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Gene Dmel\lok

General Information
Symbol	Dmel\lok	Species	D. melanogaster
Name	loki	Annotation symbol	CG10895
Feature type	protein_coding_gene	FlyBase ID	FBgn0019686
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	chk2, mnk, DmCHk2, Dmnk, 38B.4
Genomic Location
Chromosome (arm)	2L	Recombination map	2-
Cytogenetic map	38B2-38B2	Sequence location	2L:20,061,477..20,064,381 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene loki is referred to in FlyBase by the symbol Dmel\lok (CG10895, FBgn0019686). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: protein kinase activity; protein serine/threonine kinase activity. There is experimental evidence that it is involved in the biological process: apoptotic process; activation of caspase activity; germ cell programmed cell death; response to DNA damage stimulus; germ cell development; DNA damage response, signal transduction resulting in induction of apoptosis; DNA damage checkpoint. 14 alleles are reported. The phenotype of these alleles is annotated with: egg chamber. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Forkhead-associated (FHA) domain; Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; SMAD/FHA domain; Serine/threonine-protein kinase, active site; Serine/threonine-protein kinase, catalytic domain; Serine/threonine-protein kinase-like domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1625038_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of lok. Gene sequence location is 2L:20061477..20064381.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2012_01	Sequence features BKN51203 BKN20909 HFA03567 BKN50122 References Khurana et al., 2010, PLoS Genet. 6(12): e1001246 Rouget et al., 2010, Nature 467(7319): 1128--1132 Lancaster et al., 2010, PLoS Genet. 6(10): e1001179
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 38B2-38B2   Limits computationally determined from genome sequence between P{lacW}barrk14014 and P{EP}nebEP642
Experimentally Determined Cytological Location
Cytogenetic map Notes References 38B-38B     (Minami, 1997.10.2) 38B-38B     (Larochelle, 1997.1.31) 38B-38B   (determined by in situ hybridisation)   (Oishi et al., 1998) 38B-38B   (determined by in situ hybridisation)   (Hijal, 1997.4.17)
Experimentally Determined Recombination Data
Location	2- (Larochelle, 1997.1.31)
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\lok for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) lok-RA FBtr0081328  NM_057871   2176   476 lok-RB FBtr0081329  NM_057870   2125   459 lok-RC FBtr0081330  NM_165318   2183   459 lok-RD FBtr0306234     2330   284
Additional Transcript Data & Comments
Reported size (kB)	2.2 (northern blot) (Oishi et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein lok-PA   FBpp0080860   54.3   476   9.54     NP_477219   AAF53867 lok-PB   FBpp0080861   52.3   459   9.39     NP_477218   AAN11062 lok-PC   FBpp0080862   52.3   459   9.39     NP_724241   AAN11063 lok-PD   FBpp0297344   32.5   284   9.64
Additional Polypeptide Data & Comments
Reported size (kDa)	476, 459 (aa); 56, 54 (kD predicted) (Oishi et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (FHA) domain (IPR000253) Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) SMAD/FHA domain (IPR008984) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA816409   AA949096   AA951171   AE014134 AAF53867   AE014134 AAN11062   AE014134 AAN11063   AB007821 BAA28755   AB007822 BAA28756   AC004364   AC009252   AI532068   AW942781   AY070549 AAL48020   BI164599   BI170187   BI214954   BI239814   BI241615   BI356197   BI357201   BI357257   BI578275   BI632532   BI639501   BI639590   BI640595   BP541694   BP556041   BP556042   BP558648   BP558649   BT120173   BT120181   CK136045   CO299280   CO304724   CO325538   EC051973   EC054478   EC054979   EC056308   EC200985   EC204441   EC226111   EC248698   EV575540   EV588541   EV591739   EV592683   EV594463   EV594875   EV596495   EV596786   EV598057   EV598512   EV599396   EV600114   FE863461   FE863483   FE863557   FE863805   FE863869   FE863942   FE863978   GH746608   GH761807   GH762440   GH762548   GH763361   GH765887   U87984 AAB49642
UniProtKB/Swiss-Prot	O61267
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 2 -- 5   organism | anterior   (Lecuyer et al., 2007) Comment:diffuse anterior enrichment northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage | early     (Oishi et al., 1998) embryonic stage 1 -- 2   organism | ubiquitous   (Oishi et al., 1998) embryonic stage 3 -- 17   pole cell   (Oishi et al., 1998) oogenesis & adult stage | female   ovary   (Oishi et al., 1998) adult stageoogenesis stage S2 -- S14   nurse cell   (Oishi et al., 1998)
Additional Descriptive Data
	lok expression in early embryos is diffusely concentrated at the anterior end of the embryo. (Lecuyer et al., 2007) lok transcripts are detected strongly in ovaries and in early embryos on northern blots. lok transcripts are detected from very early in oogenesis. They are first observed in region 2 of the germarium in a patchy pattern and in region 3 are localized to a single spot which may be the oocyte. From stage S2, strong staining is observed in the oocyte and weaker expression is seen in the cytoplasm of the nurse cells. By stage S8, the staining is localized to the anterior edge of the oocyte. In early cleavage stage embryos, lok transcri ts are strongly expressed throughout the embryo with a slight anterior-posterior gradient. By stage 3, expression throughout the embryo diminishes but strong staining remains in the pole cells. Expression in the pole cells is sustained during blastoderm and gastrulation stages. Both lok transcripts are expressed in ovaries and embryos. The ratio of the short form to the long form is ~100:1 in ovaries and 1~5:1 in late embryos. (Oishi et al., 1998)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 3 -- 4   organism | ubiquitous   (Oishi et al., 1998)
Additional Descriptive Data
	lok protein is first detected in somatic and germ line cells at stage 3. By the cellular blastoderm stage, protein is no longer observed in the somatic cells. Expression in the pole cells is sustained through gastrulation and is weakly detectable after the pole cells have migrated into the abdominal cavity. (Oishi et al., 1998)
Marker for
Subcellular Localization
CV Term	nucleus (Oishi et al., 1998)
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0019686    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0019686 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0019686 expression level Developmental Stage   Expression Level embryo 00-02hr    16578 embryo 02-04hr    5542 embryo 04-06hr    4826 embryo 06-08hr    3032 embryo 08-10hr    2585 embryo 10-12hr    1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day    4814 adult female 05day    11140 adult female 30day    11203 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (16578) embryo 02-04hr  (5542) embryo 04-06hr  (4826) embryo 06-08hr  (3032) embryo 08-10hr  (2585) embryo 10-12hr  (1544) embryo 12-14hr  (1033) embryo 14-16hr  (716) embryo 16-18hr  (626) embryo 18-20hr  (482) embryo 20-22hr  (399) embryo 22-24hr  (611) larva L1  (370) larva L2  (275) larva L3 12hr old  (286) larva L3 puffstage 1-2  (469) larva L3 puffstage 3-6  (557) larva L3 puffstage 7-9  (696) white prepupae new  (596) white prepupae 12hr  (1003) white prepupae 24hr  (906) pupae 2d postWPP  (425) pupae 3d postWPP  (244) pupae 4d postWPP  (292) adult male 01day  (167) adult male 05day  (187) adult male 30day  (233) adult female 01day  (4814) adult female 05day  (11140) adult female 30day  (11203) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (16578) embryo 02-04hr  (5542) embryo 04-06hr  (4826) embryo 06-08hr  (3032) embryo 08-10hr  (2585) embryo 10-12hr  (1544) embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day  (4814) adult female 05day  (11140) adult female 30day  (11203) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (16578) embryo 02-04hr    5542 embryo 04-06hr    4826 embryo 06-08hr    3032 embryo 08-10hr    2585 embryo 10-12hr    1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day    4814 adult female 05day  (11140) adult female 30day  (11203) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    16578 embryo 02-04hr    5542 embryo 04-06hr    4826 embryo 06-08hr    3032 embryo 08-10hr    2585 embryo 10-12hr    1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day    4814 adult female 05day    11140 adult female 30day    11203 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0019686 expression level Developmental Stage   Expression Level embryo 00-02hr    16578 embryo 02-04hr    5542 embryo 04-06hr    4826 embryo 06-08hr    3032 embryo 08-10hr    2585 embryo 10-12hr    1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day    4814 adult female 05day    11140 adult female 30day    11203 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (16578) embryo 02-04hr  (5542) embryo 04-06hr  (4826) embryo 06-08hr  (3032) embryo 08-10hr  (2585) embryo 10-12hr  (1544) embryo 12-14hr  (1033) embryo 14-16hr  (716) embryo 16-18hr  (626) embryo 18-20hr  (482) embryo 20-22hr  (399) embryo 22-24hr  (611) larva L1  (370) larva L2  (275) larva L3 12hr old  (286) larva L3 puffstage 1-2  (469) larva L3 puffstage 3-6  (557) larva L3 puffstage 7-9  (696) white prepupae new  (596) white prepupae 12hr  (1003) white prepupae 24hr  (906) pupae 2d postWPP  (425) pupae 3d postWPP  (244) pupae 4d postWPP  (292) adult male 01day  (167) adult male 05day  (187) adult male 30day  (233) adult female 01day  (4814) adult female 05day  (11140) adult female 30day  (11203) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (16578) embryo 02-04hr  (5542) embryo 04-06hr  (4826) embryo 06-08hr  (3032) embryo 08-10hr  (2585) embryo 10-12hr  1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day  (4814) adult female 05day  (11140) adult female 30day  (11203) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  16578 embryo 02-04hr    5542 embryo 04-06hr    4826 embryo 06-08hr    3032 embryo 08-10hr    2585 embryo 10-12hr    1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day    4814 adult female 05day  11140 adult female 30day  11203 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    16578 embryo 02-04hr    5542 embryo 04-06hr    4826 embryo 06-08hr    3032 embryo 08-10hr    2585 embryo 10-12hr    1544 embryo 12-14hr    1033 embryo 14-16hr    716 embryo 16-18hr    626 embryo 18-20hr    482 embryo 20-22hr    399 embryo 22-24hr    611 larva L1    370 larva L2    275 larva L3 12hr old    286 larva L3 puffstage 1-2    469 larva L3 puffstage 3-6    557 larva L3 puffstage 7-9    696 white prepupae new    596 white prepupae 12hr    1003 white prepupae 24hr    906 pupae 2d postWPP    425 pupae 3d postWPP    244 pupae 4d postWPP    292 adult male 01day    167 adult male 05day    187 adult male 30day    233 adult female 01day    4814 adult female 05day    11140 adult female 30day    11203 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0019686    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0019686 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0019686 expression level Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary    1511 Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary  (1511) Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary  (1511) Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary    1511 Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0019686 expression level Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary    1511 Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary  (1511) Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary  1511 Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    128.275 Larval Midgut    40.8 Larval Hindgut    53.5 Larval Malpighian Tubules    48.4 Larval Fat Body    29.7 Larval Salivary Gland    35.9 Larval Trachea    97 Larval Carcass    46.875 Adult Head    33.9 Adult Eye    22.175 Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop    76.1 Adult Midgut    47.2 Adult Hindgut    52.5 Adult Malpighian Tubules    66.5 Adult Fat Body    32 Adult Salivary Gland    40.2 Adult Heart    20.275 Adult VirginFemale Spermatheca    33.6 Adult InseminatedFemale Spermatheca    24.4 Adult Ovary    1511 Adult Testis    44.7 Adult Male Accessory Gland    52.7 Adult Carcass    59.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain no informative data Adult Thoracic-Abdominal Ganglion no informative data Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    33.9   NA  Eye    22.175   NA  Brain    no informative data   128.275  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    76.1   40.8  Midgut    47.2   53.5  Hindgut    52.5   48.4  Malpighian Tubules    66.5   29.7  Fat Body    32   35.9  Salivary Gland    40.2   NA  Heart    20.275   97  Trachea    NA   NA  VirginFemale Spermatheca    33.6   NA  InseminatedFemale Spermatheca    24.4   NA  Ovary    1511   NA  Testis    44.7   NA  Male Accessory Gland    52.7   46.875  Carcass    59.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0019686 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10895-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for lok Stages(s) 4-6 Stages(s) 7-8 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | maternal effect lokE51 viable, with Scer\GAL4pnr-MD237 lokGD4190 Other Phenotypes Allele chemical resistant lokp6 decreased cell death lokp6 increased cell death, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 increased cell death, with Scer\GAL4nos.UTR.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 radiation sensitive lokp6 radiation sensitive | recessive lokE51 wild-type lokhs.PX Phenotype manifest in Allele egg chamber, with Scer\GAL4nos.UTR.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 egg chamber | oogenesis stage S8, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 egg chamber | oogenesis stage S8, with Scer\GAL4nos.UTR.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 egg chamber | oogenesis stage S9, with Scer\GAL4mat.αTub67C.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 egg chamber | oogenesis stage S9, with Scer\GAL4nos.UTR.T:Hsim\VP16 lokScer\UAS.P\T.T:Ivir\HA1 mitosis & nuclear chromosome & embryo lokE51 nucleus | embryonic stage lokE51 spindle | embryonic stage lokE51
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of lok Class Mutagen Stocks Known lesion lok30 P-element activity 0 Yes lokE51 Delta2-3 0 Yes lokp6 loss of function allele P-element activity 0 Yes lokunspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of lok Class Mutagen Stocks Known lesion lokGD4190 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes lokGL00020 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes lok+tBa in vitro construct - genomic fragment 0 Yes lok3'.tMa in vitro construct - genomic fragment 0 Yes lok5'.tMa in vitro construct - genomic fragment 0 Yes lokD303A.Scer\UAS in vitro construct - amino acid replacement 0 Yes lokhs.PX in vitro construct - regulatory fusion 0 Yes lokKK100813 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes lokScer\UAS.cPa in vitro construct - regulatory fusion 0 Yes lokScer\UAS.P\T.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)E51 (Xu et al., 2001) Df(2L)Exel7077 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4190} NA P{KK100813} NA P{TRiP.GL00020} NA P{UAS-lok.D303A} NA P{UAS-lok.P} NA P{UASp-lok.HA} NA heat-shock construct P{hs-lok.X} NA characterization construct P{barr+tMa} NA P{CG10728+tMa} NA P{mnk-rescued} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{}lokp6 NA
Gene Ontology: Function, Process & Cellular Component ( 14 unique terms )
Terms Based on Experimental Evidence ( 8 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
activation of caspase activity inferred from mutant phenotype (Akdemir et al., 2007) apoptotic process inferred from mutant phenotype (Wichmann et al., 2006) DNA damage checkpoint inferred from direct assay (Sakurai et al., 2011) inferred from mutant phenotype (Xu et al., 2001, LaRocque et al., 2007) DNA damage response, signal transduction resulting in induction of apoptosis inferred from mutant phenotype (Xu et al., 2001) germ cell development inferred from expression pattern (assigned by UniProtKB) (Oishi et al., 1998) germ cell programmed cell death inferred from mutant phenotype (Bakhrat et al., 2010) response to DNA damage stimulus inferred from mutant phenotype (Takada et al., 2003, Ravi et al., 2009)
Cellular Component
CV term Evidence References
nucleus inferred from direct assay (assigned by UniProtKB) (Oishi et al., 1998)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity inferred from sequence or structural similarity with human CHEK2 (Sekelsky et al., 2000) protein serine/threonine kinase activity inferred from electronic annotation with InterPro:IPR008271 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (assigned by UniProtKB) (Oishi et al., 1998) non-traceable author statement (Hijal et al., 1997, Larochelle, 1997.1.31, Morrison et al., 2000)
Biological Process
CV term Evidence References
cell cycle checkpoint inferred from sequence or structural similarity with human CHEK2 (Sekelsky et al., 2000) traceable author statement (Lee and Orr-Weaver, 2003) protein phosphorylation inferred from sequence or structural similarity (assigned by UniProtKB) (Oishi et al., 1998) inferred from sequence or structural similarity with human CHEK2 (Sekelsky et al., 2000) non-traceable author statement (Morrison et al., 2000)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Hijal et al., 1997)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions lok allele Gene References lokD303A.Scer\UAS p53 (Peters et al., 2002) lokE51 polo (Xu and Du, 2003) stg (Xu and Du, 2003) lokp6 grp (Takada et al., 2003) spn-D (Abdu et al., 2002) wee (Stumpff et al., 2004) lokScer\UAS.cPa p53 (Peters et al., 2002) unspecified armi (Klattenhoff et al., 2007) aub (Klattenhoff et al., 2007) mus301 (McCaffrey et al., 2006) spn-A (Staeva-Vieira et al., 2003) spn-D (Klattenhoff et al., 2007) wee (Stumpff et al., 2005)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 61259 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0019686 DroID - A comprehensive database of gene and protein interactions. • FBgn0019686 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35288
Orthologs
Genome-wide drosophilid orthologs	Dana\GF14445 Dere\GG21217 Dgri\GH13656 Dmoj\GI17408 Dper\GL26345 Dpse\GA10622 Dsec\GM17387 Dsim\GD24241 Dvir\GJ18340 Dwil\GK18729 Dyak\GE13292
Curated drosophilid orthologs	Dpse\GA10622
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL007544-PA Aedes aegypti (mosquito) AGAP009784-PA Anopheles gambiae (mosquito) XP_624334.2 Apis mellifera (honey bee) XP_001951526.1 Acyrthosiphon pisum (pea aphid) At2g30360.1 Arabidopsis thaliana (wall cress) 85591 Batrachochytrium dendrobatidis (chytrid fungus) 230456 Branchiostoma floridae (lancelet) BGIBMGA005370-PA Bombyx mori (silk worm) ENSBTAP00000006518 Bos taurus (bovine) CAL0004640 Candida albicans (a form of yeast) CE28251 Caenorhabditis elegans (worm) ENSCAFP00000017544 Canis familiaris (dog) CAGL0M02233g Candida glabrata (haploid yeast) ENSCINP00000010261 Ciona intestinalis (sea squirt) JA06063 Caenorhabditis japonica (nematode) CPIJ009147 Culex pipens (mosquito) ENSCPOP00000005566 Cavia porcellus (guinea pig) RP28023 Caenorhabditis remanei (nematode) ENSCSAVP00000008401 Ciona savignyi (sedentary tunicate) 224883 Capitella spl (polychaete worm) DEHA2G21142g Debaryomyces Hansenii (yeast) 300413 Daphnia pulex (daphnia) ENSDARP00000036490 Danio rerio (zebra fish) ENSECAP00000004610 Equus caballus (horse) XP_655060 Entamoeba histolytica (anaerobic protozoan) ENSGALP00000009122 Gallus gallus (chicken) ENSP00000372023 Homo sapiens (human) KLLA0E01585g Kluyveromyces lactis (yeast) 231530 Lottia gigantea (owl limpet) LmjF07.0900 Leishmania major (virus) 15828 Monocosta brevicollis (choanoflagellate) ENSMODP00000010421 Monodelphis domestica (gray opossum) ENSMMUP00000037143 Macaca mulatta (rhesus monkey) ENSMUSP00000066679 Mus musculus (house mouse) 163148 Nematostella vectensis (starlet anemone) XP_001601897.1 Nasonia vitripennis (wasp) ENSOANP00000014054 Ornithorynchus anatinus (platypus) ENSORLP00000024979 Oryzias latipes (japanese killifish) LOC_Os09g25100.1 Oryza sativa (asian rice) PHUM002689-PA Pediculus humanus (human body louse) PP26313 Pristionchus pacificus (nematode) 143594 Physcomitrella patens (moss) 221343 Physcomitrella patens (moss) ENSPPYP00000013022 Pongo pygmaeus (orangutan) ENSPTRP00000049753 Pan troglodytes (chimpanzee) ENSRNOP00000053643 Rattus norvegicus (norway rat) YDL101C Saccharomyces cerevisiae (budding yeast) Smp_171370 Schistosoma mansoni (trematode parasite) SPCC18B5.11c Schizosaccharomyces pombe (fission yeast) XP_001183431.1 Strongylocentrotus purpuratus (purple sea urchin) 29114 Trichoplax adhaerens (metazoa) XP_972529.1 Tribolium castaneum (red flour beetle) Tc00.1047053511277.140 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000007060 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000035808 Takifugu rubripes (pufferfish Takifugu) 83973.m00063 Trichomonas vaginalis (parasitic flagellated protozoan) 84270.m00563 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000046701 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13234 NV12140 LH13520 FBgn0230978 FBgn0220727 FBgn0205499 FBgn0195589 FBgn0172297 JGI_V11_300413 JGI_V11_333806 FBgn0070678 FBgn0163927 FBgn0140152 FBgn0121132 FBgn0113397 FBgn0091472 CPIJ016532 CPIJ009147 BGIBMGA005370 ACYPI004596 GB14096 AGAP009784 AGAP008341 AAEL007544 PHUM016570 GLEAN_15484
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	35152 y1 sc* v1; P{TRiP.GL00020}attP2/TM3, Sb1
VDRC	v44980 w1118; P{GD4190}v44980 v44981 w1118; P{GD4190}v44981
Genomic Clones ( 2 )
	BACR10H13 BACR22E16 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 59 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD27857
Other clones	DPiM_LD27857 MIP15302 MIP15402
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD01443 LD31503 RE04305 RE11565 RE21120 RE36409 RE38730 RE42285 RE43616 RE43681 RE72126 SD03432 SD22219 SD23521 SD26335
Other clones	69750 117459 122715 173956 Dmel_dig_005830_0683_3640 Dmel_neb_005883_0826_0688 Dmel_neb_006358_0896_2535 Dmel_neb_007012_0885_1895 Dmel_neb_008853_0131_1258 Dmel_neb_009689_0826_2431 Dmel_neb_009802_0591_0509 Dmel_neb_011255_0584_1267 Dmel_neb_011285_0837_1576 Dmel_neb_012259_0699_2988 Dmel_neb_013465_0344_2876 Dmel_neb_015649_0827_1472 EC051973 EC054478 EC054979 EC056308 EK175001 EK196738 EK245916 FGC004D08 FGM301A11 FGM316C07 RM07493 RM07693 RM07793 RM07893 RM07993 RM08093 RM08193 RP001008754 RP001015502 RP001017519 RP001041134 RP001048272 RP001053395 SD22085
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0019686 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35288
Antibody Information
	polyclonal (Oishi et al., 1998)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: lok CG10895 (Burtis, 1999.11)
Source for database merge of
Additional comments	"lok" may be allelic to "vls". (Oishi et al., 1998)
Other Comments
	S2 cells are treated with dsRNA made from templates generated with primers directed against this gene to assess effect on proteolysis of dup and geminin genes. (Higa et al., 2003) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) lok appears to be a transducer of the meiotic checkpoint that is activated by unrepaired double-strand DNA breaks. (Abdu et al., 2002) Mutants show defects in maintaining genome stability and are highly sensitive to ionizing radiation. Mutation in lok completely blocks DNA damage-induced apoptosis and partially blocks damage-induced cell cycle arrest. (Xu et al., 2001) Identification and developmental expression of lok are reported, a nuclear protein Ser/Thr kinase. The unique spatio-temporal expression pattern and structural features of lok indicate it is a novel class of nuclear protein kinases. (Oishi et al., 1998) lok encodes a serine/threonine protein kinase. (Hijal, 1997.4.17)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057870 NM_057871 NM_165318 RefSeq (Proteins) • NP_477218 NP_477219 NP_724241 DDBJ / EMBL / GenBank • AA949096 AAB49642 AAF53867 AAL48020 AAN11062 AAN11063 AB007821 AB007822 AC004364 AC009252 AW942781 AY070549 BAA28755 BAA28756 BI639501 U87984 Entrez Gene - A searchable database of RefSeq genes. • 35288 UniProtKB/Swiss-Prot • O61267
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Forkhead-associated (FHA) domain (IPR000253) Protein kinase, catalytic domain (IPR000719) Serine/threonine-protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) SMAD/FHA domain (IPR008984) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) Serine/threonine-protein kinase-like domain (IPR017442)
Linkouts
	BioGRID - A database of protein and genetic interactions • 61259 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0019686 DroID - A comprehensive database of gene and protein interactions. • FBgn0019686 DRSC - Results from RNAi screens. • FBgn0019686 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0019686 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10895-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0019686 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 35288 InParanoid A subset of ortholog calls from InParanoid. • AAEL007544-PA Aedes aegypti (mosquito) AGAP009784-PA Anopheles gambiae (mosquito) XP_624334.2 Apis mellifera (honey bee) XP_001951526.1 Acyrthosiphon pisum (pea aphid) At2g30360.1 Arabidopsis thaliana (wall cress) 85591 Batrachochytrium dendrobatidis (chytrid fungus) 230456 Branchiostoma floridae (lancelet) BGIBMGA005370-PA Bombyx mori (silk worm) ENSBTAP00000006518 Bos taurus (bovine) CAL0004640 Candida albicans (a form of yeast) CE28251 Caenorhabditis elegans (worm) ENSCAFP00000017544 Canis familiaris (dog) CAGL0M02233g Candida glabrata (haploid yeast) ENSCINP00000010261 Ciona intestinalis (sea squirt) JA06063 Caenorhabditis japonica (nematode) CPIJ009147 Culex pipens (mosquito) ENSCPOP00000005566 Cavia porcellus (guinea pig) RP28023 Caenorhabditis remanei (nematode) ENSCSAVP00000008401 Ciona savignyi (sedentary tunicate) 224883 Capitella spl (polychaete worm) DEHA2G21142g Debaryomyces Hansenii (yeast) 300413 Daphnia pulex (daphnia) ENSDARP00000036490 Danio rerio (zebra fish) ENSECAP00000004610 Equus caballus (horse) XP_655060 Entamoeba histolytica (anaerobic protozoan) ENSGALP00000009122 Gallus gallus (chicken) ENSP00000372023 Homo sapiens (human) KLLA0E01585g Kluyveromyces lactis (yeast) 231530 Lottia gigantea (owl limpet) LmjF07.0900 Leishmania major (virus) 15828 Monocosta brevicollis (choanoflagellate) ENSMODP00000010421 Monodelphis domestica (gray opossum) ENSMMUP00000037143 Macaca mulatta (rhesus monkey) ENSMUSP00000066679 Mus musculus (house mouse) 163148 Nematostella vectensis (starlet anemone) XP_001601897.1 Nasonia vitripennis (wasp) ENSOANP00000014054 Ornithorynchus anatinus (platypus) ENSORLP00000024979 Oryzias latipes (japanese killifish) LOC_Os09g25100.1 Oryza sativa (asian rice) PHUM002689-PA Pediculus humanus (human body louse) PP26313 Pristionchus pacificus (nematode) 143594 Physcomitrella patens (moss) 221343 Physcomitrella patens (moss) ENSPPYP00000013022 Pongo pygmaeus (orangutan) ENSPTRP00000049753 Pan troglodytes (chimpanzee) ENSRNOP00000053643 Rattus norvegicus (norway rat) YDL101C Saccharomyces cerevisiae (budding yeast) Smp_171370 Schistosoma mansoni (trematode parasite) SPCC18B5.11c Schizosaccharomyces pombe (fission yeast) XP_001183431.1 Strongylocentrotus purpuratus (purple sea urchin) 29114 Trichoplax adhaerens (metazoa) XP_972529.1 Tribolium castaneum (red flour beetle) Tc00.1047053511277.140 Trypanosoma cruzi (parasitic trypanosome) ENSTNIP00000007060 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000035808 Takifugu rubripes (pufferfish Takifugu) 83973.m00063 Trichomonas vaginalis (parasitic flagellated protozoan) 84270.m00563 Trichomonas vaginalis (parasitic flagellated protozoan) ENSXETP00000046701 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /newgene/chk2-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 35288 modMine - Data generated by the modENCODE project. • FBgn0019686 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB13234 NV12140 LH13520 FBgn0230978 FBgn0220727 FBgn0205499 FBgn0195589 FBgn0172297 JGI_V11_300413 JGI_V11_333806 FBgn0070678 FBgn0163927 FBgn0140152 FBgn0121132 FBgn0113397 FBgn0091472 CPIJ016532 CPIJ009147 BGIBMGA005370 ACYPI004596 GB14096 AGAP009784 AGAP008341 AAEL007544 PHUM016570 GLEAN_15484
Synonyms & Secondary IDs ( 27 )
Reported As
Symbol Synonym	38B.4 (McGill Drosophila Genome Project, 2001.9.25, Butler, 2001, Butler et al., 2001) 10895 (Ravi et al., 2009) CG10895 (Morrison et al., 2000, Keleman et al., 2009.8.5, Ni et al., 2011.7.19) chk (Staeva-Vieira et al., 2003) CHK-2 (Schupbach et al., 2005) chk2 (Stumpff et al., 2005, Huynh and St. Johnston, 2004, Masrouha et al., 2003, Gur-Arie and Abdu, 2007, Wichmann et al., 2006, Wells et al., 2006, Garcia et al., 2007, Baehrecke, 2006, Akdemir et al., 2007, Klovstad et al., 2008, Pane et al., 2007, Chen et al., 2007, Mehrotra et al., 2008, Chen et al., 2007, Peretz et al., 2009, Abdu et al., 2007, Lu et al., 2010) CHK2 (Higa et al., 2003, Katzenberger et al., 2006, Yamasaki, 2007, Merkle et al., 2009) Chk2 (Sutcliffe and Brehm, 2004, Raff, 2003, Sibon, 2003, Klattenhoff et al., 2007, Rickmyre et al., 2007, Merkle et al., 2007, Wichmann et al., 2007, Hochwagen, 2006, Takada et al., 2007, Rickmyre et al., 2007, Benoit et al., 2009, Klattenhoff et al., 2009, Iijima-Ando et al., 2010, Iijima et al., 2010, Sakurai et al., 2011, Rouget et al., 2010) chk2/lok (Cavey et al., 2005) DmCHk2 (Lee and Orr-Weaver, 2003) Dmchk2 (Xu and Du, 2003, Abdu et al., 2002, Xu et al., 2001) DmChk2 (Peters et al., 2002, Takada et al., 2003, LaRocque et al., 2007, Bakhrat et al., 2010) Dmnk (Mahowald, 2001, Iwai et al., 2002, Oishi et al., 1998) Dmnk/DChk2 (de Vries et al., 2005) Dmnk-L (Minami, 1997.10.2) Dmnk-S (Minami, 1997.10.2) lok (Mukai et al., 2006, LaRocque et al., 2007, Blanco et al., 2010, LaRocque et al., 2007, Lecuyer et al., 2007, Titen and Golic, 2008, Peng and Karpen, 2009, Maezawa et al., 2009, Dworkin et al., 2009, Obbard et al., 2009, Terashima and Bownes, 2005) loki (Merkle et al., 2009) LOKI/CHK2 (Morrison et al., 2000) mnk (Stumpff et al., 2004, Brodsky, 2003.5.16, Takada et al., 2003, McCaffrey et al., 2006, Rickmyre et al., 2007, Merkle et al., 2007, Klattenhoff et al., 2007, Wells et al., 2006, Bi et al., 2005, Takada et al., 2007, Merkle and Lee, 2008, Brodsky et al., 2004, Oikemus et al., 2006, Rickmyre et al., 2007, Blumenstiel et al., 2008, Merkle et al., 2009, Benoit et al., 2009, Klattenhoff et al., 2009, Orsi et al., 2010, Becalska et al., 2011, Sakurai et al., 2011, Lancaster et al., 2010, Khurana et al., 2010, Rouget et al., 2010) MNK (Lee and Orr-Weaver, 2003) unnamed (Hijal et al., 1997)
Name Synonym	Checkpoint kinase 2 (Merkle and Lee, 2008, Iijima-Ando et al., 2010, Iijima et al., 2010) checkpoint kinase 2 (Katzenberger et al., 2006, Benoit et al., 2009) Drosophila maternal nuclear kinase (Oishi et al., 1998) localized ovarian kinase (Hijal, 1997.4.17) loki (Larochelle, 1997.1.31, Mukai et al., 2006, Tadros et al., 2007, Mehrotra et al., 2008, Rickmyre et al., 2007, Maezawa et al., 2009, Terashima and Bownes, 2005) maternal nuclear kinase (Sekelsky et al., 2000, Rickmyre et al., 2007, Merkle et al., 2009, Sakurai et al., 2011)
Secondary FlyBase IDs
References ( 104 )
Generate a list of	All references relating to this gene ( 104 )
List References by type	Research paper  ( 63 ) Supplementary material  ( 6 ) Review  ( 8 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 9 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 3 ) Conference report  ( 1 ) Curated genome annotation  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 12 )
	Becalska et al., 2011, Dev. Biol. 349(1): 46--52 Aubergine is a component of a nanos mRNA localization complex. [FBrf0212348] Sakurai et al., 2011, FEBS Lett. 585(12): 1923--1928 Anaphase DNA bridges induced by lack of RecQ5 in Drosophila syncytial embryos. [FBrf0213874] Bakhrat et al., 2010, Apoptosis 15(12): 1425--1434 Drosophila Chk2 and p53 proteins induce stage-specific cell death independently during oogenesis. [FBrf0212376] Blanco et al., 2010, BMC Dev. Biol. 10: 94 Gene expression following induction of regeneration in Drosophila wing imaginal discs. Expression profile of regenerating wing discs. [FBrf0211812] Iijima-Ando et al., 2010, Hum. Mol. Genet. 19(10): 1930--1938 A DNA damage-activated checkpoint kinase phosphorylates tau and enhances tau-induced neurodegeneration. [FBrf0210684] Iijima et al., 2010, Hum. Mol. Genet. 19(15): 2947--2957 Tau Ser262 phosphorylation is critical for A{beta}42-induced tau toxicity in a transgenic Drosophila model of Alzheimer's disease. [FBrf0211234] Khurana et al., 2010, PLoS Genet. 6(12): e1001246 Distinct Functions for the Drosophila piRNA Pathway in Genome Maintenance and Telomere Protection. [FBrf0212661] Lancaster et al., 2010, PLoS Genet. 6(10): e1001179 The Meiotic Recombination Checkpoint Suppresses NHK-1 Kinase to Prevent Reorganisation of the Oocyte Nucleus in Drosophila. [FBrf0212208] Lu et al., 2010, Science 328(5983): 1278--1281 Meiotic recombination provokes functional activation of the p53 regulatory network. [FBrf0210965] Orsi et al., 2010, J. Cell Sci. 123(20): 3515--3524 Drosophila I-R hybrid dysgenesis is associated with catastrophic meiosis and abnormal zygote formation. [FBrf0212012] Papaconstantinou et al., 2010, PLoS ONE 5(11): e14049 Menin Links the Stress Response to Genome Stability in Drosophila melanogaster. [FBrf0212463] Rouget et al., 2010, Nature 467(7319): 1128--1132 Maternal mRNA deadenylation and decay by the piRNA pathway in the early Drosophila embryo. [FBrf0212183] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...