unp, upg
homeodomain protein - involved in tracheal branching morphogenesis by regulating cell migration or extension; in the absence of such function, the tracheal founder cells either die or adopt other branch patterns
Please see the GBrowse view of Dmel\unpg or the JBrowse view of Dmel\unpg for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
Gene model reviewed during 5.49
1.8 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
485 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unpg using the Feature Mapper tool.
Comment: maternally deposited
unpg transcripts are first detected in 4-8hr embryos and are expressed throughout embryogenesis. Levels decrease during larval stages and then increase during pupal and adult stages. unpg tanscripts are first detected by in situ at stage 8 in a subset of neuroblasts of the midline. These cells divide to generate neuroblasts and neurons of the CNS that largely correspond to the en-expressing cells. By stage 14, CNS expression is restricted to a few cells per segment. Outside of the CNS, expression is first observed in two clusters of ectodermal cells located laterally with the labial and first thoracic segments at stage 9. By stage 11, expression is seen in 15-20 cells around the anterior part of the first tracheal pit. By stage 13, expression is detected in a few cells close to the dorsal midline. These cells appear to form long cytoplasmic branches that prefigure the cerebral branches of the tracheal system. As the germband retracts, a new expression domain is seen within the invaginated tracheal pits in segments T1-A7. During germband retraction, these precursor cells extend ventrally and dorsally. By stage 14, the ganglionic branch in each hemisegment consists of 7-9 unpg-expressing cells whose cell bodies appear to form a continuous chain that penetrates the CNS of stage 14 embryos. Double labeling experiments show that unpg expression outside of the CNS is limited to cells of the cerebral and ganglionic branches of the tracheal system during embryonic development.
The protein and transcript expression patterns of unpg are indistinguishable. unpg protein is first detected at stage 8 in a subset of neuroblasts of the midline. These cells divide to generate neuroblasts and neurons of the CNS that largely correspond to the en-expressing cells. By stage 14, CNS expression is restricted to a few cells per segment. Outside of the CNS, expression is first observed in two clusters of ectodermal cells located laterally with the labial and first thoracic segments at stage 9. By stage 11, expression is seen in 15-20 cells around the anterior part of the first tracheal pit. By stage 13, expression is detected in a few cells close to the dorsal midline. These cells appear to form long cytoplasmic branches that prefigure the cerebral branches of the tracheal system. As the germband retracts, a new expression domain is seen within the invaginated tracheal pits in segments T1-A7. During germband retraction, these precursor cells extend ventrally and dorsally. By stage 14, the ganglionic branch in each hemisegment consists of 7-9 unpg-expressing cells whose cell bodies appear to form a continuous chain that penetrates the CNS of stage 14 embryos. Double labeling experiments show that unpg expression outside of the CNS is limited to cells of the cerebral and ganglionic branches of the tracheal system during embryonic development.
Comment: late stage 11
Comment: late stage 11
Comment: late stage 11
Comment: late stage 11
Comment: late stage 11
Comment: late stage 11
GBrowse - Visual display of RNA-Seq signals
View Dmel\unpg in GBrowse 22-60
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: unpg CG1650
unpg is a regulatory target for genes of the bithorax complex and is required for formation of the tracheal branches that penetrate the CNS.
cas, eve, unpg and ac are expressed in specific neuroblast sublineages. Expression studies using pbl and stg mutants suggest that neuroblasts have an intrinsic gene regulatory hierarchy controlling unpg and ac expression but that cell cycle- or cytokinesis-dependent mechanisms are required for cas and eve CNS expression.
Expression analysed in CNS study of neuroblasts and ganglion mother cells, using an enhancer trap to reveal the expression pattern.
