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Gene Dmel\Prosα5

General Information
Symbol	Dmel\Prosα5	Species	D. melanogaster
Name	Proteasome α5 subunit	Annotation symbol	CG10938
Feature type	protein_coding_gene	FlyBase ID	FBgn0016697
Gene Model Status	Current	Stock availability	7 publicly available
Also Known As	prosMA5, PSMA5
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	54B7-54B7	Sequence location	2R:13,300,272..13,301,274 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Proteasome α5 subunit is referred to in FlyBase by the symbol Dmel\Prosα5 (CG10938, FBgn0016697). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: endopeptidase activity. There is experimental evidence that it is involved in the biological process: cellular process. 6 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Proteasome A-type subunit; Proteasome, alpha-subunit, N-terminal domain; Proteasome, subunit alpha/beta. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, adult Malpighian tubules. Comments on Affy2 ProbeSet: ProbeSet 1641432_a_at completely aligns to an exonic region common to each of the 2 FlyBase-annotated transcript isoforms of Prosalpha5. Gene sequence location is 2R:13300272..13301274.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 54B7-54B7   Limits computationally determined from genome sequence between P{EP}EP1061&P{EP}cnkEP576 and P{EP}l(2)k01209EP2177&P{lacW}k11012a
Experimentally Determined Cytological Location
Cytogenetic map Notes References 54B-54B     (Zaiss, 1996.7.22) 54B3-54B5   (determined by in situ hybridisation)   (Zaiss and Belote, 1997)
Experimentally Determined Recombination Data
Location	2- (Zaiss, 1996.7.22, )
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Prosα5 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Prosα5-RA FBtr0086910  NM_166221   884   244 Prosα5-RB FBtr0086911  NM_057854   873   244
Additional Transcript Data & Comments
Reported size (kB)	0.9 (longest cDNA) (Zaiss and Belote, 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Prosα5-PA   FBpp0086066   26.9   244   4.63     NP_725669   AAF57875 Prosα5-PB   FBpp0086067   26.9   244   4.63     NP_477202   AAM70874
Additional Polypeptide Data & Comments
Reported size (kDa)	244 (aa); 27 (kD predicted) (Zaiss and Belote, 1997)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Proteasome, alpha-subunit, N-terminal domain (IPR000426) Proteasome, subunit alpha/beta (IPR001353) Proteasome A-type subunit (IPR023332) MEROPS - An information resource for peptidases and the proteins that inhibit them • T01.975
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201364   AA201952   AA264479   AA390425   AA391274   AA440308   AA696145   AA735695   AA803318   AA821098   AA979060   AE013599 AAF57875   AE013599 AAM70874   AI260673   AI296051   AI296400   AI514973   AI516382   AI518517   AI546189   AW943176   AY061404 AAL28952   AY232148   BE976219   BF485559   BF491075   BF491736   BF497630   BF503998   BI166959   BI168759   BI170729   BI214434   BI229772   BI229821   BI230516   BI230552   BI231936   BI236910   BI243981   BI351810   BI353491   BI357898   BI363005   BI366953   BI367028   BI370454   BI373551   BI563985   BI565251   BI569250   BI569251   BI569735   BI569968   BI571291   BI571692   BI586870   BI615024   BI620980   BI623404   CK605189   CK605190   CK658625   CK662173   CO155687   CO298358   CO298509   CO322858   CO325373   CZ470030   CZ470035   EC086397   EC222646   EC246449   EC262047   EL881475   EL881739   EV586605   EV598519   GH739344   GH739927   GH741615   GH743904   GH746214   GH763198   GH763377   GH765481   GH765798   GH766464   GH768977   GH769287   GH769785   U64721 AAB93421
UniProtKB/Swiss-Prot	Q95083
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) embryonic stage 7 -- 8   anterior endoderm anlage   (Fisher et al., 2012) head mesoderm   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) pole cell   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   germline cell   (Fisher et al., 2012) embryonic stage 11 -- 12   germline cell   (Fisher et al., 2012) embryonic stage 13 -- 16   germline cell   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
proteasome core complex inferred from direct assay (Sumegi et al., 2003)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult central nervous system, adult Malpighian tubules. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1108.1 Larval Midgut    1325.4 Larval Hindgut    1327.2 Larval Malpighian Tubules    2136.1 Larval Fat Body    1091 Larval Salivary Gland    1459.3 Larval Trachea    1208.85 Larval Carcass    2094.925 Adult Head    590.5 Adult Eye    582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop    898.4 Adult Midgut    796.3 Adult Hindgut    707.3 Adult Malpighian Tubules    438.2 Adult Fat Body    1835 Adult Salivary Gland    518.2 Adult Heart    2107.15 Adult VirginFemale Spermatheca    522.1 Adult InseminatedFemale Spermatheca    561.2 Adult Ovary    1225.3 Adult Testis    875.3 Adult Male Accessory Gland    909.8 Adult Carcass    1388.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1108.1) Larval Midgut  (1325.4) Larval Hindgut  (1327.2) Larval Malpighian Tubules  (2136.1) Larval Fat Body  (1091) Larval Salivary Gland  (1459.3) Larval Trachea  (1208.85) Larval Carcass  (2094.925) Adult Head  (590.5) Adult Eye  (582.625) Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop  (898.4) Adult Midgut  (796.3) Adult Hindgut  (707.3) Adult Malpighian Tubules    438.2 Adult Fat Body  (1835) Adult Salivary Gland  518.2 Adult Heart  (2107.15) Adult VirginFemale Spermatheca  522.1 Adult InseminatedFemale Spermatheca  (561.2) Adult Ovary  (1225.3) Adult Testis  (875.3) Adult Male Accessory Gland  (909.8) Adult Carcass  (1388.9) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1108.1) Larval Midgut  (1325.4) Larval Hindgut  (1327.2) Larval Malpighian Tubules  (2136.1) Larval Fat Body  (1091) Larval Salivary Gland  (1459.3) Larval Trachea  (1208.85) Larval Carcass  (2094.925) Adult Head    590.5 Adult Eye    582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop    898.4 Adult Midgut    796.3 Adult Hindgut    707.3 Adult Malpighian Tubules    438.2 Adult Fat Body  (1835) Adult Salivary Gland    518.2 Adult Heart  (2107.15) Adult VirginFemale Spermatheca    522.1 Adult InseminatedFemale Spermatheca    561.2 Adult Ovary  (1225.3) Adult Testis    875.3 Adult Male Accessory Gland    909.8 Adult Carcass  (1388.9) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1108.1 Larval Midgut    1325.4 Larval Hindgut    1327.2 Larval Malpighian Tubules    2136.1 Larval Fat Body    1091 Larval Salivary Gland    1459.3 Larval Trachea    1208.85 Larval Carcass    2094.925 Adult Head    590.5 Adult Eye    582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop    898.4 Adult Midgut    796.3 Adult Hindgut    707.3 Adult Malpighian Tubules    438.2 Adult Fat Body    1835 Adult Salivary Gland    518.2 Adult Heart    2107.15 Adult VirginFemale Spermatheca    522.1 Adult InseminatedFemale Spermatheca    561.2 Adult Ovary    1225.3 Adult Testis    875.3 Adult Male Accessory Gland    909.8 Adult Carcass    1388.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1108.1 Larval Midgut    1325.4 Larval Hindgut    1327.2 Larval Malpighian Tubules    2136.1 Larval Fat Body    1091 Larval Salivary Gland    1459.3 Larval Trachea    1208.85 Larval Carcass    2094.925 Adult Head    590.5 Adult Eye    582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop    898.4 Adult Midgut    796.3 Adult Hindgut    707.3 Adult Malpighian Tubules    438.2 Adult Fat Body    1835 Adult Salivary Gland    518.2 Adult Heart    2107.15 Adult VirginFemale Spermatheca    522.1 Adult InseminatedFemale Spermatheca    561.2 Adult Ovary    1225.3 Adult Testis    875.3 Adult Male Accessory Gland    909.8 Adult Carcass    1388.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1108.1) Larval Midgut  (1325.4) Larval Hindgut  (1327.2) Larval Malpighian Tubules  (2136.1) Larval Fat Body  (1091) Larval Salivary Gland  (1459.3) Larval Trachea  (1208.85) Larval Carcass  (2094.925) Adult Head  590.5 Adult Eye  582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop  (898.4) Adult Midgut  796.3 Adult Hindgut  707.3 Adult Malpighian Tubules    438.2 Adult Fat Body  (1835) Adult Salivary Gland  518.2 Adult Heart  (2107.15) Adult VirginFemale Spermatheca  522.1 Adult InseminatedFemale Spermatheca  561.2 Adult Ovary  (1225.3) Adult Testis  (875.3) Adult Male Accessory Gland  (909.8) Adult Carcass  (1388.9) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1108.1 Larval Midgut  1325.4 Larval Hindgut  1327.2 Larval Malpighian Tubules  (2136.1) Larval Fat Body  1091 Larval Salivary Gland  1459.3 Larval Trachea  1208.85 Larval Carcass  (2094.925) Adult Head    590.5 Adult Eye    582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop    898.4 Adult Midgut    796.3 Adult Hindgut    707.3 Adult Malpighian Tubules    438.2 Adult Fat Body  (1835) Adult Salivary Gland    518.2 Adult Heart  (2107.15) Adult VirginFemale Spermatheca    522.1 Adult InseminatedFemale Spermatheca    561.2 Adult Ovary  1225.3 Adult Testis    875.3 Adult Male Accessory Gland    909.8 Adult Carcass  1388.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1108.1 Larval Midgut    1325.4 Larval Hindgut    1327.2 Larval Malpighian Tubules    2136.1 Larval Fat Body    1091 Larval Salivary Gland    1459.3 Larval Trachea    1208.85 Larval Carcass    2094.925 Adult Head    590.5 Adult Eye    582.625 Adult Brain    350.8 Adult Thoracic-Abdominal Ganglion    400 Adult Crop    898.4 Adult Midgut    796.3 Adult Hindgut    707.3 Adult Malpighian Tubules    438.2 Adult Fat Body    1835 Adult Salivary Gland    518.2 Adult Heart    2107.15 Adult VirginFemale Spermatheca    522.1 Adult InseminatedFemale Spermatheca    561.2 Adult Ovary    1225.3 Adult Testis    875.3 Adult Male Accessory Gland    909.8 Adult Carcass    1388.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    590.5   NA  Eye    582.625   NA  Brain    350.8   1108.1  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    400   NA  Crop    898.4   1325.4  Midgut    796.3   1327.2  Hindgut    707.3   2136.1  Malpighian Tubules    438.2   1091  Fat Body    1835   1459.3  Salivary Gland    518.2   NA  Heart    2107.15   1208.85  Trachea    NA   NA  VirginFemale Spermatheca    522.1   NA  InseminatedFemale Spermatheca    561.2   NA  Ovary    1225.3   NA  Testis    875.3   NA  Male Accessory Gland    909.8   2094.925  Carcass    1388.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    39 central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male    31 salivary gland, larvae L3 wandering    29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering    76 digestive system, 1-day adult    40 digestive system, 4-day adult    32 digestive system, 20-day adult    26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering    96 carcass, 1-day adult    25 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    77 ovary, mated 4-day female    67 testis, mated 4-day male    110 accessory gland, mated 4-day male    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (61) central nervous system, larvae L3  (39) central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male  (31) salivary gland, larvae L3 wandering  (29) salivary gland, white prepupae    17 digestive system, larvae L3 wandering  (76) digestive system, 1-day adult  (40) digestive system, 4-day adult  (32) digestive system, 20-day adult  26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering  (96) carcass, 1-day adult    25 carcass, 4-day adult  (27) carcass, 20-day adult    23 ovary, virgin 4-day female  (77) ovary, mated 4-day female  (67) testis, mated 4-day male  (110) accessory gland, mated 4-day male  (30) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    39 central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male    31 salivary gland, larvae L3 wandering    29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering    76 digestive system, 1-day adult    40 digestive system, 4-day adult    32 digestive system, 20-day adult    26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering    96 carcass, 1-day adult    25 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    77 ovary, mated 4-day female    67 testis, mated 4-day male  (110) accessory gland, mated 4-day male    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    39 central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male    31 salivary gland, larvae L3 wandering    29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering    76 digestive system, 1-day adult    40 digestive system, 4-day adult    32 digestive system, 20-day adult    26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering    96 carcass, 1-day adult    25 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    77 ovary, mated 4-day female    67 testis, mated 4-day male    110 accessory gland, mated 4-day male    30 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    39 central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male    31 salivary gland, larvae L3 wandering    29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering    76 digestive system, 1-day adult    40 digestive system, 4-day adult    32 digestive system, 20-day adult    26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering    96 carcass, 1-day adult    25 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    77 ovary, mated 4-day female    67 testis, mated 4-day male    110 accessory gland, mated 4-day male    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (61) central nervous system, larvae L3  (39) central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male  31 salivary gland, larvae L3 wandering  29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering  (76) digestive system, 1-day adult  (40) digestive system, 4-day adult  32 digestive system, 20-day adult  26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering  (96) carcass, 1-day adult    25 carcass, 4-day adult  27 carcass, 20-day adult    23 ovary, virgin 4-day female  (77) ovary, mated 4-day female  (67) testis, mated 4-day male  (110) accessory gland, mated 4-day male  30 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    39 central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male    31 salivary gland, larvae L3 wandering    29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering    76 digestive system, 1-day adult    40 digestive system, 4-day adult    32 digestive system, 20-day adult    26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering    96 carcass, 1-day adult    25 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    77 ovary, mated 4-day female    67 testis, mated 4-day male  110 accessory gland, mated 4-day male    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    61 central nervous system, larvae L3    39 central nervous system, pupae P8    16 head, virgin 1-day female    16 head, virgin 4-day female    10 head, virgin 20-day female    15 head, mated 1-day female    8 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    15 head, mated 4-day male    19 head, mated 20-day male    31 salivary gland, larvae L3 wandering    29 salivary gland, white prepupae    17 digestive system, larvae L3 wandering    76 digestive system, 1-day adult    40 digestive system, 4-day adult    32 digestive system, 20-day adult    26 fat body, larvae L3 wandering    10 fat body, white prepupae    25 fat body, pupae P8    11 carcass, larvae L3 wandering    96 carcass, 1-day adult    25 carcass, 4-day adult    27 carcass, 20-day adult    23 ovary, virgin 4-day female    77 ovary, mated 4-day female    67 testis, mated 4-day male    110 accessory gland, mated 4-day male    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-06 hour embryonic stages, during late larval stages, at stages throughout the pupal period, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    350 embryo 02-04hr    409 embryo 04-06hr    135 embryo 06-08hr    92 embryo 08-10hr    114 embryo 10-12hr    111 embryo 12-14hr    62 embryo 14-16hr    61 embryo 16-18hr    39 embryo 18-20hr    29 embryo 20-22hr    33 embryo 22-24hr    29 larva L1    57 larva L2    127 larva L3 12hr old    112 larva L3 puffstage 1-2    154 larva L3 puffstage 3-6    267 larva L3 puffstage 7-9    329 white prepupae new    345 white prepupae 12hr    147 white prepupae 24hr    154 pupae 2d postWPP    110 pupae 3d postWPP    85 pupae 4d postWPP    155 adult male 01day    129 adult male 05day    137 adult male 30day    141 adult female 01day    173 adult female 05day    207 adult female 30day    203 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (350) embryo 02-04hr  (409) embryo 04-06hr  (135) embryo 06-08hr  (92) embryo 08-10hr  (114) embryo 10-12hr  (111) embryo 12-14hr  (62) embryo 14-16hr  (61) embryo 16-18hr  (39) embryo 18-20hr  (29) embryo 20-22hr  (33) embryo 22-24hr  (29) larva L1  (57) larva L2  (127) larva L3 12hr old  (112) larva L3 puffstage 1-2  (154) larva L3 puffstage 3-6  (267) larva L3 puffstage 7-9  (329) white prepupae new  (345) white prepupae 12hr  (147) white prepupae 24hr  (154) pupae 2d postWPP  (110) pupae 3d postWPP  (85) pupae 4d postWPP  (155) adult male 01day  (129) adult male 05day  (137) adult male 30day  (141) adult female 01day  (173) adult female 05day  (207) adult female 30day  (203) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (350) embryo 02-04hr  (409) embryo 04-06hr  (135) embryo 06-08hr    92 embryo 08-10hr  (114) embryo 10-12hr  (111) embryo 12-14hr    62 embryo 14-16hr    61 embryo 16-18hr    39 embryo 18-20hr    29 embryo 20-22hr    33 embryo 22-24hr    29 larva L1    57 larva L2  (127) larva L3 12hr old  (112) larva L3 puffstage 1-2  (154) larva L3 puffstage 3-6  (267) larva L3 puffstage 7-9  (329) white prepupae new  (345) white prepupae 12hr  (147) white prepupae 24hr  (154) pupae 2d postWPP  (110) pupae 3d postWPP    85 pupae 4d postWPP  (155) adult male 01day  (129) adult male 05day  (137) adult male 30day  (141) adult female 01day  (173) adult female 05day  (207) adult female 30day  (203) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    350 embryo 02-04hr    409 embryo 04-06hr    135 embryo 06-08hr    92 embryo 08-10hr    114 embryo 10-12hr    111 embryo 12-14hr    62 embryo 14-16hr    61 embryo 16-18hr    39 embryo 18-20hr    29 embryo 20-22hr    33 embryo 22-24hr    29 larva L1    57 larva L2    127 larva L3 12hr old    112 larva L3 puffstage 1-2    154 larva L3 puffstage 3-6    267 larva L3 puffstage 7-9    329 white prepupae new    345 white prepupae 12hr    147 white prepupae 24hr    154 pupae 2d postWPP    110 pupae 3d postWPP    85 pupae 4d postWPP    155 adult male 01day    129 adult male 05day    137 adult male 30day    141 adult female 01day    173 adult female 05day    207 adult female 30day    203 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    350 embryo 02-04hr    409 embryo 04-06hr    135 embryo 06-08hr    92 embryo 08-10hr    114 embryo 10-12hr    111 embryo 12-14hr    62 embryo 14-16hr    61 embryo 16-18hr    39 embryo 18-20hr    29 embryo 20-22hr    33 embryo 22-24hr    29 larva L1    57 larva L2    127 larva L3 12hr old    112 larva L3 puffstage 1-2    154 larva L3 puffstage 3-6    267 larva L3 puffstage 7-9    329 white prepupae new    345 white prepupae 12hr    147 white prepupae 24hr    154 pupae 2d postWPP    110 pupae 3d postWPP    85 pupae 4d postWPP    155 adult male 01day    129 adult male 05day    137 adult male 30day    141 adult female 01day    173 adult female 05day    207 adult female 30day    203 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (350) embryo 02-04hr  (409) embryo 04-06hr  (135) embryo 06-08hr  (92) embryo 08-10hr  (114) embryo 10-12hr  (111) embryo 12-14hr  (62) embryo 14-16hr  (61) embryo 16-18hr  (39) embryo 18-20hr  29 embryo 20-22hr  (33) embryo 22-24hr  29 larva L1  (57) larva L2  (127) larva L3 12hr old  (112) larva L3 puffstage 1-2  (154) larva L3 puffstage 3-6  (267) larva L3 puffstage 7-9  (329) white prepupae new  (345) white prepupae 12hr  (147) white prepupae 24hr  (154) pupae 2d postWPP  (110) pupae 3d postWPP  (85) pupae 4d postWPP  (155) adult male 01day  (129) adult male 05day  (137) adult male 30day  (141) adult female 01day  (173) adult female 05day  (207) adult female 30day  (203) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (350) embryo 02-04hr  (409) embryo 04-06hr  135 embryo 06-08hr    92 embryo 08-10hr  114 embryo 10-12hr  111 embryo 12-14hr    62 embryo 14-16hr    61 embryo 16-18hr    39 embryo 18-20hr    29 embryo 20-22hr    33 embryo 22-24hr    29 larva L1    57 larva L2  127 larva L3 12hr old  112 larva L3 puffstage 1-2  (154) larva L3 puffstage 3-6  (267) larva L3 puffstage 7-9  (329) white prepupae new  (345) white prepupae 12hr  (147) white prepupae 24hr  (154) pupae 2d postWPP  110 pupae 3d postWPP    85 pupae 4d postWPP  (155) adult male 01day  129 adult male 05day  137 adult male 30day  141 adult female 01day  (173) adult female 05day  (207) adult female 30day  (203) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    350 embryo 02-04hr    409 embryo 04-06hr    135 embryo 06-08hr    92 embryo 08-10hr    114 embryo 10-12hr    111 embryo 12-14hr    62 embryo 14-16hr    61 embryo 16-18hr    39 embryo 18-20hr    29 embryo 20-22hr    33 embryo 22-24hr    29 larva L1    57 larva L2    127 larva L3 12hr old    112 larva L3 puffstage 1-2    154 larva L3 puffstage 3-6    267 larva L3 puffstage 7-9    329 white prepupae new    345 white prepupae 12hr    147 white prepupae 24hr    154 pupae 2d postWPP    110 pupae 3d postWPP    85 pupae 4d postWPP    155 adult male 01day    129 adult male 05day    137 adult male 30day    141 adult female 01day    173 adult female 05day    207 adult female 30day    203 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    135 Schneider line 2 Sg4    254 embryonic 1182-4H    39 embryonic GM2    79 embryonic Kc167    123 embryonic S1    86 embryonic S3    127 leg disc CME L1    94 wing disc CME-W2    126 wing disc ML-DmD8    99 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    136 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    99 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    144 mixed discs ML-DmD4-c1    113 CNS ML-DmBG1-c1    67 CNS ML-DmBG2-c2    306 tumorous blood cells mbn2    84 ovary fGS/OSS    27 ovary OSC    36 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (135) Schneider line 2 Sg4  (254) embryonic 1182-4H  (39) embryonic GM2  (79) embryonic Kc167  (123) embryonic S1  (86) embryonic S3  (127) leg disc CME L1  (94) wing disc CME-W2  (126) wing disc ML-DmD8  (99) wing disc ML-DmD9  (48) wing disc ML-DmD16-c3  (63) wing disc ML-DmD21  (136) wing disc ML-DmD32  (34) haltere disc ML-DmD17-c3  (99) eye-antennal disc ML-DmD11  (45) antennal disc ML-DmD20-c5  (144) mixed discs ML-DmD4-c1  (113) CNS ML-DmBG1-c1  (67) CNS ML-DmBG2-c2  (306) tumorous blood cells mbn2  (84) ovary fGS/OSS  (27) ovary OSC  (36) ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (135) Schneider line 2 Sg4  (254) embryonic 1182-4H    39 embryonic GM2    79 embryonic Kc167  (123) embryonic S1    86 embryonic S3  (127) leg disc CME L1    94 wing disc CME-W2  (126) wing disc ML-DmD8    99 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    63 wing disc ML-DmD21  (136) wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    99 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5  (144) mixed discs ML-DmD4-c1  (113) CNS ML-DmBG1-c1    67 CNS ML-DmBG2-c2  (306) tumorous blood cells mbn2    84 ovary fGS/OSS    27 ovary OSC    36 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    135 Schneider line 2 Sg4    254 embryonic 1182-4H    39 embryonic GM2    79 embryonic Kc167    123 embryonic S1    86 embryonic S3    127 leg disc CME L1    94 wing disc CME-W2    126 wing disc ML-DmD8    99 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    136 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    99 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    144 mixed discs ML-DmD4-c1    113 CNS ML-DmBG1-c1    67 CNS ML-DmBG2-c2    306 tumorous blood cells mbn2    84 ovary fGS/OSS    27 ovary OSC    36 ovary OSS    21 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    135 Schneider line 2 Sg4    254 embryonic 1182-4H    39 embryonic GM2    79 embryonic Kc167    123 embryonic S1    86 embryonic S3    127 leg disc CME L1    94 wing disc CME-W2    126 wing disc ML-DmD8    99 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    136 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    99 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    144 mixed discs ML-DmD4-c1    113 CNS ML-DmBG1-c1    67 CNS ML-DmBG2-c2    306 tumorous blood cells mbn2    84 ovary fGS/OSS    27 ovary OSC    36 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (135) Schneider line 2 Sg4  (254) embryonic 1182-4H  (39) embryonic GM2  (79) embryonic Kc167  (123) embryonic S1  (86) embryonic S3  (127) leg disc CME L1  (94) wing disc CME-W2  (126) wing disc ML-DmD8  (99) wing disc ML-DmD9  (48) wing disc ML-DmD16-c3  (63) wing disc ML-DmD21  (136) wing disc ML-DmD32  (34) haltere disc ML-DmD17-c3  (99) eye-antennal disc ML-DmD11  (45) antennal disc ML-DmD20-c5  (144) mixed discs ML-DmD4-c1  (113) CNS ML-DmBG1-c1  (67) CNS ML-DmBG2-c2  (306) tumorous blood cells mbn2  (84) ovary fGS/OSS  27 ovary OSC  (36) ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  135 Schneider line 2 Sg4  (254) embryonic 1182-4H    39 embryonic GM2    79 embryonic Kc167  123 embryonic S1    86 embryonic S3  127 leg disc CME L1    94 wing disc CME-W2  126 wing disc ML-DmD8    99 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    63 wing disc ML-DmD21  136 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    99 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5  (144) mixed discs ML-DmD4-c1  113 CNS ML-DmBG1-c1    67 CNS ML-DmBG2-c2  (306) tumorous blood cells mbn2    84 ovary fGS/OSS    27 ovary OSC    36 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    135 Schneider line 2 Sg4    254 embryonic 1182-4H    39 embryonic GM2    79 embryonic Kc167    123 embryonic S1    86 embryonic S3    127 leg disc CME L1    94 wing disc CME-W2    126 wing disc ML-DmD8    99 wing disc ML-DmD9    48 wing disc ML-DmD16-c3    63 wing disc ML-DmD21    136 wing disc ML-DmD32    34 haltere disc ML-DmD17-c3    99 eye-antennal disc ML-DmD11    45 antennal disc ML-DmD20-c5    144 mixed discs ML-DmD4-c1    113 CNS ML-DmBG1-c1    67 CNS ML-DmBG2-c2    306 tumorous blood cells mbn2    84 ovary fGS/OSS    27 ovary OSC    36 ovary OSS    21 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    27 cold shock, 4-day adult    23 heat shock, 4-day adult    72 Cadmium 50 mM 6 hrs, larvae L3    93 Cadmium 50 mM 12 hrs, larvae L3    138 Cadmium 50 mM 48 hrs, 4-day adult    56 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    94 Ethanol 5% 3 hrs, larvae L3    70 Ethanol 10% 3 hrs, larvae L3    73 Caffeine 1.5 mg/ml 4 hrs, larvae L3    66 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    110 Rotenone 2 μg 12 hrs, larvae L3    74 Rotenone 8 μg 12 hrs, larvae L3    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (27) cold shock, 4-day adult    23 heat shock, 4-day adult  (72) Cadmium 50 mM 6 hrs, larvae L3  (93) Cadmium 50 mM 12 hrs, larvae L3  (138) Cadmium 50 mM 48 hrs, 4-day adult  (56) Cadmium 100 mM 48 hrs, 4-day adult  (56) Copper 0.5 mM 12 hrs, larvae L3  (35) Copper 15 mM 48 hrs, 4-day adult  (61) Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult  (30) Ethanol 2.5% 3 hrs, larvae L3  (94) Ethanol 5% 3 hrs, larvae L3  (70) Ethanol 10% 3 hrs, larvae L3  (73) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (66) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (53) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (56) Paraquat 10 mM 48 hrs, 4-day adult  (110) Rotenone 2 μg 12 hrs, larvae L3  (74) Rotenone 8 μg 12 hrs, larvae L3  (69) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    27 cold shock, 4-day adult    23 heat shock, 4-day adult    72 Cadmium 50 mM 6 hrs, larvae L3    93 Cadmium 50 mM 12 hrs, larvae L3  (138) Cadmium 50 mM 48 hrs, 4-day adult    56 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    94 Ethanol 5% 3 hrs, larvae L3    70 Ethanol 10% 3 hrs, larvae L3    73 Caffeine 1.5 mg/ml 4 hrs, larvae L3    66 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult  (110) Rotenone 2 μg 12 hrs, larvae L3    74 Rotenone 8 μg 12 hrs, larvae L3    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    27 cold shock, 4-day adult    23 heat shock, 4-day adult    72 Cadmium 50 mM 6 hrs, larvae L3    93 Cadmium 50 mM 12 hrs, larvae L3    138 Cadmium 50 mM 48 hrs, 4-day adult    56 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    94 Ethanol 5% 3 hrs, larvae L3    70 Ethanol 10% 3 hrs, larvae L3    73 Caffeine 1.5 mg/ml 4 hrs, larvae L3    66 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    110 Rotenone 2 μg 12 hrs, larvae L3    74 Rotenone 8 μg 12 hrs, larvae L3    69 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    27 cold shock, 4-day adult    23 heat shock, 4-day adult    72 Cadmium 50 mM 6 hrs, larvae L3    93 Cadmium 50 mM 12 hrs, larvae L3    138 Cadmium 50 mM 48 hrs, 4-day adult    56 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    94 Ethanol 5% 3 hrs, larvae L3    70 Ethanol 10% 3 hrs, larvae L3    73 Caffeine 1.5 mg/ml 4 hrs, larvae L3    66 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    110 Rotenone 2 μg 12 hrs, larvae L3    74 Rotenone 8 μg 12 hrs, larvae L3    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  27 cold shock, 4-day adult    23 heat shock, 4-day adult  (72) Cadmium 50 mM 6 hrs, larvae L3  (93) Cadmium 50 mM 12 hrs, larvae L3  (138) Cadmium 50 mM 48 hrs, 4-day adult  (56) Cadmium 100 mM 48 hrs, 4-day adult  (56) Copper 0.5 mM 12 hrs, larvae L3  (35) Copper 15 mM 48 hrs, 4-day adult  (61) Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult  30 Ethanol 2.5% 3 hrs, larvae L3  (94) Ethanol 5% 3 hrs, larvae L3  (70) Ethanol 10% 3 hrs, larvae L3  (73) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (66) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (53) Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult  (56) Paraquat 10 mM 48 hrs, 4-day adult  (110) Rotenone 2 μg 12 hrs, larvae L3  (74) Rotenone 8 μg 12 hrs, larvae L3  (69) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    27 cold shock, 4-day adult    23 heat shock, 4-day adult    72 Cadmium 50 mM 6 hrs, larvae L3    93 Cadmium 50 mM 12 hrs, larvae L3  138 Cadmium 50 mM 48 hrs, 4-day adult    56 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    94 Ethanol 5% 3 hrs, larvae L3    70 Ethanol 10% 3 hrs, larvae L3    73 Caffeine 1.5 mg/ml 4 hrs, larvae L3    66 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult  110 Rotenone 2 μg 12 hrs, larvae L3    74 Rotenone 8 μg 12 hrs, larvae L3    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    27 cold shock, 4-day adult    23 heat shock, 4-day adult    72 Cadmium 50 mM 6 hrs, larvae L3    93 Cadmium 50 mM 12 hrs, larvae L3    138 Cadmium 50 mM 48 hrs, 4-day adult    56 Cadmium 100 mM 48 hrs, 4-day adult    56 Copper 0.5 mM 12 hrs, larvae L3    35 Copper 15 mM 48 hrs, 4-day adult    61 Zinc 5 mM 12 hrs, larvae L3    22 Zinc 4.5 mM 48 hrs, 4-day adult    30 Ethanol 2.5% 3 hrs, larvae L3    94 Ethanol 5% 3 hrs, larvae L3    70 Ethanol 10% 3 hrs, larvae L3    73 Caffeine 1.5 mg/ml 4 hrs, larvae L3    66 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    22 Paraquat 5 mM 48 hrs, 4-day adult    56 Paraquat 10 mM 48 hrs, 4-day adult    110 Rotenone 2 μg 12 hrs, larvae L3    74 Rotenone 8 μg 12 hrs, larvae L3    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_02 mE1_20_mRNA_expression_cluster_10
External Data & Images
Linkouts	BDGP expression data • LD33318 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016697 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10938-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Prosα5 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 Prosα5GD7134 Phenotype manifest in Allele
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Prosα5 Class Mutagen Stocks Known lesion Prosα5c05490 piggyBac transposase 1 -- Prosα5c05495 piggyBac transposase 1 -- Prosα5NP3047 1 --
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Prosα5 Class Mutagen Stocks Known lesion Prosα5GD7134 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Prosα5HMS00095 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Prosα5KK108214 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel7147 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{GT1}BG02176 NA PBac{PB}Prosα5c05490 NA PBac{PB}Prosα5c05495 NA insertion of enhancer trap binary system P{GawB}Prosα5NP3047 No
Gene Ontology: Function, Process & Cellular Component ( 9 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
cellular process inferred from mutant phenotype (Boutros et al., 2004)
Cellular Component
CV term Evidence References
proteasome core complex inferred from direct assay (Sumegi et al., 2003)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
endopeptidase activity non-traceable author statement (Zaiss, 1996.7.22, FlyBase, 1992-, Swiss-Prot Project Members, 1997.2.1) contributes_to endopeptidase activity inferred by curator from GO:0005839 (Sumegi et al., 2003) threonine-type endopeptidase activity inferred from electronic annotation with InterPro:IPR001353, InterPro:IPR023332 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
proteasomal ubiquitin-dependent protein catabolic process inferred by curator from GO:0005839 (Sumegi et al., 2003) proteolysis non-traceable author statement (Swiss-Prot Project Members, 1997.2.1) ubiquitin-dependent protein catabolic process inferred from electronic annotation with InterPro:IPR000426 (FlyBase Curators et al., 2004-) non-traceable author statement (Swiss-Prot Project Members, 1997.2.1)
Cellular Component
CV term Evidence References
proteasome complex non-traceable author statement (Zaiss, 1996.7.22, Swiss-Prot Project Members, 1997.2.1) proteasome core complex non-traceable author statement (FlyBase, 1992-) proteasome core complex, alpha-subunit complex inferred from electronic annotation with InterPro:IPR000426 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Prosα5 - 4EHP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - AnxB10 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Aos1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Bx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG10082 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG11060 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG11191 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG11885 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - CG12321 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - CG13319 experimental knowledge based (Guruharsha et al., 2011) Prosα5 - CG13321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG15011 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG15601 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG1598 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG17834 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG18598 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG2046 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - CG2076 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG2812 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG2931 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG31200 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG31802 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG31917 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG32485 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG33213 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG34133 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG3795 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG3887 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG43658 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG4407 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG4766 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG4781 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG5167 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG5196 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG5347 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG5660 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG6321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG6337 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG6852 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG7272 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG8195 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG8314 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG8368 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG8509 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG8525 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - CG8630 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Cht2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Cp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Cyp4e3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Cyp4g15 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Eno anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - GNBP2 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Gadd34 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - GstE11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Ime4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - IntS10 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Klc experimental knowledge based (Guruharsha et al., 2011) Prosα5 - MFS16 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Naam anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - NimB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - O-fut1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Patsas anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Pomp anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosα1 experimental knowledge based (Guruharsha et al., 2011) Prosα5 - Prosα2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosα3 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosα4 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosα6 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Prosα6T anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Prosα7 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Prosβ1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosβ2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosβ2R1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Prosβ2R2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Prosβ3 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosβ4 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosβ4R1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Prosβ4R2 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Prosβ5 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosβ6 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prosβ7 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Prp31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - REG experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rab18 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - RpIII128 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Rpn1 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Rpn11 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpn13 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpn2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Rpn6 experimental knowledge based (Guruharsha et al., 2011) Prosα5 - Rpn7 experimental knowledge based (Guruharsha et al., 2011) Prosα5 - Rpn8 experimental knowledge based (Guruharsha et al., 2011) Prosα5 - Rpn9 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpt1 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpt2 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Rpt3 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpt4 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpt5 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Prosα5 - Rpt6 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Prosα5 - Smn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Spf45 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Tapδ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Tgt anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Tim9a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Uch-L5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - Vha100-4 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - aur anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - boly anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - cold anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - crn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - emb anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - fbp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - mRpL48 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - mRpL50 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - mip130 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - nes anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - p53 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - rpr anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - shf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - stet anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Prosα5 - εCOP anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Prosα5 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62645 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0016697 DroID - A comprehensive database of gene and protein interactions. • FBgn0016697 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36951
Orthologs
OrthoDB Orthologs (39) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6V17M2 Dipteran inclusive ortholog search EOG6CJVS3 Insect inclusive ortholog search EOG6X96D6 Arthropod inclusive ortholog search EOG6Z08NN Metazoa inclusive ortholog search EOG6DRP6J
Orthologs in Drosophila Species (EOG6V17M2)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Prosα5     Drosophila simulans   Dsim\GD11263 Y   Drosophila sechellia   Dsec\GM21770 Y   Drosophila erecta   Dere\GG20675 Y   Drosophila yakuba   Dyak\ProsMA5 Y   Drosophila ananassae   Dana\GF13117 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10654 Y   Drosophila persimilis   Dper\GL11421 Y   Drosophila willistoni   Dwil\GK21413 Y   Drosophila virilis   Dvir\GJ20367 Y   Drosophila mojavensis   Dmoj\GI18573 Y   Drosophila grimshawi   Dgri\GH21393 Y
Orthologs in non-Drosophila Dipterans (EOG6CJVS3)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004308   Anopheles gambiae Malaria mosquito  Agam\AGAP008816   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ801754
Orthologs in non-Dipteran Insects (EOG6X96D6)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Prosalpha5   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005999   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH10998   Atta cephalotes Leafcutter ant  Acep\ACEP20044   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14987   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22906   Linepithema humile Argentine ant  Lhum\LH19065   Pogonomyrmex barbatus Red harvester ant  Pbar\PB25969   Solenopsis invicta Red fire ant  Sinv\SINV20825   Acyrthosiphon pisum Pea aphid  Apim\ACYPI009032   Bombyx mori Silkmoth  Bmor\BGIBMGA013898   Pediculus humanus Human body louse  Phum\PHUM116710   Tribolium castaneum Red flour beetle  Tcas\TC008671
Orthologs in non-Insect Arthropods (EOG6Z08NN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\MCM5
Orthologs in non-Arthropod Metazoa (EOG6DRP6J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\pas-5   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_002299gn   Danio rerio Zebrafish  Drer\psma5   Xenopus tropicalis Western clawed frog  Xtro\psma5   Gallus gallus Domestic chicken  Ggal\ENSGALG00000000065   Mus musculus House mouse  Mmus\Gm8394   Mus musculus House mouse  Mmus\Psma5   Rattus norvegicus Norway rat  Rnor\ENSRNOG00000042279   Rattus norvegicus Norway rat  Rnor\Psma5   Homo sapiens Human  Hsap\PSMA5
Human Orthologs (1)
Gene OMIM HGNC Hsap\PSMA5
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11263 Drosophila sechellia Dsec\GM21770 Drosophila erecta Dere\GG20675 Drosophila yakuba Dyak\ProsMA5 Drosophila ananassae Dana\GF13117 Drosophila pseudoobscura pseudoobscura Dpse\GA10654 Drosophila persimilis Dper\GL11421 Drosophila willistoni Dwil\GK21413 Drosophila virilis Dvir\GJ20367 Drosophila mojavensis Dmoj\GI18573 Drosophila grimshawi Dgri\GH21393
Stocks & Reagents
Stocks Listed in FlyBase ( 7 )
Bloomington	34786 y1 sc* v1; P{TRiP.HMS00095}attP2
Harvard	- PBac{PB}Prosα5c05490 - PBac{PB}Prosα5c05495
Kyoto	104356 y* w*; P{GawB}Prosα5NP3047 / CyO, P{UAS-lacZ.UW14}UW14
VDRC	v108380 P{KK108214}VIE-260B v16105 w1118; P{GD7134}v16105 v16104 w1118; P{GD7134}v16104/TM3
Genomic Clones ( 2 )
	BACR21A09 BACR28O12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 85 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD33318
Other clones	DPiM_LD33318 yak-ad_ProsMA5
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT05255 AT11842 AT19592 AT27733 AT28547 GM05155 GM09545 GM09694 GM10560 GM16192 GM23862 LD04093 LD04439 LD08136 LD08707 LD10469 LD15237 LD38057 LD42236 LD46295 LD46622 LP04626 LP09846 LP10271 LP15704 LP22187 RE07216 RE09625 RE12281 RE14210 RE14253 RE20243 RE25118 RE27623 RE27682 RE33040 RE41810 RE44519 RE47711 RE52608 RE52695 RE56975 RE60870 RH01874 RH02179 RH06133 RH06964 RH26982 RH34950 RH44540 RH52250 RH55646 RH62975
Other clones	48420 62220 69273 525468 bs48c05 Dmel_neb_011089_0942_2756 Dmel_neb_011851_0199_1043 EK172833 EK173147 EK189063 EK196331 EN06553 IP11253 IP11553 RH01380 RP001003300 RP001006440 RP001023739 RP001024023 RP001032297 RP001032693 RP001040744 RP001041051 RP001041948 RP001042141 RP001050504 RP001052265 RP001055452 UT01742
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0016697 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36951
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms. (FlyBase, 2009-)
Other Comments
	RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells. (Boutros et al., 2004) ProsMA5 has been cloned and sequenced. (Zaiss and Belote, 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057854 NM_166221 RefSeq (Proteins) • NP_477202 NP_725669 DDBJ / EMBL / GenBank • AA979060 AAB93421 AAF57875 AAL28952 AAM70874 AW943176 AY061404 CZ470030 CZ470035 U64721 Entrez Gene - A searchable database of RefSeq genes. • 36951 UniProtKB/Swiss-Prot • Q95083
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Proteasome, alpha-subunit, N-terminal domain (IPR000426) Proteasome, subunit alpha/beta (IPR001353) Proteasome A-type subunit (IPR023332) MEROPS - An information resource for peptidases and the proteins that inhibit them • T01.975 BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD33318
Linkouts
	BioGRID - A database of protein and genetic interactions • 62645 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0016697 DroID - A comprehensive database of gene and protein interactions. • FBgn0016697 DRSC - Results from RNAi screens. • FBgn0016697 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0016697 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10938-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0016697 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 99021 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36951 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36951 modMine - Data generated by the modENCODE project. • FBgn0016697
Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	154644_at (Kristensen et al., 2005) Alpha-5 (Lundgren et al., 2005) alpha5 (Yano et al., 2012) CG10938 (Barbash and Lorigan, 2007, Keleman et al., 2009.8.5, Ma et al., 2002, Chen et al., 2008, Ni et al., 2011.7.19, Cammarato et al., 2011, Lundgren et al., 2005, Belote and Zhong, 2009, Yano et al., 2012) PRCZ (Mushegian et al., 1998) prosMA5 (Jasper et al., 2002) ProsMA5 (Hoopfer et al., 2008, Barbash and Lorigan, 2007, Chen et al., 2008, Zhou et al., 2008, Lundgren et al., 2005) Prosα5 (Ma et al., 2002) PSA5_DROME (Storm and Sonnhammer, 2003) PSMA5 (Zaiss, 1996.7.22, Zaiss and Belote, 1997, Ma et al., 2002) α5 (Belote and Zhong, 2009) α5_dm (Zaiss and Belote, 1997)
Name Synonym	20s proteasome (Speese et al., 2003) 20S proteasome (Bohm et al., 2000) Proteasome alpha subunit (Cammarato et al., 2011) Proteasome α5 subunit   Proteasome α subunit   α5 (Nickell et al., 2009) α-subunit of 20S proteasome (Fredriksson et al., 2012)
Secondary FlyBase IDs
References ( 47 )
Generate a list of	All references relating to this gene ( 47 )
List References by type	Research paper  ( 21 ) Supplementary material  ( 7 ) Review  ( 1 ) Personal communication to FlyBase  ( 9 ) FlyBase analysis  ( 4 ) DNA/RNA sequence record  ( 1 ) Protein sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 4 )
	Velentzas et al., 2013, Cell Biol. Toxicol. 29(1): 13--37 Detrimental effects of proteasome inhibition activity in Drosophila melanogaster: implication of ER stress, autophagy, and apoptosis. [FBrf0220377] Fredriksson et al., 2012, Aging Cell 11(4): 634--643 Effects of aging and reproduction on protein quality control in soma and gametes of Drosophila melanogaster. [FBrf0218923] Yano et al., 2012, J. Neurogenet. 26(1): 53--63 Identification of proteasome components required for apical localization of chaoptin using functional genomics. [FBrf0217843] Booker et al., 2011, BMC Genomics 12: 50 False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...