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Gene Dmel\wun

General Information
Symbol	Dmel\wun	Species	D. melanogaster
Name	wunen	Annotation symbol	CG8804
Feature type	protein_coding_gene	FlyBase ID	FBgn0016078
Gene Model Status	Current	Stock availability	26 publicly available
Also Known As	l(2)k16806
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	45D3-45D4	Sequence location	2R:5,288,036..5,300,968 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene wunen is referred to in FlyBase by the symbol Dmel\wun (CG8804, FBgn0016078). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: phosphatase activity. There is experimental evidence that it is involved in the biological process: germ cell migration; germ cell repulsion; open tracheal system development; septate junction assembly. 47 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/larval lateral trunk; bouton; embryonic proventriculus; embryonic/larval dorsal trunk; germline cell; intersegmental nerve; presumptive embryonic/larval digestive system; synapse; embryonic/larval transverse connective. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Phosphatidic acid phosphatase type 2/haloperoxidase; Phosphatidic acid phosphatase/chloroperoxidase, N-terminal. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 12-18 embryonic stages, during late larval stages, during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1631573_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of wun. Gene sequence location is 2R:5288036..5300968.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 45D3-45D4   Limits computationally determined from genome sequence between P{lacW}Pknk11209 and P{lacW}wunk09507&P{lacW}l(2)k12402k12402
Experimentally Determined Cytological Location
Cytogenetic map Notes References 45D4-45D5   (determined by in situ hybridisation)   (Spradling et al., 1999) 45D4-45D5   (determined by in situ hybridisation)   (Zhang et al., 1996) 45D4-45D5     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Zhang et al., 1996)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\wun for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters. Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.48
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) wun-RA FBtr0088553  NM_057845   1626   300 wun-RB FBtr0088552  NM_165674   2026   379 wun-RC FBtr0306111  NM_001259294   1563   364
Additional Transcript Data & Comments
Reported size (kB)	1.622 (longest cDNA) (Zhang et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein wun-PA   FBpp0087636   33.9   300   9.11     NP_477193   AAM71066 wun-PB   FBpp0087635   42.8   379   8.45     NP_724787   AAF58942 wun-PC   FBpp0297248   41.3   364   8.95     NP_001246223   AFH07978
Additional Polypeptide Data & Comments
Reported size (kDa)	300 (aa) (Zhang et al., 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Phosphatidic acid phosphatase type 2/haloperoxidase (IPR000326) Phosphatidic acid phosphatase/chloroperoxidase, N-terminal (IPR016118)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA940783   AE013599 AAF58942   AE013599 AAM71066   AC008341   AF145595 AAD38570   AE013599 AFH07978   AI062852   AI401851   AI514677   AI518107   AQ025894   AQ026117   AQ073398   AQ073721   AY046533 AAL34392   BI164505   BI216140   BI241795   BI370211   BI374044   BI486317   BI486929   BI580438   BP557646   BT001729 AAN71484   BT099641   CK659648   CK659964   CO191706   CO191753   CO276346   CO320936   CO335632   CW848181   CZ471901   CZ473592   CZ473687   EC051723   EC052206   EC054646   EC054650   EC055944   EC066749   EC204786   EC208943   EC265914   GH748380   GH749593   GH750017   GH750066   GH750191   GH751838   GH751915   GH752218   GH753350   GH753420   GH754546   GH755158   GH755546   GH755569   GH756734   GH757588   GH758162   GH759327   GH760283   GH761141   GH770890   GH771435   GH771919   GH773261   GH774248   GH774762   GH776173   GH780034   GH781232   GH782070   GH783619   GH783849   GH786156   GH786613   GH788330   GH788496   GH790111   GH792526   GH792836   GH793138   GH795408   GH795977   GH796351   GH797064   GH798768   GH798874   GH799981   GH800150   GH800899   GH801679   GH801958   GH802523   GH803567   GH805691   GH806512   GH807694   GH808680   GH809587   GH811425   GH811946   GH815968   GH817573   GH817643   GH818165   GH819276   GH820722   GH821140   GH822157   GH823818   GH824132   GH824522   GH825217   GH826006   GH828459   GH832292   GH833203   GH835464   GH835625   GH836672   GH838788   GH842496   GH843863   GH844151   GH844648   GH845019   GH846933   GH851387   GH853353   GH854901   GH858046   GH858856   GH859772   GH860567   GH860648   GH860695   GH861362   GH861585   GH861684   GH861710   GH863232   GH864420   GH865220   GH865391   GH865620   GH865956   GH866680   GH873575   GH875129   GH875179   GH877131   GH877897   GH878152   GH878346   GH880725   GH884947   GH885307   GH886047   GH886363   GH887600   GH888346   GH889977   GH890515   GH891539   GH892457   GH893763   GH895042   GH895903   GH896672   GH901976   GH902101   GH904270   GH904609   GH907333   GH907814   GH910472   GH910897   GH911325   GH911375   GH912024   GH912158   GH913695   GH914135   GH916279   GH917106   GH917701   GH918489   GH918542   GH921985   GH923232   GH926751   GH927090   GH930598   GH930966   GH933233   GH935512   GH938371   U73822 AAC47449   Z71084
UniProtKB/Swiss-Prot	Q9V576
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage   posterior midgut primordium | dorsal   (Zhang et al., 1997) ectoderm | restricted   (Zhang et al., 1997) inclusive hindgut primordium   (Zhang et al., 1997) embryonic stage | late   presumptive embryonic/larval central nervous system   (Zhang et al., 1997) embryonic stage 10   posterior midgut primordium | restricted   (Starz-Gaiano et al., 2001) Comment:excludes part where germ cells exit the gut hindgut proper primordium   (Starz-Gaiano et al., 2001) anterior midgut primordium   (Starz-Gaiano et al., 2001) embryonic stage 10 -- 12   neuroblast | lateral & subset   (Starz-Gaiano et al., 2001) embryonic stage 10 -- 13   epidermis | segmentally repeated   (Starz-Gaiano et al., 2001) embryonic stage 13 -- 16   embryonic foregut   (Fisher et al., 2012) embryonic/larval visceral muscle   (Fisher et al., 2012)
Additional Descriptive Data
	wun transcripts are first expressed at high levels in the hindgut primordium, and are expressed in a segmentally repeated pattern in the ectoderm. They are later expressed on the dorsal side of the posterior midgut. In germ band extended embryos, wun is expressed in the ectoderm in a medial band throughout the trunk. In germ band retracted embryos, wun is expressed in the gut, nervous system, and ectoderm. (Zhang et al., 1997)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
apical part of cell inferred from direct assay (Ile et al., 2012) septate junction inferred from direct assay (Ile et al., 2012)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult crop, adult hindgut, adult salivary gland. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop    781.6 Adult Midgut    266.5 Adult Hindgut    515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland    804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop  (781.6) Adult Midgut    266.5 Adult Hindgut  515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland  (804.1) Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop    781.6 Adult Midgut    266.5 Adult Hindgut    515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland    804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop    781.6 Adult Midgut    266.5 Adult Hindgut    515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland    804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop    781.6 Adult Midgut    266.5 Adult Hindgut    515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland    804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop  781.6 Adult Midgut    266.5 Adult Hindgut  515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland  804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop    781.6 Adult Midgut    266.5 Adult Hindgut    515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland    804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    74.9 Larval Midgut    259.7 Larval Hindgut    207.6 Larval Malpighian Tubules    198 Larval Fat Body    14.6 Larval Salivary Gland    73.3 Larval Trachea    70 Larval Carcass    144.9 Adult Head    137.8 Adult Eye    108.65 Adult Brain    217.1 Adult Thoracic-Abdominal Ganglion    268.6 Adult Crop    781.6 Adult Midgut    266.5 Adult Hindgut    515.7 Adult Malpighian Tubules    203.4 Adult Fat Body    68.8 Adult Salivary Gland    804.1 Adult Heart    86.975 Adult VirginFemale Spermatheca    64.9 Adult InseminatedFemale Spermatheca    68.9 Adult Ovary    86 Adult Testis    27.5 Adult Male Accessory Gland    84.3 Adult Carcass    89.5 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    137.8   NA  Eye    108.65   NA  Brain    217.1   74.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    268.6   NA  Crop    781.6   259.7  Midgut    266.5   207.6  Hindgut    515.7   198  Malpighian Tubules    203.4   no informative data  Fat Body    68.8   73.3  Salivary Gland    804.1   NA  Heart    86.975   70  Trachea    NA   NA  VirginFemale Spermatheca    64.9   NA  InseminatedFemale Spermatheca    68.9   NA  Ovary    86   NA  Testis    27.5   NA  Male Accessory Gland    84.3   144.9  Carcass    89.5 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    100 digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering  (100) digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    100 digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    100 digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    100 digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering  (100) digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    100 digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    10 central nervous system, larvae L3    9 central nervous system, pupae P8    11 head, virgin 1-day female    21 head, virgin 4-day female    14 head, virgin 20-day female    9 head, mated 1-day female    19 head, mated 4-day female    13 head, mated 20-day female    8 head, mated 1-day male    25 head, mated 4-day male    20 head, mated 20-day male    22 salivary gland, larvae L3 wandering    9 salivary gland, white prepupae    24 digestive system, larvae L3 wandering    100 digestive system, 1-day adult    20 digestive system, 4-day adult    25 digestive system, 20-day adult    21 fat body, larvae L3 wandering    2 fat body, white prepupae    12 fat body, pupae P8    5 carcass, larvae L3 wandering    21 carcass, 1-day adult    9 carcass, 4-day adult    11 carcass, 20-day adult    8 ovary, virgin 4-day female    19 ovary, mated 4-day female    21 testis, mated 4-day male    4 accessory gland, mated 4-day male    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 12-18 embryonic stages, during late larval stages, during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr    36 embryo 12-14hr    39 embryo 14-16hr    50 embryo 16-18hr    61 embryo 18-20hr    42 embryo 20-22hr    38 embryo 22-24hr    43 larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    31 larva L3 puffstage 7-9    59 white prepupae new    76 white prepupae 12hr    42 white prepupae 24hr    26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP    46 adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr  (36) embryo 12-14hr  (39) embryo 14-16hr  (50) embryo 16-18hr  (61) embryo 18-20hr  (42) embryo 20-22hr  (38) embryo 22-24hr  (43) larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6  (31) larva L3 puffstage 7-9  (59) white prepupae new  (76) white prepupae 12hr  (42) white prepupae 24hr  26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP  (46) adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr    36 embryo 12-14hr    39 embryo 14-16hr    50 embryo 16-18hr    61 embryo 18-20hr    42 embryo 20-22hr    38 embryo 22-24hr    43 larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    31 larva L3 puffstage 7-9    59 white prepupae new    76 white prepupae 12hr    42 white prepupae 24hr    26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP    46 adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr    36 embryo 12-14hr    39 embryo 14-16hr    50 embryo 16-18hr    61 embryo 18-20hr    42 embryo 20-22hr    38 embryo 22-24hr    43 larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    31 larva L3 puffstage 7-9    59 white prepupae new    76 white prepupae 12hr    42 white prepupae 24hr    26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP    46 adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr    36 embryo 12-14hr    39 embryo 14-16hr    50 embryo 16-18hr    61 embryo 18-20hr    42 embryo 20-22hr    38 embryo 22-24hr    43 larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    31 larva L3 puffstage 7-9    59 white prepupae new    76 white prepupae 12hr    42 white prepupae 24hr    26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP    46 adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr  (36) embryo 12-14hr  (39) embryo 14-16hr  (50) embryo 16-18hr  (61) embryo 18-20hr  (42) embryo 20-22hr  (38) embryo 22-24hr  (43) larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6  31 larva L3 puffstage 7-9  (59) white prepupae new  (76) white prepupae 12hr  (42) white prepupae 24hr  26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP  (46) adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr    36 embryo 12-14hr    39 embryo 14-16hr    50 embryo 16-18hr    61 embryo 18-20hr    42 embryo 20-22hr    38 embryo 22-24hr    43 larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    31 larva L3 puffstage 7-9    59 white prepupae new    76 white prepupae 12hr    42 white prepupae 24hr    26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP    46 adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    19 embryo 02-04hr    17 embryo 04-06hr    16 embryo 06-08hr    17 embryo 08-10hr    23 embryo 10-12hr    36 embryo 12-14hr    39 embryo 14-16hr    50 embryo 16-18hr    61 embryo 18-20hr    42 embryo 20-22hr    38 embryo 22-24hr    43 larva L1    25 larva L2    20 larva L3 12hr old    13 larva L3 puffstage 1-2    18 larva L3 puffstage 3-6    31 larva L3 puffstage 7-9    59 white prepupae new    76 white prepupae 12hr    42 white prepupae 24hr    26 pupae 2d postWPP    19 pupae 3d postWPP    16 pupae 4d postWPP    46 adult male 01day    20 adult male 05day    19 adult male 30day    22 adult female 01day    25 adult female 05day    23 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    8 Schneider line 2 Sg4    7 embryonic 1182-4H    8 embryonic GM2    12 embryonic Kc167    9 embryonic S1    5 embryonic S3    3 leg disc CME L1    8 wing disc CME-W2    6 wing disc ML-DmD8    5 wing disc ML-DmD9    7 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    7 wing disc ML-DmD32    8 haltere disc ML-DmD17-c3    9 eye-antennal disc ML-DmD11    6 antennal disc ML-DmD20-c5    7 mixed discs ML-DmD4-c1    6 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    4 ovary fGS/OSS    5 ovary OSC    1 ovary OSS    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    34 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3  (27) Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult  (27) Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (34) Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3  (27) Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (27) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (29) Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    34 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    34 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    34 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3  27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult  27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult  (34) Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult  26 Ethanol 2.5% 3 hrs, larvae L3  27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    34 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    15 heat shock, 4-day adult    16 Cadmium 50 mM 6 hrs, larvae L3    11 Cadmium 50 mM 12 hrs, larvae L3    27 Cadmium 50 mM 48 hrs, 4-day adult    19 Cadmium 100 mM 48 hrs, 4-day adult    27 Copper 0.5 mM 12 hrs, larvae L3    8 Copper 15 mM 48 hrs, 4-day adult    34 Zinc 5 mM 12 hrs, larvae L3    16 Zinc 4.5 mM 48 hrs, 4-day adult    26 Ethanol 2.5% 3 hrs, larvae L3    27 Ethanol 5% 3 hrs, larvae L3    20 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    27 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    29 Paraquat 10 mM 48 hrs, 4-day adult    24 Rotenone 2 μg 12 hrs, larvae L3    13 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_25 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • GH02203 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016078 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8804-RB FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for wun Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | recessive wunEMS4 wunEMS23 wunEMS41 wunk09507 wunk10201 wunk15909 wunk16806 partially lethal - majority die wunEMS9 viable wunEY11935 viable, with Scer\GAL4[-]: wunEP2607 viable, with Scer\GAL4pnr-MD237 wunGD1640 Sterility Allele female sterile wunCE wunCN wunEMS9 fertile wunEY11935 Other Phenotypes Allele learning defective | adult stage wun12.112 neuroanatomy defective wunk09507 neuroanatomy defective | larval stage, with Scer\GAL4elav-C155 wunEP2208 wild-type wun+tNN Phenotype manifest in Allele anterior fascicle & synapse, with Scer\GAL4elav-C155 wunEP2208 anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155 wunEP2208 bouton wunk09507 embryonic/larval dorsal trunk wununspecified embryonic/larval lateral trunk wununspecified embryonic/larval transverse connective wununspecified embryonic proventriculus (with Df(2R)wun-GL) wunk15909 germline cell wunCE wunCN germline cell, with Scer\GAL4[-]: wunEP2607 germline cell, with Scer\GAL4da.G32 wunScer\UAS.T:Avic\GFP-EGFP germline cell, with Scer\GAL4how-24B, Scer\GAL4twi.PG wunScer\UAS.cZa germline cell, with Scer\GAL4twi.PG wunScer\UAS.T:Avic\GFP-EGFP germline cell | embryonic stage | male wunCE germline cell | female wunk10201 germline cell | female, with Scer\GAL4twi.PG wunScer\UAS.cZa germline cell | male wunk10201 germline cell | male, with Scer\GAL4twi.PG wunScer\UAS.cZa intersegmental nerve, with Scer\GAL4elav-C155 wunEP2208 presumptive embryonic/larval digestive system (with Df(2R)wun-GL) wunk15909 synapse wunk09507
Classical Alleles ( 37 )
For All Classical Alleles Show
Allele of wun Class Mutagen Stocks Known lesion wunk10201 loss of function allele P-element activity 2 Yes wunk16806 P-element activity 2 Yes wun5-HA-1200 1 -- wun5-HA-2881 1 -- wunc01445 1 -- wune02143 1 -- wunEMS23 ethyl methanesulfonate 1 -- wunEMS41 ethyl methanesulfonate 1 -- wunEMS4 ethyl methanesulfonate 1 -- wunEY11935 P-element activity 1 -- wunk09507 P-element activity 1 Yes wunk15909 P-element activity 1 -- wunMI06541 1 -- wunNP0855 1 -- wunNP1243 1 -- wunNP2127 1 -- wunNP3279 1 -- wunNP5293 1 -- wun12.112 0 Yes wun49 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes wun5-HA-2269 0 -- wun5-HA-2275 0 -- wunCE loss of function allele 0 -- wunCN 0 Yes wund00931 P-element activity 0 -- wund03854 P-element activity 0 -- wund04014 P-element activity 0 -- wunEMS9 ethyl methanesulfonate 0 -- wunEP2208 P-element activity 0 Yes wunEP2525 0 -- wunEP2607 P-element activity 0 Yes wunEP2650ex34 Delta2-3 0 Yes wunEP652 P-element activity 0 -- wunGS10009 0 Yes wunk12382 0 -- wunPBacF48 0 -- wununspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of wun Class Mutagen Stocks Known lesion wunGD15706 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes wunGD1640 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes wunHMS00424 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes wunHMS00425 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes wun+tNN in vitro construct - genomic fragment 0 Yes wunD248T.Scer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes wunNIG.8804R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes wunNX in vitro construct - genomic fragment 0 Yes wunScer\UAS.cZa in vitro construct - regulatory fusion 0 Yes wunScer\UAS.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)11 (Kozopas, 2003.3, Georgiev et al., 2012) Df(2R)Np5 (Spradling et al., 1999, Kozopas, 2003.3, BDGP Project Members, 1994-1999, Zhang et al., 1996, Georgiev et al., 2012) Df(2R)NYX-D15 (Sano et al., 2005) Df(2R)w45-19g (Georgiev et al., 2012) Df(2R)w45-30n (BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)w73-1 (Zhang et al., 1996, Georgiev et al., 2012) Df(2R)wun-GL (Matthews, 1996.2.2, Zhang et al., 1996, Georgiev et al., 2012, Sano et al., 2005) In(2LR)TE35B-15LL2R (Zhang et al., 1996) In(2R)G63Lw45-73nR (Zhang et al., 1996)
Not disrupted in	Df(2R)H3C1 (Zhang et al., 1996) Df(2R)H3D3 (Zhang et al., 1996) Df(2R)H3E1 (Zhang et al., 1996) Df(2R)NCX10 (Zhang et al., 1996) Df(2R)NCX11 (Zhang et al., 1996) Df(2R)Np1 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)Np3 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)Np4 (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)P14TE (Zhang et al., 1996) Df(2R)w45-30n (BDGP Project Members, 1994-1999) Df(2R)w73-1 (BDGP Project Members, 1994-1999, Zhang et al., 1996) Df(2R)wun-GL (Zhang et al., 1996) Df(2R)XE-916 (BDGP Project Members, 1994-1999) In(2LR)TE35B-15 (Zhang et al., 1996) In(2R)G63Lw45-73nR (Spradling et al., 1999, BDGP Project Members, 1994-1999, Zhang et al., 1996)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1640} NA P{GD15706} NA P{NIG.8804R} NA P{TRiP.HMS00424} NA P{TRiP.HMS00425} NA P{UAS-wun.D248T.GFP} NA P{UAS-wun.EGFP} NA P{UAS-wun.Z} NA characterization construct P{NN} NA P{NX} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(2)SH0273SH0273 No P{lacW}wunk09507 No P{lacW}wunk10201 No P{lacW}wunk15909 No P{lacW}wunk16806 No P{PZ}l(2)0349703497 No insertion of mobile activating element P{EP}wunEP652 NA P{EP}wunEP2208 NA P{EP}wunEP2525 NA P{EP}wunEP2607 NA P{EPgy2}wunEY11935 NA P{GSV6}wunGS10009 NA P{XP}wund00931 NA P{XP}wund03854 NA P{XP}wund04014 NA miscellaneous insertions Mi{MIC}wunMI06541 NA P{RS5}wun5-HA-1200 NA P{RS5}wun5-HA-2269 NA P{RS5}wun5-HA-2275 NA P{RS5}wun5-HA-2881 NA PBac{3xP3-EYFP,p-tTA-K10}wunPBacF48 NA PBac{PB}wunc01445 NA PBac{RB}wune02143 NA insertion of enhancer trap binary system P{GawB}wun12.112 No P{GawB}wunNP0855 No P{GawB}wunNP1243 No P{GawB}wunNP2127 No P{GawB}wunNP3279 No P{GawB}wunNP5293 No
Gene Ontology: Function, Process & Cellular Component ( 16 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
phosphatase activity inferred from direct assay (Burnett and Howard, 2003)
Biological Process
CV term Evidence References
germ cell migration inferred from mutant phenotype (Sano et al., 2005) germ cell repulsion inferred from genetic interaction with wun2 (Renault et al., 2010) inferred from mutant phenotype (Sano et al., 2005) open tracheal system development inferred from mutant phenotype (Ile et al., 2012) septate junction assembly inferred from mutant phenotype (Ile et al., 2012)
Cellular Component
CV term Evidence References
apical part of cell inferred from direct assay (Ile et al., 2012) septate junction inferred from direct assay (Ile et al., 2012)
Terms Based on Predictions or Assertions ( 11 terms )
Molecular Function
CV term Evidence References
phosphatidate phosphatase activity inferred from sequence or structural similarity (Hayden and Howard, 1999) non-traceable author statement (Morrison et al., 2000) non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2002.6.15) protein homodimerization activity traceable author statement (Pyne et al., 2004)
Biological Process
CV term Evidence References
dephosphorylation non-traceable author statement (Morrison et al., 2000) non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2002.6.15) embryonic pattern specification traceable author statement (Pyne et al., 2004) germ cell migration non-traceable author statement (Russell, 2001.7.18) non-traceable author statement (assigned by UniProtKB) (Zhang et al., 1997) traceable author statement (Wylie, 1999, Mueller, 2002) germ cell programmed cell death traceable author statement (Coffman, 2003) germ cell repulsion traceable author statement (Coffman, 2003, Ribeiro et al., 2003, Luquain et al., 2003, Raz, 2004) pole cell migration traceable author statement (Howard, 1998)
Cellular Component
CV term Evidence References
integral to membrane non-traceable author statement (assigned by UniProtKB) (Swiss-Prot Project Members, 2002.6.15) membrane inferred from electronic annotation with InterPro:IPR000326 (FlyBase Curators et al., 2004-) plasma membrane inferred from sequence or structural similarity (assigned by UniProtKB) (Zhang et al., 1997)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
wun - B52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG10418 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG11266 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG17768 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG3224 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG3862 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG4553 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG4612 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG5746 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG7878 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Cct5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Cdc5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - DnaJ-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - SC35 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Socs44A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - mRpL39 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - pUf68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - sls anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wun - snf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions wun allele Gene References wunCE wun2 (Hanyu-Nakamura et al., 2004) unspecified L (Singh et al., 2005) pbl (Gregory et al., 2007) rdgA (Garcia-Murillas et al., 2006)
External Data
Linkouts	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0016078 DroID - A comprehensive database of gene and protein interactions. • FBgn0016078 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35966
Orthologs
OrthoDB Orthologs (57) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6PVPJW Dipteran inclusive ortholog search EOG65497J Insect inclusive ortholog search EOG6KPS6Z Arthropod inclusive ortholog search EOG6WWQ1H Metazoa inclusive ortholog search EOG6TBH5Q
Orthologs in Drosophila Species (EOG6PVPJW)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\wun     Drosophila simulans   Dsim\GD10076 Y   Drosophila sechellia   Dsec\GM20603 Y   Drosophila erecta   Dere\GG10557 Y   Drosophila yakuba   Dyak\GE22302 Y   Drosophila ananassae   Dana\GF13080 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21332 Y   Drosophila persimilis   Dper\GL16886 Y   Drosophila willistoni   Dwil\GK21745 Y   Drosophila virilis   Dvir\GJ18026 Y   Drosophila mojavensis   Dmoj\GI19743 Y   Drosophila grimshawi   Dgri\GH21309 Y
Orthologs in non-Drosophila Dipterans (EOG65497J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007322   Anopheles gambiae Malaria mosquito  Agam\AGAP012445   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ000134
Orthologs in non-Dipteran Insects (EOG6KPS6Z)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49207 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG013741 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH23602 Y Atta cephalotes Leafcutter ant  Acep\ACEP14034 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO21272 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18423 Y Linepithema humile Argentine ant  Lhum\LH16928 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB19000 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI005610 Y Bombyx mori Silkmoth  Bmor\BGIBMGA003966 Y Pediculus humanus Human body louse  Phum\PHUM389600 Y Tribolium castaneum Red flour beetle  Tcas\TC015891 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_15830 Y
Orthologs in non-Insect Arthropods (EOG6WWQ1H)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_319296 Y Daphnia pulex Water flea  Dpux\JGI_V11_197078 Y Daphnia pulex Water flea  Dpux\JGI_V11_319297 Y Daphnia pulex Water flea  Dpux\JGI_V11_319295 Y Ixodes scapularis Deer tick  Isca\ISCW015634 Y
Orthologs in non-Arthropod Metazoa (EOG6TBH5Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\F13E6.5 Y Caenorhabditis elegans Nematode  Cele\F53C3.13 Y Caenorhabditis elegans Nematode  Cele\T28D9.3 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011127gn Y Danio rerio Zebrafish  Drer\ENSDARG00000053381 Y Danio rerio Zebrafish  Drer\ENSDARG00000002231 Y Danio rerio Zebrafish  Drer\ppap2b Y Danio rerio Zebrafish  Drer\zgc:158309 Y Danio rerio Zebrafish  Drer\CH211-206A19.2 Y Danio rerio Zebrafish  Drer\ENSDARG00000079790 Y Xenopus tropicalis Western clawed frog  Xtro\ppap2a Y Xenopus tropicalis Western clawed frog  Xtro\ppap2b Y Xenopus tropicalis Western clawed frog  Xtro\ppap2c Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000017268 Y Gallus gallus Domestic chicken  Ggal\PPAP2B Y Gallus gallus Domestic chicken  Ggal\PPAP2A Y Mus musculus House mouse  Mmus\ENSMUSG00000021759 Y Mus musculus House mouse  Mmus\Ppap2b Y Rattus norvegicus Norway rat  Rnor\Ppap2b Y Rattus norvegicus Norway rat  Rnor\Ppap2c Y Rattus norvegicus Norway rat  Rnor\Ppap2a Y Homo sapiens Human  Hsap\PPAP2C Y Homo sapiens Human  Hsap\PPAP2B Y Homo sapiens Human  Hsap\PPAP2A Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\PPAP2C     Hsap\PPAP2B     Hsap\PPAP2A
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD10076 Drosophila sechellia Dsec\GM20603 Drosophila erecta Dere\GG10557 Drosophila yakuba Dyak\GE22302 Drosophila ananassae Dana\GF13080 Drosophila pseudoobscura pseudoobscura Dpse\GA21332 Drosophila persimilis Dper\GL16886 Drosophila willistoni Dwil\GK21745 Drosophila virilis Dvir\GJ18026 Drosophila mojavensis Dmoj\GI19743 Drosophila grimshawi Dgri\GH21309
Stocks & Reagents
Stocks Listed in FlyBase ( 26 )
Bloomington	6951 wunEMS23/CyO; ry506 6950 wunEMS4/CyO, amosRoi-1 6952 wunEMS41/CyO, amosRoi-1
Harvard	- PBac{PB}wunc01445 - PBac{RB}wune02143
Kyoto	104437 w*; P{GawB}wunNP3279 125136 w1118; P{RS5}wun5-HA-1200 125726 w1118; P{RS5}wun5-HA-2881
VDRC	v6446 w1118; P{GD1640}v6446/TM3 v6447 w1118; P{GD1640}v6447/TM3 v51090 w1118; P{GD15706}v51090/TM3 v51091 w1118; P{GD15706}v51091/TM3
	More stocks available...
Genomic Clones ( 2 )
	BACR14J24 BACR15P11 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 187 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH02203 RE70417
Other clones	DPiM_GH02203 MIP13371
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD23023 LD37528 LD41163 LP17554 LP18135 RE04203 RE23069 RE38964 RE56652 RE61554 RE69734 RE74632
Other clones	165976 184100 542568 CK01.146D.C8 EC31701 EC31765 EC051723 EC052206 EC054646 EC054650 EC055944 EK120544 EK288845 EN10855 FGM310D11 RP003013435 RP003019139 RP003020967 RP003033487 RP003034948 RP003035426 RP003043171 RP003049196 RP003052056 RP003053314 RP003053790 RP003062812 RP003075102 RP003075463 RP003075590 RP003081966 RP003084220 RP003090980 RP003093408 RP003104533 RP003120919 RP003122551 RP003126420 RP003129873 RP003131111 RP003131466 RP003134313 RP003136103 RP003137672 RP003138071 RP003139085 RP003142437 RP003143872 RP003143982 RP003149222 RP003156512 RP003162262 RP003164526 RP003169256 RP003170878 RP003177034 RP003178377 RP003179885 RP003180105 RP003183099 RP003183613 RP003187041 RP003188584 RP003193387 RP003194855 RP003199658 RP003201373 RP003203802 RP003204685 RP003205530 RP003206817 RP003210441 RP003211681 RP003212557 RP003215137 RP003216848 RP003218775 RP003219676 RP003222255 RP003225955 RP003242888 RP003245275 RP003253986 RP003254068 RP003254855 RP003256748 RP003268574 RP003269124 RP003272196 RP003272979 RP003278443 RP003281557 RP003282549 RP003284132 RP003293378 RP003293545 RP003297015 RP003297778 RP003301394 RP003314036 RP003318193 RP003319952 RP003320355 RP003335043 RP003337116 RP003338644 RP003347983 RP003350117 RP003354595 RP003364089 RP003369825 RP003379042 RP003380295 RP003380598 RP003381473 RP003402504 RP003406245 RP003409828 RP003420053 RP003421553 RP003426054 RP003445933 RP003451738 RP003455291 RP003460923 RP003464439 RP003468459 RP003475745 RP003479512 RP003492979 RP003495917 RP003497946 RP003500082 RP003502932 RP003511891 RP003512922 RP003518803 RP003533198 RP003534816 RP003541620 RP003543657 RP003564547 RP003564745 RP003567559 RP003567623 RP003571499 RP003571936 RP003579646 RP003582277 RP003587716 RP003587796 RP003591787 RP003594479 RP003596859 RP003601669 RP003608193 RP003608969 RP003623934 RP003625737 RP003625761 RP003629759 RP003632293 RP003634306 RP003646673 RP003651778 RP003653396 RP003654958 RP003662452 RP003667549 RP003679596 RP003680168
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0016078 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35966
Antibody Information
Other Information
Discoverer
Etymology
	wun is named after a marshal in Chinese heaven, who was banished to earth in the form of a pig for causing a nuisance. Still causing a nuisance on earth, he was adopted by a monk named Tang after being outwitted in combat by the monk's monkey, Wukong. He was given the name 'Wunen', and taken on a trek to India to fetch Buddhist bark. Despite threats of further beatings, Wunen persisted in wandering off to steal food and chase beautiful girls. It is this wandering aspect of his behavior that gives the name 'wunen' to the wun gene. (Zhang and Howard, 1997.2.24)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: wun l(2)k16806 (Rabinow, 2004.12.29)
Additional comments	"l(2)03497" may correspond to "wun"; the P{PZ} insertion in the "l(2)0349703497" allele maps 164bp upstream of the 5' end of the CT4876 transcript of "wun". (Misra, 2000.8.25)
Other Comments
	wun2 functions in germ cells in the embryo, enabling them to perceive the wun/wun2-related signal from the soma. (Renault et al., 2004) wun and wun2 appear to act redundantly. (Starz-Gaiano et al., 2001) wun has two properties that allow it to use repulsion to guide the germ cells. wun can transform a permissive cellular environment into a repulsive one, and is expressed in the gut in a pattern that guides germ cells towards the mesoderm. Its sequence suggests that it is involved in lipid metabolism. (Zhang et al., 1997) The wun gene is involved in guiding germ cell migration. (Matthews, 1996.2.2) wun is required for guidance of germ cell migration in early development. Loss of wun function disrupts the orientation of motion causing germ cells to disperse even though their normal target is well formed. (Zhang et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057845 NM_165674 NM_001259294 RefSeq (Proteins) • NP_477193 NP_724787 NP_001246223 DDBJ / EMBL / GenBank • AAC47449 AAD38570 AAF58942 AAL34392 AAM71066 AAN71484 AC008341 AF145595 AFH07978 AI062852 AI401851 AQ025894 AQ026117 AQ073398 AQ073721 AY046533 BI486929 BT001729 CW848181 CZ471901 CZ473592 CZ473687 U73822 Z71084 Entrez Gene - A searchable database of RefSeq genes. • 35966 UniProtKB/Swiss-Prot • Q9V576
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Phosphatidic acid phosphatase type 2/haloperoxidase (IPR000326) Phosphatidic acid phosphatase/chloroperoxidase, N-terminal (IPR016118) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH02203
Linkouts
	DPiM - DPiM, Drosophila Protein interaction Map • FBgn0016078 DroID - A comprehensive database of gene and protein interactions. • FBgn0016078 DRSC - Results from RNAi screens. • FBgn0016078 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016078 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8804-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0016078 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35966 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /sturtevant/wunen1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35966 modMine - Data generated by the modENCODE project. • FBgn0016078
Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	CG8804 (Kraut et al., 2001, Kraut et al., 2001, Morrison et al., 2000, Ni et al., 2010.12.1) l(2)k16806 (Zhang et al., 1996, Kozopas, 2003.3, Spradling et al., 1999, ) LPP-like (Garcia-Murillas et al., 2006) wun (Xavier-Neto, 2005, Dionne et al., 2006, Makridou et al., 2003, Garcia-Murillas et al., 2006, Rynes et al., 2012, Renault et al., 2004, Burnett and Howard, 2003, Garcia-Murillas et al., 2006, Renault et al., 2004, Sano et al., 2005, Montell, 2006, Renault and Lehmann, 2006, Arancio et al., 2010, Tao et al., 2007, Bao and Friedrich, 2009, Choksi et al., 2006, Kucherenko et al., 2008, Adryan et al., 2007, Xi et al., 2008, Renault et al., 2010, Georgiev et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Ile et al., 2012) Wun (Cronin et al., 2009) Wunen (Hayden and Howard, 1997)
Name Synonym	wunen (Zhang, 1996.10.10, Howard and Zhang, 1997, Zhang et al., 1996, Matthews, 1996.2.2, Gregory et al., 2007, Garcia-Murillas et al., 2006, Foglietti et al., 2006, Renault et al., 2004, Garcia-Murillas et al., 2006, Montell, 2006, Renault and Lehmann, 2006, Tao et al., 2007, Bao and Friedrich, 2009, Wawersik et al., 2005, Renault et al., 2010, Eddison et al., 2012) Wunen (Burnett and Howard, 2003, Sano et al., 2005, Siddiqui et al., 2012)
Secondary FlyBase IDs
	FBgn0017369
References ( 111 )
Generate a list of	All references relating to this gene ( 111 )
List References by type	Research paper  ( 42 ) Supplementary material  ( 8 ) Review  ( 19 ) Personal communication to FlyBase  ( 17 ) Abstract  ( 12 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 3 ) Conference report  ( 3 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 1 )
Recent research papers ( 8 )
	Pantoja et al., 2013, Development 140(1): 136--146 Genetic elevation of Sphingosine 1-phosphate suppresses dystrophic muscle phenotypes in Drosophila. [FBrf0220147] Eddison et al., 2012, PLoS ONE 7(12): e51684 A genetic screen for olfactory habituation mutations in Drosophila: analysis of novel foraging alleles and an underlying neural circuit. [FBrf0220425] Georgiev et al., 2012, J. Neurogenet. 26(3-4): 338--347 Identification of a suppressor of retinal degeneration in Drosophila photoreceptors. [FBrf0220084] Gilchrist et al., 2012, Genes Dev. 26(9): 933--944 Regulating the regulators: the pervasive effects of Pol II pausing on stimulus-responsive gene networks. [FBrf0218258] Ile et al., 2012, Development 139(14): 2535--2546 Wunen, a Drosophila lipid phosphate phosphatase, is required for septate junction-mediated barrier function. [FBrf0218686] Rynes et al., 2012, Mol. Cell. Biol. 32(19): 3949--3962 Activating transcription factor 3 regulates immune and metabolic homeostasis. [FBrf0219385] Siddiqui et al., 2012, Genome Biol. 13(2): R11 Genome-wide analysis of the maternal-to-zygotic transition in Drosophila primordial germ cells. [FBrf0218847] Horn et al., 2011, Nat. Methods 8(4): 341--346 Mapping of signaling networks through synthetic genetic interaction analysis by RNAi. [FBrf0213352] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...