l(2)k13315, Nervana, Nrv2.2, Na+/K+ ATPase, l(2)k04223
β subunit of Na,K-ATPase, plays an ion-pump-independent role in junction formation and tracheal morphogenesis - important for maintaining cellular membrane potential in muscles
Please see the JBrowse view of Dmel\nrv2 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
323, 322 (aa); 42 (kD observed)
Nervana (nerve antigen) protein is an anti-HRP
reactive ~42K glycoprotein that is likely to be the major contributor to
neuronal-specific anti-HRP staining. Protein was isolated from adult heads
and monoclonal antibodies were made. Staining with the monoclonal
antibodies reveals two or three products between 38K and 42K. It is
unclear if the properties described apply to nrv1 protein, nrv2
protein, or both.
Nrv 2.1
The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nrv2 using the Feature Mapper tool.
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
The 2.1kb isoform of nrv2 (Nrv2.2) is the major form expressed in fly heads. It is expressed at low levels in the abdomen.
nrv2 is detected in adult head and weakly in thorax by western blot.
nrv2 is nervous system-specific. On western blots of dissected tissues, it is detected in adult heads and thorax, presumably in nervous system components. In adult tissue sections, it is detected predominantly in the nervous system, including the optic lobe, brain, thoracic ganglia, and axonal pathways in the leg.
Nervana protein is detected in adult heads, thoraces and abdomens but is significantly enriched in heads on western blots. On developmental westerns, it is first observed in 6hr embryos and then throughout development. The levels of the different mobility components change through development. The 42K component is not observed until 12-24hr and is not prominent until the adult stage. Nervana protein is observed in the CNS and PNS by immunolocalization in a pattern indistinguishable from the anti-HRP pattern. Staining is also observed in the garland cells and the posterior spiracles.
Comment: exhibits circadian cycling
Comment: exhibits circadian cycling
Comment: exhibits circadian cycling
Comment: in 10-50% of examined hemisegments
Comment: lateral posterior in 10-50% of examined hemisegments
Comment: in 10-50% of examined hemisegments
Comment: in 10-50% of examined hemisegments
Comment: when combined with P{wrapper-GAL4.DBD.932i}
Comment: when combined with P{wrapper-GAL4.DBD.932i}
Comment: when combined with P{wrapper-GAL4.DBD.932i}
Comment: when combined with P{wrapper-GAL4.DBD.932i}
Comment: when combined with P{wrapper-GAL4.DBD.932i}
Comment: when combined with P{wrapper-GAL4.DBD.932i}
GBrowse - Visual display of RNA-Seq signals
View Dmel\nrv2 in GBrowse 22-22
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
monoclonal
Source for identity of: Nrv2 CG9261
Source for identity of: ecta l(2)k04223
Source for merge of: nrv2 l(2)SH0329
Source for merge of: nrv2 NEST:bs13c10
Source for merge of: nrc2 ecta
Source for merge of nrv2 NEST:bs13c10 was sequence comparison ( date:031021 ).
1 allele of nrv2 recovered in a P-insertion screen.
Mutant embryos have lengthened primary tracheal tubes with expanded diameters, and defective septate junctions.
Area matching Drosophila Neuron surface antigen 2 gene, Acc. No. U22439.
Mutation in nrv2 alters tracheal tube size in embryos without affecting other aspects of tracheal development including primary branch budding and outgrowth or branch fusion.
The product(s) of the Nervana gene(s) were recognised by their epitope(s) which reacts with anti-Horse Radish Peroxidase antibodies. These antibodies label neurons in Drosophila. The nac gene product may be required for the glycosylation of these proteins.