U2AF, dU2AF38, dU2AF38
Gene model reviewed during 5.44
Gene model reviewed during 5.46
264 (aa); 30 (kD predicted)
Associates with a 65 kDa protein.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\U2af38 using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\U2af38 in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: U2af38 CG3582
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in a long metaphase spindle with misaligned chromosomes when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
Studies in vivo reveal that the RS domain of U2af38 is completely dispensable: the domain is not necessary for constitutive splicing in vitro. The RS domain is also not required for enhancer-dependent splicing of dsx in vitro.
E.coli copurification assay is used to map the domain on U2af50 that interacts with U2af38 : a 28 amino acid fragment is necessary and sufficient for the interaction. Interaction studies in vivo indicate that the U2af50/U2af38 heterodimer is essential for viability in vivo.