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General Information
Symbol
Dmel\Rbf
Species
D. melanogaster
Name
Retinoblastoma-family protein
Annotation Symbol
CG7413
Feature Type
FlyBase ID
FBgn0015799
Gene Model Status
Stock Availability
Gene Snapshot
Retinoblastoma-family protein (Rbf) encodes a protein that binds to members of the E2F transcription factors and forms the DREAM complex, which regulates genes with diverse biological functions including cell cycle regulators, apoptosis regulators, and DNA replication/repair factors. The product of Rbf can also bind chromosomal proteins and modulate genome stability. It is negatively regulated by CycE-Cdk2 and CycD-Cdk4 cyclin-dependent kinase complexes. [Date last reviewed: 2019-03-14]
Also Known As

Rbf1, Rb, EG:34F3.3 , pRb, Retinoblastoma

Key Links
Genomic Location
Cytogenetic map
Sequence location
X:797,090..801,479 [-]
Recombination map

1-0

RefSeq locus
NC_004354 REGION:797090..801479
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (30 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:Snr1; FB:FBgn0011715
inferred from physical interaction with UniProtKB:O77051
(assigned by UniProt )
inferred from physical interaction with FLYBASE:Dp; FB:FBgn0011763
inferred from physical interaction with FLYBASE:E2f1; FB:FBgn0011766
inferred from physical interaction with FLYBASE:E2f2; FB:FBgn0024371
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
Biological Process (20 terms)
Terms Based on Experimental Evidence (16 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by BHF-UCL )
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
(assigned by BHF-UCL )
inferred from mutant phenotype
(assigned by BHF-UCL )
inferred from direct assay
inferred from mutant phenotype
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
Cellular Component (5 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000348425
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the retinoblastoma protein (RB) family. (Q24472)
Summaries
Protein Function (UniProtKB)
Functions in cell cycle regulation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In follicle cells, the complex plays a central role in the site-specific DNA replication at the chorion loci. During development, the complex represses transcription of developmentally controlled E2F target genes.
(UniProt, Q24472)
Summary (Interactive Fly)

cell cycle regulator - interacts with and negatively regulates members of a family of factors called E2Fs, which serve to activate transcription of genes required for entry into S phase - forms the DREAM complex, which regulates cell cycle regulators, apoptosis regulators, and DNA replication/repair factors - binds chromosomal proteins and modulates genome stability - negatively regulated by CycE-Cdk2 and CycD-Cdk4 cyclin-dependent kinase complexes

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\Rbf or the JBrowse view of Dmel\Rbf for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0070146
3619
845
Additional Transcript Data and Comments
Reported size (kB)

2.593 (unknown)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0070141
96.8
845
6.54
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

797 (aa); 95 (kD observed)

Comments
External Data
Subunit Structure (UniProtKB)

Forms a complex with the DRTF1/E2F transcription factor through binding to a C-terminal region of E2F. This binding inhibits the E2F-mediated transactivation activity. Component of the DREAM complex at least composed of Myb, Caf1, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, Rpd3 and l(3)mbt.

(UniProt, Q24472)
Post Translational Modification

Phosphorylation by cyclin E-dependent kinases appears to negatively regulate RBF activity.

(UniProt, Q24472)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rbf using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Rbf is highly expressed in proliferating myoblasts at early stage of pupal development as myoblasts are migrating to fuse with forming myotubes. At 14hr APF, the level of Rbf is strikingly lower in myotubes compared to the surrounding pool of migrating myoblasts. Levels remain low in myotubes until fusion is complete and no myoblasts are detectable at 33hr APF. Later in pupal development, Rbf is detected in dissected DLMs at 52hr APF and remains at 80 and 96hr APF. It appears that actively proliferating myoblasts express high levels of Rbf, whereas the expression of Rbf is dramatically reduced as myoblasts undergo fusion to form myotubes.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rbf in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 22 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 42 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rbf
Transgenic constructs containing regulatory region of Rbf
Deletions and Duplications ( 7 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
embryonic epidermis & S phase
embryonic epidermis & S phase | germ-line clone
follicle cell & cell cycle (with Rbf11)
follicle cell & cell cycle (with Rbf120a)
S phase | ectopic & eye disc | posterior (with Rbf11)
S phase | ectopic & eye disc | posterior (with Rbf120a)
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
14 of 15
Yes
Yes
4 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
14 of 15
Yes
Yes
14 of 15
Yes
Yes
4 of 15
No
No
1  
Rattus norvegicus (Norway rat) (3)
13 of 13
Yes
Yes
7 of 13
No
Yes
4 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
9 of 12
Yes
Yes
8 of 12
No
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
14 of 15
Yes
Yes
9 of 15
No
Yes
4 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
13 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
6 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919029U )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915024B )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W01WD )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X01TZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0398 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (1)
7 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 3 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
suppressible
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Subunit Structure (UniProtKB)
Forms a complex with the DRTF1/E2F transcription factor through binding to a C-terminal region of E2F. This binding inhibits the E2F-mediated transactivation activity. Component of the DREAM complex at least composed of Myb, Caf1, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, Rpd3 and l(3)mbt.
(UniProt, Q24472 )
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Genomic Location and Detailed Mapping Data
Chromosome (arm)
X
Recombination map

1-0

Cytogenetic map
Sequence location
X:797,090..801,479 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
1C5-1C5
Limits computationally determined from genome sequence between P{EP}sdkEP369 and P{EP}EP1600&P{EP}EP1498
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
1C-1D
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (24)
Genomic Clones (7)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (218)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: Rbf CG7413

Source for database merge of
Additional comments
Other Comments

gig and Rbf synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased reactive oxygen species enzyme induction.

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

E2f2 as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development.

Rbf is required at multiple stages of development. Most, if not all, of the function of Rbf during development is mediated through E2f.

Rbf is dispensable for early cell cycles in the embryo, but plays an essential role in the introduction of G1 control during development.

Identification: Yeast two hybrid screen for proteins that interact with E2f.

Rbf plays a critical role in the regulation of cell proliferation.

Overexpression of dap during eye development interferes with cell cycle progression and interacts genetically with Rbf and CycE.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 51 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
KEGG Genes - Molecular building blocks of life in the genomic space.
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DPiM - Drosophila Protein interaction map
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (25)
Reported As
Symbol Synonym
Rbf
(Graves et al., 2020, Hinnant et al., 2020, Murcia et al., 2019, Ramanathan et al., 2019, Shokri et al., 2019, van den Ameele and Brand, 2019, Akishina et al., 2018, Ariss et al., 2018, Bischof et al., 2018, Gene Disruption Project members, 2018-, Lee et al., 2018, Song et al., 2018, Tamirisa et al., 2018, Akishina et al., 2017, Romero-Pozuelo et al., 2017, Transgenic RNAi Project members, 2017-, Zhang et al., 2017, Dominado et al., 2016, Yadav et al., 2016, Bouleau and Tricoire, 2015, Doggett et al., 2015, Haelterman, 2015.3.20, Schertel et al., 2015, Sreedharan et al., 2015, Tokusumi et al., 2015, Wang and Baker, 2015, Lee et al., 2014, Liang et al., 2014, Rhee et al., 2014, Tipping and Perrimon, 2014, Truscott et al., 2014, Bischof et al., 2013, Bonke et al., 2013, Das et al., 2013, Kwon et al., 2013, Lim et al., 2013, Pomerantz and Blau, 2013, Schertel et al., 2013, Yamamoto et al., 2013-, Adamson and Lajeunesse, 2012, Japanese National Institute of Genetics, 2012.5.21, Zhai et al., 2012, Axelsson et al., 2011, Gordon and Du, 2011, Klusza and Deng, 2011, Miles et al., 2011, Toku et al., 2011, Herranz et al., 2010, Herz et al., 2010, Lloyd and Taylor, 2010, Nedelsky et al., 2010, Popodi et al., 2010-, Swaminathan et al., 2010, Venken et al., 2010, Wasbrough et al., 2010, Ahlander and Bosco, 2009, Diangelo and Birnbaum, 2009, Jiang et al., 2009, Metzendorf et al., 2009, Ogura et al., 2009, Steele et al., 2009, Suzuki et al., 2009, Oktaba et al., 2008, Doggett et al., 2007, Ringrose and Paro, 2007, Straub and Becker, 2007, Tadros et al., 2007, Axelson, 2006, Morris et al., 2006, Laviolette et al., 2005, Thomas, 2005)
Name Synonyms
retinoblastoma
retinoblastoma factor
retinoblastoma factor-1
retinoblastoma family protein 1
retinoblastoma-family homolog
retinoblastoma-family protein
Secondary FlyBase IDs
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (327)