FlyBase Gene Report: Dmel\Prosα5

General Information

Symbol

Dmel\Prosα5

Species

D. melanogaster

Name

Proteasome α5 subunit

Annotation Symbol

CG10938

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016697

Gene Model Status

Current

Stock Availability

7 publicly available

Gene Snapshot

Contributions welcome

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

ProsMA5, α5, PSMA5, alpha5

Key Links

Genomic Location

Cytogenetic map

54B7-54B7

Sequence location

2R:17,412,767..17,413,769 [+]

Recombination map

2-82

RefSeq locus

NT_033778 REGION:17412767..17413769

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (9 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables endopeptidase activity

inferred from biological aspect of ancestor with PANTHER:PTN000174701

(assigned by GO_Central )

(Gaudet et al., 2011)

enables threonine-type endopeptidase activity

inferred from electronic annotation with InterPro:IPR001353, InterPro:IPR023332

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Lőw et al., 2013, Wojcik and DeMartino, 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in proteasomal ubiquitin-independent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000174701

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000174701

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred by curator from GO:0005839

(Sumegi et al., 2003)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of proteasome complex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome core complex

inferred from direct assay

(Sumegi et al., 2003)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000174701

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000174701

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of proteasome core complex, alpha-subunit complex

inferred from sequence or structural similarity with UniProtKB:P40302

(assigned by UniProt )

(UniProt Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN000175163

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of proteasome core complex

inferred from biological aspect of ancestor with PANTHER:PTN000174701

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

PROTEASOME ALPHA SUBUNITS

Protein Family (UniProt)

Belongs to the peptidase T1A family. (Q95083)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

PROTEASOME ALPHA SUBUNITS -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. The core particle is a highly conserved, barrel-shaped complex composed of four stacked heptameric rings. Narrow substrate entry channels are created by the two outer rings, which are each formed by seven alpha subunits. (Adapted from FBrf0215459 and PMID:28583440).

Protein Function (UniProtKB)

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

(UniProt, Q95083)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Prosα5 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Prosα5-RA

FBtr0086910

NM_166221

884

244

Prosα5-RB

FBtr0086911

NM_057854

873

244

Additional Transcript Data and Comments

Reported size (kB)

0.9 (longest cDNA)

(Zaiss and Belote, 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Prosα5-PA

FBpp0086066

26.9

244

4.63

NP_725669

AAF57875

Prosα5-PB

FBpp0086067

26.9

244

4.63

NP_477202

AAM70874

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

244 aa isoforms: Prosα5-PA, Prosα5-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

244 (aa); 27 (kD predicted)

(Zaiss and Belote, 1997)

Comments

External Data

Subunit Structure (UniProtKB)

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity).

(UniProt, Q95083)

Crossreferences

InterPro - A database of protein families, domains and functional sites

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

T01.975

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Q95083

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Prosα5 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosalpha5_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

primordial germ cell

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

germline cell

(Fisher et al., 2012)

embryonic stage 11 -- 12

germline cell

(Fisher et al., 2012)

embryonic stage 13 -- 16

germline cell

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of proteasome complex

inferred from direct assay

(Nickell et al., 2009)

part_of proteasome core complex

inferred from direct assay

(Sumegi et al., 2003)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Prosα5 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Prosα5

View exonic expression by tissue for Dmel\Prosα5

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016697

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0016697

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10938-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10938

Flygut - An atlas of the Drosophila adult midgut

FBgn0016697

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Prosα5

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 3 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Prosα5

Transgenic constructs containing regulatory region of Prosα5

Deletions and Duplications ( 1 )

Disrupted in

Df(2R)Exel7147

(Ryder, 2004.4.26)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Prosα5^GD7134

lethal, with Scer\GAL4^Mef2.PR

Prosα5^GD7134

lethal, with Scer\GAL4^pnr-MD237

Prosα5^GD7134

lethal, with Scer\GAL4^tin.CΔ4

Prosα5^GD7134

Phenotype manifest in

Allele

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^Cg.PA

Prosα5^HMS00095

mitochondrion | third instar larval stage, with Scer\GAL4^Cg.PA

Prosα5^HMS00095

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (16)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PSMA5

14 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (17)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Psma5

14 of 15

Yes

Mmus\Gm8394

4 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (15)

Rnor\Psma5

13 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (15)

Xtro\psma5

8 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Danio rerio (Zebrafish) (20)

Drer\psma5

14 of 15

Yes

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (12)

Cele\pas-5

12 of 15

Yes

2 of 15

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (13)

Atha\PAE1

9 of 9

Yes

Atha\PAE2

8 of 9

Yes

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

1 of 9

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\PUP2

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pup2

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190E5P )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10654

Drosophila persimilis

Dper\GL11421

Drosophila willistoni

Dwil\GK21413

Drosophila virilis

Dvir\GJ20367

Drosophila mojavensis

Dmoj\GI18573

Drosophila grimshawi

Dgri\GH21393

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091509PH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17020944

Glossina morsitans

Tsetse fly

Gmor\GMOY008302

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_12234

Mayetiola destructor

Hessian fly

Mdes\Mdes003973

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL004308

Anopheles darlingi

American malaria mosquito

Adar\ADAC006583

Anopheles gambiae

Malaria mosquito

Agam\AGAP008816

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010114

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0ATC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA013898

Danaus plexippus

Monarch butterfly

Dple\DPOGS202375-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL002895

Apis florea

Little honeybee

Aflo\3901768628

Apis mellifera

Western honey bee

Amel\Prosalpha5

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11900

Bombus terrestris

Buff-tailed bumblebee

Bter\15216818

Linepithema humile

Argentine ant

Lhum\LH19065

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:001c8b

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005999

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05420

Tribolium castaneum

Red flour beetle

Tcas\TC008671

Pediculus humanus

Human body louse

Phum\PHUM116710

Rhodnius prolixus

Kissing bug

Rpro\RPRC006893

Cimex lectularius

Bed bug

Clec\CLEC004397

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI009032

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_12040

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0AQC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR014223

Ixodes scapularis

Black-legged tick

Isca\ISCW012814

Stegodyphus mimosarum

African social velvet spider

Smim\KFM77976.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur22g02630

Tetranychus urticae

Two-spotted spider mite

Turt\tetur63g00010

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_231717

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GX6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psma7

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psma5

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_018047gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psma2_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_023191gn

Ciona intestinalis

Vase tunicate

Cint\LOC100176760

Ciona intestinalis

Vase tunicate

Cint\LOC100179585

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000009332

Gallus gallus

Domestic chicken

Ggal\PSMA2

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000027156

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000000065

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (24)

Gene Symbol

Score

Source

Alignment

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

2 of 10

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMA5; proteasome 20S subunit alpha 5

14 of 15

176844

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Prosα5 - CG11885

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - CG12321

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - CG13319

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - GNBP2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Klc

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - PSMG1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Pomp

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosα1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosα2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosα3

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosα6

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosα7

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ3

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ4R2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ5

anti bait coimmunoprecipitation, western blot, experimental knowledge based

(Tsakiri et al., 2013, Tsakiri et al., 2013, Guruharsha et al., 2011)

Prosα5 - Prosβ6

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Prosβ7

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - REG

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn11

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn13

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn6

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn7

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn8

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpn9

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpt2

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpt3

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

Prosα5 - Rpt6

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, Q95083 )

Linkouts

BioGRID - A database of protein and genetic interactions.

62645

DroID - A comprehensive database of gene and protein interactions.

FBgn0016697

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36951

MIST (protein-protein) - An integrated Molecular Interaction Database

Prosalpha5

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-82

Cytogenetic map

54B7-54B7

Sequence location

2R:17,412,767..17,413,769 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

54B7-54B7

Limits computationally determined from genome sequence between P{EP}EP1061&P{EP}cnk^EP576 and P{EP}l(2)k01209^EP2177&P{lacW}k11012a

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

54B-54B

(Zaiss, 1996.7.22)

54B3-54B5

(determined by in situ hybridisation)

(Zaiss and Belote, 1997)

Experimentally Determined Recombination Data

Location

2-82

(FlyBase, 2016)

2-86.2

(Comeron et al., 2012)

(Zaiss, 1996.7.22)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

34786

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00095}attP2

FlyORF

F001354

M{UAS-Prosα5.ORF}ZH-86Fb

F000809

M{UAS-Prosα5.ORF.3xHA}ZH-86Fb

Kyoto

104356

y^* w^*; P{GawB}Prosα5^NP3047 / CyO, P{UAS-lacZ.UW14}UW14

VDRC

v108380

P{KK108214}VIE-260B

v16105

w¹¹¹⁸; P{GD7134}v16105

v16104

w¹¹¹⁸; P{GD7134}v16104/TM3

Genomic Clones (18)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (84)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD33318

Other clones

DPiM_LD33318

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016697

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016697

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36951

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Additional comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

Other Comments

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

ProsMA5 has been cloned and sequenced.

(Zaiss and Belote, 1997)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 90 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36951

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q95083

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016697

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016697

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Prosalpha5_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10938

Flygut - An atlas of the Drosophila adult midgut

FBgn0016697

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

99021

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36951

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC008671

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10938

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

T01.975

modMine - A data warehouse for the modENCODE project

FBgn0016697

Linkouts

ApoDroso - Functional genomic database for photoreceptor development, survival and function

12771-Pros_5BG02176

BioGRID - A database of protein and genetic interactions.

62645

DPiM - Drosophila Protein interaction map

FBgn0016697

DroID - A comprehensive database of gene and protein interactions.

FBgn0016697

DRSC - Results frm RNAi screens

FBgn0016697

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0016697

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG10938-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016697

FlyMine - An integrated database for Drosophila genomics

FBgn0016697

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

36951

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

MIST (protein-protein) - An integrated Molecular Interaction Database

Prosalpha5

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (20)

Reported As

Symbol Synonym

154644_at

(Kristensen et al., 2005)

Alpha-5

(Lundgren et al., 2005)

CG10938

(Çiçek et al., 2016, Hosono et al., 2015, Wang et al., 2014, Schertel et al., 2013, Yoshigi et al., 2013, Yano et al., 2012, Cammarato et al., 2011, Ni et al., 2011.7.19, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Pan and Zhang, 2009, Chen et al., 2008, Barbash and Lorigan, 2007, Lundgren et al., 2005, Ma et al., 2002)

PRCZ

(Mushegian et al., 1998)

PSA5_DROME

(Storm and Sonnhammer, 2003)

PSMA5

(Ma et al., 2002, Zaiss and Belote, 1997, Zaiss, 1996.7.22)

ProsMA5

(Schertel et al., 2013, Chen et al., 2008, Hoopfer et al., 2008, Zhou et al., 2008, Barbash and Lorigan, 2007, Lundgren et al., 2005)

Prosalpha5

(Zhang and Zhang, 2019, Sap et al., 2017, McPhee et al., 2013)

Prosα5

(Johnson et al., 2019, Zhou et al., 2019, Yuan et al., 2015, Lőw et al., 2013, Velentzas et al., 2013, Yoshigi et al., 2013, Ma et al., 2002)

alpha5

(Velentzas et al., 2013, Yano et al., 2012)

prosMA5

(Jasper et al., 2002)

α-5

(Wang et al., 2014)

α5

(Velentzas et al., 2020, Cho-Park and Steller, 2013, Velentzas et al., 2013, Belote and Zhong, 2009)

α5_dm

(Zaiss and Belote, 1997)

Name Synonyms

20S proteasome

(Bohm et al., 2000)

20s proteasome

(Speese et al., 2003)

Proteasome alpha subunit

(Cammarato et al., 2011)

Proteasome α subunit

Proteasome α5 subunit

α-subunit of 20S proteasome

(Fredriksson et al., 2012)

α5

(Nickell et al., 2009)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (82)

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