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General Information
Symbol
Dmel\Rca1
Species
D. melanogaster
Name
Regulator of cyclin A1
Annotation Symbol
CG10800
Feature Type
FlyBase ID
FBgn0017551
Gene Model Status
Stock Availability
Gene Snapshot
Regulator of cyclin A1 (Rca1) encodes an anaphase-promoting complex/cyclosome inhibitor. It contributes to mitotic G1/S transition and cyclin degradation. [Date last reviewed: 2019-07-11]
Also Known As
Emi1
Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:6,852,315..6,853,866 [+]
Recombination map
2-23
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
    -
    Molecular Function (GO)
    [Detailed GO annotations]
    Experimental Evidence
    -
    Predictions / Assertions
    Summaries
    Gene Group (FlyBase)
    F BOX ONLY PROTEINS -
    F box only proteins (FBXO) are F box proteins which do not contain WD40 repeats or leucine-rich repeats, but may contain other domains. F box proteins are the variable substrate adapters for Skp, Cullin, F-box (SCF) E3 ubiquitin ligase complexes. (Adapted from FBrf0225076).
    Summary (Interactive Fly)
    a novel signaling protein that prevents cyclin degredation in G2 by interacting with Fizzy-related - inhibits Fizzy-related's activation of protein degradation machinery - mutants exhibit premature cyclin destruction
    Gene Model and Products
    Number of Transcripts
    1
    Number of Unique Polypeptides
    1

    Please see the GBrowse view of Dmel\Rca1 or the JBrowse view of Dmel\Rca1 for information on other features

    To submit a correction to a gene model please use the Contact FlyBase form

    Protein Domains (via Pfam)
    Isoform displayed:
    Pfam protein domains
    InterPro name
    classification
    start
    end
    Protein Domains (via SMART)
    Isoform displayed:
    SMART protein domains
    InterPro name
    classification
    start
    end
    Comments on Gene Model
    Gene model reviewed during 5.52
    Sequence Ontology: Class of Gene
    Transcript Data
    Annotated Transcripts
    Name
    FlyBase ID
    RefSeq ID
    Length (nt)
    Assoc. CDS (aa)
    FBtr0079342
    1552
    411
    Additional Transcript Data and Comments
    Reported size (kB)
    1.577 (longest cDNA)
    Comments
    External Data
    Crossreferences
    Polypeptide Data
    Annotated Polypeptides
    Name
    FlyBase ID
    Predicted MW (kDa)
    Length (aa)
    Theoretical pI
    RefSeq ID
    GenBank
    FBpp0078970
    46.7
    411
    10.19
    Polypeptides with Identical Sequences

    There is only one protein coding transcript and one polypeptide associated with this gene

    Additional Polypeptide Data and Comments
    Reported size (kDa)
    Comments
    External Data
    Crossreferences
    Linkouts
    Sequences Consistent with the Gene Model
    Mapped Features

    Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rca1 using the Feature Mapper tool.

    External Data
    Crossreferences
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Linkouts
    Gene Ontology (9 terms)
    Molecular Function (1 term)
    Terms Based on Experimental Evidence (0 terms)
    Terms Based on Predictions or Assertions (1 term)
    CV Term
    Evidence
    References
    inferred from sequence model
    Biological Process (6 terms)
    Terms Based on Experimental Evidence (2 terms)
    CV Term
    Evidence
    References
    Terms Based on Predictions or Assertions (4 terms)
    CV Term
    Evidence
    References
    Cellular Component (2 terms)
    Terms Based on Experimental Evidence (1 term)
    CV Term
    Evidence
    References
    inferred from direct assay
    Terms Based on Predictions or Assertions (2 terms)
    CV Term
    Evidence
    References
    inferred from biological aspect of ancestor with PANTHER:PTN000398158
    (assigned by GO_Central )
    Expression Data
    Expression Summary Ribbons
    Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
    For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
    Transcript Expression
    in situ
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    organism

    Comment: maternally deposited

    Additional Descriptive Data
    Rca1 transcripts are abundant and uniformly distributed in early embryos prior to cellularization. During germ band elongation in stages 8 and 9, transcripts are detected predominantly in the mesoderm and in the anterior and posterior midgut primordia. In stages 10 and 11, high levels of Rca1 transcript are observed throughout the embryo but not in the amnioserosa. By stage 13, staining is mainly restricted to the cells of the developing CNS. Weak expression is also observed in the midgut
    Marker for
     
    Subcellular Localization
    CV Term
    Polypeptide Expression
    Additional Descriptive Data
    Marker for
     
    Subcellular Localization
    CV Term
    Evidence
    References
    inferred from direct assay
    Expression Deduced from Reporters
    High-Throughput Expression Data
    Associated Tools

    GBrowse - Visual display of RNA-Seq signals

    View Dmel\Rca1 in GBrowse 2
    RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
    Reference
    See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
    Developmental Proteome: Life Cycle
    Developmental Proteome: Embryogenesis
    External Data and Images
    Linkouts
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Images
    FlyExpress - Embryonic expression images (BDGP data)
    • Stages(s) 1-3
    • Stages(s) 7-8
    • Stages(s) 9-10
    • Stages(s) 13-16
    Alleles, Insertions, and Transgenic Constructs
    Classical and Insertion Alleles ( 12 )
    For All Classical and Insertion Alleles Show
     
    Other relevant insertions
    Transgenic Constructs ( 19 )
    For All Alleles Carried on Transgenic Constructs Show
    Transgenic constructs containing/affecting coding region of Rca1
    Transgenic constructs containing regulatory region of Rca1
    Deletions and Duplications ( 5 )
    Phenotypes
    For more details about a specific phenotype click on the relevant allele symbol.
    Lethality
    Allele
    Sterility
    Allele
    Other Phenotypes
    Allele
    Phenotype manifest in
    Allele
    abdominal segment 2 & cardioblast
    abdominal segment 3 & cardioblast
    abdominal segment 4 & cardioblast
    abdominal segment 5 & cardioblast
    abdominal segment 6 & cardioblast
    abdominal segment 7 & cardioblast
    Orthologs
    Human Orthologs (via DIOPT v7.1)
    Homo sapiens (Human) (2)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    2 of 15
    Yes
    Yes
    2 of 15
    Yes
    Yes
    Model Organism Orthologs (via DIOPT v7.1)
    Mus musculus (laboratory mouse) (2)
    Species\Gene Symbol
    Score
    Best Score
    Best Reverse Score
    Alignment
    Complementation?
    Transgene?
    2 of 15
    Yes
    Yes
    2 of 15
    Yes
    Yes
    Rattus norvegicus (Norway rat) (2)
    2 of 13
    Yes
    Yes
    2 of 13
    Yes
    Yes
    Xenopus tropicalis (Western clawed frog) (2)
    1 of 12
    Yes
    Yes
    1 of 12
    Yes
    Yes
    Danio rerio (Zebrafish) (2)
    2 of 15
    Yes
    Yes
    2 of 15
    Yes
    Yes
    Caenorhabditis elegans (Nematode, roundworm) (0)
    No records found.
    Arabidopsis thaliana (thale-cress) (0)
    No records found.
    Saccharomyces cerevisiae (Brewer's yeast) (0)
    No records found.
    Schizosaccharomyces pombe (Fission yeast) (0)
    No records found.
    Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190C7E )
    Organism
    Common Name
    Gene
    AAA Syntenic Ortholog
    Multiple Dmel Genes in this Orthologous Group
    Drosophila melanogaster
    fruit fly
    Drosophila suzukii
    Spotted wing Drosophila
    Drosophila simulans
    Drosophila sechellia
    Drosophila erecta
    Drosophila yakuba
    Drosophila ananassae
    Drosophila pseudoobscura pseudoobscura
    Drosophila persimilis
    Drosophila persimilis
    Drosophila willistoni
    Drosophila virilis
    Drosophila mojavensis
    Drosophila grimshawi
    Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150J6X )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Culex quinquefasciatus
    Southern house mosquito
    Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0PWW )
    Organism
    Common Name
    Gene
    Multiple Dmel Genes in this Orthologous Group
    Tribolium castaneum
    Red flour beetle
    Tribolium castaneum
    Red flour beetle
    Tribolium castaneum
    Red flour beetle
    Tribolium castaneum
    Red flour beetle
    Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
    No non-Insect Arthropod orthologies identified
    Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
    No non-Arthropod Metazoa orthologies identified
    Paralogs
    Paralogs (via DIOPT v7.1)
    Drosophila melanogaster (Fruit fly) (0)
    No records found.
    Human Disease Associations
    FlyBase Human Disease Model Reports
      Disease Model Summary Ribbon
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Evidence
      References
      Potential Models Based on Orthology ( 0 )
      Human Ortholog
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Allele
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
      Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
      Homo sapiens (Human)
      Gene name
      Score
      OMIM
      OMIM Phenotype
      DO term
      Complementation?
      Transgene?
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Interactions Browser
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement

      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      2L
      Recombination map
      2-23
      Cytogenetic map
      Sequence location
      2L:6,852,315..6,853,866 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      27C1-27C1
      Limits computationally determined from genome sequence between P{lacW}Ate1k10809 and P{PZ}Rca103300&P{lacW}l(2)k09022k09022
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      27B4-27C1
      (determined by in situ hybridisation)
      Experimentally Determined Recombination Data
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (15)
      Genomic Clones (27)
      cDNA Clones (25)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for identity of: Rca1 CG10800
      Source for database merge of
      Additional comments
      Other Comments
      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA.
      numb functions downstream of cell division genes (CycA, Rca1 and stg) and progression through the cell cycle is required for asymmetric localisation of numb and thus N mediated specification of the sib fate in the RP2/sib division.
      The loss of CycA, Rca1 or stg leads to a block in the division of GMC-1, however this GMC-1 adopts a RP2 identity.
      Rca1 wild type function is sufficient to drive postmitotic cells into S phase. Rca1 positively regulates CycA by modulating CycA protein levels.
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 34 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (genetic) - An integrated Molecular Interaction Database
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Synonyms and Secondary IDs (16)
      Reported As
      Symbol Synonym
      l(2)03300
      Name Synonyms
      Regulator of Cyclin A 1
      Suppressor of rux 2A
      regulator of Cyclin A
      regulator of cyclin A
      Secondary FlyBase IDs
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (102)