FlyBase Gene Report: Dmel\smid

General Information

Symbol

Dmel\smid

Species

D. melanogaster

Name

smallminded

Annotation Symbol

CG8571

Feature Type

protein_coding_gene

FlyBase ID

FBgn0016983

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

AAA-protein family - ATPase - Mutants are defective in larval mitoses - expression of Smallminded in cells that undergo late embryonic or larval mitosis suggests a general role in promoting mitosis (Interactive Fly, smid )

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(3)j6B8

Key Links

Genomic Location

Cytogenetic map

65F9-65F10

Sequence location

3L:7,381,614..7,385,637 [+]

Recombination map

3-20

RefSeq locus

NT_037436 REGION:7381614..7385637

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960

(InterPro Project Members, 2004-)

enables ATP hydrolysis activity

inferred from electronic annotation with InterPro:IPR003593, InterPro:IPR003959, InterPro:IPR003960

(InterPro Project Members, 2004-)

inferred from electronic annotation with InterPro:IPR003593, InterPro:IPR003959, InterPro:IPR055278

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN004603074

(GO Reference Genome Project, 2011-)

enables preribosome binding

inferred from biological aspect of ancestor with PANTHER:PTN000554586

(GO Reference Genome Project, 2011-)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in ribosome biogenesis

inferred from biological aspect of ancestor with PANTHER:PTN000554586

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000554586

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

UNCLASSIFIED ATPASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

ATP hydrolysis activity
RHEA 13065:

Summaries

Gene Group (FlyBase)

UNCLASSIFIED ATPASES -: This group comprises ATPases that do not classify under other groups in FlyBase.

Summary (Interactive Fly)

AAA-protein family - ATPase - Mutants are defective in larval mitoses - expression of Smallminded in cells that undergo late embryonic or larval mitosis suggests a general role in promoting mitosis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\smid for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VS62)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.45

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.39

Gene model reviewed during 5.46

Gene model reviewed during 5.55

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

smid-RA

FBtr0076851

NM_079235

3170

944

smid-RC

FBtr0301611

NM_001169900

3167

943

smid-RG

FBtr0331849

NM_001274583

3313

910

smid-RH

FBtr0331850

NM_001274584

3119

927

smid-RI

FBtr0333404

NM_206287

3080

910

Additional Transcript Data and Comments

Reported size (kB)

3.2 (northern blot)

(Long et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

smid-PA

FBpp0089153

104.4

944

5.09

FBpp0290826

104.3

943

5.09

FBpp0304233

100.3

910

4.91

FBpp0304234

102.6

927

5.02

FBpp0305596

100.3

910

4.91

Q7KU82

NP_996009

AAS65065

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

910 aa isoforms: smid-PG, smid-PI

Additional Polypeptide Data and Comments

Reported size (kDa)

943 (aa)

(Long et al., 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\smid using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.41

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

fat body/gonad primordium

(Fisher et al., 2012)

germline cell

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Long et al., 1998)

Additional Descriptive Data

smid transcripts are detected in all phases of the life cycle with the highest levels in embryos.

(Long et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

Reporter: P{GawB}smid^C161

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

Bolwig nerve

(Renn et al., 1999)

sensory neuron

(Long et al., 1998)

imaginal disc

(Long et al., 1998)

neuroblast

(Long et al., 1998)

larval central nervous system | restricted

(Long et al., 1998)

first instar larval stage

larval peripheral nervous system | restricted

(Williams and Shepherd, 1999)

second instar larval stage -- third instar larval stage

larval thoracic lateral multidendritic neuron ldaB

(Nagel et al., 2012)

pupal stage P7 -- adult stage

small field neuron

Comment: expression starts 32 hr APF

(Renn et al., 1999)

pharate adult stage

peripheral nervous system | restricted

(Williams and Shepherd, 1999)

adult stage

adult protocerebral bridge 1 glomerulus-ellipsoid body tile-nodulus 1 neuron

(Renn et al., 1999)

ellipsoid body | restricted

(Renn et al., 1999)

protocerebral bridge | restricted

(Renn et al., 1999)

nodulus | restricted

(Renn et al., 1999)

leg

(Smith and Shepherd, 1996)

mesothoracic femoral chordotonal organ

(Williams et al., 2000, Smith and Shepherd, 1996)

metathoracic femoral chordotonal organ

(Williams et al., 2000, Smith and Shepherd, 1996)

prothoracic femoral chordotonal organ

(Williams et al., 2000, Smith and Shepherd, 1996)

sensory neuron of tibia | subset

Comment: Located at the tibial-tarsal joint

(Smith and Shepherd, 1996)

sensory neuron of metatarsus | subset

Comment: Located at the distal joint.

(Smith and Shepherd, 1996)

sensory neuron of tarsal segment 2 | subset

Comment: Located at the distal joint.

(Smith and Shepherd, 1996)

sensory neuron of tarsal segment 3 | subset

Comment: Located at the distal joint.

(Smith and Shepherd, 1996)

sensory neuron of tarsal segment 4 | subset

Comment: Located at the distal joint.

(Smith and Shepherd, 1996)

hair plate of prothoracic trochanter

(Smith and Shepherd, 1996)

hair plate of prothoracic coxa

(Smith and Shepherd, 1996)

femoral campaniform sensillum | subset

(Smith and Shepherd, 1996)

wing

(Smith and Shepherd, 1996)

campaniform sensillum of dorsal radius Sc4d

(Smith and Shepherd, 1996)

campaniform sensillum of dorsal radius Sc25

(Smith and Shepherd, 1996)

campaniform sensillum of dorsal radius Sc12

(Smith and Shepherd, 1996)

campaniform sensillum of ventral radius Sc4v

(Smith and Shepherd, 1996)

sensillum of tegula

(Smith and Shepherd, 1996)

campaniform sensillum of wing vein L3 | subset

(Smith and Shepherd, 1996)

haltere sensillum

(Smith and Shepherd, 1996)

adult abdomen

(Smith and Shepherd, 1996)

Wheeler's organ

(Smith and Shepherd, 1996)

sensory neuron of adult abdomen | subset

(Smith and Shepherd, 1996)

larval abdominal ventral cluster group multidendritic neuron | subset

(Smith and Shepherd, 1996)

larval abdominal lateral multidendritic neuron | subset

(Smith and Shepherd, 1996)

sensory neuron of central nervous system | subset

Comment: Neuron is probably mechanosensory

(Smith and Shepherd, 1996)

adult anterior dorsal mesothoracic nerve

(Smith and Shepherd, 1996)

sensory neuron of adult prothoracic segment

(Smith and Shepherd, 1996)

abdominal sensory neuron | subset

(Smith and Shepherd, 1996)

bipolar dendrite neuron

(Smith and Shepherd, 1996)

prothoracic neuromere | restricted

(Williams et al., 2000)

mesothoracic leg hair plate

(Williams et al., 2000)

metathoracic leg hair plate

(Williams et al., 2000)

prothoracic leg hair plate

(Williams et al., 2000)

campaniform sensillum | subset of leg

(Williams et al., 2000)

abdominal dorsal multidendritic neuron ddaC

(Shimono et al., 2009)

abdominal dorsal multidendritic neuron ddaD

(Shimono et al., 2009)

abdominal dorsal multidendritic neuron ddaE

(Shimono et al., 2009)

larval abdominal lateral multidendritic neuron ldaA

(Shimono et al., 2009)

abdominal anterior ventral multidendritic neuron vdaa

(Shimono et al., 2009)

adult abdominal lateral multidendritic neuron ldaA-like

(Shimono et al., 2009)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\smid in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\smid

View exonic expression by tissue for Dmel\smid

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016983

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38824/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016983

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016983

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016983

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8571

Flygut - An atlas of the Drosophila adult midgut

FBgn0016983

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\smid

Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 14 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of smid

Transgenic constructs containing regulatory region of smid

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3L)BSC875

(Cook and Cook, 2010.5.7)

Df(3L)Exel8104

(Ryder, 2004.4.26)

Df(3L)pbl-X1

(Spradling et al., 1999, Long et al., 1998, Long et al., 1998, BDGP Project Members, 1994-1999)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^insc-Mz1407

smid^GD13972

lethal | recessive

lethal - all die before end of larval stage | recessive

smid^C161

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

smid^GD13972

viable, with Scer\GAL4^pnr-MD237

smid^GD13972

viable, with Scer\GAL4^tin.CΔ4

smid^GD13972

Other Phenotypes

Allele

abnormal locomotor rhythm | recessive

smid^C161

abnormal neuroanatomy | larval stage, with Scer\GAL4^insc-Mz1407

smid^GD13972

decreased cell number | somatic clone | third instar larval stage

decreased occurrence of cell division | larval stage

smid^C161

increased cell size | larval stage

smid^C161

some die during larval stage | recessive

smid^C161

visible, with Scer\GAL4^pnr-MD237

smid^GD13972

Phenotype manifest in

Allele

central nervous system

smid^C161

ganglion mother cell | larval stage, with Scer\GAL4^insc-Mz1407

smid^GD13972

larval brain

smid^C161

larval brain | somatic clone | third instar larval stage

larval central nervous system

smid^C161

neuroblast | larval stage

smid^C161

trichogen cell, with Scer\GAL4^pnr-MD237

smid^GD13972

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (33)

Hsap\NVL

12 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (21)

Rnor\Nvl

13 of 14

Yes

4 of 14

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (21)

Mmus\Nvl

12 of 14

Yes

4 of 14

4 of 14

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (27)

Xtro\nvl

6 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

1 of 13

1 of 13

Yes

Xtro\atad2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\pex6

1 of 13

Xtro\prxl2a

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (25)

Drer\nvl

13 of 14

Yes

4 of 14

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Drer\si:dkey-195m11.8

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (25)

Cele\mac-1

12 of 14

Yes

4 of 14

4 of 14

4 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (32)

Agam\AgaP_AGAP006205

12 of 12

Yes

4 of 12

4 of 12

3 of 12

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (84)

Atha\CDC48C

11 of 13

Yes

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

Atha\AT4G04180

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (21)

Scer\RIX7

12 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Schizosaccharomyces pombe (Fission yeast) (15)

Spom\SPBC16E9.10c

11 of 12

Yes

3 of 12

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Spom\SPBC947.01

1 of 12

Spom\SPCC24B10.10c

1 of 12

Spom\SPCC965.04c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:smid. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (32)

6 of 13

6 of 13

6 of 13

5 of 13

3 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

bor

1 of 13

CG4701

1 of 13

CG10793

1 of 13

nmd

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

smid^j6B8

ameliorates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

smid^NIG.8571R

exacerbates frontotemporal dementia and/or amyotrophic lateral sclerosis 1

modeled by Zzzz\poly-GR^PO-36.UAS

(Park et al., 2021)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NVL; nuclear VCP like

12 of 14

602426

AFG2A; AFG2 AAA ATPase homolog A

4 of 14

613940

NEURODEVELOPMENTAL DISORDER WITH HEARING LOSS, SEIZURES, AND BRAIN ABNORMALITIES; NEDHSB

AFG2B; AFG2 AAA ATPase homolog B

4 of 14

619578

VCP; valosin containing protein

4 of 14

601023

PEX1; peroxisomal biogenesis factor 1

3 of 14

602136

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

smid - Akt

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - Myc

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - Tsc1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - kel

proximity-dependent biotin identification, Identification by mass spectrometry

(Mannix et al., 2019)

smid - rictor

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

smid - sima

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64263

DroID - A comprehensive database of gene and protein interactions.

FBgn0016983

MIST (protein-protein) - An integrated Molecular Interaction Database

38824

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome biogenesis in eukaryotes

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-20

Cytogenetic map

65F9-65F10

Sequence location

3L:7,381,614..7,385,637 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65F9-65F10

Limits computationally determined from genome sequence between P{PZ}l(3)07217⁰⁸²²³&P{EP}Rac2^EP3118 and P{lacW}Nmt^j1C7

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66A1-66A22

(Long, 1996.7.5)

66A1-66A2

(determined by in situ hybridisation)

(Spradling et al., 1999)

66A-66A

(determined by in situ hybridisation)

(Long et al., 1998)

66A1-66A2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

3-20

(FlyBase, 2016)

3-17.1

(Comeron et al., 2012)

(Long, 1996.7.5)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

27893

w^*; P{GawB}smid^C161/TM6B, Tb¹

12070

y¹ w^*; P{lacW}smid^j6B8/TM3, Sb¹

36808

y¹ sc^* v¹ sev²¹; P{TRiP.GL01024}attP40

35704

y¹ sc^* v¹ sev²¹; P{TRiP.GLV21069}attP2/TM3, Sb¹

Kyoto

111382

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}smid^j6B8 P{neoFRT}80B/TM6B, P{Car20y}TPN1, Tb¹

8571R-3

v108178

v35965

w¹¹¹⁸; P{GD13972}v35965/TM3

Genomic Clones (16)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (107)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE47719

Other clones

MIP20544

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0016983

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0016983

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: smid l(3)j6B8

(Spradling et al., 1999)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Gene is named 'smallminded' due to the reduced central nervous system phenotype of mutants.

(Long et al., 1998)

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

CG8571

(Çiçek et al., 2016, Hosono et al., 2015, Ding et al., 2014, Bridon et al., 2012, Christis and Munro, 2012, Ni et al., 2011.7.19, Keleman et al., 2009.8.5)

Smid

(David-Morrison et al., 2016, Kwong et al., 2015, Anholt and Williams, 2010)

l(3)j6B8

(BDGP Project Members, 1994-1999)

l(3)j8A6

l(3)s2898

smid

(Park et al., 2021, Sureka and Mishra, 2021, Mannix et al., 2019, Dent et al., 2015, Kwong et al., 2015, Japanese National Institute of Genetics, 2012.5.21, Ambegaokar and Jackson, 2011, Friedman et al., 2011, Cook and Cook, 2010.5.7, Svensson et al., 2007, Altenhein et al., 2006, Slack et al., 2006)

Name Synonyms

small-minded

(Bridon et al., 2012, Slack et al., 2006)

smallminded

(Long et al., 1998, Long, 1996.7.5)

Secondary FlyBase IDs

FBgn0011034
FBgn0011393
FBgn0011406
FBgn0024837

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 61 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38824

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VS62

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VS62

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0016983

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38824/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0016983

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0016983

FlyMine - An integrated database for Drosophila genomics

FBgn0016983

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG8571

MARRVEL_MODEL - MARRVEL (model organism gene)

38824

Linkouts

BioGRID - A database of protein and genetic interactions.

64263

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0016983

DroID - A comprehensive database of gene and protein interactions.

FBgn0016983

DRSC - Results frm RNAi screens

FBgn0016983

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0016983

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0016983

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG8571

Flygut - An atlas of the Drosophila adult midgut

FBgn0016983

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002784

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Ribosome biogenesis in eukaryotes

MIST (protein-protein) - An integrated Molecular Interaction Database

38824

References (72)

The following external sites may use different assemblies or annotations than FlyBase.

-0.41

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