FlyBase Gene Report: Dmel\tty

TWEETY FAMILY -: The Tweety family encode putative large-conductance chloride channels. Tweety proteins are predicted to possess five or six transmembrane domains. (Adapted from FBrf0192351).

Protein Function (UniProtKB)

Non-essential protein that probably acts as a chloride channel.

(UniProt, Q9U6L4)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\tty or the JBrowse view of Dmel\tty for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.55

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.44

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

tty-RA

FBtr0077224

NM_080712

3927

970

tty-RC

FBtr0329900

NM_001272838

3078

542

tty-RD

FBtr0329901

NM_167744

7199

970

tty-RE

FBtr0329902

NM_001272839

3853

966

tty-RF

FBtr0329903

NM_001272840

6642

966

tty-RG

FBtr0333749

NM_001272837

3757

967

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

tty-PA

FBpp0076919

105.8

970

8.19

NP_542443

AAF50831

tty-PC

FBpp0302934

59.7

542

8.77

NP_001259767

AGB95605

tty-PD

FBpp0302935

105.8

970

8.19

NP_728415

AAN09675

tty-PE

FBpp0302936

105.4

966

8.19

NP_001259768

AGB95606

tty-PF

FBpp0302937

105.4

966

8.19

NP_001259769

AGB95607

tty-PG

FBpp0305891

105.5

967

8.19

NP_001259766

AGB95604

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

970 aa isoforms: tty-PA, tty-PD

966 aa isoforms: tty-PE, tty-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

836 (aa)

(Maleszka et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Tweety

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\tty using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (6 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

chloride channel activity

inferred from sequence or structural similarity with UniProtKB:Q9C0H2

(assigned by UniProt )

(UniProt Curators, 2002-)

inferred from sequence or structural similarity with HGNC:22222

(Suzuki, 2006)

intracellular calcium activated chloride channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000965383

(assigned by GO_Central )

(Gaudet et al., 2011)

volume-sensitive chloride channel activity

inferred from biological aspect of ancestor with PANTHER:PTN000965383

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

chloride transport

inferred from sequence or structural similarity with HGNC:22222

(Suzuki, 2006)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

integral component of membrane

inferred from sequence model

(Suzuki, 2006)

plasma membrane

inferred from sequence or structural similarity with UniProtKB:Q9C0H2

(assigned by UniProt )

(UniProt Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000965383

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\tty in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\tty

View exonic expression by tissue for Dmel\tty

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015558

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1693-RB;CG1693-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1693

Flygut - An atlas of the Drosophila adult midgut

FBgn0015558

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of tty

Transgenic constructs containing regulatory region of tty

Deletions and Duplications ( 4 )

Disrupted in

Df(1)16-129

(Maleszka et al., 1996)

Df(1)17-257

(Maleszka et al., 1996)

Df(1)GA104

(Maleszka et al., 1996)

Not disrupted in

Df(1)2/19B

(Maleszka et al., 1996)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

tty^GD3710

viable, with Scer\GAL4^Mef2.PR

tty^GD3710

viable, with Scer\GAL4^pnr-MD237

tty^GD3710

viable, with Scer\GAL4^tin.CΔ4

tty^GD3710

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\TTYH3

11 of 15

Yes

Hsap\TTYH1

10 of 15

Hsap\TTYH2

10 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (3)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ttyh3

11 of 15

Yes

Mmus\Ttyh1

10 of 15

Mmus\Ttyh2

10 of 15

Yes

Rattus norvegicus (Norway rat) (3)

Rnor\Ttyh1

8 of 13

Yes

Rnor\Ttyh3

8 of 13

Yes

Rnor\Ttyh2

5 of 13

Yes

Xenopus tropicalis (Western clawed frog) (3)

Xtro\ttyh1

8 of 12

Yes

Xtro\ttyh2

8 of 12

Yes

Xtro\ttyh3

7 of 12

Yes

Danio rerio (Zebrafish) (5)

Drer\ttyh3b

11 of 15

Yes

Drer\ttyh2

10 of 15

Yes

Drer\ttyh2l

9 of 15

Drer\ttyh3a

9 of 15

Yes

Drer\ttyh1

7 of 15

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\ttyh-1

9 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091903R4 )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\tty

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14222

Drosophila persimilis

Dper\GL19902

Drosophila willistoni

Dwil\GK19929

Drosophila virilis

Dvir\GJ19022

Drosophila mojavensis

Dmoj\GI15862

Drosophila grimshawi

Dgri\GH12408

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915063V )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17024262

Glossina morsitans

Tsetse fly

Gmor\GMOY008246

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_08561

Mayetiola destructor

Hessian fly

Mdes\Mdes005224

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL010941

Anopheles darlingi

American malaria mosquito

Adar\ADAC000562

Anopheles gambiae

Malaria mosquito

Agam\AGAP000860

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003195

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ003194

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0BMV )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007141

Danaus plexippus

Monarch butterfly

Dple\DPOGS210825-PA

Apis florea

Little honeybee

Aflo\3328217188

Apis mellifera

Western honey bee

Amel\LOC413358

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP21671

Bombus terrestris

Buff-tailed bumblebee

Bter\15209579

Linepithema humile

Argentine ant

Lhum\LH21393

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001276

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004462

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12717

Tribolium castaneum

Red flour beetle

Tcas\TC033993

Pediculus humanus

Human body louse

Phum\PHUM092770

Rhodnius prolixus

Kissing bug

Rpro\RPRC009248

Rhodnius prolixus

Kissing bug

Rpro\RPRC009251

Rhodnius prolixus

Kissing bug

Rpro\RPRC009249

Cimex lectularius

Bed bug

Clec\CLEC000389

Cimex lectularius

Bed bug

Clec\CLEC000389-PB

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002050

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05030

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0BKH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR015255

Ixodes scapularis

Black-legged tick

Isca\ISCW015482

Stegodyphus mimosarum

African social velvet spider

Smim\KFM79975.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur05g01240

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_314365

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0IBL )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Ciona intestinalis

Vase tunicate

Cint\LOC100183845

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

6 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

TTYH3; tweety family member 3

11 of 15

608919

TTYH1; tweety family member 1

10 of 15

605784

TTYH2; tweety family member 2

10 of 15

608855

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0015558

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33109

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Stimuli-sensing channels

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-65

Cytogenetic map

19F4-19F5

Sequence location

X:21,328,613..21,339,676 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19F4-19F5

Limits computationally determined from genome sequence between P{EP}CG1702^EP1525 and P{EP}EP1465&P{EP}CG1486^EP1192

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

19F-19F

(determined by in situ hybridisation)

(Campbell et al., 1993)

Deduced from proximity to fliI.

(Campbell et al., 1993)

Experimentally Determined Recombination Data

Location

1-65

(FlyBase, 2016)

1-66.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

57910

y¹ w^* Mi{MIC}tty^MI12269

c03142

v110431

v43841

w¹¹¹⁸; P{GD3710}v43841

Genomic Clones (9)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (30)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD23194

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015558

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015558

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33109

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: tty CG1693

(Li, 1999.11)

Source for database merge of

Additional comments

Other Comments

Defined as a transcription unit during the molecular analysis of the nearby fliI gene.

(Campbell et al., 1993)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33109

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9U6L4

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015558

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1693

Flygut - An atlas of the Drosophila adult midgut

FBgn0015558

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33109

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC033993

InterPro - A database of protein families, domains and functional sites

Tweety

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1693

modMine - A data warehouse for the modENCODE project

FBgn0015558

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0015558

DRSC - Results frm RNAi screens

FBgn0015558

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015558

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1693-RB;CG1693-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015558

FlyMine - An integrated database for Drosophila genomics

FBgn0015558

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33109

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Stimuli-sensing channels

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG1693

(Keleman et al., 2009.8.5, Mount and Mookherjee, 2000.12.14)

TTYH1

(Suzuki, 2006)

tty

(Sarov et al., 2016, Levis et al., 2011.12.16, Buffin and Gho, 2010, Jumbo-Lucioni et al., 2010)

twe

(Maleszka et al., 1996)

Name Synonyms

tweety

(Buffin and Gho, 2010, Suzuki, 2006, Campbell et al., 1993)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (39)

Report Sections

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