clones (14)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (280)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Torok et al., 1999)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: RpS21 oho23B

      (Torok et al., 1999)
      Source for database merge of
      Additional comments
      Other Comments

      Minute gene.

      (Marygold et al., 2007)

      Deletions removing oho23B but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.

      (Marygold et al., 2007)

      Molecularly-defined mutations in oho23B result in Minute phenotypes.

      (Marygold et al., 2007)

      oho23B acts as a translation initiation factor, rather than as a core ribosomal protein.

      (Torok et al., 1999)

      Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

      (Roch et al., 1998)

      The tumour suppressor gene oho23B encodes a homologue of the human S21 ribosomal protein.

      (Herrmann et al., 1995)

      Mutants display a blood cell neoplastic phenotype.

      (Mechler, 1994)

      Genetic and developmental characteristics of the lymph gland overgrowth mutant have been determined.

      (Torok et al., 1993)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 60 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
      EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
      Flygut - An atlas of the Drosophila adult midgut
      FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      MARRVEL_MODEL - MARRVEL (model organism gene)
      modMine - A data warehouse for the modENCODE project
      Linkouts
      ApoDroso - Functional genomic database for photoreceptor development, survival and function
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (16)
      Datasets (8)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      Study result (8)
      Result
      Result Type
      Title
      scRNAseq_2020_Cattenoz_NI_seq_clustering
      Clustering analysis of hemocytes from non-infested third instar larvae
      scRNAseq_2020_Cattenoz_WI_seq_clustering
      Clustering analysis of hemocytes from wasp-infested third instar larvae
      scRNAseq_2020_Cho_NI72LG_seq_clustering
      Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
      scRNAseq_2020_Cho_NI96LG_seq_clustering
      Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
      scRNAseq_2020_Cho_NI120LG_seq_clustering
      Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
      scRNAseq_2020_Cho_WI96LG_seq_clustering
      Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
      scRNAseq_2020_Cho_NI96HL_seq_clustering
      Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
      scRNAseq_2020_Cho_NI120HL_seq_clustering
      Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
      References (73)