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Gene Dmel\Rbf

General Information
Symbol	Dmel\Rbf	Species	D. melanogaster
Name	Retinoblastoma-family protein	Annotation symbol	CG7413
Feature type	protein_coding_gene	FlyBase ID	FBgn0015799
Gene Model Status	Current	Stock availability	11 publicly available
Also Known As	RBF1, Rb, EG:34F3.3, dRBF
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	1C5-1C5	Sequence location	X:691,121..695,490 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Retinoblastoma-family protein is referred to in FlyBase by the symbol Dmel\Rbf (CG7413, FBgn0015799). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; transcription factor binding; transcription regulatory region DNA binding. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; cell cycle; cell cycle process; negative regulation of cellular biosynthetic process; regulation of apoptotic process; negative regulation of metabolic process; negative regulation of biosynthetic process; regulation of cell proliferation; regulation of S phase of mitotic cell cycle; regulation of DNA binding. 62 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; organ system; external compound sense organ; thoracic segment; adult; sense organ; cell part; non-connected developing system; cell cycle; imaginal precursor. It has one annotated transcript and one annotated polypeptide. Protein features are: Cyclin-like; Domain of unknown function DUF3452, retinoblastoma-associated; Retinoblastoma-associated protein, A-box; Retinoblastoma-associated protein, B-box. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1623479_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rbf. Gene sequence location is X:691121..695490.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03	Controlled Vocabulary Terms negative regulation of G1/S transition of mitotic cell cycle
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 1C5-1C5   Limits computationally determined from genome sequence between P{EP}sdkEP369 and P{EP}EP1600&P{EP}EP1498
Experimentally Determined Cytological Location
Cytogenetic map Notes References 1C-1D   (determined by in situ hybridisation)   (Du and Dyson, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rbf for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone GH05946 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rbf-RA FBtr0070146  NM_080297   3599   845
Additional Transcript Data & Comments
Reported size (kB)	2.593 (unknown) (Dougan and DiNardo, 1992)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rbf-PA   FBpp0070141   96.8   845   6.54     NP_525036   AAF45551
Additional Polypeptide Data & Comments
Reported size (kDa)	797 (aa); 95 (kD observed) (Dougan and DiNardo, 1992)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Retinoblastoma-associated protein, B-box (IPR002719) Retinoblastoma-associated protein, A-box (IPR002720) Cyclin-like (IPR013763) Domain of unknown function DUF3452, retinoblastoma-associated (IPR024599)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202591   AA203029   AA941205   AE014298 AAF45551   AI108022   AI294097   AI513651   AI534641   AI543807   AW940215   AY047549 AAK77281   AY089532 AAL90270   BF491751   BI166421   BI167122   BI214277   BI215455   BI353656   BI363309   BI364231   BI365813   CL527993   CO268940   CO269421   CO294071   CO310357   EC082551   EC197300   EC207009   EC225098   EV586176   GH748026   GH748846   GH749802   GH751627   GH753846   GH755517   GH755799   GH755880   GH756104   GH756393   GH756703   GH757696   GH757894   GH758493   GH758749   GH759206   GH770803   GH770880   GH772236   GH772338   GH774327   GH776831   GH777168   GH777309   GH777585   GH780184   GH780531   GH781209   GH781557   GH782131   GH783420   GH783955   GH784464   GH784499   GH784785   GH785683   GH785809   GH787233   GH787360   GH787400   GH787943   GH788877   GH789575   GH789714   GH790135   GH792497   GH793060   GH794728   GH794863   GH795226   GH795282   GH796484   GH796991   GH797091   GH799520   GH799779   GH799988   GH801556   GH802176   GH802559   GH803271   GH805398   GH806011   GH806722   GH807188   GH808264   GH808697   GH810154   GH810410   GH810639   GH810665   GH811956   GH812840   GH813404   GH814688   GH814880   GH815082   GH817875   GH818819   GH818893   GH820982   GH821554   GH821749   GH822034   GH824585   GH825387   GH826687   GH827254   GH829488   GH830339   GH834855   GH835921   GH835944   GH835955   GH836208   GH836624   GH838616   GH838631   GH839694   GH843178   GH844214   GH844455   GH844468   GH845056   GH845899   GH846838   GH851399   GH853910   GH853956   GH854031   GH854173   GH854380   GH856214   GH856751   GH857462   GH859951   GH860135   GH861758   GH862205   GH862345   GH862981   GH863420   GH865597   GH867342   GH867566   GH867755   GH868639   GH869873   GH870475   GH871058   GH871316   GH871718   GH871906   GH872489   GH873225   GH874100   GH879983   GH881001   GH881358   GH881468   GH882684   GH883045   GH883794   GH884766   GH885360   GH885462   GH885798   GH887094   GH888810   GH890077   GH895451   GH895914   GH897117   GH897781   GH897952   GH899370   GH900903   GH901337   GH904442   GH908698   GH909379   GH909384   GH909945   GH911252   GH912228   GH912722   GH914206   GH915277   GH916533   GH917288   GH917353   GH917495   GH921779   GH922686   GH923206   GH926469   GH926482   GH926805   GH927100   GH927596   GH929920   GH932194   GH933329   GH933696   GH934044   GH934678   GH935325   GH937142   GH937699   GH937778   GH938474   GH939818   GH940650   X96975 CAA65661
UniProtKB/Swiss-Prot	Q24472
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 7 -- 8   antennal anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage central brain anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage dorsal head epidermis anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage visual anlage   (Fisher et al., 2012) Comment:reported as procephalic ectoderm anlage anterior endoderm anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral ectoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) Comment:reported as ventral nerve cord anlage embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium anterior endoderm   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) inclusive hindgut primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) dorsal pharyngeal muscle primordium   (Fisher et al., 2012) embryonic central brain glial cell   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) Malpighian tubule primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium visceral muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic anal pad   (Fisher et al., 2012) embryonic brain   (Fisher et al., 2012) embryonic foregut   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval midgut interstitial cell   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
chromatin inferred from direct assay (Korenjak et al., 2004) Myb complex inferred from direct assay (Lewis et al., 2004, Korenjak et al., 2004) nucleus inferred from direct assay (Acharya et al., 2010, Longworth et al., 2012) Rb-E2F complex inferred from direct assay (Du et al., 1996, Stevaux et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    158.475 Larval Midgut    57 Larval Hindgut    78.6 Larval Malpighian Tubules    28.8 Larval Fat Body    32.7 Larval Salivary Gland    136.1 Larval Trachea    91.575 Larval Carcass    82.625 Adult Head    45.1 Adult Eye    35.5 Adult Brain    107.2 Adult Thoracic-Abdominal Ganglion    61.9 Adult Crop    33.3 Adult Midgut    46.8 Adult Hindgut    40.4 Adult Malpighian Tubules    51.4 Adult Fat Body    41.3 Adult Salivary Gland    80.8 Adult Heart    30.075 Adult VirginFemale Spermatheca    33.1 Adult InseminatedFemale Spermatheca    37.9 Adult Ovary    381.2 Adult Testis    83.2 Adult Male Accessory Gland    45.2 Adult Carcass    50.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    45.1   NA  Eye    35.5   NA  Brain    107.2   158.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    61.9   NA  Crop    no informative data   57  Midgut    46.8   78.6  Hindgut    40.4   28.8  Malpighian Tubules    51.4   no informative data  Fat Body    41.3   136.1  Salivary Gland    80.8   NA  Heart    30.075   91.575  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    37.9   NA  Ovary    381.2   NA  Testis    83.2   NA  Male Accessory Gland    45.2   82.625  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    38 central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    115 ovary, mated 4-day female    112 testis, mated 4-day male    33 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (40) central nervous system, larvae L3  (38) central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female  (115) ovary, mated 4-day female  (112) testis, mated 4-day male  (33) accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    38 central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female  (115) ovary, mated 4-day female  (112) testis, mated 4-day male    33 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    38 central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    115 ovary, mated 4-day female    112 testis, mated 4-day male    33 accessory gland, mated 4-day male    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    38 central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    115 ovary, mated 4-day female    112 testis, mated 4-day male    33 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (40) central nervous system, larvae L3  (38) central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female  (115) ovary, mated 4-day female  (112) testis, mated 4-day male  (33) accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    38 central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female  115 ovary, mated 4-day female  112 testis, mated 4-day male    33 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    40 central nervous system, larvae L3    38 central nervous system, pupae P8    19 head, virgin 1-day female    10 head, virgin 4-day female    12 head, virgin 20-day female    11 head, mated 1-day female    10 head, mated 4-day female    11 head, mated 20-day female    9 head, mated 1-day male    15 head, mated 4-day male    16 head, mated 20-day male    21 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    7 digestive system, larvae L3 wandering    11 digestive system, 1-day adult    8 digestive system, 4-day adult    14 digestive system, 20-day adult    11 fat body, larvae L3 wandering    3 fat body, white prepupae    10 fat body, pupae P8    13 carcass, larvae L3 wandering    25 carcass, 1-day adult    8 carcass, 4-day adult    13 carcass, 20-day adult    12 ovary, virgin 4-day female    115 ovary, mated 4-day female    112 testis, mated 4-day male    33 accessory gland, mated 4-day male    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    110 embryo 02-04hr    24 embryo 04-06hr    48 embryo 06-08hr    52 embryo 08-10hr    36 embryo 10-12hr    28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    35 adult female 05day    59 adult female 30day    63 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (110) embryo 02-04hr    24 embryo 04-06hr  (48) embryo 06-08hr  (52) embryo 08-10hr  (36) embryo 10-12hr  (28) embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day  (35) adult female 05day  (59) adult female 30day  (63) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (110) embryo 02-04hr    24 embryo 04-06hr    48 embryo 06-08hr    52 embryo 08-10hr    36 embryo 10-12hr    28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    35 adult female 05day    59 adult female 30day    63 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    110 embryo 02-04hr    24 embryo 04-06hr    48 embryo 06-08hr    52 embryo 08-10hr    36 embryo 10-12hr    28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    35 adult female 05day    59 adult female 30day    63 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    110 embryo 02-04hr    24 embryo 04-06hr    48 embryo 06-08hr    52 embryo 08-10hr    36 embryo 10-12hr    28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    35 adult female 05day    59 adult female 30day    63 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (110) embryo 02-04hr    24 embryo 04-06hr  (48) embryo 06-08hr  (52) embryo 08-10hr  (36) embryo 10-12hr  28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day  (35) adult female 05day  (59) adult female 30day  (63) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  110 embryo 02-04hr    24 embryo 04-06hr    48 embryo 06-08hr    52 embryo 08-10hr    36 embryo 10-12hr    28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    35 adult female 05day    59 adult female 30day    63 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    110 embryo 02-04hr    24 embryo 04-06hr    48 embryo 06-08hr    52 embryo 08-10hr    36 embryo 10-12hr    28 embryo 12-14hr    19 embryo 14-16hr    19 embryo 16-18hr    17 embryo 18-20hr    10 embryo 20-22hr    8 embryo 22-24hr    8 larva L1    22 larva L2    20 larva L3 12hr old    14 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    15 larva L3 puffstage 7-9    17 white prepupae new    18 white prepupae 12hr    22 white prepupae 24hr    23 pupae 2d postWPP    13 pupae 3d postWPP    8 pupae 4d postWPP    8 adult male 01day    11 adult male 05day    13 adult male 30day    13 adult female 01day    35 adult female 05day    59 adult female 30day    63 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    66 embryonic 1182-4H    70 embryonic GM2    27 embryonic Kc167    2 embryonic S1    64 embryonic S3    39 leg disc CME L1    76 wing disc CME-W2    32 wing disc ML-DmD8    47 wing disc ML-DmD9    60 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    56 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    30 eye-antennal disc ML-DmD11    41 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    71 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    32 ovary fGS/OSS    1 ovary OSC    34 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (42) Schneider line 2 Sg4  (66) embryonic 1182-4H  (70) embryonic GM2  (27) embryonic Kc167    2 embryonic S1  (64) embryonic S3  (39) leg disc CME L1  (76) wing disc CME-W2  (32) wing disc ML-DmD8  (47) wing disc ML-DmD9  (60) wing disc ML-DmD16-c3  (34) wing disc ML-DmD21  (56) wing disc ML-DmD32  (62) haltere disc ML-DmD17-c3  (30) eye-antennal disc ML-DmD11  (41) antennal disc ML-DmD20-c5  (52) mixed discs ML-DmD4-c1  (71) CNS ML-DmBG1-c1  (30) CNS ML-DmBG2-c2  (51) tumorous blood cells mbn2  (32) ovary fGS/OSS    1 ovary OSC  (34) ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    66 embryonic 1182-4H    70 embryonic GM2    27 embryonic Kc167    2 embryonic S1    64 embryonic S3    39 leg disc CME L1    76 wing disc CME-W2    32 wing disc ML-DmD8    47 wing disc ML-DmD9    60 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    56 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    30 eye-antennal disc ML-DmD11    41 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    71 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    32 ovary fGS/OSS    1 ovary OSC    34 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    66 embryonic 1182-4H    70 embryonic GM2    27 embryonic Kc167    2 embryonic S1    64 embryonic S3    39 leg disc CME L1    76 wing disc CME-W2    32 wing disc ML-DmD8    47 wing disc ML-DmD9    60 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    56 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    30 eye-antennal disc ML-DmD11    41 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    71 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    32 ovary fGS/OSS    1 ovary OSC    34 ovary OSS    25 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    66 embryonic 1182-4H    70 embryonic GM2    27 embryonic Kc167    2 embryonic S1    64 embryonic S3    39 leg disc CME L1    76 wing disc CME-W2    32 wing disc ML-DmD8    47 wing disc ML-DmD9    60 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    56 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    30 eye-antennal disc ML-DmD11    41 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    71 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    32 ovary fGS/OSS    1 ovary OSC    34 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (42) Schneider line 2 Sg4  (66) embryonic 1182-4H  (70) embryonic GM2  27 embryonic Kc167    2 embryonic S1  (64) embryonic S3  (39) leg disc CME L1  (76) wing disc CME-W2  32 wing disc ML-DmD8  (47) wing disc ML-DmD9  (60) wing disc ML-DmD16-c3  (34) wing disc ML-DmD21  (56) wing disc ML-DmD32  (62) haltere disc ML-DmD17-c3  30 eye-antennal disc ML-DmD11  (41) antennal disc ML-DmD20-c5  (52) mixed discs ML-DmD4-c1  (71) CNS ML-DmBG1-c1  30 CNS ML-DmBG2-c2  (51) tumorous blood cells mbn2  32 ovary fGS/OSS    1 ovary OSC  (34) ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    66 embryonic 1182-4H    70 embryonic GM2    27 embryonic Kc167    2 embryonic S1    64 embryonic S3    39 leg disc CME L1    76 wing disc CME-W2    32 wing disc ML-DmD8    47 wing disc ML-DmD9    60 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    56 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    30 eye-antennal disc ML-DmD11    41 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    71 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    32 ovary fGS/OSS    1 ovary OSC    34 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    42 Schneider line 2 Sg4    66 embryonic 1182-4H    70 embryonic GM2    27 embryonic Kc167    2 embryonic S1    64 embryonic S3    39 leg disc CME L1    76 wing disc CME-W2    32 wing disc ML-DmD8    47 wing disc ML-DmD9    60 wing disc ML-DmD16-c3    34 wing disc ML-DmD21    56 wing disc ML-DmD32    62 haltere disc ML-DmD17-c3    30 eye-antennal disc ML-DmD11    41 antennal disc ML-DmD20-c5    52 mixed discs ML-DmD4-c1    71 CNS ML-DmBG1-c1    30 CNS ML-DmBG2-c2    51 tumorous blood cells mbn2    32 ovary fGS/OSS    1 ovary OSC    34 ovary OSS    25 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    34 cold shock, 4-day adult    23 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    35 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    76 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    59 Paraquat 10 mM 48 hrs, 4-day adult    53 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (34) cold shock, 4-day adult    23 heat shock, 4-day adult  (32) Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult  (35) Cadmium 100 mM 48 hrs, 4-day adult  (84) Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult  (37) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (76) Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (73) Caffeine 25 mg/ml 48 hrs, 4-day adult  (34) Paraquat 5 mM 48 hrs, 4-day adult  (59) Paraquat 10 mM 48 hrs, 4-day adult  (53) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    34 cold shock, 4-day adult    23 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    35 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    76 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    59 Paraquat 10 mM 48 hrs, 4-day adult    53 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    34 cold shock, 4-day adult    23 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    35 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    76 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    59 Paraquat 10 mM 48 hrs, 4-day adult    53 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    34 cold shock, 4-day adult    23 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    35 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    76 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    59 Paraquat 10 mM 48 hrs, 4-day adult    53 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (34) cold shock, 4-day adult    23 heat shock, 4-day adult  32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult  (35) Cadmium 100 mM 48 hrs, 4-day adult  (84) Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult  (37) Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3  (76) Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (73) Caffeine 25 mg/ml 48 hrs, 4-day adult  (34) Paraquat 5 mM 48 hrs, 4-day adult  (59) Paraquat 10 mM 48 hrs, 4-day adult  (53) Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    34 cold shock, 4-day adult    23 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    35 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    76 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    59 Paraquat 10 mM 48 hrs, 4-day adult    53 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    34 cold shock, 4-day adult    23 heat shock, 4-day adult    32 Cadmium 50 mM 6 hrs, larvae L3    12 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    35 Cadmium 100 mM 48 hrs, 4-day adult    84 Copper 0.5 mM 12 hrs, larvae L3    11 Copper 15 mM 48 hrs, 4-day adult    37 Zinc 5 mM 12 hrs, larvae L3    14 Zinc 4.5 mM 48 hrs, 4-day adult    19 Ethanol 2.5% 3 hrs, larvae L3    76 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    8 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    73 Caffeine 25 mg/ml 48 hrs, 4-day adult    34 Paraquat 5 mM 48 hrs, 4-day adult    59 Paraquat 10 mM 48 hrs, 4-day adult    53 Rotenone 2 μg 12 hrs, larvae L3    10 Rotenone 8 μg 12 hrs, larvae L3    14 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH05946 LD02906 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015799 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7413-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rbf Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4ap-md544 RbfScer\UAS.cDa lethal, with Scer\GAL4ey.PH Rbf280.Scer\UAS lethal | germline clone | rescuable maternal effect Rbfunspecified lethal | partially, with Scer\GAL4ey.PH RbfK774A.Scer\UAS RbfK774R.Scer\UAS lethal | recessive Rbf11 Rbf14 Rbf16 viable Rbf15a Rbf38a Rbf120a RbfEY07209 viable, with Scer\GAL4pnr-MD237 RbfGD4484 viable (with Rbf11) Rbf120a viable (with Rbf120a) Rbf11 Sterility Allele fertile RbfEY07209 sterile (with Rbf11) Rbf120a sterile (with Rbf120a) Rbf11 Other Phenotypes Allele cell growth defective | pupal stage | somatic clone Rbf15aΔ decreased cell number, with Scer\GAL4sca-537.4 RbfScer\UAS.cDa developmental rate defective, with Scer\GAL4hs.PH RbfScer\UAS.cDa immune response defective, with Scer\GAL4yolk RbfGD4484 increased cell death (with Rbf15aΔ) Rbf120-3-3 increased cell death (with Rbf120-3-3) Rbf15aΔ increased cell death | recessive Rbf120a increased cell death | recessive | somatic clone Rbf120a RbfΔ14 increased cell death | somatic clone Rbf14 Rbf15aΔ Rbf120a increased cell death | somatic clone | third instar larval stage Rbf15aΔ increased cell death | somatic clone | wandering third instar larval stage RbfΔ14 increased cell number | somatic clone | third instar larval stage Rbf15aΔ mitotic cell cycle defective | somatic clone, with Scer\GAL4Act5C.PP RbfScer\UAS.cDa neuroanatomy defective, with Scer\GAL4sca-537.4 RbfScer\UAS.cDa visible Rbf280.GMR RbfGMR.PD visible, with Scer\GAL4en-e16E RbfScer\UAS.cDa visible, with Scer\GAL4ey.PH Rbf1-845.Scer\UAS Rbf6KA.Scer\UAS Rbf10.Scer\UAS Rbf16.Scer\UAS Rbf223.Scer\UAS RbfK754A.Scer\UAS RbfK774A.Scer\UAS RbfK774R.Scer\UAS RbfScer\UAS.cDa visible, with Scer\GAL4GMR.PF RbfGD4484 visible, with Scer\GAL4hs.PB RbfScer\UAS.cDa visible, with Scer\GAL4pnr-MD237 RbfGD4484 visible, with Scer\GAL4vg.PM RbfScer\UAS.cDa wild-type Rbfhs.PD Phenotype manifest in Allele abdomen, with Scer\GAL4hs.PH RbfScer\UAS.cDa anterior crossvein, with Scer\GAL4dpp.blk1 RbfScer\UAS.cDa chaeta, with Scer\GAL4hs.PB RbfScer\UAS.cDa chorion (with Rbf11) Rbf120a chorion (with Rbf120a) Rbf11 cone cell | pupal stage | somatic clone RbfΔ14 embryonic/larval midgut primordium Rbf14 embryonic epidermis & S phase Rbfunspecified embryonic epidermis & S phase | germ-line clone Rbfunspecified embryonic epidermis | embryonic stage 13 Rbf14 eye Rbf14 Rbf280.GMR RbfGMR.PD eye, with Scer\GAL4ey.PH Rbf10.Scer\UAS Rbf16.Scer\UAS Rbf223.Scer\UAS RbfScer\UAS.cDa Rbf1-845.Scer\UAS RbfK774R.Scer\UAS RbfK774A.Scer\UAS Rbf6KA.Scer\UAS RbfK754A.Scer\UAS eye (with Rbf11) Rbf120a eye (with Rbf120a) Rbf11 eye | ectopic | somatic clone Rbf14 eye | somatic clone Rbf14 Rbf15aΔ eye disc Rbf120a Rbf280.GMR eye disc, with Scer\GAL4ey.PH RbfScer\UAS.cDa eye disc (with Rbf11) Rbf120a eye disc (with Rbf120a) Rbf11 eye disc | somatic clone RbfΔ14 Rbf120a eye disc | somatic clone | third instar larval stage Rbf15aΔ eye disc | third instar larval stage (with Rbf15aΔ) Rbf120-3-3 eye disc | third instar larval stage (with Rbf120-3-3) Rbf15aΔ eye disc anterior to the morphogenetic furrow | somatic clone Rbf120a eye disc posterior to the morphogenetic furrow | somatic clone | third instar larval stage Rbf15aΔ eye photoreceptor cell | pupal stage | somatic clone RbfΔ14 follicle cell (with Rbf14) Rbf120a follicle cell (with Rbf120a) Rbf14 follicle cell & cell cycle (with Rbf11) Rbf120a follicle cell & cell cycle (with Rbf120a) Rbf11 interommatidial bristle Rbf280.GMR RbfGMR.PD interommatidial bristle, with Scer\GAL4GMR.PF RbfGD4484 interommatidial bristle | somatic clone Rbf14 mesothoracic tergum, with Scer\GAL4pnr-MD237 RbfGD4484 mitotic cell cycle (with Rbf14) Rbf120a mitotic cell cycle (with Rbf120a) Rbf14 mitotic cell cycle | somatic clone, with Scer\GAL4Act5C.PP RbfScer\UAS.cDa morphogenetic furrow | somatic clone Rbf120a ommatidium Rbf14 Rbf280.GMR RbfGMR.PD ommatidium | pupal stage | somatic clone Rbf15aΔ ommatidium | somatic clone Rbf14 pigment cell RbfGMR.PD scutellar bristle Rbf14 S phase | ectopic Rbfunspecified S phase | ectopic & eye disc | posterior (with Rbf11) Rbf120a S phase | ectopic & eye disc | posterior (with Rbf120a) Rbf11 submarginal cell, with Scer\GAL4dpp.blk1 RbfScer\UAS.cDa wing | posterior compartment, with Scer\GAL4en-e16E RbfScer\UAS.cDa wing disc | somatic clone | wandering third instar larval stage RbfΔ14 wing margin, with Scer\GAL4vg.PM RbfScer\UAS.cDa
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of Rbf Class Mutagen Stocks Known lesion Rbf14 amorphic allele - genetic evidence Delta2-3 1 Yes Rbfcas-21 ethyl methanesulfonate 1 Yes Rbfd00614 1 -- RbfEY07209 P-element activity 1 -- RbfGS5173 1 -- Rbfsls-15 X ray 1 Yes Rbf11 Delta2-3 0 Yes Rbf120-3-3 0 -- Rbf120a Delta2-3 0 Yes Rbf15a Delta2-3 0 Yes Rbf15aΔ P-element activity 0 Yes Rbf16 Delta2-3 0 Yes Rbf38a Delta2-3 0 Yes RbfCAS-21 0 -- RbfGG01588 0 -- RbfSLS-15 0 Yes Rbfunspecified 0 -- RbfΔ14 loss of function allele 0 --
Alleles Carried on Transgenic Constructs ( 44 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rbf Class Mutagen Stocks Known lesion Rbft.Exel in vitro construct - genomic fragment 2 Yes RbfGD4484 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RbfGL01293 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RbfHMS03004 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Rbf1-717.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes Rbf1-845.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf10.cXa in vitro construct - amino acid replacement 0 Yes Rbf10.hs in vitro construct 0 Yes Rbf10.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf16.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf223.cXa in vitro construct - amino acid replacement 0 Yes Rbf223.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf280.cXa in vitro construct - amino acid replacement 0 Yes Rbf280.GMR in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes Rbf280.hs in vitro construct 0 Yes Rbf280.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf30.cXa in vitro construct - amino acid replacement 0 Yes Rbf30.hs in vitro construct 0 Yes Rbf30.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf555L.hs in vitro construct 0 Yes Rbf555L.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf596W.hs in vitro construct 0 Yes Rbf596W.Scer\UAS in vitro construct - regulatory fusion 0 Yes Rbf6KA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfAct5C.PD in vitro construct - regulatory fusion 0 Yes RbfcFa in vitro construct 0 Yes RbfdsRNA.cFa in vitro construct - RNAi 0 Yes RbfdsRNA.cKa in vitro construct - RNAi 0 Yes RbfdsRNA.cLa in vitro construct - RNAi 0 Yes RbfGMR.PD in vitro construct - regulatory fusion 0 Yes Rbfhs.PD in vitro construct - regulatory fusion 0 Yes Rbfie1.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RbfK754A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfK774A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfK774R.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 0 Yes RbfM4.cXa in vitro construct - amino acid replacement 0 Yes RbfN.Scer\UAS.P\T.T:Disc\RFP-mCherry,T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RbfNIG.7413R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RbfScer\UAS.cDa in vitro construct - regulatory fusion 0 Yes RbfScer\UAS.T:Zzzz\FLAG,T:Ivir\HA1 0 Yes RbfT:Zzzz\FLAG in vitro construct - regulatory fusion 0 Yes RbfWT.T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes RbfΔ728-786.Scer\UAS in vitro construct - regulatory fusion in vitro construct - deletion 0 Yes RbfΔIE.T:Zzzz\FLAG in vitro construct - deletion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)AD11 (Du and Dyson, 1999) Df(1)Exel6223 (Ryder, 2004.4.26) Df(1)su(s)83 (Du and Dyson, 1999)
Not disrupted in	Df(1)A94 (Du and Dyson, 1999) Df(1)S39 (Du and Dyson, 1999)
Duplicated in	Dp(1;3)DC012 (Popodi et al., 2010-, Venken et al., 2010) Dp(1;3)DC029 (Venken et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4484} NA P{NIG.7413R} NA P{TRiP.GL01293} NA P{TRiP.HMS03004} NA P{UASp-Rbf.N.RFP} NA P{UAS-Rbf.1-717} NA P{UAS-Rbf.1-845} NA P{UAS-Rbf.6KA} NA P{UAS-Rbf.10} NA P{UAS-Rbf.16} NA P{UAS-Rbf.30} NA P{UAS-Rbf.223} NA P{UAS-Rbf.280} NA P{UAS-Rbf.555L} NA P{UAS-Rbf.596W} NA P{UAS-Rbf.D} NA P{UAS-Rbf.FLAG-HA} NA P{UAS-Rbf.K754A} NA P{UAS-Rbf.K774A} NA P{UAS-Rbf.K774R} NA P{UAS-Rbf.Δ728-786} NA heat-shock construct P{hs-Rbf.30} NA P{hs-Rbf.280} NA P{hs-Rbf.555L} NA P{hs-Rbf.596W} NA P{hs-Rbf.D} NA P{hs-Rbf.X} NA characterization construct P{GMR-Rbf.280} NA P{GMR-Rbf} NA P{Rbf.WT.FLAG} NA P{Rbf.ΔIE.FLAG} NA P{Rbf-FRT.2.1} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}RbfEY07209 NA P{GSV2}RbfGS5173 NA P{Mae-UAS.6.11}RbfGG01588 NA P{XP}Rbfd00614 NA miscellaneous insertions P{CaSpeR}Rbf15a NA P{CaSpeR}Rbf38a NA P{CaSpeR}Rbf120a NA
Gene Ontology: Function, Process & Cellular Component ( 23 unique terms )
Terms Based on Experimental Evidence ( 18 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with Snr1 (Brumby et al., 2002) transcription factor binding inferred from physical interaction with Dp (Stevaux et al., 2002) inferred from physical interaction with Dp AND inferred from physical interaction with E2f (Du et al., 1996) inferred from physical interaction with E2f2 (Stevaux et al., 2002) inferred from physical interaction with E2f (Stevaux et al., 2002) transcription regulatory region DNA binding inferred from direct assay (Ullah et al., 2007)
Biological Process
CV term Evidence References
DNA endoreduplication inferred from mutant phenotype (Shcherbata et al., 2004) intrinsic apoptotic signaling pathway in response to DNA damage inferred from mutant phenotype (Moon et al., 2008) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) negative regulation of apoptotic process inferred from mutant phenotype (Tanaka-Matakatsu et al., 2009) negative regulation of DNA replication inferred from mutant phenotype (Bosco et al., 2001) negative regulation of G1/S transition of mitotic cell cycle inferred from mutant phenotype (Du and Dyson, 1999) negative regulation of gene expression inferred from mutant phenotype (Lu et al., 2007) negative regulation of S phase of mitotic cell cycle inferred from mutant phenotype (Firth and Baker, 2005) negative regulation of transcription from RNA polymerase II promoter inferred from direct assay (Du et al., 1996, Stevaux et al., 2002) inferred from genetic interaction with Rbf2 (Lewis et al., 2004) inferred from mutant phenotype (Korenjak et al., 2004, Du and Dyson, 1999) regulation of cell proliferation inferred from mutant phenotype (Hsieh et al., 2010) regulation of DNA binding inferred from direct assay (Du et al., 1996)
Cellular Component
CV term Evidence References
chromatin inferred from direct assay (Korenjak et al., 2004) Myb complex inferred from direct assay (Lewis et al., 2004, Korenjak et al., 2004) nucleus inferred from direct assay (Acharya et al., 2010, Longworth et al., 2012) Rb-E2F complex inferred from direct assay (Du et al., 1996, Stevaux et al., 2002)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding non-traceable author statement (Swiss-Prot Project Members, 2000.5.30)
Biological Process
CV term Evidence References
eggshell chorion gene amplification traceable author statement (McNairn and Gilbert, 2003, Lee and Orr-Weaver, 2003) endomitotic cell cycle traceable author statement (Lee and Orr-Weaver, 2003) negative regulation of cell proliferation non-traceable author statement (Potter and Xu, 2001) regulation of cell cycle inferred from sequence or structural similarity with human RBL1 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
nucleus non-traceable author statement (Swiss-Prot Project Members, 2000.5.30) Rb-E2F complex traceable author statement (Lee and Orr-Weaver, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rbf - AP-2α anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG10916 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG13321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG1416 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG15220 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG31694 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG3689 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG5642 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CG9769 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CaBP1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - CoIV anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Dp anti bait coimmunoprecipitation, western blot (Du et al., 1996) Rbf - E2f anti bait coimmunoprecipitation, western blot, two hybrid (Du et al., 1996) Rbf - E2f2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Jupiter anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - LKR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - RpS10b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Rpb8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Tm1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - Upf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - bocksbeutel anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - cpb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - l(3)72Ab anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - lin-52 anti tag coimmunoprecipitation, western blot (Lewis et al., 2012) Rbf - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - nonA-l anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - shrb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rbf - α-Spec anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rbf allele Gene References Rbf11 Cdk4 (Meyer et al., 2000) Rbf14 Cdk4 (Meyer et al., 2000) E2f (Du and Dyson, 1999, Du, 2000) Rbf120a E2f (Du and Dyson, 1999, Du, 2000) Rbf280.GMR dap (Weng et al., 2003) RbfdsRNA.cFa E2f (Frolov et al., 2003) RbfGMR.PD CycE (Secombe et al., 1998) dap (de Nooij et al., 1996, Weng et al., 2003) Nos (Kuzin et al., 2000) ptc (Duman-Scheel et al., 2002) RbfScer\UAS.cDa Cdk4 (Datar et al., 2000) CycD (Datar et al., 2000) unspecified Dp (Kuzin et al., 2000) E2f (Kuzin et al., 2000) Nsf2 (Laviolette et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57595 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015799 DroID - A comprehensive database of gene and protein interactions. • FBgn0015799 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31027
Orthologs
OrthoDB Orthologs (56) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63V0F1 Dipteran inclusive ortholog search EOG63FH52 Insect inclusive ortholog search EOG6NCK7R Arthropod inclusive ortholog search EOG60VT4N Metazoa inclusive ortholog search EOG6M6J9J
Orthologs in Drosophila Species (EOG63V0F1)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rbf     Drosophila simulans   Dsim\GD16459 Y   Drosophila sechellia   Dsec\GM19021 Y   Drosophila erecta   Dere\GG12742 Y   Drosophila yakuba   Dyak\GE16567 Y   Drosophila ananassae   Dana\GF22059 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA20332 Y   Drosophila persimilis   Dper\GL14434 Y   Drosophila willistoni   Dwil\GK19998 Y   Drosophila virilis   Dvir\GJ19490 Y   Drosophila mojavensis   Dmoj\GI14825 Y   Drosophila grimshawi   Dgri\GH12452 Y
Orthologs in non-Drosophila Dipterans (EOG63FH52)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL000128   Anopheles gambiae Malaria mosquito  Agam\AGAP006477   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017294
Orthologs in non-Dipteran Insects (EOG6NCK7R)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45686 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012064 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11911 Y Atta cephalotes Leafcutter ant  Acep\ACEP23604 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO18086 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL14724 Y Linepithema humile Argentine ant  Lhum\LH24347 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB16829 Y Solenopsis invicta Red fire ant  Sinv\SINV11565 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI008053 Y Bombyx mori Silkmoth  Bmor\BGIBMGA001949 Y Pediculus humanus Human body louse  Phum\PHUM177400 Y Tribolium castaneum Red flour beetle  Tcas\TC009105 Y
Orthologs in non-Insect Arthropods (EOG60VT4N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\COI Y Ixodes scapularis Deer tick  Isca\ISCW002848 Y Ixodes scapularis Deer tick  Isca\ISCW008145 Y
Orthologs in non-Arthropod Metazoa (EOG6M6J9J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\lin-35 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_025615gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004292gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003798gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011954gn Y Danio rerio Zebrafish  Drer\rbl1 Y Danio rerio Zebrafish  Drer\rb1 Y Danio rerio Zebrafish  Drer\ENSDARG00000045636 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000034017 Y Xenopus tropicalis Western clawed frog  Xtro\rbl2 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031493 Y Xenopus tropicalis Western clawed frog  Xtro\rbl1 Y Gallus gallus Domestic chicken  Ggal\RB1 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000003190 Y Gallus gallus Domestic chicken  Ggal\ENSGALG00000003663 Y Gallus gallus Domestic chicken  Ggal\RBL1 Y Mus musculus House mouse  Mmus\Rbl1 Y Mus musculus House mouse  Mmus\Rbl2 Y Mus musculus House mouse  Mmus\Rb1 Y Rattus norvegicus Norway rat  Rnor\Rbl1 Y Rattus norvegicus Norway rat  Rnor\Rb1 Y Rattus norvegicus Norway rat  Rnor\Rbl2 Y Homo sapiens Human  Hsap\RB1 Y Homo sapiens Human  Hsap\RBL2 Y Homo sapiens Human  Hsap\RBL1 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\RB1     Hsap\RBL2     Hsap\RBL1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16459 Drosophila sechellia Dsec\GM19021 Drosophila erecta Dere\GG12742 Drosophila yakuba Dyak\GE16567 Drosophila ananassae Dana\GF22059 Drosophila pseudoobscura pseudoobscura Dpse\GA20332 Drosophila persimilis Dper\GL14434 Drosophila willistoni Dwil\GK19998 Drosophila virilis Dvir\GJ19490 Drosophila mojavensis Dmoj\GI14825 Drosophila grimshawi Dgri\GH12452
Stocks & Reagents
Stocks Listed in FlyBase ( 11 )
Bloomington	8342 Rbfcas-21/FM7c 7435 Rbf14 w1118/FM7c 8298 y1 w1118; P{Rbf-FRT.2.1}3 8301 y1 w1118; P{Rbf-FRT.2.1}2 16803 y1 P{EPgy2}RbfEY07209 w67c23 41863 y1 sc* v1; P{TRiP.GL01293}attP40 8343 Rbfsls-15 w1118 P{neoFRT}18A/FM7c, P{ftz/lacC}YH1 36744 y1 sc* v1; P{TRiP.HMS03004}attP2/TM3, Sb1
Harvard	- P{XP}Rbfd00614
Kyoto	200757 w* P{GSV2}RbfGS5173/Binsinscy
VDRC	v10696 w1118; P{GD4484}v10696
Genomic Clones ( 1 )
	BACR19J01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 219 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH05946 LD02906
Other clones	DPiM_LD02906
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT28564 GM24583 LD03488 LD25209 LD45859 RE06540 RE07437 RE20040 RE21857 RE48083 RE49295 RE51195 SD07569 SD10719
Other clones	98413 CK01.61B.E6 CK01.122A.H11 CK01.124B.H10 Dmel_dig_000818_0651_0404 EK093132 EK094463 EK205901 EK221322 RP003007473 RP003014952 RP003016838 RP003018249 RP003020227 RP003021842 RP003032437 RP003038722 RP003038744 RP003039338 RP003040497 RP003048962 RP003053931 RP003055806 RP003063373 RP003063728 RP003064578 RP003070067 RP003071617 RP003076144 RP003081530 RP003083877 RP003086177 RP003088510 RP003091242 RP003097109 RP003099729 RP003120932 RP003121765 RP003124958 RP003125444 RP003129114 RP003133139 RP003134490 RP003138887 RP003147023 RP003151410 RP003154788 RP003157920 RP003159253 RP003160024 RP003160274 RP003160762 RP003162892 RP003165674 RP003169478 RP003171340 RP003171912 RP003180117 RP003180877 RP003192420 RP003196086 RP003196185 RP003203252 RP003203387 RP003204158 RP003204174 RP003206636 RP003207595 RP003208902 RP003217443 RP003218458 RP003219580 RP003224439 RP003226489 RP003227727 RP003230226 RP003231730 RP003233746 RP003234035 RP003240312 RP003242209 RP003242929 RP003244571 RP003247560 RP003249223 RP003251921 RP003256461 RP003260328 RP003261082 RP003262329 RP003263323 RP003266747 RP003269190 RP003277780 RP003277952 RP003280804 RP003283988 RP003284547 RP003285741 RP003285979 RP003288314 RP003292781 RP003293241 RP003294278 RP003299052 RP003306547 RP003306746 RP003307308 RP003309916 RP003310005 RP003314390 RP003315023 RP003317444 RP003319272 RP003321200 RP003333683 RP003334185 RP003340047 RP003342931 RP003361958 RP003370691 RP003389112 RP003391961 RP003393118 RP003394145 RP003396066 RP003397633 RP003401031 RP003404645 RP003408453 RP003410610 RP003413832 RP003416855 RP003416919 RP003428549 RP003432887 RP003433228 RP003437142 RP003448890 RP003452010 RP003453719 RP003458105 RP003458613 RP003458974 RP003460266 RP003461647 RP003462648 RP003462861 RP003463529 RP003472310 RP003472414 RP003474819 RP003475031 RP003476390 RP003485063 RP003491234 RP003497319 RP003501304 RP003503642 RP003504781 RP003509540 RP003512853 RP003514057 RP003517420 RP003518071 RP003521676 RP003527366 RP003530246 RP003535940 RP003548167 RP003552110 RP003552587 RP003559753 RP003563397 RP003563796 RP003564361 RP003567823 RP003568851 RP003572905 RP003574932 RP003576998 RP003581033 RP003583594 RP003583902 RP003598026 RP003598155 RP003603188 RP003603460 RP003616372 RP003623736 RP003626233 RP003629515 RP003633079 RP003644674 RP003645960 RP003651065 RP003663232 RP003666009 RP003671956 RP003672637 RP003672794 RP003678747
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0015799 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31027
Antibody Information
	polyclonal (Acharya et al., 2012, Dougan and DiNardo, 1992, Du et al., 1996)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rbf CG7413 (Nusskern, 1999.11)
Source for database merge of
Additional comments
Other Comments
	gig and Rbf synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased reactive oxygen species enzyme induction. (Li et al., 2010) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) E2f2 as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development. (Weng et al., 2003) Rbf is required at multiple stages of development. Most, if not all, of the function of Rbf during development is mediated through E2f. (Du, 2000) Rbf is dispensable for early cell cycles in the embryo, but plays an essential role in the introduction of G1 control during development. (Du and Dyson, 1999) Overexpression of dap during eye development interferes with cell cycle progression and interacts genetically with Rbf and CycE. (de Nooij et al., 1996) Identification: Yeast two hybrid screen for proteins that interact with E2f. (Du et al., 1996) Rbf plays a critical role in the regulation of cell proliferation. (Du et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_080297 RefSeq (Proteins) • NP_525036 DDBJ / EMBL / GenBank • AA202591 AAF45551 AAK77281 AAL90270 AI108022 AI294097 AL031583 AW940215 AY047549 AY089532 CAA65661 CL527993 X96975 Entrez Gene - A searchable database of RefSeq genes. • 31027 UniProtKB/Swiss-Prot • Q24472
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Retinoblastoma-associated protein, B-box (IPR002719) Retinoblastoma-associated protein, A-box (IPR002720) Cyclin-like (IPR013763) Domain of unknown function DUF3452, retinoblastoma-associated (IPR024599) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH05946 LD02906
Linkouts
	BioGRID - A database of protein and genetic interactions • 57595 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0015799 DroID - A comprehensive database of gene and protein interactions. • FBgn0015799 DRSC - Results from RNAi screens. • FBgn0015799 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0015799 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7413-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0015799 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31027 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/retnbls1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31027 modMine - Data generated by the modENCODE project. • FBgn0015799
Synonyms & Secondary IDs ( 23 )
Reported As
Symbol Synonym	CG7413 (Benos, 2000.12.13, Laviolette et al., 2005, Beall et al., 2007, Ni et al., 2011.7.19, Ni et al., 2010.12.1) dRBF (Coelho and Leevers, 2000, Secombe et al., 1997) EG:34F3.3 (Benos et al., 2001, Benos, 2000.12.13, ) FBF (Bassett et al., 1997) pRb (Szawinski et al., 2005) Rb (Tower, 2004, Campuzano, 2001, McNairn and Gilbert, 2003, Silver and Montell, 2003, Duman-Scheel et al., 2001, Du et al., 2002, Oldham et al., 2000, Steller, 2000, Weinkove and Leevers, 2000, Enikolopov et al., 1999, Kohzaki and Murakami, 2005, Gou et al., 2010) rb (Roy and Ingham, 2002) RB (Kondo and Perrimon, 2011, Korenjak et al., 2012) Rb1 (Rubin et al., 2000) Rbf (Straub and Becker, 2007, Thomas, 2005, Laviolette et al., 2005, Doggett et al., 2007, Nedelsky et al., 2010, Zhai et al., 2012, Diangelo and Birnbaum, 2009, Axelson, 2006, Ringrose and Paro, 2007, Morris et al., 2006, Jiang et al., 2009, Axelsson et al., 2011, Toku et al., 2011, Tadros et al., 2007, Ogura et al., 2009, Oktaba et al., 2008, Suzuki et al., 2009, Ahlander and Bosco, 2009, Metzendorf et al., 2009, Steele et al., 2009, Popodi et al., 2010-, Herranz et al., 2010, Herz et al., 2010, Wasbrough et al., 2010, Japanese National Institute of Genetics, 2012.5.21, Venken et al., 2010) rbf (Bjorklund et al., 2006, Moon et al., 2004, Du, 2000, Du and Dyson, 1999, Tanaka-Matakatsu et al., 2007, Steele et al., 2007, Foglietti et al., 2006, Moon et al., 2005, Hartl et al., 2007, Firth and Baker, 2005, Tanaka-Matakatsu et al., 2009, Escudero and Freeman, 2007, Brun et al., 2006, Steele et al., 2009, Martinez et al., 2009, Li et al., 2010, Buttitta et al., 2010, Nicolay et al., 2010, Acharya et al., 2010, Gehrke et al., 2010) RBF (Moon et al., 2004, Weng et al., 2003, Frolov et al., 2003, Prober and Edgar, 2001, Cayirlioglu and Duronio, 2001, Meyer et al., 2000, Kuzin et al., 2000, Prober and Edgar, 2000, Li and Vaessin, 2000, Du, 1996.3.22, Neufeld and Edgar, 1998, Duronio, 1999, Du, 1999, Secombe et al., 1998, Neufeld et al., 1998, Secombe et al., 1998, Huen et al., 1998, Neufeld et al., 1998, Wang et al., 2006, Wen et al., 2008, Milet et al., 2010, Steele et al., 2009, Baonza and Freeman, 2005) RbF (Saucedo et al., 2001) RBF1 (Korenjak and Brehm, 2005, Korenjak et al., 2004, Lipsick, 2004, Lewis et al., 2004, Frolov et al., 2003, Stevaux et al., 2002, Douglas et al., 2002, Douglas et al., 2000, Harbour and Dean, 2000, Herr et al., 2007, Tountas and Fortini, 2007, Georlette et al., 2007, Taylor-Harding et al., 2004, Murawska et al., 2008, Nicolay and Frolov, 2008, Legent et al., 2006, Wen et al., 2008, Ambrus et al., 2009, Baonza and Freeman, 2005, Lee et al., 2010, Ambrus et al., 2007, Korenjak et al., 2012) Rbf1 (Keller et al., 2005, Shibutani et al., 2005, Swanhart et al., 2004, Frolov et al., 2003, Brumby et al., 2002, Edgar et al., 2003, Key et al., 2003, Sun and Deng, 2006, Buttitta and Edgar, 2006, Shibutani et al., 2007, Buckley et al., 2007, Buckley et al., 2006, Kohzaki and Murakami, 2005, Ji et al., 2012, Garcia et al., 2007, Cayirlioglu et al., 2003, Shibutani et al., 2007, Davidson et al., 2008, Swanhart et al., 2007, Beall et al., 2007, Ullah et al., 2007, Maqbool et al., 2010, Lu et al., 2007, Ahlander et al., 2008, Steinhilb et al., 2007, Shibutani et al., 2008, Read et al., 2009, Khurana et al., 2006, Acharya et al., 2010, Manning et al., 2010, Hsieh et al., 2010, Stevaux et al., 2005, Datar et al., 2006, Miles et al., 2012) rbf1 (Lee and Orr-Weaver, 2003, Dimova et al., 2003, Moon et al., 2007, Ullah et al., 2007, Moon et al., 2006, Moon et al., 2008, Buttitta et al., 2007, Longworth et al., 2008, Nicholson et al., 2009, Gilbert et al., 2009, Longworth et al., 2012, Korenjak et al., 2012, Popova et al., 2011)
Name Synonym	Rb (Steele et al., 2009) retinoblastoma (Axelson, 2006) Retinoblastoma (Herz et al., 2010) retinoblastoma factor (Escudero and Freeman, 2007) retinoblastoma-family homolog (Diangelo and Birnbaum, 2009) Retinoblastoma-family protein (Du and Dyson, 1996, Cho et al., 2005, Tanaka-Matakatsu et al., 2009) retinoblastoma-family protein (Martinez et al., 2009) retinoblastoma family protein 1 (Gou et al., 2010)
Secondary FlyBase IDs
References ( 229 )
Generate a list of	All references relating to this gene ( 229 )
List References by type	Research paper  ( 127 ) Supplementary material  ( 5 ) Review  ( 32 ) Personal communication to FlyBase  ( 12 ) Abstract  ( 41 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Conference report  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Protein sequence record  ( 1 ) Website  ( 1 )
Recent research papers ( 19 )
	Acharya et al., 2012, G3 (Bethesda) 2(11): 1459--1472 Evidence for autoregulation and cell signaling pathway regulation from genome-wide binding of the Drosophila retinoblastoma protein. [FBrf0220009] Adamson and Lajeunesse, 2012, ScientificWorldJournal 2012: 347597 A Study of Epstein-Barr Virus BRLF1 Activity in a Drosophila Model System. [FBrf0218446] Bayarmagnai et al., 2012, Cell Cycle 11(22): 4191--4202 Drosophila GAGA factor is required for full activation of the dE2f1-Yki/Sd transcriptional program. [FBrf0220001] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387 RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Lee et al., 2012, Mol. Cell. Biol. 32(11): 2110--2120 A Dual Role for the dREAM/MMB Complex in the Regulation of Differentiation-Specific E2F/RB Target Genes. [FBrf0218296] Lewis et al., 2012, Mol. Cell. Biol. 32(16): 3218--3227 Drosophila Lin-52 Acts in Opposition to Repressive Components of the Myb-MuvB/dREAM Complex. [FBrf0219063] Longworth et al., 2012, PLoS Genet. 8(4): e1002618 A Shared Role for RBF1 and dCAP-D3 in the Regulation of Transcription with Consequences for Innate Immunity. [FBrf0218058] Miles et al., 2012, Genes Dev. 26(4): 356--368 Pumilio facilitates miRNA regulation of the E2F3 oncogene. [FBrf0217481] Palgi et al., 2012, BMC Genomics 13: 134 Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes. [FBrf0218515] Raj et al., 2012, Cell Cycle 11(20): 3731--3738 Rbf1 degron dysfunction enhances cellular DNA replication. [FBrf0219758] Raj et al., 2012, J. Biol. Chem. 287(50): 41835--41843 Ubiquitination of Retinoblastoma Family Protein 1 Potentiates Gene-specific Repression Function. [FBrf0220180] Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Axelsson et al., 2011, BMC Bioinformatics 12: 342 Extracting quantitative genetic interaction phenotypes from matrix combinatorial RNAi. [FBrf0215222] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Nicolay et al., 2011, Genes Dev. 25(4): 323--335 Cooperation between dE2F1 and Yki/Sd defines a distinct transcriptional program necessary to bypass cell cycle exit. [FBrf0214348] Popova et al., 2011, J. Cell Sci. 124(24): 4203--4212 Rb deficiency during Drosophila eye development deregulates EMC, causing defects in the development of photoreceptors and cone cells. [FBrf0217175] Sukhanova et al., 2011, Dev. Biol. 359(2): 190--198 RBF and Rno promote photoreceptor differentiation onset through modulating EGFR signaling in the Drosophila developing eye. [FBrf0216493] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...