FB2019_05, released Oct 15, 2019

Gene: Dmel\Cdk8

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General Information
Symbol
Dmel\Cdk8
Species
D. melanogaster
Name
Cyclin-dependent kinase 8
Annotation Symbol
CG10572
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015618
Gene Model Status
Current
Stock Availability
10 publicly available
Enzyme Name (EC)
Cyclin-dependent kinase (2.7.11.22)
[RNA-polymerase]-subunit kinase (2.7.11.23)
Gene Snapshot
In progress.Contributions welcome.
Other Summaries
Key Links
Genomic Location
Cytogenetic map
67C10-67C10
Sequence location
3L:9,837,080..9,838,670 [-]
Recombination map
3-31
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. (Q9VT57)
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
kinase activity; protein binding
Predictions / Assertions
ATP binding; cyclin-dependent protein serine/threonine kinase activity; RNA polymerase II CTD heptapeptide repeat kinase activity
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + a protein = ADP + a phosphoprotein (2.7.11.22)
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate (2.7.11.23)
Summaries
Gene Group (FlyBase)
CYCLIN DEPENDENT KINASES -
Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose activity depends on a regulatory cyclin subunit. (Adapted from PMID:25180339).
MEDIATOR COMPLEX -
The Mediator (MED) complex serves as a hub for transcriptional signaling events. MED bridges transcriptional activators and repressors. This large multi-protein complex has a central core conserved from yeast to humans. The core is composed of three modules: head, middle, and tail. A separate module, the Cdk8 kinase module, composed of Cdk8, CycC, kto and skd, serves as a regulator. (Adapted from FBrf0226415 and FBrf0191850).
Protein Function (UniProtKB)
Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation.
(UniProt, Q9VT57)
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Cdk8 or the JBrowse view of Dmel\Cdk8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.44
Gene model reviewed during 5.45
Gene model reviewed during 5.55
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Cdk8-RA
FBtr0076369
NM_080487
1529
454
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Cdk8-PA
FBpp0076098
53.7
454
8.87
NP_536735
AAF50197
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)
Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.
(UniProt, Q9VT57)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdk8 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (15 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
kinase activity
inferred from direct assay
(Tang et al., 2018)
protein binding
inferred from physical interaction with UniProtKB:Q8MSX2
(assigned by UniProt )
(Park et al., 2001)
inferred from physical interaction with UniProtKB:Q7KTX8
(assigned by UniProt )
(Loncle et al., 2007)
inferred from physical interaction with UniProtKB:Q9VW47
(assigned by UniProt )
(Loncle et al., 2007)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
ATP binding
inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
cyclin-dependent protein serine/threonine kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000623091
(assigned by GO_Central )
(Gaudet et al., 2011)
RNA polymerase II CTD heptapeptide repeat kinase activity
inferred from biological aspect of ancestor with PANTHER:PTN000623092
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (8 terms)
Terms Based on Experimental Evidence (8 terms)
CV Term
Evidence
References
axon guidance
inferred from mutant phenotype
(Berger et al., 2008)
chaeta development
inferred from mutant phenotype
(Loncle et al., 2007)
G1/S transition of mitotic cell cycle
inferred from mutant phenotype
(assigned by UniProt )
(Leclerc et al., 1996)
imaginal disc-derived leg segmentation
inferred from mutant phenotype
(Loncle et al., 2007)
positive regulation of autophagy
inferred from mutant phenotype
(Tang et al., 2018)
protein phosphorylation
inferred from mutant phenotype
(Sopko et al., 2014)
inferred from direct assay
(assigned by UniProt )
(Leclerc et al., 1996)
inferred from direct assay
(Tang et al., 2018)
sex comb development
inferred from mutant phenotype
(Loncle et al., 2007)
snRNA 3'-end processing
inferred from direct assay
(Chen et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
protein phosphorylation
inferred from biological aspect of ancestor with PANTHER:PTN000623091
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
mediator complex
inferred from direct assay
(Park et al., 2003)
(Loncle et al., 2007)
nucleus
inferred from direct assay
(Park et al., 2003)
inferred from direct assay
(assigned by UniProt )
(Leclerc et al., 1996)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
mediator complex
inferred from sequence or structural similarity with UniProtKB:P49336
(Boube et al., 2000)
inferred from biological aspect of ancestor with PANTHER:PTN000623092
(assigned by GO_Central )
(Gaudet et al., 2011)
nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000623091
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
mediator complex
inferred from direct assay
(Park et al., 2003)
(Loncle et al., 2007)
nucleus
inferred from direct assay
(Park et al., 2003)
inferred from direct assay
(assigned by UniProt )
(Leclerc et al., 1996)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cdk8 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Cdk8
Transgenic constructs containing regulatory region of Cdk8
Deletions and Duplications ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal - all die before end of larval stage (with Df(3L)AC1)
lethal - all die before end of larval stage | recessive
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
female sterile, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
Other Phenotypes
Allele
developmental rate defective
neuroanatomy defective | somatic clone | third instar larval stage
some die during third instar larval stage (with Df(3L)AC1)
some die during third instar larval stage | recessive
starvation stress response defective | larval stage | somatic clone, with Scer\GAL4Act.PU
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
anterior pupal spiracle
autophagosome | larval stage | somatic clone, with Scer\GAL4Act.PU
autophagosome | larval stage | somatic clone | conditional, with Scer\GAL4Act.PU
embryo | maternal effect, with Scer\GAL4nos.PG, Scer\GAL4VP16.nos.UTR, Scer\GAL4VP16.otu
embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4Act.PU
embryonic/larval fat body | larval stage | somatic clone | conditional, with Scer\GAL4Act.PU
leg | somatic clone - Minute background
lipid droplet | larval stage | somatic clone | conditional, with Scer\GAL4Act.PU
mesothoracic bristle | somatic clone
mesothoracic bristle | somatic clone - Minute background
mesothoracic tergum, with Scer\GAL4pnr-MD237
prothoracic tarsal segment | somatic clone
sex comb tooth | somatic clone
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\CDK8
14 of 15
Yes
Yes
Hsap\CDK19
12 of 15
Yes
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Cdk8
13 of 15
Yes
Yes
Mmus\Cdk19
12 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Cdk19
9 of 13
Yes
Yes
Rnor\Cdk8
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\cdk8
10 of 12
Yes
Yes
Xtro\cdk19
6 of 12
No
Yes
Danio rerio (Zebrafish) (2)
Drer\cdk8
15 of 15
Yes
Yes
Drer\cdk19
10 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\cdk-8
14 of 15
Yes
Yes
Cele\dyf-18
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (1)
Atha\CDKE;1
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\SSN3
11 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\srb10
10 of 12
Yes
Yes
Spom\ppk23
1 of 12
No
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091908TJ )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Cdk8
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00003907
Drosophila simulans
Dsim\GD12888
Drosophila sechellia
Dsec\GM24836
Drosophila erecta
Dere\GG14001
Drosophila yakuba
Dyak\GE20297
Drosophila ananassae
Dana\GF10227
Drosophila pseudoobscura pseudoobscura
Dpse\GA28311
Drosophila persimilis
Dper\GL20758
Drosophila willistoni
Dwil\GK12315
Drosophila virilis
Dvir\GJ13608
Drosophila mojavensis
Dmoj\GI11413
Drosophila grimshawi
Dgri\GH16999
Drosophila grimshawi
Dgri\GH22186
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091505WJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17006182
Glossina morsitans
Tsetse fly
Gmor\GMOY005245
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_11474
Mayetiola destructor
Hessian fly
Mdes\Mdes007586
Aedes aegypti
Yellow fever mosquito
Aaeg\Cdk8
Anopheles darlingi
American malaria mosquito
Adar\ADAC009784
Anopheles gambiae
Malaria mosquito
Agam\AGAP009936
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ000891
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W05VI )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA011562
Danaus plexippus
Monarch butterfly
Dple\DPOGS212250-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS212251-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL014953
Apis florea
Little honeybee
Aflo\2154238308
Apis mellifera
Western honey bee
Amel\GB45722
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP18853
Bombus terrestris
Buff-tailed bumblebee
Bter\15216556
Linepithema humile
Argentine ant
Lhum\LH23083
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0026b9
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG020283
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_09483
Tribolium castaneum
Red flour beetle
Tcas\TC004891
Pediculus humanus
Human body louse
Phum\PHUM298010
Rhodnius prolixus
Kissing bug
Rpro\RPRC007546
Cimex lectularius
Bed bug
Clec\CLEC003206
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009537
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14179
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05R7 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003235
Ixodes scapularis
Black-legged tick
Isca\ISCW018877
Stegodyphus mimosarum
African social velvet spider
Smim\KFM72363.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur09g05110
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_204007
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_73263
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0812 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Cdk8
Gallus gallus
Domestic chicken
Ggal\CDK19
Gallus gallus
Domestic chicken
Ggal\CDK8
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (14)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Cdc2rk
1 of 10
Cdk1
1 of 10
Cdk2
1 of 10
Cdk4
1 of 10
Cdk5
1 of 10
Cdk7
1 of 10
Cdk9
1 of 10
Cdk12
1 of 10
CG6800
1 of 10
CG7028
1 of 10
CG7236
1 of 10
Eip63E
1 of 10
Pitslre
1 of 10
Pk34A
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    CDK8; cyclin dependent kinase 8
    14 of 15
    603184
        CDK19; cyclin dependent kinase 19
        12 of 15
        614720
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Interactions Browser
            View in Interactions Browser
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Cdk8 - Bap60
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - CG17002
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - CG2017
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - CG3008
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - CG34159
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - Cpsf6
            enzymatic study, autoradiography, experimental knowledge based, anti tag coimmunoprecipitation, anti tag western blot
            (Tang et al., 2018, Guruharsha et al., 2011)
            Cdk8 - CycC
            two hybrid, pull down, autoradiography
            (Loncle et al., 2007)
            Cdk8 - Dif
            pull down, western blot
            (Park et al., 2003)
            Cdk8 - Doa
            anti tag coimmunoprecipitation, anti tag western blot
            (Tang et al., 2018)
            Cdk8 - E2f1
            pull down, anti tag western blot
            (Morris et al., 2008)
            Cdk8 - EcR
            anti bait coimmunoprecipitation, western blot, two hybrid, Identification by mass spectrometry
            (Xie et al., 2015)
            Cdk8 - Hsf
            pull down, western blot
            (Park et al., 2003)
            Cdk8 - IntS1
            anti tag coimmunoprecipitation, western blot
            (Chen et al., 2012)
            Cdk8 - IntS12
            anti bait coimmunoprecipitation, anti tag western blot
            (Chen et al., 2012)
            Cdk8 - IntS9
            anti tag coimmunoprecipitation, western blot
            (Chen et al., 2012)
            Cdk8 - MED1
            anti bait coimmunoprecipitation, western blot
            (Immarigeon et al., 2019)
            Cdk8 - MED11
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED14
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED15
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED16
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED17
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED18
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED20
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED23
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED27
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED30
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED4
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED6
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED7
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - MED8
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - N
            pull down, western blot
            (Park et al., 2003)
            Cdk8 - Psi
            anti bait coimmunoprecipitation, western blot
            (Guo et al., 2016)
            Cdk8 - Ptip
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - Ssdp
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - Usp10
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - arm
            pull down, western blot
            (Park et al., 2003)
            Cdk8 - boca
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - brm
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - dl
            pull down, western blot
            (Park et al., 2003)
            Cdk8 - dsh
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - ebi
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - enc
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - ix
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - kto
            pull down, autoradiography, anti tag coimmunoprecipitation, western blot
            (Chen et al., 2012, Loncle et al., 2007)
            Cdk8 - mor
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - osa
            experimental knowledge based
            (Guruharsha et al., 2011)
            Cdk8 - skd
            experimental knowledge based, pull down, autoradiography, anti tag coimmunoprecipitation, western blot
            (Chen et al., 2012, Guruharsha et al., 2011, Loncle et al., 2007)
            Cdk8 - usp
            anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot
            (Xie et al., 2015)
            Cdk8 - wac
            anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based
            (Rhee et al., 2014)
            Summary of Genetic Interactions
            esyN Network Diagram
            esyN Network Key:
            Suppression
            Enhancement
            View in Interactions Browser
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Cdk8
            suppressible
            Cpsf6
            (Tang et al., 2018)
            Cdk8
            suppressible
            Doa
            (Tang et al., 2018)
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            E2f1
            suppressible
            Cdk8
            (Morris et al., 2008)
            Tsc1|gig
            suppressible
            Cdk8
            (Tang et al., 2018)
            External Data
            Subunit Structure (UniProtKB)
            Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.
            (UniProt, Q9VT57 )
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Gene Group - Pathway Membership (FlyBase)
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            SignaLink - A signaling pathway resource with multi-layered regulatory networks.
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            3L
            Recombination map
            3-31
            Cytogenetic map
            67C10-67C10
            Sequence location
            3L:9,837,080..9,838,670 [-]
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            67C10-67C10
            Limits computationally determined from genome sequence between P{PZ}fry02240 and P{lacW}l(3)L0539L0539&P{PZ}Dhh1rL562
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            67C1-67C11
            (Bennett, 1999.9.10)
            Experimentally Determined Recombination Data
            Location
            3-31
            (FlyBase, 2016)
            3-24.2
            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (10)
            Genomic Clones (12)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (7)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequences
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               
              polyclonal
              (Mazina et al., 2018, Xie et al., 2015)
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of
              Source for identity of: Cdk8 CG10572
              (Li, 1999.11)
              Source for database merge of
              Additional comments
              Other Comments
              dsRNA has been made from templates generated with primers directed against this gene.
              (Marr et al., 2006)
              When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells seen. The proportion of G2/M phase cells is equal to proportion of G1 phase cells.
              (Bettencourt-Dias et al., 2004)
              Transcriptionally downregulated after 2h of insulin stimulation in Kc167 cells. Contains fkh response elements (FHREs) in the genomic upstream or intronic sequences.
              (Junger et al., 2003)
              dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
              (Kiger et al., 2003)
              RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages. This phenotype is not seen in Kc167 cells.
              (Kiger et al., 2003)
              Cdk8 protein interacts in vivo with CycC protein and also with RpII215 protein.
              (Leclerc et al., 1996)
              Interacts in vitro and in vivo with CycC, also can interact in vivo with a large subunit RNA polymerase II.
              (Leopold et al., 1996)
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 38 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
              Other crossreferences
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              SignaLink - A signaling pathway resource with multi-layered regulatory networks.
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyMine - An integrated database for Drosophila genomics
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (9)
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (92)