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Gene Dmel\Rca1

General Information
Symbol	Dmel\Rca1	Species	D. melanogaster
Name	Regulator of cyclin A1	Annotation symbol	CG10800
Feature type	protein_coding_gene	FlyBase ID	FBgn0017551
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	Emi1
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	27C1-27C1	Sequence location	2L:6,852,324..6,853,876 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Regulator of cyclin A1 is referred to in FlyBase by the symbol Dmel\Rca1 (CG10800, FBgn0017551). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. There is experimental evidence that it is involved in the biological process: negative regulation of mitotic anaphase-promoting complex activity; lateral inhibition; G1/S transition of mitotic cell cycle; neurogenesis. 23 alleles are reported. The phenotypes of these alleles are annotated with: anatomical structure; embryonic/larval neuron; multicellular structure; organ system; sense organ; embryonic pericardial cell; external compound sense organ; portion of tissue; cardioblast; adult; organ system subdivision; imaginal precursor. It has one annotated transcript and one annotated polypeptide. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1631034_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Rca1. Gene sequence location is 2L:6852324..6853876.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 27C1-27C1   Limits computationally determined from genome sequence between P{lacW}Ate1k10809 and P{PZ}Rca103300&P{lacW}l(2)k09022k09022
Experimentally Determined Cytological Location
Cytogenetic map Notes References 27B4-27C1     (Dong, 1996.11.6) 27B4-27C1   (determined by in situ hybridisation)   (Spradling et al., 1999) 27B4-27C1     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Dong, 1996.11.6) 2-21 (Dong et al., 1997)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Rca1 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Rca1-RA FBtr0079342  NM_057866   1553   411
Additional Transcript Data & Comments
Reported size (kB)	1.577 (longest cDNA) (Dong et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Rca1-PA   FBpp0078970   46.7   411   10.19     NP_477214   AAF52448
Additional Polypeptide Data & Comments
Reported size (kDa)	412 (aa) (Dong et al., 1997)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA438700   AA538661   AE014134 AAF52448   AC004277   AI238059   AI513287   AQ025609   AY058372 AAL13601   BI213373   BI238099   BQ103587   CO299720   CO300016   CO305584   CO330574   CO333532   CO336293   EC071375   EC199758   EC199784   EC203444   EC204961   EC214396   EC216088   EC216741   EC263245   EV583602   EV585341   EV599044   FE863451   FE863473   FE863545   FE863745   FE863861   FE863932   GH741517   GH762060   GH768935   U77645 AAB39742
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VM76 P91666
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early   organism | ubiquitous   (Dong et al., 1997) embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 8 -- 9   mesoderm   (Dong et al., 1997) embryonic stage 8 -- 17   posterior midgut primordium   (Dong et al., 1997) anterior midgut primordium   (Dong et al., 1997) embryonic stage 9 -- 10   head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   central brain primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) embryonic stage 13 -- 17   presumptive embryonic/larval nervous system   (Dong et al., 1997)
Additional Descriptive Data
	Rca1 transcripts are abundant and uniformly distributed in early embryos prior to cellularization. During germ band elongation in stages 8 and 9, transcripts are detected predominantly in the mesoderm and in the anterior and posterior midgut primordia. In stages 10 and 11, high levels of Rca1 transcript are observed throughout the embryo but not in the amnioserosa. By stage 13, staining is mainly restricted to the cells of the developing CNS. Weak expression is also observed in the midgut (Dong et al., 1997)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary    735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary  (735.1) Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary    735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary    735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary    735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary  735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary    735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    359.35 Larval Midgut    32.2 Larval Hindgut    36.1 Larval Malpighian Tubules    56.1 Larval Fat Body    69.4 Larval Salivary Gland    37 Larval Trachea    58.15 Larval Carcass    43.85 Adult Head    68.1 Adult Eye    41.475 Adult Brain    155.1 Adult Thoracic-Abdominal Ganglion    125.6 Adult Crop    63.4 Adult Midgut    30.6 Adult Hindgut    47.9 Adult Malpighian Tubules    41.3 Adult Fat Body    26.8 Adult Salivary Gland    51.6 Adult Heart    22.925 Adult VirginFemale Spermatheca    29.1 Adult InseminatedFemale Spermatheca    26 Adult Ovary    735.1 Adult Testis    190.8 Adult Male Accessory Gland    41.9 Adult Carcass    48.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    68.1   NA  Eye    41.475   NA  Brain    155.1   359.35  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    125.6   NA  Crop    63.4   no informative data  Midgut    30.6   36.1  Hindgut    47.9   56.1  Malpighian Tubules    41.3   69.4  Fat Body    no informative data   37  Salivary Gland    no informative data   NA  Heart    22.925   58.15  Trachea    NA   NA  VirginFemale Spermatheca    29.1   NA  InseminatedFemale Spermatheca    26   NA  Ovary    735.1   NA  Testis    190.8   NA  Male Accessory Gland    no informative data   43.85  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    77 ovary, mated 4-day female    61 testis, mated 4-day male    34 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3  (32) central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female  (77) ovary, mated 4-day female  (61) testis, mated 4-day male  (34) accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    77 ovary, mated 4-day female    61 testis, mated 4-day male    34 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    77 ovary, mated 4-day female    61 testis, mated 4-day male    34 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    77 ovary, mated 4-day female    61 testis, mated 4-day male    34 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (35) central nervous system, larvae L3  32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female  (77) ovary, mated 4-day female  (61) testis, mated 4-day male  (34) accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    77 ovary, mated 4-day female    61 testis, mated 4-day male    34 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    35 central nervous system, larvae L3    32 central nervous system, pupae P8    9 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    3 head, mated 1-day female    4 head, mated 4-day female    3 head, mated 20-day female    3 head, mated 1-day male    4 head, mated 4-day male    5 head, mated 20-day male    7 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    3 digestive system, larvae L3 wandering    4 digestive system, 1-day adult    2 digestive system, 4-day adult    4 digestive system, 20-day adult    5 fat body, larvae L3 wandering    1 fat body, white prepupae    6 fat body, pupae P8    5 carcass, larvae L3 wandering    13 carcass, 1-day adult    3 carcass, 4-day adult    4 carcass, 20-day adult    5 ovary, virgin 4-day female    77 ovary, mated 4-day female    61 testis, mated 4-day male    34 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    16 embryo 04-06hr    60 embryo 06-08hr    76 embryo 08-10hr    49 embryo 10-12hr    31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day    44 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (83) embryo 02-04hr    16 embryo 04-06hr  (60) embryo 06-08hr  (76) embryo 08-10hr  (49) embryo 10-12hr  (31) embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day  (44) adult female 30day  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    16 embryo 04-06hr    60 embryo 06-08hr    76 embryo 08-10hr    49 embryo 10-12hr    31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day    44 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    16 embryo 04-06hr    60 embryo 06-08hr    76 embryo 08-10hr    49 embryo 10-12hr    31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day    44 adult female 30day    47 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    16 embryo 04-06hr    60 embryo 06-08hr    76 embryo 08-10hr    49 embryo 10-12hr    31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day    44 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (83) embryo 02-04hr    16 embryo 04-06hr  (60) embryo 06-08hr  (76) embryo 08-10hr  (49) embryo 10-12hr  31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day  (44) adult female 30day  (47) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    16 embryo 04-06hr    60 embryo 06-08hr    76 embryo 08-10hr    49 embryo 10-12hr    31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day    44 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    83 embryo 02-04hr    16 embryo 04-06hr    60 embryo 06-08hr    76 embryo 08-10hr    49 embryo 10-12hr    31 embryo 12-14hr    15 embryo 14-16hr    11 embryo 16-18hr    7 embryo 18-20hr    6 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    3 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    11 larva L3 puffstage 7-9    12 white prepupae new    11 white prepupae 12hr    15 white prepupae 24hr    19 pupae 2d postWPP    11 pupae 3d postWPP    6 pupae 4d postWPP    5 adult male 01day    6 adult male 05day    11 adult male 30day    9 adult female 01day    20 adult female 05day    44 adult female 30day    47 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H    26 embryonic GM2    9 embryonic Kc167    8 embryonic S1    30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    36 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H  26 embryonic GM2    9 embryonic Kc167    8 embryonic S1  (30) embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21  (36) wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H    26 embryonic GM2    9 embryonic Kc167    8 embryonic S1    30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    36 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H    26 embryonic GM2    9 embryonic Kc167    8 embryonic S1    30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    36 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H    26 embryonic GM2    9 embryonic Kc167    8 embryonic S1    30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    36 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H  26 embryonic GM2    9 embryonic Kc167    8 embryonic S1  30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21  (36) wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H    26 embryonic GM2    9 embryonic Kc167    8 embryonic S1    30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    36 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    17 Schneider line 2 Sg4    15 embryonic 1182-4H    26 embryonic GM2    9 embryonic Kc167    8 embryonic S1    30 embryonic S3    8 leg disc CME L1    18 wing disc CME-W2    10 wing disc ML-DmD8    16 wing disc ML-DmD9    20 wing disc ML-DmD16-c3    24 wing disc ML-DmD21    36 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    14 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    10 CNS ML-DmBG1-c1    16 CNS ML-DmBG2-c2    8 tumorous blood cells mbn2    16 ovary fGS/OSS    17 ovary OSC    13 ovary OSS    6 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (46) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (53) Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult  (35) Paraquat 10 mM 48 hrs, 4-day adult  (29) Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult  (44) Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3  (46) Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (53) Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult  (35) Paraquat 10 mM 48 hrs, 4-day adult  29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    18 cold shock, 4-day adult    9 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    3 Cadmium 50 mM 12 hrs, larvae L3    7 Cadmium 50 mM 48 hrs, 4-day adult    17 Cadmium 100 mM 48 hrs, 4-day adult    44 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    23 Zinc 5 mM 12 hrs, larvae L3    5 Zinc 4.5 mM 48 hrs, 4-day adult    6 Ethanol 2.5% 3 hrs, larvae L3    46 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    6 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    53 Caffeine 25 mg/ml 48 hrs, 4-day adult    15 Paraquat 5 mM 48 hrs, 4-day adult    35 Paraquat 10 mM 48 hrs, 4-day adult    29 Rotenone 2 μg 12 hrs, larvae L3    5 Rotenone 8 μg 12 hrs, larvae L3    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_06 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • GH14043 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0017551 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10800-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Rca1 Stages(s) 1-3 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Rca1GD12609 lethal | pupal stage, with Scer\GAL4arm.PS Rca1Scer\UAS.T:Ivir\HA1 lethal | recessive Rca103300 partially lethal - majority die Rca103300 viable Rca1e02614 viable, with Scer\GAL4pnr-MD237 Rca1GD12609 viable, with Scer\GAL4prd.RG1 Rca1Scer\UAS.T:Ivir\HA1 Sterility Allele fertile Rca1e02614 Other Phenotypes Allele decreased cell number | embryonic cycle 16 Rca12 increased cell number, with Scer\GAL4GMR.PU Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG mitotic cell cycle defective Rca12 Rca13 Rca1GMR.PD Rca1hs.PD mitotic cell cycle defective (with Rca12) Rca13 mitotic cell cycle defective (with Rca13) Rca12 mitotic cell cycle defective | embryonic cycle 16 Rca12 mitotic cell cycle defective | somatic clone Rca12 Rca13 mitotic cell cycle defective | somatic clone, with Scer\GAL4Act5C.PI Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG neuroanatomy defective | larval stage Rca1GD12609 visible, with Scer\GAL4pnr-MD237 Rca1GD12609 Phenotype manifest in Allele abdominal segment 2 & cardioblast Rca103300 abdominal segment 3 & cardioblast Rca103300 abdominal segment 4 & cardioblast Rca103300 abdominal segment 5 & cardioblast Rca103300 abdominal segment 6 & cardioblast Rca103300 abdominal segment 7 & cardioblast Rca103300 aCC neuron | precursor Rca103300 cardioblast Rca1unspecified chaeta, with Scer\GAL4pnr-MD237 Rca1GD12609 EL neuron Rca103300 Rca133X16 embryonic/larval neuroblast | larval stage Rca1GD12609 embryonic pericardial cell Rca103300 eye Rca1GMR.PD Rca1hs.PD eye, with Scer\GAL4GMR.PU Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG eye disc posterior to the morphogenetic furrow, with Scer\GAL4GMR.PU Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG eye disc posterior to the morphogenetic furrow | somatic clone, with Scer\GAL4GMR.PU Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG ganglion mother cell Rca103300 ganglion mother cell | larval stage Rca1GD12609 ganglion mother cell GMC1-1a Rca103300 Rca133X16 ganglion mother cell GMC4-2a Rca103300 Rca133X16 nucleus | somatic clone Rca12 pCC neuron Rca103300 pCC neuron | precursor Rca103300 RP2sib neuron Rca103300 U neuron Rca103300 wing disc | somatic clone Rca12
Classical Alleles ( 10 )
For All Classical Alleles Show
Allele of Rca1 Class Mutagen Stocks Known lesion Rca103300 P-element activity 2 Yes Rca1e02614 piggyBac transposase 2 -- Rca12 1 -- Rca1IX loss of function allele 1 -- Rca111294 0 -- Rca11 ethyl methanesulfonate 0 Yes Rca133X16 hypomorphic allele - genetic evidence P-element activity 0 -- Rca13 0 -- Rca1S(rux)2A ethyl methanesulfonate X ray 0 -- Rca1unspecified 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Rca1 Class Mutagen Stocks Known lesion Rca1GD12609 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Rca1hs.PD in vitro construct - regulatory fusion 2 Yes Rca1+t10 in vitro construct - genomic fragment 0 Yes Rca1C351S.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - amino acid replacement 0 Yes Rca1GMR.PD in vitro construct - regulatory fusion 0 Yes Rca1NIG.10800R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Rca1Scer\UAS.P\T.cNa 0 Yes Rca1Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rca1Scer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rca1Δ133.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rca1Δ203.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rca1Δ255.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Rca1ΔF-box.Scer\UAS.T:Ivir\HA1.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)Dwee1 (BDGP Project Members, 1994-1999) Df(2L)Dwee-δ5 (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Not disrupted in	Df(2L)Dwee1-W05 (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)J-H (Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)spd (BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12609} NA P{NIG.10800R} NA P{UAS-Rca1.HA} NA heat-shock construct P{hs-rca1} NA characterization construct P{pGMR-rca1} NA P{rca1} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{PZ}Rca103300 No miscellaneous insertions PBac{RB}Rca1e02614 NA
Gene Ontology: Function, Process & Cellular Component ( 6 unique terms )
Terms Based on Experimental Evidence ( 4 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
G1/S transition of mitotic cell cycle inferred from mutant phenotype (Zielke et al., 2006) lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) negative regulation of mitotic anaphase-promoting complex activity inferred from mutant phenotype (Zielke et al., 2006) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
eye-antennal disc morphogenesis non-traceable author statement (Baker, 2001) regulation of mitosis traceable author statement (Lee and Orr-Weaver, 2003)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Rca1 - CG10077 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - CG11876 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - CG3817 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - CG4553 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - CG9769 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Clbn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Imp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Int6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Mtpα anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - NHP2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Ote anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - RpS11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - RpS19b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Rs1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Rsf1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - ctp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - eIF3-S8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpL15 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpL22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpL43 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpL50 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpL52 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - mTerf3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Rca1 - skpA anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Rca1 allele Gene References Rca133X16 numb (Lear et al., 1999) Rca103300 CycE (Secombe et al., 1998) Rca1S(rux)2A rux (Dong et al., 1997) Rca1Scer\UAS.T:Ivir\HA1 rap (Grosskortenhaus and Sprenger, 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60110 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0017551 DroID - A comprehensive database of gene and protein interactions. • FBgn0017551 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33959
Orthologs
OrthoDB Orthologs (14) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6RXZK4 Dipteran inclusive ortholog search EOG6HX58N Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (EOG6RXZK4)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Rca1     Drosophila simulans   Dsim\GD23434 Y   Drosophila sechellia   Dsec\GM16226 Y   Drosophila erecta   Dere\GG10438 Y   Drosophila yakuba   Dyak\GE14154 Y   Drosophila ananassae   Dana\GF15431 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10566 Y   Drosophila persimilis   Dper\GL26189 Y   Drosophila persimilis   Dper\GL23201     Drosophila willistoni   Dwil\GK24784 Y   Drosophila virilis   Dvir\GJ21279 Y   Drosophila mojavensis   Dmoj\GI23995 Y   Drosophila grimshawi   Dgri\GH13716 Y
Orthologs in non-Drosophila Dipterans (EOG6HX58N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ017739
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23434 Drosophila sechellia Dsec\GM16226 Drosophila erecta Dere\GG10438 Drosophila yakuba Dyak\GE14154 Drosophila ananassae Dana\GF15431 Drosophila pseudoobscura pseudoobscura Dpse\GA10566 Drosophila persimilis Dper\GL26189 Drosophila willistoni Dwil\GK24784 Drosophila virilis Dvir\GJ21279 Drosophila mojavensis Dmoj\GI23995 Drosophila grimshawi Dgri\GH13716
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	9163 w*; P{hs-rca1}I4 9164 w*; P{hs-rca1}C2 18068 w1118; PBac{RB}Rca1e02614 9161 Rca12 cn1 bw1/SM6a 9162 Rca1IX cn1 bw1/SM6a 11294 P{PZ}Rca103300 cn1/CyO; ry506
Harvard	- PBac{RB}Rca1e02614
Kyoto	108868 P{PZ}Rca103300 cn1/CyO; ry506
VDRC	v35489 w1118; P{GD12609}v35489 v35490 w1118; P{GD12609}v35490
Genomic Clones ( 1 )
	BACR20B21 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 25 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH14043
Other clones	DPiM_GH14043
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD13031 LD18242 RE18759 RE34476
Other clones	Dmel_dig_001817_0623_2926 Dmel_dig_003299_0657_1339 Dmel_neb_008642_0935_1819 EK176412 EK177231 EK249017 EK295618 EK307213 EN12236 RM07478 RM07678 RM07778 RM07978 RM08078 RM08178 RP001003768 RP001026746 RP001026846 UUGC0995
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0017551 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33959
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Rca1 CG10800 (Li, 1999.11)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly long spindles when assayed in S2 cells in the presence of Cdc27 dsRNA. This phenotype cannot be observed when the screen is performed without Cdc27 dsRNA. (Goshima et al., 2007) numb functions downstream of cell division genes (CycA, Rca1 and stg) and progression through the cell cycle is required for asymmetric localisation of numb and thus N mediated specification of the sib fate in the RP2/sib division. (Wai et al., 1999) The loss of CycA, Rca1 or stg leads to a block in the division of GMC-1, however this GMC-1 adopts a RP2 identity. (Wai et al., 1999) Rca1 wild type function is sufficient to drive postmitotic cells into S phase. Rca1 positively regulates CycA by modulating CycA protein levels. (Dong et al., 1997)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057866 RefSeq (Proteins) • NP_477214 DDBJ / EMBL / GenBank • AA438700 AAB39742 AAF52448 AAL13601 AC004277 AI238059 AI513287 AQ025609 AY058372 U77645 Entrez Gene - A searchable database of RefSeq genes. • 33959 UniProtKB/TrEMBL • P91666 Q9VM76
Other Crossreferences
	BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH14043
Linkouts
	BioGRID - A database of protein and genetic interactions • 60110 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0017551 DroID - A comprehensive database of gene and protein interactions. • FBgn0017551 DRSC - Results from RNAi screens. • FBgn0017551 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0017551 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10800-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0017551 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33959 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/regcyca1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33959 modMine - Data generated by the modENCODE project. • FBgn0017551
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	152549_at (Kristensen et al., 2005) CG10800 (Labourier et al., 2002, Goshima et al., 2007, Roberts et al., 2012) CLD4 (Erhardt et al., 2008) Emi1 (Tanaka-Matakatsu et al., 2007, Buttitta et al., 2010) FBXO5 (Roberts et al., 2012) l(2)03300   P3300 (Lear and Patel, 1997) rca1 (Fichelson et al., 2005, Zielke and Sprenger, 2003, Zielke and Sprenger, 2003, Lee and Orr-Weaver, 2003, Grosskortenhaus and Sprenger, 2002, Baker, 2001, Lee et al., 2001, de Nooij et al., 2000, Dong, 1996.11.6, Lear et al., 1999, Wai et al., 1999, Truong et al., 1999, Katzen et al., 1999, Secombe et al., 1998, Lear and Patel, 1998, Dong et al., 1997, Sandmann et al., 2007, Narbonne-Reveau et al., 2008, Zielke et al., 2008, Rogers et al., 2009, Zielke et al., 2006, Heriche et al., 2003, Tanaka-Matakatsu et al., 2007, Roberts et al., 2012) Rca1 (Pines, 2006, Friedman and Perrimon, 2006, Goshima et al., 2007, Lindquist et al., 2011, Chen et al., 2008, Liu et al., 2009, Zielke et al., 2006, Narbonne-Reveau et al., 2008, Buttitta et al., 2010, Neumüller et al., 2011, Kaplow et al., 2007, Japanese National Institute of Genetics, 2012.5.21) RCA1 (Acquaviva and Pines, 2006, Erhardt et al., 2008) S(rux)2A (Dong et al., 1997)
Name Synonym	regulator of cyclin A (Dong et al., 1997) regulator of Cyclin A (Zielke et al., 2006) Regulator of cyclin A1 (Kaplow et al., 2007) Regulator of Cyclin A 1 (Narbonne-Reveau et al., 2008) Suppressor of rux 2A (Dong et al., 1997)
Secondary FlyBase IDs
References ( 75 )
Generate a list of	All references relating to this gene ( 75 )
List References by type	Research paper  ( 38 ) Supplementary material  ( 6 ) Review  ( 8 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 10 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 1 ) Stock list  ( 1 ) Curated genome annotation  ( 1 ) Website  ( 1 )
Recent research papers ( 5 )
	Dui et al., 2012, J. Genet. Genomics 39(8): 397--413 A Systematic Phenotypic Screen of F-box Genes Through a Tissue-specific RNAi-based Approach in Drosophila. [FBrf0219135] Roberts et al., 2012, PLoS ONE 7(2): e31284 Defining Components of the ßcatenin Destruction Complex and Exploring Its Regulation and Mechanisms of Action during Development. [FBrf0217546] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Zielke et al., 2011, Nature 480(7375): 123--127 Control of Drosophila endocycles by E2F and CRL4CDT2. [FBrf0216829] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...