Gene Dmel\Fab-7

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General Information

Symbol

Dmel\Fab-7

Species

D. melanogaster

Name

Fab-7

Annotation symbol

Feature type

insulator

FlyBase ID

FBgn0020011

Gene Model Status

Unannotated

Stock availability

1 publicly available

Also Known As

Fab7

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

89E2-89E3

Sequence location

Summary Information

Automatically generated summary

See sections below for more information

The gene Fab-7 is referred to in FlyBase by the symbol Dmel\Fab-7 (FBgn0020011). It is a insulator from Drosophila melanogaster. Its molecular function is unknown. The biological processes in which it is involved are not known. 32 alleles are reported. No phenotypic data is available. It has no annotated transcripts. Gene has not been localized to the genome sequence.

User Contributed Data

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Recent Updates

Description

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This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

89E2-89E3

Left limit from (method unavailable) (FBrf0091909) Right limit from (method unavailable) (FBrf0091909)

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

89E2-89E3

(Mishra et al., 1997)

Experimentally Determined Recombination Data

Location

(Mishra et al., 1997)

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Mapped Features

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

modENCODE Anatomy RNA-Seq

modENCODE Development RNA-Seq

modENCODE Cell Lines RNA-Seq

modENCODE Treatments RNA-Seq

Expression Clusters

External Data & Images

Linkouts

Alleles & Phenotypes

Summary of Allele Phenotypes

Phenotype manifest in

Allele

Classical Alleles ( 3 )

For All Classical Alleles Show

Allele of Fab-7	Class	Mutagen	Stocks	Known lesion
Fab-7¹			0	Yes
Fab-7²			0	Yes
Fab-7³¹⁶			0	Yes

Alleles Carried on Transgenic Constructs ( 29 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Fab-7	Mutagen	Stocks	Known lesion
Fab-7^3.35	in vitro construct	1	Yes
Fab-7^0.86	in vitro construct	0	Yes
Fab-7^0.8	in vitro construct	0	Yes
Fab-7^1.2.m1-2	in vitro construct - site directed mutagenesis	0	Yes
Fab-7^1.2.m1-5	in vitro construct - site directed mutagenesis	0	Yes
Fab-7^1.2.m3-4	in vitro construct - site directed mutagenesis	0	Yes
Fab-7^1.2	in vitro construct	0	Yes
Fab-7^1.7	in vitro construct - deletion	0	Yes
Fab-7^3.7	in vitro construct	0	Yes
Fab-7^Ab	in vitro construct	0	Yes
Fab-7^GA.m	in vitro construct - site directed mutagenesis	0	Yes
Fab-7^GAF.m	in vitro construct - site directed mutagenesis	0	Yes
Fab-7^HA	in vitro construct - deletion	0	Yes
Fab-7^HN	in vitro construct - deletion	0	Yes
Fab-7^Midi	in vitro construct	0	Yes
Fab-7^Mini	in vitro construct	0	Yes
Fab-7^NE	in vitro construct - deletion	0	Yes
Fab-7^PA	in vitro construct - deletion	0	Yes
Fab-7^PHO.m	in vitro construct - site directed mutagenesis	0	Yes
Fab-7^{Scer\FRT.Act5C.FLA}	cre recombinase	0	Yes
Fab-7^{Scer\FRT.Act5C.FTA}	cre recombinase	0	Yes
Fab-7^{Scer\FRT.P1\loxP.Act5C.FAas}	in vitro construct	0	Yes
Fab-7^{Scer\FRT.P1\loxP.Act5C.FAs}	in vitro construct	0	Yes
Fab-7^{Scer\FRT.P1\loxP.Act5C.lambda.FLA}	in vitro construct - regulatory fusion	0	Yes
Fab-7^{Scer\FRT.P1\loxP.Act5C.txterm.FTA}	in vitro construct - regulatory fusion	0	Yes
Fab-7^{Scer\FRT.P1\loxP.hb.FHas}	in vitro construct	0	Yes
Fab-7^{Scer\FRT.P1\loxP.hb.FHs}	in vitro construct	0	Yes
Fab-7^ΔGA	in vitro construct - deletion	0	Yes
Fab-7^ΔZ	in vitro construct - site directed mutagenesis	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

characterization construct

P{2xPE-Fab-7-IAB5}

P{2xPE-spacer-IAB5}

P{(FRT.Fab-7)(loxP.Act5C)FAas}

P{(FRT.Fab-7)(loxP.Act5C)FAs}

P{(FRT.Fab-7)(loxP.hb)FHas}

P{(FRT.Fab-7)(loxP.hb)FHs}

P{(FRT.Fab-7)(-loxP)FAas}

P{(FRT.Fab-7)(-loxP)FAs}

P{(FRT.Fab-7)(-loxP)FHas}

P{(FRT.Fab-7)(-loxP)FHs}

P{(-FRT)(loxP.Act5C)FAas}

P{(-FRT)(loxP.Act5C)FAs}

P{(-FRT)(loxP.hb)FHas}

P{(-FRT)(loxP.hb)FHs}

P{(I-SceI.Eye).F7.YW.-172}

P{(I-SceI.Eye)(FRT.F7)Y(loxP.F7R)W.FR}

P{(I-SceI.Eye)(FRT.F7)Y(-loxP)W.FR}

P{(I-SceI.Eye)(FRT.F7R)Y(loxP.F7R)W.RR}

P{(I-SceI.Eye)(FRT.F7R)Y(-loxP)W.RR}

P{(I-SceI.Eye)(-FRT)Y(loxP.F7R)W.FR}

P{(I-SceI.Eye)(-FRT)Y(loxP.F7R)W.RR}

P{(I-SceI.Eye)(loxP.2.7)(FRT.F7)YW.2.7}

P{(I-SceI.Eye)(loxP.F7)YW.-343}

P{(I-SceI.Eye)(-loxP)(FRT.F7)YW.2.7}

P{(I-SceI.Eye)F7Y(loxP.F7R)(FRT.1.4)W.FR1.4}

P{(I-SceI.Eye)F7Y(loxP.F7R)(-FRT)W.FR1.4}

P{(I-SceI.Eye)F7Y(-loxP)(FRT.1.4)W.FR1.4}

P{(I-SceI.Eye)Y(loxP.F7)W.F}

P{(I-SceI.Eye)Y(loxP.F7R)(FRT.1.4)W.R1.4}

P{(I-SceI.Eye)Y(loxP.F7R)(-FRT)W.R1.4}

P{(-I-SceI).F7.YW.-172}

P{(-I-SceI)(FRT.F7)Y(loxP.F7R)W.FR}

P{(-I-SceI)(FRT.F7)Y(-loxP)W.FR}

P{(-I-SceI)(FRT.F7R)Y(loxP.F7R)W.RR}

P{(-I-SceI)(-FRT)Y(loxP.F7R)W.FR}

P{(-I-SceI)(loxP.2.7)(FRT.F7)YW.2.7}

P{(-I-SceI)(loxP.F7)YW.-343}

P{(-I-SceI)F7Y(loxP.F7R)(FRT.1.4)W.FR1.4}

P{(-I-SceI)Y(loxP.F7)W.F}

P{(-I-SceI)Y(loxP.F7R)(FRT.2.4)W.R1.4}

P{(loxP.F7)Eye(F7)YW}

P{AbGAF^m}

P{Act5C(loxP.txterm)(FRT.Fab-7)FTA}

P{Act5C(loxP.λ)(FRT.Fab-7)FLA}

P{Act5C(-loxP)(FRT.Fab-7)FLA}

P{Act5C(-loxP)(FRT.Fab-7)FTA}

P{Fab7-ff#2}

P{Fab7-fh#2}

P{Fab-7-lacZ.1.2.m1-2}

P{Fab-7-lacZ.1.2.m1-5}

P{Fab-7-lacZ.1.2.m3-4}

P{H1-spacer-Z-3'IAB5}

P{W267.Fab-8.Fab-7.iab-8}

P{W270.Fab-8.Fab-7.PTS6.iab-8}

P{w.GMR.y.a}

P{w.Scer\FRT.Fab-7.GMR.y}

P{w^RW}

P{w^RW+we}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 0 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 0 terms )

Sequence Ontology: Class of Gene

insulator

(Hagstrom et al., 1996, Zhou et al., 1996, Mishra et al., 1997)

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Fab-7 allele

Gene

References

Fab-7¹

(Bantignies et al., 2003)

unspecified

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0020011

Orthologs

OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

No orthologs identified

Dipteran inclusive ortholog search

No orthologs identified

Insect inclusive ortholog search

No orthologs identified

Arthropod inclusive ortholog search

No orthologs identified

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (None identified)

No orthologies identified

Orthologs in non-Drosophila Dipterans (None identified)

No non-Drosophilid orthologies identified

Orthologs in non-Dipteran Insects (None identified)

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (None identified)

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (0) based on analysis using Dmel annotation version 4.3

No orthologs identified

Stocks & Reagents

Stocks Listed in FlyBase ( 1 )

Bloomington

9418

w¹; P{Fab7-w#5}18.8.6

Genomic Clones ( 0 )

cDNA Clones ( 0 )

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

5657585

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Other Comments

Fab-7 and MCP-PRE appear to have two distinct roles; they regulate the expression of their flanking genes in cis, but they also mediate long-distance regulatory interactions with Hox genes in the Antennapedia complex.

(Bantignies et al., 2011)

Continuous transcription through the Fab-7 PRE in a transgene, producing either sense or antisense Fab-7 RNA, leads to the epigenetic activation of the Fab-7 element.

(Schmitt et al., 2005)

Trl protein-binding sites are necessary but not sufficient for full Fab-7 enhancer-blocking activity. Distinct Trl protein-binding sites within the Fab-7 element are required for different enhancer-blocking activities at different stages of development.

(Schweinsberg et al., 2004)

Fab-7 sites exhibit epigenetic inheritance of depressed chromatin states.

(Bantignies et al., 2003)

A Fab-7 element is switched from a silenced to a mitotically heritable active state by an embryonic pulse of transcription. Activated Fab-7 enables transcription of a gene even after withdrawal of the primary transcription factor.

(Cavalli, 1999)

Fab-7 can be switched to an active or a silencing mode. Both epigenetically determined states can be transmitted to a fraction of progeny in the next generation through the female germline. This suggests that a protein-based cellular memory mechanism can be propogated through meiosis.

(Cavalli and Paro, 1998)

iab-7 PRE.

(Hagstrom et al., 1997)

The Fab-7 region can be subdivided into a chromatin domain boundary and a Polycomb-response element.

(Mihaly et al., 1997)

Chromatin domain boundary element in the Bithorax-complex. Fab-7 can be functionally subdivided into enhancer block and Pc response elements.

(Mishra et al., 1997)

Deletion of the Fab-7 element results in fusion of the iab-6 and iab-7 cis-regulatory domains into a single regulatory domain that inappropriately regulates Abd-B. This result suggests Fab-7 is a chromatin domain boundary within the context of the bithorax complex that normally functions to ensure the autonomous activity of the iab-6 and iab-7 cis-regulatory domains.

(Hagstrom et al., 1996)

A specialised DNA element, Fab-7, is proposed to function as a boundary element that separates the iab-6 and iab-7 cis regulatory regions within the Abd-B domain of the BX-C. Studies suggest Fab-7 functions as an attenuator which weakens gene expression by reducing enhancer-promoter interactions. Fab-7 selectively blocks distal enhancers in an orientation-independent fashion and can function when far from either the distal enhancer or target promoter.

(Zhou et al., 1996)

The role of Fab-7 as a boundary element may be restricted to particular tissues in which the homeotic genes are active.

(Zink and Paro, 1995)

The Fab-7 element shows an orientation-dependent silencing of w^+mC but this silencing varies between different insertion lines.

(Zink and Paro, 1995)

The sequence and chromatin organisation of Fab-7 and Mcp have been compared.

(Karch et al., 1994)

The 3' flanking region of Abd-B includes three silencer regulatory regions, IAB5, MCP and Fab-7, whose function is dependent on segmentation gene products.

(Busturia and Bienz, 1993)

The expression of the P{bluetail} insertion into the PS12-specific regulatory domain in Abd-B^blt allows dissection of the neighboring cis-regulatory region into independent domains.

(Galloni et al., 1993)

Boundary elements in the bithorax complex, such as Fab-7 organize the parasegment specific cis-regulatory sub-regions into a series of autonomous domains, insulating each domain from the regulatory influences of the adjacent ones.

(Galloni et al., 1993)

An insulating boundary element in the 'Fab7' region is characterized by an unusual chromatin structure.

(Galloni et al., 1993)

Fab-7 and Mcp region chromatin structure contain distinct chromatin structures that display similarities to the scs and scs' structures of the Hsp70A locus, and are constitutive. Deletion analysis demonstrates that the DNA segment required for Fab-7 function contains 3 nuclease hypersensitive regions and that for Mcp function contains 1 major hypersensitive region and 3 minor nuclease hypersensitive regions.

(Vazquez et al., 1993)

External Crossreferences & Linkouts

Sequence Crossreferences

Other Crossreferences

Linkouts

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0020011

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

5657585

Synonyms & Secondary IDs ( 6 )

Reported As

Symbol Synonym

Fab7

(Schmitt et al., 2005, Schmitt et al., 2003, Hogga et al., 2001, Majumder and Cai, 2003, Cai et al., 2003, Schweinsberg and Schedl, 2000, Maurange et al., 1999, Schweinsberg et al., 1999, Paro et al., 1998, Li et al., 2006, Schwartz and Pirrotta, 2007, Sanchez-Elsner et al., 2006, Rank et al., 2002, Tariq et al., 2009, Chopra et al., 2009, Tchurikov et al., 2009, Li et al., 2008, Li et al., 2010)

FAB-7

(Zaret and Wolffe, 2001)

Fab-7 PRE

(Dejardin and Cavalli, 2002)

Fab7 PRE

(Lloyd, 2000)

Name Synonym

Fab-7

(Holohan et al., 2007)

Frontabdominal-7

(Pérez-Lluch et al., 2008, Lin et al., 2011)

Secondary FlyBase IDs

References ( 138 )

Generate a list of

All references relating to this gene ( 138 )

List References by type

Recent research papers ( 9 )

Bantignies et al., 2011, Cell 144(2): 214--226: Polycomb-Dependent Regulatory Contacts between Distant Hox Loci in Drosophila. [FBrf0212775]
Gohl et al., 2011, Genetics 187(3): 731--748: Mechanism of chromosomal boundary action: roadblock, sink, or loop? [FBrf0214438]
Kyrchanova et al., 2011, Nucleic Acids Res. 39(8): 3042--3052: Selective interactions of boundaries with upstream region of Abd-B promoter in Drosophila bithorax complex and role of dCTCF in this process. [FBrf0213528]
Kyrchanova et al., 2011, Russ. J. Genet. 47(12): 1406--1414: Interacting insulators from the Drosophila melanogaster bithorax complex can form independent expression domains. [FBrf0218791]
Kyrchanova et al., 2011, Genetika, Moscow 47(12): 1586--1595: [Interacting insulators from the Drosophila melanogaster bithorax complex can form independent expression domains]. [FBrf0217600]
Li et al., 2011, Mol. Cell. Biol. 31(4): 616--625: Insulators, Not Polycomb Response Elements, Are Required for Long-Range Interactions between Polycomb Targets in Drosophila melanogaster. [FBrf0212884]
Lin et al., 2011, Mol. Cell. Biol. 31(13): 2729--2741: A Barrier-Only Boundary Element Delimits the Formation of Facultative Heterochromatin in Drosophila melanogaster and Vertebrates. [FBrf0213867]
Okulski et al., 2011, Epigenetics Chromatin 4: 4: Quantitative analysis of polycomb response elements (PREs) at identical genomic locations distinguishes contributions of PRE sequence and genomic environment. [FBrf0214418]
Strübbe et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(14): 5572--5577: Polycomb purification by in vivo biotinylation tagging reveals cohesin and Trithorax group proteins as interaction partners. [FBrf0213360]

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Recent reviews (0)

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