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Gene Dmel\Rbf

General Information
Symbol	Dmel\Rbf	Species	D. melanogaster
Name	Retinoblastoma-family protein	Annotation symbol	CG7413
Feature type	protein_coding_gene	FlyBase ID	FBgn0015799
Gene Model Status	Current	Stock availability	9 publicly available
Also Known As	RBF1, Rb, EG:34F3.3, dRBF
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	1C5-1C5	Sequence location	X:691,121..695,490 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Retinoblastoma-family protein is referred to in FlyBase by the symbol Dmel\Rbf (CG7413, FBgn0015799). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; transcription factor binding; transcription regulatory region DNA binding. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; cell cycle; cell cycle process; negative regulation of cellular biosynthetic process; regulation of apoptosis; negative regulation of metabolic process; negative regulation of biosynthetic process; regulation of cell proliferation; regulation of S phase of mitotic cell cycle; regulation of DNA binding. 58 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; organ system; external compound sense organ; thoracic segment; integumentary plate; cell part; non-connected developing system; cell cycle; imaginal precursor. It has one annotated transcript and one annotated polypeptide. Protein features are: Cyclin-like; Domain of unknown function DUF3452, retinoblastoma-associated; Retinoblastoma-associated protein, A-box; Retinoblastoma-associated protein, B-box. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1623479_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rbf. Gene sequence location is X:691121..695490.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	References Manning et al., 2010, Genes Dev. 24(13): 1364--1376
FB2012_01	References Hsieh et al., 2010, PLoS Genet. 6(8): Sukhanova et al., 2011, Dev. Biol. 359(2): 190--198 Sequence features BKN22455 HFA18704 Controlled Vocabulary Terms protein binding regulation of cell proliferation
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 1C5-1C5   Limits computationally determined from genome sequence between P{EP}sdkEP369 and P{EP}EP1600&P{EP}EP1498
Experimentally Determined Cytological Location
Cytogenetic map Notes References 1C-1D   (determined by in situ hybridisation)   (Du and Dyson, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rbf for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone GH05946 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rbf-RA FBtr0070146  NM_080297   3599   845
Additional Transcript Data & Comments
Reported size (kB)	2.593 (unknown) (Dougan and DiNardo, 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rbf-PA   FBpp0070141   96.8   845   6.54     NP_525036   AAF45551
Additional Polypeptide Data & Comments
Reported size (kDa)	797 (aa); 95 (kD observed) (Dougan and DiNardo, 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Retinoblastoma-associated protein, B-box (IPR002719) Retinoblastoma-associated protein, A-box (IPR002720) Cyclin-like (IPR013763) Domain of unknown function DUF3452, retinoblastoma-associated (IPR024599)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202591   AA203029   AA941205   AE014298 AAF45551   AI108022   AI294097   AI513651   AI534641   AI543807   AW940215   AY047549 AAK77281   AY089532 AAL90270   BF491751   BI166421   BI167122   BI214277   BI215455   BI353656   BI363309   BI364231   BI365813   CL527993   CO268940   CO269421   CO294071   CO310357   EC082551   EC197300   EC207009   EC225098   EV586176   GH748026   GH748846   GH749802   GH751627   GH753846   GH755517   GH755799   GH755880   GH756104   GH756393   GH756703   GH757696   GH757894   GH758493   GH758749   GH759206   GH770803   GH770880   GH772236   GH772338   GH774327   GH776831   GH777168   GH777309   GH777585   GH780184   GH780531   GH781209   GH781557   GH782131   GH783420   GH783955   GH784464   GH784499   GH784785   GH785683   GH785809   GH787233   GH787360   GH787400   GH787943   GH788877   GH789575   GH789714   GH790135   GH792497   GH793060   GH794728   GH794863   GH795226   GH795282   GH796484   GH796991   GH797091   GH799520   GH799779   GH799988   GH801556   GH802176   GH802559   GH803271   GH805398   GH806011   GH806722   GH807188   GH808264   GH808697   GH810154   GH810410   GH810639   GH810665   GH811956   GH812840   GH813404   GH814688   GH814880   GH815082   GH817875   GH818819   GH818893   GH820982   GH821554   GH821749   GH822034   GH824585   GH825387   GH826687   GH827254   GH829488   GH830339   GH834855   GH835921   GH835944   GH835955   GH836208   GH836624   GH838616   GH838631   GH839694   GH843178   GH844214   GH844455   GH844468   GH845056   GH845899   GH846838   GH851399   GH853910   GH853956   GH854031   GH854173   GH854380   GH856214   GH856751   GH857462   GH859951   GH860135   GH861758   GH862205   GH862345   GH862981   GH863420   GH865597   GH867342   GH867566   GH867755   GH868639   GH869873   GH870475   GH871058   GH871316   GH871718   GH871906   GH872489   GH873225   GH874100   GH879983   GH881001   GH881358   GH881468   GH882684   GH883045   GH883794   GH884766   GH885360   GH885462   GH885798   GH887094   GH888810   GH890077   GH895451   GH895914   GH897117   GH897781   GH897952   GH899370   GH900903   GH901337   GH904442   GH908698   GH909379   GH909384   GH909945   GH911252   GH912228   GH912722   GH914206   GH915277   GH916533   GH917288   GH917353   GH917495   GH921779   GH922686   GH923206   GH926469   GH926482   GH926805   GH927100   GH927596   GH929920   GH932194   GH933329   GH933696   GH934044   GH934678   GH935325   GH937142   GH937699   GH937778   GH938474   GH939818   GH940650   X96975 CAA65661
UniProtKB/Swiss-Prot	Q24472
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0015799    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0015799 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0015799 expression level Developmental Stage   Expression Level embryo 00-02hr    8378 embryo 02-04hr    1827 embryo 04-06hr    3645 embryo 06-08hr    3927 embryo 08-10hr    2767 embryo 10-12hr    2094 embryo 12-14hr    1417 embryo 14-16hr    1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1    1691 larva L2    1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr    1636 white prepupae 24hr    1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day    2626 adult female 05day    4503 adult female 30day    4809 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8378) embryo 02-04hr  (1827) embryo 04-06hr  (3645) embryo 06-08hr  (3927) embryo 08-10hr  (2767) embryo 10-12hr  (2094) embryo 12-14hr  (1417) embryo 14-16hr  (1457) embryo 16-18hr  (1267) embryo 18-20hr  (754) embryo 20-22hr  (641) embryo 22-24hr  (604) larva L1  (1691) larva L2  (1521) larva L3 12hr old  (1068) larva L3 puffstage 1-2  (766) larva L3 puffstage 3-6  (1175) larva L3 puffstage 7-9  (1286) white prepupae new  (1345) white prepupae 12hr  (1636) white prepupae 24hr  (1755) pupae 2d postWPP  (1003) pupae 3d postWPP  (642) pupae 4d postWPP  (633) adult male 01day  (798) adult male 05day  (1005) adult male 30day  (976) adult female 01day  (2626) adult female 05day  (4503) adult female 30day  (4809) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8378) embryo 02-04hr  (1827) embryo 04-06hr  (3645) embryo 06-08hr  (3927) embryo 08-10hr  (2767) embryo 10-12hr  (2094) embryo 12-14hr  1417 embryo 14-16hr  1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1  (1691) larva L2  (1521) larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr  (1636) white prepupae 24hr  (1755) pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day  (2626) adult female 05day  (4503) adult female 30day  (4809) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8378 embryo 02-04hr    1827 embryo 04-06hr    3645 embryo 06-08hr    3927 embryo 08-10hr    2767 embryo 10-12hr    2094 embryo 12-14hr    1417 embryo 14-16hr    1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1    1691 larva L2    1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr    1636 white prepupae 24hr    1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day    2626 adult female 05day    4503 adult female 30day    4809 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8378 embryo 02-04hr    1827 embryo 04-06hr    3645 embryo 06-08hr    3927 embryo 08-10hr    2767 embryo 10-12hr    2094 embryo 12-14hr    1417 embryo 14-16hr    1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1    1691 larva L2    1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr    1636 white prepupae 24hr    1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day    2626 adult female 05day    4503 adult female 30day    4809 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0015799 expression level Developmental Stage   Expression Level embryo 00-02hr    8378 embryo 02-04hr    1827 embryo 04-06hr    3645 embryo 06-08hr    3927 embryo 08-10hr    2767 embryo 10-12hr    2094 embryo 12-14hr    1417 embryo 14-16hr    1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1    1691 larva L2    1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr    1636 white prepupae 24hr    1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day    2626 adult female 05day    4503 adult female 30day    4809 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (8378) embryo 02-04hr  (1827) embryo 04-06hr  (3645) embryo 06-08hr  (3927) embryo 08-10hr  (2767) embryo 10-12hr  (2094) embryo 12-14hr  (1417) embryo 14-16hr  (1457) embryo 16-18hr  (1267) embryo 18-20hr  (754) embryo 20-22hr  (641) embryo 22-24hr  (604) larva L1  (1691) larva L2  (1521) larva L3 12hr old  (1068) larva L3 puffstage 1-2  (766) larva L3 puffstage 3-6  (1175) larva L3 puffstage 7-9  (1286) white prepupae new  (1345) white prepupae 12hr  (1636) white prepupae 24hr  (1755) pupae 2d postWPP  (1003) pupae 3d postWPP  (642) pupae 4d postWPP  (633) adult male 01day  (798) adult male 05day  (1005) adult male 30day  (976) adult female 01day  (2626) adult female 05day  (4503) adult female 30day  (4809) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (8378) embryo 02-04hr  1827 embryo 04-06hr  (3645) embryo 06-08hr  (3927) embryo 08-10hr  (2767) embryo 10-12hr  2094 embryo 12-14hr  1417 embryo 14-16hr  1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1  1691 larva L2  1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr  1636 white prepupae 24hr  1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day  (2626) adult female 05day  (4503) adult female 30day  (4809) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    8378 embryo 02-04hr    1827 embryo 04-06hr    3645 embryo 06-08hr    3927 embryo 08-10hr    2767 embryo 10-12hr    2094 embryo 12-14hr    1417 embryo 14-16hr    1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1    1691 larva L2    1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr    1636 white prepupae 24hr    1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day    2626 adult female 05day    4503 adult female 30day    4809 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    8378 embryo 02-04hr    1827 embryo 04-06hr    3645 embryo 06-08hr    3927 embryo 08-10hr    2767 embryo 10-12hr    2094 embryo 12-14hr    1417 embryo 14-16hr    1457 embryo 16-18hr    1267 embryo 18-20hr    754 embryo 20-22hr    641 embryo 22-24hr    604 larva L1    1691 larva L2    1521 larva L3 12hr old    1068 larva L3 puffstage 1-2    766 larva L3 puffstage 3-6    1175 larva L3 puffstage 7-9    1286 white prepupae new    1345 white prepupae 12hr    1636 white prepupae 24hr    1755 pupae 2d postWPP    1003 pupae 3d postWPP    642 pupae 4d postWPP    633 adult male 01day    798 adult male 05day    1005 adult male 30day    976 adult female 01day    2626 adult female 05day    4503 adult female 30day    4809 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0015799    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0015799 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0015799 expression level Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0015799 expression level Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body no informative data Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop no informative data Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass no informative data Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body no informative data Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop no informative data Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca no informative data Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass no informative data FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    45.1   NA  Eye    35.5   NA  Brain    107.2   158.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    61.9   NA  Crop    no informative data   57  Midgut    46.8   78.6  Hindgut    40.4   28.8  Malpighian Tubules    51.4   no informative data  Fat Body    41.3   136.1  Salivary Gland    80.8   NA  Heart    30.075   91.575  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    37.9   NA  Ovary    381.2   NA  Testis    83.2   NA  Male Accessory Gland    45.2   82.625  Carcass    no informative data modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015799 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7413-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rbf Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4ap-md544 RbfScer\UAS.cDa lethal, with Scer\GAL4ey.PH Rbf280.Scer\UAS lethal | germline clone | rescuable maternal effect Rbfunspecified lethal | partially, with Scer\GAL4ey.PH RbfK774A.Scer\UAS RbfK774R.Scer\UAS lethal | recessive Rbf11 Rbf14 Rbf16 viable Rbf15a Rbf38a Rbf120a RbfEY07209 viable, with Scer\GAL4pnr-MD237 RbfGD4484 viable (with Rbf11) Rbf120a viable (with Rbf120a) Rbf11 Sterility Allele fertile RbfEY07209 sterile (with Rbf11) Rbf120a sterile (with Rbf120a) Rbf11 Other Phenotypes Allele cell growth defective | pupal stage | somatic clone Rbf15aΔ decreased cell number, with Scer\GAL4sca-537.4 RbfScer\UAS.cDa developmental rate defective, with Scer\GAL4hs.PH RbfScer\UAS.cDa increased cell death (with Rbf15aΔ) Rbf120-3-3 increased cell death (with Rbf120-3-3) Rbf15aΔ increased cell death | recessive Rbf120a increased cell death | recessive | somatic clone Rbf120a RbfΔ14 increased cell death | somatic clone Rbf14 Rbf15aΔ Rbf120a increased cell death | somatic clone | third instar larval stage Rbf15aΔ increased cell death | somatic clone | wandering third instar larval stage RbfΔ14 increased cell number | somatic clone | third instar larval stage Rbf15aΔ mitotic cell cycle defective | somatic clone, with Scer\GAL4Act5C.PP RbfScer\UAS.cDa neuroanatomy defective, with Scer\GAL4sca-537.4 RbfScer\UAS.cDa visible Rbf280.GMR RbfGMR.PD visible, with Scer\GAL4en-e16E RbfScer\UAS.cDa visible, with Scer\GAL4ey.PH Rbf1-845.Scer\UAS Rbf6KA.Scer\UAS Rbf10.Scer\UAS Rbf16.Scer\UAS Rbf223.Scer\UAS RbfK754A.Scer\UAS RbfK774A.Scer\UAS RbfK774R.Scer\UAS RbfScer\UAS.cDa visible, with Scer\GAL4GMR.PF RbfGD4484 visible, with Scer\GAL4hs.PB RbfScer\UAS.cDa visible, with Scer\GAL4pnr-MD237 RbfGD4484 visible, with Scer\GAL4vg.PM RbfScer\UAS.cDa wild-type Rbfhs.PD Phenotype manifest in Allele abdomen, with Scer\GAL4hs.PH RbfScer\UAS.cDa anterior crossvein, with Scer\GAL4dpp.blk1 RbfScer\UAS.cDa chaeta, with Scer\GAL4hs.PB RbfScer\UAS.cDa chorion (with Rbf11) Rbf120a chorion (with Rbf120a) Rbf11 embryonic/larval midgut primordium Rbf14 embryonic epidermis & S phase Rbfunspecified embryonic epidermis & S phase | germ-line clone Rbfunspecified embryonic epidermis | embryonic stage 13 Rbf14 eye Rbf14 Rbf280.GMR RbfGMR.PD eye, with Scer\GAL4ey.PH Rbf10.Scer\UAS Rbf16.Scer\UAS Rbf223.Scer\UAS RbfScer\UAS.cDa Rbf1-845.Scer\UAS RbfK774R.Scer\UAS RbfK774A.Scer\UAS Rbf6KA.Scer\UAS RbfK754A.Scer\UAS eye (with Rbf11) Rbf120a eye (with Rbf120a) Rbf11 eye | ectopic | somatic clone Rbf14 eye | somatic clone Rbf14 Rbf15aΔ eye disc Rbf120a Rbf280.GMR eye disc, with Scer\GAL4ey.PH RbfScer\UAS.cDa eye disc (with Rbf11) Rbf120a eye disc (with Rbf120a) Rbf11 eye disc | somatic clone RbfΔ14 Rbf120a eye disc | somatic clone | third instar larval stage Rbf15aΔ eye disc | third instar larval stage (with Rbf15aΔ) Rbf120-3-3 eye disc | third instar larval stage (with Rbf120-3-3) Rbf15aΔ eye disc anterior to the morphogenetic furrow | somatic clone Rbf120a eye disc posterior to the morphogenetic furrow | somatic clone | third instar larval stage Rbf15aΔ follicle cell (with Rbf14) Rbf120a follicle cell (with Rbf120a) Rbf14 follicle cell & cell cycle (with Rbf11) Rbf120a follicle cell & cell cycle (with Rbf120a) Rbf11 interommatidial bristle Rbf280.GMR RbfGMR.PD interommatidial bristle, with Scer\GAL4GMR.PF RbfGD4484 interommatidial bristle | somatic clone Rbf14 mesothoracic tergum, with Scer\GAL4pnr-MD237 RbfGD4484 mitotic cell cycle (with Rbf14) Rbf120a mitotic cell cycle (with Rbf120a) Rbf14 mitotic cell cycle | somatic clone, with Scer\GAL4Act5C.PP RbfScer\UAS.cDa morphogenetic furrow | somatic clone Rbf120a ommatidium Rbf14 Rbf280.GMR RbfGMR.PD ommatidium | pupal stage | somatic clone Rbf15aΔ ommatidium | somatic clone Rbf14 pigment cell RbfGMR.PD scutellar bristle Rbf14 S phase | ectopic Rbfunspecified S phase | ectopic & eye disc | posterior (with Rbf11) Rbf120a S phase | ectopic & eye disc | posterior (with Rbf120a) Rbf11 submarginal cell, with Scer\GAL4dpp.blk1 RbfScer\UAS.cDa wing | posterior compartment, with Scer\GAL4en-e16E RbfScer\UAS.cDa wing disc | somatic clone | wandering third instar larval stage RbfΔ14 wing margin, with Scer\GAL4vg.PM RbfScer\UAS.cDa
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of Rbf Class Mutagen Stocks Known lesion Rbf14 amorphic allele - genetic evidence Delta2-3 1 Yes Rbfcas-21 ethyl methanesulfonate 1 Yes Rbfd00614 1 -- RbfEY07209 P-element activity 1 -- RbfGS5173 1 -- Rbfsls-15 X ray 1 Yes Rbf11 Delta2-3 0 Yes Rbf120-3-3 0 -- Rbf120a Delta2-3 0 Yes Rbf15a Delta2-3 0 Yes Rbf15aΔ P-element activity 0 Yes Rbf16 Delta2-3 0 Yes Rbf38a Delta2-3 0 Yes RbfCAS-21 0 -- RbfGG01588 0 -- RbfSLS-15 0 Yes Rbfunspecified 0 -- RbfΔ14 loss of function allele 0 --
Alleles Carried on Transgenic Constructs ( 40 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rbf Class Mutagen Stocks Known lesion Rbft.Exel in vitro construct - genomic fragment 2 Yes RbfGD4484 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rbf1-717.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Rbf1-845.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf10.cXa in vitro construct - amino acid replacement 0 Yes Rbf10.hs in vitro construct 0 Yes Rbf10.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf16.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf223.cXa in vitro construct - amino acid replacement 0 Yes Rbf223.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf280.cXa in vitro construct - amino acid replacement 0 Yes Rbf280.GMR in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Rbf280.hs in vitro construct 0 Yes Rbf280.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf30.cXa in vitro construct - amino acid replacement 0 Yes Rbf30.hs in vitro construct 0 Yes Rbf30.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf555L.hs in vitro construct 0 Yes Rbf555L.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf596W.hs in vitro construct 0 Yes Rbf596W.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf6KA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfAct5C.PD in vitro construct - regulatory fusion 0 Yes RbfcFa in vitro construct 0 Yes RbfdsRNA.cFa in vitro construct - RNAi 0 Yes RbfdsRNA.cKa in vitro construct - RNAi 0 Yes RbfdsRNA.cLa in vitro construct - RNAi 0 Yes RbfGMR.PD in vitro construct - regulatory fusion 0 Yes Rbfhs.PD in vitro construct - regulatory fusion 0 Yes Rbfie1.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RbfK754A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfK774A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfK774R.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfM4.cXa in vitro construct - amino acid replacement 0 Yes RbfN.Scer\UAS.P\T.T:Disc\RFP-mCherry,T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RbfScer\UAS.cDa in vitro construct - regulatory fusion 0 Yes RbfT:Zzzz\FLAG in vitro construct - regulatory fusion 0 Yes RbfWT.T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes RbfΔ728-786.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes RbfΔIE.T:Zzzz\FLAG in vitro construct - deletion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)AD11 (Du and Dyson, 1999) Df(1)Exel6223 (Ryder, 2004.4.26) Df(1)su(s)83 (Du and Dyson, 1999)
Not disrupted in	Df(1)A94 (Du and Dyson, 1999) Df(1)S39 (Du and Dyson, 1999)
Duplicated in	Dp(1;3)DC012 (Popodi et al., 2010-)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4484} NA P{UASp-Rbf.N.RFP} NA P{UAS-Rbf.1-717} NA P{UAS-Rbf.1-845} NA P{UAS-Rbf.6KA} NA P{UAS-Rbf.10} NA P{UAS-Rbf.16} NA P{UAS-Rbf.30} NA P{UAS-Rbf.223} NA P{UAS-Rbf.280} NA P{UAS-Rbf.555L} NA P{UAS-Rbf.596W} NA P{UAS-Rbf.D} NA P{UAS-Rbf.K754A} NA P{UAS-Rbf.K774A} NA P{UAS-Rbf.K774R} NA P{UAS-Rbf.Δ728-786} NA heat-shock construct P{hs-Rbf.30} NA P{hs-Rbf.280} NA P{hs-Rbf.555L} NA P{hs-Rbf.596W} NA P{hs-Rbf.D} NA P{hs-Rbf.X} NA characterization construct P{GMR-Rbf.280} NA P{GMR-Rbf} NA P{Rbf.WT.FLAG} NA P{Rbf.ΔIE.FLAG} NA P{Rbf-FRT.2.1} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}RbfEY07209 NA P{GSV2}RbfGS5173 NA P{Mae-UAS.6.11}RbfGG01588 NA P{XP}Rbfd00614 NA miscellaneous insertions P{CaSpeR}Rbf15a NA P{CaSpeR}Rbf38a NA P{CaSpeR}Rbf120a NA
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Snr1 (Brumby et al., 2002) transcription factor binding inferred from physical interaction with Dp (Stevaux et al., 2002) inferred from physical interaction with Dp AND inferred from physical interaction with E2f (Du et al., 1996) inferred from physical interaction with E2f2 (Stevaux et al., 2002) inferred from physical interaction with E2f (Stevaux et al., 2002) transcription regulatory region DNA binding inferred from direct assay (Ullah et al., 2007)
Biological Process
CV term Evidence References
DNA damage response, signal transduction resulting in induction of apoptosis inferred from mutant phenotype (Moon et al., 2008) DNA endoreduplication inferred from mutant phenotype (Shcherbata et al., 2004) G1 phase of mitotic cell cycle inferred from mutant phenotype (Du and Dyson, 1999) mitotic cell cycle G2/M transition DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) negative regulation of apoptosis inferred from mutant phenotype (Tanaka-Matakatsu et al., 2009) negative regulation of DNA replication inferred from mutant phenotype (Bosco et al., 2001) negative regulation of gene expression inferred from mutant phenotype (Lu et al., 2007) negative regulation of S phase of mitotic cell cycle inferred from mutant phenotype (Firth and Baker, 2005) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (Du et al., 1996, Stevaux et al., 2002) inferred from genetic interaction with Rbf2 (Lewis et al., 2004) inferred from mutant phenotype (Du and Dyson, 1999, Korenjak et al., 2004) regulation of cell cycle inferred from mutant phenotype (Du and Dyson, 1999) regulation of cell proliferation inferred from mutant phenotype (Hsieh et al., 2010) regulation of DNA binding inferred from direct assay (Du et al., 1996)
Cellular Component
CV term Evidence References
chromatin inferred from direct assay (Korenjak et al., 2004) Myb complex inferred from direct assay (Lewis et al., 2004, Korenjak et al., 2004) nucleus inferred from direct assay (Acharya et al., 2010) Rb-E2F complex inferred from direct assay (Du et al., 1996, Stevaux et al., 2002)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 2000.5.30)
Biological Process
CV term Evidence References
eggshell chorion gene amplification traceable author statement (McNairn and Gilbert, 2003, Lee and Orr-Weaver, 2003) endomitotic cell cycle traceable author statement (Lee and Orr-Weaver, 2003) negative regulation of cell proliferation non-traceable author statement (Potter and Xu, 2001) regulation of cell cycle inferred from sequence or structural similarity with human RBL1 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 2000.5.30) Rb-E2F complex traceable author statement (Lee and Orr-Weaver, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rbf allele Gene References Rbf11 Cdk4 (Meyer et al., 2000) Rbf14 Cdk4 (Meyer et al., 2000) E2f (Du and Dyson, 1999, Du, 2000) Rbf120a E2f (Du and Dyson, 1999, Du, 2000) Rbf280.GMR dap (Weng et al., 2003) RbfdsRNA.cFa E2f (Frolov et al., 2003) RbfGMR.PD CycE (Secombe et al., 1998) dap (de Nooij et al., 1996, Weng et al., 2003) Nos (Kuzin et al., 2000) ptc (Duman-Scheel et al., 2002) RbfScer\UAS.cDa Cdk4 (Datar et al., 2000) CycD (Datar et al., 2000) unspecified Dp (Kuzin et al., 2000) E2f (Kuzin et al., 2000) Nsf2 (Laviolette et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57595 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015799 DroID - A comprehensive database of gene and protein interactions. • FBgn0015799 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31027
Orthologs
Genome-wide drosophilid orthologs	Dana\GF22059 Dere\GG12742 Dgri\GH12452 Dmoj\GI14825 Dper\GL14434 Dpse\GA20332 Dsec\GM19021 Dsim\GD16459 Dvir\GJ19490 Dwil\GK19998 Dyak\GE16567
Curated drosophilid orthologs	Dpse\GA20332
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL000128-PA Aedes aegypti (mosquito) AGAP006477-PA Anopheles gambiae (mosquito) XP_395096.3 Apis mellifera (honey bee) XP_001943094.1 Acyrthosiphon pisum (pea aphid) At3g12280.1 Arabidopsis thaliana (wall cress) 218889 Branchiostoma floridae (lancelet) XP_001891849.1 Brugia malayi (roundworm nematode) BGIBMGA001949-PA Bombyx mori (silk worm) ENSBTAP00000015333 Bos taurus (bovine) CBP35378 Caenorhabditis briggsae (nematode) CE24823 Caenorhabditis elegans (worm) ENSCAFP00000012814 Canis familiaris (dog) ENSCINP00000009480 Ciona intestinalis (sea squirt) JA11263 Caenorhabditis japonica (nematode) CMT038C Cyanidioschyzon merolae (red alga) CPIJ017294 Culex pipens (mosquito) ENSCPOP00000001216 Cavia porcellus (guinea pig) RP48720 Caenorhabditis remanei (nematode) ENSCSAVP00000006505 Ciona savignyi (sedentary tunicate) 192080 Capitella spl (polychaete worm) 322171 Daphnia pulex (daphnia) ENSDARP00000067923 Danio rerio (zebra fish) ENSECAP00000021871 Equus caballus (horse) ENSGACP00000000033 Gymnopilus aculeatus (mushroom) ENSGALP00000002030 Gallus gallus (chicken) ENSP00000362768 Homo sapiens (human) ISCW002848-PA Ixodes scapularis (deer tick) 227069 Lottia gigantea (owl limpet) ENSMODP00000016476 Monodelphis domestica (gray opossum) ENSMMUP00000018886 Macaca mulatta (rhesus monkey) ENSMUSP00000029170 Mus musculus (house mouse) 239885 Nematostella vectensis (starlet anemone) XP_001604637.1 Nasonia vitripennis (wasp) ENSOANP00000021156 Ornithorynchus anatinus (platypus) ENSORLP00000023819 Oryzias latipes (japanese killifish) LOC_Os08g42600.1 Oryza sativa (asian rice) LOC_Os11g32900.1 Oryza sativa (asian rice) 24582 Ostreococcus tauri (green alga) PHUM003119-PA Pediculus humanus (human body louse) 10529 Physcomitrella patens (moss) ENSPPYP00000012262 Pongo pygmaeus (orangutan) 135906 Phytophthora sojae (root rot pathogen) ENSPTRP00000023126 Pan troglodytes (chimpanzee) ENSRNOP00000009528 Rattus norvegicus (norway rat) 4764709 Sorghum bicolor (sorghum) Smp_024900 Schistosoma mansoni (trematode parasite) XP_001186489.1 Strongylocentrotus purpuratus (purple sea urchin) 59859 Trichoplax adhaerens (metazoa) XP_971815.1 Tribolium castaneum (red flour beetle) ENSTNIP00000004043 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000031719 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000000730 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16829 NV15596 LH24347 ISCW008145 ISCW002848 FBgn0234082 FBgn0221995 FBgn0206633 FBgn0188074 FBgn0173924 JGI_V11_322171 FBgn0080327 FBgn0152039 FBgn0137576 FBgn0119931 FBgn0105029 FBgn0099058 CPIJ017294 BGIBMGA001949 ACYPI008053 GB19508 AGAP006477 AAEL000128 PHUM177400 GLEAN_09105
Stocks & Reagents
Stocks Listed in FlyBase ( 9 )
Bloomington	8342 Rbfcas-21/FM7c 7435 Rbf14 w1118/FM7c 8298 y1 w1118; P{Rbf-FRT.2.1}3 8301 y1 w1118; P{Rbf-FRT.2.1}2 16803 y1 P{EPgy2}RbfEY07209 w67c23 8343 Rbfsls-15 w1118 P{neoFRT}18A/FM7c, P{ftz/lacC}YH1
Harvard	- P{XP}Rbfd00614
Kyoto	200757 w* P{GSV2}RbfGS5173/Binsinscy
VDRC	v10696 w1118; P{GD4484}v10696
Genomic Clones ( 1 )
	BACR19J01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 219 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH05946 LD02906
Other clones	DPiM_LD02906
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT28564 GM24583 LD03488 LD25209 LD45859 RE06540 RE07437 RE20040 RE21857 RE48083 RE49295 RE51195 SD07569 SD10719
Other clones	98413 CK01.61B.E6 CK01.122A.H11 CK01.124B.H10 Dmel_dig_000818_0651_0404 EK093132 EK094463 EK205901 EK221322 RP003007473 RP003014952 RP003016838 RP003018249 RP003020227 RP003021842 RP003032437 RP003038722 RP003038744 RP003039338 RP003040497 RP003048962 RP003053931 RP003055806 RP003063373 RP003063728 RP003064578 RP003070067 RP003071617 RP003076144 RP003081530 RP003083877 RP003086177 RP003088510 RP003091242 RP003097109 RP003099729 RP003120932 RP003121765 RP003124958 RP003125444 RP003129114 RP003133139 RP003134490 RP003138887 RP003147023 RP003151410 RP003154788 RP003157920 RP003159253 RP003160024 RP003160274 RP003160762 RP003162892 RP003165674 RP003169478 RP003171340 RP003171912 RP003180117 RP003180877 RP003192420 RP003196086 RP003196185 RP003203252 RP003203387 RP003204158 RP003204174 RP003206636 RP003207595 RP003208902 RP003217443 RP003218458 RP003219580 RP003224439 RP003226489 RP003227727 RP003230226 RP003231730 RP003233746 RP003234035 RP003240312 RP003242209 RP003242929 RP003244571 RP003247560 RP003249223 RP003251921 RP003256461 RP003260328 RP003261082 RP003262329 RP003263323 RP003266747 RP003269190 RP003277780 RP003277952 RP003280804 RP003283988 RP003284547 RP003285741 RP003285979 RP003288314 RP003292781 RP003293241 RP003294278 RP003299052 RP003306547 RP003306746 RP003307308 RP003309916 RP003310005 RP003314390 RP003315023 RP003317444 RP003319272 RP003321200 RP003333683 RP003334185 RP003340047 RP003342931 RP003361958 RP003370691 RP003389112 RP003391961 RP003393118 RP003394145 RP003396066 RP003397633 RP003401031 RP003404645 RP003408453 RP003410610 RP003413832 RP003416855 RP003416919 RP003428549 RP003432887 RP003433228 RP003437142 RP003448890 RP003452010 RP003453719 RP003458105 RP003458613 RP003458974 RP003460266 RP003461647 RP003462648 RP003462861 RP003463529 RP003472310 RP003472414 RP003474819 RP003475031 RP003476390 RP003485063 RP003491234 RP003497319 RP003501304 RP003503642 RP003504781 RP003509540 RP003512853 RP003514057 RP003517420 RP003518071 RP003521676 RP003527366 RP003530246 RP003535940 RP003548167 RP003552110 RP003552587 RP003559753 RP003563397 RP003563796 RP003564361 RP003567823 RP003568851 RP003572905 RP003574932 RP003576998 RP003581033 RP003583594 RP003583902 RP003598026 RP003598155 RP003603188 RP003603460 RP003616372 RP003623736 RP003626233 RP003629515 RP003633079 RP003644674 RP003645960 RP003651065 RP003663232 RP003666009 RP003671956 RP003672637 RP003672794 RP003678747
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015799 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31027
Antibody Information
	polyclonal (Dougan and DiNardo, 1992, Du et al., 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rbf CG7413 (Nusskern, 1999.11)
Source for database merge of
Additional comments
Other Comments
	gig and Rbf synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased reactive oxygen species enzyme induction. (Li et al., 2010) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) E2f2 as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development. (Weng et al., 2003) Rbf is required at multiple stages of development. Most, if not all, of the function of Rbf during development is mediated through E2f. (Du, 2000) Rbf is dispensable for early cell cycles in the embryo, but plays an essential role in the introduction of G1 control during development. (Du and Dyson, 1999) Overexpression of dap during eye development interferes with cell cycle progression and interacts genetically with Rbf and CycE. (de Nooij et al., 1996) Identification: Yeast two hybrid screen for proteins that interact with E2f. (Du et al., 1996) Rbf plays a critical role in the regulation of cell proliferation. (Du et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080297 RefSeq (Proteins) • NP_525036 DDBJ / EMBL / GenBank • AA202591 AAF45551 AAK77281 AAL90270 AI108022 AI294097 AL031583 AW940215 AY047549 AY089532 CAA65661 CL527993 X96975 Entrez Gene - A searchable database of RefSeq genes. • 31027 UniProtKB/Swiss-Prot • Q24472
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Retinoblastoma-associated protein, B-box (IPR002719) Retinoblastoma-associated protein, A-box (IPR002720) Cyclin-like (IPR013763) Domain of unknown function DUF3452, retinoblastoma-associated (IPR024599)
Linkouts
	BioGRID - A database of protein and genetic interactions • 57595 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015799 DroID - A comprehensive database of gene and protein interactions. • FBgn0015799 DRSC - Results from RNAi screens. • FBgn0015799 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015799 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7413-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015799 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31027 InParanoid A subset of ortholog calls from InParanoid. • AAEL000128-PA Aedes aegypti (mosquito) AGAP006477-PA Anopheles gambiae (mosquito) XP_395096.3 Apis mellifera (honey bee) XP_001943094.1 Acyrthosiphon pisum (pea aphid) At3g12280.1 Arabidopsis thaliana (wall cress) 218889 Branchiostoma floridae (lancelet) XP_001891849.1 Brugia malayi (roundworm nematode) BGIBMGA001949-PA Bombyx mori (silk worm) ENSBTAP00000015333 Bos taurus (bovine) CBP35378 Caenorhabditis briggsae (nematode) CE24823 Caenorhabditis elegans (worm) ENSCAFP00000012814 Canis familiaris (dog) ENSCINP00000009480 Ciona intestinalis (sea squirt) JA11263 Caenorhabditis japonica (nematode) CMT038C Cyanidioschyzon merolae (red alga) CPIJ017294 Culex pipens (mosquito) ENSCPOP00000001216 Cavia porcellus (guinea pig) RP48720 Caenorhabditis remanei (nematode) ENSCSAVP00000006505 Ciona savignyi (sedentary tunicate) 192080 Capitella spl (polychaete worm) 322171 Daphnia pulex (daphnia) ENSDARP00000067923 Danio rerio (zebra fish) ENSECAP00000021871 Equus caballus (horse) ENSGACP00000000033 Gymnopilus aculeatus (mushroom) ENSGALP00000002030 Gallus gallus (chicken) ENSP00000362768 Homo sapiens (human) ISCW002848-PA Ixodes scapularis (deer tick) 227069 Lottia gigantea (owl limpet) ENSMODP00000016476 Monodelphis domestica (gray opossum) ENSMMUP00000018886 Macaca mulatta (rhesus monkey) ENSMUSP00000029170 Mus musculus (house mouse) 239885 Nematostella vectensis (starlet anemone) XP_001604637.1 Nasonia vitripennis (wasp) ENSOANP00000021156 Ornithorynchus anatinus (platypus) ENSORLP00000023819 Oryzias latipes (japanese killifish) LOC_Os08g42600.1 Oryza sativa (asian rice) LOC_Os11g32900.1 Oryza sativa (asian rice) 24582 Ostreococcus tauri (green alga) PHUM003119-PA Pediculus humanus (human body louse) 10529 Physcomitrella patens (moss) ENSPPYP00000012262 Pongo pygmaeus (orangutan) 135906 Phytophthora sojae (root rot pathogen) ENSPTRP00000023126 Pan troglodytes (chimpanzee) ENSRNOP00000009528 Rattus norvegicus (norway rat) 4764709 Sorghum bicolor (sorghum) Smp_024900 Schistosoma mansoni (trematode parasite) XP_001186489.1 Strongylocentrotus purpuratus (purple sea urchin) 59859 Trichoplax adhaerens (metazoa) XP_971815.1 Tribolium castaneum (red flour beetle) ENSTNIP00000004043 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000031719 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000000730 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/retnbls1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31027 modMine - Data generated by the modENCODE project. • FBgn0015799 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB16829 NV15596 LH24347 ISCW008145 ISCW002848 FBgn0234082 FBgn0221995 FBgn0206633 FBgn0188074 FBgn0173924 JGI_V11_322171 FBgn0080327 FBgn0152039 FBgn0137576 FBgn0119931 FBgn0105029 FBgn0099058 CPIJ017294 BGIBMGA001949 ACYPI008053 GB19508 AGAP006477 AAEL000128 PHUM177400 GLEAN_09105
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	CG7413 (Benos, 2000.12.13, Laviolette et al., 2005, Beall et al., 2007) dRBF (Coelho and Leevers, 2000, Secombe et al., 1997) EG:34F3.3 (Benos et al., 2001, Benos, 2000.12.13, ) FBF (Bassett et al., 1997) pRb (Szawinski et al., 2005) Rb (Tower, 2004, Campuzano, 2001, McNairn and Gilbert, 2003, Silver and Montell, 2003, Duman-Scheel et al., 2001, Du et al., 2002, Oldham et al., 2000, Steller, 2000, Weinkove and Leevers, 2000, Enikolopov et al., 1999, Kohzaki and Murakami, 2005, Gou et al., 2010) rb (Roy and Ingham, 2002) RB (Kondo and Perrimon, 2011) Rb1 (Rubin et al., 2000) Rbf (Straub and Becker, 2007, Thomas, 2005, Laviolette et al., 2005, Doggett et al., 2007, Nedelsky et al., 2010, Diangelo and Birnbaum, 2009, Axelson, 2006, Ringrose and Paro, 2007, Morris et al., 2006, Tadros et al., 2007, Ogura et al., 2009, Oktaba et al., 2008, Suzuki et al., 2009, Ahlander and Bosco, 2009, Metzendorf et al., 2009, Steele et al., 2009, Popodi et al., 2010-, Herranz et al., 2010, Herz et al., 2010, Wasbrough et al., 2010) rbf (Bjorklund et al., 2006, Moon et al., 2004, Du, 2000, Du and Dyson, 1999, Tanaka-Matakatsu et al., 2007, Steele et al., 2007, Foglietti et al., 2006, Moon et al., 2005, Hartl et al., 2007, Firth and Baker, 2005, Tanaka-Matakatsu et al., 2009, Escudero and Freeman, 2007, Brun et al., 2006, Steele et al., 2009, Martinez et al., 2009, Li et al., 2010, Buttitta et al., 2010, Nicolay et al., 2010, Acharya et al., 2010, Gehrke et al., 2010) RBF (Moon et al., 2004, Weng et al., 2003, Frolov et al., 2003, Prober and Edgar, 2001, Cayirlioglu and Duronio, 2001, Meyer et al., 2000, Kuzin et al., 2000, Prober and Edgar, 2000, Li and Vaessin, 2000, Du, 1996.3.22, Neufeld and Edgar, 1998, Duronio, 1999, Du, 1999, Secombe et al., 1998, Neufeld et al., 1998, Secombe et al., 1998, Huen et al., 1998, Neufeld et al., 1998, Wang et al., 2006, Wen et al., 2008, Steele et al., 2009, Baonza and Freeman, 2005) RbF (Saucedo et al., 2001) RBF1 (Korenjak and Brehm, 2005, Korenjak et al., 2004, Lipsick, 2004, Lewis et al., 2004, Frolov et al., 2003, Stevaux et al., 2002, Douglas et al., 2002, Douglas et al., 2000, Harbour and Dean, 2000, Herr et al., 2007, Tountas and Fortini, 2007, Georlette et al., 2007, Taylor-Harding et al., 2004, Murawska et al., 2008, Nicolay and Frolov, 2008, Wen et al., 2008, Ambrus et al., 2009, Baonza and Freeman, 2005, Lee et al., 2010) Rbf1 (Keller et al., 2005, Shibutani et al., 2005, Swanhart et al., 2004, Frolov et al., 2003, Brumby et al., 2002, Edgar et al., 2003, Key et al., 2003, Sun and Deng, 2006, Buttitta and Edgar, 2006, Shibutani et al., 2007, Buckley et al., 2007, Buckley et al., 2006, Kohzaki and Murakami, 2005, Garcia et al., 2007, Cayirlioglu et al., 2003, Shibutani et al., 2007, Davidson et al., 2008, Swanhart et al., 2007, Beall et al., 2007, Ullah et al., 2007, Lu et al., 2007, Ahlander et al., 2008, Steinhilb et al., 2007, Shibutani et al., 2008, Read et al., 2009, Khurana et al., 2006, Acharya et al., 2010, Manning et al., 2010, Hsieh et al., 2010) rbf1 (Lee and Orr-Weaver, 2003, Dimova et al., 2003, Moon et al., 2007, Ullah et al., 2007, Moon et al., 2006, Moon et al., 2008, Buttitta et al., 2007, Longworth et al., 2008, Nicholson et al., 2009, Gilbert et al., 2009)
Name Synonym	Rb (Steele et al., 2009) retinoblastoma (Axelson, 2006) Retinoblastoma (Herz et al., 2010) retinoblastoma factor (Escudero and Freeman, 2007) retinoblastoma-family homolog (Diangelo and Birnbaum, 2009) Retinoblastoma-family protein (Du and Dyson, 1996, Cho et al., 2005, Tanaka-Matakatsu et al., 2009) retinoblastoma-family protein (Martinez et al., 2009) retinoblastoma family protein 1 (Gou et al., 2010)
Secondary FlyBase IDs
References ( 199 )
Generate a list of	All references relating to this gene ( 199 )
List References by type	Research paper  ( 103 ) Supplementary material  ( 4 ) Review  ( 32 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 41 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 2 ) Curated genome annotation  ( 2 ) Protein sequence record  ( 1 ) Computer file  ( 2 ) Automatic genome annotation  ( 1 )
Recent research papers ( 15 )
	Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Nicolay et al., 2011, Genes Dev. 25(4): 323--335 Cooperation between dE2F1 and Yki/Sd defines a distinct transcriptional program necessary to bypass cell cycle exit. [FBrf0214348] Sukhanova et al., 2011, Dev. Biol. 359(2): 190--198 RBF and Rno promote photoreceptor differentiation onset through modulating EGFR signaling in the Drosophila developing eye. [FBrf0216493] Acharya et al., 2010, Mol. Biol. Cell 21(22): 3890--3901 Paradoxical Instability-Activity Relationship Defines a Novel Regulatory Pathway for Retinoblastoma Proteins. [FBrf0212298] Buttitta et al., 2010, J. Cell Biol. 189(6): 981--996 A robust cell cycle control mechanism limits E2F-induced proliferation of terminally differentiated cells in vivo. [FBrf0211029] Gehrke et al., 2010, Nature 466(7306): 637--641 Pathogenic LRRK2 negatively regulates microRNA-mediated translational repression. [FBrf0211387] Gou et al., 2010, PLoS ONE 5(5): e10581 SETDB1 Is Involved in Postembryonic DNA Methylation and Gene Silencing in Drosophila. [FBrf0210815] Herranz et al., 2010, EMBO J. 29(10): 1688--1698 The miRNA machinery targets Mei-P26 and regulates Myc protein levels in the Drosophila wing. [FBrf0210816] Herz et al., 2010, Mol. Cell. Biol. 30(10): 2485--2497 The H3K27me3 demethylase dUTX is a suppressor of notch- and Rb-dependent tumors in Drosophila. [FBrf0210661] Hsieh et al., 2010, PLoS Genet. 6(8): Tuberous Sclerosis Complex 1 Regulates dE2F1 Expression during Development and Cooperates with RBF1 to Control Proliferation and Survival. [FBrf0211700] Lee et al., 2010, Mol. Cell. Biol. 30(10): 2563--2577 Drosophila RB proteins repress differentiation-specific genes via two different mechanisms. [FBrf0210656] Li et al., 2010, Cancer Cell 17(5): 469--480 Specific Killing of Rb Mutant Cancer Cells by Inactivating TSC2. [FBrf0210758] Manning et al., 2010, Genes Dev. 24(13): 1364--1376 Loss of pRB causes centromere dysfunction and chromosomal instability. [FBrf0214901] Nedelsky et al., 2010, Neuron 67(6): 936--952 Native functions of the androgen receptor are essential to pathogenesis in a Drosophila model of spinobulbar muscular atrophy. [FBrf0211903] Nicolay et al., 2010, PLoS Genet. 6(4): e1000918 Combined inactivation of pRB and hippo pathways induces dedifferentiation in the Drosophila retina. [FBrf0210644] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...