Ice, caspase-3
a effector caspase - activated during spermatogenesis and necessary for the removal of cytoplasm and the generation of functional sperm
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Gene model reviewed during 5.49
1.02 (longest cDNA)
There is only one protein coding transcript and one polypeptide associated with this gene
339 (aa)
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 21 kDa (p21) and a 12 kDa (p12) subunit. Inactive pro-form can homodimerize. Dronc and Drice can form a stable complex (PubMed:10675329). Interacts with Diap2 (via BIR3 domain) to form a stable complex (PubMed:18166655).
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Comment: maternally deposited
The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.
Drice transcript is expressed in all developmental stages, with highest levels detected in 2-6 hr embryos.
Comment: reference states 12-14h APF
Comment: reference states 12-14h APF
An antibody thatspecifically recognizes the activated form of Drice was used to examine expression of this protein during apoptosis that occurs in nurse cells during oogenesis. Foci of activated Drice staining was first observed near nuclei of the nurse cells closest to the oocyte. Later, at stage 12, increased expression of Drice was observed in all nurse cells.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Drice in GBrowse 2
3-99
3-99
3-97.0
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
polyclonal antibody CM1
Source for identity of: Drice Ice
Changed from 'Ice' to 'Drice' to reflect common usage in the literature.
Ice is activated in wing discs after irradiation.
th blocks effector caspases by targeting them for polyubiquitylation and nonproteasomal inactivation.
Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
A CED-4-like P-loop-containing ATPase is involved in the activation of Ice to promote the cell death program in Drosophila.
Overexpression of Ice sensitizes cells to apoptotic stimuli and expression of an N-terminally truncated form rapidly induces apoptosis. Ice is a Cys protease that cleaves baculovirus p35 and Lam in vitro and Ice is expressed at all stages of development at which programmed cell death can be induced. These results strongly suggest that Ice is an apoptotic caspase that acts downstream of rpr.
Ice encodes an essential caspase required for apoptotic activity in Drosophila cells.
