General Information Symbol Dmel\ RbfSpecies D. melanogaster Name Retinoblastoma-family protein Annotation symbol CG7413 Feature type protein_coding_gene FlyBase ID FBgn0015799 Gene Model Status Current Stock availability 11 publicly
available Also Known As RBF1, Rb, EG:34F3.3, dRBF Genomic Location Chromosome (arm) X Recombination map Cytogenetic map 1C5-1C5 Sequence location X:691,121..695,490 [-]
Genomic Maps
Summary Information Automatically generated summary
See sections below for more information The gene
Retinoblastoma-family protein is referred to in FlyBase by the symbol
Dmel\Rbf (CG7413, FBgn0015799). It is a protein_coding_gene from
Drosophila melanogaster . There is experimental evidence that it has the
molecular function : protein binding; transcription factor binding; transcription regulatory region DNA binding. There is experimental evidence for 12 unique
biological process terms, many of which group under: biological regulation; cell cycle; cell cycle process; negative regulation of cellular biosynthetic process; regulation of apoptotic process; negative regulation of metabolic process; negative regulation of biosynthetic process; regulation of cell proliferation; regulation of S phase of mitotic cell cycle; regulation of DNA binding.
62 alleles are reported . The
phenotypes of these alleles are annotated with: organ system subdivision; adult segment; organ system; external compound sense organ; thoracic segment; adult; sense organ; cell part; non-connected developing system; cell cycle; imaginal precursor. It has
one annotated transcript and
one annotated polypeptide .
Protein features are: Cyclin-like; Domain of unknown function DUF3452, retinoblastoma-associated; Retinoblastoma-associated protein, A-box; Retinoblastoma-associated protein, B-box. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1623479_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rbf. Gene sequence location is
X:691121..695490 .
User Contributed Data External Summaries
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FB2013_03
Controlled Vocabulary Terms
FB2013_02
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Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
1C-1D
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes Gene Model & Products Comments on Gene
Model DGC clone GH05946 appears problematic: incomplete CDS
Transcript Data Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences
Polypeptide Data Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) 797 (aa); 95 (kD observed)
Comments External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. External Data Linkouts Crossreferences Expression Data Transcript ExpressionComment: maternally deposited
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as ventral nerve cord anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as muscle system primordium
Comment: reported as muscle system primordium
Additional Descriptive Data
Marker for Subcellular Localization CV Term Polypeptide Expression
Additional Descriptive Data
Marker for Subcellular Localization (GO Cellular Component) inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference
FlyAtlas Anatomy Microarray
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
High
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
158.475
Larval Midgut
57
Larval Hindgut
78.6
Larval Malpighian Tubules
28.8
Larval Fat Body
32.7
Larval Salivary Gland
136.1
Larval Trachea
91.575
Larval Carcass
82.625
Adult Head
45.1
Adult Eye
35.5
Adult Brain
107.2
Adult Thoracic-Abdominal Ganglion
61.9
Adult Crop
33.3
Adult Midgut
46.8
Adult Hindgut
40.4
Adult Malpighian Tubules
51.4
Adult Fat Body
41.3
Adult Salivary Gland
80.8
Adult Heart
30.075
Adult VirginFemale Spermatheca
33.1
Adult InseminatedFemale Spermatheca
37.9
Adult Ovary
381.2
Adult Testis
83.2
Adult Male Accessory Gland
45.2
Adult Carcass
50.5
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA
Head
45.1
NA
Eye
35.5
NA
Brain
107.2
158.475
Central Nervous System
NA
NA
Thoracic-Abdominal Ganglion
61.9
NA
Crop
no informative data
57
Midgut
46.8
78.6
Hindgut
40.4
28.8
Malpighian Tubules
51.4
no informative data
Fat Body
41.3
136.1
Salivary Gland
80.8
NA
Heart
30.075
91.575
Trachea
NA
NA
VirginFemale Spermatheca
no informative data
NA
InseminatedFemale Spermatheca
37.9
NA
Ovary
381.2
NA
Testis
83.2
NA
Male Accessory Gland
45.2
82.625
Carcass
no informative data
modENCODE Anatomy RNA-Seq
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
40
central nervous system, larvae L3
38
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
115
ovary, mated 4-day female
112
testis, mated 4-day male
33
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(40)
central nervous system, larvae L3
(38)
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
(115)
ovary, mated 4-day female
(112)
testis, mated 4-day male
(33)
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
40
central nervous system, larvae L3
38
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
(115)
ovary, mated 4-day female
(112)
testis, mated 4-day male
33
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
40
central nervous system, larvae L3
38
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
115
ovary, mated 4-day female
112
testis, mated 4-day male
33
accessory gland, mated 4-day male
9
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
40
central nervous system, larvae L3
38
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
115
ovary, mated 4-day female
112
testis, mated 4-day male
33
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(40)
central nervous system, larvae L3
(38)
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
(115)
ovary, mated 4-day female
(112)
testis, mated 4-day male
(33)
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
40
central nervous system, larvae L3
38
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
115
ovary, mated 4-day female
112
testis, mated 4-day male
33
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
40
central nervous system, larvae L3
38
central nervous system, pupae P8
19
head, virgin 1-day female
10
head, virgin 4-day female
12
head, virgin 20-day female
11
head, mated 1-day female
10
head, mated 4-day female
11
head, mated 20-day female
9
head, mated 1-day male
15
head, mated 4-day male
16
head, mated 20-day male
21
salivary gland, larvae L3 wandering
2
salivary gland, white prepupae
7
digestive system, larvae L3 wandering
11
digestive system, 1-day adult
8
digestive system, 4-day adult
14
digestive system, 20-day adult
11
fat body, larvae L3 wandering
3
fat body, white prepupae
10
fat body, pupae P8
13
carcass, larvae L3 wandering
25
carcass, 1-day adult
8
carcass, 4-day adult
13
carcass, 20-day adult
12
ovary, virgin 4-day female
115
ovary, mated 4-day female
112
testis, mated 4-day male
33
accessory gland, mated 4-day male
9
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
modENCODE Development RNA-Seq
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
110
embryo 02-04hr
24
embryo 04-06hr
48
embryo 06-08hr
52
embryo 08-10hr
36
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
35
adult female 05day
59
adult female 30day
63
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(110)
embryo 02-04hr
24
embryo 04-06hr
(48)
embryo 06-08hr
(52)
embryo 08-10hr
(36)
embryo 10-12hr
(28)
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
(35)
adult female 05day
(59)
adult female 30day
(63)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
(110)
embryo 02-04hr
24
embryo 04-06hr
48
embryo 06-08hr
52
embryo 08-10hr
36
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
35
adult female 05day
59
adult female 30day
63
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
110
embryo 02-04hr
24
embryo 04-06hr
48
embryo 06-08hr
52
embryo 08-10hr
36
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
35
adult female 05day
59
adult female 30day
63
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
110
embryo 02-04hr
24
embryo 04-06hr
48
embryo 06-08hr
52
embryo 08-10hr
36
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
35
adult female 05day
59
adult female 30day
63
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(110)
embryo 02-04hr
24
embryo 04-06hr
(48)
embryo 06-08hr
(52)
embryo 08-10hr
(36)
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
(35)
adult female 05day
(59)
adult female 30day
(63)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
110
embryo 02-04hr
24
embryo 04-06hr
48
embryo 06-08hr
52
embryo 08-10hr
36
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
35
adult female 05day
59
adult female 30day
63
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
110
embryo 02-04hr
24
embryo 04-06hr
48
embryo 06-08hr
52
embryo 08-10hr
36
embryo 10-12hr
28
embryo 12-14hr
19
embryo 14-16hr
19
embryo 16-18hr
17
embryo 18-20hr
10
embryo 20-22hr
8
embryo 22-24hr
8
larva L1
22
larva L2
20
larva L3 12hr old
14
larva L3 puffstage 1-2
10
larva L3 puffstage 3-6
15
larva L3 puffstage 7-9
17
white prepupae new
18
white prepupae 12hr
22
white prepupae 24hr
23
pupae 2d postWPP
13
pupae 3d postWPP
8
pupae 4d postWPP
8
adult male 01day
11
adult male 05day
13
adult male 30day
13
adult female 01day
35
adult female 05day
59
adult female 30day
63
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
modENCODE Cell Lines RNA-Seq
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
42
Schneider line 2 Sg4
66
embryonic 1182-4H
70
embryonic GM2
27
embryonic Kc167
2
embryonic S1
64
embryonic S3
39
leg disc CME L1
76
wing disc CME-W2
32
wing disc ML-DmD8
47
wing disc ML-DmD9
60
wing disc ML-DmD16-c3
34
wing disc ML-DmD21
56
wing disc ML-DmD32
62
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
41
antennal disc ML-DmD20-c5
52
mixed discs ML-DmD4-c1
71
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
51
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
34
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(42)
Schneider line 2 Sg4
(66)
embryonic 1182-4H
(70)
embryonic GM2
(27)
embryonic Kc167
2
embryonic S1
(64)
embryonic S3
(39)
leg disc CME L1
(76)
wing disc CME-W2
(32)
wing disc ML-DmD8
(47)
wing disc ML-DmD9
(60)
wing disc ML-DmD16-c3
(34)
wing disc ML-DmD21
(56)
wing disc ML-DmD32
(62)
haltere disc ML-DmD17-c3
(30)
eye-antennal disc ML-DmD11
(41)
antennal disc ML-DmD20-c5
(52)
mixed discs ML-DmD4-c1
(71)
CNS ML-DmBG1-c1
(30)
CNS ML-DmBG2-c2
(51)
tumorous blood cells mbn2
(32)
ovary fGS/OSS
1
ovary OSC
(34)
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
42
Schneider line 2 Sg4
66
embryonic 1182-4H
70
embryonic GM2
27
embryonic Kc167
2
embryonic S1
64
embryonic S3
39
leg disc CME L1
76
wing disc CME-W2
32
wing disc ML-DmD8
47
wing disc ML-DmD9
60
wing disc ML-DmD16-c3
34
wing disc ML-DmD21
56
wing disc ML-DmD32
62
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
41
antennal disc ML-DmD20-c5
52
mixed discs ML-DmD4-c1
71
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
51
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
34
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
42
Schneider line 2 Sg4
66
embryonic 1182-4H
70
embryonic GM2
27
embryonic Kc167
2
embryonic S1
64
embryonic S3
39
leg disc CME L1
76
wing disc CME-W2
32
wing disc ML-DmD8
47
wing disc ML-DmD9
60
wing disc ML-DmD16-c3
34
wing disc ML-DmD21
56
wing disc ML-DmD32
62
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
41
antennal disc ML-DmD20-c5
52
mixed discs ML-DmD4-c1
71
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
51
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
34
ovary OSS
25
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
42
Schneider line 2 Sg4
66
embryonic 1182-4H
70
embryonic GM2
27
embryonic Kc167
2
embryonic S1
64
embryonic S3
39
leg disc CME L1
76
wing disc CME-W2
32
wing disc ML-DmD8
47
wing disc ML-DmD9
60
wing disc ML-DmD16-c3
34
wing disc ML-DmD21
56
wing disc ML-DmD32
62
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
41
antennal disc ML-DmD20-c5
52
mixed discs ML-DmD4-c1
71
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
51
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
34
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(42)
Schneider line 2 Sg4
(66)
embryonic 1182-4H
(70)
embryonic GM2
27
embryonic Kc167
2
embryonic S1
(64)
embryonic S3
(39)
leg disc CME L1
(76)
wing disc CME-W2
32
wing disc ML-DmD8
(47)
wing disc ML-DmD9
(60)
wing disc ML-DmD16-c3
(34)
wing disc ML-DmD21
(56)
wing disc ML-DmD32
(62)
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
(41)
antennal disc ML-DmD20-c5
(52)
mixed discs ML-DmD4-c1
(71)
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
(51)
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
(34)
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
42
Schneider line 2 Sg4
66
embryonic 1182-4H
70
embryonic GM2
27
embryonic Kc167
2
embryonic S1
64
embryonic S3
39
leg disc CME L1
76
wing disc CME-W2
32
wing disc ML-DmD8
47
wing disc ML-DmD9
60
wing disc ML-DmD16-c3
34
wing disc ML-DmD21
56
wing disc ML-DmD32
62
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
41
antennal disc ML-DmD20-c5
52
mixed discs ML-DmD4-c1
71
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
51
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
34
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
42
Schneider line 2 Sg4
66
embryonic 1182-4H
70
embryonic GM2
27
embryonic Kc167
2
embryonic S1
64
embryonic S3
39
leg disc CME L1
76
wing disc CME-W2
32
wing disc ML-DmD8
47
wing disc ML-DmD9
60
wing disc ML-DmD16-c3
34
wing disc ML-DmD21
56
wing disc ML-DmD32
62
haltere disc ML-DmD17-c3
30
eye-antennal disc ML-DmD11
41
antennal disc ML-DmD20-c5
52
mixed discs ML-DmD4-c1
71
CNS ML-DmBG1-c1
30
CNS ML-DmBG2-c2
51
tumorous blood cells mbn2
32
ovary fGS/OSS
1
ovary OSC
34
ovary OSS
25
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
modENCODE Treatments RNA-Seq
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
34
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
35
Cadmium 100 mM 48 hrs, 4-day adult
84
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
37
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
76
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
73
Caffeine 25 mg/ml 48 hrs, 4-day adult
34
Paraquat 5 mM 48 hrs, 4-day adult
59
Paraquat 10 mM 48 hrs, 4-day adult
53
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
(34)
cold shock, 4-day adult
23
heat shock, 4-day adult
(32)
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
(35)
Cadmium 100 mM 48 hrs, 4-day adult
(84)
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
(37)
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
(76)
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(73)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(34)
Paraquat 5 mM 48 hrs, 4-day adult
(59)
Paraquat 10 mM 48 hrs, 4-day adult
(53)
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
34
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
35
Cadmium 100 mM 48 hrs, 4-day adult
84
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
37
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
76
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
73
Caffeine 25 mg/ml 48 hrs, 4-day adult
34
Paraquat 5 mM 48 hrs, 4-day adult
59
Paraquat 10 mM 48 hrs, 4-day adult
53
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
34
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
35
Cadmium 100 mM 48 hrs, 4-day adult
84
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
37
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
76
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
73
Caffeine 25 mg/ml 48 hrs, 4-day adult
34
Paraquat 5 mM 48 hrs, 4-day adult
59
Paraquat 10 mM 48 hrs, 4-day adult
53
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
34
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
35
Cadmium 100 mM 48 hrs, 4-day adult
84
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
37
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
76
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
73
Caffeine 25 mg/ml 48 hrs, 4-day adult
34
Paraquat 5 mM 48 hrs, 4-day adult
59
Paraquat 10 mM 48 hrs, 4-day adult
53
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
(34)
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
(35)
Cadmium 100 mM 48 hrs, 4-day adult
(84)
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
(37)
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
(76)
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(73)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(34)
Paraquat 5 mM 48 hrs, 4-day adult
(59)
Paraquat 10 mM 48 hrs, 4-day adult
(53)
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
34
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
35
Cadmium 100 mM 48 hrs, 4-day adult
84
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
37
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
76
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
73
Caffeine 25 mg/ml 48 hrs, 4-day adult
34
Paraquat 5 mM 48 hrs, 4-day adult
59
Paraquat 10 mM 48 hrs, 4-day adult
53
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
34
cold shock, 4-day adult
23
heat shock, 4-day adult
32
Cadmium 50 mM 6 hrs, larvae L3
12
Cadmium 50 mM 12 hrs, larvae L3
9
Cadmium 50 mM 48 hrs, 4-day adult
35
Cadmium 100 mM 48 hrs, 4-day adult
84
Copper 0.5 mM 12 hrs, larvae L3
11
Copper 15 mM 48 hrs, 4-day adult
37
Zinc 5 mM 12 hrs, larvae L3
14
Zinc 4.5 mM 48 hrs, 4-day adult
19
Ethanol 2.5% 3 hrs, larvae L3
76
Ethanol 5% 3 hrs, larvae L3
9
Ethanol 10% 3 hrs, larvae L3
12
Caffeine 1.5 mg/ml 4 hrs, larvae L3
8
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
73
Caffeine 25 mg/ml 48 hrs, 4-day adult
34
Paraquat 5 mM 48 hrs, 4-day adult
59
Paraquat 10 mM 48 hrs, 4-day adult
53
Rotenone 2 μg 12 hrs, larvae L3
10
Rotenone 8 μg 12 hrs, larvae L3
14
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
A cluster of genes with similar mRNA expression dynamics across development.
External Data & Images
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
Alleles & Phenotypes Summary of Allele Phenotypes embryonic epidermis & S phase
embryonic epidermis & S phase | germ-line clone
follicle cell & cell cycle (with
Rbf11 )
follicle cell & cell cycle (with
Rbf120a )
S phase | ectopic & eye disc | posterior (with
Rbf11 )
S phase | ectopic & eye disc | posterior (with
Rbf120a )
Classical Alleles ( 18 ) For All Classical Alleles Show Alleles Carried on Transgenic Constructs
( 44 ) For All Alleles Carried on Transgenic
Constructs Show Aneuploid Aberrations Disrupted in Not disrupted in Duplicated in Transgenic Constructs & Insertions
Transgenic Constructs UAS construct
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
NA
characterization construct
Insertions insertion of mobile activating element
Gene Ontology: Function, Process
& Cellular Component ( 23 unique terms ) Terms Based on
Experimental Evidence ( 18 terms ) Molecular Function inferred from physical interaction with
Snr1 inferred from physical interaction with
Dp inferred from physical interaction with
Dp AND inferred from physical interaction with
E2f inferred from physical interaction with
E2f2 inferred from physical interaction with
E2f inferred from direct assay
Biological Process inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with
nbs inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
inferred from genetic interaction with
Rbf2 inferred from mutant phenotype
inferred from mutant phenotype
inferred from direct assay
Cellular Component inferred from direct assay
inferred from direct assay
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or
Assertions ( 7 terms ) Molecular Function non-traceable author statement
Biological Process traceable author statement
traceable author statement
non-traceable author statement
inferred from sequence or structural similarity with
human RBL1 Cellular Component non-traceable author statement
traceable author statement
Sequence Ontology: Class of Gene Interactions & Pathways
Summary of Physical Interactions
protein-protein anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti bait coimmunoprecipitation, western blot
anti bait coimmunoprecipitation, western blot, two hybrid
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, western blot
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
anti tag coimmunoprecipitation, peptide massfingerprinting
Summary of Genetic Interactions Interacts with Please look at the allele data
for full details of the genetic interactions
External Data Linkouts
- A database of protein and genetic interactions
- DPiM, Drosophila Protein interaction Map
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
Orthologs
OrthoDB Orthologs (56)
- based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
Orthologs in Drosophila Species
(EOG63V0F1 )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
Orthologs in non-Drosophila Dipterans
(EOG63FH52 )
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects
(EOG6NCK7R )
Apis mellifera
Western honey bee
Amel\GB45686
Y
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG012064
Y
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH11911
Y
Atta cephalotes
Leafcutter ant
Acep\ACEP23604
Y
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO18086
Y
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL14724
Y
Linepithema humile
Argentine ant
Lhum\LH24347
Y
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB16829
Y
Solenopsis invicta
Red fire ant
Sinv\SINV11565
Y
Acyrthosiphon pisum
Pea aphid
Y
Pediculus humanus
Human body louse
Y
Tribolium castaneum
Red flour beetle
Y
Orthologs in non-Insect Arthropods
(EOG60VT4N )
Daphnia pulex
Water flea
Y
Ixodes scapularis
Deer tick
Y
Ixodes scapularis
Deer tick
Y
Orthologs in non-Arthropod Metazoa
(EOG6M6J9J )
Caenorhabditis elegans
Nematode
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Strongylocentrotus purpuratus
Purple sea urchin
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Xenopus tropicalis
Western clawed frog
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Gallus gallus
Domestic chicken
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Mus musculus
House mouse
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Rattus norvegicus
Norway rat
Y
Human Orthologs (3)AAA Orthologs (11)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Stocks & Reagents Stocks Listed in FlyBase ( 11 )Bloomington Harvard Kyoto VDRC Genomic Clones ( 1 )Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
cDNA Clones ( 219 )Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones RNAi & Array Information Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Other Information Discoverer Etymology Identification Relationship to Other Genes Source for database identity of Source for identity of: Rbf CG7413
Source for database merge of Additional comments Other Comments gig and
Rbf synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased reactive oxygen species enzyme induction.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
E2f2 as part of the E2f-Rb complexes has a role in the down regulation of
CycE during the gap phase of endocycling cells in normal development.
Rbf is required at multiple stages of development. Most, if not all, of the function of
Rbf during development is mediated through
E2f .
Rbf is dispensable for early cell cycles in the embryo, but plays an essential role in the introduction of G
1 control during development.
Overexpression of
dap during eye development interferes with cell cycle progression and interacts genetically with
Rbf and
CycE .
Identification: Yeast two hybrid screen for proteins that interact with
E2f .
Rbf plays a critical role in the regulation of cell proliferation.
External Crossreferences & Linkouts
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
- Patterns of gene expression in Drosophila embryogenesis
Linkouts
- A database of protein and genetic interactions
- DPiM, Drosophila Protein interaction Map
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
- A cyberspace guide to Drosophila development and metazoan evolution
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
Synonyms & Secondary IDs
( 23 )Reported As Symbol Synonym Rb
(
Tower, 2004 ,
Campuzano, 2001 ,
McNairn and Gilbert, 2003 ,
Silver and Montell, 2003 ,
Duman-Scheel et al., 2001 ,
Du et al., 2002 ,
Oldham et al., 2000 ,
Steller, 2000 ,
Weinkove and Leevers, 2000 ,
Enikolopov et al., 1999 ,
Kohzaki and Murakami, 2005 ,
Gou et al., 2010 )
Rbf
(
Straub and Becker, 2007 ,
Thomas, 2005 ,
Laviolette et al., 2005 ,
Doggett et al., 2007 ,
Nedelsky et al., 2010 ,
Zhai et al., 2012 ,
Diangelo and Birnbaum, 2009 ,
Axelson, 2006 ,
Ringrose and Paro, 2007 ,
Morris et al., 2006 ,
Jiang et al., 2009 ,
Axelsson et al., 2011 ,
Toku et al., 2011 ,
Tadros et al., 2007 ,
Ogura et al., 2009 ,
Oktaba et al., 2008 ,
Suzuki et al., 2009 ,
Ahlander and Bosco, 2009 ,
Metzendorf et al., 2009 ,
Steele et al., 2009 ,
Popodi et al., 2010- ,
Herranz et al., 2010 ,
Herz et al., 2010 ,
Wasbrough et al., 2010 ,
Japanese National Institute of Genetics, 2012.5.21 ,
Venken et al., 2010 )
rbf
(
Bjorklund et al., 2006 ,
Moon et al., 2004 ,
Du, 2000 ,
Du and Dyson, 1999 ,
Tanaka-Matakatsu et al., 2007 ,
Steele et al., 2007 ,
Foglietti et al., 2006 ,
Moon et al., 2005 ,
Hartl et al., 2007 ,
Firth and Baker, 2005 ,
Tanaka-Matakatsu et al., 2009 ,
Escudero and Freeman, 2007 ,
Brun et al., 2006 ,
Steele et al., 2009 ,
Martinez et al., 2009 ,
Li et al., 2010 ,
Buttitta et al., 2010 ,
Nicolay et al., 2010 ,
Acharya et al., 2010 ,
Gehrke et al., 2010 )
RBF
(
Moon et al., 2004 ,
Weng et al., 2003 ,
Frolov et al., 2003 ,
Prober and Edgar, 2001 ,
Cayirlioglu and Duronio, 2001 ,
Meyer et al., 2000 ,
Kuzin et al., 2000 ,
Prober and Edgar, 2000 ,
Li and Vaessin, 2000 ,
Du, 1996.3.22 ,
Neufeld and Edgar, 1998 ,
Duronio, 1999 ,
Du, 1999 ,
Secombe et al., 1998 ,
Neufeld et al., 1998 ,
Secombe et al., 1998 ,
Huen et al., 1998 ,
Neufeld et al., 1998 ,
Wang et al., 2006 ,
Wen et al., 2008 ,
Milet et al., 2010 ,
Steele et al., 2009 ,
Baonza and Freeman, 2005 )
RBF1
(
Korenjak and Brehm, 2005 ,
Korenjak et al., 2004 ,
Lipsick, 2004 ,
Lewis et al., 2004 ,
Frolov et al., 2003 ,
Stevaux et al., 2002 ,
Douglas et al., 2002 ,
Douglas et al., 2000 ,
Harbour and Dean, 2000 ,
Herr et al., 2007 ,
Tountas and Fortini, 2007 ,
Georlette et al., 2007 ,
Taylor-Harding et al., 2004 ,
Murawska et al., 2008 ,
Nicolay and Frolov, 2008 ,
Legent et al., 2006 ,
Wen et al., 2008 ,
Ambrus et al., 2009 ,
Baonza and Freeman, 2005 ,
Lee et al., 2010 ,
Ambrus et al., 2007 ,
Korenjak et al., 2012 )
Rbf1
(
Keller et al., 2005 ,
Shibutani et al., 2005 ,
Swanhart et al., 2004 ,
Frolov et al., 2003 ,
Brumby et al., 2002 ,
Edgar et al., 2003 ,
Key et al., 2003 ,
Sun and Deng, 2006 ,
Buttitta and Edgar, 2006 ,
Shibutani et al., 2007 ,
Buckley et al., 2007 ,
Buckley et al., 2006 ,
Kohzaki and Murakami, 2005 ,
Ji et al., 2012 ,
Garcia et al., 2007 ,
Cayirlioglu et al., 2003 ,
Shibutani et al., 2007 ,
Davidson et al., 2008 ,
Swanhart et al., 2007 ,
Beall et al., 2007 ,
Ullah et al., 2007 ,
Maqbool et al., 2010 ,
Lu et al., 2007 ,
Ahlander et al., 2008 ,
Steinhilb et al., 2007 ,
Shibutani et al., 2008 ,
Read et al., 2009 ,
Khurana et al., 2006 ,
Acharya et al., 2010 ,
Manning et al., 2010 ,
Hsieh et al., 2010 ,
Stevaux et al., 2005 ,
Datar et al., 2006 ,
Miles et al., 2012 )
rbf1
(
Lee and Orr-Weaver, 2003 ,
Dimova et al., 2003 ,
Moon et al., 2007 ,
Ullah et al., 2007 ,
Moon et al., 2006 ,
Moon et al., 2008 ,
Buttitta et al., 2007 ,
Longworth et al., 2008 ,
Nicholson et al., 2009 ,
Gilbert et al., 2009 ,
Longworth et al., 2012 ,
Korenjak et al., 2012 ,
Popova et al., 2011 )
Name Synonym retinoblastoma-family homolog
Retinoblastoma-family protein
retinoblastoma-family protein
retinoblastoma family protein 1
Secondary FlyBase
IDs References ( 229 )Generate a list of List References by type
Recent research papers ( 19 )
Acharya et al., 2012, G3 (Bethesda) 2(11): 1459--1472Evidence for autoregulation and cell signaling pathway regulation from genome-wide binding of the Drosophila retinoblastoma protein. [FBrf0220009] Adamson and Lajeunesse, 2012, ScientificWorldJournal 2012: 347597A Study of Epstein-Barr Virus BRLF1 Activity in a Drosophila Model System. [FBrf0218446] Bayarmagnai et al., 2012, Cell Cycle 11(22): 4191--4202Drosophila GAGA factor is required for full activation of the dE2f1-Yki/Sd transcriptional program. [FBrf0220001] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Korenjak et al., 2012, Mol. Cell. Biol. 32(21): 4375--4387RBF Binding to both Canonical E2F Targets and Noncanonical Targets Depends on Functional dE2F/dDP Complexes. [FBrf0219643] Lee et al., 2012, Mol. Cell. Biol. 32(11): 2110--2120A Dual Role for the dREAM/MMB Complex in the Regulation of Differentiation-Specific E2F/RB Target Genes. [FBrf0218296] Lewis et al., 2012, Mol. Cell. Biol. 32(16): 3218--3227Drosophila Lin-52 Acts in Opposition to Repressive Components of the Myb-MuvB/dREAM Complex. [FBrf0219063] Longworth et al., 2012, PLoS Genet. 8(4): e1002618A Shared Role for RBF1 and dCAP-D3 in the Regulation of Transcription with Consequences for Innate Immunity. [FBrf0218058] Miles et al., 2012, Genes Dev. 26(4): 356--368Pumilio facilitates miRNA regulation of the E2F3 oncogene. [FBrf0217481] Palgi et al., 2012, BMC Genomics 13: 134Gene expression analysis of Drosophilaa Manf mutants reveals perturbations in membrane traffic and major metabolic changes. [FBrf0218515] Raj et al., 2012, Cell Cycle 11(20): 3731--3738Rbf1 degron dysfunction enhances cellular DNA replication. [FBrf0219758] Raj et al., 2012, J. Biol. Chem. 287(50): 41835--41843Ubiquitination of Retinoblastoma Family Protein 1 Potentiates Gene-specific Repression Function. [FBrf0220180] Zhai et al., 2012, PLoS Genet. 8(3): e1002582Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Axelsson et al., 2011, BMC Bioinformatics 12: 342Extracting quantitative genetic interaction phenotypes from matrix combinatorial RNAi. [FBrf0215222] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Nicolay et al., 2011, Genes Dev. 25(4): 323--335Cooperation between dE2F1 and Yki/Sd defines a distinct transcriptional program necessary to bypass cell cycle exit. [FBrf0214348] Popova et al., 2011, J. Cell Sci. 124(24): 4203--4212Rb deficiency during Drosophila eye development deregulates EMC, causing defects in the development of photoreceptors and cone cells. [FBrf0217175] Sukhanova et al., 2011, Dev. Biol. 359(2): 190--198RBF and Rno promote photoreceptor differentiation onset through modulating EGFR signaling in the Drosophila developing eye. [FBrf0216493] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393]
Recent reviews (0)
All reviews listed in FlyBase were published before 2011