Gene model reviewed during 5.49
There is only one protein coding transcript and one polypeptide associated with this gene
Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 21 kDa (p21) and a 12 kDa (p12) subunit. Inactive pro-form can homodimerize. Dronc and Drice can form a stable complex (PubMed:10675329). Interacts with Diap2 (via BIR3 domain) to form a stable complex (PubMed:18166655).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Drice using the Feature Mapper tool.
The expression of this gene along with several others is induced in salivary glands in pupae at the time of head eversion. This stage is characterized by an increase in the ecdysone titer as well as large amounts of cell death in this tissue.
Processing of the full-length Drice precursor transcript (35 kDa) differs between pupal and larval salivary gland and Malpighian tubule tissue. Only the pupal salivary gland shows a band of fully activated Drice at 19 kDa.
Drice protein detected by an antibody specific to the activated form is observed in the cytoplasm of salivary glands beginning at 10 hours APF. Levels increase with time, and activated Drice protein is abundant at 14 hours APF.
Active Drice protein is observed to accumulate in the cytoplasm of preindividualized part of the spermatid cyst, and in the cystic bulge, but is not detectable in the postindividualized part of the spermatid cyst. Inactive Drice protein is observed uniformly in preindividualizing spermatid cysts.
An antibody thatspecifically recognizes the activated form of Drice was used to examine expression of this protein during apoptosis that occurs in nurse cells during oogenesis. Foci of activated Drice staining was first observed near nuclei of the nurse cells closest to the oocyte. Later, at stage 12, increased expression of Drice was observed in all nurse cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Drice in GBrowse 2
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Drice Ice
th blocks effector caspases by targeting them for polyubiquitylation and nonproteasomal inactivation.
Gene expression is increased in response to the presence of two copies of Scer\GAL4hs.PB.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
A CED-4-like P-loop-containing ATPase is involved in the activation of Ice to promote the cell death program in Drosophila.
Overexpression of Ice sensitizes cells to apoptotic stimuli and expression of an N-terminally truncated form rapidly induces apoptosis. Ice is a Cys protease that cleaves baculovirus p35 and Lam in vitro and Ice is expressed at all stages of development at which programmed cell death can be induced. These results strongly suggest that Ice is an apoptotic caspase that acts downstream of rpr.