A Database of Drosophila Genes & Genomes

FB2013_03, released May 7th, 2013
 

Gene Tn10\tetR

General Information
SymbolTn10\tetRSpeciesb. Transposon Tn10
Nametetracyclin repressorAnnotation symbol
Feature typeengineered_foreign_geneFlyBase IDFBgn0016944
Gene Model StatusNot Applicable Stock availability 4 publicly available
Also Known AstetR, tTA, rtTA
Genomic Location
Chromosome (arm)Recombination map
Cytogenetic mapSequence location
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Automatically generated summary

See sections below for more information
The gene tetracyclin repressor is referred to in FlyBase by the symbol Tn10\tetR (FBgn0016944). It is a engineered_foreign_gene from bacterial Transposon Tn10. Its molecular function is unknown. The biological processes in which it is involved are not known. 15 alleles are reported. The phenotypes of these alleles are annotated with: larval head; dorsal closure embryo; eye; pupa; adult abdomen; wing. It has no annotated transcripts. Gene has not been localized to the genome sequence.
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Description
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FB2013_03
FB2013_02
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FlyBase Computed Cytological Location
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Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map
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Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
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Annotated Transcripts
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RefSeq ID
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Annotated Polypeptides
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Length (aa)
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RefSeq ID
GenBank protein
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DDBJ /
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DNA sequence
Protein sequence
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UniProtKB/Swiss-Prot
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Additional Descriptive Data
Marker for
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Marker for
Subcellular Localization (GO Cellular Component)
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Reference
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
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Lethality
Allele
lethal, with ash1PdL11A3
lethal, with CaMKIIT287D.Tn10\tetO, Scer\GAL429BD
lethal, with CaMKIIT287D.Tn10\tetO, Scer\GAL4elav-C155
lethal, with CaMKIIT287D.Tn10\tetO, Scer\GAL4MJ85b
lethal, with PdL2B1PdL2B1
lethal, with PdL3APdL3A
lethal, with PdL14B1PdL14B1
lethal, with PdL43C1PdL43C1
lethal, with PdL45C1PdL45C1
lethal, with PdL72A3PdL72A3
lethal, with PdL73A1PdL73A1
lethal, with PdL75A3PdL75A3
lethal, with Ras64BV12.Tn10\tetO
lethal | female, with msl-2NOPU.Tn10\tetO
lethal | female, with Ras64BV12.Tn10\tetO
lethal | female limited, with WTn10\tetO.cHa
lethal | P-stage, with Hsap\UBBTn10\tetO.cHa
lethal | tetracycline conditional, with Scer\GAL4salm-459.2, rprTn10\tetO.cSa
Sterility
Allele
female semi-sterile, with MRG15dsRNA.Tn10\tetO
female semi-sterile, with MRG15MT1.Tn10\tetO.T:Ivir\HA1
Other Phenotypes
Allele
courtship behavior defective, with CaMKIIT287D.Tn10\tetO, Scer\GAL430Y
courtship behavior defective, with CaMKIIT287D.Tn10\tetO, Scer\GAL4MJ85b
courtship behavior defective | male | tetracycline conditional, with CaMKIIT287D.Tn10\tetO, Scer\GAL430Y
courtship behavior defective | male | tetracycline conditional, with CaMKIIT287D.Tn10\tetO, Scer\GAL430Y, Scer\GAL80Cha.PK
heat stress response defective | tetracycline conditional, with Hsp22Tn10\tetO.cBa
increased cell death | P-stage, with Hsap\UBBTn10\tetO.cHa
long lived, with Cct1PdL8R128
long lived, with cherPdL8S64
long lived, with fwdPdL8S25
long lived, with RdhPdL3E36
long lived, with Scer\GAL4D42, hebeTn10\tetO.cLa
long lived, with Scer\GAL4D42, maguTn10\tetO.cLa
long lived, with Sod2Tn10\tetO.cCa
long lived, with SugPdL2C33
long lived, with VhaSFDPdL4G14
long lived, with α-Man-IIPdL19B3
long lived | male limited, with Hsap\UBBTn10\tetO.cHa, Scer\GAL4da.G32
melanotic mass phenotype | P-stage, with Hsap\UBBTn10\tetO.cHa
oxidative stress response defective | tetracycline conditional, with Hsp22Tn10\tetO.cBa
short lived, with ash1PdL11A3
short lived, with MRG15dsRNA.Tn10\tetO
short lived, with PdL2B1PdL2B1
short lived, with PdL14B1PdL14B1
short lived, with PdL35B1PdL35B1
short lived, with PdL43C1PdL43C1
short lived, with pumPdL58A2
short lived | :heat sensitive | tetracycline conditional, with Hsp22Tn10\tetO.cBa
short lived | heat sensitive, with Sod2Tn10\tetO.cCa
short lived | male limited, with Hsap\UBB+1.Tn10\tetO, Scer\GAL4da.G32
short lived | tetracycline conditional, with Sod2Tn10\tetO.cFa
small body, with CaMKIIT287D.Tn10\tetO, Scer\GAL4MJ85b
visible, with PdL35B1PdL35B1
visible, with pumPdL3B2
visible, with pumPdL3G
visible, with pumPdL58A2
visible, with α-Man-IIPdL19B3
visible | tetracycline conditional, with Scer\GAL4salm-459.2, rprTn10\tetO.cSa
wild-type, with ScrDD.Tn10\tetO.T:Ivir\HA1
Phenotype manifest in
Allele
adult abdomen, with CaMKIIT287D.Tn10\tetO, Scer\GAL429BD
chorion | drug conditional, with foxoY2-26
chorion | drug conditional, with ush3-38
dorsal appendage | drug conditional, with dbr3M1753
dorsal appendage | drug conditional, with edl4M127
dorsal appendage | drug conditional, with foxoY2-26
dorsal appendage | drug conditional, with wmdpter-2-4
dorsal closure embryo, with ScrAA.Tn10\tetO.T:Ivir\HA1
dorsal closure embryo, with ScrTn10\tetO.T:Ivir\HA1
embryonic head segment | dorsal, with AntpTn10\tetO.cBa
eye, with AntpTn10\tetO.cBa
eye, with α-Man-IIPdL19B3
eye | tetracycline conditional, with Scer\GAL4salm-459.2, rprTn10\tetO.cSa
larval head, with ScrAA.Tn10\tetO.T:Ivir\HA1
larval head, with ScrTn10\tetO.T:Ivir\HA1
micropyle | drug conditional, with dbr3M1753
prothoracic ventral denticle belt | embryonic stage, with AntpTn10\tetO.cBa
pupa, with Hsap\UBBTn10\tetO.cHa
salivary gland | ectopic, with ScrAA.Tn10\tetO.T:Ivir\HA1
salivary gland | ectopic, with ScrTn10\tetO.T:Ivir\HA1
wing, with CaMKIIT287D.Tn10\tetO, Scer\GAL429BD
wing, with PdL35B1PdL35B1
wing, with pumPdL3B2
wing, with pumPdL3G
wing, with pumPdL58A2
wing | tetracycline conditional, with Scer\GAL4salm-459.2, rprTn10\tetO.cSa
hide Classical Alleles ( 0 )
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Allele of Tn10\tetRClassMutagenStocksKnown lesion
hide Alleles Carried on Transgenic Constructs ( 15 )
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Allele of Tn10\tetRClassMutagenStocksKnown lesion
Tn10\tetRhs.T:Hsim\VP162 Yes
Tn10\tetRey.3.5.T:Hsim\VP161 Yes
Tn10\tetRScer\UAS.T:Hsim\VP161 Yes
Tn10\tetRAct5C.PR0 Yes
Tn10\tetRAct5C.T:Hsim\VP160 Yes
Tn10\tetRalt.Act5C.T:Hsim\VP160 Yes
Tn10\tetRHsp26.T:Hsim\VP160 Yes
Tn10\tetRM2.alt.Act5C.T:Hsim\VP160 Yes
Tn10\tetRM2.alt.Scer\UAS.T:Hsim\VP160 Yes
Tn10\tetRMmus\Hoxa7.630.T:Hsim\VP160 Yes
Tn10\tetRNLS.nos.T:Avic\GFP-EGFP0 Yes
Tn10\tetRP\T.PH0 Yes
Tn10\tetRRHT.T:Hsim\VP160 Yes
Tn10\tetRYp1.hs0 Yes
Tn10\tetRYp3.T:Hsim\VP160 Yes
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Transgenic Constructs
Type of construct
Name
Expression data
Insertions
Type of insertions
Name
Expression data
hide Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )
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hide Sequence Ontology: Class of Gene
engineered_foreign_gene
 
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Interacts with
Please look at the allele data for full details of the genetic interactions
Tn10\tetR allele
Gene
References
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hide OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
OrthoDB orthology group searches
(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)
Drosophila inclusive ortholog search
No orthologs identified
Dipteran inclusive ortholog search
No orthologs identified
Insect inclusive ortholog search
No orthologs identified
Arthropod inclusive ortholog search
No orthologs identified
Metazoa inclusive ortholog search
No orthologs identified
Ortholog(s) in Drosophila melanogaster (None identified)
No D. melanogaster orthologies identified
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Gene
OMIM
HGNC
hideAAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
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hide Stocks Listed in FlyBase ( 4 )
Bloomington
26801
P{ey-tTA}3, ry506
27891
y1 w*; P{UAS-tTA.S}4
26802
y1 w*; P{Hsp70.tTA.Ba}2
26803
y1 w*; P{Hsp70.tTA.Ba}3
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A component of the tetracyclin (tet)-regulated transactivation system: Tn10\tetR-regulated transactivating protein under the control of regulatory sequences is crossed to a strain containing the coding sequence for a gene of interest downstream of the Tn10\tetO operator sequences. Expression of the gene can be tightly controlled by Tn10\tetR. This system is used to study SAR function in vivo, a synthetic SAR-binding protein is overexpressed by means of the Tet system.
(Girard et al., 1998)
hide Tag or Foreign Gene Data
  • Foreign sequence; species == bacterial transposon; gene == 'tetR, tetracyclin repressor'; EMBL:X00694.
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hide Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym
pSC101\tetR
 
rtTA
(Landis et al., 2001, Khokhar et al., 2004, Li and Tower, 2009, Curtis et al., 2007)
tetR
(Alphey, 2002, Schuh et al., 2007, Schuh et al., 2007)
TetR
(Ryu et al., 2001, Nilsson et al., 2000, Hoe et al., 2011)
Tn10\tetR
 
tTA
(Horn et al., 2003, Stebbins and Yin, 2001, Bello et al., 1998, Horn et al., 2002, Mehren and Griffith, 2004)
tTa
(Thomas et al., 2000)
Name Synonym
tetracyclin repressor
 
Secondary FlyBase IDs
  • FBgn0024597
hide References ( 34 )
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hide Recent research papers ( 1 )
Hoe et al., 2011, Aging 3(3): 237--261
Ubiquitin over-expression phenotypes and ubiquitin gene molecular misreading during aging in Drosophila melanogaster. [FBrf0213373]
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