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Gene Dmel\Cdk4

General Information
Symbol	Dmel\Cdk4	Species	D. melanogaster
Name	Cyclin-dependent kinase 4	Annotation symbol	CG5072
Feature type	protein_coding_gene	FlyBase ID	FBgn0016131
Gene Model Status	Current	Stock availability	26 publicly available
Also Known As	Cdk4/6, l(2)05428, dCdk4
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	53C9-53C9	Sequence location	2R:12,466,975..12,472,953 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Cyclin-dependent kinase 4 is referred to in FlyBase by the symbol Dmel\Cdk4 (CG5072, FBgn0016131). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: cyclin binding. There is experimental evidence that it is involved in the biological process: blastoderm segmentation; cell proliferation; cell growth; JAK-STAT cascade; cell cycle; mitochondrion organization; open tracheal system development. 36 alleles are reported. The phenotypes of these alleles are annotated with: embryonic/first instar larval cuticle; adult; abdominal 4 ventral denticle belt; cell cycle; mesothoracic tergum; wing; embryo; denticle belt. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 06-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, larval trachea, adult ovary, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1639347_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Cdk4. Gene sequence location is 2R:12466975..12472953.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 53C9-53C9   Limits computationally determined from genome sequence between P{PZ}Sema-2a03021&P{PZ}Cdk405428 and P{EP}BEST:SD02913EP2148&P{lacW}Dekk09907
Experimentally Determined Cytological Location
Cytogenetic map Notes References 53C-53C     (Lehner, 1996.7.23) 53C9-53C10   (determined by in situ hybridisation) 53C14--15 (determined by in situ hybridisation)   (Spradling et al., 1999) 53C9-53C10   (determined by in situ hybridisation)   (Perrimon et al., 1996) 53C-53C   (determined by in situ hybridisation)   (Sauer et al., 1996) 53C9-53C10   53C14--15   (BDGP Project Members, 1994-1999) 53C-53C   (determined by in situ hybridisation)   (Kalderon and Rubin, 1988)
Experimentally Determined Recombination Data
Location	2- (Lehner, 1996.7.23)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Cdk4 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Annotated transcripts do not represent all supported alternative splices within 5' UTR. Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Cdk4-RA FBtr0087103  NM_166184   1801   317 Cdk4-RB FBtr0087104  NM_057848   2026   317 Cdk4-RC FBtr0087102  NM_166185   1502   317 Cdk4-RD FBtr0340063     1447   317
Additional Transcript Data & Comments
Reported size (kB)	1.2 (northern blot) (Sauer et al., 1996)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Cdk4-PA   FBpp0086250   36.4   317   8.46     NP_725594   AAF57980 Cdk4-PB   FBpp0086251   36.4   317   8.46     NP_477196   AAF57981 Cdk4-PC   FBpp0086249   36.4   317   8.46     NP_725595   AAM68505 Cdk4-PD   FBpp0309069   36.4   317   8.46
Additional Polypeptide Data & Comments
Reported size (kDa)	317 (aa) (Sauer et al., 1996)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246773   AA696164   AA696227   AA696522   AA696524   AA803843   AA821187   AA950849   AA950999   AA978713   AA979228   AE013599 AAF57980   AE013599 AAF57981   AE013599 AAM68505   AC005647   AI518782   AQ024975   AQ025637   AQ025797   AQ073382   AQ073523   AW943070   AY060397 AAL25436   BE978648   BG636118   BG636717   BH900911   BI170545   BI242773   BI363050   BI372046   BT124771   CL705845   CO188050   CO266151   CO281938   CO331159   CZ472576   CZ474956   CZ481563   CZ489304   EC051999   EC053526   EC055974   EC056554   EC211155   EC233502   EC234281   EC237429   EC241080   EL873058   EL873144   EL873145   EL873322   EV580726   GH750923   GH751029   GH754946   GH756504   GH757868   GH757956   GH757977   GH759104   GH759389   GH772154   GH774098   GH774985   GH776837   GH777179   GH777347   GH780271   GH780308   GH781272   GH784358   GH785728   GH785748   GH786785   GH787671   GH787890   GH790367   GH793308   GH793496   GH797025   GH797117   GH798893   GH799170   GH799943   GH801212   GH802494   GH803571   GH804449   GH805801   GH806183   GH806458   GH807006   GH807612   GH809205   GH809495   GH810030   GH811533   GH811777   GH812464   GH812486   GH813423   GH813712   GH814178   GH815646   GH817014   GH819055   GH821071   GH824000   GH825152   GH825968   GH826853   GH827778   GH828042   GH829308   GH831330   GH831624   GH832254   GH833409   GH833593   GH835490   GH836099   GH836375   GH837031   GH837105   GH838197   GH838217   GH839250   GH840563   GH840742   GH841108   GH841734   GH842537   GH843120   GH843886   GH846646   GH847805   GH848149   GH848791   GH850658   GH851770   GH851907   GH853625   GH854339   GH855120   GH856689   GH856911   GH856921   GH857596   GH857673   GH857829   GH857948   GH858719   GH859624   GH859637   GH861086   GH862332   GH862708   GH866501   GH867779   GH868408   GH868987   GH869061   GH869381   GH870482   GH871407   GH872086   GH872861   GH874357   GH876547   GH877693   GH878816   GH879107   GH880952   GH880972   GH881188   GH882805   GH884346   GH885268   GH887249   GH888974   GH890709   GH891074   GH891190   GH893464   GH895680   GH897300   GH897614   GH897652   GH898265   GH898851   GH899298   GH900286   GH900753   GH904174   GH904492   GH906509   GH907117   GH907709   GH908169   GH908524   GH910474   GH910606   GH911249   GH912181   GH912786   GH914543   GH915769   GH919047   GH919734   GH922849   GH924294   GH924998   GH925092   GH925989   GH927208   GH927261   GH928327   GH930049   GH931009   GH932551   GH932766   GH935081   GH936734   GH937929   GH939216   GH946556   GH948640   GH958222   GH966704   GH967656   GH967721   GH970815   GH970874   GH971275   GH972248   GH974340   GH975669   GH978252   GH984320   X99510 CAA67860
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7K306 Q94877
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Sauer et al., 1996)
Additional Descriptive Data
	Cdk4 transcripts are detected at all stages of development on northern blots. They are found to be ubiquitous in early embryos by in situ hybridization. They are also present at a low level throughout the embryo at later stages of embryogenesis with stronger signals observed in internal tissues of germ band retracted embryos in a pattern correlated with endoreduplicaiton in these tissues. (Sauer et al., 1996)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, larval trachea, adult ovary, larval carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    115.925 Larval Midgut    64.6 Larval Hindgut    87.7 Larval Malpighian Tubules    27 Larval Fat Body    9 Larval Salivary Gland    112.6 Larval Trachea    152.275 Larval Carcass    127.125 Adult Head    69.6 Adult Eye    51.925 Adult Brain    17.1 Adult Thoracic-Abdominal Ganglion    25.3 Adult Crop    40.6 Adult Midgut    44.1 Adult Hindgut    29.9 Adult Malpighian Tubules    12.9 Adult Fat Body    53.9 Adult Salivary Gland    15.2 Adult Heart    23.35 Adult VirginFemale Spermatheca    22.8 Adult InseminatedFemale Spermatheca    31.7 Adult Ovary    239.1 Adult Testis    8.9 Adult Male Accessory Gland    15.4 Adult Carcass    47.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    69.6   NA  Eye    51.925   NA  Brain    no informative data   115.925  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    40.6   64.6  Midgut    44.1   87.7  Hindgut    29.9   27  Malpighian Tubules    no informative data   9  Fat Body    53.9   112.6  Salivary Gland    no informative data   NA  Heart    23.35   152.275  Trachea    NA   NA  VirginFemale Spermatheca    no informative data   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    239.1   NA  Testis    8.9   NA  Male Accessory Gland    no informative data   127.125  Carcass    47.8 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female  (30) ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female  30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    6 central nervous system, pupae P8    2 head, virgin 1-day female    6 head, virgin 4-day female    5 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    2 head, mated 1-day male    6 head, mated 4-day male    3 head, mated 20-day male    4 salivary gland, larvae L3 wandering    2 salivary gland, white prepupae    2 digestive system, larvae L3 wandering    2 digestive system, 1-day adult    3 digestive system, 4-day adult    3 digestive system, 20-day adult    2 fat body, larvae L3 wandering    1 fat body, white prepupae    2 fat body, pupae P8    3 carcass, larvae L3 wandering    11 carcass, 1-day adult    2 carcass, 4-day adult    2 carcass, 20-day adult    2 ovary, virgin 4-day female    30 ovary, mated 4-day female    22 testis, mated 4-day male    0 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 06-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    14 embryo 02-04hr    14 embryo 04-06hr    17 embryo 06-08hr    23 embryo 08-10hr    24 embryo 10-12hr    17 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    15 embryo 18-20hr    11 embryo 20-22hr    15 embryo 22-24hr    16 larva L1    14 larva L2    14 larva L3 12hr old    5 larva L3 puffstage 1-2    5 larva L3 puffstage 3-6    7 larva L3 puffstage 7-9    11 white prepupae new    10 white prepupae 12hr    12 white prepupae 24hr    13 pupae 2d postWPP    10 pupae 3d postWPP    6 pupae 4d postWPP    8 adult male 01day    4 adult male 05day    2 adult male 30day    2 adult female 01day    14 adult female 05day    16 adult female 30day    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1    26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8    26 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    33 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (39) Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1  26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8  26 wing disc ML-DmD9  26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21  (33) wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1  (29) CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2  (39) tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1    26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8    26 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    33 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1    26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8    26 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    33 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1    26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8    26 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    33 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (39) Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1  26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8  26 wing disc ML-DmD9  26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21  (33) wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1  29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2  (39) tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1    26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8    26 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    33 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    39 Schneider line 2 Sg4    21 embryonic 1182-4H    20 embryonic GM2    19 embryonic Kc167    8 embryonic S1    26 embryonic S3    21 leg disc CME L1    25 wing disc CME-W2    25 wing disc ML-DmD8    26 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    17 wing disc ML-DmD21    33 wing disc ML-DmD32    16 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    17 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    12 CNS ML-DmBG2-c2    39 tumorous blood cells mbn2    12 ovary fGS/OSS    14 ovary OSC    13 ovary OSS    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    5 cold shock, 4-day adult    4 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    8 Cadmium 50 mM 48 hrs, 4-day adult    8 Cadmium 100 mM 48 hrs, 4-day adult    15 Copper 0.5 mM 12 hrs, larvae L3    7 Copper 15 mM 48 hrs, 4-day adult    7 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    2 Ethanol 2.5% 3 hrs, larvae L3    18 Ethanol 5% 3 hrs, larvae L3    6 Ethanol 10% 3 hrs, larvae L3    6 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    13 Caffeine 25 mg/ml 48 hrs, 4-day adult    5 Paraquat 5 mM 48 hrs, 4-day adult    16 Paraquat 10 mM 48 hrs, 4-day adult    15 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016131 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5072-RC
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | maternal effect | poor Cdk405428 lethal | larval stage | recessive Cdk405428 lethal | maternal effect Cdk43 lethal | recessive Cdk405428 Cdk4k06503 Cdk4s4639 viable Cdk43 Cdk410 Cdk4EY09330 viable, with Scer\GAL4pnr-MD237 Cdk4GD11525 Sterility Allele fertile Cdk4EY09330 sterile | female | recessive Cdk43 Other Phenotypes Allele decreased cell number Cdk43 decreased cell number | recessive Cdk43 decreased cell number | somatic clone Cdk43 increased cell size | recessive Cdk43 radiation sensitive | recessive Cdk43 size defective Cdk43 size defective, with Scer\GAL4da.G32 Cdk4D175N.Scer\UAS.T:Hsap\MYC Cdk4K55M.Scer\UAS.T:Hsap\MYC small body Cdk43 small body | recessive Cdk43 visible, with Scer\GAL4pnr-MD237 Cdk4GD11525 Phenotype manifest in Allele abdominal 4 ventral denticle belt, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 Cdk4D175N.Scer\UAS.T:Hsap\MYC abdominal 4 ventral denticle belt | germline clone | maternal effect Cdk43 abdominal 5 ventral denticle belt | germline clone | maternal effect Cdk43 Cdk44 abdominal 6 ventral denticle belt | germline clone | rescuable maternal effect Cdk43 abdominal 7 ventral denticle belt | germline clone | rescuable maternal effect Cdk43 abdominal 8 ventral denticle belt | germline clone | rescuable maternal effect Cdk43 adult Cdk43 cell cycle Cdk43 denticle belt, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 Cdk4D175N.Scer\UAS.T:Hsap\MYC dorsal trunk primordium | germline clone | maternal effect Cdk43 embryo Cdk43 embryonic/first instar larval cuticle Cdk405428 embryonic/first instar larval cuticle | germline clone | rescuable maternal effect Cdk43 embryonic head | germline clone | rescuable maternal effect Cdk43 head | maternal effect Cdk405428 mesothoracic tergum, with Scer\GAL4pnr-MD237 Cdk4GD11525 segment | maternal effect Cdk405428 tracheal branch primordium | germline clone | maternal effect Cdk43 wing Cdk43
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of Cdk4 Class Mutagen Stocks Known lesion Cdk4s4639 P-element activity 3 Yes Cdk4k06503 P-element activity 2 Yes Cdk405428 P-element activity 1 Yes Cdk410 1 Yes Cdk43 P-element activity 1 Yes Cdk4CB-0588-3 1 -- Cdk4d02028 P-element activity 1 -- Cdk4d06166 P-element activity 1 -- Cdk4e03816 piggyBac transposase 1 -- Cdk4EY09330 P-element activity 1 -- Cdk4f06596 piggyBac transposase 1 -- Cdk4GS2301 1 Yes Cdk4KG07378 P-element activity 1 -- Cdk4NP0640 1 -- Cdk4NP2174 1 -- Cdk4NP3107 1 -- Cdk4NP6222 1 -- Cdk4NP6355 1 -- Cdk44 P-element activity 0 Yes Cdk45-SZ-3579 0 -- Cdk4EP2192 P-element activity 0 Yes Cdk4EP2358 P-element activity 0 Yes Cdk4EP844 P-element activity 0 Yes Cdk4unspecified 0 --
Alleles Carried on Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Cdk4 Class Mutagen Stocks Known lesion Cdk4GD11525 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Cdk4GL00240 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cdk4JF02795 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Cdk4Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion 1 Yes Cdk4cDa in vitro construct 0 Yes Cdk4cXa in vitro construct 0 Yes Cdk4D175N.Scer\UAS.T:Hsap\MYC in vitro construct - amino acid replacement 0 Yes Cdk4hs.PC in vitro construct - regulatory fusion 0 Yes Cdk4K55M.Scer\UAS.T:Hsap\MYC in vitro construct - amino acid replacement 0 Yes Cdk4MtnA.T:SV5\V5 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Cdk4NIG.5072R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Cdk4Scer\UAS.cMa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7144 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11525} NA P{NIG.5072R} NA P{TRiP.GL00240} NA P{TRiP.JF02795} NA P{UAS-Cdk4.M} NA P{UAS-Cdk4D175N-myc} NA P{UAS-Cdk4K55M-myc} NA P{UAS-Cdk4-myc} NA heat-shock construct P{hs-Cdk4.C} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}Cdk4EP844 NA P{EP}Cdk4EP2192 NA P{EP}Cdk4EP2358 NA P{EPgy2}Cdk4EY09330 NA P{GSV1}Cdk4GS2301 NA P{XP}Cdk4d02028 NA P{XP}Cdk4d06166 NA insertion of enhancer trap P{lacW}Cdk44 No P{lacW}Cdk4k06503 No P{lacW}Cdk4s4639 No P{lacW}l(2)SH0671SH0671 No P{PZ}Cdk405428 No miscellaneous insertions P{RS3}Cdk4CB-0588-3 NA P{RS5}Cdk45-SZ-3579 NA P{SUPor-P}Cdk4KG07378 NA P{UASp-YFP.Rab30}Cdk410 NA PBac{RB}Cdk4e03816 NA PBac{WH}Cdk4f06596 NA insertion of enhancer trap binary system P{GawB}Cdk4NP0640 No P{GawB}Cdk4NP2174 No P{GawB}Cdk4NP3107 No P{GawB}Cdk4NP6222 No P{GawB}Cdk4NP6355 No
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function
CV term Evidence References
cyclin binding inferred from physical interaction with CycD (Meyer et al., 2000, Sauer et al., 1996)
Biological Process
CV term Evidence References
blastoderm segmentation inferred from mutant phenotype (Chen et al., 2003) cell cycle inferred from mutant phenotype (Kiger et al., 2003) cell growth inferred from mutant phenotype (Meyer et al., 2000, Frei et al., 2005) cell proliferation inferred from genetic interaction with CycD (Datar et al., 2000) JAK-STAT cascade inferred from genetic interaction with hop (Chen et al., 2003) inferred from genetic interaction with os, CycD (Chen et al., 2003) inferred from mutant phenotype (Chen et al., 2003) inferred from physical interaction with Stat92E (Chen et al., 2003) mitochondrion organization inferred from mutant phenotype (Frei et al., 2005) open tracheal system development inferred from mutant phenotype (Chen et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) cyclin-dependent protein serine/threonine kinase activity inferred from sequence or structural similarity with human CDK4 (Sauer et al., 1996) inferred from sequence or structural similarity with Saccharomyces CDC28 (FlyBase, 1992-) protein serine/threonine kinase activity inferred from sequence or structural similarity (Kalderon and Rubin, 1988) non-traceable author statement (Lehner, 1996.7.23, Morrison et al., 2000)
Biological Process
CV term Evidence References
protein phosphorylation inferred from sequence or structural similarity (Kalderon and Rubin, 1988, Sauer et al., 1996) inferred from sequence or structural similarity with human CDK4 (Sauer et al., 1996) inferred from sequence or structural similarity with Saccharomyces CDC28 (FlyBase, 1992-) non-traceable author statement (Morrison et al., 2000) regulation of cell cycle traceable author statement (Johnston and Gallant, 2002) regulation of cell growth traceable author statement (Johnston and Gallant, 2002)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Cdk4 - Arpc2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - CG14786 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - CG3731 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - CG4612 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Cdc37 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Srp54 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - Syp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Cdk4 - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Cdk4 allele Gene References Cdk43 hop (Chen et al., 2003) Rbf (Meyer et al., 2000) Tsc1 (Tapon et al., 2001) Cdk4D175N.Scer\UAS.T:Hsap\MYC CycD (Datar et al., 2000) Cdk4hs.PC hop (Chen et al., 2003) Cdk4Scer\UAS.cMa CycD (Datar et al., 2000, Hipfner et al., 2002) Tsc1 (Tapon et al., 2001) Cdk4Scer\UAS.T:Hsap\MYC CycD (Chen et al., 2003) unspecified gig (Tapon et al., 2001) os (Mukherjee et al., 2006, Chen et al., 2003) pbl (Gregory et al., 2007) ptc (Duman-Scheel et al., 2002) Rbf (Datar et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62567 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0016131 DroID - A comprehensive database of gene and protein interactions. • FBgn0016131 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36854 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q7K306
Orthologs
OrthoDB Orthologs (43) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6QNNG7 Dipteran inclusive ortholog search EOG6K0QX4 Insect inclusive ortholog search EOG679D3M Arthropod inclusive ortholog search EOG68SF9B Metazoa inclusive ortholog search EOG6J48BJ
Orthologs in Drosophila Species (EOG6QNNG7)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Cdk4     Drosophila simulans   Dsim\GD11207 Y   Drosophila sechellia   Dsec\GM21710 Y   Drosophila erecta   Dere\GG20615 Y   Drosophila yakuba   Dyak\GE11805 Y   Drosophila ananassae   Dana\GF11396 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18638 Y   Drosophila persimilis   Dper\GL10885 Y   Drosophila willistoni   Dwil\GK19645 Y   Drosophila virilis   Dvir\GJ22324 Y   Drosophila mojavensis   Dmoj\GI20474 Y   Drosophila grimshawi   Dgri\GH21188 Y
Orthologs in non-Drosophila Dipterans (EOG6K0QX4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL001407   Anopheles gambiae Malaria mosquito  Agam\AGAP005817   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ015895
Orthologs in non-Dipteran Insects (EOG679D3M)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB48243   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG003353   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH12402   Atta cephalotes Leafcutter ant  Acep\ACEP24857   Camponotus floridanus Florida carpenter ant  Cflo\CFLO18646   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL21756   Linepithema humile Argentine ant  Lhum\LH21359   Pogonomyrmex barbatus Red harvester ant  Pbar\PB10192   Solenopsis invicta Red fire ant  Sinv\SINV24935   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005353   Bombyx mori Silkmoth  Bmor\BGIBMGA001344   Pediculus humanus Human body louse  Phum\PHUM065530   Tribolium castaneum Red flour beetle  Tcas\TC008616
Orthologs in non-Insect Arthropods (EOG68SF9B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_318234   Ixodes scapularis Deer tick  Isca\ISCW016926
Orthologs in non-Arthropod Metazoa (EOG6J48BJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\cdk-4   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000151gn   Danio rerio Zebrafish  Drer\si:ch211-234f14.1   Danio rerio Zebrafish  Drer\zgc:153726   Danio rerio Zebrafish  Drer\ENSDARG00000030163   Xenopus tropicalis Western clawed frog  Xtro\cdk6   Gallus gallus Domestic chicken  Ggal\cdk6   Mus musculus House mouse  Mmus\Cdk6   Mus musculus House mouse  Mmus\Cdk4   Rattus norvegicus Norway rat  Rnor\Cdk4   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000009258   Homo sapiens Human  Hsap\CDK4   Homo sapiens Human  Hsap\CDK6
Human Orthologs (2)
Gene OMIM HGNC Hsap\CDK4     Hsap\CDK6
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11207 Drosophila sechellia Dsec\GM21710 Drosophila erecta Dere\GG20615 Drosophila yakuba Dyak\GE11805 Drosophila ananassae Dana\GF11396 Drosophila pseudoobscura pseudoobscura Dpse\GA18638 Drosophila persimilis Dper\GL10885 Drosophila willistoni Dwil\GK19645 Drosophila virilis Dvir\GJ22324 Drosophila mojavensis Dmoj\GI20474 Drosophila grimshawi Dgri\GH21188
Stocks & Reagents
Stocks Listed in FlyBase ( 26 )
Bloomington	6631 w*; P{UAS-Cdk4-myc}II.1
Harvard	- P{XP}Cdk4d02028 - P{XP}Cdk4d06166 - PBac{RB}Cdk4e03816 - PBac{WH}Cdk4f06596
Kyoto	113106 w*; P{GawB}Cdk4NP3107 123247 w1118; P{RS3}Cdk4CB-0588-3 103688 w*; P{GawB}Cdk4NP0640 / CyO 108880 w1118; P{lacW}Cdk4s4639/CyO 112810 w*; P{GawB}Cdk4NP2174 / CyO
VDRC	v40576 w1118; P{GD11525}v40576 v40577 w1118; P{GD11525}v40577
	More stocks available...
Genomic Clones ( 1 )
	BACR36P23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 219 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD31205
Other clones	DPiM_LD31205 MIP21741
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM05206 GM05331 GM07828 GM07830 GM14419 GM14822 LD30994 LD32713 LD33563 LD38511 RE12049 RE40192 RE47765 RE57550 SD13716 SD14486
Other clones	bs81c02 Dmel_dig_012200_0589_1967 EC051999 EC053526 EC055974 EC056554 EK036816 EK145110 EK159133 EK298661 IP04863 IP04963 IP05163 RP003004615 RP003008151 RP003008799 RP003019563 RP003025078 RP003028309 RP003030789 RP003031658 RP003034821 RP003041365 RP003050213 RP003058875 RP003061352 RP003062967 RP003066849 RP003069636 RP003079132 RP003083931 RP003084135 RP003094207 RP003096319 RP003102552 RP003102738 RP003103222 RP003103980 RP003106301 RP003109973 RP003111711 RP003112130 RP003112942 RP003113972 RP003119050 RP003120106 RP003120867 RP003123822 RP003124247 RP003129860 RP003132743 RP003136265 RP003137946 RP003139095 RP003140148 RP003140938 RP003141943 RP003145038 RP003156167 RP003164884 RP003165059 RP003169268 RP003179816 RP003180338 RP003180607 RP003180844 RP003187039 RP003200601 RP003201546 RP003206084 RP003212439 RP003212882 RP003213313 RP003215280 RP003215574 RP003226982 RP003229702 RP003234610 RP003239903 RP003240243 RP003243074 RP003244679 RP003250627 RP003253056 RP003257295 RP003267399 RP003269244 RP003271705 RP003273143 RP003278700 RP003282152 RP003287140 RP003288358 RP003289432 RP003294039 RP003294409 RP003296616 RP003296731 RP003301111 RP003303223 RP003315925 RP003320620 RP003320931 RP003321040 RP003324826 RP003327706 RP003328339 RP003344704 RP003345502 RP003348147 RP003355359 RP003357623 RP003366540 RP003376181 RP003382277 RP003383346 RP003383841 RP003388264 RP003396639 RP003397625 RP003402206 RP003404371 RP003408226 RP003410738 RP003420548 RP003422006 RP003422989 RP003423210 RP003425508 RP003432095 RP003435178 RP003443076 RP003444536 RP003445031 RP003446804 RP003447483 RP003453759 RP003462161 RP003462214 RP003466913 RP003477365 RP003481245 RP003483193 RP003483470 RP003483710 RP003484198 RP003490174 RP003493516 RP003495494 RP003509069 RP003511248 RP003529514 RP003529969 RP003530689 RP003530735 RP003535765 RP003546069 RP003550547 RP003551803 RP003552512 RP003561299 RP003565950 RP003575072 RP003576553 RP003580122 RP003582974 RP003585154 RP003589529 RP003591369 RP003591880 RP003612218 RP003613164 RP003613565 RP003617239 RP003623554 RP003627086 RP003649083 RP003650371 RP003651813 RP003655925 RP003661025 RP003663698 RP003673698 RP003675265 RP003680268 RP003680965 RP004039578 RP004196705 RP004200054 RP004219208 RP004261903 RP004337477 RP004365610 RP004400141 RP004414729 RP004420833 RP004421570 RP004463709 RP004465285 RP004476605
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0016131 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36854
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Cdk4/6 CG5072 (Li, 1999.11)
Source for database merge of	Source for merge of: Cdk4/6 l(2)05428 l(2)k06503 (Misra, 2000.8.25) Source for merge of: Cdk4 l(2)s4639 (Meyer et al., 2000) Source for merge of: Cdk4 l(2)SH0671 (Chen et al., 2003) Source for merge of: Cdk4 Pk53C (Bayraktaroglu, 2000.11.2)
Additional comments
Other Comments
	When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an decrease, an increase in the proportion of S phase cells, an increase in cell size, in the proportion of G2/M phase cells and spindle abnormalities (branched spindles) are seen. (Bettencourt-Dias et al., 2004) The apparently indistinguishable phenotypes of CycD Cdk4 double mutants and either CycD or Cdk4 single mutant argue against major independent functions of CycD and Cdk4 in D.melanogaster. (Emmerich et al., 2004) Mutations in dm and Cdk4 do not induce autophagy in the fat body. (Scott et al., 2004) Cdk4 is a component of the hop/Stat92E signal transduction pathway. (Chen et al., 2003) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cell size is increased, microtubules are uniform or disorganised, cell shape is irregular, and cell number is decreased indicative of a failure in cell cycle progression through G1 to S and G2 to M stages. This phenotype is not seen in Kc167 cells. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) CycD-Cdk4 is not a master regulator of the cell embryonic cell proliferation program. (Meyer et al., 2002) The primary function of CycD-Cdk4 is to stimulate growth. (Datar et al., 2000) Cdk4 has a role in the modulation of growth rates and is dispensable for cell cycle progression. (Meyer et al., 2000) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. (Perrimon et al., 1996) Characterisation of Cdk4. (Sauer et al., 1996) Isolated from a genomic library using a Pka-C1 probe. (Kalderon and Rubin, 1988) Cdk4 may encode a protein kinase. (Kalderon and Rubin, 1988) Gene in D.melanogaster encoding product related (by sequence comparison) to the serine-threonine protein kinases of mammals. Isolated from Drosophila clones obtained with mammalian probes.
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057848 NM_166184 NM_166185 RefSeq (Proteins) • NP_477196 NP_725594 NP_725595 DDBJ / EMBL / GenBank • AA246773 AA950999 AAF57980 AAF57981 AAL25436 AAM68505 AC005647 AQ024975 AQ025637 AQ025797 AQ073382 AQ073523 AW943070 AY060397 BH900911 CAA67860 CL705845 CZ472576 CZ474956 CZ481563 CZ489304 X99510 Entrez Gene - A searchable database of RefSeq genes. • 36854 UniProtKB/TrEMBL • Q7K306 Q94877
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62567 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0016131 DroID - A comprehensive database of gene and protein interactions. • FBgn0016131 DRSC - Results from RNAi screens. • FBgn0016131 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016131 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5072-RC FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0016131 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36854 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/cdk4-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36854 modMine - Data generated by the modENCODE project. • FBgn0016131 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q7K306
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	8-6 (Kalderon and Rubin, 1988, ) cdk (Parrish et al., 2009) cdk4 (Scott et al., 2004, Scott et al., 2004, Moberg and Hariharan, 2003, Ren et al., 2004, Tapon et al., 2001, Frolov et al., 2003, Orian et al., 2003, Silver and Montell, 2003, Frolov et al., 2003, Hipfner et al., 2002, Duman-Scheel et al., 2002, Tapon et al., 2001, Datar and Edgar, 1999, Jaklevic et al., 2006, Gregory et al., 2007, Herr et al., 2007, Sandmann et al., 2007, Lu et al., 2007, Smith-Bolton et al., 2009, Nicholson et al., 2009, Morris et al., 2006, Genevet et al., 2009, Flannery et al., 2010, Djiane et al., 2011, Sasai et al., 2007, Datar et al., 2006, Gupta et al., 2008) CDK4 (Roy and Ingham, 2002, Saucedo et al., 2001, Saucedo et al., 2000, Edgar, 2006, Bakal et al., 2008, Faradji et al., 2011) Cdk4 (Nolo et al., 2006, Chen et al., 2003, Mukherjee et al., 2006, Loren et al., 2003, Baltzer et al., 2007, Friedman and Perrimon, 2006, Gilboa and Lehmann, 2006, Berry and Baehrecke, 2007, Zhang et al., 2007, Secombe et al., 2007, Buttitta et al., 2007, Layalle et al., 2008, Rafel and Milán, 2008, Hamaratoglu et al., 2009, Tanaka-Matakatsu et al., 2007, Parrish et al., 2009, Khurana et al., 2006, Baltzer et al., 2009, Reddy et al., 2010, Beltran et al., 2007, Coffman et al., 2005, Ferjoux et al., 2007, Japanese National Institute of Genetics, 2012.5.21, Gurudatta et al., 2012) Cdk4/6 (Bjorklund et al., 2006, Loren et al., 2001, Tseng et al., 2007) cdk4/6 (Asha et al., 2003, Lehner, 1996.7.23, Sauer et al., 1996) CDK4/6 (Morrison et al., 2000) CG5072 (Morrison et al., 2000, Perkins et al., 2009, Ni et al., 2011.7.19) dCdk4 (Ji et al., 2012, Read et al., 2009) DmCdk4 (Kolonin and Finley, 1998) l(2)0671 (Chen et al., 2003) l(2)05428 (Spradling et al., 1999, Perrimon et al., 1996, ) l(2)k06503 (Spradling et al., 1999, ) l(2)s4639 (Spradling et al., 1999, ) l(2)sh0671 (Chen et al., 2003) Pk53C   Pk?7
Name Synonym	Cyclin-dependent kinase 4 (Mukherjee et al., 2006) cyclin-dependent kinase 4 (Genevet et al., 2009) Cyclin-dependent kinase 4/6   Protein kinase-like 53C
Secondary FlyBase IDs
	FBgn0004458 FBgn0010600 FBgn0010696 FBgn0022125
References ( 136 )
Generate a list of	All references relating to this gene ( 136 )
List References by type	Research paper  ( 72 ) Supplementary material  ( 11 ) Review  ( 16 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 16 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) Book  ( 1 )
Recent research papers ( 6 )
	Baker, 2013, PLoS ONE 8(3): e58266 Developmental Regulation of Nucleolus Size during Drosophila Eye Differentiation. [FBrf0220989] Gurudatta et al., 2012, Dev. Biol. 369(1): 124--132 The BEAF insulator regulates genes involved in cell polarity and neoplastic growth. [FBrf0219796] Icreverzi et al., 2012, Cell Cycle 11(3): 554--568 Drosophila cyclin D/Cdk4 regulates mitochondrial biogenesis and aging and sensitizes animals to hypoxic stress. [FBrf0217471] Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Djiane et al., 2011, J. Cell Biol. 192(1): 189--200 Su(dx) E3 ubiquitin ligase-dependent and -independent functions of Polychaetoid, the Drosophila ZO-1 homologue. [FBrf0212737] Faradji et al., 2011, Cell Cycle 10(5): 805--818 Drosophila melanogaster Cyclin G coordinates cell growth and cell proliferation. [FBrf0213161] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...