General Information
Symbol
Dmel\Apc
Species
D. melanogaster
Name
APC-like
Annotation Symbol
CG1451
Feature Type
FlyBase ID
FBgn0015589
Gene Model Status
Stock Availability
Gene Snapshot
APC-like is one of two Drosophila APC family proteins. It is a key negative regulator of Wingless signaling, as a critical component of the destruction complex that phosphorylates beta-catenin and thus targets it for ubiquitination and proteasomal destruction. It is the primary family member during CNS and eye development, and functions redundantly with Apc2 in adult development. [Date last reviewed: 2016-08-25]
Also Known As
APC1, D-APC, dAPC, dAPC1
Genomic Location
Cytogenetic map
Sequence location
3R:28,832,301..28,844,748 [-]
Recombination map
3-97
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
GO Summary Ribbons
Families, Domains and Molecular Function
Protein Family (UniProt, Sequence Similarities)
-
Molecular Function (see GO section for details)
Experimental Evidence
Predictions / Assertions
Summaries
Gene Group Membership
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of canonical Wnt signaling down-regulation the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
BETA CATENIN DESTRUCTION COMPLEX -
The β-catenin destruction complex is a cytoplasmic protein complex that phosphorylates β-catenin, targeting it for ubiquitin-mediated proteolysis. In the absence of Wnt signaling, the β-catenin destruction complex constitutively suppresses the canonical Wnt signaling pathway by preventing the accumulation of cytoplasmic β-catenin (arm). (Adapted from FBrf0217546).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Apc or the JBrowse view of Dmel\Apc for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.48
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
Translational frameshifting postulated (FBrf0216436).
gene_with_transcript_with_translational_frameshift ; SO:0000712
Gene model reviewed during 6.07
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0085351
8441
2417
FBtr0336767
8317
2417
FBtr0445314
8441
1597
Additional Transcript Data and Comments
Reported size (kB)
8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0084720
261.3
2417
5.35
FBpp0307743
261.3
2417
5.35
FBpp0401491
174.6
1597
5.79
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2417 aa isoforms: Apc-PA, Apc-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
2416 (aa); 260 (kD predicted)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Apc using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (26 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with UniProtKB:Q9VH90
(assigned by UniProt )
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
Biological Process (17 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from genetic interaction with FLYBASE:Apc2; FB:FBgn0026598
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
Cellular Component (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
colocalizes_with cytoplasmic microtubule
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
Expression Data
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Apc transcripts are observed in very early embyros and are not seen again until after germ band retraction. At that time they are concentrated in the CNS where they are restricted to postmitotic cells. Transcripts are also found in the epidermis.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: Expression more prominent in CNS.

Additional Descriptive Data
The Apc protein colocalizes with shot on microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site.
Embryonic expression of Apc protein is ubiquitous, but more pronounced in the CNS.
Apc protein is detected in the nervous system of embryos from germ-band retraction on. It localizes to axon fiber tracts and motor neurons. Initially, similar levels are found in longitudinal and commissural tracts. Later, staining is more intense in the longitudinal tracts and is distributed in patches or streaks within specific fibers in the longitudinal tracts.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Apc in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Allele of Apc
Class
Mutagen
Associated Insertion
Stocks
Known lesion
    Yes
      0
      Yes
        0
        Yes
        Other relevant insertions
        Transgenic Constructs ( 11 )
        Deletions and Duplications ( 5 )
        Summary of Phenotypes
        For more details about a specific phenotype click on the relevant allele symbol.
        Lethality
        Allele
        Other Phenotypes
        Allele
        Phenotype manifest in
        Allele
        male germline stem cell & centrosome (with Df(3R)3450)
        male germline stem cell & spindle (with Df(3R)3450)
        neuron & retina & pupa
        Orthologs
        Human Orthologs (via DIOPT v7.1)
        Homo sapiens (Human) (2)
        Species\Gene Symbol
        Score
        Best Score
        Best Reverse Score
        Alignment
        Complementation?
        Transgene?
        9 of 15
        Yes
        Yes
         
        8 of 15
        No
        Yes
        Model Organism Orthologs (via DIOPT v7.1)
        Mus musculus (laboratory mouse) (2)
        Species\Gene Symbol
        Score
        Best Score
        Best Reverse Score
        Alignment
        Complementation?
        Transgene?
        8 of 15
        Yes
        Yes
        8 of 15
        Yes
        Yes
        Rattus norvegicus (Norway rat) (2)
        8 of 13
        Yes
        Yes
        8 of 13
        Yes
        Yes
        Xenopus tropicalis (Western clawed frog) (2)
        4 of 12
        Yes
        Yes
        4 of 12
        Yes
        Yes
        Danio rerio (Zebrafish) (2)
        6 of 15
        Yes
        Yes
        2 of 15
        No
        No
        Caenorhabditis elegans (Nematode, roundworm) (1)
        5 of 15
        Yes
        No
        Arabidopsis thaliana (thale-cress) (0)
        No orthologs reported.
        Saccharomyces cerevisiae (Brewer's yeast) (0)
        No orthologs reported.
        Schizosaccharomyces pombe (Fission yeast) (0)
        No orthologs reported.
        Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900FA )
        Organism
        Common Name
        Gene
        AAA Syntenic Ortholog
        Multiple Dmel Genes in this Orthologous Group
        Drosophila melanogaster
        fruit fly
        Drosophila suzukii
        Spotted wing Drosophila
        Drosophila simulans
        Drosophila sechellia
        Drosophila erecta
        Drosophila yakuba
        Drosophila ananassae
        Drosophila pseudoobscura pseudoobscura
        Drosophila persimilis
        Drosophila willistoni
        Drosophila virilis
        Drosophila mojavensis
        Drosophila grimshawi
        Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500B1 )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Musca domestica
        House fly
        Glossina morsitans
        Tsetse fly
        Lucilia cuprina
        Australian sheep blowfly
        Mayetiola destructor
        Hessian fly
        Anopheles gambiae
        Malaria mosquito
        Culex quinquefasciatus
        Southern house mosquito
        Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W008P )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Bombyx mori
        Silkmoth
        Danaus plexippus
        Monarch butterfly
        Heliconius melpomene
        Postman butterfly
        Apis florea
        Little honeybee
        Apis mellifera
        Western honey bee
        Bombus impatiens
        Common eastern bumble bee
        Bombus terrestris
        Buff-tailed bumblebee
        Linepithema humile
        Argentine ant
        Megachile rotundata
        Alfalfa leafcutting bee
        Nasonia vitripennis
        Parasitic wasp
        Dendroctonus ponderosae
        Mountain pine beetle
        Dendroctonus ponderosae
        Mountain pine beetle
        Tribolium castaneum
        Red flour beetle
        Pediculus humanus
        Human body louse
        Rhodnius prolixus
        Kissing bug
        Cimex lectularius
        Bed bug
        Acyrthosiphon pisum
        Pea aphid
        Zootermopsis nevadensis
        Nevada dampwood termite
        Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0086 )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Strigamia maritima
        European centipede
        Ixodes scapularis
        Black-legged tick
        Stegodyphus mimosarum
        African social velvet spider
        Stegodyphus mimosarum
        African social velvet spider
        Stegodyphus mimosarum
        African social velvet spider
        Stegodyphus mimosarum
        African social velvet spider
        Stegodyphus mimosarum
        African social velvet spider
        Tetranychus urticae
        Two-spotted spider mite
        Tetranychus urticae
        Two-spotted spider mite
        Daphnia pulex
        Water flea
        Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00D4 )
        Organism
        Common Name
        Gene
        Multiple Dmel Genes in this Orthologous Group
        Strongylocentrotus purpuratus
        Purple sea urchin
        Strongylocentrotus purpuratus
        Purple sea urchin
        Ciona intestinalis
        Vase tunicate
        Ciona intestinalis
        Vase tunicate
        Gallus gallus
        Domestic chicken
        Gallus gallus
        Domestic chicken
        Human Disease Model Data
        FlyBase Human Disease Model Reports
        Alleles Reported to Model Human Disease (Disease Ontology)
        Download
        Models ( 2 )
        Interactions ( 1 )
        Allele
        Disease
        Interaction
        References
        is ameliorated by ewgP1
        Comments ( 0 )
         
        Human Orthologs (via DIOPT v7.1)
        Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser

        Please look at the Interaction Group reports for full details of the physical interactions
        protein-protein
        Interacting group
        Assay
        References
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement

        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Gene Group - Pathway Membership (FlyBase)
        Negative Regulators of Wnt-TCF Signaling Pathway -
        Negative regulators of canonical Wnt signaling down-regulation the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        SignaLink - A signaling pathway resource with multi-layered regulatory networks.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-97
        Cytogenetic map
        Sequence location
        3R:28,832,301..28,844,748 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        98E5-98E6
        Limits computationally determined from genome sequence between P{PZ}l(3)0648706487 and P{EP}EP3390EP3390
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        98F-98F
        (determined by in situ hybridisation)
        98D-98E
        (determined by in situ hybridisation)
        Experimentally Determined Recombination Data
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (11)
        Genomic Clones (21)
        cDNA Clones (28)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequences
        BDGP DGC clones
          Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
              Other clones
                RNAi and Array Information
                Linkouts
                DRSC - Results frm RNAi screens
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                Antibody Information
                Laboratory Generated Antibodies
                Commercially Available Antibodies
                 
                Other Information
                Relationship to Other Genes
                Source for database identify of
                Source for database merge of
                Source for merge of: Apc CG1451
                Additional comments
                "S(CycEJP)1S3" is unlikely to correspond to "Apc"; mutations in the two loci complement each other.
                Other Comments
                Apc and Apc2 are required in intestinal stem cells in the adult midgut to regulate proliferation.
                dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
                shot recruits Eb1/Apc and promotes microtubule assembly at the muscle-tendon junction.
                Regulation of arm by Apc inhibits neuronal apoptosis during retinal development.
                Apc is capable of down-regulating β-catenin in a colon carcinoma cell line and of interacting with the arm protein in vivo. Its expression pattern and preliminary genetic analysis suggest that it may not be an essential component of arm regulation in the wg pathway.
                Identification: cDNA expression library with an antibody against Hsap\APC.
                Origin and Etymology
                Discoverer
                Etymology
                Identification
                External Crossreferences and Linkouts ( 51 )
                Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMine - An integrated database for Drosophila genomics
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                KEGG Genes - Molecular building blocks of life in the genomic space.
                modMine - A data warehouse for the modENCODE project
                SignaLink - A signaling pathway resource with multi-layered regulatory networks.
                Synonyms and Secondary IDs (24)
                Reported As
                Symbol Synonym
                Secondary FlyBase IDs
                • FBgn0039604
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                References (265)