iro, iroquois, Iro-C
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.45
There is only one protein coding transcript and one polypeptide associated with this gene
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\caup using the Feature Mapper tool.
When tin expression becomes restricted to dorsal and ventral clusters that represent cardiac and visceral mesodermal cells at stage 11, there is a stronger expression of caup mRNA around the ventral clusters of tin-positive cells.
Mirzoyan and Pandur (FBrf0222881) use an antibody which detects both ara and caup proteins, which have similar expression patterns (see transcript expression for ara and caup). During stage 13, single ara/caup-expressing cells form alongside the row of Mef2-positive myocardial cells, and are between the eve-positive pericardial cells by stage 15.
Using an anti-caup antibody that recognizes both ara and caup, expression is detected in groups of cells in the presumptive visceral trunk mesoderm at early embryonic stage 11. By mid stage 11, they are expressed at the same dorsoventral level in the visceral mesoderm and the in the dorso-lateral ectoderm. Expression declines in the visceral mesoderm at late stage 11 and then is detected in the somatic mesoderm in promuscular clusters from which a subset of muscle progenitors expressing ara and caup will segregate in stage 12. Expression is maintained in sibling muscle founder myoblasts derived from araFBtr0091350:pb-XRcaup-expressing progenitors and in the muscles they give rise to, namely LT1-4, dorsal transverse 1, and segment border muscle. ara and caup muscle expression was compared that of several muscle identity genes.
GBrowse - Visual display of RNA-Seq signalsView Dmel\caup in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: caup CG10605
S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.
The localised expression of the three genes of the iroquois complex - IRO-C (ara, caup and mirr) specifies the identity of dorsal cells in the eye. Juxtaposition of IRO-C-expressing and non-expressing cells forms a straight border that promotes growth and serves as a pattern-organising centre in the eye disc.
The genes of the Iroquois complex (ara, caup and mirr) are necessary for notum specification. The Iro-C genes establish a signalling system that appears to organize development in the notum and dorsal wing hinge (tegula and sclerite) territories.
When the Iroquois complex is absent, notum cells are transformed into hinge (tegula and sclerite) cells.
A member of the IRO-C (Iroquois complex).
The genes of the IRO-C are coordinately regulated and implicated in similar developmental processes. They are regulated in the eye by the Pc-group and trx-group genes, but not by classical modifiers of position effect variegation. Ventral silencing of the whole IRO-C in the eye occurs at the level of chromatin structure in a manner similar to that of the homeotic gene complexes, perhaps by local compaction of the region into a heterochromatin-like structure involving Pc-group products.
ara-caup expression at patches on the wing, located one at each side of the DV compartment border, is mediated by the hh signal through its induction of high levels of ci protein in anterior cells near to the AP compartment border. High levels of ci activate dpp expression and together ci and dpp positively control ara-caup expression. The posterior border of the patches is defined by repression by en. wg accumulation at the DV border sets, also by repression, the gap between the two patches.
Gene titration method has been used to search for genes involved in the determination of sense organs. A mutation of ara and caup (formerly iro) demonstrates an interaction with Df(4)M101-62f, a chromosome known to alter the development of the PNS.
In mutants for the Iroquois gene complex, two lateral bands of the notum are completely devoid of sense organs.
The "caup" gene is named after a hero of the Araucanian American-Indian tribe.
The 'Iroquois' gene complex is named after the native American Iroquois tribe, also called Mohawks, which shaved all but a medial stripe of hairs on the head.
"Iroquois" is the name given to the gene complex by analogy with the American tribe that have a haircut ascribed to the Mohawk nation.