FlyBase Gene Report: Dmel\Cdk8

General Information

Symbol

Dmel\Cdk8

Species

D. melanogaster

Name

Cyclin-dependent kinase 8

Annotation Symbol

CG10572

Feature Type

protein_coding_gene

FlyBase ID

FBgn0015618

Gene Model Status

Stock Availability

10 publicly available

Enzyme Name (EC)

Cyclin-dependent kinase (2.7.11.22)

[RNA-polymerase]-subunit kinase (2.7.11.23)

Gene Summary

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation. (UniProt, Q9VT57)

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Key Links

Genomic Location

Cytogenetic map

Sequence location

3L:9,837,080..9,838,670 [-]

Recombination map

3-31

RefSeq locus

NT_037436 REGION:9837080..9838670

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (14 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables kinase activity

inferred from direct assay

(Tang et al., 2018)

enables protein binding

inferred from physical interaction with UniProtKB:Q8MSX2

(assigned by UniProt )

(Park et al., 2001)

inferred from physical interaction with UniProtKB:Q7KTX8

(assigned by UniProt )

(Loncle et al., 2007)

inferred from physical interaction with UniProtKB:Q9VW47

(assigned by UniProt )

(Loncle et al., 2007)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

enables cyclin-dependent protein serine/threonine kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

enables RNA polymerase II CTD heptapeptide repeat kinase activity

inferred from biological aspect of ancestor with PANTHER:PTN000623092

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (7 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in chaeta development

inferred from mutant phenotype

(Loncle et al., 2007)

involved_in G1/S transition of mitotic cell cycle

inferred from mutant phenotype

(assigned by UniProt )

(Leclerc et al., 1996)

involved_in imaginal disc-derived leg segmentation

inferred from mutant phenotype

(Loncle et al., 2007)

involved_in positive regulation of autophagy

inferred from mutant phenotype

(Tang et al., 2018)

involved_in protein phosphorylation

inferred from direct assay

(assigned by UniProt )

(Leclerc et al., 1996)

inferred from mutant phenotype

(Sopko et al., 2014)

inferred from direct assay

(Tang et al., 2018)

involved_in sex comb development

inferred from mutant phenotype

(Loncle et al., 2007)

involved_in snRNA 3'-end processing

inferred from direct assay

(Chen et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in protein phosphorylation

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of mediator complex

inferred from direct assay

(Park et al., 2003)

inferred from physical interaction with FLYBASE:kto; FB:FBgn0001324, FLYBASE:skd; FB:FBgn0003415, FLYBASE:CycC; FB:FBgn0004597

(Loncle et al., 2007)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Leclerc et al., 1996)

inferred from direct assay

(Park et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of mediator complex

inferred from biological aspect of ancestor with PANTHER:PTN000623092

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with UniProtKB:P49336

(Boube et al., 2000)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000623091

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES

MEDIATOR COMPLEX

Protein Family (UniProt)

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. (Q9VT57)

Protein Signatures (InterPro)

Catalytic Activity (EC)

Experimental Evidence

-

Predictions / Assertions

ATP + a protein = ADP + a phosphoprotein (2.7.11.22)

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate (2.7.11.23)

Summaries

Gene Group (FlyBase)

CYCLIN DEPENDENT KINASES -: Cyclin-dependent kinases (CDKs) are serine/threonine kinases whose activity depends on a regulatory cyclin subunit. (Adapted from PMID:25180339).
MEDIATOR COMPLEX -: The Mediator (MED) complex serves as a hub for transcriptional signaling events. MED bridges transcriptional activators and repressors. This large multi-protein complex has a central core conserved from yeast to humans. The core is composed of three modules: head, middle, and tail. A separate module, the Cdk8 kinase module, composed of Cdk8, CycC, kto and skd, serves as a regulator. (Adapted from FBrf0226415 and FBrf0191850).

Protein Function (UniProtKB)

Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors. May phosphorylate the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex. Required for leg and eye development and macrochaete specification or differentiation.

(UniProt, Q9VT57)

Summary (Interactive Fly)

Interactive Fly

Gene Model and Products

Number of Transcripts

1

Number of Unique Polypeptides

1

Please see the JBrowse view of Dmel\Cdk8 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VT57)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.45

Gene model reviewed during 5.55

Sequence Ontology: Class of Gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

FBtr0076369

1529

454

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

FBpp0076098

53.7

454

8.87

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.

(UniProt, Q9VT57)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

Q9VT57

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Cdk8 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdk8_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

central brain primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of mediator complex

inferred from direct assay

(Park et al., 2003)

inferred from physical interaction with FLYBASE:kto; FB:FBgn0001324, FLYBASE:skd; FB:FBgn0003415, FLYBASE:CycC; FB:FBgn0004597

(Loncle et al., 2007)

located_in nucleus

inferred from direct assay

(assigned by UniProt )

(Leclerc et al., 1996)

inferred from direct assay

(Park et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Cdk8 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Cdk8

View exonic expression by tissue for Dmel\Cdk8

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015618

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39157/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015618

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015618

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015618

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10572

Flygut - An atlas of the Drosophila adult midgut

FBgn0015618

Images

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 19 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Cdk8

Transgenic constructs containing regulatory region of Cdk8

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

(Loncle et al., 2007)

Df(3L)Cdk8^K145

(Loncle et al., 2007)

Gene partially disrupted in

Df(3L)Cdk8^K185

(Loncle et al., 2007)

Inferred from location ( 0 )

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage, with Cdk8^AQKAA.EGFP

Cdk8^K185

lethal - all die before end of larval stage, with Cdk8^K185

Cdk8^AQKAA.EGFP

lethal - all die before end of larval stage (with Df(3L)AC1)

Cdk8^K185

lethal - all die before end of larval stage | recessive

Cdk8^K185

lethal - all die before end of pupal stage

Cdk8^H2480

lethal - all die during P-stage, with Scer\GAL4^da.G32

Cdk8^HMS05476

lethal - all die during third instar larval stage

Cdk8^K185

lethal - all die during third instar larval stage (with Df(3L)AC1)

Cdk8^K185

partially lethal - majority die, with Scer\GAL4^da.G32

Cdk8^GL00231

partially lethal - majority die, with Scer\GAL4^elav.PLu

Cdk8^GL00231

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Cdk8^GD7022

viable, with Scer\GAL4^Mef2.PR

Cdk8^GD7022

viable, with Scer\GAL4^pnr-MD237

Cdk8^GD7022

viable, with Scer\GAL4^repo

Cdk8^GL00231

viable, with Scer\GAL4^tin.CΔ4

Cdk8^GD7022

Sterility

Allele

female sterile, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Cdk8^GL00231

Other Phenotypes

Allele

abnormal developmental rate

abnormal neuroanatomy | somatic clone | third instar larval stage

Cdk8^H2480

abnormal starvation stress response | larval stage | somatic clone, with Scer\GAL4^Act.PU

Cdk8^GD7022

bang sensitive, with Scer\GAL4^elav.PLu

Cdk8^GL00231

majority die during P-stage, with Scer\GAL4^da.G32

Cdk8^GL00231

short lived, with Scer\GAL4^da.G32

Cdk8^GL00231

short lived, with Scer\GAL4^elav.PLu

Cdk8^GL00231

some die during third instar larval stage (with Df(3L)AC1)

Cdk8^K185

some die during third instar larval stage | recessive

Cdk8^K185

visible, with Scer\GAL4^pnr-MD237

Cdk8^GD7022

Phenotype manifest in

Allele

anterior pupal spiracle

Cdk8^K185

autophagosome | larval stage | somatic clone, with Scer\GAL4^Act.PU

Cdk8^{UAS.ORF.Tag:HA}

autophagosome | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

Cdk8^GD7022

embryo | maternal effect, with Scer\GAL4^nos.PG, Scer\GAL4^VP16.nos.UTR, Scer\GAL4^VP16.otu

Cdk8^GL00231

embryonic/larval fat body | larval stage | somatic clone, with Scer\GAL4^Act.PU

Cdk8^{UAS.ORF.Tag:HA}

embryonic/larval fat body | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

Cdk8^GD7022

leg | somatic clone - Minute background

Cdk8^K185

lipid droplet | larval stage | somatic clone | conditional, with Scer\GAL4^Act.PU

Cdk8^GD7022

mesothoracic bristle | somatic clone

Cdk8^K185

mesothoracic bristle | somatic clone - Minute background

Cdk8^K185

mesothoracic tergum, with Scer\GAL4^pnr-MD237

Cdk8^GD7022

NMJ bouton | decreased number | third instar larval stage, with Scer\GAL4^elav.PLu

Cdk8^GL00231

prothoracic tarsal segment | somatic clone

Cdk8^K185

sex comb tooth | somatic clone

Cdk8^K185

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

13 of 15

Yes

Yes

12 of 15

No

Yes

3

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

12 of 15

Yes

Yes

12 of 15

Yes

Yes

Rattus norvegicus (Norway rat) (2)

10 of 13

Yes

Yes

3 of 13

No

Yes

Xenopus tropicalis (Western clawed frog) (2)

11 of 12

Yes

Yes

7 of 12

No

Yes

Danio rerio (Zebrafish) (2)

14 of 15

Yes

Yes

11 of 15

No

Yes

Caenorhabditis elegans (Nematode, roundworm) (4)

14 of 15

Yes

Yes

2 of 15

No

No

1 of 15

No

No

1 of 15

No

No

Arabidopsis thaliana (thale-cress) (1)

8 of 9

Yes

Yes

Saccharomyces cerevisiae (Brewer's yeast) (3)

11 of 15

Yes

Yes

1 of 15

No

No

1 of 15

No

No

Schizosaccharomyces pombe (Fission yeast) (2)

9 of 12

Yes

Yes

1 of 12

No

No

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (14)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

developmental and epileptic encephalopathy 87

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

model of developmental and epileptic encephalopathy 87

CEA

(Chung et al., 2020)

model of developmental and epileptic encephalopathy 87

CEA

(Chung et al., 2020)

model of developmental and epileptic encephalopathy 87

CEA

(Chung et al., 2020)

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

CDK19; cyclin dependent kinase 19

model of developmental and epileptic encephalopathy 87

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

model of developmental and epileptic encephalopathy 87

is ameliorated by Hsap\CDK19^UAS.Tag:HA

(Chung et al., 2020)

model of developmental and epileptic encephalopathy 87

is ameliorated by Hsap\CDK19^UAS.Tag:HA

(Chung et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

CDK8; cyclin dependent kinase 8

13 of 15

INTELLECTUAL DEVELOPMENTAL DISORDER WITH HYPOTONIA AND BEHAVIORAL ABNORMALITIES; IDDHBA

CDK19; cyclin dependent kinase 19

12 of 15

DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY 87; DEE87

developmental and epileptic encephalopathy 87

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Dmel gene

Ortholog showing functional complementation

Supporting References

(Chung et al., 2020)

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

anti tag coimmunoprecipitation, anti tag western blot, enzymatic study, autoradiography, experimental knowledge based

(Tang et al., 2018, Guruharsha et al., 2011)

pull down, autoradiography, two hybrid

(Loncle et al., 2007)

pull down, western blot

(Park et al., 2003)

anti tag coimmunoprecipitation, anti tag western blot

(Tang et al., 2018)

pull down, anti tag western blot

(Morris et al., 2008)

two hybrid, anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Xie et al., 2015)

pull down, western blot

(Park et al., 2003)

anti tag coimmunoprecipitation, western blot

(Chen et al., 2012)

anti bait coimmunoprecipitation, anti tag western blot

(Chen et al., 2012)

anti tag coimmunoprecipitation, western blot

(Chen et al., 2012)

anti bait coimmunoprecipitation, western blot

(Immarigeon et al., 2019)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, anti tag western blot, two hybrid

(Li et al., 2020)

pull down, western blot

(Park et al., 2003)

anti bait coimmunoprecipitation, western blot

(Guo et al., 2016)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, western blot

(Park et al., 2003)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, western blot

(Park et al., 2003)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, autoradiography, anti tag coimmunoprecipitation, western blot

(Chen et al., 2012, Loncle et al., 2007)

experimental knowledge based

(Guruharsha et al., 2011)

experimental knowledge based

(Guruharsha et al., 2011)

pull down, autoradiography, anti tag coimmunoprecipitation, western blot, experimental knowledge based

(Chen et al., 2012, Guruharsha et al., 2011, Loncle et al., 2007)

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry

(Xie et al., 2015)

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Tang et al., 2018)

suppressible

(Tang et al., 2018)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Morris et al., 2008)

suppressible

(Tang et al., 2018)

External Data

Subunit Structure (UniProtKB)

Component of the Cdk8 module of the Mediator complex, composed of CycC, Cdk8, kto and skd.

(UniProt, Q9VT57 )

Linkouts

BioGRID - A database of protein and genetic interactions.

64544

DroID - A comprehensive database of gene and protein interactions.

FBgn0015618

MIST (protein-protein) - An integrated Molecular Interaction Database

Cdk8

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0015618

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

3L

Recombination map

3-31

Cytogenetic map

Sequence location

3L:9,837,080..9,838,670 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

67C10-67C10

Limits computationally determined from genome sequence between P{PZ}fry⁰²²⁴⁰ and P{lacW}l(3)L0539^L0539&P{PZ}Dhh1^rL562

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

67C1-67C11

(Bennett, 1999.9.10)

Experimentally Determined Recombination Data

Location

3-31

(FlyBase, 2016)

3-24.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (10)

Aberrations including deletions of this gene Aberrations including duplications of this gene

85958

w¹¹¹⁸; PBac{PB}Cdk8^c01804

16058

y¹ w¹¹¹⁸; PBac{5HPw⁺}Cdk8^A162

35324

y¹ sc^* v¹ sev²¹; P{TRiP.GL00231}attP2

67010

y¹ sc^* v¹ sev²¹; P{TRiP.HMS05476}attP40

F001348

M{UAS-Cdk8.ORF}ZH-86Fb

F001713

M{UAS-Cdk8.ORF.3xHA}ZH-86Fb

v107187

P{KK107735}VIE-260B

v31264

w¹¹¹⁸; P{GD7022}v31264

v45370

w¹¹¹⁸; P{GD7022}v45370

v341240

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00282}attP40/CyO-GFP

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (7)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE13344

Other clones

FI03233

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015618

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015618

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39157

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mazina et al., 2018, Xie et al., 2015)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Cdk8 CG10572

(Li, 1999.11)

Source for database merge of

Additional comments

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Marr et al., 2006)

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells seen. The proportion of G2/M phase cells is equal to proportion of G1 phase cells.

(Bettencourt-Dias et al., 2004)

Transcriptionally downregulated after 2h of insulin stimulation in Kc167 cells. Contains fkh response elements (FHREs) in the genomic upstream or intronic sequences.

(Junger et al., 2003)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R⁺ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages. This phenotype is not seen in Kc167 cells.

(Kiger et al., 2003)

Cdk8 protein interacts in vivo with CycC protein and also with RpII215 protein.

(Leclerc et al., 1996)

Interacts in vitro and in vivo with CycC, also can interact in vivo with a large subunit RNA polymerase II.

(Leopold et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39157

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VT57

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VT57

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VT57

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015618

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015618

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39157/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0015618

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Cdk8_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0015618

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0015618

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10572

Flygut - An atlas of the Drosophila adult midgut

FBgn0015618

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0015618

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39157

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004891

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10572

MARRVEL_MODEL - MARRVEL (model organism gene)

39157

modMine - A data warehouse for the modENCODE project

FBgn0015618

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VT57

Linkouts

BioGRID - A database of protein and genetic interactions.

64544

DroID - A comprehensive database of gene and protein interactions.

FBgn0015618

DRSC - Results frm RNAi screens

FBgn0015618

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015618

FlyMine - An integrated database for Drosophila genomics

FBgn0015618

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Cdk8

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CDK8

(Li et al., 2020, Tang et al., 2018, Guo et al., 2016, Joyce et al., 2012, Kondo and Perrimon, 2011, Swaminathan et al., 2010, Bourbon et al., 2004, Boube et al., 2000)

CG10572

(Çiçek et al., 2016, Dahlberg et al., 2015, Hosono et al., 2015, Schertel et al., 2013, Chen et al., 2012, Ni et al., 2011.7.19, Ardehali et al., 2009, Keleman et al., 2009.8.5, Perkins et al., 2009, Berger et al., 2008, Boube et al., 2000, Morrison et al., 2000)

DmCdk8

(Leclerc et al., 1996)

cdk8

(Kuang et al., 2020, Xu et al., 2020, Xie et al., 2015, Berger et al., 2008, Carrera et al., 2008, Brumby et al., 2004)

dCdk8

(Morris et al., 2008)

dTRAP56

(Boube et al., 2000)

p58

(Park et al., 2001)

Name Synonyms

Cyclin-dependent kinase 8

(Perkins et al., 2015, Leopold et al., 1996)

Secondary FlyBase IDs

Datasets (8)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (8)

Result

Result Type

Title

scRNAseq_2020_Cattenoz_NI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from non-infested third instar larvae

scRNAseq_2020_Cattenoz_WI_seq_clustering

cell clustering analysis

Clustering analysis of hemocytes from wasp-infested third instar larvae

scRNAseq_2020_Cho_NI72LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying

scRNAseq_2020_Cho_NI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying

scRNAseq_2020_Cho_WI96LG_seq_clustering

cell clustering analysis

Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI96HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying

scRNAseq_2020_Cho_NI120HL_seq_clustering

cell clustering analysis

Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying

References (103)