FlyBase Gene Report: Dmel\eIF3i

CYTOPLASMIC TRANSLATION INITIATION FACTORS -: Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).

Protein Function (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.

(UniProt, O02195)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\eIF3i or the JBrowse view of Dmel\eIF3i for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.44

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eIF3i-RA

FBtr0079053

NM_078754

1177

326

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

eIF3i-PA

FBpp0078689

36.2

326

4.97

NP_523478

AAF52183

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, O02195)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

O02195

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3i using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Gene Ontology (3 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

translation initiation factor activity

inferred from electronic annotation with InterPro:IPR027525

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

eukaryotic translation initiation factor 3 complex

inferred from electronic annotation with InterPro:IPR027525

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

inferred from sequence or structural similarity

(Lasko, 2000)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

organism | ubiquitous

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage | female

ovary

(Pisa et al., 2009)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3i in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eIF3i

View exonic expression by tissue for Dmel\eIF3i

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015834

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015834

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8882-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8882

Flygut - An atlas of the Drosophila adult midgut

FBgn0015834

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eIF3i

Transgenic constructs containing regulatory region of eIF3i

Deletions and Duplications ( 4 )

Disrupted in

Df(2L)BSC51

(Marygold et al., 2007)

Df(2L)ED7853

(Marygold et al., 2007)

Df(2L)Exel7021

(Marygold et al., 2007, Ryder, 2004.4.26)

Df(2L)Exel8012

(Marygold et al., 2007, Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^bbg-C96

eIF3i^HMS01387

lethal, with Scer\GAL4^pnr-MD237

eIF3i^GD14057

lethal, with Scer\GAL4^tin.CΔ4

eIF3i^GD14057

lethal | pupal stage, with Scer\GAL4^pnr-MD237

eIF3i^GD14057

lethal - all die during larval stage, with Scer\GAL4^Mef2.PR

eIF3i^GD14057

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eIF3i^GD14057

Phenotype manifest in

Allele

sarcomere, with Scer\GAL4^Mef2.PR

eIF3i^GD14057

Z disc, with Scer\GAL4^Mef2.PR

eIF3i^GD14057

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EIF3I

14 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eif3i

13 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Eif3i

7 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\eif3i

12 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\eif3i

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\eif-3.I

14 of 15

Yes

Arabidopsis thaliana (thale-cress) (2)

Atha\TRIP-1

9 of 9

Yes

Atha\AT2G46290

7 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\TIF34

14 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\sum1

12 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190AWK )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\eIF3i

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21387

Drosophila persimilis

Dper\GL26537

Drosophila willistoni

Dwil\GK24461

Drosophila virilis

Dvir\GJ17275

Drosophila mojavensis

Dmoj\GI15375

Drosophila grimshawi

Dgri\GH10244

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506NN )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018810

Glossina morsitans

Tsetse fly

Gmor\GMOY008116

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_14293

Mayetiola destructor

Hessian fly

Mdes\Mdes008748

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL015547

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013144

Anopheles darlingi

American malaria mosquito

Adar\ADAC008094

Anopheles gambiae

Malaria mosquito

Agam\EIF3I_ANOGA

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018275

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ018457

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06WF )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA007889

Danaus plexippus

Monarch butterfly

Dple\DPOGS213128-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL006522

Apis florea

Little honeybee

Aflo\2450640356

Apis mellifera

Western honey bee

Amel\Trip1

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16251

Bombus terrestris

Buff-tailed bumblebee

Bter\15209705

Linepithema humile

Argentine ant

Lhum\LH22202

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00280f

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG014962

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01850

Tribolium castaneum

Red flour beetle

Tcas\TC002391

Pediculus humanus

Human body louse

Phum\PHUM561140

Rhodnius prolixus

Kissing bug

Rpro\RPRC010290

Cimex lectularius

Bed bug

Clec\CLEC005430

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003750

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000798

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI083544

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_18510

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X071H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR001365

Ixodes scapularis

Black-legged tick

Isca\ISCW024524

Ixodes scapularis

Black-legged tick

Isca\ISCW000011

Stegodyphus mimosarum

African social velvet spider

Smim\KFM68458.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur10g02520

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_304176

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AIZ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Eif3i

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_008310gn

Ciona intestinalis

Vase tunicate

Cint\LOC100177148

Gallus gallus

Domestic chicken

Ggal\EIF3I

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

1 of 10

wmd

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF3I; eukaryotic translation initiation factor 3 subunit I

14 of 15

603911

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eIF3i - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - RagC-D

anti tag coimmunoprecipitation, anti tag western blot

(Tsokanos et al., 2016)

eIF3i - Ras85D

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

eIF3i - Rheb

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - eIF3b

anti bait coimmunoprecipitation, western blot

(Rode et al., 2018)

eIF3i - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - orb2

anti tag coimmunoprecipitation, Identification by mass spectrometry, western blot

(White-Grindley et al., 2014)

eIF3i - pix

tandem affinity purification, western blot

(Andersen and Leevers, 2007)

eIF3i - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3i - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix.

(UniProt, O02195 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59895

DroID - A comprehensive database of gene and protein interactions.

FBgn0015834

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33710

MIST (protein-protein) - An integrated Molecular Interaction Database

Trip1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-14

Cytogenetic map

25B5-25B5

Sequence location

2L:4,966,367..4,967,775 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

25B5-25B5

Limits computationally determined from genome sequence between P{lacW}l(2)k10004^k10004&P{EP}CG3036^EP963 and P{PZ}l(2)05714⁰⁵⁷¹⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

2-14

(FlyBase, 2016)

2-16.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (5)

Bloomington

14294

y¹; P{SUPor-P}eIF3i^KG06360/CyO; ry⁵⁰⁶

34978

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01387}attP2

Kyoto

114383

y^d2 w¹¹¹⁸ P{ey-FLP.N}2; P{SUPor-P}eIF3i^KG06360 P{neoFRT}40A/CyO y⁺

VDRC

v103141

P{KK107698}VIE-260B

v28976

w¹¹¹⁸; P{GD14057}v28976

Genomic Clones (12)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (297)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD24026

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0015834

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0015834

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33710

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Trip1 CG8882

(Kodira, 1999.11)

Source for identity of: eIF3i Trip1

(Marygold et al., 2017)

Source for database merge of

Additional comments

Other Comments

Cloned by PCR as being similar to Hsap\TRIP-1.

(Cho et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 50 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33710

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O02195

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0015834

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0015834

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8882

Flygut - An atlas of the Drosophila adult midgut

FBgn0015834

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33710

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002391

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8882

modMine - A data warehouse for the modENCODE project

FBgn0015834

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O02195

Linkouts

BioGRID - A database of protein and genetic interactions.

59895

DroID - A comprehensive database of gene and protein interactions.

FBgn0015834

DRSC - Results frm RNAi screens

FBgn0015834

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0015834

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8882-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0015834

FlyMine - An integrated database for Drosophila genomics

FBgn0015834

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33710

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

MIST (protein-protein) - An integrated Molecular Interaction Database

Trip1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG8882

(Kolkhof et al., 2017, Marygold et al., 2017, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Fukuoh et al., 2014, White-Grindley et al., 2014, Yoshigi et al., 2013, Cammarato et al., 2011, Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Pisa et al., 2009, Hughes et al., 2008, Lasko, 2000)

DmTRIP

(Cho, 1997.2.26)

TRIP1

(Ellis et al., 2015)

Trip1

(Dent et al., 2015, Wójtowicz et al., 2015, Wang et al., 2014, White-Grindley et al., 2014, Kwon et al., 2013, Yoshigi et al., 2013, Friedman et al., 2011, Dekanty et al., 2010, Mourikis et al., 2010, Pisa et al., 2009, Fisher et al., 2008, Somma et al., 2008, Marygold et al., 2007, Stuart et al., 2007, Stanyon et al., 2004)

eIF-3i

(Grobler et al., 2018)

eIF-3β

(Pisa et al., 2009)

eIF3-S2

(Kurshakova et al., 2019, Tsokanos et al., 2016, Olesnicky et al., 2014, Somma et al., 2008, Andersen and Leevers, 2007, Lasko, 2000)

eIF3i

(Marygold et al., 2017, Majzoub et al., 2014, Yao and Fox, 2011)

Name Synonyms

Trip1

(Cammarato et al., 2011, Somma et al., 2008, Cho et al., 1996)

eukaryotic translation initiation factor 3 subunit i

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (69)

Report Sections

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