Gene Dmel\RpL14

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General Information

Symbol

Dmel\RpL14

Species

D. melanogaster

Name

Ribosomal protein L14

Annotation symbol

CG6253

Feature type

protein_coding_gene

FlyBase ID

FBgn0017579

Gene Model Status

Current

Stock availability

5 publicly available

Also Known As

M(3)66D, L14

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

66D8-66D8

Sequence location

3L:8,594,106..8,595,492 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene Ribosomal protein L14 is referred to in FlyBase by the symbol Dmel\RpL14 (CG6253, FBgn0017579). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: structural constituent of ribosome. There is experimental evidence that it is involved in the biological process: centrosome organization; mitotic spindle elongation; negative regulation of neuron apoptotic process; mitotic spindle organization; centrosome duplication. 9 alleles are reported. The phenotypes of these alleles are annotated with: organ system subdivision; adult segment; external compound sense organ; organ system; adult; thoracic segment; sense organ; imaginal precursor; peripheral nervous system; embryonic/larval imaginal precursor. It has one annotated transcript and one annotated polypeptide. Protein features are: Ribosomal protein L14; Ribosomal protein L2 domain 2. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, during early pupal stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1623914_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of RpL14. Gene sequence location is 3L:8594106..8595492.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

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FB2013_03

FB2013_02

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Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

66D8-66D8

Limits computationally determined from genome sequence between P{lacW}foi^j8E8 and P{PZ}SrpRβ^rK561&P{lacW}l(3)j5B6^j5B6

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

66D1-66D15

(Saeboe-Larssen, 1996.12.11)

66D-66D

(determined by in situ hybridisation)

(Saeboe-Larssen et al., 1997)

Experimentally Determined Recombination Data

Location

(Saeboe-Larssen, 1996.12.11)

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\RpL14 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.44

Gene model reviewed during 5.46

Low-frequency RNA-Seq exon junction(s) not annotated.

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

RpL14-RA

FBtr0076633

NM_079251

619

166

Additional Transcript Data & Comments

Reported size (kB)

0.65 (unknown)

(Saeboe-Larssen et al., 1997)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

RpL14-PA

FBpp0076359

19.2

166

11.79

NP_523975

AAF50393

Additional Polypeptide Data & Comments

Reported size (kDa)

166 (aa); 19 (kD)

(Saeboe-Larssen et al., 1997)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Ribosomal protein L14 (IPR002784)

Ribosomal protein L2 domain 2 (IPR014722)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

P55841

UniProtKB/TrEMBL

Q5BIL1

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Saeboe-Larssen et al., 1997)

Additional Descriptive Data

RpL14 transcripts are detected throughout development on northern blots.

(Saeboe-Larssen et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

ribosome

inferred from direct assay

(Alonso and Santaren, 2006)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2675.525
Larval Midgut		4537.2
Larval Hindgut		4333.8
Larval Malpighian Tubules		4626
Larval Fat Body		7323.7
Larval Salivary Gland		7621.1
Larval Trachea		5695.275
Larval Carcass		4558.575
Adult Head		4300.4
Adult Eye		5588.125
Adult Brain		2967.5
Adult Thoracic-Abdominal Ganglion		4174.3
Adult Crop		5760.9
Adult Midgut		4941.4
Adult Hindgut		4660.5
Adult Malpighian Tubules		2583.9
Adult Fat Body		5606.3
Adult Salivary Gland		10085.7
Adult Heart		6348.5
Adult VirginFemale Spermatheca		4075.5
Adult InseminatedFemale Spermatheca		4374.9
Adult Ovary		3256.1
Adult Testis		1003.8
Adult Male Accessory Gland		8232.7
Adult Carcass		5461.4
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(2675.525)
Larval Midgut		(4537.2)
Larval Hindgut		(4333.8)
Larval Malpighian Tubules		(4626)
Larval Fat Body		(7323.7)
Larval Salivary Gland		(7621.1)
Larval Trachea		(5695.275)
Larval Carcass		(4558.575)
Adult Head		(4300.4)
Adult Eye		(5588.125)
Adult Brain		(2967.5)
Adult Thoracic-Abdominal Ganglion		(4174.3)
Adult Crop		(5760.9)
Adult Midgut		(4941.4)
Adult Hindgut		(4660.5)
Adult Malpighian Tubules		(2583.9)
Adult Fat Body		(5606.3)
Adult Salivary Gland		(10085.7)
Adult Heart		(6348.5)
Adult VirginFemale Spermatheca		(4075.5)
Adult InseminatedFemale Spermatheca		(4374.9)
Adult Ovary		(3256.1)
Adult Testis		(1003.8)
Adult Male Accessory Gland		(8232.7)
Adult Carcass		(5461.4)
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(2675.525)
Larval Midgut		(4537.2)
Larval Hindgut		(4333.8)
Larval Malpighian Tubules		(4626)
Larval Fat Body		(7323.7)
Larval Salivary Gland		(7621.1)
Larval Trachea		(5695.275)
Larval Carcass		(4558.575)
Adult Head		(4300.4)
Adult Eye		(5588.125)
Adult Brain		(2967.5)
Adult Thoracic-Abdominal Ganglion		(4174.3)
Adult Crop		(5760.9)
Adult Midgut		(4941.4)
Adult Hindgut		(4660.5)
Adult Malpighian Tubules		(2583.9)
Adult Fat Body		(5606.3)
Adult Salivary Gland		(10085.7)
Adult Heart		(6348.5)
Adult VirginFemale Spermatheca		(4075.5)
Adult InseminatedFemale Spermatheca		(4374.9)
Adult Ovary		(3256.1)
Adult Testis		1003.8
Adult Male Accessory Gland		(8232.7)
Adult Carcass		(5461.4)
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2675.525
Larval Midgut		4537.2
Larval Hindgut		4333.8
Larval Malpighian Tubules		4626
Larval Fat Body		7323.7
Larval Salivary Gland		7621.1
Larval Trachea		5695.275
Larval Carcass		4558.575
Adult Head		4300.4
Adult Eye		5588.125
Adult Brain		2967.5
Adult Thoracic-Abdominal Ganglion		4174.3
Adult Crop		5760.9
Adult Midgut		4941.4
Adult Hindgut		4660.5
Adult Malpighian Tubules		2583.9
Adult Fat Body		5606.3
Adult Salivary Gland		10085.7
Adult Heart		6348.5
Adult VirginFemale Spermatheca		4075.5
Adult InseminatedFemale Spermatheca		4374.9
Adult Ovary		3256.1
Adult Testis		1003.8
Adult Male Accessory Gland		8232.7
Adult Carcass		5461.4
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		2675.525
Larval Midgut		4537.2
Larval Hindgut		4333.8
Larval Malpighian Tubules		4626
Larval Fat Body		7323.7
Larval Salivary Gland		7621.1
Larval Trachea		5695.275
Larval Carcass		4558.575
Adult Head		4300.4
Adult Eye		5588.125
Adult Brain		2967.5
Adult Thoracic-Abdominal Ganglion		4174.3
Adult Crop		5760.9
Adult Midgut		4941.4
Adult Hindgut		4660.5
Adult Malpighian Tubules		2583.9
Adult Fat Body		5606.3
Adult Salivary Gland		10085.7
Adult Heart		6348.5
Adult VirginFemale Spermatheca		4075.5
Adult InseminatedFemale Spermatheca		4374.9
Adult Ovary		3256.1
Adult Testis		1003.8
Adult Male Accessory Gland		8232.7
Adult Carcass		5461.4
Expression Level Scale		None Low Moderate High Very high

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		(2675.525)
Larval Midgut		(4537.2)
Larval Hindgut		(4333.8)
Larval Malpighian Tubules		(4626)
Larval Fat Body		(7323.7)
Larval Salivary Gland		(7621.1)
Larval Trachea		(5695.275)
Larval Carcass		(4558.575)
Adult Head		(4300.4)
Adult Eye		(5588.125)
Adult Brain		(2967.5)
Adult Thoracic-Abdominal Ganglion		(4174.3)
Adult Crop		(5760.9)
Adult Midgut		(4941.4)
Adult Hindgut		(4660.5)
Adult Malpighian Tubules		(2583.9)
Adult Fat Body		(5606.3)
Adult Salivary Gland		(10085.7)
Adult Heart		(6348.5)
Adult VirginFemale Spermatheca		(4075.5)
Adult InseminatedFemale Spermatheca		(4374.9)
Adult Ovary		(3256.1)
Adult Testis		(1003.8)
Adult Male Accessory Gland		(8232.7)
Adult Carcass		(5461.4)
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		(2675.525)
Larval Midgut		(4537.2)
Larval Hindgut		(4333.8)
Larval Malpighian Tubules		(4626)
Larval Fat Body		(7323.7)
Larval Salivary Gland		(7621.1)
Larval Trachea		(5695.275)
Larval Carcass		(4558.575)
Adult Head		(4300.4)
Adult Eye		(5588.125)
Adult Brain		(2967.5)
Adult Thoracic-Abdominal Ganglion		(4174.3)
Adult Crop		(5760.9)
Adult Midgut		(4941.4)
Adult Hindgut		(4660.5)
Adult Malpighian Tubules		(2583.9)
Adult Fat Body		(5606.3)
Adult Salivary Gland		(10085.7)
Adult Heart		(6348.5)
Adult VirginFemale Spermatheca		(4075.5)
Adult InseminatedFemale Spermatheca		(4374.9)
Adult Ovary		(3256.1)
Adult Testis		1003.8
Adult Male Accessory Gland		(8232.7)
Adult Carcass		(5461.4)
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		2675.525
Larval Midgut		4537.2
Larval Hindgut		4333.8
Larval Malpighian Tubules		4626
Larval Fat Body		7323.7
Larval Salivary Gland		7621.1
Larval Trachea		5695.275
Larval Carcass		4558.575
Adult Head		4300.4
Adult Eye		5588.125
Adult Brain		2967.5
Adult Thoracic-Abdominal Ganglion		4174.3
Adult Crop		5760.9
Adult Midgut		4941.4
Adult Hindgut		4660.5
Adult Malpighian Tubules		2583.9
Adult Fat Body		5606.3
Adult Salivary Gland		10085.7
Adult Heart		6348.5
Adult VirginFemale Spermatheca		4075.5
Adult InseminatedFemale Spermatheca		4374.9
Adult Ovary		3256.1
Adult Testis		1003.8
Adult Male Accessory Gland		8232.7
Adult Carcass		5461.4
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	4300.4
NA	Eye	5588.125
NA	Brain	2967.5
2675.525	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	4174.3
NA	Crop	5760.9
4537.2	Midgut	4941.4
4333.8	Hindgut	4660.5
4626	Malpighian Tubules	2583.9
7323.7	Fat Body	5606.3
7621.1	Salivary Gland	10085.7
NA	Heart	6348.5
5695.275	Trachea	NA
NA	VirginFemale Spermatheca	4075.5
NA	InseminatedFemale Spermatheca	4374.9
NA	Ovary	3256.1
NA	Testis	1003.8
NA	Male Accessory Gland	8232.7
4558.575	Carcass	5461.4

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1650
central nervous system, larvae L3		989
central nervous system, pupae P8		406
head, virgin 1-day female		697
head, virgin 4-day female		1084
head, virgin 20-day female		1379
head, mated 1-day female		587
head, mated 4-day female		721
head, mated 20-day female		1056
head, mated 1-day male		682
head, mated 4-day male		514
head, mated 20-day male		761
salivary gland, larvae L3 wandering		527
salivary gland, white prepupae		1435
digestive system, larvae L3 wandering		875
digestive system, 1-day adult		797
digestive system, 4-day adult		600
digestive system, 20-day adult		784
fat body, larvae L3 wandering		609
fat body, white prepupae		463
fat body, pupae P8		1211
carcass, larvae L3 wandering		1088
carcass, 1-day adult		565
carcass, 4-day adult		641
carcass, 20-day adult		992
ovary, virgin 4-day female		1200
ovary, mated 4-day female		1108
testis, mated 4-day male		522
accessory gland, mated 4-day male		877
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(1650)
central nervous system, larvae L3		(989)
central nervous system, pupae P8		(406)
head, virgin 1-day female		(697)
head, virgin 4-day female		(1084)
head, virgin 20-day female		(1379)
head, mated 1-day female		(587)
head, mated 4-day female		(721)
head, mated 20-day female		(1056)
head, mated 1-day male		(682)
head, mated 4-day male		(514)
head, mated 20-day male		(761)
salivary gland, larvae L3 wandering		(527)
salivary gland, white prepupae		(1435)
digestive system, larvae L3 wandering		(875)
digestive system, 1-day adult		(797)
digestive system, 4-day adult		(600)
digestive system, 20-day adult		(784)
fat body, larvae L3 wandering		(609)
fat body, white prepupae		(463)
fat body, pupae P8		(1211)
carcass, larvae L3 wandering		(1088)
carcass, 1-day adult		(565)
carcass, 4-day adult		(641)
carcass, 20-day adult		(992)
ovary, virgin 4-day female		(1200)
ovary, mated 4-day female		(1108)
testis, mated 4-day male		(522)
accessory gland, mated 4-day male		(877)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(1650)
central nervous system, larvae L3		(989)
central nervous system, pupae P8		(406)
head, virgin 1-day female		(697)
head, virgin 4-day female		(1084)
head, virgin 20-day female		(1379)
head, mated 1-day female		(587)
head, mated 4-day female		(721)
head, mated 20-day female		(1056)
head, mated 1-day male		(682)
head, mated 4-day male		(514)
head, mated 20-day male		(761)
salivary gland, larvae L3 wandering		(527)
salivary gland, white prepupae		(1435)
digestive system, larvae L3 wandering		(875)
digestive system, 1-day adult		(797)
digestive system, 4-day adult		(600)
digestive system, 20-day adult		(784)
fat body, larvae L3 wandering		(609)
fat body, white prepupae		(463)
fat body, pupae P8		(1211)
carcass, larvae L3 wandering		(1088)
carcass, 1-day adult		(565)
carcass, 4-day adult		(641)
carcass, 20-day adult		(992)
ovary, virgin 4-day female		(1200)
ovary, mated 4-day female		(1108)
testis, mated 4-day male		(522)
accessory gland, mated 4-day male		(877)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1650
central nervous system, larvae L3		989
central nervous system, pupae P8		406
head, virgin 1-day female		697
head, virgin 4-day female		1084
head, virgin 20-day female		1379
head, mated 1-day female		587
head, mated 4-day female		721
head, mated 20-day female		1056
head, mated 1-day male		682
head, mated 4-day male		514
head, mated 20-day male		761
salivary gland, larvae L3 wandering		527
salivary gland, white prepupae		1435
digestive system, larvae L3 wandering		875
digestive system, 1-day adult		797
digestive system, 4-day adult		600
digestive system, 20-day adult		784
fat body, larvae L3 wandering		609
fat body, white prepupae		463
fat body, pupae P8		1211
carcass, larvae L3 wandering		1088
carcass, 1-day adult		565
carcass, 4-day adult		641
carcass, 20-day adult		992
ovary, virgin 4-day female		1200
ovary, mated 4-day female		1108
testis, mated 4-day male		522
accessory gland, mated 4-day male		877
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1650
central nervous system, larvae L3		989
central nervous system, pupae P8		406
head, virgin 1-day female		697
head, virgin 4-day female		1084
head, virgin 20-day female		1379
head, mated 1-day female		587
head, mated 4-day female		721
head, mated 20-day female		1056
head, mated 1-day male		682
head, mated 4-day male		514
head, mated 20-day male		761
salivary gland, larvae L3 wandering		527
salivary gland, white prepupae		1435
digestive system, larvae L3 wandering		875
digestive system, 1-day adult		797
digestive system, 4-day adult		600
digestive system, 20-day adult		784
fat body, larvae L3 wandering		609
fat body, white prepupae		463
fat body, pupae P8		1211
carcass, larvae L3 wandering		1088
carcass, 1-day adult		565
carcass, 4-day adult		641
carcass, 20-day adult		992
ovary, virgin 4-day female		1200
ovary, mated 4-day female		1108
testis, mated 4-day male		522
accessory gland, mated 4-day male		877
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(1650)
central nervous system, larvae L3		(989)
central nervous system, pupae P8		(406)
head, virgin 1-day female		(697)
head, virgin 4-day female		(1084)
head, virgin 20-day female		(1379)
head, mated 1-day female		(587)
head, mated 4-day female		(721)
head, mated 20-day female		(1056)
head, mated 1-day male		(682)
head, mated 4-day male		(514)
head, mated 20-day male		(761)
salivary gland, larvae L3 wandering		(527)
salivary gland, white prepupae		(1435)
digestive system, larvae L3 wandering		(875)
digestive system, 1-day adult		(797)
digestive system, 4-day adult		(600)
digestive system, 20-day adult		(784)
fat body, larvae L3 wandering		(609)
fat body, white prepupae		(463)
fat body, pupae P8		(1211)
carcass, larvae L3 wandering		(1088)
carcass, 1-day adult		(565)
carcass, 4-day adult		(641)
carcass, 20-day adult		(992)
ovary, virgin 4-day female		(1200)
ovary, mated 4-day female		(1108)
testis, mated 4-day male		(522)
accessory gland, mated 4-day male		(877)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(1650)
central nervous system, larvae L3		(989)
central nervous system, pupae P8		(406)
head, virgin 1-day female		(697)
head, virgin 4-day female		(1084)
head, virgin 20-day female		(1379)
head, mated 1-day female		(587)
head, mated 4-day female		(721)
head, mated 20-day female		(1056)
head, mated 1-day male		(682)
head, mated 4-day male		(514)
head, mated 20-day male		(761)
salivary gland, larvae L3 wandering		(527)
salivary gland, white prepupae		(1435)
digestive system, larvae L3 wandering		(875)
digestive system, 1-day adult		(797)
digestive system, 4-day adult		(600)
digestive system, 20-day adult		(784)
fat body, larvae L3 wandering		(609)
fat body, white prepupae		(463)
fat body, pupae P8		(1211)
carcass, larvae L3 wandering		(1088)
carcass, 1-day adult		(565)
carcass, 4-day adult		(641)
carcass, 20-day adult		(992)
ovary, virgin 4-day female		(1200)
ovary, mated 4-day female		(1108)
testis, mated 4-day male		(522)
accessory gland, mated 4-day male		(877)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		1650
central nervous system, larvae L3		989
central nervous system, pupae P8		406
head, virgin 1-day female		697
head, virgin 4-day female		1084
head, virgin 20-day female		1379
head, mated 1-day female		587
head, mated 4-day female		721
head, mated 20-day female		1056
head, mated 1-day male		682
head, mated 4-day male		514
head, mated 20-day male		761
salivary gland, larvae L3 wandering		527
salivary gland, white prepupae		1435
digestive system, larvae L3 wandering		875
digestive system, 1-day adult		797
digestive system, 4-day adult		600
digestive system, 20-day adult		784
fat body, larvae L3 wandering		609
fat body, white prepupae		463
fat body, pupae P8		1211
carcass, larvae L3 wandering		1088
carcass, 1-day adult		565
carcass, 4-day adult		641
carcass, 20-day adult		992
ovary, virgin 4-day female		1200
ovary, mated 4-day female		1108
testis, mated 4-day male		522
accessory gland, mated 4-day male		877
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of extremely high expression to a trough of very high expression. Peak expression observed at stages throughout embryogenesis, at stages throughout the larval period, during early pupal stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		2062
embryo 02-04hr		2812
embryo 04-06hr		3114
embryo 06-08hr		3199
embryo 08-10hr		3880
embryo 10-12hr		4672
embryo 12-14hr		2558
embryo 14-16hr		2881
embryo 16-18hr		2428
embryo 18-20hr		2985
embryo 20-22hr		2824
embryo 22-24hr		2498
larva L1		3619
larva L2		2990
larva L3 12hr old		2362
larva L3 puffstage 1-2		1991
larva L3 puffstage 3-6		1819
larva L3 puffstage 7-9		1954
white prepupae new		1608
white prepupae 12hr		1501
white prepupae 24hr		1397
pupae 2d postWPP		1230
pupae 3d postWPP		1047
pupae 4d postWPP		1265
adult male 01day		935
adult male 05day		979
adult male 30day		967
adult female 01day		2256
adult female 05day		2075
adult female 30day		2277
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(2062)
embryo 02-04hr		(2812)
embryo 04-06hr		(3114)
embryo 06-08hr		(3199)
embryo 08-10hr		(3880)
embryo 10-12hr		(4672)
embryo 12-14hr		(2558)
embryo 14-16hr		(2881)
embryo 16-18hr		(2428)
embryo 18-20hr		(2985)
embryo 20-22hr		(2824)
embryo 22-24hr		(2498)
larva L1		(3619)
larva L2		(2990)
larva L3 12hr old		(2362)
larva L3 puffstage 1-2		(1991)
larva L3 puffstage 3-6		(1819)
larva L3 puffstage 7-9		(1954)
white prepupae new		(1608)
white prepupae 12hr		(1501)
white prepupae 24hr		(1397)
pupae 2d postWPP		(1230)
pupae 3d postWPP		(1047)
pupae 4d postWPP		(1265)
adult male 01day		(935)
adult male 05day		(979)
adult male 30day		(967)
adult female 01day		(2256)
adult female 05day		(2075)
adult female 30day		(2277)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(2062)
embryo 02-04hr		(2812)
embryo 04-06hr		(3114)
embryo 06-08hr		(3199)
embryo 08-10hr		(3880)
embryo 10-12hr		(4672)
embryo 12-14hr		(2558)
embryo 14-16hr		(2881)
embryo 16-18hr		(2428)
embryo 18-20hr		(2985)
embryo 20-22hr		(2824)
embryo 22-24hr		(2498)
larva L1		(3619)
larva L2		(2990)
larva L3 12hr old		(2362)
larva L3 puffstage 1-2		(1991)
larva L3 puffstage 3-6		(1819)
larva L3 puffstage 7-9		(1954)
white prepupae new		(1608)
white prepupae 12hr		(1501)
white prepupae 24hr		(1397)
pupae 2d postWPP		(1230)
pupae 3d postWPP		(1047)
pupae 4d postWPP		(1265)
adult male 01day		(935)
adult male 05day		(979)
adult male 30day		(967)
adult female 01day		(2256)
adult female 05day		(2075)
adult female 30day		(2277)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		2062
embryo 02-04hr		2812
embryo 04-06hr		3114
embryo 06-08hr		3199
embryo 08-10hr		3880
embryo 10-12hr		4672
embryo 12-14hr		2558
embryo 14-16hr		2881
embryo 16-18hr		2428
embryo 18-20hr		2985
embryo 20-22hr		2824
embryo 22-24hr		2498
larva L1		3619
larva L2		2990
larva L3 12hr old		2362
larva L3 puffstage 1-2		1991
larva L3 puffstage 3-6		1819
larva L3 puffstage 7-9		1954
white prepupae new		1608
white prepupae 12hr		1501
white prepupae 24hr		1397
pupae 2d postWPP		1230
pupae 3d postWPP		1047
pupae 4d postWPP		1265
adult male 01day		935
adult male 05day		979
adult male 30day		967
adult female 01day		2256
adult female 05day		2075
adult female 30day		2277
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		2062
embryo 02-04hr		2812
embryo 04-06hr		3114
embryo 06-08hr		3199
embryo 08-10hr		3880
embryo 10-12hr		4672
embryo 12-14hr		2558
embryo 14-16hr		2881
embryo 16-18hr		2428
embryo 18-20hr		2985
embryo 20-22hr		2824
embryo 22-24hr		2498
larva L1		3619
larva L2		2990
larva L3 12hr old		2362
larva L3 puffstage 1-2		1991
larva L3 puffstage 3-6		1819
larva L3 puffstage 7-9		1954
white prepupae new		1608
white prepupae 12hr		1501
white prepupae 24hr		1397
pupae 2d postWPP		1230
pupae 3d postWPP		1047
pupae 4d postWPP		1265
adult male 01day		935
adult male 05day		979
adult male 30day		967
adult female 01day		2256
adult female 05day		2075
adult female 30day		2277
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(2062)
embryo 02-04hr		(2812)
embryo 04-06hr		(3114)
embryo 06-08hr		(3199)
embryo 08-10hr		(3880)
embryo 10-12hr		(4672)
embryo 12-14hr		(2558)
embryo 14-16hr		(2881)
embryo 16-18hr		(2428)
embryo 18-20hr		(2985)
embryo 20-22hr		(2824)
embryo 22-24hr		(2498)
larva L1		(3619)
larva L2		(2990)
larva L3 12hr old		(2362)
larva L3 puffstage 1-2		(1991)
larva L3 puffstage 3-6		(1819)
larva L3 puffstage 7-9		(1954)
white prepupae new		(1608)
white prepupae 12hr		(1501)
white prepupae 24hr		(1397)
pupae 2d postWPP		(1230)
pupae 3d postWPP		(1047)
pupae 4d postWPP		(1265)
adult male 01day		(935)
adult male 05day		(979)
adult male 30day		(967)
adult female 01day		(2256)
adult female 05day		(2075)
adult female 30day		(2277)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		(2062)
embryo 02-04hr		(2812)
embryo 04-06hr		(3114)
embryo 06-08hr		(3199)
embryo 08-10hr		(3880)
embryo 10-12hr		(4672)
embryo 12-14hr		(2558)
embryo 14-16hr		(2881)
embryo 16-18hr		(2428)
embryo 18-20hr		(2985)
embryo 20-22hr		(2824)
embryo 22-24hr		(2498)
larva L1		(3619)
larva L2		(2990)
larva L3 12hr old		(2362)
larva L3 puffstage 1-2		(1991)
larva L3 puffstage 3-6		(1819)
larva L3 puffstage 7-9		(1954)
white prepupae new		(1608)
white prepupae 12hr		(1501)
white prepupae 24hr		(1397)
pupae 2d postWPP		(1230)
pupae 3d postWPP		(1047)
pupae 4d postWPP		(1265)
adult male 01day		(935)
adult male 05day		(979)
adult male 30day		(967)
adult female 01day		(2256)
adult female 05day		(2075)
adult female 30day		(2277)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		2062
embryo 02-04hr		2812
embryo 04-06hr		3114
embryo 06-08hr		3199
embryo 08-10hr		3880
embryo 10-12hr		4672
embryo 12-14hr		2558
embryo 14-16hr		2881
embryo 16-18hr		2428
embryo 18-20hr		2985
embryo 20-22hr		2824
embryo 22-24hr		2498
larva L1		3619
larva L2		2990
larva L3 12hr old		2362
larva L3 puffstage 1-2		1991
larva L3 puffstage 3-6		1819
larva L3 puffstage 7-9		1954
white prepupae new		1608
white prepupae 12hr		1501
white prepupae 24hr		1397
pupae 2d postWPP		1230
pupae 3d postWPP		1047
pupae 4d postWPP		1265
adult male 01day		935
adult male 05day		979
adult male 30day		967
adult female 01day		2256
adult female 05day		2075
adult female 30day		2277
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		3187
Schneider line 2 Sg4		2409
embryonic 1182-4H		2527
embryonic GM2		937
embryonic Kc167		874
embryonic S1		1835
embryonic S3		1519
leg disc CME L1		2960
wing disc CME-W2		1321
wing disc ML-DmD8		2525
wing disc ML-DmD9		2791
wing disc ML-DmD16-c3		1648
wing disc ML-DmD21		6764
wing disc ML-DmD32		1947
haltere disc ML-DmD17-c3		1468
eye-antennal disc ML-DmD11		3231
antennal disc ML-DmD20-c5		3449
mixed discs ML-DmD4-c1		2782
CNS ML-DmBG1-c1		2038
CNS ML-DmBG2-c2		1541
tumorous blood cells mbn2		1486
ovary fGS/OSS		1847
ovary OSC		938
ovary OSS		566
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(3187)
Schneider line 2 Sg4		(2409)
embryonic 1182-4H		(2527)
embryonic GM2		(937)
embryonic Kc167		(874)
embryonic S1		(1835)
embryonic S3		(1519)
leg disc CME L1		(2960)
wing disc CME-W2		(1321)
wing disc ML-DmD8		(2525)
wing disc ML-DmD9		(2791)
wing disc ML-DmD16-c3		(1648)
wing disc ML-DmD21		(6764)
wing disc ML-DmD32		(1947)
haltere disc ML-DmD17-c3		(1468)
eye-antennal disc ML-DmD11		(3231)
antennal disc ML-DmD20-c5		(3449)
mixed discs ML-DmD4-c1		(2782)
CNS ML-DmBG1-c1		(2038)
CNS ML-DmBG2-c2		(1541)
tumorous blood cells mbn2		(1486)
ovary fGS/OSS		(1847)
ovary OSC		(938)
ovary OSS		(566)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(3187)
Schneider line 2 Sg4		(2409)
embryonic 1182-4H		(2527)
embryonic GM2		(937)
embryonic Kc167		(874)
embryonic S1		(1835)
embryonic S3		(1519)
leg disc CME L1		(2960)
wing disc CME-W2		(1321)
wing disc ML-DmD8		(2525)
wing disc ML-DmD9		(2791)
wing disc ML-DmD16-c3		(1648)
wing disc ML-DmD21		(6764)
wing disc ML-DmD32		(1947)
haltere disc ML-DmD17-c3		(1468)
eye-antennal disc ML-DmD11		(3231)
antennal disc ML-DmD20-c5		(3449)
mixed discs ML-DmD4-c1		(2782)
CNS ML-DmBG1-c1		(2038)
CNS ML-DmBG2-c2		(1541)
tumorous blood cells mbn2		(1486)
ovary fGS/OSS		(1847)
ovary OSC		(938)
ovary OSS		(566)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		3187
Schneider line 2 Sg4		2409
embryonic 1182-4H		2527
embryonic GM2		937
embryonic Kc167		874
embryonic S1		1835
embryonic S3		1519
leg disc CME L1		2960
wing disc CME-W2		1321
wing disc ML-DmD8		2525
wing disc ML-DmD9		2791
wing disc ML-DmD16-c3		1648
wing disc ML-DmD21		6764
wing disc ML-DmD32		1947
haltere disc ML-DmD17-c3		1468
eye-antennal disc ML-DmD11		3231
antennal disc ML-DmD20-c5		3449
mixed discs ML-DmD4-c1		2782
CNS ML-DmBG1-c1		2038
CNS ML-DmBG2-c2		1541
tumorous blood cells mbn2		1486
ovary fGS/OSS		1847
ovary OSC		938
ovary OSS		566
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		3187
Schneider line 2 Sg4		2409
embryonic 1182-4H		2527
embryonic GM2		937
embryonic Kc167		874
embryonic S1		1835
embryonic S3		1519
leg disc CME L1		2960
wing disc CME-W2		1321
wing disc ML-DmD8		2525
wing disc ML-DmD9		2791
wing disc ML-DmD16-c3		1648
wing disc ML-DmD21		6764
wing disc ML-DmD32		1947
haltere disc ML-DmD17-c3		1468
eye-antennal disc ML-DmD11		3231
antennal disc ML-DmD20-c5		3449
mixed discs ML-DmD4-c1		2782
CNS ML-DmBG1-c1		2038
CNS ML-DmBG2-c2		1541
tumorous blood cells mbn2		1486
ovary fGS/OSS		1847
ovary OSC		938
ovary OSS		566
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(3187)
Schneider line 2 Sg4		(2409)
embryonic 1182-4H		(2527)
embryonic GM2		(937)
embryonic Kc167		(874)
embryonic S1		(1835)
embryonic S3		(1519)
leg disc CME L1		(2960)
wing disc CME-W2		(1321)
wing disc ML-DmD8		(2525)
wing disc ML-DmD9		(2791)
wing disc ML-DmD16-c3		(1648)
wing disc ML-DmD21		(6764)
wing disc ML-DmD32		(1947)
haltere disc ML-DmD17-c3		(1468)
eye-antennal disc ML-DmD11		(3231)
antennal disc ML-DmD20-c5		(3449)
mixed discs ML-DmD4-c1		(2782)
CNS ML-DmBG1-c1		(2038)
CNS ML-DmBG2-c2		(1541)
tumorous blood cells mbn2		(1486)
ovary fGS/OSS		(1847)
ovary OSC		(938)
ovary OSS		(566)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		(3187)
Schneider line 2 Sg4		(2409)
embryonic 1182-4H		(2527)
embryonic GM2		(937)
embryonic Kc167		(874)
embryonic S1		(1835)
embryonic S3		(1519)
leg disc CME L1		(2960)
wing disc CME-W2		(1321)
wing disc ML-DmD8		(2525)
wing disc ML-DmD9		(2791)
wing disc ML-DmD16-c3		(1648)
wing disc ML-DmD21		(6764)
wing disc ML-DmD32		(1947)
haltere disc ML-DmD17-c3		(1468)
eye-antennal disc ML-DmD11		(3231)
antennal disc ML-DmD20-c5		(3449)
mixed discs ML-DmD4-c1		(2782)
CNS ML-DmBG1-c1		(2038)
CNS ML-DmBG2-c2		(1541)
tumorous blood cells mbn2		(1486)
ovary fGS/OSS		(1847)
ovary OSC		(938)
ovary OSS		(566)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		3187
Schneider line 2 Sg4		2409
embryonic 1182-4H		2527
embryonic GM2		937
embryonic Kc167		874
embryonic S1		1835
embryonic S3		1519
leg disc CME L1		2960
wing disc CME-W2		1321
wing disc ML-DmD8		2525
wing disc ML-DmD9		2791
wing disc ML-DmD16-c3		1648
wing disc ML-DmD21		6764
wing disc ML-DmD32		1947
haltere disc ML-DmD17-c3		1468
eye-antennal disc ML-DmD11		3231
antennal disc ML-DmD20-c5		3449
mixed discs ML-DmD4-c1		2782
CNS ML-DmBG1-c1		2038
CNS ML-DmBG2-c2		1541
tumorous blood cells mbn2		1486
ovary fGS/OSS		1847
ovary OSC		938
ovary OSS		566
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		954
cold shock, 4-day adult		721
heat shock, 4-day adult		1278
Cadmium 50 mM 6 hrs, larvae L3		1931
Cadmium 50 mM 12 hrs, larvae L3		1551
Cadmium 50 mM 48 hrs, 4-day adult		1033
Cadmium 100 mM 48 hrs, 4-day adult		1190
Copper 0.5 mM 12 hrs, larvae L3		1311
Copper 15 mM 48 hrs, 4-day adult		303
Zinc 5 mM 12 hrs, larvae L3		817
Zinc 4.5 mM 48 hrs, 4-day adult		471
Ethanol 2.5% 3 hrs, larvae L3		1764
Ethanol 5% 3 hrs, larvae L3		1652
Ethanol 10% 3 hrs, larvae L3		1738
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1604
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1348
Caffeine 25 mg/ml 48 hrs, 4-day adult		697
Paraquat 5 mM 48 hrs, 4-day adult		1460
Paraquat 10 mM 48 hrs, 4-day adult		1607
Rotenone 2 μg 12 hrs, larvae L3		1713
Rotenone 8 μg 12 hrs, larvae L3		2343
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(954)
cold shock, 4-day adult		(721)
heat shock, 4-day adult		(1278)
Cadmium 50 mM 6 hrs, larvae L3		(1931)
Cadmium 50 mM 12 hrs, larvae L3		(1551)
Cadmium 50 mM 48 hrs, 4-day adult		(1033)
Cadmium 100 mM 48 hrs, 4-day adult		(1190)
Copper 0.5 mM 12 hrs, larvae L3		(1311)
Copper 15 mM 48 hrs, 4-day adult		(303)
Zinc 5 mM 12 hrs, larvae L3		(817)
Zinc 4.5 mM 48 hrs, 4-day adult		(471)
Ethanol 2.5% 3 hrs, larvae L3		(1764)
Ethanol 5% 3 hrs, larvae L3		(1652)
Ethanol 10% 3 hrs, larvae L3		(1738)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1604)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(1348)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(697)
Paraquat 5 mM 48 hrs, 4-day adult		(1460)
Paraquat 10 mM 48 hrs, 4-day adult		(1607)
Rotenone 2 μg 12 hrs, larvae L3		(1713)
Rotenone 8 μg 12 hrs, larvae L3		(2343)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		(954)
cold shock, 4-day adult		(721)
heat shock, 4-day adult		(1278)
Cadmium 50 mM 6 hrs, larvae L3		(1931)
Cadmium 50 mM 12 hrs, larvae L3		(1551)
Cadmium 50 mM 48 hrs, 4-day adult		(1033)
Cadmium 100 mM 48 hrs, 4-day adult		(1190)
Copper 0.5 mM 12 hrs, larvae L3		(1311)
Copper 15 mM 48 hrs, 4-day adult		(303)
Zinc 5 mM 12 hrs, larvae L3		(817)
Zinc 4.5 mM 48 hrs, 4-day adult		(471)
Ethanol 2.5% 3 hrs, larvae L3		(1764)
Ethanol 5% 3 hrs, larvae L3		(1652)
Ethanol 10% 3 hrs, larvae L3		(1738)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1604)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(1348)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(697)
Paraquat 5 mM 48 hrs, 4-day adult		(1460)
Paraquat 10 mM 48 hrs, 4-day adult		(1607)
Rotenone 2 μg 12 hrs, larvae L3		(1713)
Rotenone 8 μg 12 hrs, larvae L3		(2343)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		954
cold shock, 4-day adult		721
heat shock, 4-day adult		1278
Cadmium 50 mM 6 hrs, larvae L3		1931
Cadmium 50 mM 12 hrs, larvae L3		1551
Cadmium 50 mM 48 hrs, 4-day adult		1033
Cadmium 100 mM 48 hrs, 4-day adult		1190
Copper 0.5 mM 12 hrs, larvae L3		1311
Copper 15 mM 48 hrs, 4-day adult		303
Zinc 5 mM 12 hrs, larvae L3		817
Zinc 4.5 mM 48 hrs, 4-day adult		471
Ethanol 2.5% 3 hrs, larvae L3		1764
Ethanol 5% 3 hrs, larvae L3		1652
Ethanol 10% 3 hrs, larvae L3		1738
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1604
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1348
Caffeine 25 mg/ml 48 hrs, 4-day adult		697
Paraquat 5 mM 48 hrs, 4-day adult		1460
Paraquat 10 mM 48 hrs, 4-day adult		1607
Rotenone 2 μg 12 hrs, larvae L3		1713
Rotenone 8 μg 12 hrs, larvae L3		2343
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		954
cold shock, 4-day adult		721
heat shock, 4-day adult		1278
Cadmium 50 mM 6 hrs, larvae L3		1931
Cadmium 50 mM 12 hrs, larvae L3		1551
Cadmium 50 mM 48 hrs, 4-day adult		1033
Cadmium 100 mM 48 hrs, 4-day adult		1190
Copper 0.5 mM 12 hrs, larvae L3		1311
Copper 15 mM 48 hrs, 4-day adult		303
Zinc 5 mM 12 hrs, larvae L3		817
Zinc 4.5 mM 48 hrs, 4-day adult		471
Ethanol 2.5% 3 hrs, larvae L3		1764
Ethanol 5% 3 hrs, larvae L3		1652
Ethanol 10% 3 hrs, larvae L3		1738
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1604
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1348
Caffeine 25 mg/ml 48 hrs, 4-day adult		697
Paraquat 5 mM 48 hrs, 4-day adult		1460
Paraquat 10 mM 48 hrs, 4-day adult		1607
Rotenone 2 μg 12 hrs, larvae L3		1713
Rotenone 8 μg 12 hrs, larvae L3		2343
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(954)
cold shock, 4-day adult		(721)
heat shock, 4-day adult		(1278)
Cadmium 50 mM 6 hrs, larvae L3		(1931)
Cadmium 50 mM 12 hrs, larvae L3		(1551)
Cadmium 50 mM 48 hrs, 4-day adult		(1033)
Cadmium 100 mM 48 hrs, 4-day adult		(1190)
Copper 0.5 mM 12 hrs, larvae L3		(1311)
Copper 15 mM 48 hrs, 4-day adult		(303)
Zinc 5 mM 12 hrs, larvae L3		(817)
Zinc 4.5 mM 48 hrs, 4-day adult		(471)
Ethanol 2.5% 3 hrs, larvae L3		(1764)
Ethanol 5% 3 hrs, larvae L3		(1652)
Ethanol 10% 3 hrs, larvae L3		(1738)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1604)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(1348)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(697)
Paraquat 5 mM 48 hrs, 4-day adult		(1460)
Paraquat 10 mM 48 hrs, 4-day adult		(1607)
Rotenone 2 μg 12 hrs, larvae L3		(1713)
Rotenone 8 μg 12 hrs, larvae L3		(2343)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		(954)
cold shock, 4-day adult		(721)
heat shock, 4-day adult		(1278)
Cadmium 50 mM 6 hrs, larvae L3		(1931)
Cadmium 50 mM 12 hrs, larvae L3		(1551)
Cadmium 50 mM 48 hrs, 4-day adult		(1033)
Cadmium 100 mM 48 hrs, 4-day adult		(1190)
Copper 0.5 mM 12 hrs, larvae L3		(1311)
Copper 15 mM 48 hrs, 4-day adult		(303)
Zinc 5 mM 12 hrs, larvae L3		(817)
Zinc 4.5 mM 48 hrs, 4-day adult		(471)
Ethanol 2.5% 3 hrs, larvae L3		(1764)
Ethanol 5% 3 hrs, larvae L3		(1652)
Ethanol 10% 3 hrs, larvae L3		(1738)
Caffeine 1.5 mg/ml 4 hrs, larvae L3		(1604)
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(1348)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(697)
Paraquat 5 mM 48 hrs, 4-day adult		(1460)
Paraquat 10 mM 48 hrs, 4-day adult		(1607)
Rotenone 2 μg 12 hrs, larvae L3		(1713)
Rotenone 8 μg 12 hrs, larvae L3		(2343)
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		954
cold shock, 4-day adult		721
heat shock, 4-day adult		1278
Cadmium 50 mM 6 hrs, larvae L3		1931
Cadmium 50 mM 12 hrs, larvae L3		1551
Cadmium 50 mM 48 hrs, 4-day adult		1033
Cadmium 100 mM 48 hrs, 4-day adult		1190
Copper 0.5 mM 12 hrs, larvae L3		1311
Copper 15 mM 48 hrs, 4-day adult		303
Zinc 5 mM 12 hrs, larvae L3		817
Zinc 4.5 mM 48 hrs, 4-day adult		471
Ethanol 2.5% 3 hrs, larvae L3		1764
Ethanol 5% 3 hrs, larvae L3		1652
Ethanol 10% 3 hrs, larvae L3		1738
Caffeine 1.5 mg/ml 4 hrs, larvae L3		1604
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		1348
Caffeine 25 mg/ml 48 hrs, 4-day adult		697
Paraquat 5 mM 48 hrs, 4-day adult		1460
Paraquat 10 mM 48 hrs, 4-day adult		1607
Rotenone 2 μg 12 hrs, larvae L3		1713
Rotenone 8 μg 12 hrs, larvae L3		2343
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0017579

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG6253-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

RpL14^GD13508

lethal | adult stage, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

lethal | adult stage, with Scer\GAL4^ey.PH

RpL14^{dsRNA.IR.Scer\UAS}

lethal | embryonic stage, with Scer\GAL4^Act5C.PI

RpL14^{dsRNA.IR.Scer\UAS}

lethal | embryonic stage | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

lethal | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

lethal | larval stage | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

lethal | pupal stage, with Scer\GAL4^Dll-md23

RpL14^{dsRNA.IR.Scer\UAS}

lethal | pupal stage, with Scer\GAL4^Tab2-201Y

RpL14^{dsRNA.IR.Scer\UAS}

lethal | recessive

RpL14¹

viable, with Scer\GAL4^toy3

RpL14^{dsRNA.IR.Scer\UAS}

viable, with Scer\GAL4^toy4

RpL14^{dsRNA.IR.Scer\UAS}

viable, with Scer\GAL4^toy5

RpL14^{dsRNA.IR.Scer\UAS}

viable, with Scer\GAL4^toy6

RpL14^{dsRNA.IR.Scer\UAS}

Other Phenotypes

Allele

cell growth defective | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PP

RpL14^Scer\UAS.cMa

cell growth defective | somatic clone, with Scer\GAL4^Act5C.PP

RpL14^Scer\UAS.cMa

Minute

RpL14¹

Minute | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

visible, with Scer\GAL4^bbg-C96

RpL14^{dsRNA.IR.Scer\UAS}

visible, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

visible, with Scer\GAL4^drl-PGAL8

RpL14^{dsRNA.IR.Scer\UAS}

visible, with Scer\GAL4^ey.PH

RpL14^{dsRNA.IR.Scer\UAS}

visible, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

visible | dominant

RpL14^unspecified

Phenotype manifest in

Allele

adult head, with Scer\GAL4^ey.PH

RpL14^{dsRNA.IR.Scer\UAS}

anterior scutellar bristle | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

arista, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

chaeta

RpL14¹

chaeta | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

cornea, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

embryonic/larval salivary gland, with Scer\GAL4^AB1

RpL14^{dsRNA.IR.Scer\UAS}

embryonic/larval salivary gland, with Scer\GAL4^ey.PH

RpL14^{dsRNA.IR.Scer\UAS}

eye, with Scer\GAL4^drl-PGAL8

RpL14^{dsRNA.IR.Scer\UAS}

eye, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

eye-antennal disc, with Scer\GAL4^ey.PH

RpL14^{dsRNA.IR.Scer\UAS}

interommatidial bristle, with Scer\GAL4^drl-PGAL8

RpL14^{dsRNA.IR.Scer\UAS}

interommatidial bristle, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

leg, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

macrochaeta

microchaeta, with Scer\GAL4^bbg-C96

RpL14^{dsRNA.IR.Scer\UAS}

ommatidium, with Scer\GAL4^drl-PGAL8

RpL14^{dsRNA.IR.Scer\UAS}

ommatidium, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

pigment cell, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

posterior dorsocentral bristle | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

posterior scutellar bristle | heat sensitive, with Scer\GAL4^hs.PB

RpL14^{dsRNA.IR.Scer\UAS}

postvertical bristle, with Scer\GAL4^drl-PGAL8

RpL14^{dsRNA.IR.Scer\UAS}

pseudocone, with Scer\GAL4^GMR.PF

RpL14^{dsRNA.IR.Scer\UAS}

scutellar bristle

RpL14¹

scutellum, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

thoracico-abdominal ganglion | pharate adult stage, with Scer\GAL4^ey.PH

RpL14^{dsRNA.IR.Scer\UAS}

wing

RpL14¹

wing, with Scer\GAL4^bbg-C96

RpL14^{dsRNA.IR.Scer\UAS}

wing, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

wing disc

RpL14¹

wing vein, with Scer\GAL4^bbg-C96

RpL14^{dsRNA.IR.Scer\UAS}

wing vein, with Scer\GAL4^dpp.blk1

RpL14^{dsRNA.IR.Scer\UAS}

Classical Alleles ( 4 )

For All Classical Alleles Show

Allele of RpL14	Mutagen	Stocks	Known lesion
RpL14¹	P-element activity	2	Yes
RpL14^MB11564		1	--
RpL14^1rv1	Delta2-3	0	--
RpL14^unspecified		0	--

Alleles Carried on Transgenic Constructs ( 5 )

For All Alleles Carried on Transgenic Constructs Show

Allele of RpL14	Mutagen	Stocks	Known lesion
RpL14^GD13508	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
RpL14^KK109141	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
RpL14^{dsRNA.IR.Scer\UAS}	in vitro construct - regulatory fusion in vitro construct - RNAi	0	Yes
RpL14^NIG.6253R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
RpL14^Scer\UAS.cMa	in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Not disrupted in

Df(3L)66C-G28

(Saeboe-Larssen et al., 1997)

Df(3L)h-i22

(Saeboe-Larssen et al., 1997)

Df(3L)pbl-X1

(Saeboe-Larssen et al., 1997)

Df(3L)ZN47

(Saeboe-Larssen et al., 1997)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}RpL14¹

P{lacW}RpL14^1rv1

insertion of enhancer trap binary system

Mi{ET1}RpL14^MB11564

Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )

Terms Based on Experimental Evidence ( 7 terms )

Molecular Function

CV term

Evidence

References

structural constituent of ribosome

inferred from direct assay

(Alonso and Santaren, 2006)

Biological Process

CV term

Evidence

References

centrosome duplication