FlyBase Gene Report: Dmel\Alp4

PHOSPHATASES - UNCLASSIFIED -: Phosphatases are enzymes that catalyse the removal of a phosphate group. The Unclassified Phosphatases gene group contains phosphatases do not fit within the subgroups defined by FlyBase.
ALKALINE PHOSPHATASES -: Alkaline phosphatases function best in alkaline environments, catalyzing the hydrolysis of monoesters of phosphoric acid and a transphosphorylation reaction in the presence of large concentrations of phosphate acceptors. With few exceptions, they are homodimeric enzymes and each catalytic site contains three metal ions, i.e., two Zn and one Mg, necessary for enzymatic activity. They have broad substrate specificity. (Adapted from PMID:18404473.)

Protein Function (UniProtKB)

Important role in neural and renal epithelial function.

(UniProt, Q24238)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Alp4 or the JBrowse view of Dmel\Alp4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Alp4-RA

FBtr0085733

NM_079862

2153

596

Alp4-RB

FBtr0085734

NM_170534

2021

502

Additional Transcript Data and Comments

Reported size (kB)

2.05 (northern blot)

(Kloss et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Alp4-PA

FBpp0085095

65.3

596

6.26

NP_524601

AAF57106

Alp4-PB

FBpp0085096

54.9

502

6.06

NP_733413

AAN14265

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

578 (aa)

(Yang et al., 2000)

Comments

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q24238)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q24238

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Alp4 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

alkaline phosphatase activity

inferred from sequence or structural similarity with HGNC:439

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000174527

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

epithelial fluid transport

inferred from mutant phenotype

(assigned by UniProt )

(Yang et al., 2000)

multicellular organism reproduction

inferred from high throughput expression pattern

(Mueller et al., 2005)

inferred from expression pattern

(Swanson et al., 2001)

nervous system development

inferred from expression pattern

(assigned by UniProt )

(Yang et al., 2000)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

dephosphorylation

inferred from sequence or structural similarity with HGNC:439

(FlyBase Curators, 2008--)

inferred from biological aspect of ancestor with PANTHER:PTN000174527

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

cell surface

inferred from sequence or structural similarity with HGNC:439

(FlyBase Curators, 2008--)

extracellular region

inferred from sequence model

(Swanson et al., 2001)

extracellular space

inferred from sequence model

(Mueller et al., 2005)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

Malpighian tubule

(Yang et al., 2000)

ellipsoid body

(Yang et al., 2000)

larval stage & adult stage

(Yang et al., 2000)

Additional Descriptive Data

Aph-4 transcript levels are highest in larvae and adults. Aph-4 transcripts are detected by in situ hybridization in ellipsoid bodies in adult brain and in Malpighian tubules.

(Yang et al., 2000)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{GawB}Alp4^c232

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

ureter

(Sozen et al., 1997)

Malpighian tubule | restricted

(Sozen et al., 1997)

ellipsoid body

(O'Dell et al., 1995)

adult stage

ring neuron of ellipsoid body | subset

(Zars et al., 2000)

ring neuron R3

(Flybrain Neuron Database et al., 2018.6.28, Chen et al., 2012, Young and Armstrong, 2010, Baker et al., 2007, Yang et al., 2000, Renn et al., 1999)

Malpighian tubule

(Yang et al., 2000)

ellipsoid body

(Yang et al., 2000, Connolly et al., 1996)

ring neuron R4 | distal | subset

(Renn et al., 1999)

ring neuron R4

(Baker et al., 2007, Yang et al., 2000)

ellipsoid body | restricted

(Young and Armstrong, 2010, Zars et al., 2000, Renn et al., 1999)

ureter

(Yang et al., 2000, Sozen et al., 1997)

large field neuron | subset

(Renn et al., 1999)

main segment of Malpighian tubule | restricted

(Sozen et al., 1997)

adult brain | restricted

(Connolly et al., 1996)

ring neuron R4d

(Flybrain Neuron Database et al., 2018.6.28, Chen et al., 2012, Young and Armstrong, 2010)

pupal stage P7 -- adult stage

ring neuron R3

(Young and Armstrong, 2010)

ring neuron R4d

(Young and Armstrong, 2010)

Reporter: P{GawB}Alp4^c507

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

ureter

(Sozen et al., 1997)

Malpighian tubule | restricted

(Sozen et al., 1997)

transitional segment of Malpighian tubule

(Sozen et al., 1997)

third instar larval stage

ring neuron R3

(Renn et al., 1999)

Reporter: P{PZ}Alp4⁰⁷⁰²⁸

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

ureter

(Yang et al., 2000)

Malpighian tubule

(Yang et al., 2000)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Alp4 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Alp4

View exonic expression by tissue for Dmel\Alp4

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0016123

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1462-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1462

Flygut - An atlas of the Drosophila adult midgut

FBgn0016123

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Alp4

Transgenic constructs containing regulatory region of Alp4

Deletions and Duplications ( 3 )

Not disrupted in

Df(3R)tll-e

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Disrupted in

Df(3R)Exel6260

(Ryder, 2004.4.26)

T(Y;3)R133

(BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

Alp4⁰⁷⁰²⁸

partially lethal - majority die

Alp4⁰⁷⁰²⁸

partially lethal - majority die | recessive

Alp4⁰⁷⁰²⁸

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Alp4^GD3137

viable, with Scer\GAL4^Mef2.PR

Alp4^GD3137

viable, with Scer\GAL4^pnr-MD237

Alp4^GD3137

viable, with Scer\GAL4^tin.CΔ4

Alp4^GD3137

Other Phenotypes

Allele

stress response defective | adult stage

Alp4⁰⁷⁰²⁸

visible | adult stage

Alp4⁰⁷⁰²⁸

Phenotype manifest in

Allele

adult Malpighian tubule

Alp4^c232

wing

Alp4⁰⁷⁰²⁸

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\ALPP

7 of 15

Yes

Hsap\ALPG

6 of 15

Hsap\ALPI

6 of 15

Hsap\ALPL

6 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Alpi

7 of 15

Yes

Mmus\Akp3

6 of 15

Mmus\Alpl

6 of 15

Mmus\Alppl2

6 of 15

Rattus norvegicus (Norway rat) (5)

Rnor\Alpi

7 of 13

Yes

Rnor\Alpg

6 of 13

Yes

Rnor\Alpl

6 of 13

Rnor\Alpp

5 of 13

Rnor\Akp3

4 of 13

Xenopus tropicalis (Western clawed frog) (3)

Xtro\alpi.1

5 of 12

Yes

Xtro\alpl

5 of 12

Yes

Xtro\alpi.2

1 of 12

Danio rerio (Zebrafish) (4)

Drer\alpi.2

6 of 15

Yes

Drer\alpl

6 of 15

Yes

Drer\alpi.1

5 of 15

Drer\alp3

3 of 15

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\PHO8

9 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\SPBC14F5.13c

7 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091906RF )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13119

Drosophila persimilis

Dper\GL13536

Drosophila willistoni

Dwil\GK22608

Drosophila virilis

Dvir\GJ10501

Drosophila mojavensis

Dmoj\GI22314

Drosophila grimshawi

Dgri\GH13970

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502O7 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17018174

Glossina morsitans

Tsetse fly

Gmor\GMOY004885

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11821

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000931

Anopheles darlingi

American malaria mosquito

Adar\ADAC008590

Anopheles gambiae

Malaria mosquito

Agam\AGAP010596

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W071J )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA011462

Bombyx mori

Silkmoth

Bmor\BGIBMGA014304

Bombyx mori

Silkmoth

Bmor\BGIBMGA009872

Bombyx mori

Silkmoth

Bmor\BGIBMGA008818

Bombyx mori

Silkmoth

Bmor\BGIBMGA011463

Bombyx mori

Silkmoth

Bmor\BGIBMGA008401

Bombyx mori

Silkmoth

Bmor\BGIBMGA008824

Bombyx mori

Silkmoth

Bmor\BGIBMGA009866

Danaus plexippus

Monarch butterfly

Dple\DPOGS200818-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS210009-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS200817-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS215259-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS209233-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS215263-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS215432-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL016348

Heliconius melpomene

Postman butterfly

Hmel\HMEL010183

Heliconius melpomene

Postman butterfly

Hmel\HMEL016167

Heliconius melpomene

Postman butterfly

Hmel\HMEL017513

Heliconius melpomene

Postman butterfly

Hmel\HMEL013752

Heliconius melpomene

Postman butterfly

Hmel\HMEL013037

Apis florea

Little honeybee

Aflo\2322783764

Apis florea

Little honeybee

Aflo\2216906772

Apis florea

Little honeybee

Aflo\3446728468

Apis mellifera

Western honey bee

Amel\GB55499

Apis mellifera

Western honey bee

Amel\GB52063

Apis mellifera

Western honey bee

Amel\LOC410530

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP20243

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP19667

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP16694

Bombus terrestris

Buff-tailed bumblebee

Bter\15206170

Bombus terrestris

Buff-tailed bumblebee

Bter\15213365

Bombus terrestris

Buff-tailed bumblebee

Bter\15214776

Linepithema humile

Argentine ant

Lhum\LH11167

Linepithema humile

Argentine ant

Lhum\LH20731

Linepithema humile

Argentine ant

Lhum\LH15311

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001b20

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000a95

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001184

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG001233

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004816

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_07524

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_04596

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_01036

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02703

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05624

Tribolium castaneum

Red flour beetle

Tcas\TC032020

Tribolium castaneum

Red flour beetle

Tcas\TC034155

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_07539

Pediculus humanus

Human body louse

Phum\PHUM193940

Pediculus humanus

Human body louse

Phum\PHUM129520

Pediculus humanus

Human body louse

Phum\PHUM251300

Pediculus humanus

Human body louse

Phum\PHUM129510

Rhodnius prolixus

Kissing bug

Rpro\RPRC001092

Rhodnius prolixus

Kissing bug

Rpro\RPRC014817

Rhodnius prolixus

Kissing bug

Rpro\RPRC001113

Rhodnius prolixus

Kissing bug

Rpro\RPRC009958

Rhodnius prolixus

Kissing bug

Rpro\RPRC006863

Cimex lectularius

Bed bug

Clec\CLEC003059

Cimex lectularius

Bed bug

Clec\CLEC008087

Cimex lectularius

Bed bug

Clec\CLEC004940

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009772

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI007899

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI39787

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001572

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI063759

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006024

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004083

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006455

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11692

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_01430

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11690

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05461

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_05460

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06WR )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR007320

Ixodes scapularis

Black-legged tick

Isca\ISCW023785

Ixodes scapularis

Black-legged tick

Isca\ISCW000162

Stegodyphus mimosarum

African social velvet spider

Smim\KFM57384.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58295.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM59700.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56639.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM70846.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM58294.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur05g00330

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_251266

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_194087

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_269431

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_328304

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_228306

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_319945

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G067H )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Alpl_2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016431gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_016433gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Alpl_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Alpl

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Alpi

Ciona intestinalis

Vase tunicate

Cint\LOC100175777

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000021280

Ciona intestinalis

Vase tunicate

Cint\LOC100178630

Ciona intestinalis

Vase tunicate

Cint\ciap

Ciona intestinalis

Vase tunicate

Cint\LOC101243300

Ciona intestinalis

Vase tunicate

Cint\LOC100176308

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000023866

Gallus gallus

Domestic chicken

Ggal\LOC100859876

Gallus gallus

Domestic chicken

Ggal\ALPL

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (12)

Gene Symbol

Score

Source

Alignment

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

6 of 10

phu

6 of 10

5 of 10

5 of 10

5 of 10

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ALPP; alkaline phosphatase, placental

7 of 15

171800

ALPG; alkaline phosphatase, germ cell

6 of 15

171810

ALPI; alkaline phosphatase, intestinal

6 of 15

171740

ALPL; alkaline phosphatase, biomineralization associated

6 of 15

171760

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Homodimer.

(UniProt, Q24238 )

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0016123

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43671

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-102

Cytogenetic map

100B1-100B1

Sequence location

3R:31,048,929..31,052,540 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

100B1-100B1

Limits computationally determined from genome sequence between P{PZ}Aph-4⁰⁷⁰²⁸&P{PZ}dco^rK215 and P{PZ}l(3)rM731^rM731

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

100B2-100B2

(determined by in situ hybridisation) 100B (determined by in situ hybridisation)

(Yang et al., 2000)

100B4-100B5

(Yang, 1996.6.8)

100B1-100B2

(determined by in situ hybridisation)

(Spradling et al., 1999)

100B1-100B2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-102

(FlyBase, 2016)

3-98.9

(Comeron et al., 2012)

(Yang, 1996.6.8)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Bloomington

30828

w^*; P{GawB}Alp4^c232

30840

w^*; P{GawB}Alp4^c507

12285

P{PZ}Alp4⁰⁷⁰²⁸, ry⁵⁰⁶

28740

y¹ v¹; P{TRiP.JF03168}attP2

33673

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00537}attP2

VDRC

v110043

P{KK103971}VIE-260B

v6864

w¹¹¹⁸ P{GD3137}v6864

v6862

w¹¹¹⁸; P{GD3137}v6862

Genomic Clones (14)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (26)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

FI04434

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016123

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016123

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43671

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Aph-4 CG1462

(Yao, 1999.11)

Source for identity of: Alp4 Aph-4

(Marygold, 2015.6.26)

Source for database merge of

Source for merge of: Aph-4 l(3)07028

(Yang et al., 2000)

Additional comments

Renamed from 'Aph-4' to Alp4' to be consistent with the nomenclature of other named Dmel genes encoding Alkaline phosphatases and the mammalian nomenclature.

(Marygold, 2015.6.26)

Other Comments

Isolated in a screen for seminal fluid proteins.

(Mueller et al., 2004)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 42 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43671

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q24238

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016123

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1462

Flygut - An atlas of the Drosophila adult midgut

FBgn0016123

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

43671

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1462

modMine - A data warehouse for the modENCODE project

FBgn0016123

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0016123

DRSC - Results frm RNAi screens

FBgn0016123

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0016123

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1462-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016123

FlyMine - An integrated database for Drosophila genomics

FBgn0016123

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

43671

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Alp4

(Marygold, 2015.6.26)

Aph-4

(Zang et al., 2015, Ito et al., 2014, Attrill and Antonazzo, 2013.6.25, Japanese National Institute of Genetics, 2012.5.21, Kong et al., 2010, Takemori and Yamamoto, 2009, Ranade et al., 2008, Ravi Ram and Wolfner, 2007)

CG1462

(Çiçek et al., 2016, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009, Mueller et al., 2005, Mueller et al., 2004)

aph-4

(Berezikov et al., 2011)

l(3)07028

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

pMY51

(Yang et al., 2000)

Name Synonyms

Alkaline phosphatase

(Radyuk et al., 2012)

Alkaline phosphatase 4

(Takemori and Yamamoto, 2009, Rollmann et al., 2008, Harbison et al., 2005)

Alkaline phosphatase-4

(Landis et al., 2004)

alkaline phosphatase

(Yang, 1996.6.8)

Secondary FlyBase IDs

FBgn0010913

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (61)

Report Sections

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