poc, l(2)03350, rno, l(2)k07612, l(2)k06805
codes for RRM motif RNA-binding protein - mutants have defects in Notch and Egf receptor signaling resulting defects in cell-fate and in axon guidance - large and small SPEN family proteins stimulate axon outgrowth during neurosecretory cell remodeling
Please see the JBrowse view of Dmel\spen for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.44
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.45
Gene model reviewed during 5.56
20 (northern blot)
5476 (aa)
5533, 5476 (aa); 597 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\spen using the Feature Mapper tool.
Comment: maternally deposited
Comment: reference states 0-24 hr AEL
Comment: reference states 2-24 hr AEL
spen proteins are expressed in most cell types starting in stage 3 embryonic nuclei. Expression continues through the rest of embryogenesis, and is concentrated in nuclei. Higher levels of expression are detected in the epidermis and central nervous system in stage 9-14 embryos.
GBrowse - Visual display of RNA-Seq signals
View Dmel\spen in GBrowse 22-0.5
2-0.0
2-1.0 +/- 0.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Source for merge of: poc E(Raf)2A
Source for merge of: spen poc l(2)k06805 l(2)03350 l(2)k07612 l(2)k08102
Source for merge of: spen CG18497
Source for merge of: spen yip1
Source for merge of: spen rno
Source for merge of: spen BcDNA:GM01870
Source for merge of spen BcDNA:GM01870 was a shared cDNA ( date:030728 ).
ChEST reveals this is a target of Mef2.
spen appears to be required to maintain multicellular organization.
spen regulates development of midline glial cells.
Maternally contributed spen protein is required for the specification of cell fate during neuronal and midline development.
Identification: Identified on the basis of interaction with aop mutations.
Mutants show a homeotic phenotype developing head-like skeletal features in the trunk.
spen does not repress ectopic head-like cuticle by repressing the expression or function of known head-determining genes. Overall orientation not stated: anon-21Ab+ spen+ kis-
Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.
Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.
Identified in a genetic screen for modifiers of the phl::tor12D.sev rough eye mutant phenotype.
The ventral naked cuticle of T2, T3 and occasionally A1 of spen- embryos shows an apparent transformation towards head structures.
Identified in a screen for genes whose function is required for the function of Dfd.
