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Gene Dmel\nrv2

General Information
Symbol	Dmel\nrv2	Species	D. melanogaster
Name	nervana 2	Annotation symbol	CG9261
Feature type	protein_coding_gene	FlyBase ID	FBgn0015777
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	l(2)k13315, nrv2.2
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	27B1-27B2	Sequence location	2L:6,789,770..6,798,862 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene nervana 2 is referred to in FlyBase by the symbol Dmel\nrv2 (CG9261, FBgn0015777). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: sodium:potassium-exchanging ATPase activity; cation transmembrane transporter activity. There is experimental evidence that it is involved in the biological process: regulation of tube diameter, open tracheal system; regulation of tube size, open tracheal system; establishment of glial blood-brain barrier; open tracheal system development; regulation of tube length, open tracheal system; cation transport; septate junction assembly. 28 alleles are reported. The phenotypes of these alleles are annotated with: tracheal branch primordium; presumptive embryonic salivary gland; trichogen cell; embryonic somatic muscle; embryonic/larval dorsal trunk; septate junction; embryonic/larval tracheal system; dorsal trunk primordium; embryonic/larval transverse connective; ganglionic branch primordium. It has 6 annotated transcripts and 6 annotated polypeptides. Protein features are: Sodium/potassium-transporting ATPase subunit beta. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-24 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in adult male stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval/adult hindgut, adult heart, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625215_s_at completely aligns to an exonic region common to each of the 5 FlyBase-annotated transcript isoforms of nrv2. Gene sequence location is 2L:6789770..6798862.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms sensory perception of sound
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 27B1-27B2
Experimentally Determined Cytological Location
Cytogenetic map Notes References 26F7-27A1     (Oh et al., 2003) 27B1-27B2   (determined by in situ hybridisation)   (Beitel and Krasnow, 2000) 27B1-27B2   (determined by in situ hybridisation)   (Spradling et al., 1999) 27A-27B   (determined by in situ hybridisation)   (Salveterra, 1999.1.20) 27B1-27B2   (determined by in situ hybridisation)   (Williamson et al., 1998) 92C-92D   (determined by in situ hybridisation)   (Sun and Salvaterra, 1995) 27B1-27B2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2-
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\nrv2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45 Annotated transcripts do not represent all supported alternative splices within 5' UTR. Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) nrv2-RA FBtr0079382  NM_057821   1980   322 nrv2-RC FBtr0079381  NM_057820   2668   323 nrv2-RD FBtr0079383  NM_164711   2156   322 nrv2-RE FBtr0100381  NM_001014476   1949   322 nrv2-RF FBtr0100382  NM_001014475   2143   323 nrv2-RG FBtr0332369  NM_001273235   2635   323
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein nrv2-PA   FBpp0079010   37.0   322   8.03     NP_477169   AAF52438 nrv2-PC   FBpp0079009   37.3   323   7.39     NP_477168   AAN10600 nrv2-PD   FBpp0079011   37.0   322   8.03     NP_723216   AAF52439 nrv2-PE   FBpp0099793   37.0   322   8.03     NP_001014476   AAX52660 nrv2-PF   FBpp0099794   37.3   323   7.39     NP_001014475   AAX52659 nrv2-PG   FBpp0304645   37.3   323   7.39     NP_001260164   AGB92700
Additional Polypeptide Data & Comments
Reported size (kDa)	323, 322 (aa); 42 (kD observed) (Sun and Salvaterra, 1995)
Comments	Nervana (nerve antigen) protein is an anti-HRP reactive ~42K glycoprotein that is likely to be the major contributor to neuronal-specific anti-HRP staining. Protein was isolated from adult heads and monoclonal antibodies were made. Staining with the monoclonal antibodies reveals two or three products between 38K and 42K. It is unclear if the properties described apply to nrv1 protein, nrv2 protein, or both. (Sun et al., 1998) nrv1 protein and/or nrv2 protein can be copurified with the α subunit of Drosophila Na+,K+-ATPase from extracts of Drosophila heads. (Sun and Salvaterra, 1995, Sun and Salvaterra, 1995) Nrv 2.1 (FlyBase, 1992-) Nrv 2.2 (FlyBase, 1992-)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sodium/potassium-transporting ATPase subunit beta (IPR000402)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141014   AA141761   AA439276   AE014134 AAF52438   AE014134 AAF52439   AE014134 AAN10600   AE014134 AAX52659   AE014134 AAX52660   AB081999   AC004303   AE014134 AGB92700   AI108927   AI135058   AI237947   AI517193   AI517194   AI945474   AQ026055   AW940497   AX093882   AY060289 AAL25328   BE978602   BF503054   BI163125   BI167505   BI168232   BI168912   BI169305   BI213053   BI214918   BI229451   BI231286   BI232676   BI235526   BI236571   BI236731   BI237934   BI243117   BI361307   BI362592   BI362838   BI363069   BI363975   BI365276   BI365531   BI368231   BI369820   BI374639   BI374807   BI483985   BI573922   BI573923   BI577939   BI578253   BI578604   BI579210   BI579617   BI580351   BI580458   BI591411   BI591424   BI591808   BI606269   BI613705   BI621781   BI626686   BI627688   BI628734   BP558134   BP558135   BP559297   BT025048   CL527748   CO156501   CO188760   CO191353   CO196434   CO261947   CO264101   CO267593   CO267661   CO268462   CO268642   CO271401   CO271461   CO273432   CO274119   CO276141   CO277366   CO277459   CO285544   CO288351   CO290908   CO295693   CO297320   CO297786   CO299623   CO301960   CO310853   CO311412   CO312664   CO314128   CO315736   CO318702   CO319676   CO321354   CO321478   CO328943   CO330505   CO332793   CO339215   CZ473748   CZ475717   EC060515   EC061702   EC062537   EC064444   EC067668   EC067861   EC070761   EC072651   EC073448   EC075515   EC078951   EC081822   EC082238   EC083196   EC083396   EC087602   EC088246   EC089899   EC090507   EC195203   EC199231   EC202480   EC208203   EC209005   EC210979   EC215213   EC220442   EC220637   EC223092   EC223397   EC223467   EC224526   EC225338   EC226762   EC228621   EC231344   EC232101   EC233701   EC234785   EC243923   EC245989   EC248029   EC250187   EC250188   EC251702   EC251818   EC252163   EC258895   EL873200   EL873201   EL885356   EL885357   EV588947   GH749573   GH760259   GH785701   GH791304   GH801605   GH802705   GH804370   GH804899   GH805965   GH809631   GH816286   GH818900   GH821589   GH845213   GH850947   GH853516   GH855627   GH856224   GH864822   GH865009   GH866436   GH868937   GH872628   GH888516   GH892409   GH901101   GH906810   GH911710   GH914723   GH917979   GH921077   GH928894   GH938953   GH942137   GH946162   GH946182   GH946627   GH948658   GH949273   GH949598   GH950040   GH951279   GH951327   GH951696   GH952614   GH953224   GH954550   GH956352   GH956390   GH957496   GH960123   GH960606   GH961297   GH963329   GH964549   GH965744   GH967910   GH968628   GH974123   GH979802   GH979824   GH980604   GH980824   GH981510   GH981711   GH984925   U22439 AAC46609   U22440 AAC46610
UniProtKB/Swiss-Prot	Q24048
UniProtKB/TrEMBL	A4V0B5 Q1RL12
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References regulatory region FBsf0000435234 2L:6,796,263..6,805,006 evidence=Reporter construct (in vivo) (Xu et al., 1999, Halfon, 2012.6.19)
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) dorsal epidermis primordium   (Fisher et al., 2012) foregut primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain surface glia   (Fisher et al., 2012) embryonic dorsal epidermis   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) lateral cord surface glial cell   (Fisher et al., 2012) sensory nervous system primordium   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) RNase protection, primer extension, SI map Stage Tissue/Position (including subcellular localization) Reference adult stage   abdomen   (Sun et al., 1998)   (Sun et al., 1998) adult head   (Sun et al., 1998) adult abdomen   (Sun et al., 1998)
Additional Descriptive Data
	The 2.1kb isoform of nrv2 (Nrv2.2) is the major form expressed in fly heads. It is expressed at low levels in the abdomen. (Sun et al., 1998)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
dissected tissue Stage Tissue/Position (including subcellular localization) Reference adult stage   adult head   (Sun et al., 1998) adult thorax   (Sun et al., 1998) optic lobe   (Sun et al., 1998) thoracico-abdominal ganglion   (Sun et al., 1998) adult brain   (Sun et al., 1998) leg   (Sun et al., 1998) immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   longitudinal connective   (Sun and Salvaterra, 1995) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   central nervous system   (Sun and Salvaterra, 1995) commissure   (Sun and Salvaterra, 1995) embryonic/larval posterior spiracle   (Sun and Salvaterra, 1995) presumptive embryonic/larval peripheral nervous system   (Sun and Salvaterra, 1995) embryonic/larval garland cell   (Sun and Salvaterra, 1995) embryonic stage -- adult stage   head   (Sun and Salvaterra, 1995) adult stage   adult thorax   (Sun and Salvaterra, 1995) adult head   (Sun and Salvaterra, 1995) adult abdomen   (Sun and Salvaterra, 1995)
Additional Descriptive Data
	Nervana protein is detected in adult heads, thoraces and abdomens but is significantly enriched in heads on western blots. On developmental westerns, it is first observed in 6hr embryos and then throughout development. The levels of the different mobility components change through development. The 42K component is not observed until 12-24hr and is not prominent until the adult stage. Nervana protein is observed in the CNS and PNS by immunolocalization in a pattern indistinguishable from the anti-HRP pattern. Staining is also observed in the garland cells and the posterior spiracles. (Sun and Salvaterra, 1995) nrv2 is nervous system-specific. On western blots of dissected tissues, it is detected in adult heads and thorax, presumably in nervous system components. In adult tissue sections, it is detected predominantly in the nervous system, including the optic lobe, brain, thoracic ganglia, and axonal pathways in the leg. (Sun et al., 1998)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) septate junction inferred from direct assay (Genova and Fehon, 2003, Paul et al., 2007)
Expression Deduced from Reporters
Reporter: P{nrv2-GAL4.S} Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage   nervous system   (Sun et al., 1999) neuron   (Sun et al., 1999) larval stage -- adult stage   neuron | subset & glial cell | subset of central nervous system   (Sun et al., 2001) third instar larval stage   embryonic/larval wrapping glial cell   (Schmidt et al., 2012)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate or high levels. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system. Expression at moderate levels in the following post-embryonic organs or tissues: adult crop, larval/adult hindgut, adult heart, larval/adult salivary gland, larval trachea, adult spermathecae, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1528 Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head    991.1 Adult Eye    656.35 Adult Brain    1779.4 Adult Thoracic-Abdominal Ganglion    1860.5 Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1528) Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head  (991.1) Adult Eye  (656.35) Adult Brain  (1779.4) Adult Thoracic-Abdominal Ganglion  (1860.5) Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1528) Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head    991.1 Adult Eye    656.35 Adult Brain  (1779.4) Adult Thoracic-Abdominal Ganglion  (1860.5) Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1528 Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head    991.1 Adult Eye    656.35 Adult Brain    1779.4 Adult Thoracic-Abdominal Ganglion    1860.5 Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1528 Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head    991.1 Adult Eye    656.35 Adult Brain    1779.4 Adult Thoracic-Abdominal Ganglion    1860.5 Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1528) Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head  (991.1) Adult Eye  656.35 Adult Brain  (1779.4) Adult Thoracic-Abdominal Ganglion  (1860.5) Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1528 Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head    991.1 Adult Eye    656.35 Adult Brain  (1779.4) Adult Thoracic-Abdominal Ganglion  (1860.5) Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1528 Larval Midgut    27.5 Larval Hindgut    226.5 Larval Malpighian Tubules    14.8 Larval Fat Body    66.7 Larval Salivary Gland    213.7 Larval Trachea    464.85 Larval Carcass    145.1 Adult Head    991.1 Adult Eye    656.35 Adult Brain    1779.4 Adult Thoracic-Abdominal Ganglion    1860.5 Adult Crop    356.2 Adult Midgut    16.8 Adult Hindgut    122.1 Adult Malpighian Tubules    14.8 Adult Fat Body    68.1 Adult Salivary Gland    140.2 Adult Heart    264.1 Adult VirginFemale Spermatheca    122.9 Adult InseminatedFemale Spermatheca    142.9 Adult Ovary    30.4 Adult Testis    114 Adult Male Accessory Gland    170 Adult Carcass    236.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    991.1   NA  Eye    656.35   NA  Brain    1779.4   1528  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1860.5   NA  Crop    356.2   27.5  Midgut    16.8   226.5  Hindgut    122.1   no informative data  Malpighian Tubules    no informative data   66.7  Fat Body    68.1   213.7  Salivary Gland    140.2   NA  Heart    264.1   464.85  Trachea    NA   NA  VirginFemale Spermatheca    122.9   NA  InseminatedFemale Spermatheca    142.9   NA  Ovary    30.4   NA  Testis    114   NA  Male Accessory Gland    170   145.1  Carcass    236.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    87 central nervous system, larvae L3    54 central nervous system, pupae P8    103 head, virgin 1-day female    194 head, virgin 4-day female    93 head, virgin 20-day female    60 head, mated 1-day female    128 head, mated 4-day female    81 head, mated 20-day female    53 head, mated 1-day male    263 head, mated 4-day male    165 head, mated 20-day male    127 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8    86 carcass, larvae L3 wandering    28 carcass, 1-day adult    60 carcass, 4-day adult    44 carcass, 20-day adult    35 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    186 accessory gland, mated 4-day male    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (87) central nervous system, larvae L3  (54) central nervous system, pupae P8  (103) head, virgin 1-day female  (194) head, virgin 4-day female  (93) head, virgin 20-day female  (60) head, mated 1-day female  (128) head, mated 4-day female  (81) head, mated 20-day female  (53) head, mated 1-day male  (263) head, mated 4-day male  (165) head, mated 20-day male  (127) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8  (86) carcass, larvae L3 wandering  (28) carcass, 1-day adult  (60) carcass, 4-day adult  (44) carcass, 20-day adult  (35) ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male  (186) accessory gland, mated 4-day male  (31) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    87 central nervous system, larvae L3    54 central nervous system, pupae P8  103 head, virgin 1-day female  (194) head, virgin 4-day female    93 head, virgin 20-day female    60 head, mated 1-day female  (128) head, mated 4-day female    81 head, mated 20-day female    53 head, mated 1-day male  (263) head, mated 4-day male  (165) head, mated 20-day male  (127) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8    86 carcass, larvae L3 wandering    28 carcass, 1-day adult    60 carcass, 4-day adult    44 carcass, 20-day adult    35 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male  (186) accessory gland, mated 4-day male    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    87 central nervous system, larvae L3    54 central nervous system, pupae P8    103 head, virgin 1-day female    194 head, virgin 4-day female    93 head, virgin 20-day female    60 head, mated 1-day female    128 head, mated 4-day female    81 head, mated 20-day female    53 head, mated 1-day male    263 head, mated 4-day male    165 head, mated 20-day male    127 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8    86 carcass, larvae L3 wandering    28 carcass, 1-day adult    60 carcass, 4-day adult    44 carcass, 20-day adult    35 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    186 accessory gland, mated 4-day male    31 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    87 central nervous system, larvae L3    54 central nervous system, pupae P8    103 head, virgin 1-day female    194 head, virgin 4-day female    93 head, virgin 20-day female    60 head, mated 1-day female    128 head, mated 4-day female    81 head, mated 20-day female    53 head, mated 1-day male    263 head, mated 4-day male    165 head, mated 20-day male    127 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8    86 carcass, larvae L3 wandering    28 carcass, 1-day adult    60 carcass, 4-day adult    44 carcass, 20-day adult    35 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    186 accessory gland, mated 4-day male    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (87) central nervous system, larvae L3  (54) central nervous system, pupae P8  (103) head, virgin 1-day female  (194) head, virgin 4-day female  (93) head, virgin 20-day female  (60) head, mated 1-day female  (128) head, mated 4-day female  (81) head, mated 20-day female  (53) head, mated 1-day male  (263) head, mated 4-day male  (165) head, mated 20-day male  (127) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8  (86) carcass, larvae L3 wandering  28 carcass, 1-day adult  (60) carcass, 4-day adult  (44) carcass, 20-day adult  (35) ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male  (186) accessory gland, mated 4-day male  31 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    87 central nervous system, larvae L3    54 central nervous system, pupae P8  103 head, virgin 1-day female  (194) head, virgin 4-day female    93 head, virgin 20-day female    60 head, mated 1-day female  128 head, mated 4-day female    81 head, mated 20-day female    53 head, mated 1-day male  (263) head, mated 4-day male  (165) head, mated 20-day male  127 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8    86 carcass, larvae L3 wandering    28 carcass, 1-day adult    60 carcass, 4-day adult    44 carcass, 20-day adult    35 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male  (186) accessory gland, mated 4-day male    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    87 central nervous system, larvae L3    54 central nervous system, pupae P8    103 head, virgin 1-day female    194 head, virgin 4-day female    93 head, virgin 20-day female    60 head, mated 1-day female    128 head, mated 4-day female    81 head, mated 20-day female    53 head, mated 1-day male    263 head, mated 4-day male    165 head, mated 20-day male    127 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    11 digestive system, larvae L3 wandering    5 digestive system, 1-day adult    6 digestive system, 4-day adult    5 digestive system, 20-day adult    3 fat body, larvae L3 wandering    5 fat body, white prepupae    21 fat body, pupae P8    86 carcass, larvae L3 wandering    28 carcass, 1-day adult    60 carcass, 4-day adult    44 carcass, 20-day adult    35 ovary, virgin 4-day female    2 ovary, mated 4-day female    1 testis, mated 4-day male    186 accessory gland, mated 4-day male    31 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of very low expression. Peak expression observed within 06-24 hour embryonic stages, during early larval stages, at stages throughout the pupal period, in adult male stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr    35 embryo 06-08hr    79 embryo 08-10hr    87 embryo 10-12hr    110 embryo 12-14hr    96 embryo 14-16hr    62 embryo 16-18hr    80 embryo 18-20hr    92 embryo 20-22hr    68 embryo 22-24hr    93 larva L1    48 larva L2    28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    37 white prepupae 24hr    54 pupae 2d postWPP    68 pupae 3d postWPP    99 pupae 4d postWPP    80 adult male 01day    84 adult male 05day    78 adult male 30day    61 adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr  (35) embryo 06-08hr  (79) embryo 08-10hr  (87) embryo 10-12hr  (110) embryo 12-14hr  (96) embryo 14-16hr  (62) embryo 16-18hr  (80) embryo 18-20hr  (92) embryo 20-22hr  (68) embryo 22-24hr  (93) larva L1  (48) larva L2  (28) larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9  (28) white prepupae new  (29) white prepupae 12hr  (37) white prepupae 24hr  (54) pupae 2d postWPP  (68) pupae 3d postWPP  (99) pupae 4d postWPP  (80) adult male 01day  (84) adult male 05day  (78) adult male 30day  (61) adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr    35 embryo 06-08hr    79 embryo 08-10hr    87 embryo 10-12hr  (110) embryo 12-14hr    96 embryo 14-16hr    62 embryo 16-18hr    80 embryo 18-20hr    92 embryo 20-22hr    68 embryo 22-24hr    93 larva L1    48 larva L2    28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    37 white prepupae 24hr    54 pupae 2d postWPP    68 pupae 3d postWPP    99 pupae 4d postWPP    80 adult male 01day    84 adult male 05day    78 adult male 30day    61 adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr    35 embryo 06-08hr    79 embryo 08-10hr    87 embryo 10-12hr    110 embryo 12-14hr    96 embryo 14-16hr    62 embryo 16-18hr    80 embryo 18-20hr    92 embryo 20-22hr    68 embryo 22-24hr    93 larva L1    48 larva L2    28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    37 white prepupae 24hr    54 pupae 2d postWPP    68 pupae 3d postWPP    99 pupae 4d postWPP    80 adult male 01day    84 adult male 05day    78 adult male 30day    61 adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr    35 embryo 06-08hr    79 embryo 08-10hr    87 embryo 10-12hr    110 embryo 12-14hr    96 embryo 14-16hr    62 embryo 16-18hr    80 embryo 18-20hr    92 embryo 20-22hr    68 embryo 22-24hr    93 larva L1    48 larva L2    28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    37 white prepupae 24hr    54 pupae 2d postWPP    68 pupae 3d postWPP    99 pupae 4d postWPP    80 adult male 01day    84 adult male 05day    78 adult male 30day    61 adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr  (35) embryo 06-08hr  (79) embryo 08-10hr  (87) embryo 10-12hr  (110) embryo 12-14hr  (96) embryo 14-16hr  (62) embryo 16-18hr  (80) embryo 18-20hr  (92) embryo 20-22hr  (68) embryo 22-24hr  (93) larva L1  (48) larva L2  28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9  28 white prepupae new  29 white prepupae 12hr  (37) white prepupae 24hr  (54) pupae 2d postWPP  (68) pupae 3d postWPP  (99) pupae 4d postWPP  (80) adult male 01day  (84) adult male 05day  (78) adult male 30day  (61) adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr    35 embryo 06-08hr    79 embryo 08-10hr    87 embryo 10-12hr  110 embryo 12-14hr    96 embryo 14-16hr    62 embryo 16-18hr    80 embryo 18-20hr    92 embryo 20-22hr    68 embryo 22-24hr    93 larva L1    48 larva L2    28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    37 white prepupae 24hr    54 pupae 2d postWPP    68 pupae 3d postWPP    99 pupae 4d postWPP    80 adult male 01day    84 adult male 05day    78 adult male 30day    61 adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    8 embryo 04-06hr    35 embryo 06-08hr    79 embryo 08-10hr    87 embryo 10-12hr    110 embryo 12-14hr    96 embryo 14-16hr    62 embryo 16-18hr    80 embryo 18-20hr    92 embryo 20-22hr    68 embryo 22-24hr    93 larva L1    48 larva L2    28 larva L3 12hr old    15 larva L3 puffstage 1-2    16 larva L3 puffstage 3-6    23 larva L3 puffstage 7-9    28 white prepupae new    29 white prepupae 12hr    37 white prepupae 24hr    54 pupae 2d postWPP    68 pupae 3d postWPP    99 pupae 4d postWPP    80 adult male 01day    84 adult male 05day    78 adult male 30day    61 adult female 01day    24 adult female 05day    10 adult female 30day    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    35 embryonic 1182-4H    173 embryonic GM2    14 embryonic Kc167    26 embryonic S1    31 embryonic S3    3 leg disc CME L1    35 wing disc CME-W2    43 wing disc ML-DmD8    66 wing disc ML-DmD9    84 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    182 wing disc ML-DmD32    184 haltere disc ML-DmD17-c3    79 eye-antennal disc ML-DmD11    42 antennal disc ML-DmD20-c5    46 mixed discs ML-DmD4-c1    66 CNS ML-DmBG1-c1    53 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (27) Schneider line 2 Sg4  (35) embryonic 1182-4H  (173) embryonic GM2    14 embryonic Kc167  26 embryonic S1  (31) embryonic S3    3 leg disc CME L1  (35) wing disc CME-W2  (43) wing disc ML-DmD8  (66) wing disc ML-DmD9  (84) wing disc ML-DmD16-c3  (50) wing disc ML-DmD21  (182) wing disc ML-DmD32  (184) haltere disc ML-DmD17-c3  (79) eye-antennal disc ML-DmD11  (42) antennal disc ML-DmD20-c5  (46) mixed discs ML-DmD4-c1  (66) CNS ML-DmBG1-c1  (53) CNS ML-DmBG2-c2  (49) tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    35 embryonic 1182-4H  (173) embryonic GM2    14 embryonic Kc167    26 embryonic S1    31 embryonic S3    3 leg disc CME L1    35 wing disc CME-W2    43 wing disc ML-DmD8    66 wing disc ML-DmD9    84 wing disc ML-DmD16-c3    50 wing disc ML-DmD21  (182) wing disc ML-DmD32  (184) haltere disc ML-DmD17-c3    79 eye-antennal disc ML-DmD11    42 antennal disc ML-DmD20-c5    46 mixed discs ML-DmD4-c1    66 CNS ML-DmBG1-c1    53 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    35 embryonic 1182-4H    173 embryonic GM2    14 embryonic Kc167    26 embryonic S1    31 embryonic S3    3 leg disc CME L1    35 wing disc CME-W2    43 wing disc ML-DmD8    66 wing disc ML-DmD9    84 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    182 wing disc ML-DmD32    184 haltere disc ML-DmD17-c3    79 eye-antennal disc ML-DmD11    42 antennal disc ML-DmD20-c5    46 mixed discs ML-DmD4-c1    66 CNS ML-DmBG1-c1    53 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    35 embryonic 1182-4H    173 embryonic GM2    14 embryonic Kc167    26 embryonic S1    31 embryonic S3    3 leg disc CME L1    35 wing disc CME-W2    43 wing disc ML-DmD8    66 wing disc ML-DmD9    84 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    182 wing disc ML-DmD32    184 haltere disc ML-DmD17-c3    79 eye-antennal disc ML-DmD11    42 antennal disc ML-DmD20-c5    46 mixed discs ML-DmD4-c1    66 CNS ML-DmBG1-c1    53 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  27 Schneider line 2 Sg4  (35) embryonic 1182-4H  (173) embryonic GM2    14 embryonic Kc167  26 embryonic S1  31 embryonic S3    3 leg disc CME L1  (35) wing disc CME-W2  (43) wing disc ML-DmD8  (66) wing disc ML-DmD9  (84) wing disc ML-DmD16-c3  (50) wing disc ML-DmD21  (182) wing disc ML-DmD32  (184) haltere disc ML-DmD17-c3  (79) eye-antennal disc ML-DmD11  (42) antennal disc ML-DmD20-c5  (46) mixed discs ML-DmD4-c1  (66) CNS ML-DmBG1-c1  (53) CNS ML-DmBG2-c2  (49) tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    35 embryonic 1182-4H  (173) embryonic GM2    14 embryonic Kc167    26 embryonic S1    31 embryonic S3    3 leg disc CME L1    35 wing disc CME-W2    43 wing disc ML-DmD8    66 wing disc ML-DmD9    84 wing disc ML-DmD16-c3    50 wing disc ML-DmD21  (182) wing disc ML-DmD32  (184) haltere disc ML-DmD17-c3    79 eye-antennal disc ML-DmD11    42 antennal disc ML-DmD20-c5    46 mixed discs ML-DmD4-c1    66 CNS ML-DmBG1-c1    53 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    27 Schneider line 2 Sg4    35 embryonic 1182-4H    173 embryonic GM2    14 embryonic Kc167    26 embryonic S1    31 embryonic S3    3 leg disc CME L1    35 wing disc CME-W2    43 wing disc ML-DmD8    66 wing disc ML-DmD9    84 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    182 wing disc ML-DmD32    184 haltere disc ML-DmD17-c3    79 eye-antennal disc ML-DmD11    42 antennal disc ML-DmD20-c5    46 mixed discs ML-DmD4-c1    66 CNS ML-DmBG1-c1    53 CNS ML-DmBG2-c2    49 tumorous blood cells mbn2    20 ovary fGS/OSS    20 ovary OSC    16 ovary OSS    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    44 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult  (44) Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult  (43) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    44 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    44 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    44 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult  (44) Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult  (43) Paraquat 10 mM 48 hrs, 4-day adult  (44) Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    44 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    17 cold shock, 4-day adult    15 heat shock, 4-day adult    20 Cadmium 50 mM 6 hrs, larvae L3    17 Cadmium 50 mM 12 hrs, larvae L3    22 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    16 Copper 0.5 mM 12 hrs, larvae L3    13 Copper 15 mM 48 hrs, 4-day adult    19 Zinc 5 mM 12 hrs, larvae L3    10 Zinc 4.5 mM 48 hrs, 4-day adult    44 Ethanol 2.5% 3 hrs, larvae L3    23 Ethanol 5% 3 hrs, larvae L3    21 Ethanol 10% 3 hrs, larvae L3    17 Caffeine 1.5 mg/ml 4 hrs, larvae L3    17 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    18 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    44 Rotenone 2 μg 12 hrs, larvae L3    19 Rotenone 8 μg 12 hrs, larvae L3    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_24 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • GH13134 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015777 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9261-RC FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for nrv2 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive nrv2k04223 nrv2k13315 nrv2SH0329 viable, with Scer\GAL4pnr-MD237 nrv2GD960 Other Phenotypes Allele auditory perception defective, with Scer\GAL4nompA.cK nrv2GD960 neuroanatomy defective nrv2G74 visible, with Scer\GAL4pnr-MD237 nrv2GD960 Phenotype manifest in Allele dorsal trunk primordium nrv223B nrv2nwu3 embryonic/larval dorsal trunk nrv2k04223 embryonic/larval tracheal system nrv2k04223 embryonic/larval transverse connective nrv2k04223 embryonic ganglionic branch & embryonic tracheole nrv223B nrv2nwu3 embryonic somatic muscle nrv2unspecified ganglionic branch primordium nrv223B Johnston organ, with Scer\GAL4nompA.cK nrv2GD960 presumptive embryonic salivary gland nrv2unspecified septate junction nrv2k13315 nrv2unspecified septate junction & embryonic tracheal system nrv223B tracheal branch primordium nrv2unspecified trichogen cell, with Scer\GAL4pnr-MD237 nrv2GD960
Classical Alleles ( 16 )
For All Classical Alleles Show
Allele of nrv2 Class Mutagen Stocks Known lesion nrv2k13315 P-element activity 2 Yes nrv2ZCL1649 P-element activity 2 -- nrv2d04130 P-element activity 1 -- nrv2EY04390 P-element activity 1 -- nrv2k04223 loss of function allele P-element activity 1 -- nrv2MI03354 1 -- nrv211A P-element activity 0 Yes nrv223B loss of function allele P-element activity 0 Yes nrv2CPTI001455 piggyBac transposase 0 Yes nrv2d07415 P-element activity 0 -- nrv2G74 P-element activity 0 Yes nrv2nwu3 loss of function allele P-element activity 0 Yes nrv2nwu5 ethyl methanesulfonate 0 Yes nrv2nwu6 ethyl methanesulfonate 0 Yes nrv2SH0329 P-element activity 0 Yes nrv2unspecified 0 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of nrv2 Class Mutagen Stocks Known lesion nrv2GD960 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nrv2HMS01637 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nrv2JF03081 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes nrv21.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nrv21.Scer\UAS in vitro construct - regulatory fusion 0 Yes nrv22.Scer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes nrv22.Scer\UAS in vitro construct - regulatory fusion 0 Yes nrv2dsRNA.1.cPa in vitro construct - RNAi 0 Yes nrv2dsRNA.2.cPa in vitro construct - RNAi 0 Yes nrv2dsRNA.cPa in vitro construct - RNAi 0 Yes nrv2dsRNA.cRa in vitro construct - RNAi 0 Yes nrv2NIG.9261R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC7 (Gornek et al., 2001.10.2) Df(2L)Dwee1 (BDGP Project Members, 1994-1999) Df(2L)Dwee-δ5 (Spradling et al., 1999, Beitel and Krasnow, 2000, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2L)spdj2 (Bellen, 2000.8.28)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD960} NA P{NIG.9261R} NA P{TRiP.HMS01637} NA P{TRiP.JF03081} NA P{UAS-nrv2.1} NA P{UAS-Nrv2.2.Flag} NA P{UAS-nrv2.2} NA reporter construct P{nrv2-lacZ.7.7} No GAL4 construct P{nrv2-GAL4.S} No characterization construct P{UAS-nrv2::nrv3.Nrv2A} NA P{UAS-nrv2::nrv3.Nrv2AB} NA P{UAS-nrv2::nrv3.Nrv2ABC} NA P{UAS-nrv2::nrv3.Nrv2ABCD} NA P{UAS-nrv2::nrv3.Nrv2BCD} NA P{UAS-nrv2::nrv3.Nrv2CD} NA P{UAS-nrv2::nrv3.Nrv2D} NA P{UAS-Nrv2IT::Nrv3E} NA P{UAS-Nrv3I::Nrv2TE} NA P{UAS-Nrv3IT::Nrv2E} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}nrv2k04223 No P{lacW}nrv2k13315 No P{lacW}nrv2SH0329 No insertion of mobile activating element P{EPgy2}nrv2EY04390 NA P{XP}nrv2d04130 NA P{XP}nrv2d07415 NA miscellaneous insertions Mi{MIC}nrv2MI03354 NA P{PTT-GA}nrv2ZCL1649 NA P{PTT-un1}nrv2G74 NA PBac{681.P.FSVS-1}nrv2CPTI001455 NA
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 12 terms )
Molecular Function
CV term Evidence References
contributes_to cation transmembrane transporter activity inferred from direct assay (Sun et al., 1998) contributes_to sodium:potassium-exchanging ATPase activity inferred from mutant phenotype (Sun et al., 1998)
Biological Process
CV term Evidence References
cation transport inferred from direct assay (Sun et al., 1998) establishment of glial blood-brain barrier inferred from mutant phenotype (Stork et al., 2008, Schwabe et al., 2005) open tracheal system development inferred from mutant phenotype (Beitel and Krasnow, 2000) regulation of tube diameter, open tracheal system inferred from mutant phenotype (Paul et al., 2003) regulation of tube length, open tracheal system inferred from mutant phenotype (Paul et al., 2003) regulation of tube size, open tracheal system inferred from mutant phenotype (Beitel and Krasnow, 2000) sensory perception of sound inferred from mutant phenotype (Roy et al., 2013) septate junction assembly inferred from mutant phenotype (Paul et al., 2003)
Cellular Component
CV term Evidence References
plasma membrane inferred from direct assay (Khanna et al., 2010) septate junction inferred from direct assay (Genova and Fehon, 2003, Paul et al., 2007)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
sodium:potassium-exchanging ATPase activity inferred from sequence or structural similarity with UniProtKB:P25169 (FlyBase, 1992-) non-traceable author statement (Swiss-Prot Project Members, 2000.10.1)
Biological Process
CV term Evidence References
potassium ion transport inferred from electronic annotation with InterPro:IPR000402 (FlyBase Curators et al., 2004-) sodium ion transport inferred from electronic annotation with InterPro:IPR000402 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
plasma membrane inferred from sequence or structural similarity (Sun and Salvaterra, 1995) inferred from sequence or structural similarity with UniProtKB:P25169 (FlyBase, 1992-) sodium:potassium-exchanging ATPase complex inferred by curator from GO:0005391 (Sun et al., 1998) inferred from sequence or structural similarity with UniProtKB:P25169 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
nrv2 - Atpα anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) nrv2 - CG10581 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nrv2 - CG4872 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) nrv2 - CG7049 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nrv2 - Plod anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) nrv2 - Ugt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions nrv2 allele Gene References
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015777 DroID - A comprehensive database of gene and protein interactions. • FBgn0015777 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33953
Orthologs
OrthoDB Orthologs (48) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6N317W Dipteran inclusive ortholog search EOG6XPQQK Insect inclusive ortholog search EOG679D3P Arthropod inclusive ortholog search EOG62V6XT Metazoa inclusive ortholog search EOG6KWXMG
Orthologs in Drosophila Species (EOG6N317W)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\nrv2     Drosophila simulans   Dsim\GD22540 Y   Drosophila sechellia   Dsec\GM13825 Y   Drosophila erecta   Dere\GG23580 Y   Drosophila yakuba   Dyak\GE18402 Y   Drosophila ananassae   Dana\GF14479 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21652 Y   Drosophila persimilis   Dper\GL25960 Y   Drosophila willistoni   Dwil\GK24210 Y   Drosophila virilis   Dvir\GJ15658 Y   Drosophila mojavensis   Dmoj\GI17298 Y   Drosophila grimshawi   Dgri\GH10786 Y
Orthologs in non-Drosophila Dipterans (EOG6XPQQK)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL010145   Anopheles gambiae Malaria mosquito  Agam\AGAP007791   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801531
Orthologs in non-Dipteran Insects (EOG679D3P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC408722 Y Apis mellifera Western honey bee  Amel\GB46146 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006932 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG006935 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15064 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15065 Y Atta cephalotes Leafcutter ant  Acep\ACEP27139 Y Atta cephalotes Leafcutter ant  Acep\ACEP27142 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17317 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO17316 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14820 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14819 Y Linepithema humile Argentine ant  Lhum\LH16951 Y Linepithema humile Argentine ant  Lhum\LH16949 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17990 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB17987 Y Solenopsis invicta Red fire ant  Sinv\SINV23497 Y Solenopsis invicta Red fire ant  Sinv\SINV23539 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI009444 Y Bombyx mori Silkmoth  Bmor\BGIBMGA006099 Y Bombyx mori Silkmoth  Bmor\BGIBMGA006057 Y Bombyx mori Silkmoth  Bmor\BGIBMGA006062 Y Pediculus humanus Human body louse  Phum\PHUM583290 Y Pediculus humanus Human body louse  Phum\PHUM411340 Y Pediculus humanus Human body louse  Phum\PHUM465330 Y Tribolium castaneum Red flour beetle  Tcas\TC014030 Y Tribolium castaneum Red flour beetle  Tcas\TC014031 Y
Orthologs in non-Insect Arthropods (EOG62V6XT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\ND2 Y Ixodes scapularis Deer tick  Isca\ISCW016921 Y
Orthologs in non-Arthropod Metazoa (EOG6KWXMG)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\nkb-1 Y Caenorhabditis elegans Nematode  Cele\nkb-3 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001596gn Y Danio rerio Zebrafish  Drer\atp1b1a Y
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22540 Drosophila sechellia Dsec\GM13825 Drosophila erecta Dere\GG23580 Drosophila yakuba Dyak\GE18402 Drosophila ananassae Dana\GF14479 Drosophila pseudoobscura pseudoobscura Dpse\GA21652 Drosophila persimilis Dper\GL25960 Drosophila willistoni Dwil\GK24210 Drosophila virilis Dvir\GJ15658 Drosophila mojavensis Dmoj\GI17298 Drosophila grimshawi Dgri\GH10786
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	28666 y1 v1; P{TRiP.JF03081}attP2 41039 y1 w*; Mi{MIC}nrv2MI03354/SM6a 11086 y1 w67c23; P{lacW}nrv2k13315/CyO 6828 y1 w1118; P{PTT-GA}nrv2ZCL1649/CyO 16602 y1 w67c23; P{EPgy2}nrv2EY04390/CyO 37495 y1 sc* v1; P{TRiP.HMS01637}attP40/CyO
Harvard	- P{XP}nrv2d04130
Kyoto	110802 P{PTT-GA}nrv2ZCL1649 102305 y1 w67c23 ; P{lacW}nrv2k04223 / CyO 114351 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}nrv2k13315 P{neoFRT}40A/CyO y+
VDRC	v2660 w1118; P{GD960}v2660
Genomic Clones ( 1 )
	BACR22N03 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 169 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH13134 IP16413
Other clones	DPiM_GH13134
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT18959 GH07410 GH12674 GH27850 GH27851 LD13795 RE02450 RE07903 RE08825 RE09820 RE10442 RE18366 RE20734 RE21070 RE27232 RE28778 RE31381 RE32642 RE32838 RE34269 RE40674 RE45469 RE47193 RE47512 RE47787 RE48978 RE50532 RE50878 RE54163 RE56129 RE62278 RE62482 RE66981 RE71714 RE72104 RE72490 RE73204 RE73678 RE74534 RE74654 RH05181 RH05195 RH06790 RH42923 RH53185 RH57263 RH67692 RH69061 RH72521
Other clones	45631 61914 99658 bs13c10 bs80f11 CK01093 CK02385 Dmel_neb_008630_0089_0495 EC30744 EK004412 EK041805 EK068306 EK075531 EK082214 EK091526 EK092216 EK103328 EK103521 EK110934 EK113226 EK119801 EK123952 EK124728 EK137504 EK150948 EK151449 EK169226 EK170535 EK175950 EK182763 EK185517 EK185747 EK211348 EK216656 EK222825 EK224515 EK229617 EK235150 EK262336 EK273448 EK279941 EK291718 EK295430 EK304551 EN08207 EP01234 FGM313B10 FGM320A11 IP05036 IP16613 RH09380 RP003008152 RP003021437 RP003037783 RP003040858 RP003044958 RP003072534 RP003074771 RP003081223 RP003127648 RP003174920 RP003187417 RP003196457 RP003197151 RP003203578 RP003219492 RP003229925 RP003240010 RP003252718 RP003259332 RP003290472 RP003308335 RP003347637 RP003360972 RP003411368 RP003430641 RP003505252 RP003527586 RP003544874 RP003592022 RP003599693 RP003608182 RP003645683 RP003661351 RP004057688 RP004079784 RP004081048 RP004112728 RP004132841 RP004141996 RP004155131 RP004158123 RP004161675 RP004177521 RP004180546 RP004191022 RP004196957 RP004218057 RP004221878 RP004223427 RP004228740 RP004230522 RP004237344 RP004239754 RP004246458 RP004247194 RP004259517 RP004369612 RP004452517 RP004461799 RP004488192 RP004502532 RP004504169 RP004515559 RP004536169 RP004542878 RP004549048
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015777 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33953
Antibody Information
	monoclonal (Sun and Salvaterra, 1995) polyclonal (Sun et al., 1998)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ecta l(2)k04223 (Beitel and Krasnow, 2000) Source for identity of: Nrv2 CG9261 (Wortman, 1999.11)
Source for database merge of	Source for merge of: nrc2 ecta (Paul et al., 2003) Source for merge of: nrv2 l(2)SH0329 (FlyBase, 1992-) Source for merge of: nrv2 NEST:bs13c10 (Paul et al., 2003, FlyBase, 1996-)
Additional comments	Source for merge of nrv2 NEST:bs13c10 was sequence comparison (date:031021). (FlyBase, 1996-)
Other Comments
	1 allele of nrv2 recovered in a P-insertion screen. (Oh et al., 2003) Mutant embryos have lengthened primary tracheal tubes with expanded diameters, and defective septate junctions. (Paul et al., 2003) Area matching Drosophila Neuron surface antigen 2 gene, Acc. No. U22439. (Davies, 2001.3.15) Mutation in nrv2 alters tracheal tube size in embryos without affecting other aspects of tracheal development including primary branch budding and outgrowth or branch fusion. (Beitel and Krasnow, 2000) The product(s) of the Nervana gene(s) were recognised by their epitope(s) which reacts with anti-Horse Radish Peroxidase antibodies. These antibodies label neurons in Drosophila. The nac gene product may be required for the glycosylation of these proteins. (Sun and Salvaterra, 1995) Southern blot of cRNA probes hybridised to genomic DNA indicates nrv1 and nrv2 are derived from different genes. (Sun and Salvaterra, 1995) nrv1 and nrv2 are neuron-specific forms of the β subunit of Na+,K+-ATPase. (Sun and Salvaterra, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057820 NM_057821 NM_164711 NM_001014475 NM_001014476 NM_001273235 RefSeq (Proteins) • NP_477168 NP_477169 NP_723216 NP_001014475 NP_001014476 NP_001260164 DDBJ / EMBL / GenBank • AAC46609 AAC46610 AAF52438 AAF52438.2 AAF52439 AAF52439.1 AAL25328 AAN10600 AAN10600.1 AAX52659 AAX52660 AB081999 AC004303 AGB92700 AI237947 AI945474 AQ026055 AW940497 AX093882 AY060289 CL527748 CZ473748 CZ475717 U22439 U22440 Entrez Gene - A searchable database of RefSeq genes. • 33953 UniProtKB/Swiss-Prot • Q24048 UniProtKB/TrEMBL • A4V0B5 Q1RL12
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sodium/potassium-transporting ATPase subunit beta (IPR000402) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH13134
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015777 DroID - A comprehensive database of gene and protein interactions. • FBgn0015777 DRSC - Results from RNAi screens. • FBgn0015777 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015777 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9261-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015777 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33953 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33953 modMine - Data generated by the modENCODE project. • FBgn0015777
Synonyms & Secondary IDs ( 29 )
Reported As
Symbol Synonym	42kDa (Pahud et al., 1998) CG9261 (Rendic et al., 2006, Quinones-Coello, 2007, Schiess et al., 2009, Ni et al., 2011.7.19, Perkins et al., 2009, Khanna et al., 2010, Ling and Salvaterra, 2011, Cammarato et al., 2011) ecta   l(2)k04223 (Spradling et al., 1999, ) l(2)k13315 (Spradling et al., 1999, Kaufmann et al., 2002, ) l(2)SH2 0329 (Oh et al., 2002.3.22) l(2)SH0329   Na,K-ATPase (Okamura et al., 2001) Na+/K+ ATPase (Chen and Haddad, 2004) Na+/K+ATPase (Chen et al., 2002) Na+/K+-ATPase (Linton and O'Donnell, 1999) NaK ATPase (Wu and Beitel, 2003) NEST:bs13c10   Nrv   Nrv2 (Cooley et al., 2002.10-2003.5, Bellen, 2000.8.28, Emery et al., 1998, Prokop, 1999, Sun et al., 1998, Altenhein et al., 2006, Paul et al., 2007, Wang et al., 2004, Górska-Andrzejak et al., 2009, Larsen et al., 2009, Fung et al., 2008, Oland et al., 2008) nrv2 (Schwabe et al., 2005, Stork et al., 2008, Paul et al., 2007, Quinones-Coello, 2007, Wu et al., 2007, Dietzl et al., 2007, Baer et al., 2007, Guan et al., 2011, Baumann et al., 2010, Xie and Auld, 2011, Cash and Andrews, 2012, Yi et al., 2008, Stork et al., 2009, Spindler et al., 2009, Tolhuis et al., 2006, Younossi-Hartenstein et al., 2006, Quijano et al., 2008, Nilton et al., 2010, Li et al., 2007, Ling and Salvaterra, 2011, Roy et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Nelson et al., 2012, Shirasaki et al., 2012) nrv2.2 (Rendic et al., 2006) Nrv2.2 (Gorokhova et al., 2007) unnamed (Snow et al., 1987)
Name Synonym	ectatic (Beitel and Krasnow, 2000) Na+/K+ ATPase (Nilton et al., 2010) Na+/K+ ATPase (Ling and Salvaterra, 2011) Nervana (Genova and Fehon, 2001, Rendic et al., 2006, Beramendi et al., 2007) nervana (Sun and Salvaterra, 1995) nervana2 (Morin et al., 2001, Yi et al., 2008, Stork et al., 2008, Nair et al., 2007) Nervana 2 (Tan et al., 2009, Xie and Auld, 2011, Guan et al., 2011, Górska-Andrzejak et al., 2009, Khanna et al., 2010) nervana 2 (Baer et al., 2007, Cash and Andrews, 2012, Nilton et al., 2010, Cammarato et al., 2011) Nervana2 (Roy et al., 2007, Oland et al., 2008) Nirvana   nirvana2 (Spindler et al., 2009)
Secondary FlyBase IDs
	FBgn0014867 FBgn0021885 FBgn0022206 FBgn0045319 FBgn0065838
References ( 116 )
Generate a list of	All references relating to this gene ( 116 )
List References by type	Research paper  ( 58 ) Supplementary material  ( 9 ) Review  ( 4 ) Personal communication to FlyBase  ( 19 ) Abstract  ( 15 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Patent  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Letter  ( 1 )
Recent research papers ( 10 )
	Roy et al., 2013, Proc. Natl. Acad. Sci. U.S.A. 110(1): 181--186 Cell-type-specific roles of Na+/K+ ATPase subunits in Drosophila auditory mechanosensation. [FBrf0220434] Cash and Andrews, 2012, BMC Dev. Biol. 12: 4 Fine scale analysis of gene expression in Drosophila melanogaster gonads reveals Programmed cell death 4 promotes the differentiation of female germline stem cells. [FBrf0217547] Nelson et al., 2012, Nat. Cell Biol. 14(5): 518--525 Drosophila Src regulates anisotropic apical surface growth to control epithelial tube size. [FBrf0218197] Schmidt et al., 2012, J. Neurosci. 32(22): 7466--7476 Kinesin heavy chain function in Drosophila glial cells controls neuronal activity. [FBrf0218491] Shirasaki et al., 2012, Neuron 75(1): 41--57 Network organization of the huntingtin proteomic interactome in Mammalian brain. [FBrf0218881] Baycin-Hizal et al., 2011, J. Proteome Res. 10(6): 2777--2784 GlycoFly: A Database of Drosophila N-linked Glycoproteins Identified Using SPEG-MS Techniques. [FBrf0213834] Guan et al., 2011, Learn. Mem. 18(4): 191--206 Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants. [FBrf0213277] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Oshima and Fehon, 2011, J. Cell Sci. 124(16): 2861--2871 Analysis of protein dynamics within the septate junction reveals a highly stable core protein complex that does not include the basolateral polarity protein Discs large. [FBrf0214577] Xie and Auld, 2011, Development 138(17): 3813--3822 Integrins are necessary for the development and maintenance of the glial layers in the Drosophila peripheral nerve. [FBrf0214589] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...