N-cadherin, DN-cadherin, Ncad, N-Cad, DN-Cad
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
None of the polypeptides share 100% sequence identity.
3097 (aa); 300 (kD observed)
Three calcium ions are usually bound at the interface of each cadherin domain and rigidify the connections, imparting a strong curvature to the full-length ectodomain.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CadN using the Feature Mapper tool.
CadN transcripts are first seen in nuclei of presumptive mesodermal cells prior to stage 5. Transport to the cytoplasm begins at around stage 6-7. By stage 8, transcripts are distributed throughout the nucleus.
Axons in the core layer of the larval mushroom body of first and second instar larvae intensely expressed CadN, while expression in surrounding mature axons was much weaker.
Strong CadN immunoreactivity was detected in the cell bodies of developing MB neurons, as well as in the core axons of peduncles and lobes. In contrast, CadN expression was dramatically downregulated in the cell bodies of mature neurons, and in axons located at the periphery of the peduncles and lobes. In the adults, all mature MB neurons exhibited reduced expression of CadN.
At 25 hr APF, CadN is strongly expressed between the R7 and R8 growth cones that mark the boundaries of the outer medulla at this stage. By 48 hr APF, the region has divided into two CadN-rich regions divided by a zone of weaker staining. At 75 hr APF prominent CadN expression is seen in a single layer in the distal part of the outer medulla. Strong CadN expression is seen in M2 in later pual stages. CadN is strongly expressed on growth cones of lamina neurons during development.
detected in the future pretarsus region. These pretarsus signals became stronger during early third instar. CadN expression in tarsal segment 5 could be discerned in the region surrounding the central pretarsus expression domain from mid-third instar onwards.
CadN is localized to the adherens junctions of the cell-cell interfaces of cone cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\CadN in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Alternative exon 18A encodes for isoforms that are required for R1-R7 targeting.
CadN plays a crucial role in protoglomerulus formation but is largely dispensible for targeting olfactory receptor neurons to the appropriate region of the antennal lobe.
CadN does not have an instructive role in selecting dendritic targets in the projection neurons, but rather is essential for projection neurons to restrict their dendrites to single glomeruli. CadN appears to mediate dendro-dendritic interactions between projection neurons. CadN is also essential for the development of projection neuron axon terminal arbors at two distinct targets; regulating branch stability in the lateral horn and restricting high-order branching in the mushroom body.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Mutants exhibit various defects in axon fascicle formation.
Mutant analysis suggests that processes of axon patterning critically depend on CadN-mediated axon-axon interactions.
Isolated from a subtractive cDNA library enriched in sequences expressed in the mesoderm.