Gene Dmel\Rab14

(EDGP Project Members, 1994-)

35A3-35A4

(determined by in situ hybridisation)

(Spradling et al., 1999)

34F-35A

(determined by in situ hybridisation)

(Louis et al., 1997)

34F-35A

Cytological site tentative; in situ of cosmid 57H4 gives repetitive signals. (determined by in situ hybridisation)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Complementation data suggests that l(2)34Fd is distal to Rab14.

(Ashburner et al., 1999.10.12)

Excisions of Rab14^k08712 indicate that Rab14 is between l(2)34Fd and Pgant35A.

Gene Model & Products

Please see the GBrowse view of Dmel\Rab14 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.40

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Rab14-RA

FBtr0080626

NM_057823

1145

215

Rab14-RB

FBtr0080627

NM_176042

1468

239

Rab14-RC

FBtr0080628

NM_176043

878

233

Additional Transcript Data & Comments

Reported size (kB)

1.116 (longest cDNA)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Rab14-PA

FBpp0080199

24.3

215

6.13

NP_477171

AAF53390

Rab14-PB

FBpp0080200

27.1

239

7.02

NP_788056

AAO41193

Rab14-PC

FBpp0080201

26.3

233

6.13

NP_788057

AAO41194

Additional Polypeptide Data & Comments

Reported size (kDa)

215 (aa)

Comments

External Data

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Small GTPase superfamily (IPR001806)

Small GTPase superfamily, Rab type (IPR003579)

Small GTP-binding protein domain (IPR005225)

P-loop containing nucleoside triphosphate hydrolase (IPR027417)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Crossreferences

Expression Data

Transcript Expression

expression microarray

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

eye

(Xu et al., 2004)

Additional Descriptive Data

Eye-enriched transcripts determined by ratio of expression level in wild-type heads. versus expression level in so heads.

(Xu et al., 2004)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

(The modENCODE Consortium, 2010, Graveley et al., 2011)

References

neuronal cell body

inferred from direct assay

(Chan et al., 2011, Jin et al., 2012)

synapse

inferred from direct assay

(Jin et al., 2012)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval Malpighian tubules, adult heart, larval salivary gland, adult testis. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, adult Malpighian tubules, larval/adult fat body, adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		1153.95
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		(693.4)
Larval Fat Body		302.3
Larval Salivary Gland		(629.6)
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		(586.7)
Adult Eye		(1153.95)
Adult Brain		(793.6)
Adult Thoracic-Abdominal Ganglion		(799.1)
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		(743.7)
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		(689.1)
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		(1153.95)
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		1153.95
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		1153.95
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		(1153.95)
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		1153.95
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		353.425
Larval Midgut		240.2
Larval Hindgut		307.1
Larval Malpighian Tubules		693.4
Larval Fat Body		302.3
Larval Salivary Gland		629.6
Larval Trachea		252.275
Larval Carcass		199.8
Adult Head		586.7
Adult Eye		1153.95
Adult Brain		793.6
Adult Thoracic-Abdominal Ganglion		799.1
Adult Crop		438.3
Adult Midgut		234.9
Adult Hindgut		399.1
Adult Malpighian Tubules		443
Adult Fat Body		311.4
Adult Salivary Gland		320.6
Adult Heart		743.7
Adult VirginFemale Spermatheca		283.2
Adult InseminatedFemale Spermatheca		298.3
Adult Ovary		367.1
Adult Testis		689.1
Adult Male Accessory Gland		316.7
Adult Carcass		358.1
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	586.7
NA	Eye	1153.95
NA	Brain	793.6
353.425	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	799.1
NA	Crop	438.3
240.2	Midgut	234.9
307.1	Hindgut	399.1
693.4	Malpighian Tubules	443
302.3	Fat Body	311.4
629.6	Salivary Gland	320.6
NA	Heart	743.7
252.275	Trachea	NA
NA	VirginFemale Spermatheca	283.2
NA	InseminatedFemale Spermatheca	298.3
NA	Ovary	367.1
NA	Testis	689.1
NA	Male Accessory Gland	316.7
199.8	Carcass	358.1

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		45
head, mated 4-day male		42
head, mated 20-day male		47
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		51
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		42
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		(28)
head, virgin 1-day female		(28)
head, virgin 4-day female		(30)
head, virgin 20-day female		26
head, mated 1-day female		(27)
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		(45)
head, mated 4-day male		(42)
head, mated 20-day male		(47)
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		(51)
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		(28)
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		(32)
ovary, mated 4-day female		(32)
testis, mated 4-day male		(42)
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		45
head, mated 4-day male		42
head, mated 20-day male		47
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		51
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		42
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		45
head, mated 4-day male		42
head, mated 20-day male		47
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		51
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		42
accessory gland, mated 4-day male		12
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		45
head, mated 4-day male		42
head, mated 20-day male		47
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		51
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		42
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		(45)
head, mated 4-day male		(42)
head, mated 20-day male		(47)
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		(51)
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		(42)
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		45
head, mated 4-day male		42
head, mated 20-day male		47
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		51
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		42
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		22
central nervous system, larvae L3		19
central nervous system, pupae P8		28
head, virgin 1-day female		28
head, virgin 4-day female		30
head, virgin 20-day female		26
head, mated 1-day female		27
head, mated 4-day female		24
head, mated 20-day female		19
head, mated 1-day male		45
head, mated 4-day male		42
head, mated 20-day male		47
salivary gland, larvae L3 wandering		22
salivary gland, white prepupae		25
digestive system, larvae L3 wandering		51
digestive system, 1-day adult		11
digestive system, 4-day adult		12
digestive system, 20-day adult		13
fat body, larvae L3 wandering		6
fat body, white prepupae		17
fat body, pupae P8		28
carcass, larvae L3 wandering		19
carcass, 1-day adult		15
carcass, 4-day adult		15
carcass, 20-day adult		19
ovary, virgin 4-day female		32
ovary, mated 4-day female		32
testis, mated 4-day male		42
accessory gland, mated 4-day male		12
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed in adult male stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		39
embryo 02-04hr		37
embryo 04-06hr		38
embryo 06-08hr		35
embryo 08-10hr		39
embryo 10-12hr		36
embryo 12-14hr		33
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		41
white prepupae new		41
white prepupae 12hr		35
white prepupae 24hr		30
pupae 2d postWPP		42
pupae 3d postWPP		35
pupae 4d postWPP		25
adult male 01day		42
adult male 05day		48
adult male 30day		45
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(39)
embryo 02-04hr		(37)
embryo 04-06hr		(38)
embryo 06-08hr		(35)
embryo 08-10hr		(39)
embryo 10-12hr		(36)
embryo 12-14hr		(33)
embryo 14-16hr		(31)
embryo 16-18hr		(31)
embryo 18-20hr		(29)
embryo 20-22hr		24
embryo 22-24hr		(31)
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		(27)
larva L3 puffstage 7-9		(41)
white prepupae new		(41)
white prepupae 12hr		(35)
white prepupae 24hr		(30)
pupae 2d postWPP		(42)
pupae 3d postWPP		(35)
pupae 4d postWPP		25
adult male 01day		(42)
adult male 05day		(48)
adult male 30day		(45)
adult female 01day		(28)
adult female 05day		(31)
adult female 30day		(30)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		39
embryo 02-04hr		37
embryo 04-06hr		38
embryo 06-08hr		35
embryo 08-10hr		39
embryo 10-12hr		36
embryo 12-14hr		33
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		41
white prepupae new		41
white prepupae 12hr		35
white prepupae 24hr		30
pupae 2d postWPP		42
pupae 3d postWPP		35
pupae 4d postWPP		25
adult male 01day		42
adult male 05day		48
adult male 30day		45
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		39
embryo 02-04hr		37
embryo 04-06hr		38
embryo 06-08hr		35
embryo 08-10hr		39
embryo 10-12hr		36
embryo 12-14hr		33
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		41
white prepupae new		41
white prepupae 12hr		35
white prepupae 24hr		30
pupae 2d postWPP		42
pupae 3d postWPP		35
pupae 4d postWPP		25
adult male 01day		42
adult male 05day		48
adult male 30day		45
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		39
embryo 02-04hr		37
embryo 04-06hr		38
embryo 06-08hr		35
embryo 08-10hr		39
embryo 10-12hr		36
embryo 12-14hr		33
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		41
white prepupae new		41
white prepupae 12hr		35
white prepupae 24hr		30
pupae 2d postWPP		42
pupae 3d postWPP		35
pupae 4d postWPP		25
adult male 01day		42
adult male 05day		48
adult male 30day		45
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(39)
embryo 02-04hr		(37)
embryo 04-06hr		(38)
embryo 06-08hr		(35)
embryo 08-10hr		(39)
embryo 10-12hr		(36)
embryo 12-14hr		(33)
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		(41)
white prepupae new		(41)
white prepupae 12hr		(35)
white prepupae 24hr		30
pupae 2d postWPP		(42)
pupae 3d postWPP		(35)
pupae 4d postWPP		25
adult male 01day		(42)
adult male 05day		(48)
adult male 30day		(45)
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		39
embryo 02-04hr		37
embryo 04-06hr		38
embryo 06-08hr		35
embryo 08-10hr		39
embryo 10-12hr		36
embryo 12-14hr		33
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		41
white prepupae new		41
white prepupae 12hr		35
white prepupae 24hr		30
pupae 2d postWPP		42
pupae 3d postWPP		35
pupae 4d postWPP		25
adult male 01day		42
adult male 05day		48
adult male 30day		45
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		39
embryo 02-04hr		37
embryo 04-06hr		38
embryo 06-08hr		35
embryo 08-10hr		39
embryo 10-12hr		36
embryo 12-14hr		33
embryo 14-16hr		31
embryo 16-18hr		31
embryo 18-20hr		29
embryo 20-22hr		24
embryo 22-24hr		31
larva L1		18
larva L2		14
larva L3 12hr old		10
larva L3 puffstage 1-2		18
larva L3 puffstage 3-6		27
larva L3 puffstage 7-9		41
white prepupae new		41
white prepupae 12hr		35
white prepupae 24hr		30
pupae 2d postWPP		42
pupae 3d postWPP		35
pupae 4d postWPP		25
adult male 01day		42
adult male 05day		48
adult male 30day		45
adult female 01day		28
adult female 05day		31
adult female 30day		30
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		42
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		42
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		37
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		(32)
embryonic GM2		26
embryonic Kc167		(32)
embryonic S1		(42)
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		(42)
wing disc ML-DmD21		(27)
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		(38)
tumorous blood cells mbn2		(37)
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		42
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		42
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		37
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		42
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		42
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		37
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		42
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		42
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		37
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		(42)
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		(42)
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		(38)
tumorous blood cells mbn2		(37)
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		42
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		42
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		37
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		24
Schneider line 2 Sg4		11
embryonic 1182-4H		32
embryonic GM2		26
embryonic Kc167		32
embryonic S1		42
embryonic S3		24
leg disc CME L1		15
wing disc CME-W2		12
wing disc ML-DmD8		15
wing disc ML-DmD9		24
wing disc ML-DmD16-c3		42
wing disc ML-DmD21		27
wing disc ML-DmD32		19
haltere disc ML-DmD17-c3		19
eye-antennal disc ML-DmD11		20
antennal disc ML-DmD20-c5		25
mixed discs ML-DmD4-c1		25
CNS ML-DmBG1-c1		23
CNS ML-DmBG2-c2		38
tumorous blood cells mbn2		37
ovary fGS/OSS		20
ovary OSC		22
ovary OSS		14
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		(27)
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		(30)
Paraquat 10 mM 48 hrs, 4-day adult		(29)
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		13
cold shock, 4-day adult		10
heat shock, 4-day adult		16
Cadmium 50 mM 6 hrs, larvae L3		9
Cadmium 50 mM 12 hrs, larvae L3		21
Cadmium 50 mM 48 hrs, 4-day adult		16
Cadmium 100 mM 48 hrs, 4-day adult		27
Copper 0.5 mM 12 hrs, larvae L3		6
Copper 15 mM 48 hrs, 4-day adult		23
Zinc 5 mM 12 hrs, larvae L3		11
Zinc 4.5 mM 48 hrs, 4-day adult		23
Ethanol 2.5% 3 hrs, larvae L3		25
Ethanol 5% 3 hrs, larvae L3		17
Ethanol 10% 3 hrs, larvae L3		11
Caffeine 1.5 mg/ml 4 hrs, larvae L3		16
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		26
Caffeine 25 mg/ml 48 hrs, 4-day adult		13
Paraquat 5 mM 48 hrs, 4-day adult		30
Paraquat 10 mM 48 hrs, 4-day adult		29
Rotenone 2 μg 12 hrs, larvae L3		11
Rotenone 8 μg 12 hrs, larvae L3		10
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

External Data & Images

PBac{I-SceI(FRT.Rab14-GAL4.ATG(loxP.3xP3-RFP))}

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015791

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4212-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

viable

Rab14^EY04032

viable, with Scer\GAL4^pnr-MD237

Rab14^GD10513

Sterility

Allele

fertile

Rab14^EY04032

Other Phenotypes

Allele

wild-type

Rab14^k08712

Phenotype manifest in

Allele

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of Rab14	Mutagen	Stocks	Known lesion
Rab14^BG01134	P-element activity	1	--
Rab14^EY04032	P-element activity	1	--
Rab14^k08712	P-element activity	1	Yes
Rab14^DG17403		0	--

Alleles Carried on Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Rab14	Mutagen	Stocks	Known lesion
Rab14^{Q94L.Scer\UAS.P\T.T:Avic\GFP-YFP}	in vitro construct - amino acid replacement in vitro construct - regulatory fusion in vitro construct - coding region fusion	2	Yes
Rab14^{S49N.Scer\UAS.P\T.T:Avic\GFP-YFP}	in vitro construct - amino acid replacement in vitro construct - regulatory fusion in vitro construct - coding region fusion	2	Yes
Rab14^{Scer\UAS.P\T.T:Avic\GFP-YFP}	in vitro construct - regulatory fusion in vitro construct - coding region fusion	2	Yes
Rab14^GD10513	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Rab14^HMS01130	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Rab14^JF03135	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Rab14^KK108160	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
Rab14^NIG.4212R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2L)Exel6035

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{UASp-YFP.Rab14.Q94L}

P{UASp-YFP.Rab14.S49N}

P{UASp-YFP.Rab14}

GAL4 construct

Insertions

Type of insertions

Name

Expression data

miscellaneous insertions

P{GT1}Rab14^BG01134

P{wHy}Rab14^DG17403

insertion of enhancer trap

P{lacW}Rab14^k08712

insertion of mobile activating element

P{EPgy2}Rab14^EY04032

Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function ( 0 terms)

Biological Process ( 0 terms)

Cellular Component

CV term

References

neuronal cell body

inferred from direct assay

(Chan et al., 2011, Jin et al., 2012)

synapse

inferred from direct assay

(Jin et al., 2012)

Terms Based on Predictions or Assertions ( 5 terms )

Molecular Function

CV term

(Kohno et al., 1994, Ashburner et al., 1999)

References

GTPase activity

inferred from sequence or structural similarity

inferred from sequence or structural similarity with UniProtKB:P35287

(FlyBase, 1992-)

GTP binding

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579, InterPro:IPR005225

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(EDGP Project Members, 1994-)

Biological Process

CV term

(FlyBase Curators et al., 2004-)

References

protein transport

inferred from electronic annotation with InterPro:IPR003579

small GTPase mediated signal transduction

inferred from electronic annotation with InterPro:IPR001806, InterPro:IPR003579

(FlyBase Curators et al., 2004-)

Cellular Component

CV term

References

vesicle

inferred from sequence or structural similarity with Rab11

(Curators, 2008--)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

Rab14 - CG32219

experimental knowledge based

(Guruharsha et al., 2011, Mintseris et al., 2009.11.23)

Rab14 - E(spl)m8-HLH

experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting

Rab14 - Fbp2

experimental knowledge based

(Mintseris et al., 2009.11.23)

Rab14 - Gdi

anti tag coimmunoprecipitation, peptide massfingerprinting

Rab14 - Rab7

experimental knowledge based

Rab14 - RpS15Ab

experimental knowledge based

(Mintseris et al., 2009.11.23)

Rab14 - Vha36-1

anti tag coimmunoprecipitation, peptide massfingerprinting

Rab14 - Vps4

anti tag coimmunoprecipitation, peptide massfingerprinting

(Mintseris et al., 2009.11.23)

Rab14 - gsb

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Mintseris et al., 2009.11.23, Guruharsha et al., 2011)

Rab14 - βTub85D

experimental knowledge based

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Rab14 allele

Gene

References

External Data

y¹ v¹; P{TRiP.JF03135}attP2

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0015791

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0015791

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

34840

Orthologs

OrthoDB Orthologs (67) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG6D2796

Dipteran inclusive ortholog search

EOG6H1B4S

Insect inclusive ortholog search

EOG6VDNVM

Arthropod inclusive ortholog search

EOG65MKNC

Metazoa inclusive ortholog search

EOG6NPKXZ

Orthologs in Drosophila Species (EOG6D2796)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18036

Drosophila persimilis

Dper\GL21269

Drosophila willistoni

Dwil\GK18273

Drosophila virilis

Dvir\GJ18183

Drosophila mojavensis

Dmoj\GI12880

Drosophila grimshawi

Dgri\GH11344

Orthologs in non-Drosophila Dipterans (EOG6H1B4S)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013252

Anopheles gambiae

Malaria mosquito

Agam\AGAP008419

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ004858

Orthologs in non-Dipteran Insects (EOG6VDNVM)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\Rab14

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG008053

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH12776

Atta cephalotes

Leafcutter ant

Acep\ACEP22099

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO15750

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL18379

Linepithema humile

Argentine ant

Lhum\LH20371

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB11977

Solenopsis invicta

Red fire ant

Sinv\SINV21547

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000787

Bombyx mori

Silkmoth

Bmor\BGIBMGA006221

Pediculus humanus

Human body louse

Phum\PHUM137540

Tribolium castaneum

Red flour beetle

Tcas\TC013891

Orthologs in non-Insect Arthropods (EOG65MKNC)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\cscB

Daphnia pulex

Water flea

Dpux\JGI_V11_304738

Ixodes scapularis

Deer tick

Isca\ISCW018962

Ixodes scapularis

Deer tick

Isca\ISCW013805

Orthologs in non-Arthropod Metazoa (EOG6NPKXZ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\unc-108

Caenorhabditis elegans

Nematode

Cele\F11A5.3

Caenorhabditis elegans

Nematode

Cele\F11A5.4

Caenorhabditis elegans

Nematode

Cele\rab-14

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_014913gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016605gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_019458gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_011157gn

Danio rerio

Zebrafish

Drer\zgc:101015

Danio rerio

Zebrafish

Drer\rab14

Danio rerio

Zebrafish

Drer\rab14l

Danio rerio

Zebrafish

Drer\rab2a

Danio rerio

Zebrafish

Drer\rab14

Danio rerio

Zebrafish

Drer\rab4a

Xenopus tropicalis

Western clawed frog

Xtro\rab4b

Xenopus tropicalis

Western clawed frog

Xtro\rab2a

Xenopus tropicalis

Western clawed frog

Xtro\rab14

Xenopus tropicalis

Western clawed frog

Xtro\rab2b

Gallus gallus

Domestic chicken

Ggal\RAB2A

Gallus gallus

Domestic chicken

Ggal\RAB4A

Gallus gallus

Domestic chicken

Ggal\RAB14

Mus musculus

House mouse

Mmus\Rab4b

Mus musculus

House mouse

Mmus\Rab2a

Mus musculus

House mouse

Mmus\Rab4a

Mus musculus

House mouse

Mmus\Rab14

Mus musculus

House mouse

Mmus\Rab2b

Rattus norvegicus

Norway rat

Rnor\Rab2b

Rattus norvegicus

Norway rat

Rnor\Rab2a

Rattus norvegicus

Norway rat

Rnor\Rab4a

Rattus norvegicus

Norway rat

Rnor\Rab14

Rattus norvegicus

Norway rat

Rnor\Rab4b

Homo sapiens

Human

Hsap\RAB4B

Homo sapiens

Human

Hsap\RAB2A

Homo sapiens

Human

Hsap\RAB2B

Homo sapiens

Human

Hsap\RAB14

Homo sapiens

Human

Hsap\ENSG00000168118

Human Orthologs (5)

Gene

OMIM

HGNC

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA18036

Drosophila persimilis

Dper\GL21269

Drosophila willistoni

Dwil\GK18273

Drosophila virilis

Dvir\GJ18183

Drosophila mojavensis

Dmoj\GI12880

Drosophila grimshawi

Dgri\GH11344

Stocks & Reagents

Stocks Listed in FlyBase ( 13 )

Bloomington

12457

w¹¹¹⁸; P{GT1}Rab14^BG01134

28708

16586

y¹ w^67c23; P{EPgy2}Rab14^EY04032

9793

y¹ w^*; P{UASp-YFP.Rab14}CG9967^5L

34654

y¹ sc^* v¹; P{TRiP.HMS01130}attP2

9795

y¹ w^*; P{UASp-YFP.Rab14.Q94L}Sos⁰²

9794

y¹ w^*; P{UASp-YFP.Rab14}12/TM3, Sb¹

23262

y¹ w^*; P{UASp-YFP.Rab14.Q94L}CG42673⁰⁷

23263

y¹ w^*; P{UASp-YFP.Rab14.S49N}Cas⁰¹/CyO

Kyoto

102662

y¹ w^67c23 ; P{lacW}Rab14^k08712 / CyO

VDRC

v104392

P{KK108160}VIE-260B

v45774

w¹¹¹⁸; P{GD10513}v45774

More stocks available...

Genomic Clones ( 1 )

BACR37K07

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 68 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

DRSC - Results from RNAi screens.

•

FBgn0015791

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

34840

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: Rab14 CG4212

(Kodira, 1999.11)

Source for database merge of

Source for merge of: Rab14 ESTS:57H4T

Additional comments

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Kouranti et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Data suggests that Rab14 is not a vital gene.

Isolated from a Drosophila head cDNA library.

(Ashburner et al., 1999.10.12, Ashburner et al., 1999)

This gene was first recognised by a match of an STS sequence from a European Drosophila Genome Mapping Project cosmid.

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

RefSeq (Proteins)

•

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

34840

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Small GTPase superfamily (IPR001806)

Small GTPase superfamily, Rab type (IPR003579)

Small GTP-binding protein domain (IPR005225)

P-loop containing nucleoside triphosphate hydrolase (IPR027417)

Linkouts

DPiM - DPiM, Drosophila Protein interaction Map

•

FBgn0015791

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0015791

DRSC - Results from RNAi screens.

•

FBgn0015791

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0015791

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG4212-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0015791

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

34840

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

34840

modMine - Data generated by the modENCODE project.

•

FBgn0015791

Synonyms & Secondary IDs ( 14 )

Reported As

Symbol Synonym

57H4T

(Louis et al., 1997)

AAF53390

(Armstrong, 2000)

anon-sts33

BG:DS01068.7

CG4212

(Verstreken and Bellen, 2000.9.4, Moorman et al., 2006, Zhang et al., 2007, Sinka et al., 2008, Keleman et al., 2009.8.5, Perkins et al., 2009, Ni et al., 2010.12.1, Colicelli, 2004)

DmRab14

(Pereira-Leal and Seabra, 2001, Pereira-Leal and Seabra, 2001)

DRab14