Pdp1ε, pdp, Pdp-1, Pdp1&e;gr, Par domain protein 1ε
PAR-domain bZIP transcription factor - controls skeletal muscle genes during differentiation - regulates expression of the Tropomyosin I gene in somatic body-wall and pharyngeal muscles
Low-frequency RNA-Seq exon junction(s) not annotated.
Stage-specific extension of 3' UTRs observed during embryogenesis (FBrf0215804); all variants may not be annotated.
Gene model reviewed during 5.45
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.46
Gene model reviewed during 5.56
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pdp1 using the Feature Mapper tool.
Expression oscillates with the circadian cycle and peaks at ZT16, with the peak becoming less pronounced as flies age. The relative amount of mRNA is less in bodies compared to heads.
The Pdp1 isoforms Pdp1-RD and RJ, collectively referred in this paper as Pdp1-ε isoform, are detected in the heads of adult flies by RT-PCR. In conditions of constant darkness, the levels of both isoforms cycle, increasing gradually from CT02 until CT14, and decreasing after that until CT20.
The Pdp1 isoforms Pdp1-PD and PD, collectively referred in this paper as Pdp1-ε isoform, are detected in head extracts of adult flies by western blot. The isoform Pdp1-PJ is the predominant one. Both isoforms show a cycling behaviour, with levels gradually increasing from CT02 until CT18, and decrease after that until ZT22. Immunostaining reveals that at ZT20, Pdp1 protein is expressed in the dorsal and ventral lateral clock neurons (LNd and LNv) in the adult brain.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Pdp1 in GBrowse 2
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Pdp1 CG3129
Source for merge of Pdp1 anon-WO0140519.206 was sequence comparison ( date:051113 ).
Pdp1 is sensitive to nutritional conditions, with two of its protein isoforms becoming upregulated in response to food withdrawal.
Identified as a gene with significant level of mRNA cycling as assessed by expression analysis using high density oligonucleotide arrays with probe generated from adult heads harvested over six time points over the course of a day. Showed cycling in Canton S and low expression in Clk mutants in microarray experiments. Identified in S2/cycloheximide assay as a direct target of Clk mediated transcriptional regulation.
Pdp1 is involved in regulating expression of Tm2 gene in the somatic body wall and pharyngeal muscles by binding to DNA sequences within the muscle activator that are required for activator function. Results suggest Pdp1 may function as part of a large protein/DNA complex that interacts with Mef2 to regulate muscle gene transcription. Pdp1 may also be involved in terminal differentiation of the midgut endoderm, hindgut and Malpighian tubules, the epidermis and the central nervous system.