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General Information
Symbol
Dmel\Apc
Species
D. melanogaster
Name
APC-like
Annotation Symbol
CG1451
Feature Type
FlyBase ID
FBgn0015589
Gene Model Status
Stock Availability
Gene Snapshot
APC-like (Apc) encodes one of two Drosophila APC family proteins. It is a key negative regulator of Wingless signaling, as a critical component of the destruction complex that phosphorylates beta-catenin and thus targets it for ubiquitination and proteasomal destruction. It is the primary family member during CNS and eye development, and functions redundantly with the product of Apc2 in adult development. [Date last reviewed: 2019-03-07]
Also Known As
APC1, D-APC, dAPC, adenomatous polyposis coli, dAPC1
Key Links
Genomic Location
Cytogenetic map
Sequence location
3R:28,832,301..28,844,748 [-]
Recombination map
3-97
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
Predictions / Assertions
Summaries
Pathway (FlyBase)
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of canonical Wnt signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
Gene Group (FlyBase)
BETA CATENIN DESTRUCTION COMPLEX -
The β-catenin destruction complex is a cytoplasmic protein complex that phosphorylates β-catenin, targeting it for ubiquitin-mediated proteolysis. In the absence of Wnt signaling, the β-catenin destruction complex constitutively suppresses the canonical Wnt signaling pathway by preventing the accumulation of cytoplasmic β-catenin (arm). (Adapted from FBrf0217546).
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Apc or the JBrowse view of Dmel\Apc for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.48
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.55
Translational frameshifting postulated (FBrf0216436).
gene_with_transcript_with_translational_frameshift ; SO:0000712
Gene model reviewed during 6.07
Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0085351
8441
2417
FBtr0336767
8317
2417
FBtr0445314
8441
1597
Additional Transcript Data and Comments
Reported size (kB)
8 (northern blot)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0084720
261.3
2417
5.35
FBpp0307743
261.3
2417
5.35
FBpp0401491
174.6
1597
5.79
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2417 aa isoforms: Apc-PA, Apc-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
2416 (aa); 260 (kD predicted)
Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Apc using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (26 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from physical interaction with UniProtKB:Q9VH90
(assigned by UniProt )
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
Biological Process (17 terms)
Terms Based on Experimental Evidence (11 terms)
CV Term
Evidence
References
inferred from genetic interaction with FLYBASE:Apc2; FB:FBgn0026598
Terms Based on Predictions or Assertions (7 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
Cellular Component (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
colocalizes_with cytoplasmic microtubule
inferred from biological aspect of ancestor with PANTHER:PTN000288612
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Apc transcripts are observed in very early embyros and are not seen again until after germ band retraction. At that time they are concentrated in the CNS where they are restricted to postmitotic cells. Transcripts are also found in the epidermis.
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
organism | ubiquitous

Comment: Expression more prominent in CNS.

Additional Descriptive Data
The Apc protein colocalizes with shot on microtubules in mature tendon cells of third instar larvae. There is a focus of staining overlapping a compact microtubule array near the muscle-tendon junction. This unique subcellular structure extends to the cuticle attachment site.
Embryonic expression of Apc protein is ubiquitous, but more pronounced in the CNS.
Apc protein is detected in the nervous system of embryos from germ-band retraction on. It localizes to axon fiber tracts and motor neurons. Initially, similar levels are found in longitudinal and commissural tracts. Later, staining is more intense in the longitudinal tracts and is distributed in patches or streaks within specific fibers in the longitudinal tracts.
Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Apc in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 8 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Apc
Transgenic constructs containing regulatory region of Apc
Deletions and Duplications ( 5 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
male germline stem cell & centrosome (with Df(3R)3450)
male germline stem cell & spindle (with Df(3R)3450)
neuron & retina & pupa
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
9 of 15
Yes
Yes
 
8 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
8 of 15
Yes
Yes
8 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (2)
8 of 13
Yes
Yes
8 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
4 of 12
Yes
Yes
4 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
6 of 15
Yes
Yes
2 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
5 of 15
Yes
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900FA )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500B1 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W008P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0086 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00D4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (1)
4 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Potential Models Based on Orthology ( 4 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
is ameliorated by ewgP1
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Gene Group - Pathway Membership (FlyBase)
Negative Regulators of Wnt-TCF Signaling Pathway -
Negative regulators of canonical Wnt signaling down-regulate the pathway, resulting in the attenuation of transcriptional regulation mediated by β-catenin (arm).
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
3R
Recombination map
3-97
Cytogenetic map
Sequence location
3R:28,832,301..28,844,748 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
98E5-98E6
Limits computationally determined from genome sequence between P{PZ}l(3)0648706487 and P{EP}EP3390EP3390
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
98F-98F
(determined by in situ hybridisation)
98D-98E
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (11)
Genomic Clones (21)
cDNA Clones (28)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences
BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
        Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of
          Source for database merge of
          Source for merge of: Apc CG1451
          Additional comments
          "S(CycEJP)1S3" is unlikely to correspond to "Apc"; mutations in the two loci complement each other.
          Other Comments
          Apc and Apc2 are required in intestinal stem cells in the adult midgut to regulate proliferation.
          dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
          shot recruits Eb1/Apc and promotes microtubule assembly at the muscle-tendon junction.
          Regulation of arm by Apc inhibits neuronal apoptosis during retinal development.
          Apc is capable of down-regulating β-catenin in a colon carcinoma cell line and of interacting with the arm protein in vivo. Its expression pattern and preliminary genetic analysis suggest that it may not be an essential component of arm regulation in the wg pathway.
          Identification: cDNA expression library with an antibody against Hsap\APC.
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 60 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
          Other crossreferences
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          KEGG Genes - Molecular building blocks of life in the genomic space.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          modMine - A data warehouse for the modENCODE project
          SignaLink - A signaling pathway resource with multi-layered regulatory networks.
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyMine - An integrated database for Drosophila genomics
          Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (genetic) - An integrated Molecular Interaction Database
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Synonyms and Secondary IDs (24)
          Reported As
          Symbol Synonym
          Secondary FlyBase IDs
          • FBgn0039604
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (276)