dGCN2
protein kinase that promotes avoidance of the essential amino acid-deficient diet - blocks translation initiation through eIF2a phosphorylation - required for infection-induced host translational blockage
Please see the JBrowse view of Dmel\Gcn2 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.47
6.0 (northern blot)
6.5 (northern blot)
1589 (aa)
1589 (aa); 175 (kD observed); 179 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Gcn2 using the Feature Mapper tool.
Comment: maternally deposited
Gcn2 transcripts are detected throughout development and are particularly abundant in early embryos.
Gcn2 transcripts are expressed throughout development as revealed by northern blots with peaks in 0-12hr embryos, third instar larvae, and adults. High levels of Gcn2 transcripts are first detected by in situ hybridization in stages 2 and 3 prior to cellularization. In late stage 4 and stage 5, transcripts are concentrated in the cortex. By gastrulation, expression is concentrated at areas of cell movement, including all the furrows and the proctodeal primordium. During germ band extension, expression occurs in primordia of the anterior and posterior midguts and in the mesoderm. By the end of stage 12, transcripts accumulate in the anterior and posterior midgut and in areas of the primitive brain and the CNS. By stage 13, Gcn2 transcripts are observed throughout the CNS including the supraesophageal ganglion and the ventral nerve cord. In later stages, the CNS expression is restricted to 4 cells per neuromere which are probably neurons.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Gcn2 in GBrowse 23-102
3-99.1
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Gcn2 CG1609
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, spindle abnormalities, chromosome abnormalities and chromosome alignment defects seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The presence of Gcn2 in Drosophila suggests at least partial conservation from yeast to multicellular organisms of the mechanisms responding to amino acid deprivation.
