FB2020_06, released Dec 22, 2020

Gene: Dmel\nrv2

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General Information
Symbol
Dmel\nrv2
Species
D. melanogaster
Name
nervana 2
Annotation Symbol
CG9261
Feature Type
protein_coding_gene
FlyBase ID
FBgn0015777
Gene Model Status
Current
Stock Availability
21 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

l(2)k13315, Nervana, Nrv2.2, Na+/K+ ATPase, l(2)k04223

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:6,789,770..6,798,862 [-]
Recombination map

2-22

RefSeq locus
NT_033779 REGION:6789770..6798862
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (19 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables ATPase activator activity
inferred from direct assay
(Sun et al., 1998)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables ATPase activator activity
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
contributes_to sodium:potassium-exchanging ATPase activity
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (13 terms)
Terms Based on Experimental Evidence (9 terms)
CV Term
Evidence
References
involved_in cation transport
inferred from direct assay
(Sun et al., 1998)
involved_in cell adhesion involved in heart morphogenesis
inferred from mutant phenotype
(Yi et al., 2008)
involved_in establishment of glial blood-brain barrier
inferred from mutant phenotype
(Stork et al., 2008, Schwabe et al., 2005)
involved_in open tracheal system development
inferred from mutant phenotype
(Beitel and Krasnow, 2000)
involved_in regulation of tube diameter, open tracheal system
inferred from mutant phenotype
(Paul et al., 2003)
involved_in regulation of tube length, open tracheal system
inferred from mutant phenotype
(Paul et al., 2003)
involved_in regulation of tube size, open tracheal system
inferred from mutant phenotype
(Beitel and Krasnow, 2000)
involved_in sensory perception of sound
inferred from mutant phenotype
(Roy et al., 2013)
involved_in septate junction assembly
inferred from mutant phenotype
(Paul et al., 2003)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in cellular potassium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in cellular sodium ion homeostasis
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in potassium ion import across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in sodium ion export across plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Lowe et al., 2014)
located_in plasma membrane
inferred from direct assay
(Yi et al., 2008)
inferred from high throughput direct assay
(Tan et al., 2009)
located_in septate junction
inferred from direct assay
(Paul et al., 2007, Genova and Fehon, 2003)
part_of sodium:potassium-exchanging ATPase complex
inferred from direct assay
(Sun et al., 1998)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
part_of sodium:potassium-exchanging ATPase complex
inferred from biological aspect of ancestor with PANTHER:PTN000897489
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the X(+)/potassium ATPases subunit beta family. (Q24048)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
NA+/K+ TRANSPORTING P-ATPASE - BETA SUBUNITS -
Na+/K+ transporting ATPase β-subunit is a type II membrane protein required for the structural and functional maturation of the α-subunit and membrane targeting. (Adapted from FBrf0210681).
Protein Function (UniProtKB)
This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
(UniProt, Q24048)
Summary (Interactive Fly)

β subunit of Na,K-ATPase, plays an ion-pump-independent role in junction formation and tracheal morphogenesis - important for maintaining cellular membrane potential in muscles

Gene Model and Products
Number of Transcripts
6
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\nrv2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.45

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
nrv2-RA
FBtr0079382
NM_057821
1980
322
nrv2-RC
FBtr0079381
NM_057820
2668
323
nrv2-RD
FBtr0079383
NM_164711
2156
322
nrv2-RE
FBtr0100381
NM_001014476
1949
322
nrv2-RF
FBtr0100382
NM_001014475
2143
323
nrv2-RG
FBtr0332369
NM_001273235
2635
323
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
nrv2-PA
FBpp0079010
37.0
322
8.03
NP_477169
AAF52438
nrv2-PC
FBpp0079009
37.3
323
7.39
NP_477168
AAN10600
nrv2-PD
FBpp0079011
37.0
322
8.03
NP_723216
AAF52439
nrv2-PE
FBpp0099793
37.0
322
8.03
NP_001014476
AAX52660
nrv2-PF
FBpp0099794
37.3
323
7.39
NP_001014475
AAX52659
nrv2-PG
FBpp0304645
37.3
323
7.39
NP_001260164
AGB92700
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

322 aa isoforms: nrv2-PA, nrv2-PD, nrv2-PE
323 aa isoforms: nrv2-PC, nrv2-PF, nrv2-PG
Additional Polypeptide Data and Comments
Reported size (kDa)

323, 322 (aa); 42 (kD observed)

(Sun and Salvaterra, 1995)
Comments

Nervana (nerve antigen) protein is an anti-HRP

reactive ~42K glycoprotein that is likely to be the major contributor to

neuronal-specific anti-HRP staining. Protein was isolated from adult heads

and monoclonal antibodies were made. Staining with the monoclonal

antibodies reveals two or three products between 38K and 42K. It is

unclear if the properties described apply to nrv1 protein, nrv2

protein, or both.

(Sun et al., 1998)

nrv1 protein and/or nrv2 protein can be copurified with the α subunit of Drosophila Na+,K+-ATPase from extracts of Drosophila heads.

(Sun and Salvaterra, 1995)

Nrv 2.1

(FlyBase, 1992-)
External Data
Subunit Structure (UniProtKB)

The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.

(UniProt, Q24048)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\nrv2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 7 -- 8
antennal anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
central brain anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
visual anlage

Comment: reported as procephalic ectoderm anlage

(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
dorsal epidermis primordium
(Fisher et al., 2012)
foregut primordium
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic central brain surface glia
(Fisher et al., 2012)
embryonic dorsal epidermis
(Fisher et al., 2012)
embryonic foregut
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
lateral cord surface glial cell
(Fisher et al., 2012)
sensory nervous system primordium
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
medial-most cell body glial cell
(Kearney et al., 2004)
RNase protection, primer extension, SI map
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
abdomen
(Sun et al., 1998)
(Sun et al., 1998)
adult head
(Sun et al., 1998)
adult abdomen
(Sun et al., 1998)
Additional Descriptive Data

The 2.1kb isoform of nrv2 (Nrv2.2) is the major form expressed in fly heads. It is expressed at low levels in the abdomen.

(Sun et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
dissected tissue
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Sun et al., 1998)
adult thorax
(Sun et al., 1998)
adult optic lobe
(Sun et al., 1998)
adult ventral nervous system
(Sun et al., 1998)
adult brain
(Sun et al., 1998)
leg
(Sun et al., 1998)
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
longitudinal connective
(Sun and Salvaterra, 1995)
adult stage
scolopale cell of Johnston organ
(Roy et al., 2013)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
central nervous system
(Sun and Salvaterra, 1995)
commissure
(Sun and Salvaterra, 1995)
embryonic/larval posterior spiracle
(Sun and Salvaterra, 1995)
presumptive embryonic/larval peripheral nervous system
(Sun and Salvaterra, 1995)
embryonic/larval garland cell
(Sun and Salvaterra, 1995)
adult stage
adult thorax
(Baumann et al., 2010, Sun and Salvaterra, 1995)
adult head
(Baumann et al., 2010, Sun and Salvaterra, 1995)
adult abdomen
(Sun and Salvaterra, 1995)
embryonic stage -- adult stage
head
(Sun and Salvaterra, 1995)
Additional Descriptive Data

nrv2 is detected in adult head and weakly in thorax by western blot.

(Baumann et al., 2010)

nrv2 is nervous system-specific. On western blots of dissected tissues, it is detected in adult heads and thorax, presumably in nervous system components. In adult tissue sections, it is detected predominantly in the nervous system, including the optic lobe, brain, thoracic ganglia, and axonal pathways in the leg.

(Sun et al., 1998)

Nervana protein is detected in adult heads, thoraces and abdomens but is significantly enriched in heads on western blots. On developmental westerns, it is first observed in 6hr embryos and then throughout development. The levels of the different mobility components change through development. The 42K component is not observed until 12-24hr and is not prominent until the adult stage. Nervana protein is observed in the CNS and PNS by immunolocalization in a pattern indistinguishable from the anti-HRP pattern. Staining is also observed in the garland cells and the posterior spiracles.

(Sun and Salvaterra, 1995)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in axon
inferred from direct assay
(Lowe et al., 2014)
located_in plasma membrane
inferred from direct assay
(Yi et al., 2008)
inferred from high throughput direct assay
(Tan et al., 2009)
located_in septate junction
inferred from direct assay
(Paul et al., 2007, Genova and Fehon, 2003)
part_of sodium:potassium-exchanging ATPase complex
inferred from direct assay
(Sun et al., 1998)
Expression Deduced from Reporters
Reporter: P{nrv2-GAL4.S}
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage -- adult stage
neuron | subset & glial cell | subset of central nervous system
(Sun et al., 2001)
embryonic stage -- adult stage
nervous system
(Sun et al., 1999)
neuron
(Sun et al., 1999)
third instar larval stage
embryonic/larval wrapping glial cell
(Coutinho-Budd et al., 2017, Schmidt et al., 2012)
embryonic/larval cell body glial cell
(Coutinho-Budd et al., 2017)
astrocyte-like glial cell
(Coutinho-Budd et al., 2017)
embryonic/larval ensheathing glial cell
(Coutinho-Budd et al., 2017)
larval stage
embryonic/larval glial cell | subset of embryonic/larval optic lobe
(Edwards et al., 2012)
pupal stage P7
developing satellite glial cell | subset
(Edwards et al., 2012)
pupal stage
glial cell | subset of adult optic lobe
(Edwards et al., 2012)
pharate adult stage -- adult stage
adult distal lamina satellite glial cell
(Edwards et al., 2012)
adult stage
adult optic lobe

Comment: exhibits circadian cycling

(Górska-Andrzejak et al., 2009)
lamina

Comment: exhibits circadian cycling

(Górska-Andrzejak et al., 2009)
medulla

Comment: exhibits circadian cycling

(Górska-Andrzejak et al., 2009)
glial cell | subset of adult optic lobe
(Shimozono et al., 2019, Górska-Andrzejak et al., 2009)
adult lamina satellite glial cell
(Górska-Andrzejak et al., 2009)
adult inner optic chiasma giant glial cell
(Górska-Andrzejak et al., 2009)
adult outer optic chiasma giant glial cell
(Górska-Andrzejak et al., 2009)
adult medulla neuropil associated glial cell
(Górska-Andrzejak et al., 2009)
adult cerebrum
(Górska-Andrzejak et al., 2009)
glial cell of antennal lobe
(Oland et al., 2008)
embryonic stage 16
dorsal channel glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
longitudinal glial cell

Comment: lateral posterior in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
medial intersegmental nerve root glial cell
(Beckervordersandforth et al., 2008)
lateral segmental nerve root glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
medial-most cell body glial cell
(Beckervordersandforth et al., 2008)
medial cell body glial cell
(Beckervordersandforth et al., 2008)
lateral cell body glial cell

Comment: in 10-50% of examined hemisegments

(Beckervordersandforth et al., 2008)
Reporter: P{nrv2-GAL4.U}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval wrapping glial cell
(Otto et al., 2018)
embryonic/larval cell body glial cell
(Otto et al., 2018)
embryonic/larval ensheathing glial cell
(Otto et al., 2018)
embryonic/larval astrocyte-like glial cell
(Otto et al., 2018)
Reporter: P{nrv2-lacZ.7.7}
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 17
embryonic/larval central nervous system
(Xu et al., 1999)
embryonic/larval peripheral nervous system
(Xu et al., 1999)
adult stage
adult brain
(Xu et al., 1999)
adult ventral nervous system
(Xu et al., 1999)
supraesophageal ganglion
(Xu et al., 1999)
adult gnathal ganglion
(Xu et al., 1999)
adult optic lobe
(Xu et al., 1999)
adult optic lobe
(Xu et al., 1999)
Reporter: P{nrv2-VP16.AD}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
embryonic stage 12
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
embryonic stage 15
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
first instar larval stage
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
second instar larval stage
embryonic/larval cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
adult stage
adult brain cell body glial cell

Comment: when combined with P{wrapper-GAL4.DBD.932i}

(Coutinho-Budd et al., 2017)
Reporter: P{PTT-GA}nrv2ZCL1649
Stage
Tissue/Position (including subcellular localization)
Reference
oogenesis & adult stage | female
follicle cell
(Cooley et al., 2002.10-2003.5)
oocyte
(Cooley et al., 2002.10-2003.5)
nurse cell
(Cooley et al., 2002.10-2003.5)
Reporter: P{PTT-un}Nrv2PTT
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 16
ectoderm | restricted
(Edenfeld et al., 2006)
embryonic/larval nervous system | restricted
(Edenfeld et al., 2006)
Reporter: P{PTT-un}nrv2ZCL2903
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage | male
cyst cell of testis
(Cash and Andrews, 2012)
adult stage | female
germarium | restricted
(Cash and Andrews, 2012)
Reporter: P{unk}nrv2173
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
glial cell | subset of embryonic/larval peripheral nervous system
(Stork et al., 2008)
Reporter: PBac{681.P.FSVS-1}nrv2CPTI001455
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult brain cell body rind
(Knowles-Barley, 2011.8.24)
Reporter: PBac{CPTI-un}nrv2CPTI001459
Stage
Tissue/Position (including subcellular localization)
Reference
first instar larval stage
motor neuron
• axon

Comment: axons

(Lowe et al., 2014)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\nrv2 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 19 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of nrv2
Transgenic constructs containing regulatory region of nrv2
Deletions and Duplications ( 5 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (7)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\ATP1B1
12 of 15
Yes
No
Hsap\ATP1B2
10 of 15
No
No
Hsap\ATP1B4
10 of 15
No
Yes
Hsap\ATP1B3
9 of 15
No
No
Hsap\ATP4B
6 of 15
No
No
Hsap\KNG1
1 of 15
No
Yes
Hsap\SHBG
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Atp1b1
11 of 15
Yes
No
Mmus\Atp1b2
10 of 15
No
No
Mmus\Atp1b4
10 of 15
No
Yes
Mmus\Atp1b3
8 of 15
No
No
Mmus\Atp4b
7 of 15
No
No
Mmus\Shbg
1 of 15
No
Yes
Rattus norvegicus (Norway rat) (6)
Rnor\Atp1b1
11 of 13
Yes
No
Rnor\Atp1b2
10 of 13
No
No
Rnor\Atp1b4
9 of 13
No
Yes
Rnor\Atp1b3
8 of 13
No
Yes
Rnor\Atp4b
8 of 13
No
Yes
Rnor\Shbg
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
Xtro\atp1b1
11 of 12
Yes
No
Xtro\atp1b2
7 of 12
No
No
Xtro\atp4b
7 of 12
No
No
Xtro\atp1b3
6 of 12
No
Yes
Xtro\atp1b4
6 of 12
No
Yes
Danio rerio (Zebrafish) (8)
Drer\atp1b1a
11 of 15
Yes
No
Drer\atp1b2b
10 of 15
No
No
Drer\atp1b3a
10 of 15
No
No
Drer\atp1b4
10 of 15
No
Yes
Drer\atp1b3b
9 of 15
No
No
Drer\atp1b1b
8 of 15
No
No
Drer\atp1b2a
8 of 15
No
No
Drer\shbg
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (3)
Cele\nkb-3
9 of 15
Yes
No
Cele\nkb-1
8 of 15
No
No
Cele\nkb-2
6 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190AEW )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00001455
Drosophila simulans
Dsim\GD22540
Drosophila sechellia
Dsec\GM13825
Drosophila erecta
Dere\GG23580
Drosophila yakuba
Dyak\GE18402
Drosophila ananassae
Dana\GF14479
Drosophila pseudoobscura pseudoobscura
Dpse\GA21652
Drosophila persimilis
Dper\GL25960
Drosophila willistoni
Dwil\GK24210
Drosophila virilis
Dvir\GJ15658
Drosophila mojavensis
Dmoj\GI17298
Drosophila grimshawi
Dgri\GH10786
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506TY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17010682
Glossina morsitans
Tsetse fly
Gmor\GMOY002427
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_07903
Mayetiola destructor
Hessian fly
Mdes\Mdes009961
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL010145
Anopheles darlingi
American malaria mosquito
Adar\ADAC001447
Anopheles gambiae
Malaria mosquito
Agam\AGAP007791
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ007116
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W09EO )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA006099
Bombyx mori
Silkmoth
Bmor\BGIBMGA006060
Bombyx mori
Silkmoth
Bmor\BGIBMGA006062
Bombyx mori
Silkmoth
Bmor\BGIBMGA006057
Danaus plexippus
Monarch butterfly
Dple\DPOGS201882-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201884-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201847-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS201880-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL013099
Heliconius melpomene
Postman butterfly
Hmel\HMEL013094
Heliconius melpomene
Postman butterfly
Hmel\HMEL013096
Heliconius melpomene
Postman butterfly
Hmel\HMEL013095
Apis florea
Little honeybee
Aflo\4691336228
Apis florea
Little honeybee
Aflo\2578563636
Apis florea
Little honeybee
Aflo\2578563684
Apis mellifera
Western honey bee
Amel\GB46146
Apis mellifera
Western honey bee
Amel\LOC408722
Apis mellifera
Western honey bee
Amel\GB46119
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11656
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11717
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP11657
Bombus terrestris
Buff-tailed bumblebee
Bter\15204042
Bombus terrestris
Buff-tailed bumblebee
Bter\15204036
Bombus terrestris
Buff-tailed bumblebee
Bter\15204043
Linepithema humile
Argentine ant
Lhum\LH16951
Linepithema humile
Argentine ant
Lhum\LH16954
Linepithema humile
Argentine ant
Lhum\LH16949
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001ada
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00222c
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001adb
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG006932
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG006935
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG006937
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_04869
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10242
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00246
Tribolium castaneum
Red flour beetle
Tcas\TC014031
Tribolium castaneum
Red flour beetle
Tcas\TC010394
Tribolium castaneum
Red flour beetle
Tcas\TC033052
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_15676
Tribolium castaneum
Red flour beetle
Tcas\TC014033
Pediculus humanus
Human body louse
Phum\PHUM583400
Pediculus humanus
Human body louse
Phum\PHUM583290
Pediculus humanus
Human body louse
Phum\PHUM411340
Pediculus humanus
Human body louse
Phum\PHUM465330
Rhodnius prolixus
Kissing bug
Rpro\RPRC005169
Rhodnius prolixus
Kissing bug
Rpro\RPRC005314
Rhodnius prolixus
Kissing bug
Rpro\RPRC013435
Rhodnius prolixus
Kissing bug
Rpro\RPRC015395
Rhodnius prolixus
Kissing bug
Rpro\RPRC005313
Cimex lectularius
Bed bug
Clec\CLEC007137
Cimex lectularius
Bed bug
Clec\CLEC005621
Cimex lectularius
Bed bug
Clec\CLEC007141
Cimex lectularius
Bed bug
Clec\CLEC007138
Cimex lectularius
Bed bug
Clec\CLEC005226
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006475
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009444
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03642
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04988
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03640
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_03643
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X09B2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR001877
Ixodes scapularis
Black-legged tick
Isca\ISCW016921
Ixodes scapularis
Black-legged tick
Isca\ISCW013836
Stegodyphus mimosarum
African social velvet spider
Smim\KFM82396.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM76729.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur35g00730
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_230473
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_93126
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0EKJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Atp11
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (6)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
nrv1
7 of 10
nrv3
6 of 10
CG11703
5 of 10
CG33310
5 of 10
CG5250
3 of 10
GABA-B-R1
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    ATP1B1; ATPase Na+/K+ transporting subunit beta 1
    model of  essential hypertension
    IEA
    (FlyBase, 2019-)
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    nrv2k13315
    exacerbates  Huntington's disease
    modeled by Hsap\HTT128Q.1-336.UAS
    (Shirasaki et al., 2012)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ATP1B1; ATPase Na+/K+ transporting subunit beta 1
    12 of 15
    182330
    ATP1B2; ATPase Na+/K+ transporting subunit beta 2
    10 of 15
    182331
        ATP1B4; ATPase Na+/K+ transporting family member beta 4
        10 of 15
            ATP1B3; ATPase Na+/K+ transporting subunit beta 3
            9 of 15
            601867
                ATP4B; ATPase H+/K+ transporting subunit beta
                6 of 15
                137217
                    Functional Complementation Data
                    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
                    Interactions
                    Summary of Physical Interactions
                    esyN Network Diagram
                    Show neighbor-neighbor interactions:
                    Select Layout:
                    Legend:
                    Protein
                    RNA
                    Selected Interactor(s)
                    Interactions Browser
                    View in Interactions Browser
                    Please see the Physical Interaction reports below for full details
                    protein-protein
                    Physical Interaction
                    Assay
                    References
                    nrv2 - Atpα
                    experimental knowledge based
                    (Guruharsha et al., 2011)
                    nrv2 - CG4872
                    experimental knowledge based
                    (Guruharsha et al., 2011)
                    nrv2 - NKAIN
                    anti tag coimmunoprecipitation, western blot
                    (Gorokhova et al., 2007)
                    nrv2 - Nrx-IV
                    anti bait coimmunoprecipitation, western blot
                    (Genova and Fehon, 2003)
                    nrv2 - cora
                    anti bait coimmunoprecipitation, western blot
                    (Genova and Fehon, 2003)
                    Summary of Genetic Interactions
                    esyN Network Diagram
                    esyN Network Key:
                    Suppression
                    Enhancement
                    View in Interactions Browser
                    Please look at the allele data for full details of the genetic interactions
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    nrv2
                    suppressible
                    nrv2::nrv3
                    (Paul et al., 2007)
                    Starting gene(s)
                    Interaction type
                    Interacting gene(s)
                    Reference
                    External Data
                    Subunit Structure (UniProtKB)
                    The sodium/potassium-transporting ATPase is composed of a catalytic alpha subunit, an auxiliary non-catalytic beta subunit and an additional regulatory subunit.
                    (UniProt, Q24048 )
                    Linkouts
                    DroID - A comprehensive database of gene and protein interactions.
                    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Pathways
                    Signaling Pathways (FlyBase)
                    Metabolic Pathways
                    External Data
                    Linkouts
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    Genomic Location and Detailed Mapping Data
                    Chromosome (arm)
                    2L
                    Recombination map

                    2-22

                    Cytogenetic map
                    27B1-27B2
                    Sequence location
                    2L:6,789,770..6,798,862 [-]
                    FlyBase Computed Cytological Location
                    Cytogenetic map
                    Evidence for location
                    27B1-27B2
                    Experimentally Determined Cytological Location
                    Cytogenetic map
                    Notes
                    References
                    26F7-27A1
                    (Oh et al., 2003)
                    27B1-27B2
                    (determined by in situ hybridisation)
                    (Beitel and Krasnow, 2000, Spradling et al., 1999, Williamson et al., 1998)
                    27A-27B
                    (determined by in situ hybridisation)
                    (Salveterra, 1999.1.20)
                    92C-92D
                    (determined by in situ hybridisation)
                    (Sun and Salvaterra, 1995)
                    27B1-27B2
                    (BDGP Project Members, 1994-1999)
                    Experimentally Determined Recombination Data
                    Location

                    2-22

                    (FlyBase, 2016)

                    2-21.6

                    (Comeron et al., 2012)

                    2-

                    Left of (cM)
                    Right of (cM)
                    Notes
                    Stocks and Reagents
                    Stocks (21)
                    Genomic Clones (21)
                    cDNA Clones (160)
                     

                    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

                    cDNA clones, fully sequenced
                    BDGP DGC clones
                    Other clones
                    Drosophila Genomics Resource Center cDNA clones

                    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

                    cDNA Clones, End Sequenced (ESTs)
                    BDGP DGC clones
                    Other clones
                    RNAi and Array Information
                    Linkouts
                    DRSC - Results frm RNAi screens
                    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                    Antibody Information
                    Laboratory Generated Antibodies
                     

                    polyclonal

                    (Sun et al., 1998)

                    monoclonal

                    (Sun and Salvaterra, 1995)
                    Commercially Available Antibodies
                     
                    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
                    Other Information
                    Relationship to Other Genes
                    Source for database identify of

                    Source for identity of: Nrv2 CG9261

                    (Wortman, 1999.11)

                    Source for identity of: ecta l(2)k04223

                    (Beitel and Krasnow, 2000)
                    Source for database merge of

                    Source for merge of: nrv2 l(2)SH0329

                    (FlyBase, 1992-)

                    Source for merge of: nrv2 NEST:bs13c10

                    (Paul et al., 2003, FlyBase, 1996-)

                    Source for merge of: nrc2 ecta

                    (Paul et al., 2003)
                    Additional comments

                    Source for merge of nrv2 NEST:bs13c10 was sequence comparison ( date:031021 ).

                    (FlyBase, 1996-)
                    Other Comments

                    1 allele of nrv2 recovered in a P-insertion screen.

                    (Oh et al., 2003)

                    Mutant embryos have lengthened primary tracheal tubes with expanded diameters, and defective septate junctions.

                    (Paul et al., 2003)

                    Area matching Drosophila Neuron surface antigen 2 gene, Acc. No. U22439.

                    (Davies, 2001.3.15)

                    Mutation in nrv2 alters tracheal tube size in embryos without affecting other aspects of tracheal development including primary branch budding and outgrowth or branch fusion.

                    (Beitel and Krasnow, 2000)

                    The product(s) of the Nervana gene(s) were recognised by their epitope(s) which reacts with anti-Horse Radish Peroxidase antibodies. These antibodies label neurons in Drosophila. The nac gene product may be required for the glycosylation of these proteins.

                    (Sun and Salvaterra, 1995)

                    Southern blot of cRNA probes hybridised to genomic DNA indicates nrv1 and nrv2 are derived from different genes.

                    (Sun and Salvaterra, 1995)

                    nrv1 and nrv2 are neuron-specific forms of the β subunit of Na+,K+-ATPase.

                    (Sun and Salvaterra, 1995)
                    Origin and Etymology
                    Discoverer
                    Etymology
                    Identification
                    External Crossreferences and Linkouts ( 70 )
                    Sequence Crossreferences
                    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                    UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
                    Other crossreferences
                    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
                    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                    Flygut - An atlas of the Drosophila adult midgut
                    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
                    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                    InterPro - A database of protein families, domains and functional sites
                    KEGG Genes - Molecular building blocks of life in the genomic space.
                    modMine - A data warehouse for the modENCODE project
                    Linkouts
                    DPiM - Drosophila Protein interaction map
                    DroID - A comprehensive database of gene and protein interactions.
                    DRSC - Results frm RNAi screens
                    Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
                    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
                    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                    FlyMine - An integrated database for Drosophila genomics
                    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
                    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
                    MIST (protein-protein) - An integrated Molecular Interaction Database
                    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                    Synonyms and Secondary IDs (29)
                    Reported As
                    Symbol Synonym
                    42kDa
                    (Pahud et al., 1998)
                    CG9261
                    (Meadows, 2017.1.11, Çiçek et al., 2016, Jaspers et al., 2012, Cammarato et al., 2011, Ling and Salvaterra, 2011, Ni et al., 2011.7.19, Khanna et al., 2010, Perkins et al., 2009, Schiess et al., 2009, Quinones-Coello, 2007, Rendic et al., 2006)
                    NEST:bs13c10
                    Na,K-ATPase
                    (Okamura et al., 2001)
                    Na+/K+ ATPase
                    (Chen and Haddad, 2004)
                    Na+/K+-ATPase
                    (Linton and O'Donnell, 1999)
                    Na+/K+ATPase
                    (Chen et al., 2002)
                    NaK ATPase
                    (Wu and Beitel, 2003)
                    Nrv
                    Nrv2
                    (Lim et al., 2019, Spéder and Brand, 2018, Lowe et al., 2014, Chen and Zhang, 2013, Górska-Andrzejak, 2013, Górska-Andrzejak et al., 2009, Larsen et al., 2009, Fung et al., 2008, Oland et al., 2008, Paul et al., 2007, Altenhein et al., 2006, Wang et al., 2004, Cooley et al., 2002.10-2003.5, Bellen, 2000.8.28, Prokop, 1999, Emery et al., 1998, Sun et al., 1998)
                    Nrv2.2
                    (Dalla et al., 2017, Dalla and Dobler, 2016, Gorokhova et al., 2007)
                    ecta
                    l(2)SH0329
                    l(2)SH2 0329
                    (Oh et al., 2002.3.22)
                    l(2)k04223
                    (Spradling et al., 1999)
                    l(2)k13315
                    (Kaufmann et al., 2002, Spradling et al., 1999)
                    nrv2
                    (Hope et al., 2020, Miller and Posakony, 2020, Ariss et al., 2018, Croset et al., 2018, Daniel et al., 2018, Charlton-Perkins et al., 2017, Altenhein et al., 2016, Sarov et al., 2016, Gene Disruption Project members, 2015-, Lin et al., 2015, DeSalvo et al., 2014, Fedotov et al., 2014, Hall et al., 2014, Kerr et al., 2014, Luan et al., 2014, Ma and Weake, 2014, Mukherjee et al., 2014, Robbins et al., 2014, Roy et al., 2013, von Hilchen et al., 2013, Cash and Andrews, 2012, Japanese National Institute of Genetics, 2012.5.21, Nelson et al., 2012, Shirasaki et al., 2012, Friedman et al., 2011, Guan et al., 2011, Ling and Salvaterra, 2011, Xie and Auld, 2011, Baumann et al., 2010, Nilton et al., 2010, Spindler et al., 2009, Stork et al., 2009, Tan et al., 2009, Quijano et al., 2008, Stork et al., 2008, Yi et al., 2008, Baer et al., 2007, Dietzl et al., 2007, Li et al., 2007, Paul et al., 2007, Quinones-Coello, 2007, Wu et al., 2007, Tolhuis et al., 2006, Younossi-Hartenstein et al., 2006, Schwabe et al., 2005)
                    nrv2.2
                    (Rendic et al., 2006)
                    Name Synonyms
                    Na+/K+ ATPase
                    (Nilton et al., 2010)
                    Na,K-ATPase β-subunit
                    (Dalla and Dobler, 2016)
                    Na+/K+ ATPase
                    (Ling and Salvaterra, 2011)
                    Nervana
                    (Beramendi et al., 2007, Rendic et al., 2006, Genova and Fehon, 2001)
                    Nervana 2
                    (Lowe et al., 2014, Chen and Zhang, 2013, Górska-Andrzejak, 2013, Guan et al., 2011, Xie and Auld, 2011, Khanna et al., 2010, Górska-Andrzejak et al., 2009, Tan et al., 2009)
                    Nervana2
                    (Rodrigues et al., 2011, Oland et al., 2008, Roy et al., 2007)
                    Nirvana
                    ectatic
                    (Beitel and Krasnow, 2000)
                    nervana
                    (Sun and Salvaterra, 1995)
                    nervana 2
                    (Saito et al., 2018, Cash and Andrews, 2012, Jaspers et al., 2012, Cammarato et al., 2011, Nilton et al., 2010, Baer et al., 2007)
                    nervana2
                    (Stork et al., 2008, Yi et al., 2008, Nair et al., 2007, Morin et al., 2001)
                    nirvana2
                    (Spindler et al., 2009)
                    Secondary FlyBase IDs
                    • FBgn0014867
                    • FBgn0021885
                    • FBgn0022206
                    • FBgn0045319
                    • FBgn0065838
                    Datasets (0)
                    Study focus (0)
                    Experimental Role
                    Project
                    Project Type
                    Title
                    References (177)