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Gene Dmel\α-Est3

General Information
Symbol	Dmel\α-Est3	Species	D. melanogaster
Name	α-Esterase-3	Annotation symbol	CG1257
Feature type	protein_coding_gene	FlyBase ID	FBgn0015571
Gene Model Status	Current	Stock availability	6 publicly available
Also Known As	αE3, alpha-Est3, α-Ests, DmαE3
Genomic Location
Chromosome (arm)	3R	Recombination map	3-
Cytogenetic map	84D9-84D9	Sequence location	3R:3,364,694..3,367,353 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene α-Esterase-3 is referred to in FlyBase by the symbol Dmel\α-Est3 (CG1257, FBgn0015571). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: carboxylesterase activity. The biological processes in which it is involved are not known. 5 alleles are reported. No phenotypic data is available. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Carboxylesterase, type B. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval/adult Malpighian tubules, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, adult midgut, larval/adult hindgut, adult heart, larval/adult fat body, adult salivary gland, adult spermathecae, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625354_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of alpha-Est3. Gene sequence location is 3R:3364694..3367353.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 84D9-84D9   Limits computationally determined from genome sequence between P{EP}EP3060EP3060 and P{PZ}grn05930
Experimentally Determined Cytological Location
Cytogenetic map Notes References 84D8-84D10   (determined by in situ hybridisation)   (Ranz et al., 2001) 84D3-84D10     (Robin, 1996.3.11) 84D3-84D10   (determined by in situ hybridisation)   (Russell et al., 1996)
Experimentally Determined Recombination Data
Location	3- (Robin, 1996.3.11, )
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\α-Est3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.43 Gene model reviewed during 5.49
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) α-Est3-RA FBtr0081545  NM_079543   1825   543 α-Est3-RB FBtr0300496  NM_001170069   1834   543 α-Est3-RC FBtr0310527  NM_001275415   1878   99 α-Est3-RD FBtr0339708     1726   543
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein α-Est3-PA   FBpp0081069   61.4   543   6.79     NP_524267   AAF54004 α-Est3-PB   FBpp0289723   61.4   543   6.79     NP_001163540   ACZ94837 α-Est3-PC   FBpp0302664   11.2   99   5.02     NP_001262344   AGB95727 α-Est3-PD   FBpp0308765   61.4   543   6.79
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Carboxylesterase, type B (IPR002018)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014297 AAF54004   AE014297 ACZ94837   AE014297 AGB95727   AI293962   AI403253   AW944073   AY069743 AAL39888   BF487214   BF487864   BF488203   BF489939   BF490998   BF491883   BF496185   BF497514   BF497689   BF497985   BF498062   BF498506   BF498674   BF499697   BF500539   BF500545   BF500554   BF500682   BF501324   BF501720   BF502195   BF502818   BF503623   BF504343   BF506613   BG633748   BG634168   BG634596   BG635257   BI216928   BI216982   BI364706   BT001581 AAN71336   BT126033   CA803794   CA805181   CK658155   CO264891   CO324293   EC063303   EC084453   EC221332   GH739566   GH740591   GH744131   GH746355   GH746361   GH746837   GH762257   GH762943   GH764707   GH765573   GH767514   GH779491   U51045 AAB01144
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q8SZW5 Q24196
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   (Campbell et al., 2003) first instar larval stage -- second instar larval stage   organism   (Campbell et al., 2003) third instar larval stage   organism | intense   (Campbell et al., 2003) pharate adult stage P8 -- P15   organism   (Campbell et al., 2003) day 1 of adulthood   organism   (Campbell et al., 2003) adult head | intense   (Campbell et al., 2003) day 5 of adulthood   organism | intense   (Campbell et al., 2003)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: larval midgut, larval/adult Malpighian tubules, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, adult midgut, larval/adult hindgut, adult heart, larval/adult fat body, adult salivary gland, adult spermathecae, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut    541.5 Larval Hindgut    266.6 Larval Malpighian Tubules    642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules    1174.1 Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis    791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut  (541.5) Larval Hindgut    266.6 Larval Malpighian Tubules  (642.5) Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules  (1174.1) Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis  (791) Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut    541.5 Larval Hindgut    266.6 Larval Malpighian Tubules    642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules  (1174.1) Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis    791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut    541.5 Larval Hindgut    266.6 Larval Malpighian Tubules    642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules    1174.1 Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis    791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut    541.5 Larval Hindgut    266.6 Larval Malpighian Tubules    642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules    1174.1 Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis    791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut  541.5 Larval Hindgut    266.6 Larval Malpighian Tubules  642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules  (1174.1) Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis  791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut    541.5 Larval Hindgut    266.6 Larval Malpighian Tubules    642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules  1174.1 Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis    791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    22.25 Larval Midgut    541.5 Larval Hindgut    266.6 Larval Malpighian Tubules    642.5 Larval Fat Body    171.8 Larval Salivary Gland    92.1 Larval Trachea    87.5 Larval Carcass    159.6 Adult Head    71.9 Adult Eye    64.725 Adult Brain    25.9 Adult Thoracic-Abdominal Ganglion    42.1 Adult Crop    225.5 Adult Midgut    259.2 Adult Hindgut    300.9 Adult Malpighian Tubules    1174.1 Adult Fat Body    280.6 Adult Salivary Gland    406.9 Adult Heart    328.6 Adult VirginFemale Spermatheca    132.3 Adult InseminatedFemale Spermatheca    112.1 Adult Ovary    76.2 Adult Testis    791 Adult Male Accessory Gland    109 Adult Carcass    250.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    71.9   NA  Eye    64.725   NA  Brain    25.9   22.25  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    42.1   NA  Crop    225.5   541.5  Midgut    259.2   266.6  Hindgut    300.9   642.5  Malpighian Tubules    1174.1   171.8  Fat Body    280.6   92.1  Salivary Gland    406.9   NA  Heart    328.6   87.5  Trachea    NA   NA  VirginFemale Spermatheca    132.3   NA  InseminatedFemale Spermatheca    112.1   NA  Ovary    76.2   NA  Testis    791   NA  Male Accessory Gland    109   159.6  Carcass    250.2 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    42 digestive system, 4-day adult    22 digestive system, 20-day adult    31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male    165 accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (34) central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering  (39) digestive system, 1-day adult  (42) digestive system, 4-day adult    22 digestive system, 20-day adult  (31) fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8  26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male  (165) accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    42 digestive system, 4-day adult    22 digestive system, 20-day adult    31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male  (165) accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    42 digestive system, 4-day adult    22 digestive system, 20-day adult    31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male    165 accessory gland, mated 4-day male    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    42 digestive system, 4-day adult    22 digestive system, 20-day adult    31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male    165 accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (34) central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering  (39) digestive system, 1-day adult  (42) digestive system, 4-day adult    22 digestive system, 20-day adult  31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8  26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male  (165) accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    42 digestive system, 4-day adult    22 digestive system, 20-day adult    31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male  (165) accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    34 central nervous system, larvae L3    2 central nervous system, pupae P8    1 head, virgin 1-day female    5 head, virgin 4-day female    4 head, virgin 20-day female    6 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    6 head, mated 1-day male    7 head, mated 4-day male    4 head, mated 20-day male    8 salivary gland, larvae L3 wandering    6 salivary gland, white prepupae    23 digestive system, larvae L3 wandering    39 digestive system, 1-day adult    42 digestive system, 4-day adult    22 digestive system, 20-day adult    31 fat body, larvae L3 wandering    3 fat body, white prepupae    15 fat body, pupae P8    26 carcass, larvae L3 wandering    8 carcass, 1-day adult    11 carcass, 4-day adult    9 carcass, 20-day adult    12 ovary, virgin 4-day female    9 ovary, mated 4-day female    7 testis, mated 4-day male    165 accessory gland, mated 4-day male    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr    30 embryo 22-24hr    42 larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr    37 white prepupae 24hr    28 pupae 2d postWPP    31 pupae 3d postWPP    34 pupae 4d postWPP    35 adult male 01day    66 adult male 05day    80 adult male 30day    72 adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr  (30) embryo 22-24hr  (42) larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2  (31) larva L3 puffstage 3-6  (29) larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr  (37) white prepupae 24hr  (28) pupae 2d postWPP  (31) pupae 3d postWPP  (34) pupae 4d postWPP  (35) adult male 01day  (66) adult male 05day  (80) adult male 30day  (72) adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr    30 embryo 22-24hr    42 larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr    37 white prepupae 24hr    28 pupae 2d postWPP    31 pupae 3d postWPP    34 pupae 4d postWPP    35 adult male 01day    66 adult male 05day    80 adult male 30day    72 adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr    30 embryo 22-24hr    42 larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr    37 white prepupae 24hr    28 pupae 2d postWPP    31 pupae 3d postWPP    34 pupae 4d postWPP    35 adult male 01day    66 adult male 05day    80 adult male 30day    72 adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr    30 embryo 22-24hr    42 larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr    37 white prepupae 24hr    28 pupae 2d postWPP    31 pupae 3d postWPP    34 pupae 4d postWPP    35 adult male 01day    66 adult male 05day    80 adult male 30day    72 adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr  30 embryo 22-24hr  (42) larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2  31 larva L3 puffstage 3-6  29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr  (37) white prepupae 24hr  28 pupae 2d postWPP  31 pupae 3d postWPP  (34) pupae 4d postWPP  (35) adult male 01day  (66) adult male 05day  (80) adult male 30day  (72) adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr    30 embryo 22-24hr    42 larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr    37 white prepupae 24hr    28 pupae 2d postWPP    31 pupae 3d postWPP    34 pupae 4d postWPP    35 adult male 01day    66 adult male 05day    80 adult male 30day    72 adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    12 embryo 02-04hr    2 embryo 04-06hr    1 embryo 06-08hr    0 embryo 08-10hr    1 embryo 10-12hr    2 embryo 12-14hr    4 embryo 14-16hr    7 embryo 16-18hr    10 embryo 18-20hr    17 embryo 20-22hr    30 embryo 22-24hr    42 larva L1    25 larva L2    18 larva L3 12hr old    21 larva L3 puffstage 1-2    31 larva L3 puffstage 3-6    29 larva L3 puffstage 7-9    25 white prepupae new    23 white prepupae 12hr    37 white prepupae 24hr    28 pupae 2d postWPP    31 pupae 3d postWPP    34 pupae 4d postWPP    35 adult male 01day    66 adult male 05day    80 adult male 30day    72 adult female 01day    19 adult female 05day    19 adult female 30day    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2    40 embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2  (40) embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2    40 embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2    40 embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2    40 embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2  (40) embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2    40 embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    9 Schneider line 2 Sg4    3 embryonic 1182-4H    6 embryonic GM2    40 embryonic Kc167    2 embryonic S1    12 embryonic S3    0 leg disc CME L1    3 wing disc CME-W2    2 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    3 wing disc ML-DmD21    10 wing disc ML-DmD32    1 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    12 antennal disc ML-DmD20-c5    3 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    9 CNS ML-DmBG2-c2    6 tumorous blood cells mbn2    3 ovary fGS/OSS    5 ovary OSC    2 ovary OSS    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    37 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    37 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    32 Rotenone 2 μg 12 hrs, larvae L3    29 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3  (27) Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3  (37) Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (37) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult  (32) Rotenone 2 μg 12 hrs, larvae L3  (29) Rotenone 8 μg 12 hrs, larvae L3  (27) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    37 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    37 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    32 Rotenone 2 μg 12 hrs, larvae L3    29 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    37 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    37 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    32 Rotenone 2 μg 12 hrs, larvae L3    29 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    37 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    37 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    32 Rotenone 2 μg 12 hrs, larvae L3    29 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3  27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  26 Ethanol 5% 3 hrs, larvae L3  (37) Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3  (37) Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult  32 Rotenone 2 μg 12 hrs, larvae L3  29 Rotenone 8 μg 12 hrs, larvae L3  27 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    37 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    37 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    32 Rotenone 2 μg 12 hrs, larvae L3    29 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    8 cold shock, 4-day adult    6 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    27 Cadmium 50 mM 12 hrs, larvae L3    20 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    20 Copper 0.5 mM 12 hrs, larvae L3    9 Copper 15 mM 48 hrs, 4-day adult    11 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    26 Ethanol 5% 3 hrs, larvae L3    37 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    37 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    15 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    32 Rotenone 2 μg 12 hrs, larvae L3    29 Rotenone 8 μg 12 hrs, larvae L3    27 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_10 mE1_20_mRNA_expression_cluster_15
External Data & Images
Linkouts	BDGP expression data • LP07235 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015571 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1257-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable α-Est3c05084 viable, with Scer\GAL4pnr-MD237 α-Est3GD7760 Sterility Allele fertile α-Est3c05084 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of α-Est3 Class Mutagen Stocks Known lesion α-Est3c05084 piggyBac transposase 2 -- α-Est3MI02439 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of α-Est3 Class Mutagen Stocks Known lesion α-Est3GD7760 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes α-Est3KK105387 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes α-Est3NIG.1257R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}α-Est3MI02439 NA PBac{PB}α-Est3c05084 NA
Gene Ontology: Function, Process & Cellular Component ( 1 unique term )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 1 term )
Molecular Function
CV term Evidence References
carboxylesterase activity inferred from biological aspect of ancestor with PANTHER:PTN000168392 (assigned by RefGenome) (Gaudet et al., 2010) inferred from sequence or structural similarity with UniProtKB:P16854 (FlyBase, 1992-) non-traceable author statement (Robin, 1996.3.11)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
α-Est3 - CG14767 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) α-Est3 - Cg25C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions α-Est3 allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015571 DroID - A comprehensive database of gene and protein interactions. • FBgn0015571
Orthologs
OrthoDB Orthologs (28) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62Z58X Dipteran inclusive ortholog search EOG6NP7BP Insect inclusive ortholog search EOG6S7HK4 Arthropod inclusive ortholog search EOG612JMJ Metazoa inclusive ortholog search EOG683RXJ
Orthologs in Drosophila Species (EOG62Z58X)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\α-Est3     Drosophila simulans   Dsim\GD19470 Y   Drosophila sechellia   Dsec\GM10468 Y   Drosophila erecta   Dere\GG25072 Y   Drosophila yakuba   Dyak\GE25780 Y   Drosophila ananassae   Dana\GF17292 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA26213     Drosophila pseudoobscura pseudoobscura   Dpse\GA11698 Y   Drosophila persimilis   Dper\GL12165 Y   Drosophila persimilis   Dper\GL12164     Drosophila willistoni   Dwil\GK11068 Y   Drosophila virilis   Dvir\GJ10228 Y   Drosophila mojavensis   Dmoj\GI23472 Y   Drosophila grimshawi   Dgri\GH19265 Y
Orthologs in non-Drosophila Dipterans (EOG6NP7BP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL017071 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010389 Y Aedes aegypti Yellow fever mosquito  Aaeg\AAEL015578 Y Anopheles gambiae Malaria mosquito  Agam\AGAP006228 Y Anopheles gambiae Malaria mosquito  Agam\AGAP006227 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013918 Y Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013917 Y
Orthologs in non-Dipteran Insects (EOG6S7HK4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Atta cephalotes Leafcutter ant  Acep\ACEP18171 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17276 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17284 Y Bombyx mori Silkmoth  Bmor\BGIBMGA010988 Y Bombyx mori Silkmoth  Bmor\BGIBMGA004683 Y
Orthologs in non-Insect Arthropods (EOG612JMJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_312876 Y
Orthologs in non-Arthropod Metazoa (EOG683RXJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Rattus norvegicus Norway rat  Rnor\Ces2j Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD19470 Drosophila sechellia Dsec\GM10468 Drosophila erecta Dere\GG25072 Drosophila yakuba Dyak\GE25780 Drosophila ananassae Dana\GF17292 Drosophila pseudoobscura pseudoobscura Dpse\GA11698 Drosophila persimilis Dper\GL12165 Drosophila willistoni Dwil\GK11068 Drosophila virilis Dvir\GJ10228 Drosophila mojavensis Dmoj\GI23472 Drosophila grimshawi Dgri\GH19265
Stocks & Reagents
Stocks Listed in FlyBase ( 6 )
Bloomington	17691 w1118; PBac{PB}α-Est3c05084 35982 y1 w*; Mi{MIC}α-Est3MI02439
Harvard	- PBac{PB}α-Est3c05084
VDRC	v101671 P{KK105387}VIE-260B v31972 w1118; P{GD7760}v31972 v31974 w1118; P{GD7760}v31974/CyO
Genomic Clones ( 2 )
	BACR06G09 BACR37K05 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 53 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT03093 LP07235 RE24420
Other clones	DPiM_LP07235
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT04709 AT05687 AT09141 AT09920 AT11696 AT11923 AT12330 AT12421 AT12927 AT13126 AT14329 AT15519 AT15527 AT15537 AT15678 AT16613 AT17218 AT17851 AT18648 AT21939 AT22951 AT23414 AT26178 AT27646 AT28647 AT29469 AT30283 AT30854 AT31777 GH22655 LP14781 RE24487 RE49874
Other clones	EK140836 EK193142 ESG0110c.E21_D12 ESG0116d.B21_E12 RP001000284 RP001000527 RP001005024 RP001005375 RP001017673 RP001019483 RP001029176 RP001035812 RP001036413 RP001040846 RP001047030 RP001051708
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015571 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40907
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: α-Est3 CG1257 (Wang, 1999.11)
Source for database merge of
Additional comments
Other Comments
	Eleven putative esterase genes from the region containing the α-esterase cluster are cloned. (Russell et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079543 NM_001170069 NM_001275415 RefSeq (Proteins) • NP_524267 NP_001163540 NP_001262344 DDBJ / EMBL / GenBank • AAB01144 AAF54004 AAL39888 AAN71336 ACZ94837 AGB95727 AI293962 AW944073 AY069743 BI216928 BT001581 U51045 Entrez Gene - A searchable database of RefSeq genes. • 40907 UniProtKB/TrEMBL • Q8SZW5 Q24196
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Carboxylesterase, type B (IPR002018) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LP07235
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015571 DroID - A comprehensive database of gene and protein interactions. • FBgn0015571 DRSC - Results from RNAi screens. • FBgn0015571 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015571 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG1257-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015571 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 40907 modMine - Data generated by the modENCODE project. • FBgn0015571
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	aE3 (Robin, 1996.3.11) alpha-Est3 (Leemans et al., 2000, Izutsu et al., 2012, Japanese National Institute of Genetics, 2012.5.21) B (Robin et al., 1996) CG1257 (Keleman et al., 2009.8.5) DmαE3 (de Q. Robin et al., 2000, Campbell et al., 2003) unnamed (Russell et al., 1996) αE3 (de Q. Robin et al., 2000, Robin et al., 1996, de Q. Robin et al., 2000, Campbell et al., 2003) α-Est3 (Casillas et al., 2006) α-Ests (Ranz et al., 2001, Ranz et al., 2001)
Name Synonym	fragment B (Robin et al., 1996) α-Esterase-3
Secondary FlyBase IDs
References ( 26 )
Generate a list of	All references relating to this gene ( 26 )
List References by type	Research paper  ( 10 ) Supplementary material  ( 4 ) Personal communication to FlyBase  ( 6 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Website  ( 1 )
Recent research papers ( 1 )
	Izutsu et al., 2012, PLoS ONE 7(3): e33288 Genome features of "dark-fly", a Drosophila line reared long-term in a dark environment. [FBrf0217825] Show all research papers ...