General Information Symbol Dmel\ RpS3ASpecies D. melanogaster Name Ribosomal protein S3A Annotation symbol CG2168 Feature type protein_coding_gene FlyBase ID FBgn0017545 Gene Model Status Current Stock availability 5 publicly
available Also Known As M(4)101, C3, M(4)57g Genomic Location Chromosome (arm) 4 Recombination map 4- Cytogenetic map 101F1-101F1 Sequence location 4:85,851..87,833 [-]
Genomic Maps
Summary Information Automatically generated summary
See sections below for more information The gene
Ribosomal protein S3A is referred to in FlyBase by the symbol
Dmel\RpS3A (CG2168, FBgn0017545). It is a protein_coding_gene from
Drosophila melanogaster . Based on sequence similarity, it is predicted to have
molecular function : structural constituent of ribosome. There is experimental evidence that it is involved in the
biological process : mitotic spindle elongation; mitotic spindle organization.
7 alleles are reported . The
phenotypes of these alleles are annotated with: adult; macrochaeta; adult heart. It has
3 annotated transcripts and
3 annotated polypeptides .
Protein features are: Ribosomal protein S3Ae; Ribosomal protein S3Ae, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1638439_a_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of RpS3A. Gene sequence location is
4:85851..87833 .
User Contributed Data
Phenotypic Description from the Red Book (Lindsley
& Zimm 1992) Gene/Allele symbols may differ
from current usage M(4)101
Medium Minute; viability good; development protracted; eclosion delayed 33 hr (Ferrus, 1975, Genetics
79: 589-99). M(4)101/+/+ triplo-fours are non-Minute (Mohr,
1933, Hereditas 17: 317-32). Homozygous mutants die in
embryonic stage (Farnsworth, 1951, Genetics 36: 550). RK2.
Recent Updates
Description
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Detailed Mapping Data
FlyBase Computed Cytological Location
Experimentally Determined Cytological Location
101F-101F
(determined by in situ hybridisation)
101F-101F
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location Left of (cM) Right of (cM) Notes Gene Model & Products Comments on Gene
Model Gene model reviewed during 5.39
Low-frequency RNA-Seq exon junction(s) not annotated.
Multiphase exon postulated: reading frame of first coding exon differs in alternative transcripts.
Gene model reviewed during 5.46
Transcript Data Annotated Transcripts Additional Transcript Data
& Comments Reported size (kB) Comments External Data Crossreferences
Polypeptide Data Annotated
Polypeptides Additional Polypeptide
Data & Comments Reported size (kDa) Comments External Data Linkouts Crossreferences
domains - A database of protein families, domains, and functional sites
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank UniProtKB/Swiss-Prot UniProtKB/TrEMBL Mapped Features Mapped Features have been reorganized, please
see this article for details. Additional mapped features and mutations can
be found on GBrowse or related reports. External Data Linkouts Crossreferences Expression Data Transcript Expression
Additional Descriptive Data
RpS3A transcripts are expressed in all stages tested. Expression is ubiquitous in embryos from the early cleavage stages through germ band extension.
Marker for Subcellular Localization CV Term Polypeptide Expression
Additional Descriptive Data
Marker for Subcellular Localization (GO Cellular Component) Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference
FlyAtlas Anatomy Microarray
Linear, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
3080.95
Larval Midgut
5226.1
Larval Hindgut
5141.1
Larval Malpighian Tubules
6411.1
Larval Fat Body
8869.3
Larval Salivary Gland
9397
Larval Trachea
7494.95
Larval Carcass
5633.7
Adult Head
5215.4
Adult Eye
8606.425
Adult Brain
3475.1
Adult Thoracic-Abdominal Ganglion
4947.2
Adult Crop
7390.3
Adult Midgut
6065.4
Adult Hindgut
5834.5
Adult Malpighian Tubules
7264.7
Adult Fat Body
6351.8
Adult Salivary Gland
14913.8
Adult Heart
8450.4
Adult VirginFemale Spermatheca
6446.4
Adult InseminatedFemale Spermatheca
6648.4
Adult Ovary
4338.5
Adult Testis
1713.8
Adult Male Accessory Gland
11197.2
Adult Carcass
6695.1
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
(3080.95)
Larval Midgut
(5226.1)
Larval Hindgut
(5141.1)
Larval Malpighian Tubules
(6411.1)
Larval Fat Body
(8869.3)
Larval Salivary Gland
(9397)
Larval Trachea
(7494.95)
Larval Carcass
(5633.7)
Adult Head
(5215.4)
Adult Eye
(8606.425)
Adult Brain
(3475.1)
Adult Thoracic-Abdominal Ganglion
(4947.2)
Adult Crop
(7390.3)
Adult Midgut
(6065.4)
Adult Hindgut
(5834.5)
Adult Malpighian Tubules
(7264.7)
Adult Fat Body
(6351.8)
Adult Salivary Gland
(14913.8)
Adult Heart
(8450.4)
Adult VirginFemale Spermatheca
(6446.4)
Adult InseminatedFemale Spermatheca
(6648.4)
Adult Ovary
(4338.5)
Adult Testis
(1713.8)
Adult Male Accessory Gland
(11197.2)
Adult Carcass
(6695.1)
Expression Level Scale
None
Low
Moderate
High
Linear, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
(3080.95)
Larval Midgut
(5226.1)
Larval Hindgut
(5141.1)
Larval Malpighian Tubules
(6411.1)
Larval Fat Body
(8869.3)
Larval Salivary Gland
(9397)
Larval Trachea
(7494.95)
Larval Carcass
(5633.7)
Adult Head
(5215.4)
Adult Eye
(8606.425)
Adult Brain
(3475.1)
Adult Thoracic-Abdominal Ganglion
(4947.2)
Adult Crop
(7390.3)
Adult Midgut
(6065.4)
Adult Hindgut
(5834.5)
Adult Malpighian Tubules
(7264.7)
Adult Fat Body
(6351.8)
Adult Salivary Gland
(14913.8)
Adult Heart
(8450.4)
Adult VirginFemale Spermatheca
(6446.4)
Adult InseminatedFemale Spermatheca
(6648.4)
Adult Ovary
(4338.5)
Adult Testis
(1713.8)
Adult Male Accessory Gland
(11197.2)
Adult Carcass
(6695.1)
Expression Level Scale
None
Low
Moderate
High
Very high
Linear, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
3080.95
Larval Midgut
5226.1
Larval Hindgut
5141.1
Larval Malpighian Tubules
6411.1
Larval Fat Body
8869.3
Larval Salivary Gland
9397
Larval Trachea
7494.95
Larval Carcass
5633.7
Adult Head
5215.4
Adult Eye
8606.425
Adult Brain
3475.1
Adult Thoracic-Abdominal Ganglion
4947.2
Adult Crop
7390.3
Adult Midgut
6065.4
Adult Hindgut
5834.5
Adult Malpighian Tubules
7264.7
Adult Fat Body
6351.8
Adult Salivary Gland
14913.8
Adult Heart
8450.4
Adult VirginFemale Spermatheca
6446.4
Adult InseminatedFemale Spermatheca
6648.4
Adult Ovary
4338.5
Adult Testis
1713.8
Adult Male Accessory Gland
11197.2
Adult Carcass
6695.1
Expression Level Scale
Very high
log, scaled to maximum expression level
Tissue
Expression Level
Larval Central Nervous System
3080.95
Larval Midgut
5226.1
Larval Hindgut
5141.1
Larval Malpighian Tubules
6411.1
Larval Fat Body
8869.3
Larval Salivary Gland
9397
Larval Trachea
7494.95
Larval Carcass
5633.7
Adult Head
5215.4
Adult Eye
8606.425
Adult Brain
3475.1
Adult Thoracic-Abdominal Ganglion
4947.2
Adult Crop
7390.3
Adult Midgut
6065.4
Adult Hindgut
5834.5
Adult Malpighian Tubules
7264.7
Adult Fat Body
6351.8
Adult Salivary Gland
14913.8
Adult Heart
8450.4
Adult VirginFemale Spermatheca
6446.4
Adult InseminatedFemale Spermatheca
6648.4
Adult Ovary
4338.5
Adult Testis
1713.8
Adult Male Accessory Gland
11197.2
Adult Carcass
6695.1
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Moderate expression
Tissue
Expression Level
Larval Central Nervous System
(3080.95)
Larval Midgut
(5226.1)
Larval Hindgut
(5141.1)
Larval Malpighian Tubules
(6411.1)
Larval Fat Body
(8869.3)
Larval Salivary Gland
(9397)
Larval Trachea
(7494.95)
Larval Carcass
(5633.7)
Adult Head
(5215.4)
Adult Eye
(8606.425)
Adult Brain
(3475.1)
Adult Thoracic-Abdominal Ganglion
(4947.2)
Adult Crop
(7390.3)
Adult Midgut
(6065.4)
Adult Hindgut
(5834.5)
Adult Malpighian Tubules
(7264.7)
Adult Fat Body
(6351.8)
Adult Salivary Gland
(14913.8)
Adult Heart
(8450.4)
Adult VirginFemale Spermatheca
(6446.4)
Adult InseminatedFemale Spermatheca
(6648.4)
Adult Ovary
(4338.5)
Adult Testis
(1713.8)
Adult Male Accessory Gland
(11197.2)
Adult Carcass
(6695.1)
Expression Level Scale
None
Low
Moderate
High
log, scaled to High level expression
Tissue
Expression Level
Larval Central Nervous System
(3080.95)
Larval Midgut
(5226.1)
Larval Hindgut
(5141.1)
Larval Malpighian Tubules
(6411.1)
Larval Fat Body
(8869.3)
Larval Salivary Gland
(9397)
Larval Trachea
(7494.95)
Larval Carcass
(5633.7)
Adult Head
(5215.4)
Adult Eye
(8606.425)
Adult Brain
(3475.1)
Adult Thoracic-Abdominal Ganglion
(4947.2)
Adult Crop
(7390.3)
Adult Midgut
(6065.4)
Adult Hindgut
(5834.5)
Adult Malpighian Tubules
(7264.7)
Adult Fat Body
(6351.8)
Adult Salivary Gland
(14913.8)
Adult Heart
(8450.4)
Adult VirginFemale Spermatheca
(6446.4)
Adult InseminatedFemale Spermatheca
(6648.4)
Adult Ovary
(4338.5)
Adult Testis
1713.8
Adult Male Accessory Gland
(11197.2)
Adult Carcass
(6695.1)
Expression Level Scale
None
Low
Moderate
High
Very high
log, scaled to Very high expression
Tissue
Expression Level
Larval Central Nervous System
3080.95
Larval Midgut
5226.1
Larval Hindgut
5141.1
Larval Malpighian Tubules
6411.1
Larval Fat Body
8869.3
Larval Salivary Gland
9397
Larval Trachea
7494.95
Larval Carcass
5633.7
Adult Head
5215.4
Adult Eye
8606.425
Adult Brain
3475.1
Adult Thoracic-Abdominal Ganglion
4947.2
Adult Crop
7390.3
Adult Midgut
6065.4
Adult Hindgut
5834.5
Adult Malpighian Tubules
7264.7
Adult Fat Body
6351.8
Adult Salivary Gland
14913.8
Adult Heart
8450.4
Adult VirginFemale Spermatheca
6446.4
Adult InseminatedFemale Spermatheca
6648.4
Adult Ovary
4338.5
Adult Testis
1713.8
Adult Male Accessory Gland
11197.2
Adult Carcass
6695.1
Expression Level Scale
None
Low
Moderate
High
Very high
Heatmap
Tissue
Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass
FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level
Tissue
Adult Expression Level
NA
Head
5215.4
NA
Eye
8606.425
NA
Brain
3475.1
3080.95
Central Nervous System
NA
NA
Thoracic-Abdominal Ganglion
4947.2
NA
Crop
7390.3
5226.1
Midgut
6065.4
5141.1
Hindgut
5834.5
6411.1
Malpighian Tubules
7264.7
8869.3
Fat Body
6351.8
9397
Salivary Gland
14913.8
NA
Heart
8450.4
7494.95
Trachea
NA
NA
VirginFemale Spermatheca
6446.4
NA
InseminatedFemale Spermatheca
6648.4
NA
Ovary
4338.5
NA
Testis
1713.8
NA
Male Accessory Gland
11197.2
5633.7
Carcass
6695.1
modENCODE Anatomy RNA-Seq
Linear, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
315
central nervous system, larvae L3
306
central nervous system, pupae P8
201
head, virgin 1-day female
340
head, virgin 4-day female
323
head, virgin 20-day female
384
head, mated 1-day female
210
head, mated 4-day female
317
head, mated 20-day female
296
head, mated 1-day male
224
head, mated 4-day male
241
head, mated 20-day male
316
salivary gland, larvae L3 wandering
946
salivary gland, white prepupae
1045
digestive system, larvae L3 wandering
334
digestive system, 1-day adult
293
digestive system, 4-day adult
266
digestive system, 20-day adult
196
fat body, larvae L3 wandering
119
fat body, white prepupae
222
fat body, pupae P8
240
carcass, larvae L3 wandering
452
carcass, 1-day adult
208
carcass, 4-day adult
265
carcass, 20-day adult
245
ovary, virgin 4-day female
874
ovary, mated 4-day female
648
testis, mated 4-day male
149
accessory gland, mated 4-day male
352
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Linear, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(315)
central nervous system, larvae L3
(306)
central nervous system, pupae P8
(201)
head, virgin 1-day female
(340)
head, virgin 4-day female
(323)
head, virgin 20-day female
(384)
head, mated 1-day female
(210)
head, mated 4-day female
(317)
head, mated 20-day female
(296)
head, mated 1-day male
(224)
head, mated 4-day male
(241)
head, mated 20-day male
(316)
salivary gland, larvae L3 wandering
(946)
salivary gland, white prepupae
(1045)
digestive system, larvae L3 wandering
(334)
digestive system, 1-day adult
(293)
digestive system, 4-day adult
(266)
digestive system, 20-day adult
(196)
fat body, larvae L3 wandering
(119)
fat body, white prepupae
(222)
fat body, pupae P8
(240)
carcass, larvae L3 wandering
(452)
carcass, 1-day adult
(208)
carcass, 4-day adult
(265)
carcass, 20-day adult
(245)
ovary, virgin 4-day female
(874)
ovary, mated 4-day female
(648)
testis, mated 4-day male
(149)
accessory gland, mated 4-day male
(352)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(315)
central nervous system, larvae L3
(306)
central nervous system, pupae P8
(201)
head, virgin 1-day female
(340)
head, virgin 4-day female
(323)
head, virgin 20-day female
(384)
head, mated 1-day female
(210)
head, mated 4-day female
(317)
head, mated 20-day female
(296)
head, mated 1-day male
(224)
head, mated 4-day male
(241)
head, mated 20-day male
(316)
salivary gland, larvae L3 wandering
(946)
salivary gland, white prepupae
(1045)
digestive system, larvae L3 wandering
(334)
digestive system, 1-day adult
(293)
digestive system, 4-day adult
(266)
digestive system, 20-day adult
(196)
fat body, larvae L3 wandering
(119)
fat body, white prepupae
(222)
fat body, pupae P8
(240)
carcass, larvae L3 wandering
(452)
carcass, 1-day adult
(208)
carcass, 4-day adult
(265)
carcass, 20-day adult
(245)
ovary, virgin 4-day female
(874)
ovary, mated 4-day female
(648)
testis, mated 4-day male
(149)
accessory gland, mated 4-day male
(352)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
315
central nervous system, larvae L3
306
central nervous system, pupae P8
201
head, virgin 1-day female
340
head, virgin 4-day female
323
head, virgin 20-day female
384
head, mated 1-day female
210
head, mated 4-day female
317
head, mated 20-day female
296
head, mated 1-day male
224
head, mated 4-day male
241
head, mated 20-day male
316
salivary gland, larvae L3 wandering
946
salivary gland, white prepupae
1045
digestive system, larvae L3 wandering
334
digestive system, 1-day adult
293
digestive system, 4-day adult
266
digestive system, 20-day adult
196
fat body, larvae L3 wandering
119
fat body, white prepupae
222
fat body, pupae P8
240
carcass, larvae L3 wandering
452
carcass, 1-day adult
208
carcass, 4-day adult
265
carcass, 20-day adult
245
ovary, virgin 4-day female
874
ovary, mated 4-day female
648
testis, mated 4-day male
149
accessory gland, mated 4-day male
352
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Tissue
Expression Level
imaginal disc, larvae L3 wandering
315
central nervous system, larvae L3
306
central nervous system, pupae P8
201
head, virgin 1-day female
340
head, virgin 4-day female
323
head, virgin 20-day female
384
head, mated 1-day female
210
head, mated 4-day female
317
head, mated 20-day female
296
head, mated 1-day male
224
head, mated 4-day male
241
head, mated 20-day male
316
salivary gland, larvae L3 wandering
946
salivary gland, white prepupae
1045
digestive system, larvae L3 wandering
334
digestive system, 1-day adult
293
digestive system, 4-day adult
266
digestive system, 20-day adult
196
fat body, larvae L3 wandering
119
fat body, white prepupae
222
fat body, pupae P8
240
carcass, larvae L3 wandering
452
carcass, 1-day adult
208
carcass, 4-day adult
265
carcass, 20-day adult
245
ovary, virgin 4-day female
874
ovary, mated 4-day female
648
testis, mated 4-day male
149
accessory gland, mated 4-day male
352
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to Moderate expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(315)
central nervous system, larvae L3
(306)
central nervous system, pupae P8
(201)
head, virgin 1-day female
(340)
head, virgin 4-day female
(323)
head, virgin 20-day female
(384)
head, mated 1-day female
(210)
head, mated 4-day female
(317)
head, mated 20-day female
(296)
head, mated 1-day male
(224)
head, mated 4-day male
(241)
head, mated 20-day male
(316)
salivary gland, larvae L3 wandering
(946)
salivary gland, white prepupae
(1045)
digestive system, larvae L3 wandering
(334)
digestive system, 1-day adult
(293)
digestive system, 4-day adult
(266)
digestive system, 20-day adult
(196)
fat body, larvae L3 wandering
(119)
fat body, white prepupae
(222)
fat body, pupae P8
(240)
carcass, larvae L3 wandering
(452)
carcass, 1-day adult
(208)
carcass, 4-day adult
(265)
carcass, 20-day adult
(245)
ovary, virgin 4-day female
(874)
ovary, mated 4-day female
(648)
testis, mated 4-day male
(149)
accessory gland, mated 4-day male
(352)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
(315)
central nervous system, larvae L3
(306)
central nervous system, pupae P8
(201)
head, virgin 1-day female
(340)
head, virgin 4-day female
(323)
head, virgin 20-day female
(384)
head, mated 1-day female
(210)
head, mated 4-day female
(317)
head, mated 20-day female
(296)
head, mated 1-day male
(224)
head, mated 4-day male
(241)
head, mated 20-day male
(316)
salivary gland, larvae L3 wandering
(946)
salivary gland, white prepupae
(1045)
digestive system, larvae L3 wandering
(334)
digestive system, 1-day adult
(293)
digestive system, 4-day adult
(266)
digestive system, 20-day adult
(196)
fat body, larvae L3 wandering
119
fat body, white prepupae
(222)
fat body, pupae P8
(240)
carcass, larvae L3 wandering
(452)
carcass, 1-day adult
(208)
carcass, 4-day adult
(265)
carcass, 20-day adult
(245)
ovary, virgin 4-day female
(874)
ovary, mated 4-day female
(648)
testis, mated 4-day male
(149)
accessory gland, mated 4-day male
(352)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Tissue
Expression Level
imaginal disc, larvae L3 wandering
315
central nervous system, larvae L3
306
central nervous system, pupae P8
201
head, virgin 1-day female
340
head, virgin 4-day female
323
head, virgin 20-day female
384
head, mated 1-day female
210
head, mated 4-day female
317
head, mated 20-day female
296
head, mated 1-day male
224
head, mated 4-day male
241
head, mated 20-day male
316
salivary gland, larvae L3 wandering
946
salivary gland, white prepupae
1045
digestive system, larvae L3 wandering
334
digestive system, 1-day adult
293
digestive system, 4-day adult
266
digestive system, 20-day adult
196
fat body, larvae L3 wandering
119
fat body, white prepupae
222
fat body, pupae P8
240
carcass, larvae L3 wandering
452
carcass, 1-day adult
208
carcass, 4-day adult
265
carcass, 20-day adult
245
ovary, virgin 4-day female
874
ovary, mated 4-day female
648
testis, mated 4-day male
149
accessory gland, mated 4-day male
352
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Tissue
Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male
modENCODE Development RNA-Seq
Linear, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
1277
embryo 02-04hr
2144
embryo 04-06hr
2587
embryo 06-08hr
2053
embryo 08-10hr
2381
embryo 10-12hr
2276
embryo 12-14hr
2515
embryo 14-16hr
1006
embryo 16-18hr
1439
embryo 18-20hr
1107
embryo 20-22hr
1388
embryo 22-24hr
1909
larva L1
1356
larva L2
1774
larva L3 12hr old
1392
larva L3 puffstage 1-2
1178
larva L3 puffstage 3-6
1105
larva L3 puffstage 7-9
1408
white prepupae new
1102
white prepupae 12hr
962
white prepupae 24hr
831
pupae 2d postWPP
809
pupae 3d postWPP
727
pupae 4d postWPP
444
adult male 01day
548
adult male 05day
452
adult male 30day
346
adult female 01day
793
adult female 05day
1729
adult female 30day
1605
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Linear, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(1277)
embryo 02-04hr
(2144)
embryo 04-06hr
(2587)
embryo 06-08hr
(2053)
embryo 08-10hr
(2381)
embryo 10-12hr
(2276)
embryo 12-14hr
(2515)
embryo 14-16hr
(1006)
embryo 16-18hr
(1439)
embryo 18-20hr
(1107)
embryo 20-22hr
(1388)
embryo 22-24hr
(1909)
larva L1
(1356)
larva L2
(1774)
larva L3 12hr old
(1392)
larva L3 puffstage 1-2
(1178)
larva L3 puffstage 3-6
(1105)
larva L3 puffstage 7-9
(1408)
white prepupae new
(1102)
white prepupae 12hr
(962)
white prepupae 24hr
(831)
pupae 2d postWPP
(809)
pupae 3d postWPP
(727)
pupae 4d postWPP
(444)
adult male 01day
(548)
adult male 05day
(452)
adult male 30day
(346)
adult female 01day
(793)
adult female 05day
(1729)
adult female 30day
(1605)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
(1277)
embryo 02-04hr
(2144)
embryo 04-06hr
(2587)
embryo 06-08hr
(2053)
embryo 08-10hr
(2381)
embryo 10-12hr
(2276)
embryo 12-14hr
(2515)
embryo 14-16hr
(1006)
embryo 16-18hr
(1439)
embryo 18-20hr
(1107)
embryo 20-22hr
(1388)
embryo 22-24hr
(1909)
larva L1
(1356)
larva L2
(1774)
larva L3 12hr old
(1392)
larva L3 puffstage 1-2
(1178)
larva L3 puffstage 3-6
(1105)
larva L3 puffstage 7-9
(1408)
white prepupae new
(1102)
white prepupae 12hr
(962)
white prepupae 24hr
(831)
pupae 2d postWPP
(809)
pupae 3d postWPP
(727)
pupae 4d postWPP
(444)
adult male 01day
(548)
adult male 05day
(452)
adult male 30day
(346)
adult female 01day
(793)
adult female 05day
(1729)
adult female 30day
(1605)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
1277
embryo 02-04hr
2144
embryo 04-06hr
2587
embryo 06-08hr
2053
embryo 08-10hr
2381
embryo 10-12hr
2276
embryo 12-14hr
2515
embryo 14-16hr
1006
embryo 16-18hr
1439
embryo 18-20hr
1107
embryo 20-22hr
1388
embryo 22-24hr
1909
larva L1
1356
larva L2
1774
larva L3 12hr old
1392
larva L3 puffstage 1-2
1178
larva L3 puffstage 3-6
1105
larva L3 puffstage 7-9
1408
white prepupae new
1102
white prepupae 12hr
962
white prepupae 24hr
831
pupae 2d postWPP
809
pupae 3d postWPP
727
pupae 4d postWPP
444
adult male 01day
548
adult male 05day
452
adult male 30day
346
adult female 01day
793
adult female 05day
1729
adult female 30day
1605
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Developmental Stage
Expression Level
embryo 00-02hr
1277
embryo 02-04hr
2144
embryo 04-06hr
2587
embryo 06-08hr
2053
embryo 08-10hr
2381
embryo 10-12hr
2276
embryo 12-14hr
2515
embryo 14-16hr
1006
embryo 16-18hr
1439
embryo 18-20hr
1107
embryo 20-22hr
1388
embryo 22-24hr
1909
larva L1
1356
larva L2
1774
larva L3 12hr old
1392
larva L3 puffstage 1-2
1178
larva L3 puffstage 3-6
1105
larva L3 puffstage 7-9
1408
white prepupae new
1102
white prepupae 12hr
962
white prepupae 24hr
831
pupae 2d postWPP
809
pupae 3d postWPP
727
pupae 4d postWPP
444
adult male 01day
548
adult male 05day
452
adult male 30day
346
adult female 01day
793
adult female 05day
1729
adult female 30day
1605
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to Moderate expression
Developmental Stage
Expression Level
embryo 00-02hr
(1277)
embryo 02-04hr
(2144)
embryo 04-06hr
(2587)
embryo 06-08hr
(2053)
embryo 08-10hr
(2381)
embryo 10-12hr
(2276)
embryo 12-14hr
(2515)
embryo 14-16hr
(1006)
embryo 16-18hr
(1439)
embryo 18-20hr
(1107)
embryo 20-22hr
(1388)
embryo 22-24hr
(1909)
larva L1
(1356)
larva L2
(1774)
larva L3 12hr old
(1392)
larva L3 puffstage 1-2
(1178)
larva L3 puffstage 3-6
(1105)
larva L3 puffstage 7-9
(1408)
white prepupae new
(1102)
white prepupae 12hr
(962)
white prepupae 24hr
(831)
pupae 2d postWPP
(809)
pupae 3d postWPP
(727)
pupae 4d postWPP
(444)
adult male 01day
(548)
adult male 05day
(452)
adult male 30day
(346)
adult female 01day
(793)
adult female 05day
(1729)
adult female 30day
(1605)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Developmental Stage
Expression Level
embryo 00-02hr
(1277)
embryo 02-04hr
(2144)
embryo 04-06hr
(2587)
embryo 06-08hr
(2053)
embryo 08-10hr
(2381)
embryo 10-12hr
(2276)
embryo 12-14hr
(2515)
embryo 14-16hr
(1006)
embryo 16-18hr
(1439)
embryo 18-20hr
(1107)
embryo 20-22hr
(1388)
embryo 22-24hr
(1909)
larva L1
(1356)
larva L2
(1774)
larva L3 12hr old
(1392)
larva L3 puffstage 1-2
(1178)
larva L3 puffstage 3-6
(1105)
larva L3 puffstage 7-9
(1408)
white prepupae new
(1102)
white prepupae 12hr
(962)
white prepupae 24hr
(831)
pupae 2d postWPP
(809)
pupae 3d postWPP
(727)
pupae 4d postWPP
(444)
adult male 01day
(548)
adult male 05day
(452)
adult male 30day
(346)
adult female 01day
(793)
adult female 05day
(1729)
adult female 30day
(1605)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Developmental Stage
Expression Level
embryo 00-02hr
1277
embryo 02-04hr
2144
embryo 04-06hr
2587
embryo 06-08hr
2053
embryo 08-10hr
2381
embryo 10-12hr
2276
embryo 12-14hr
2515
embryo 14-16hr
1006
embryo 16-18hr
1439
embryo 18-20hr
1107
embryo 20-22hr
1388
embryo 22-24hr
1909
larva L1
1356
larva L2
1774
larva L3 12hr old
1392
larva L3 puffstage 1-2
1178
larva L3 puffstage 3-6
1105
larva L3 puffstage 7-9
1408
white prepupae new
1102
white prepupae 12hr
962
white prepupae 24hr
831
pupae 2d postWPP
809
pupae 3d postWPP
727
pupae 4d postWPP
444
adult male 01day
548
adult male 05day
452
adult male 30day
346
adult female 01day
793
adult female 05day
1729
adult female 30day
1605
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Developmental Stage
Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day
modENCODE Cell Lines RNA-Seq
Linear, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
740
Schneider line 2 Sg4
372
embryonic 1182-4H
408
embryonic GM2
1579
embryonic Kc167
926
embryonic S1
261
embryonic S3
940
leg disc CME L1
327
wing disc CME-W2
1283
wing disc ML-DmD8
1475
wing disc ML-DmD9
283
wing disc ML-DmD16-c3
539
wing disc ML-DmD21
337
wing disc ML-DmD32
241
haltere disc ML-DmD17-c3
797
eye-antennal disc ML-DmD11
629
antennal disc ML-DmD20-c5
927
mixed discs ML-DmD4-c1
896
CNS ML-DmBG1-c1
383
CNS ML-DmBG2-c2
1057
tumorous blood cells mbn2
872
ovary fGS/OSS
262
ovary OSC
398
ovary OSS
214
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Linear, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(740)
Schneider line 2 Sg4
(372)
embryonic 1182-4H
(408)
embryonic GM2
(1579)
embryonic Kc167
(926)
embryonic S1
(261)
embryonic S3
(940)
leg disc CME L1
(327)
wing disc CME-W2
(1283)
wing disc ML-DmD8
(1475)
wing disc ML-DmD9
(283)
wing disc ML-DmD16-c3
(539)
wing disc ML-DmD21
(337)
wing disc ML-DmD32
(241)
haltere disc ML-DmD17-c3
(797)
eye-antennal disc ML-DmD11
(629)
antennal disc ML-DmD20-c5
(927)
mixed discs ML-DmD4-c1
(896)
CNS ML-DmBG1-c1
(383)
CNS ML-DmBG2-c2
(1057)
tumorous blood cells mbn2
(872)
ovary fGS/OSS
(262)
ovary OSC
(398)
ovary OSS
(214)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
(740)
Schneider line 2 Sg4
(372)
embryonic 1182-4H
(408)
embryonic GM2
(1579)
embryonic Kc167
(926)
embryonic S1
(261)
embryonic S3
(940)
leg disc CME L1
(327)
wing disc CME-W2
(1283)
wing disc ML-DmD8
(1475)
wing disc ML-DmD9
(283)
wing disc ML-DmD16-c3
(539)
wing disc ML-DmD21
(337)
wing disc ML-DmD32
(241)
haltere disc ML-DmD17-c3
(797)
eye-antennal disc ML-DmD11
(629)
antennal disc ML-DmD20-c5
(927)
mixed discs ML-DmD4-c1
(896)
CNS ML-DmBG1-c1
(383)
CNS ML-DmBG2-c2
(1057)
tumorous blood cells mbn2
(872)
ovary fGS/OSS
(262)
ovary OSC
(398)
ovary OSS
(214)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
740
Schneider line 2 Sg4
372
embryonic 1182-4H
408
embryonic GM2
1579
embryonic Kc167
926
embryonic S1
261
embryonic S3
940
leg disc CME L1
327
wing disc CME-W2
1283
wing disc ML-DmD8
1475
wing disc ML-DmD9
283
wing disc ML-DmD16-c3
539
wing disc ML-DmD21
337
wing disc ML-DmD32
241
haltere disc ML-DmD17-c3
797
eye-antennal disc ML-DmD11
629
antennal disc ML-DmD20-c5
927
mixed discs ML-DmD4-c1
896
CNS ML-DmBG1-c1
383
CNS ML-DmBG2-c2
1057
tumorous blood cells mbn2
872
ovary fGS/OSS
262
ovary OSC
398
ovary OSS
214
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Cell Line
Expression Level
Schneider line 2 S2R+
740
Schneider line 2 Sg4
372
embryonic 1182-4H
408
embryonic GM2
1579
embryonic Kc167
926
embryonic S1
261
embryonic S3
940
leg disc CME L1
327
wing disc CME-W2
1283
wing disc ML-DmD8
1475
wing disc ML-DmD9
283
wing disc ML-DmD16-c3
539
wing disc ML-DmD21
337
wing disc ML-DmD32
241
haltere disc ML-DmD17-c3
797
eye-antennal disc ML-DmD11
629
antennal disc ML-DmD20-c5
927
mixed discs ML-DmD4-c1
896
CNS ML-DmBG1-c1
383
CNS ML-DmBG2-c2
1057
tumorous blood cells mbn2
872
ovary fGS/OSS
262
ovary OSC
398
ovary OSS
214
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to Moderate expression
Cell Line
Expression Level
Schneider line 2 S2R+
(740)
Schneider line 2 Sg4
(372)
embryonic 1182-4H
(408)
embryonic GM2
(1579)
embryonic Kc167
(926)
embryonic S1
(261)
embryonic S3
(940)
leg disc CME L1
(327)
wing disc CME-W2
(1283)
wing disc ML-DmD8
(1475)
wing disc ML-DmD9
(283)
wing disc ML-DmD16-c3
(539)
wing disc ML-DmD21
(337)
wing disc ML-DmD32
(241)
haltere disc ML-DmD17-c3
(797)
eye-antennal disc ML-DmD11
(629)
antennal disc ML-DmD20-c5
(927)
mixed discs ML-DmD4-c1
(896)
CNS ML-DmBG1-c1
(383)
CNS ML-DmBG2-c2
(1057)
tumorous blood cells mbn2
(872)
ovary fGS/OSS
(262)
ovary OSC
(398)
ovary OSS
(214)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Cell Line
Expression Level
Schneider line 2 S2R+
(740)
Schneider line 2 Sg4
(372)
embryonic 1182-4H
(408)
embryonic GM2
(1579)
embryonic Kc167
(926)
embryonic S1
(261)
embryonic S3
(940)
leg disc CME L1
(327)
wing disc CME-W2
(1283)
wing disc ML-DmD8
(1475)
wing disc ML-DmD9
(283)
wing disc ML-DmD16-c3
(539)
wing disc ML-DmD21
(337)
wing disc ML-DmD32
(241)
haltere disc ML-DmD17-c3
(797)
eye-antennal disc ML-DmD11
(629)
antennal disc ML-DmD20-c5
(927)
mixed discs ML-DmD4-c1
(896)
CNS ML-DmBG1-c1
(383)
CNS ML-DmBG2-c2
(1057)
tumorous blood cells mbn2
(872)
ovary fGS/OSS
(262)
ovary OSC
(398)
ovary OSS
(214)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Cell Line
Expression Level
Schneider line 2 S2R+
740
Schneider line 2 Sg4
372
embryonic 1182-4H
408
embryonic GM2
1579
embryonic Kc167
926
embryonic S1
261
embryonic S3
940
leg disc CME L1
327
wing disc CME-W2
1283
wing disc ML-DmD8
1475
wing disc ML-DmD9
283
wing disc ML-DmD16-c3
539
wing disc ML-DmD21
337
wing disc ML-DmD32
241
haltere disc ML-DmD17-c3
797
eye-antennal disc ML-DmD11
629
antennal disc ML-DmD20-c5
927
mixed discs ML-DmD4-c1
896
CNS ML-DmBG1-c1
383
CNS ML-DmBG2-c2
1057
tumorous blood cells mbn2
872
ovary fGS/OSS
262
ovary OSC
398
ovary OSS
214
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Cell Line
Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS
modENCODE Treatments RNA-Seq
Linear, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
140
cold shock, 4-day adult
115
heat shock, 4-day adult
585
Cadmium 50 mM 6 hrs, larvae L3
538
Cadmium 50 mM 12 hrs, larvae L3
1013
Cadmium 50 mM 48 hrs, 4-day adult
539
Cadmium 100 mM 48 hrs, 4-day adult
556
Copper 0.5 mM 12 hrs, larvae L3
235
Copper 15 mM 48 hrs, 4-day adult
402
Zinc 5 mM 12 hrs, larvae L3
179
Zinc 4.5 mM 48 hrs, 4-day adult
352
Ethanol 2.5% 3 hrs, larvae L3
559
Ethanol 5% 3 hrs, larvae L3
1047
Ethanol 10% 3 hrs, larvae L3
563
Caffeine 1.5 mg/ml 4 hrs, larvae L3
932
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
562
Caffeine 25 mg/ml 48 hrs, 4-day adult
157
Paraquat 5 mM 48 hrs, 4-day adult
575
Paraquat 10 mM 48 hrs, 4-day adult
522
Rotenone 2 μg 12 hrs, larvae L3
954
Rotenone 8 μg 12 hrs, larvae L3
328
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Linear, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
(140)
cold shock, 4-day adult
(115)
heat shock, 4-day adult
(585)
Cadmium 50 mM 6 hrs, larvae L3
(538)
Cadmium 50 mM 12 hrs, larvae L3
(1013)
Cadmium 50 mM 48 hrs, 4-day adult
(539)
Cadmium 100 mM 48 hrs, 4-day adult
(556)
Copper 0.5 mM 12 hrs, larvae L3
(235)
Copper 15 mM 48 hrs, 4-day adult
(402)
Zinc 5 mM 12 hrs, larvae L3
(179)
Zinc 4.5 mM 48 hrs, 4-day adult
(352)
Ethanol 2.5% 3 hrs, larvae L3
(559)
Ethanol 5% 3 hrs, larvae L3
(1047)
Ethanol 10% 3 hrs, larvae L3
(563)
Caffeine 1.5 mg/ml 4 hrs, larvae L3
(932)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(157)
Paraquat 5 mM 48 hrs, 4-day adult
(575)
Paraquat 10 mM 48 hrs, 4-day adult
(522)
Rotenone 2 μg 12 hrs, larvae L3
(954)
Rotenone 8 μg 12 hrs, larvae L3
(328)
Expression Level Scale
Very low
Low
Moderate
Moderately high
Linear, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
(140)
cold shock, 4-day adult
(115)
heat shock, 4-day adult
(585)
Cadmium 50 mM 6 hrs, larvae L3
(538)
Cadmium 50 mM 12 hrs, larvae L3
(1013)
Cadmium 50 mM 48 hrs, 4-day adult
(539)
Cadmium 100 mM 48 hrs, 4-day adult
(556)
Copper 0.5 mM 12 hrs, larvae L3
(235)
Copper 15 mM 48 hrs, 4-day adult
(402)
Zinc 5 mM 12 hrs, larvae L3
(179)
Zinc 4.5 mM 48 hrs, 4-day adult
(352)
Ethanol 2.5% 3 hrs, larvae L3
(559)
Ethanol 5% 3 hrs, larvae L3
(1047)
Ethanol 10% 3 hrs, larvae L3
(563)
Caffeine 1.5 mg/ml 4 hrs, larvae L3
(932)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(157)
Paraquat 5 mM 48 hrs, 4-day adult
(575)
Paraquat 10 mM 48 hrs, 4-day adult
(522)
Rotenone 2 μg 12 hrs, larvae L3
(954)
Rotenone 8 μg 12 hrs, larvae L3
(328)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Linear, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
140
cold shock, 4-day adult
115
heat shock, 4-day adult
585
Cadmium 50 mM 6 hrs, larvae L3
538
Cadmium 50 mM 12 hrs, larvae L3
1013
Cadmium 50 mM 48 hrs, 4-day adult
539
Cadmium 100 mM 48 hrs, 4-day adult
556
Copper 0.5 mM 12 hrs, larvae L3
235
Copper 15 mM 48 hrs, 4-day adult
402
Zinc 5 mM 12 hrs, larvae L3
179
Zinc 4.5 mM 48 hrs, 4-day adult
352
Ethanol 2.5% 3 hrs, larvae L3
559
Ethanol 5% 3 hrs, larvae L3
1047
Ethanol 10% 3 hrs, larvae L3
563
Caffeine 1.5 mg/ml 4 hrs, larvae L3
932
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
562
Caffeine 25 mg/ml 48 hrs, 4-day adult
157
Paraquat 5 mM 48 hrs, 4-day adult
575
Paraquat 10 mM 48 hrs, 4-day adult
522
Rotenone 2 μg 12 hrs, larvae L3
954
Rotenone 8 μg 12 hrs, larvae L3
328
Expression Level Scale
Extremely high
log, scaled to maximum expression level
Treatment
Expression Level
extended cold, 4-day adult
140
cold shock, 4-day adult
115
heat shock, 4-day adult
585
Cadmium 50 mM 6 hrs, larvae L3
538
Cadmium 50 mM 12 hrs, larvae L3
1013
Cadmium 50 mM 48 hrs, 4-day adult
539
Cadmium 100 mM 48 hrs, 4-day adult
556
Copper 0.5 mM 12 hrs, larvae L3
235
Copper 15 mM 48 hrs, 4-day adult
402
Zinc 5 mM 12 hrs, larvae L3
179
Zinc 4.5 mM 48 hrs, 4-day adult
352
Ethanol 2.5% 3 hrs, larvae L3
559
Ethanol 5% 3 hrs, larvae L3
1047
Ethanol 10% 3 hrs, larvae L3
563
Caffeine 1.5 mg/ml 4 hrs, larvae L3
932
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
562
Caffeine 25 mg/ml 48 hrs, 4-day adult
157
Paraquat 5 mM 48 hrs, 4-day adult
575
Paraquat 10 mM 48 hrs, 4-day adult
522
Rotenone 2 μg 12 hrs, larvae L3
954
Rotenone 8 μg 12 hrs, larvae L3
328
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
log, scaled to Moderate expression
Treatment
Expression Level
extended cold, 4-day adult
(140)
cold shock, 4-day adult
(115)
heat shock, 4-day adult
(585)
Cadmium 50 mM 6 hrs, larvae L3
(538)
Cadmium 50 mM 12 hrs, larvae L3
(1013)
Cadmium 50 mM 48 hrs, 4-day adult
(539)
Cadmium 100 mM 48 hrs, 4-day adult
(556)
Copper 0.5 mM 12 hrs, larvae L3
(235)
Copper 15 mM 48 hrs, 4-day adult
(402)
Zinc 5 mM 12 hrs, larvae L3
(179)
Zinc 4.5 mM 48 hrs, 4-day adult
(352)
Ethanol 2.5% 3 hrs, larvae L3
(559)
Ethanol 5% 3 hrs, larvae L3
(1047)
Ethanol 10% 3 hrs, larvae L3
(563)
Caffeine 1.5 mg/ml 4 hrs, larvae L3
(932)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(157)
Paraquat 5 mM 48 hrs, 4-day adult
(575)
Paraquat 10 mM 48 hrs, 4-day adult
(522)
Rotenone 2 μg 12 hrs, larvae L3
(954)
Rotenone 8 μg 12 hrs, larvae L3
(328)
Expression Level Scale
Very low
Low
Moderate
Moderately high
log, scaled to High expression
Treatment
Expression Level
extended cold, 4-day adult
140
cold shock, 4-day adult
115
heat shock, 4-day adult
(585)
Cadmium 50 mM 6 hrs, larvae L3
(538)
Cadmium 50 mM 12 hrs, larvae L3
(1013)
Cadmium 50 mM 48 hrs, 4-day adult
(539)
Cadmium 100 mM 48 hrs, 4-day adult
(556)
Copper 0.5 mM 12 hrs, larvae L3
(235)
Copper 15 mM 48 hrs, 4-day adult
(402)
Zinc 5 mM 12 hrs, larvae L3
(179)
Zinc 4.5 mM 48 hrs, 4-day adult
(352)
Ethanol 2.5% 3 hrs, larvae L3
(559)
Ethanol 5% 3 hrs, larvae L3
(1047)
Ethanol 10% 3 hrs, larvae L3
(563)
Caffeine 1.5 mg/ml 4 hrs, larvae L3
(932)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
(562)
Caffeine 25 mg/ml 48 hrs, 4-day adult
(157)
Paraquat 5 mM 48 hrs, 4-day adult
(575)
Paraquat 10 mM 48 hrs, 4-day adult
(522)
Rotenone 2 μg 12 hrs, larvae L3
(954)
Rotenone 8 μg 12 hrs, larvae L3
(328)
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
log, scaled to Extremely high expression
Treatment
Expression Level
extended cold, 4-day adult
140
cold shock, 4-day adult
115
heat shock, 4-day adult
585
Cadmium 50 mM 6 hrs, larvae L3
538
Cadmium 50 mM 12 hrs, larvae L3
1013
Cadmium 50 mM 48 hrs, 4-day adult
539
Cadmium 100 mM 48 hrs, 4-day adult
556
Copper 0.5 mM 12 hrs, larvae L3
235
Copper 15 mM 48 hrs, 4-day adult
402
Zinc 5 mM 12 hrs, larvae L3
179
Zinc 4.5 mM 48 hrs, 4-day adult
352
Ethanol 2.5% 3 hrs, larvae L3
559
Ethanol 5% 3 hrs, larvae L3
1047
Ethanol 10% 3 hrs, larvae L3
563
Caffeine 1.5 mg/ml 4 hrs, larvae L3
932
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
562
Caffeine 25 mg/ml 48 hrs, 4-day adult
157
Paraquat 5 mM 48 hrs, 4-day adult
575
Paraquat 10 mM 48 hrs, 4-day adult
522
Rotenone 2 μg 12 hrs, larvae L3
954
Rotenone 8 μg 12 hrs, larvae L3
328
Expression Level Scale
Very low
Low
Moderate
Moderately high
High
Very high
Extremely high
Heatmap
Treatment
Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
A cluster of genes with similar mRNA expression dynamics across development.
External Data & Images
Linkouts
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
Alleles & Phenotypes Summary of Allele Phenotypes Classical Alleles ( 1 ) For All Classical Alleles Show Allele of
RpS3A Class Mutagen Stocks Known lesion RpS3A57g 1 Yes
Alleles Carried on Transgenic Constructs
( 6 ) For All Alleles Carried on Transgenic
Constructs Show Aneuploid Aberrations Disrupted in Not disrupted in Not duplicated in Transgenic Constructs & Insertions
Transgenic Constructs Insertions Gene Ontology: Function, Process
& Cellular Component ( 7 unique terms ) Terms Based on
Experimental Evidence ( 2 terms ) Molecular Function ( 0 terms) Biological Process inferred from mutant phenotype
inferred from mutant phenotype
Cellular Component ( 0 terms) Terms Based on Predictions or
Assertions ( 5 terms ) Molecular Function inferred from sequence or structural similarity with
human RPS3A non-traceable author statement
Biological Process non-traceable author statement
inferred from sequence or structural similarity with
human RPS3A non-traceable author statement
Cellular Component non-traceable author statement
inferred from sequence or structural similarity with
human RPS3A non-traceable author statement
Sequence Ontology: Class of Gene Interactions & Pathways
Summary of Physical Interactions
protein-protein anti tag coimmunoprecipitation, peptide massfingerprinting
Summary of Genetic Interactions Interacts with Please look at the allele data
for full details of the genetic interactions
External Data Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
Orthologs
OrthoDB Orthologs (44)
- based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
OrthoDB orthology group searches
Drosophila inclusive ortholog search
Dipteran inclusive ortholog search
Insect inclusive ortholog search
Arthropod inclusive ortholog search
Metazoa inclusive ortholog search
Orthologs in Drosophila Species
(EOG6K9BZF )
Drosophila melanogaster
fruit fly
Drosophila pseudoobscura pseudoobscura
Y
Orthologs in non-Drosophila Dipterans
(EOG6H72MH )
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects
(EOG6K6F16 )
Apis mellifera
Western honey bee
Amel\RpS3A
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG011575
Acromyrmex echinatior
Panamanian leafcutter ant
Aech\AECH21308
Atta cephalotes
Leafcutter ant
Acep\ACEP19752
Camponotus floridanus
Florida carpenter ant
Cflo\CFLO19188
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL16533
Harpegnathos saltator
Jerdons jumping ant
Hsal\HSAL12463
Linepithema humile
Argentine ant
Lhum\LH12841
Pogonomyrmex barbatus
Red harvester ant
Pbar\PB24944
Acyrthosiphon pisum
Pea aphid
Pediculus humanus
Human body louse
Tribolium castaneum
Red flour beetle
Orthologs in non-Insect Arthropods
(EOG66Q58H )
Ixodes scapularis
Deer tick
Orthologs in non-Arthropod Metazoa
(EOG6P8TC8 )
Caenorhabditis elegans
Nematode
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Xenopus tropicalis
Western clawed frog
Gallus gallus
Domestic chicken
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Rattus norvegicus
Norway rat
Human Orthologs (1)AAA Orthologs (10)
based on analysis using Dmel annotation version 4.3
Drosophila pseudoobscura pseudoobscura
Stocks & Reagents Stocks Listed in FlyBase ( 5 )Bloomington VDRC Genomic Clones ( 2 )Please Note FlyBase no
longer curates genomic clone accessions so this list
may not be complete
cDNA Clones ( 991 )Please Note This section lists
cDNAs and ESTs that fall within the genomic extent
of the gene model, which may include cDNAs and ESTs
of genes within introns, or of overlapping genes.
Please see
GBrowse for alignment of the cDNAs and ESTs
to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones Other clones cDNA Clones, End Sequenced
(ESTs) BDGP DGC clones Other clones RNAi & Array Information Linkouts
- Results from RNAi screens.
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Other Information Discoverer Etymology Identification Relationship to Other Genes Source for database identity of Source for identity of: RpS3A CG2168
Source for database merge of Additional comments Formerly designated alleles nearly all deficiencies.
Other Comments RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without
Cdc27 dsRNA.
Deletions removing
RpS3A but no other cytoplasmic ribosomal protein-encoding genes show Minute phenotypes.
Molecularly-defined mutations in
RpS3A result in Minute phenotypes.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
RpS3A has been cloned and sequenced.
One of a class of genes (see MIN record) that when present in one, rather than two, copies, produce a characteristic phenotype consisting of short slender bristles and delayed development.
External Crossreferences & Linkouts
Sequence Crossreferences
- A searchable database of RefSeq genes.
Other Crossreferences
domains - A database of protein families, domains, and functional sites
- Patterns of gene expression in Drosophila embryogenesis
Linkouts
- A database of protein and genetic interactions
- A comprehensive database of gene and protein interactions.
- Results from RNAi screens.
- Cell culture data for RNAi and other high-throughput technologies
- Adult expression by tissue, using Affymetrix Dros2 array
- Integrated genomics database for Drosophila, Anopheles, and C.elegans
- GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents
Protein-protein interactions (PPI) from both known and predicted PPI data sets.
- Data generated by the modENCODE project.
Synonyms & Secondary IDs
( 19 )Reported As Symbol Synonym CG2168
(
Ryder et al., 2004 ,
Cermelli et al., 2006 ,
Goshima et al., 2007 ,
Worringer et al., 2009 ,
Pisa et al., 2009 ,
Keleman et al., 2009.8.5 ,
Marygold et al., 2007 ,
Cammarato et al., 2011 ,
Yano et al., 2012 )
RpS3A
(
Cermelli et al., 2006 ,
Stuart et al., 2007 ,
Goshima et al., 2007 ,
Kallappagoudar et al., 2010 ,
Worringer et al., 2009 ,
Pisa et al., 2009 ,
Nie et al., 2009 ,
Marygold et al., 2007 ,
Schrider et al., 2011 ,
Casad et al., 2011 ,
Japanese National Institute of Genetics, 2012.5.21 ,
Cook et al., 2012 )
Name Synonym Secondary FlyBase
IDs References ( 67 )Generate a list of List References by type
Recent research papers ( 7 )
Cook et al., 2012, Genome Biol. 13(3): R21The generation of chromosomal deletions to provide extensive coverage and subdivision of the Drosophila melanogaster genome. [FBrf0219066] Yano et al., 2012, J. Neurogenet. 26(1): 53--63Identification of proteasome components required for apical localization of chaoptin using functional genomics. [FBrf0217843] Abruzzi et al., 2011, Genes Dev. 25(22): 2374--2386Drosophila CLOCK target gene characterization: implications for circadian tissue-specific gene expression. [FBrf0216798] Booker et al., 2011, BMC Genomics 12: 50False negative rates in Drosophila cell-based RNAi screens: a case study. [FBrf0212969] Casad et al., 2011, Genetics 189(3): 861--870Cardiomyopathy Is Associated with Ribosomal Protein Gene Haplo-Insufficiency in Drosophila melanogaster. [FBrf0216591] Lindquist et al., 2011, Genome Res. 21(3): 433--446Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Schrider et al., 2011, Genome Res. 21(12): 2087--2095Genome-wide analysis of retrogene polymorphisms in Drosophila melanogaster. [FBrf0216827]
Recent reviews (0)
All reviews listed in FlyBase were published before 2011