STAT, marelle, mrl, D-STAT, Dstat
transcription factor - cytoplasmic signal transducing protein - regulates the even-skipped stripe 3 promoter and the pair rule gene runt - central to the establishment of planar polarity during Drosophila eye development
Please see the JBrowse view of Dmel\Stat92E for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
4.0 (northern blot)
4 (northern blot)
The group(s) of polypeptides indicated below share identical sequence to each other.
761 (aa); 86 (kD predicted)
754 (aa)
Forms a homodimer or a heterodimer with a related family member.
Tyrosine phosphorylated by hopscotch. Phosphorylation is required for DNA-binding activity and dimerization.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Stat92E using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: maternally deposited
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as procephalic ectoderm primordium
Comment: reported as dorsal epidermis anlage
Comment: reported as head epidermis primordium
Comment: reported as head epidermis primordium
Comment: reported as head epidermis primordium
Expression pattern inferred from unspecified enhancer trap line.
Stat92E transcripts are detected at all stages on northern blots. They are detected at high and uniformly distributed levels by in situ hybridization in early syncytial and cellularizing embryos. During germ band extension they are detected in a striped pattern within every segment.
Stat92E transcripts are detected at all stages of development by northern blot. Stat92E transcripts are detected in very early embryos in a uniform pattern by in situ hybridization. At the blastoderm stage, expression is seen in seven stripes in a broad central domain as well as in clusters of cells in anterior and posterior terminal segments. At germ band extension, 14 stripes are seen, restricted to mesodermal tissue. After germ band retraction, expression is observed mainly in the foregut, the hingut and in gonadal precursor cells.
Comment: male
Comment: hub-proximal
At the third instar larval stage, Stat92E protein accumulates in the nucleus in the wing hinge and the anterior border of the notum. It accumulates in the cytoplasm in the wing pouch and most of the notum.
Stat92E is expressed widely in the adult brain. It localizes to Kenyon cell bodies and is also present in subdomains of the calyx. It is present in mushroom body cell bodies.
Stat92E protein is expressed the larval outer optic anlage from the second through late third larval instar. Expression is higher in in the lateral neuroepithelium, decreasing in medial cells.
Stat92E protein is expressed in ISCs and enteroblasts.
In testes, Stat92E protein is detected at higher levels in germline stem cells than in cyst progenitor cells.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Stat92E in JBrowsePlease Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
DNA-protein interactions: genome-wide binding profile assayed for Stat92E protein in 0-12 hr embryos; see mE1_TFBS_Stat92E collection report.
dsRNA made from templates generated with primers directed against this gene.
dsRNA made from templates generated with primers directed against this gene used in a cell-based RNAi assay to identify components or modifiers of the JAK/STAT pathway.
Treatment of S2-derived S2-NP cells with dsRNA made from templates generated with primers directed against Stat92E results in a 12-24-fold decrease in JAK/STAT activity.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Stat92E is involved in proper differentiation and morphogenesis of multiple tissues.
Stat92E function is necessary for the proper differentiation of the polar follicle cells and the interfollicle cells. The failure of these cell to differentiate correctly leads to fused egg chambers and results in female sterility. Stat92E is part of an intracellular Jak-Stat signalling pathway and is activated by the hop Jak kinase. Partial loss of hop gene product activity gives a phenotype similar to that of Stat92E mutants, supporting the idea that the Jak-Stat pathway is involved in regulation of oogenesis.
Stat92E is required autonomously in the germ cells for germline stem cell maintenance.
Stat92E is required for border cell migration.
Stat92E is required in the male germline for maintenance of germ line stem cell renewal.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.
A unique binding activity that resembles the Stat-like activity of the mammalian system is identified in Drosophila and is encoded by pp100 and pp150 polypeptides. Vanadate/hydrogen peroxide treatment of Schneider cells induces a specific GRR binding complex whose formation is dependent upon Tyr phosphorylation.
Source for identity of: Stat92E CG4257
"marelle" is French for "hopscotch".