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Gene Dmel\14-3-3ε

General Information
Symbol	Dmel\14-3-3ε	Species	D. melanogaster
Name	14-3-3ε	Annotation symbol	CG31196
Feature type	protein_coding_gene	FlyBase ID	FBgn0020238
Gene Model Status	Current	Stock availability	18 publicly available
Also Known As	14-3-3, D14-3-3ε, 14-3-3epsilon, 14-3-3e, par-5
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	90F10-90F10	Sequence location	3R:14,068,253..14,075,030 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene 14-3-3ε is referred to in FlyBase by the symbol Dmel\14-3-3ε (CG31196, FBgn0020238). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; protein heterodimerization activity. There is experimental evidence for 12 unique biological process terms, many of which group under: single-organism developmental process; biological regulation; cellular component organization or biogenesis; cell cycle; cell cycle process; regulation of growth; response to DNA damage stimulus; localization; response to UV; determination of adult lifespan. 39 alleles are reported. The phenotypes of these alleles are annotated with: posterior crossvein; eye; oocyte; pole cell; anterior crossvein; segmental nerve branch SNa of A1-7; eye photoreceptor cell; intersegmental nerve branch ISNb of A1-7; ommatidium; cycle 14 embryo. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: 14-3-3 domain; 14-3-3 protein; 14-3-3 protein, conserved site. Summary of modENCODE Temporal Expression Profile: Very high expression at all stages of development. Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628694_a_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of 14-3-3epsilon. Gene sequence location is 3R:14068253..14075030.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 90F10-90F10   Limits computationally determined from genome sequence between P{EP}cpoEP3679 and P{EP}Dlc90FEP3634
Experimentally Determined Cytological Location
Cytogenetic map Notes References 90F6-90F7     (Chang, 1997.1.10) 90F6-90F7   (determined by in situ hybridisation)   (Spradling et al., 1999) 90F6-90F7   (determined by in situ hybridisation)   (Perrimon et al., 1996) 90F6-90F7     (BDGP Project Members, 1994-1999) 90E-90F   On basis of meiotic mapping (details unspecified).   (Karim et al., 1996)
Experimentally Determined Recombination Data
Location	3- (Karim et al., 1996) 3-62.0 (Chang and Rubin, 1997)
Left of (cM)
Right of (cM)
Notes	Maps 0.4 +/- 0.2 cM from a w+-marked P-element insertion into 90E. (Dickson et al., 1996)
Gene Model & Products
Please see the GBrowse view of Dmel\14-3-3ε for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) 14-3-3ε-RA FBtr0083565  NM_169796   1239   262 14-3-3ε-RB FBtr0083566  NM_169797   2121   261 14-3-3ε-RC FBtr0083567  NM_169799   1221   256 14-3-3ε-RD FBtr0083568  NM_169798   2118   260
Additional Transcript Data & Comments
Reported size (kB)	1.453 (longest cDNA) (Chang and Rubin, 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein 14-3-3ε-PA   FBpp0082987   29.8   262   4.41     NP_732309   AAF55519 14-3-3ε-PB   FBpp0082988   29.7   261   4.44     NP_732310   AAN13764 14-3-3ε-PC   FBpp0082989   29.2   256   4.47     NP_732312   AAN13766 14-3-3ε-PD   FBpp0082990   29.6   260   4.44     NP_732311   AAN13765
Additional Polypeptide Data & Comments
Reported size (kDa)	260 (aa) (Chang and Rubin, 1997) 30 (kD observed) (Tien et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • 14-3-3 protein (IPR000308) 14-3-3 protein, conserved site (IPR023409) 14-3-3 domain (IPR023410)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA201167   AA201834   AA246750   AA246837   AA246879   AA264050   AA264084   AA264363   AA264792   AA264848   AA265011   AA391472   AA391477   AA391567   AA391719   AA438624   AA439654   AA440106   AA440333   AA440585   AA441151   AA441210   AA441340   AA540891   AA540913   AA540914   AA694906   AA695162   AA696243   AA697074   AA698187   AA802024   AA802885   AA816901   AA821183   AA940825   AA940953   AA941474   AA949119   AA950253   AA950571   AA951343   AA952302   AA979039   AA979363   AA979395   AA979468   AE014297 AAF55519   AE014297 AAN13764   AE014297 AAN13765   AE014297 AAN13766   AI061819   AI110135   AI257391   AI295917   AI297355   AI389112   AI512316   AI512688   AI515348   AI518092   AI519627   AI520001   AI520613   AI546556   AK254724   AK259646   AQ026302   AX260179   AX664478   BF486807   BF486907   BF487703   BF492082   BF492091   BF496544   BF501644   BG631989   BG636153   BG636787   BG639291   BG640237   BG640637   BG641090   BI162057   BI164938   BI164983   BI165167   BI165941   BI166581   BI168609   BI170052   BI170188   BI170794   BI170961   BI173486   BI212945   BI213118   BI213794   BI215841   BI216431   BI227493   BI229730   BI231602   BI235520   BI235645   BI235683   BI236332   BI236508   BI237195   BI237330   BI238743   BI238752   BI239502   BI240636   BI241230   BI241742   BI243194   BI243574   BI353946   BI354358   BI354376   BI356352   BI356445   BI358354   BI358731   BI361477   BI362061   BI362516   BI362800   BI367044   BI367646   BI367897   BI368426   BI371985   BI372004   BI372014   BI372077   BI372899   BI373212   BI373222   BI373657   BI373715   BI373973   BI375371   BI482588   BI483981   BI484281   BI484377   BI484796   BI486368   BI486946   BI563867   BI566344   BI567957   BI568052   BI570058   BI574690   BI576291   BI577708   BI578111   BI579763   BI580102   BI580322   BI580323   BI583090   BI583263   BI585385   BI587437   BI588235   BI590021   BI590823   BI604992   BI605852   BI609371   BI610692   BI614496   BI615294   BI617599   BI618171   BI619097   BI619869   BI623880   BI623946   BI625052   BI625547   BI627238   BI629867   BI630172   BI632905   BI634391   BI638863   BI640071   BI641924   BP540898   BP540899   BP545130   BP545131   BP545951   BP546361   BP546387   BP546467   BP546587   BP547604   BP552223   BP557969   BP557970   BP558195   BQ102631   BQ102657   BQ102804   BQ103070   BQ103711   BT029274   BT099556   BT120310   BX047828   BX058218   BX064498   CK086545   CK131576   CK604996   CK604997   CO152848   CO153231   CO153545   CO154732   CO154735   CO155579   CO156686   CO157027   CO178972   CO180093   CO185313   CO187085   CO188612   CO189241   CO190085   CO194413   CO194437   CO264105   CO266008   CO266326   CO269049   CO270252   CO271644   CO272195   CO274142   CO277204   CO280103   CO281039   CO281103   CO284015   CO285165   CO288324   CO291032   CO297409   CO298153   CO299573   CO302319   CO307714   CO309656   CO313099   CO318797   CO318869   CO319513   CO320921   CO322640   CO335683   CO335795   CO336243   CO337099   CO337808   CO338294   CZ470936   CZ470942   CZ470943   CZ478211   CZ481589   CZ487692   CZ487693   DQ443318   EC057353   EC057557   EC061643   EC063621   EC069814   EC070585   EC071623   EC073675   EC074343   EC076263   EC076553   EC076648   EC078418   EC082260   EC082552   EC085315   EC085413   EC085699   EC086000   EC087337   EC087733   EC195301   EC198125   EC198406   EC202133   EC203656   EC204472   EC206431   EC209132   EC210397   EC211002   EC213173   EC214449   EC222185   EC223334   EC224780   EC227338   EC230592   EC236284   EC237380   EC237502   EC245028   EC250153   EC253293   EC260870   EC261115   EC264171   EC264887   EC265001   EC265303   EC265862   EC266162   EC266331   EC267735   EC267881   EL881624   EL881696   EL881703   EV576800   EV577648   EV578664   EV582246   EV585255   EV589264   EV590943   EV594907   EV595519   EV597425   EV598079   EV598632   GH943886   GH951013   GH951278   GH953313   GH955706   GH955899   GH955995   GH957597   GH960492   GH965752   GH965895   GH971819   GH973108   GH974199   GH974901   GH977814   GH979689   GH979807   GH979936   GH980813   U84897 AAC47519   U84898 AAC47520
UniProtKB/Swiss-Prot	P92177
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage   presumptive embryonic/larval peripheral nervous system   (Tien et al., 1999) presumptive embryonic/larval central nervous system   (Tien et al., 1999) embryonic stage 4     (Tien et al., 1999) embryonic stage 7 -- 8   ventral midline   (Tien et al., 1999) cephalic furrow   (Tien et al., 1999) amnioproctodeal invagination   (Tien et al., 1999) anterior transverse furrow   (Tien et al., 1999) posterior transverse furrow   (Tien et al., 1999) dorsal ectoderm   (Tien et al., 1999) embryonic stage 9 -- 10   ventral nerve cord primordium   (Tien et al., 1999) adult stage   ring canal   (Benton et al., 2002) germarium   (Benton et al., 2002) mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 7 of adulthood -- day 49 of adulthood   adult heart   (Cammarato et al., 2011)
Additional Descriptive Data
	In syncytial embryos, 14-3-3ε protein localizes to rapidly dividing nuclei. During mitosis, it is retained in nuclei. During cellularization, nuclear expression diminishes and membrane expression appears. During gastrulation, a low level of staining is observed in ectodermal cells. High levels are transiently observed in various tissues. At stages 7 and 8, expression is observed in several invaginating furrows, the ventral midline cells, and the dorsal epidermal cells. At stages 9 and 10, prominant staining is observed in the ventral neurogenic region extending in a 4-5 cell wide region from the midline. 14-3-3ε protein is highly enriched in the central and peripheral nervous systems. It is observed in the cytoplasm of neurons. (Tien et al., 1999)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
chromosome inferred from direct assay (Su et al., 2001) cytoplasm inferred from direct assay (Su et al., 2001) germline ring canal inferred from direct assay (Benton et al., 2002) microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Su et al., 2001)
Expression Deduced from Reporters
Reporter: P{lacW}14-3-3εj2B10 Stage Tissue/Position (including subcellular localization) Reference wandering third instar larval stage   embryonic/larval midgut | restricted   (Meister and Braun, 1995.10) embryonic/larval salivary gland   (Meister and Braun, 1995.10) gastric caecum   (Meister and Braun, 1995.10) third instar larval stage L2   Malpighian tubule | restricted   (Meister and Braun, 1995.10)
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2667.9 Larval Midgut    2656.8 Larval Hindgut    3107.4 Larval Malpighian Tubules    3015.2 Larval Fat Body    2238.1 Larval Salivary Gland    2959.2 Larval Trachea    4322.3 Larval Carcass    2122.375 Adult Head    1673.3 Adult Eye    3688.35 Adult Brain    2227.2 Adult Thoracic-Abdominal Ganglion    1946.2 Adult Crop    1969.7 Adult Midgut    2008.9 Adult Hindgut    1991.7 Adult Malpighian Tubules    2814.1 Adult Fat Body    2214.2 Adult Salivary Gland    1584.5 Adult Heart    2741.925 Adult VirginFemale Spermatheca    2373.9 Adult InseminatedFemale Spermatheca    2164.5 Adult Ovary    2469.5 Adult Testis    1584.7 Adult Male Accessory Gland    2425.4 Adult Carcass    1299 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (2667.9) Larval Midgut  (2656.8) Larval Hindgut  (3107.4) Larval Malpighian Tubules  (3015.2) Larval Fat Body  (2238.1) Larval Salivary Gland  (2959.2) Larval Trachea  (4322.3) Larval Carcass  (2122.375) Adult Head  (1673.3) Adult Eye  (3688.35) Adult Brain  (2227.2) Adult Thoracic-Abdominal Ganglion  (1946.2) Adult Crop  (1969.7) Adult Midgut  (2008.9) Adult Hindgut  (1991.7) Adult Malpighian Tubules  (2814.1) Adult Fat Body  (2214.2) Adult Salivary Gland  (1584.5) Adult Heart  (2741.925) Adult VirginFemale Spermatheca  (2373.9) Adult InseminatedFemale Spermatheca  (2164.5) Adult Ovary  (2469.5) Adult Testis  (1584.7) Adult Male Accessory Gland  (2425.4) Adult Carcass  (1299) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (2667.9) Larval Midgut  (2656.8) Larval Hindgut  (3107.4) Larval Malpighian Tubules  (3015.2) Larval Fat Body  (2238.1) Larval Salivary Gland  (2959.2) Larval Trachea  (4322.3) Larval Carcass  (2122.375) Adult Head  (1673.3) Adult Eye  (3688.35) Adult Brain  (2227.2) Adult Thoracic-Abdominal Ganglion  (1946.2) Adult Crop  (1969.7) Adult Midgut  (2008.9) Adult Hindgut  (1991.7) Adult Malpighian Tubules  (2814.1) Adult Fat Body  (2214.2) Adult Salivary Gland  (1584.5) Adult Heart  (2741.925) Adult VirginFemale Spermatheca  (2373.9) Adult InseminatedFemale Spermatheca  (2164.5) Adult Ovary  (2469.5) Adult Testis  (1584.7) Adult Male Accessory Gland  (2425.4) Adult Carcass  (1299) Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2667.9 Larval Midgut    2656.8 Larval Hindgut    3107.4 Larval Malpighian Tubules    3015.2 Larval Fat Body    2238.1 Larval Salivary Gland    2959.2 Larval Trachea    4322.3 Larval Carcass    2122.375 Adult Head    1673.3 Adult Eye    3688.35 Adult Brain    2227.2 Adult Thoracic-Abdominal Ganglion    1946.2 Adult Crop    1969.7 Adult Midgut    2008.9 Adult Hindgut    1991.7 Adult Malpighian Tubules    2814.1 Adult Fat Body    2214.2 Adult Salivary Gland    1584.5 Adult Heart    2741.925 Adult VirginFemale Spermatheca    2373.9 Adult InseminatedFemale Spermatheca    2164.5 Adult Ovary    2469.5 Adult Testis    1584.7 Adult Male Accessory Gland    2425.4 Adult Carcass    1299 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    2667.9 Larval Midgut    2656.8 Larval Hindgut    3107.4 Larval Malpighian Tubules    3015.2 Larval Fat Body    2238.1 Larval Salivary Gland    2959.2 Larval Trachea    4322.3 Larval Carcass    2122.375 Adult Head    1673.3 Adult Eye    3688.35 Adult Brain    2227.2 Adult Thoracic-Abdominal Ganglion    1946.2 Adult Crop    1969.7 Adult Midgut    2008.9 Adult Hindgut    1991.7 Adult Malpighian Tubules    2814.1 Adult Fat Body    2214.2 Adult Salivary Gland    1584.5 Adult Heart    2741.925 Adult VirginFemale Spermatheca    2373.9 Adult InseminatedFemale Spermatheca    2164.5 Adult Ovary    2469.5 Adult Testis    1584.7 Adult Male Accessory Gland    2425.4 Adult Carcass    1299 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (2667.9) Larval Midgut  (2656.8) Larval Hindgut  (3107.4) Larval Malpighian Tubules  (3015.2) Larval Fat Body  (2238.1) Larval Salivary Gland  (2959.2) Larval Trachea  (4322.3) Larval Carcass  (2122.375) Adult Head  (1673.3) Adult Eye  (3688.35) Adult Brain  (2227.2) Adult Thoracic-Abdominal Ganglion  (1946.2) Adult Crop  (1969.7) Adult Midgut  (2008.9) Adult Hindgut  (1991.7) Adult Malpighian Tubules  (2814.1) Adult Fat Body  (2214.2) Adult Salivary Gland  (1584.5) Adult Heart  (2741.925) Adult VirginFemale Spermatheca  (2373.9) Adult InseminatedFemale Spermatheca  (2164.5) Adult Ovary  (2469.5) Adult Testis  (1584.7) Adult Male Accessory Gland  (2425.4) Adult Carcass  (1299) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (2667.9) Larval Midgut  (2656.8) Larval Hindgut  (3107.4) Larval Malpighian Tubules  (3015.2) Larval Fat Body  (2238.1) Larval Salivary Gland  (2959.2) Larval Trachea  (4322.3) Larval Carcass  (2122.375) Adult Head  1673.3 Adult Eye  (3688.35) Adult Brain  (2227.2) Adult Thoracic-Abdominal Ganglion  (1946.2) Adult Crop  (1969.7) Adult Midgut  (2008.9) Adult Hindgut  (1991.7) Adult Malpighian Tubules  (2814.1) Adult Fat Body  (2214.2) Adult Salivary Gland  1584.5 Adult Heart  (2741.925) Adult VirginFemale Spermatheca  (2373.9) Adult InseminatedFemale Spermatheca  (2164.5) Adult Ovary  (2469.5) Adult Testis  1584.7 Adult Male Accessory Gland  (2425.4) Adult Carcass  1299 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2667.9 Larval Midgut    2656.8 Larval Hindgut    3107.4 Larval Malpighian Tubules    3015.2 Larval Fat Body    2238.1 Larval Salivary Gland    2959.2 Larval Trachea    4322.3 Larval Carcass    2122.375 Adult Head    1673.3 Adult Eye    3688.35 Adult Brain    2227.2 Adult Thoracic-Abdominal Ganglion    1946.2 Adult Crop    1969.7 Adult Midgut    2008.9 Adult Hindgut    1991.7 Adult Malpighian Tubules    2814.1 Adult Fat Body    2214.2 Adult Salivary Gland    1584.5 Adult Heart    2741.925 Adult VirginFemale Spermatheca    2373.9 Adult InseminatedFemale Spermatheca    2164.5 Adult Ovary    2469.5 Adult Testis    1584.7 Adult Male Accessory Gland    2425.4 Adult Carcass    1299 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    1673.3   NA  Eye    3688.35   NA  Brain    2227.2   2667.9  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    1946.2   NA  Crop    1969.7   2656.8  Midgut    2008.9   3107.4  Hindgut    1991.7   3015.2  Malpighian Tubules    2814.1   2238.1  Fat Body    2214.2   2959.2  Salivary Gland    1584.5   NA  Heart    2741.925   4322.3  Trachea    NA   NA  VirginFemale Spermatheca    2373.9   NA  InseminatedFemale Spermatheca    2164.5   NA  Ovary    2469.5   NA  Testis    1584.7   NA  Male Accessory Gland    2425.4   2122.375  Carcass    1299 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1597 central nervous system, larvae L3    5261 central nervous system, pupae P8    4042 head, virgin 1-day female    1593 head, virgin 4-day female    1828 head, virgin 20-day female    372 head, mated 1-day female    2002 head, mated 4-day female    1198 head, mated 20-day female    552 head, mated 1-day male    1714 head, mated 4-day male    1539 head, mated 20-day male    1263 salivary gland, larvae L3 wandering    384 salivary gland, white prepupae    901 digestive system, larvae L3 wandering    1528 digestive system, 1-day adult    775 digestive system, 4-day adult    1095 digestive system, 20-day adult    790 fat body, larvae L3 wandering    790 fat body, white prepupae    792 fat body, pupae P8    1746 carcass, larvae L3 wandering    1587 carcass, 1-day adult    685 carcass, 4-day adult    985 carcass, 20-day adult    690 ovary, virgin 4-day female    1582 ovary, mated 4-day female    4682 testis, mated 4-day male    786 accessory gland, mated 4-day male    735 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1597) central nervous system, larvae L3  (5261) central nervous system, pupae P8  (4042) head, virgin 1-day female  (1593) head, virgin 4-day female  (1828) head, virgin 20-day female  (372) head, mated 1-day female  (2002) head, mated 4-day female  (1198) head, mated 20-day female  (552) head, mated 1-day male  (1714) head, mated 4-day male  (1539) head, mated 20-day male  (1263) salivary gland, larvae L3 wandering  (384) salivary gland, white prepupae  (901) digestive system, larvae L3 wandering  (1528) digestive system, 1-day adult  (775) digestive system, 4-day adult  (1095) digestive system, 20-day adult  (790) fat body, larvae L3 wandering  (790) fat body, white prepupae  (792) fat body, pupae P8  (1746) carcass, larvae L3 wandering  (1587) carcass, 1-day adult  (685) carcass, 4-day adult  (985) carcass, 20-day adult  (690) ovary, virgin 4-day female  (1582) ovary, mated 4-day female  (4682) testis, mated 4-day male  (786) accessory gland, mated 4-day male  (735) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1597) central nervous system, larvae L3  (5261) central nervous system, pupae P8  (4042) head, virgin 1-day female  (1593) head, virgin 4-day female  (1828) head, virgin 20-day female  (372) head, mated 1-day female  (2002) head, mated 4-day female  (1198) head, mated 20-day female  (552) head, mated 1-day male  (1714) head, mated 4-day male  (1539) head, mated 20-day male  (1263) salivary gland, larvae L3 wandering  (384) salivary gland, white prepupae  (901) digestive system, larvae L3 wandering  (1528) digestive system, 1-day adult  (775) digestive system, 4-day adult  (1095) digestive system, 20-day adult  (790) fat body, larvae L3 wandering  (790) fat body, white prepupae  (792) fat body, pupae P8  (1746) carcass, larvae L3 wandering  (1587) carcass, 1-day adult  (685) carcass, 4-day adult  (985) carcass, 20-day adult  (690) ovary, virgin 4-day female  (1582) ovary, mated 4-day female  (4682) testis, mated 4-day male  (786) accessory gland, mated 4-day male  (735) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1597 central nervous system, larvae L3    5261 central nervous system, pupae P8    4042 head, virgin 1-day female    1593 head, virgin 4-day female    1828 head, virgin 20-day female    372 head, mated 1-day female    2002 head, mated 4-day female    1198 head, mated 20-day female    552 head, mated 1-day male    1714 head, mated 4-day male    1539 head, mated 20-day male    1263 salivary gland, larvae L3 wandering    384 salivary gland, white prepupae    901 digestive system, larvae L3 wandering    1528 digestive system, 1-day adult    775 digestive system, 4-day adult    1095 digestive system, 20-day adult    790 fat body, larvae L3 wandering    790 fat body, white prepupae    792 fat body, pupae P8    1746 carcass, larvae L3 wandering    1587 carcass, 1-day adult    685 carcass, 4-day adult    985 carcass, 20-day adult    690 ovary, virgin 4-day female    1582 ovary, mated 4-day female    4682 testis, mated 4-day male    786 accessory gland, mated 4-day male    735 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    1597 central nervous system, larvae L3    5261 central nervous system, pupae P8    4042 head, virgin 1-day female    1593 head, virgin 4-day female    1828 head, virgin 20-day female    372 head, mated 1-day female    2002 head, mated 4-day female    1198 head, mated 20-day female    552 head, mated 1-day male    1714 head, mated 4-day male    1539 head, mated 20-day male    1263 salivary gland, larvae L3 wandering    384 salivary gland, white prepupae    901 digestive system, larvae L3 wandering    1528 digestive system, 1-day adult    775 digestive system, 4-day adult    1095 digestive system, 20-day adult    790 fat body, larvae L3 wandering    790 fat body, white prepupae    792 fat body, pupae P8    1746 carcass, larvae L3 wandering    1587 carcass, 1-day adult    685 carcass, 4-day adult    985 carcass, 20-day adult    690 ovary, virgin 4-day female    1582 ovary, mated 4-day female    4682 testis, mated 4-day male    786 accessory gland, mated 4-day male    735 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1597) central nervous system, larvae L3  (5261) central nervous system, pupae P8  (4042) head, virgin 1-day female  (1593) head, virgin 4-day female  (1828) head, virgin 20-day female  (372) head, mated 1-day female  (2002) head, mated 4-day female  (1198) head, mated 20-day female  (552) head, mated 1-day male  (1714) head, mated 4-day male  (1539) head, mated 20-day male  (1263) salivary gland, larvae L3 wandering  (384) salivary gland, white prepupae  (901) digestive system, larvae L3 wandering  (1528) digestive system, 1-day adult  (775) digestive system, 4-day adult  (1095) digestive system, 20-day adult  (790) fat body, larvae L3 wandering  (790) fat body, white prepupae  (792) fat body, pupae P8  (1746) carcass, larvae L3 wandering  (1587) carcass, 1-day adult  (685) carcass, 4-day adult  (985) carcass, 20-day adult  (690) ovary, virgin 4-day female  (1582) ovary, mated 4-day female  (4682) testis, mated 4-day male  (786) accessory gland, mated 4-day male  (735) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (1597) central nervous system, larvae L3  (5261) central nervous system, pupae P8  (4042) head, virgin 1-day female  (1593) head, virgin 4-day female  (1828) head, virgin 20-day female  (372) head, mated 1-day female  (2002) head, mated 4-day female  (1198) head, mated 20-day female  (552) head, mated 1-day male  (1714) head, mated 4-day male  (1539) head, mated 20-day male  (1263) salivary gland, larvae L3 wandering  (384) salivary gland, white prepupae  (901) digestive system, larvae L3 wandering  (1528) digestive system, 1-day adult  (775) digestive system, 4-day adult  (1095) digestive system, 20-day adult  (790) fat body, larvae L3 wandering  (790) fat body, white prepupae  (792) fat body, pupae P8  (1746) carcass, larvae L3 wandering  (1587) carcass, 1-day adult  (685) carcass, 4-day adult  (985) carcass, 20-day adult  (690) ovary, virgin 4-day female  (1582) ovary, mated 4-day female  (4682) testis, mated 4-day male  (786) accessory gland, mated 4-day male  (735) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    1597 central nervous system, larvae L3    5261 central nervous system, pupae P8    4042 head, virgin 1-day female    1593 head, virgin 4-day female    1828 head, virgin 20-day female    372 head, mated 1-day female    2002 head, mated 4-day female    1198 head, mated 20-day female    552 head, mated 1-day male    1714 head, mated 4-day male    1539 head, mated 20-day male    1263 salivary gland, larvae L3 wandering    384 salivary gland, white prepupae    901 digestive system, larvae L3 wandering    1528 digestive system, 1-day adult    775 digestive system, 4-day adult    1095 digestive system, 20-day adult    790 fat body, larvae L3 wandering    790 fat body, white prepupae    792 fat body, pupae P8    1746 carcass, larvae L3 wandering    1587 carcass, 1-day adult    685 carcass, 4-day adult    985 carcass, 20-day adult    690 ovary, virgin 4-day female    1582 ovary, mated 4-day female    4682 testis, mated 4-day male    786 accessory gland, mated 4-day male    735 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Very high expression at all stages of development. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    912 embryo 04-06hr    965 embryo 06-08hr    644 embryo 08-10hr    566 embryo 10-12hr    488 embryo 12-14hr    437 embryo 14-16hr    364 embryo 16-18hr    381 embryo 18-20hr    266 embryo 20-22hr    302 embryo 22-24hr    325 larva L1    277 larva L2    301 larva L3 12hr old    245 larva L3 puffstage 1-2    258 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    408 white prepupae new    441 white prepupae 12hr    497 white prepupae 24hr    500 pupae 2d postWPP    308 pupae 3d postWPP    145 pupae 4d postWPP    134 adult male 01day    152 adult male 05day    163 adult male 30day    156 adult female 01day    246 adult female 05day    364 adult female 30day    337 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (482) embryo 02-04hr  (912) embryo 04-06hr  (965) embryo 06-08hr  (644) embryo 08-10hr  (566) embryo 10-12hr  (488) embryo 12-14hr  (437) embryo 14-16hr  (364) embryo 16-18hr  (381) embryo 18-20hr  (266) embryo 20-22hr  (302) embryo 22-24hr  (325) larva L1  (277) larva L2  (301) larva L3 12hr old  (245) larva L3 puffstage 1-2  (258) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (408) white prepupae new  (441) white prepupae 12hr  (497) white prepupae 24hr  (500) pupae 2d postWPP  (308) pupae 3d postWPP  (145) pupae 4d postWPP  (134) adult male 01day  (152) adult male 05day  (163) adult male 30day  (156) adult female 01day  (246) adult female 05day  (364) adult female 30day  (337) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (482) embryo 02-04hr  (912) embryo 04-06hr  (965) embryo 06-08hr  (644) embryo 08-10hr  (566) embryo 10-12hr  (488) embryo 12-14hr  (437) embryo 14-16hr  (364) embryo 16-18hr  (381) embryo 18-20hr  (266) embryo 20-22hr  (302) embryo 22-24hr  (325) larva L1  (277) larva L2  (301) larva L3 12hr old  (245) larva L3 puffstage 1-2  (258) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (408) white prepupae new  (441) white prepupae 12hr  (497) white prepupae 24hr  (500) pupae 2d postWPP  (308) pupae 3d postWPP  (145) pupae 4d postWPP  (134) adult male 01day  (152) adult male 05day  (163) adult male 30day  (156) adult female 01day  (246) adult female 05day  (364) adult female 30day  (337) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    912 embryo 04-06hr    965 embryo 06-08hr    644 embryo 08-10hr    566 embryo 10-12hr    488 embryo 12-14hr    437 embryo 14-16hr    364 embryo 16-18hr    381 embryo 18-20hr    266 embryo 20-22hr    302 embryo 22-24hr    325 larva L1    277 larva L2    301 larva L3 12hr old    245 larva L3 puffstage 1-2    258 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    408 white prepupae new    441 white prepupae 12hr    497 white prepupae 24hr    500 pupae 2d postWPP    308 pupae 3d postWPP    145 pupae 4d postWPP    134 adult male 01day    152 adult male 05day    163 adult male 30day    156 adult female 01day    246 adult female 05day    364 adult female 30day    337 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    912 embryo 04-06hr    965 embryo 06-08hr    644 embryo 08-10hr    566 embryo 10-12hr    488 embryo 12-14hr    437 embryo 14-16hr    364 embryo 16-18hr    381 embryo 18-20hr    266 embryo 20-22hr    302 embryo 22-24hr    325 larva L1    277 larva L2    301 larva L3 12hr old    245 larva L3 puffstage 1-2    258 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    408 white prepupae new    441 white prepupae 12hr    497 white prepupae 24hr    500 pupae 2d postWPP    308 pupae 3d postWPP    145 pupae 4d postWPP    134 adult male 01day    152 adult male 05day    163 adult male 30day    156 adult female 01day    246 adult female 05day    364 adult female 30day    337 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (482) embryo 02-04hr  (912) embryo 04-06hr  (965) embryo 06-08hr  (644) embryo 08-10hr  (566) embryo 10-12hr  (488) embryo 12-14hr  (437) embryo 14-16hr  (364) embryo 16-18hr  (381) embryo 18-20hr  (266) embryo 20-22hr  (302) embryo 22-24hr  (325) larva L1  (277) larva L2  (301) larva L3 12hr old  (245) larva L3 puffstage 1-2  (258) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (408) white prepupae new  (441) white prepupae 12hr  (497) white prepupae 24hr  (500) pupae 2d postWPP  (308) pupae 3d postWPP  (145) pupae 4d postWPP  (134) adult male 01day  (152) adult male 05day  (163) adult male 30day  (156) adult female 01day  (246) adult female 05day  (364) adult female 30day  (337) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (482) embryo 02-04hr  (912) embryo 04-06hr  (965) embryo 06-08hr  (644) embryo 08-10hr  (566) embryo 10-12hr  (488) embryo 12-14hr  (437) embryo 14-16hr  (364) embryo 16-18hr  (381) embryo 18-20hr  (266) embryo 20-22hr  (302) embryo 22-24hr  (325) larva L1  (277) larva L2  (301) larva L3 12hr old  (245) larva L3 puffstage 1-2  (258) larva L3 puffstage 3-6  (336) larva L3 puffstage 7-9  (408) white prepupae new  (441) white prepupae 12hr  (497) white prepupae 24hr  (500) pupae 2d postWPP  (308) pupae 3d postWPP  (145) pupae 4d postWPP  134 adult male 01day  (152) adult male 05day  (163) adult male 30day  (156) adult female 01day  (246) adult female 05day  (364) adult female 30day  (337) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    482 embryo 02-04hr    912 embryo 04-06hr    965 embryo 06-08hr    644 embryo 08-10hr    566 embryo 10-12hr    488 embryo 12-14hr    437 embryo 14-16hr    364 embryo 16-18hr    381 embryo 18-20hr    266 embryo 20-22hr    302 embryo 22-24hr    325 larva L1    277 larva L2    301 larva L3 12hr old    245 larva L3 puffstage 1-2    258 larva L3 puffstage 3-6    336 larva L3 puffstage 7-9    408 white prepupae new    441 white prepupae 12hr    497 white prepupae 24hr    500 pupae 2d postWPP    308 pupae 3d postWPP    145 pupae 4d postWPP    134 adult male 01day    152 adult male 05day    163 adult male 30day    156 adult female 01day    246 adult female 05day    364 adult female 30day    337 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3268 Schneider line 2 Sg4    1681 embryonic 1182-4H    2645 embryonic GM2    2265 embryonic Kc167    2006 embryonic S1    1697 embryonic S3    2099 leg disc CME L1    2387 wing disc CME-W2    1840 wing disc ML-DmD8    1722 wing disc ML-DmD9    1868 wing disc ML-DmD16-c3    2512 wing disc ML-DmD21    2134 wing disc ML-DmD32    3087 haltere disc ML-DmD17-c3    2281 eye-antennal disc ML-DmD11    1263 antennal disc ML-DmD20-c5    1975 mixed discs ML-DmD4-c1    3523 CNS ML-DmBG1-c1    2580 CNS ML-DmBG2-c2    1557 tumorous blood cells mbn2    1760 ovary fGS/OSS    6822 ovary OSC    9041 ovary OSS    6526 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (3268) Schneider line 2 Sg4  (1681) embryonic 1182-4H  (2645) embryonic GM2  (2265) embryonic Kc167  (2006) embryonic S1  (1697) embryonic S3  (2099) leg disc CME L1  (2387) wing disc CME-W2  (1840) wing disc ML-DmD8  (1722) wing disc ML-DmD9  (1868) wing disc ML-DmD16-c3  (2512) wing disc ML-DmD21  (2134) wing disc ML-DmD32  (3087) haltere disc ML-DmD17-c3  (2281) eye-antennal disc ML-DmD11  (1263) antennal disc ML-DmD20-c5  (1975) mixed discs ML-DmD4-c1  (3523) CNS ML-DmBG1-c1  (2580) CNS ML-DmBG2-c2  (1557) tumorous blood cells mbn2  (1760) ovary fGS/OSS  (6822) ovary OSC  (9041) ovary OSS  (6526) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (3268) Schneider line 2 Sg4  (1681) embryonic 1182-4H  (2645) embryonic GM2  (2265) embryonic Kc167  (2006) embryonic S1  (1697) embryonic S3  (2099) leg disc CME L1  (2387) wing disc CME-W2  (1840) wing disc ML-DmD8  (1722) wing disc ML-DmD9  (1868) wing disc ML-DmD16-c3  (2512) wing disc ML-DmD21  (2134) wing disc ML-DmD32  (3087) haltere disc ML-DmD17-c3  (2281) eye-antennal disc ML-DmD11  (1263) antennal disc ML-DmD20-c5  (1975) mixed discs ML-DmD4-c1  (3523) CNS ML-DmBG1-c1  (2580) CNS ML-DmBG2-c2  (1557) tumorous blood cells mbn2  (1760) ovary fGS/OSS  (6822) ovary OSC  (9041) ovary OSS  (6526) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3268 Schneider line 2 Sg4    1681 embryonic 1182-4H    2645 embryonic GM2    2265 embryonic Kc167    2006 embryonic S1    1697 embryonic S3    2099 leg disc CME L1    2387 wing disc CME-W2    1840 wing disc ML-DmD8    1722 wing disc ML-DmD9    1868 wing disc ML-DmD16-c3    2512 wing disc ML-DmD21    2134 wing disc ML-DmD32    3087 haltere disc ML-DmD17-c3    2281 eye-antennal disc ML-DmD11    1263 antennal disc ML-DmD20-c5    1975 mixed discs ML-DmD4-c1    3523 CNS ML-DmBG1-c1    2580 CNS ML-DmBG2-c2    1557 tumorous blood cells mbn2    1760 ovary fGS/OSS    6822 ovary OSC    9041 ovary OSS    6526 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    3268 Schneider line 2 Sg4    1681 embryonic 1182-4H    2645 embryonic GM2    2265 embryonic Kc167    2006 embryonic S1    1697 embryonic S3    2099 leg disc CME L1    2387 wing disc CME-W2    1840 wing disc ML-DmD8    1722 wing disc ML-DmD9    1868 wing disc ML-DmD16-c3    2512 wing disc ML-DmD21    2134 wing disc ML-DmD32    3087 haltere disc ML-DmD17-c3    2281 eye-antennal disc ML-DmD11    1263 antennal disc ML-DmD20-c5    1975 mixed discs ML-DmD4-c1    3523 CNS ML-DmBG1-c1    2580 CNS ML-DmBG2-c2    1557 tumorous blood cells mbn2    1760 ovary fGS/OSS    6822 ovary OSC    9041 ovary OSS    6526 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (3268) Schneider line 2 Sg4  (1681) embryonic 1182-4H  (2645) embryonic GM2  (2265) embryonic Kc167  (2006) embryonic S1  (1697) embryonic S3  (2099) leg disc CME L1  (2387) wing disc CME-W2  (1840) wing disc ML-DmD8  (1722) wing disc ML-DmD9  (1868) wing disc ML-DmD16-c3  (2512) wing disc ML-DmD21  (2134) wing disc ML-DmD32  (3087) haltere disc ML-DmD17-c3  (2281) eye-antennal disc ML-DmD11  (1263) antennal disc ML-DmD20-c5  (1975) mixed discs ML-DmD4-c1  (3523) CNS ML-DmBG1-c1  (2580) CNS ML-DmBG2-c2  (1557) tumorous blood cells mbn2  (1760) ovary fGS/OSS  (6822) ovary OSC  (9041) ovary OSS  (6526) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (3268) Schneider line 2 Sg4  (1681) embryonic 1182-4H  (2645) embryonic GM2  (2265) embryonic Kc167  (2006) embryonic S1  (1697) embryonic S3  (2099) leg disc CME L1  (2387) wing disc CME-W2  (1840) wing disc ML-DmD8  (1722) wing disc ML-DmD9  (1868) wing disc ML-DmD16-c3  (2512) wing disc ML-DmD21  (2134) wing disc ML-DmD32  (3087) haltere disc ML-DmD17-c3  (2281) eye-antennal disc ML-DmD11  (1263) antennal disc ML-DmD20-c5  (1975) mixed discs ML-DmD4-c1  (3523) CNS ML-DmBG1-c1  (2580) CNS ML-DmBG2-c2  (1557) tumorous blood cells mbn2  (1760) ovary fGS/OSS  (6822) ovary OSC  (9041) ovary OSS  (6526) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    3268 Schneider line 2 Sg4    1681 embryonic 1182-4H    2645 embryonic GM2    2265 embryonic Kc167    2006 embryonic S1    1697 embryonic S3    2099 leg disc CME L1    2387 wing disc CME-W2    1840 wing disc ML-DmD8    1722 wing disc ML-DmD9    1868 wing disc ML-DmD16-c3    2512 wing disc ML-DmD21    2134 wing disc ML-DmD32    3087 haltere disc ML-DmD17-c3    2281 eye-antennal disc ML-DmD11    1263 antennal disc ML-DmD20-c5    1975 mixed discs ML-DmD4-c1    3523 CNS ML-DmBG1-c1    2580 CNS ML-DmBG2-c2    1557 tumorous blood cells mbn2    1760 ovary fGS/OSS    6822 ovary OSC    9041 ovary OSS    6526 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4729 cold shock, 4-day adult    4892 heat shock, 4-day adult    1156 Cadmium 50 mM 6 hrs, larvae L3    831 Cadmium 50 mM 12 hrs, larvae L3    1218 Cadmium 50 mM 48 hrs, 4-day adult    1644 Cadmium 100 mM 48 hrs, 4-day adult    3315 Copper 0.5 mM 12 hrs, larvae L3    2396 Copper 15 mM 48 hrs, 4-day adult    5482 Zinc 5 mM 12 hrs, larvae L3    3789 Zinc 4.5 mM 48 hrs, 4-day adult    4257 Ethanol 2.5% 3 hrs, larvae L3    1083 Ethanol 5% 3 hrs, larvae L3    990 Ethanol 10% 3 hrs, larvae L3    1050 Caffeine 1.5 mg/ml 4 hrs, larvae L3    651 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3209 Caffeine 25 mg/ml 48 hrs, 4-day adult    6904 Paraquat 5 mM 48 hrs, 4-day adult    2219 Paraquat 10 mM 48 hrs, 4-day adult    1020 Rotenone 2 μg 12 hrs, larvae L3    889 Rotenone 8 μg 12 hrs, larvae L3    497 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (4729) cold shock, 4-day adult  (4892) heat shock, 4-day adult  (1156) Cadmium 50 mM 6 hrs, larvae L3  (831) Cadmium 50 mM 12 hrs, larvae L3  (1218) Cadmium 50 mM 48 hrs, 4-day adult  (1644) Cadmium 100 mM 48 hrs, 4-day adult  (3315) Copper 0.5 mM 12 hrs, larvae L3  (2396) Copper 15 mM 48 hrs, 4-day adult  (5482) Zinc 5 mM 12 hrs, larvae L3  (3789) Zinc 4.5 mM 48 hrs, 4-day adult  (4257) Ethanol 2.5% 3 hrs, larvae L3  (1083) Ethanol 5% 3 hrs, larvae L3  (990) Ethanol 10% 3 hrs, larvae L3  (1050) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (651) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (3209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (6904) Paraquat 5 mM 48 hrs, 4-day adult  (2219) Paraquat 10 mM 48 hrs, 4-day adult  (1020) Rotenone 2 μg 12 hrs, larvae L3  (889) Rotenone 8 μg 12 hrs, larvae L3  (497) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (4729) cold shock, 4-day adult  (4892) heat shock, 4-day adult  (1156) Cadmium 50 mM 6 hrs, larvae L3  (831) Cadmium 50 mM 12 hrs, larvae L3  (1218) Cadmium 50 mM 48 hrs, 4-day adult  (1644) Cadmium 100 mM 48 hrs, 4-day adult  (3315) Copper 0.5 mM 12 hrs, larvae L3  (2396) Copper 15 mM 48 hrs, 4-day adult  (5482) Zinc 5 mM 12 hrs, larvae L3  (3789) Zinc 4.5 mM 48 hrs, 4-day adult  (4257) Ethanol 2.5% 3 hrs, larvae L3  (1083) Ethanol 5% 3 hrs, larvae L3  (990) Ethanol 10% 3 hrs, larvae L3  (1050) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (651) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (3209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (6904) Paraquat 5 mM 48 hrs, 4-day adult  (2219) Paraquat 10 mM 48 hrs, 4-day adult  (1020) Rotenone 2 μg 12 hrs, larvae L3  (889) Rotenone 8 μg 12 hrs, larvae L3  (497) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4729 cold shock, 4-day adult    4892 heat shock, 4-day adult    1156 Cadmium 50 mM 6 hrs, larvae L3    831 Cadmium 50 mM 12 hrs, larvae L3    1218 Cadmium 50 mM 48 hrs, 4-day adult    1644 Cadmium 100 mM 48 hrs, 4-day adult    3315 Copper 0.5 mM 12 hrs, larvae L3    2396 Copper 15 mM 48 hrs, 4-day adult    5482 Zinc 5 mM 12 hrs, larvae L3    3789 Zinc 4.5 mM 48 hrs, 4-day adult    4257 Ethanol 2.5% 3 hrs, larvae L3    1083 Ethanol 5% 3 hrs, larvae L3    990 Ethanol 10% 3 hrs, larvae L3    1050 Caffeine 1.5 mg/ml 4 hrs, larvae L3    651 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3209 Caffeine 25 mg/ml 48 hrs, 4-day adult    6904 Paraquat 5 mM 48 hrs, 4-day adult    2219 Paraquat 10 mM 48 hrs, 4-day adult    1020 Rotenone 2 μg 12 hrs, larvae L3    889 Rotenone 8 μg 12 hrs, larvae L3    497 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4729 cold shock, 4-day adult    4892 heat shock, 4-day adult    1156 Cadmium 50 mM 6 hrs, larvae L3    831 Cadmium 50 mM 12 hrs, larvae L3    1218 Cadmium 50 mM 48 hrs, 4-day adult    1644 Cadmium 100 mM 48 hrs, 4-day adult    3315 Copper 0.5 mM 12 hrs, larvae L3    2396 Copper 15 mM 48 hrs, 4-day adult    5482 Zinc 5 mM 12 hrs, larvae L3    3789 Zinc 4.5 mM 48 hrs, 4-day adult    4257 Ethanol 2.5% 3 hrs, larvae L3    1083 Ethanol 5% 3 hrs, larvae L3    990 Ethanol 10% 3 hrs, larvae L3    1050 Caffeine 1.5 mg/ml 4 hrs, larvae L3    651 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3209 Caffeine 25 mg/ml 48 hrs, 4-day adult    6904 Paraquat 5 mM 48 hrs, 4-day adult    2219 Paraquat 10 mM 48 hrs, 4-day adult    1020 Rotenone 2 μg 12 hrs, larvae L3    889 Rotenone 8 μg 12 hrs, larvae L3    497 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult  (4729) cold shock, 4-day adult  (4892) heat shock, 4-day adult  (1156) Cadmium 50 mM 6 hrs, larvae L3  (831) Cadmium 50 mM 12 hrs, larvae L3  (1218) Cadmium 50 mM 48 hrs, 4-day adult  (1644) Cadmium 100 mM 48 hrs, 4-day adult  (3315) Copper 0.5 mM 12 hrs, larvae L3  (2396) Copper 15 mM 48 hrs, 4-day adult  (5482) Zinc 5 mM 12 hrs, larvae L3  (3789) Zinc 4.5 mM 48 hrs, 4-day adult  (4257) Ethanol 2.5% 3 hrs, larvae L3  (1083) Ethanol 5% 3 hrs, larvae L3  (990) Ethanol 10% 3 hrs, larvae L3  (1050) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (651) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (3209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (6904) Paraquat 5 mM 48 hrs, 4-day adult  (2219) Paraquat 10 mM 48 hrs, 4-day adult  (1020) Rotenone 2 μg 12 hrs, larvae L3  (889) Rotenone 8 μg 12 hrs, larvae L3  (497) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult  (4729) cold shock, 4-day adult  (4892) heat shock, 4-day adult  (1156) Cadmium 50 mM 6 hrs, larvae L3  (831) Cadmium 50 mM 12 hrs, larvae L3  (1218) Cadmium 50 mM 48 hrs, 4-day adult  (1644) Cadmium 100 mM 48 hrs, 4-day adult  (3315) Copper 0.5 mM 12 hrs, larvae L3  (2396) Copper 15 mM 48 hrs, 4-day adult  (5482) Zinc 5 mM 12 hrs, larvae L3  (3789) Zinc 4.5 mM 48 hrs, 4-day adult  (4257) Ethanol 2.5% 3 hrs, larvae L3  (1083) Ethanol 5% 3 hrs, larvae L3  (990) Ethanol 10% 3 hrs, larvae L3  (1050) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (651) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (3209) Caffeine 25 mg/ml 48 hrs, 4-day adult  (6904) Paraquat 5 mM 48 hrs, 4-day adult  (2219) Paraquat 10 mM 48 hrs, 4-day adult  (1020) Rotenone 2 μg 12 hrs, larvae L3  (889) Rotenone 8 μg 12 hrs, larvae L3  (497) Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4729 cold shock, 4-day adult    4892 heat shock, 4-day adult    1156 Cadmium 50 mM 6 hrs, larvae L3    831 Cadmium 50 mM 12 hrs, larvae L3    1218 Cadmium 50 mM 48 hrs, 4-day adult    1644 Cadmium 100 mM 48 hrs, 4-day adult    3315 Copper 0.5 mM 12 hrs, larvae L3    2396 Copper 15 mM 48 hrs, 4-day adult    5482 Zinc 5 mM 12 hrs, larvae L3    3789 Zinc 4.5 mM 48 hrs, 4-day adult    4257 Ethanol 2.5% 3 hrs, larvae L3    1083 Ethanol 5% 3 hrs, larvae L3    990 Ethanol 10% 3 hrs, larvae L3    1050 Caffeine 1.5 mg/ml 4 hrs, larvae L3    651 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3209 Caffeine 25 mg/ml 48 hrs, 4-day adult    6904 Paraquat 5 mM 48 hrs, 4-day adult    2219 Paraquat 10 mM 48 hrs, 4-day adult    1020 Rotenone 2 μg 12 hrs, larvae L3    889 Rotenone 8 μg 12 hrs, larvae L3    497 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_05 mE1_20_mRNA_expression_cluster_01
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020238 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31196-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal 14-3-3εCA06506 lethal | embryonic stage | germline clone | maternal effect 14-3-3εPL00784 lethal | embryonic stage | maternal effect 14-3-3εj2B10 lethal | larval stage | recessive 14-3-3εj2B10 lethal | partially (with Df(3R)14-3-3εex24) 14-3-3εex4 14-3-3εj2B10 lethal | partially | recessive 14-3-3εS-696 lethal | recessive 14-3-3εj2B10 14-3-3εunspecified partially lethal - majority die (with 14-3-3εex4) 14-3-3εj2B10 partially lethal - majority die (with 14-3-3εj2B10) 14-3-3εex4 partially lethal - majority die | embryonic stage | recessive 14-3-3εex4 14-3-3εj2B10 viable 14-3-3ε18A2 14-3-3εex5 14-3-3εj2B10 14-3-3εS-1259 viable, with Scer\GAL4pnr-MD237 14-3-3εGD4108 viable (with 14-3-3εex4) 14-3-3εex5 viable (with 14-3-3εex5) 14-3-3εex4 14-3-3εj2B10 viable (with 14-3-3εj2B10) 14-3-3εex5 viable (with Df(3R)14-3-3εex24) 14-3-3εex5 viable (with Df(3R)P14) 14-3-3εj2B10 viable | poor 14-3-3εS-696 14-3-3εS-1259 Sterility Allele female fertile 14-3-3ε18A2 14-3-3εex5 female fertile (with 14-3-3εex4) 14-3-3εex5 female fertile (with 14-3-3εex5) 14-3-3εex4 14-3-3εj2B10 female fertile (with 14-3-3εj2B10) 14-3-3εex5 female sterile 14-3-3εex4 14-3-3εj2B10 female sterile (with 14-3-3εex4) 14-3-3εj2B10 female sterile (with 14-3-3εj2B10) 14-3-3εex4 female sterile (with Df(3R)14-3-3εex24) 14-3-3εex4 14-3-3εj2B10 female sterile (with Df(3R)P14) 14-3-3εj2B10 female sterile | recessive 14-3-3εj2B10 fertile 14-3-3ε18A2 14-3-3εS-1259 male fertile 14-3-3εex5 male fertile (with 14-3-3εex4) 14-3-3εex5 male fertile (with 14-3-3εex5) 14-3-3εex4 14-3-3εj2B10 male fertile (with 14-3-3εj2B10) 14-3-3εex5 male sterile 14-3-3εex4 14-3-3εj2B10 male sterile (with 14-3-3εex4) 14-3-3εj2B10 male sterile (with 14-3-3εj2B10) 14-3-3εex4 male sterile (with Df(3R)14-3-3εex24) 14-3-3εex4 14-3-3εj2B10 sterile | recessive 14-3-3εj2B10 Other Phenotypes Allele body size defective (with 14-3-3εex4) 14-3-3εj2B10 body size defective (with 14-3-3εj2B10) 14-3-3εex4 body size defective | recessive 14-3-3εj2B10 long lived | dominant 14-3-3εj2B10 mitotic cell cycle defective | recessive 14-3-3εj2B10 neuroanatomy defective 14-3-3ε18A2 14-3-3εj2B10 neuroanatomy defective, with Scer\GAL4elav.PU 14-3-3εScer\UAS.T:Zzzz\FLAG neuroanatomy defective (with 14-3-3εj2B10) 14-3-3εNP1301 neuroanatomy defective (with 14-3-3εNP1301) 14-3-3εj2B10 radiation sensitive | dominant 14-3-3εj2B10 visible (with 14-3-3εex4) 14-3-3εj2B10 visible (with 14-3-3εj2B10) 14-3-3εex4 14-3-3εS-696 visible (with 14-3-3εS-696) 14-3-3εj2B10 visible | recessive 14-3-3εex4 14-3-3εj2B10 Phenotype manifest in Allele anterior crossvein 14-3-3εex4 14-3-3εj2B10 anterior crossvein (with 14-3-3εex4) 14-3-3εj2B10 anterior crossvein (with 14-3-3εj2B10) 14-3-3εex4 cycle 14 embryo 14-3-3εj2B10 embryo | germline clone | maternal effect 14-3-3εPL00784 embryonic/first instar larval cuticle | maternal effect 14-3-3εj2B10 eye (with 14-3-3εj2B10) 14-3-3εS-696 eye (with 14-3-3εS-696) 14-3-3εj2B10 eye photoreceptor cell 14-3-3εS-696 eye photoreceptor cell (with 14-3-3εj2B10) 14-3-3εS-696 eye photoreceptor cell (with 14-3-3εS-696) 14-3-3εj2B10 intersegmental nerve branch ISNb of A1-7 14-3-3ε18A2 14-3-3εj2B10 intersegmental nerve branch ISNb of A1-7, with Scer\GAL4elav.PU 14-3-3εScer\UAS.T:Zzzz\FLAG intersegmental nerve branch ISNb of A1-7 (with 14-3-3εj2B10) 14-3-3εNP1301 intersegmental nerve branch ISNb of A1-7 (with 14-3-3εNP1301) 14-3-3εj2B10 microtubule & oocyte 14-3-3εj2B10 microtubule organizing center & oocyte 14-3-3εj2B10 nurse cell | ectopic 14-3-3εj2B10 nurse cell | ectopic (with Df(3R)P14) 14-3-3εj2B10 nurse cell | ectopic | germline clone 14-3-3εj2B10 ommatidium 14-3-3εS-696 ommatidium (with 14-3-3εj2B10) 14-3-3εS-696 ommatidium (with 14-3-3εS-696) 14-3-3εj2B10 ommatidium (with Df(3R)P14) 14-3-3εS-696 oocyte 14-3-3εS-696 14-3-3εj2B10 oocyte (with Df(3R)P14) 14-3-3εj2B10 oocyte & centrosome 14-3-3εj2B10 oocyte | germline clone 14-3-3εj2B10 pole cell 14-3-3εex4 14-3-3εj2B10 pole cell, with Scer\GAL4nos.UTR.T:Hsim\VP16 14-3-3εdsRNA.Scer\UAS.cTa pole cell, with Scer\GAL4αTub84B.PL 14-3-3εdsRNA.Scer\UAS.cTa posterior crossvein 14-3-3εS-696 14-3-3εex4 14-3-3εj2B10 posterior crossvein (with 14-3-3εex4) 14-3-3εj2B10 posterior crossvein (with 14-3-3εj2B10) 14-3-3εex4 segmental nerve branch SNa of A1-7 14-3-3ε18A2 14-3-3εj2B10 segmental nerve branch SNa of A1-7 (with 14-3-3εj2B10) 14-3-3εNP1301 segmental nerve branch SNa of A1-7 (with 14-3-3εNP1301) 14-3-3εj2B10
Classical Alleles ( 26 )
For All Classical Alleles Show
Allele of 14-3-3ε Class Mutagen Stocks Known lesion 14-3-3εj2B10 loss of function allele P-element activity 2 Yes 14-3-3ε5-HA-1828 1 -- 14-3-3εc02239b 1 -- 14-3-3εc06705 piggyBac transposase 1 -- 14-3-3εe00326 piggyBac transposase 1 -- 14-3-3εe03838 piggyBac transposase 1 -- 14-3-3εEP3423 P-element activity 1 -- 14-3-3εEP3578 P-element activity 1 -- 14-3-3εf05080 piggyBac transposase 1 -- 14-3-3εNP1301 1 -- 14-3-3εS-696 antimorphic allele - genetic evidence ethyl methanesulfonate 1 Yes 14-3-3εUM-8256-3 1 -- 14-3-3ε18A2 antimorphic allele - genetic evidence ethyl methanesulfonate 0 Yes 14-3-3εc06714 piggyBac transposase 0 -- 14-3-3εCA06506 P-element activity 0 Yes 14-3-3εCPTI100026 piggyBac transposase 0 Yes 14-3-3εCPTI100034 piggyBac transposase 0 Yes 14-3-3εCPTI100045 piggyBac transposase 0 Yes 14-3-3εCPTI100065 piggyBac transposase 0 Yes 14-3-3εEP3-732 0 -- 14-3-3εex4 P-element activity 0 Yes 14-3-3εex5 P-element activity 0 Yes 14-3-3εPL00784 piggyBac transposase 0 Yes 14-3-3εS-1259 ethyl methanesulfonate 0 Yes 14-3-3εunspecified 0 -- 14-3-3εΔ24 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of 14-3-3ε Class Mutagen Stocks Known lesion 14-3-3εGD4108 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes 14-3-3εGL00366 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes 14-3-3εHM04001 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes 14-3-3εHMS01229 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes 14-3-3εKK102462 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes 14-3-3εdsRNA.Scer\UAS.cTa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes 14-3-3εdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes 14-3-3εhs.PA in vitro construct - regulatory fusion 0 Yes 14-3-3εNIG.31196R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes 14-3-3εScer\UAS.cCa in vitro construct - regulatory fusion 0 Yes 14-3-3εScer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes 14-3-3εScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes 14-3-3εScer\UAS.T:Zzzz\FLAG in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)14-3-3εex24 (Acevedo et al., 2007) Df(3R)Exel6178 (Ryder, 2004.4.26) Df(3R)P14 (Tien et al., 1999, Spradling et al., 1999, Benton et al., 2002, BDGP Project Members, 1994-1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UAS-14-3-3ε.C} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}14-3-3εEP3-732 NA P{EP}14-3-3εEP3423 NA P{EP}14-3-3εEP3578 NA miscellaneous insertions P{PTT-GA}14-3-3εCA06506 NA P{RS3}14-3-3εUM-8256-3 NA P{RS5}14-3-3ε5-HA-1828 NA PBac{544.SVS-1}14-3-3εCPTI100034 NA PBac{544.SVS-1}14-3-3εCPTI100045 NA PBac{544.SVS-1}14-3-3εCPTI100065 NA PBac{566.P.SVS-1}14-3-3εCPTI100026 NA PBac{PB}14-3-3εc02239b NA PBac{PB}14-3-3εc06705 NA PBac{PB}14-3-3εc06714 NA PBac{RB}14-3-3εe00326 NA PBac{RB}14-3-3εe03838 NA PBac{WH}14-3-3εf05080 NA insertion of enhancer trap P{lacW}14-3-3εj2B10 No P{lacW}l(3)SH081SH081 No insertion of enhancer trap binary system P{GawB}14-3-3εNP1301 No PBac{GAL4D,EYFP}14-3-3εPL00784 No
Gene Ontology: Function, Process & Cellular Component ( 26 unique terms )
Terms Based on Experimental Evidence ( 19 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with UniProtKB:Q45VV3 (assigned by UniProtKB) (Oh and Irvine, 2008) protein heterodimerization activity inferred from physical interaction with 14-3-3ζ (Messaritou et al., 2010)
Biological Process
CV term Evidence References
axon guidance inferred from mutant phenotype (Yang and Terman, 2012) determination of adult lifespan inferred from mutant phenotype (Nielsen et al., 2008) DNA damage checkpoint inferred from mutant phenotype (Su et al., 2001, Brodsky et al., 2000) germarium-derived oocyte fate determination inferred from mutant phenotype (Benton et al., 2002) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) oocyte microtubule cytoskeleton polarization inferred from mutant phenotype (Benton et al., 2002) pole cell migration inferred from mutant phenotype (Tsigkari et al., 2012) positive regulation of growth inferred from mutant phenotype (Nielsen et al., 2008) regulation of growth inferred from genetic interaction with foxo (Nielsen et al., 2008) regulation of mitosis inferred from mutant phenotype (Su et al., 2001) response to UV inferred from mutant phenotype (Nielsen et al., 2008) wing disc dorsal/ventral pattern formation inferred from genetic interaction with Bx, ap (Bejarano et al., 2008)
Cellular Component
CV term Evidence References
chromosome inferred from direct assay (Su et al., 2001) cytoplasm inferred from direct assay (Su et al., 2001) germline ring canal inferred from direct assay (Benton et al., 2002) microtubule associated complex inferred from direct assay (Hughes et al., 2008) nucleus inferred from direct assay (Su et al., 2001)
Terms Based on Predictions or Assertions ( 8 terms )
Molecular Function
CV term Evidence References
protein domain specific binding inferred from electronic annotation with InterPro:IPR000308 (FlyBase Curators et al., 2004-) protein kinase C inhibitor activity non-traceable author statement (Chang, 1997.1.10)
Biological Process
CV term Evidence References
DNA damage checkpoint traceable author statement (Sekelsky et al., 2000) imaginal disc development traceable author statement (Walworth, 2000) mitotic cell cycle checkpoint traceable author statement (Walworth, 2000) nonassociative learning non-traceable author statement (Skoulakis and Acevedo, 2003) Ras protein signal transduction non-traceable author statement (Chang, 1997.1.10, Swiss-Prot Project Members, 1998.2.1) response to radiation traceable author statement (Walworth, 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
14-3-3ε - AnxB10 experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - CG10638 experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - CG33156 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) 14-3-3ε - CG4570 experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - CG6321 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) 14-3-3ε - Chd64 experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Crc experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Df31 experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Eno experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Grp experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Nlp experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Pdi experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Sh3β experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Tango2 experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - Tsc1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) 14-3-3ε - awd experimental knowledge based (Guruharsha et al., 2011) 14-3-3ε - nesd anti tag coimmunoprecipitation, peptide massfingerprinting (Montembault et al., 2010) 14-3-3ε - regucalcin experimental knowledge based (Guruharsha et al., 2011)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions 14-3-3ε allele Gene References 14-3-3ε18A2 phl (Dickson et al., 1996, Chang and Rubin, 1997) phl::tor (Dickson et al., 1996) 14-3-3εj2B10 14-3-3ζ (Chang and Rubin, 1997, Benton et al., 2002, Benton and St. Johnston, 2003) Egfr (Chen and Chien, 1999) par-1 (Benton et al., 2002) phl (Chang and Rubin, 1997) Ras85D (Chang and Rubin, 1997) 14-3-3εS-696 aop (Karim et al., 1996) Dsor1 (Karim et al., 1996) phl (Karim et al., 1996, Chang and Rubin, 1997) phl::tor (Karim et al., 1996, Chang and Rubin, 1997) Ras85D (Karim et al., 1996, Chang and Rubin, 1997) 14-3-3εS-1259 14-3-3ζ (Benton et al., 2002) aop (Karim et al., 1996) Dsor1 (Karim et al., 1996) phl (Karim et al., 1996, Chang and Rubin, 1997) phl::tor (Karim et al., 1996) Ras85D (Karim et al., 1996, Chang and Rubin, 1997) 14-3-3εunspecified phl (Therrien et al., 2000) Ras85D (Therrien et al., 2000) 14-3-3εΔ24 phl (Li et al., 2000) tor (Li and Li, 2003) unspecified ksr (Therrien et al., 2000)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 67207 DroID - A comprehensive database of gene and protein interactions. • FBgn0020238 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42186 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P92177
Orthologs
OrthoDB Orthologs (77) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG63N7ZX Dipteran inclusive ortholog search EOG63N7NN Insect inclusive ortholog search EOG6SN0JP Arthropod inclusive ortholog search EOG6D51DN Metazoa inclusive ortholog search EOG6ZH26P
Orthologs in Drosophila Species (EOG63N7ZX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\14-3-3ε     Drosophila simulans   Dsim\GD15169 Y   Drosophila sechellia   Dsec\GM15292 Y   Drosophila erecta   Dere\GG22781 Y   Drosophila yakuba   Dyak\GE25525 Y   Drosophila ananassae   Dana\GF23073 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA16084 Y   Drosophila persimilis   Dper\GL21790 Y   Drosophila willistoni   Dwil\GK22388 Y   Drosophila virilis   Dvir\GJ22546 Y   Drosophila mojavensis   Dmoj\GI24759 Y   Drosophila grimshawi   Dgri\GH18560 Y
Orthologs in non-Drosophila Dipterans (EOG63N7NN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011116   Anopheles gambiae Malaria mosquito  Agam\AGAP001151   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ013385
Orthologs in non-Dipteran Insects (EOG6SN0JP)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\14-3-3epsilon   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG018274   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11619   Atta cephalotes Leafcutter ant  Acep\ACEP13201   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17224   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15829   Linepithema humile Argentine ant  Lhum\LH25114   Pogonomyrmex barbatus Red harvester ant  Pbar\PB14697   Solenopsis invicta Red fire ant  Sinv\SINV17748   Acyrthosiphon pisum Pea aphid  Apim\ACYPI002517   Bombyx mori Silkmoth  Bmor\BGIBMGA013201   Pediculus humanus Human body louse  Phum\PHUM316950   Tribolium castaneum Red flour beetle  Tcas\TC006109
Orthologs in non-Insect Arthropods (EOG6D51DN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_326737   Ixodes scapularis Deer tick  Isca\ISCW011376
Orthologs in non-Arthropod Metazoa (EOG6ZH26P)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ftt-2 Y Caenorhabditis elegans Nematode  Cele\par-5 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014594gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003825gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_022537gn Y Danio rerio Zebrafish  Drer\ywhaqb Y Danio rerio Zebrafish  Drer\ywhabl Y Danio rerio Zebrafish  Drer\ywhabb Y Danio rerio Zebrafish  Drer\ywhai Y Danio rerio Zebrafish  Drer\ywhaqa Y Danio rerio Zebrafish  Drer\ywhae1 Y Danio rerio Zebrafish  Drer\ywhag2 Y Danio rerio Zebrafish  Drer\ywhag1 Y Danio rerio Zebrafish  Drer\ywhaba Y Danio rerio Zebrafish  Drer\ywhah Y Danio rerio Zebrafish  Drer\ywhae2 Y Xenopus tropicalis Western clawed frog  Xtro\ywhag Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000022830 Y Xenopus tropicalis Western clawed frog  Xtro\ywhah Y Xenopus tropicalis Western clawed frog  Xtro\ywhae Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000026459 Y Xenopus tropicalis Western clawed frog  Xtro\ywhaq Y Gallus gallus Domestic chicken  Ggal\YWHAE Y Gallus gallus Domestic chicken  Ggal\YWHAG Y Gallus gallus Domestic chicken  Ggal\YWHAZ Y Gallus gallus Domestic chicken  Ggal\YWHAB Y Gallus gallus Domestic chicken  Ggal\YWHAH Y Gallus gallus Domestic chicken  Ggal\YWHAQ Y Mus musculus House mouse  Mmus\Ywhae Y Mus musculus House mouse  Mmus\Ywhaz Y Mus musculus House mouse  Mmus\Ywhag Y Mus musculus House mouse  Mmus\Ywhaq Y Mus musculus House mouse  Mmus\Sfn Y Rattus norvegicus Norway rat  Rnor\LOC298795 Y Rattus norvegicus Norway rat  Rnor\Ywhaq Y Rattus norvegicus Norway rat  Rnor\Ywhag Y Rattus norvegicus Norway rat  Rnor\Ywhah Y Rattus norvegicus Norway rat  Rnor\Ywhaz Y Rattus norvegicus Norway rat  Rnor\Ywhab Y Rattus norvegicus Norway rat  Rnor\Ywhae Y Homo sapiens Human  Hsap\YWHAH Y Homo sapiens Human  Hsap\YWHAB Y Homo sapiens Human  Hsap\SFN Y Homo sapiens Human  Hsap\YWHAZ Y Homo sapiens Human  Hsap\YWHAG Y Homo sapiens Human  Hsap\YWHAQ Y Homo sapiens Human  Hsap\ENSG00000108953 Y
Human Orthologs (7)
Gene OMIM HGNC Hsap\YWHAH     Hsap\YWHAB     Hsap\SFN     Hsap\YWHAZ     Hsap\YWHAG     Hsap\YWHAQ     Hsap\ENSG00000108953
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD15169 Drosophila sechellia Dsec\GM15292 Drosophila erecta Dere\GG22781 Drosophila yakuba Dyak\GE25525 Drosophila ananassae Dana\GF23073 Drosophila pseudoobscura pseudoobscura Dpse\GA16084 Drosophila persimilis Dper\GL21790 Drosophila willistoni Dwil\GK22388 Drosophila virilis Dvir\GJ22546 Drosophila mojavensis Dmoj\GI24759 Drosophila grimshawi Dgri\GH18560
Stocks & Reagents
Stocks Listed in FlyBase ( 18 )
Bloomington	17126 w1118; P{EP}14-3-3εEP3423 31497 y1 v1; P{TRiP.HM04001}attP2 6565 14-3-3εS-696/TM3, P{sevRas1.V12}FK2, Sb1
Harvard	- PBac{PB}14-3-3εc06705 - PBac{RB}14-3-3εe00326 - PBac{RB}14-3-3εe03838 - PBac{WH}14-3-3εf05080 - PBac{PB}c02239a, PBac{PB}14-3-3εc02239b
Kyoto	124446 w1118; P{RS3}14-3-3εUM-8256-3 125416 w1118; P{RS5}14-3-3ε5-HA-1828
VDRC	v108129 P{KK102462}VIE-260B v15884 w1118; P{GD4108}v15884
	More stocks available...
Genomic Clones ( 2 )
	BACR03J17 BACR27G04 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 345 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT09839 IP09655
Other clones	MIP08648
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT10314 AT17031 AT21476 AT22740 AT26789 AT28956 AT28965 GH02929 GH09610 GH20203 GM01759 GM02338 GM03741 GM05360 GM06518 GM09044 GM14816 GM23195 GM24044 GM25678 HL03909 LD03745 LD05683 LD05828 LD06063 LD07475 LD07484 LD08541 LD08636 LD09565 LD10119 LD10124 LD12942 LD14275 LD15188 LD16236 LD20088 LD20104 LD23306 LD25634 LD27892 LD30425 LD33287 LD33883 LD33943 LD34057 LD37510 LD39278 LD39915 LD40820 LD44126 LD44634 LD47128 LP02683 LP05739 LP09682 LP11165 LP11639 RE01473 RE04716 RE04774 RE05943 RE06754 RE11392 RE12601 RE16417 RE19293 RE22488 RE22583 RE24554 RE27571 RE31375 RE32373 RE33377 RE35275 RE35993 RE37091 RE37521 RE38255 RE38890 RE40758 RE41254 RE45104 RE46524 RE47093 RE52717 RE53767 RE56016 RE56432 RE60451 RE60464 RE61009 RE61460 RE65296 RE66976 RE67467 RE67951 RE69806 RE70435 RE71429 RE73842 RE74239 RE74502 RH02304 RH16276 RH21818 RH24931 RH26510 RH28115 RH32824 RH35559 RH38439 RH39272 RH43870 RH48751 RH50067 RH58794 RH70504 SD06334 SD11211 SD11765 SD12333 SD13753 SD14572 SD21258 SD22846 SD25595 SD26807
Other clones	1951 12430 28940 35292 37311 37671 39421 46121 47908 53010 54214 58572 59931 60158 62583 71704 81125 81623 82097 83007 100095 102334 106002 107433 109299 119124 121156 149651 172561 174170 174238 214477.58 522192 522536 525020 528137 533578 542621 542738 557143 562160 569654 571244 AT21339 CK01.21A.H6 CK01.29D.C1 CK01.32C.D4 CK01.49D.E9 CK01.73D.H10 CK01.86B.A12 CK01.98A.G2 CK01.108D.E10 CK01.122B.A12 CK01.169D.G7 Dmel_dig_003374_0584_1764 Dmel_dig_009965_0706_3004 Dmel_dig_015487_0692_3357 Dmel_dig_016487_0629_1449 Dmel_dig_016628_0689_1948 Dmel_neb_004671_0689_1266 Dmel_neb_005614_0589_0620 Dmel_neb_008171_0366_2849 Dmel_neb_009806_0845_0300 Dmel_neb_011881_0890_0307 Dmel_neb_013707_0429_1421 Dmel_neb_015239_0843_0599 EC00886 EC38520 EC38570 EK008008 EK026207 EK041219 EK043544 EK046436 EK058063 EK068234 EK075857 EK093434 EK098051 EK104128 EK106201 EK113334 EK123334 EK137512 EK144701 EK146041 EK152612 EK155815 EK155962 EK165034 EK168414 EK171611 EK175834 EK183908 EK188543 EK216917 EK218736 EK231353 EK255401 EK274145 EK274556 EK278429 EK288801 EN00602 EN01534 EN02325 EN04701 EN04708 EN06350 EN08616 EN09763 EN10960 EN11222 EN12131 EN13831 EN15333 EN16425 FGA008H01 FGC029F06 FGH101C06 FGH105E11 FGH105H02 FGH106G01 FGH108A03 FGH118G03 FGM217E08 FGM312C04 FGM313E12 IP11421 IP11512 IP11521 LD03873 LD08053 LD10580 LD11665 LD15268 LD15414 LD15935 LD16048 LD20103 LD20750 LD23076 LD28745 LD29427 LD31757 RE04993 RE09423 RE11566 RE12379 RE18224 RE18446 RE23558 RE31549 RE31590 RE32571 RE33544 RE35265 RE42473 RE42591 RE43750 RE45706 RE47459 RE53449 RE54392 RE56670 RE58322 RE60091 RE61093 RE63158 RE67360 RE71918 RE74503 RH03569 RH04502 RH14144 RH21538 RH29374 RH34590 RH39390 RH44894 RH47980 RH50996 RH56239 RH56313 RH59157 RH64869 RH65996 RH68494 RH71844 RP004054941 RP004088291 RP004088679 RP004091898 RP004131929 RP004161694 RP004162610 RP004236795 RP004245909 RP004246186 RP004246411 RP004248174 RP004277724 RP004354357 RP004368459 RP004387949 RP004408301 RP004486516 RP004488087 RP004544631 UT01604 UUGC0005 UUGC0192 UUGC0467 UUGC1122
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0020238 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42186
Antibody Information
	polyclonal (Tien et al., 1999)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: 14-3-3ε CG8045 (Gong, 1999.11)
Source for database merge of	Source for merge of: 14-3-3ε anon-WO0172774.141 anon-WO02059370.52 (FlyBase, 1996-)
Additional comments	Source for merge of 14-3-3ε anon-WO0172774.141 anon-WO02059370.52 was sequence comparison (date:051113). (FlyBase, 1996-)
Other Comments
	Expression is enriched in embryonic gonads. (Shigenobu et al., 2006) 14-3-3ε is required to time mitosis in undisturbed post-blastoderm cell cycles and to delay mitosis following irradiation in embryos. (Su et al., 2001) 14-3-3ε has been cloned and characterised. Genetic studies suggest that 14-3-3ε functions in multiple receptor tyrosine kinase pathways, acting downstream or parallel to phl, but upstream of aop and phyl, two nuclear factors involved in Ras85D signalling. (Chang and Rubin, 1997) Identified in a genetic screen for modifiers of the phl::tor12D.sev rough eye mutant phenotype. (Dickson et al., 1996) Identified on the basis of genetic interaction with Ras85DV12.sev. (Karim et al., 1996) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. 14-3-3ε gene expression during oogenesis is not critical to embryonic development, but the gene function may be essential for fertilisation and/or completion of meiosis. (Perrimon et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_169796 NM_169797 NM_169798 NM_169799 RefSeq (Proteins) • NP_732309 NP_732310 NP_732311 NP_732312 DDBJ / EMBL / GenBank • AAC47519 AAC47520 AAF55519 AAN13764 AAN13765 AAN13766 AQ026302 AX260179 AX664478 CZ470936 CZ470942 CZ470943 CZ478211 CZ481589 CZ487692 CZ487693 U84897 U84898 Entrez Gene - A searchable database of RefSeq genes. • 42186 UniProtKB/Swiss-Prot • P92177
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • 14-3-3 protein (IPR000308) 14-3-3 protein, conserved site (IPR023409) 14-3-3 domain (IPR023410)
Linkouts
	BioGRID - A database of protein and genetic interactions • 67207 DroID - A comprehensive database of gene and protein interactions. • FBgn0020238 DRSC - Results from RNAi screens. • FBgn0020238 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020238 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG31196-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0020238 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 143529 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 42186 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/14-3-3epsilon.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 42186 modMine - Data generated by the modENCODE project. • FBgn0020238 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • P92177
Synonyms & Secondary IDs ( 33 )
Reported As
Symbol Synonym	14-3-3 (Bilder, 2004, Huynh and St. Johnston, 2004, Jaramillo et al., 2004, Macara, 2004, Hacker et al., 2003, Rebay, 2002, Raabe, 2000, Baek and Lee, 1999, Rommel and Hafen, 1998, Dong et al., 2007, Margolis and Borg, 2005, Nielsen et al., 2008, Kellner et al., 2012) 14-3-3-e (Johannes and Preiss, 2002) 14-3-3e (Walworth, 2000, Chang, 1997.1.11, Chang, 1997.1.10, Teleman et al., 2005, Kaltenbach et al., 2007, Nielsen et al., 2008) 14-3-3epsilon (Spradling et al., 1999, Giot et al., 2003, Buszczak et al., 2007, Quinones-Coello, 2007, Kallappagoudar et al., 2010, Wang et al., 2008, Fisher et al., 2008, Japanese National Institute of Genetics, 2012.5.21) 14-3-3EPSILON (Kondo and Perrimon, 2011) 14-3-3 epsilon (Shirasaki et al., 2012) 14-3-3ε (Yano et al., 2006, Shigenobu et al., 2006, Stuart et al., 2007, Yang and Terman, 2012, Bharadwaj et al., 2013, Zhai et al., 2012, Kaltenbach et al., 2007, Bejarano et al., 2008, Krahn et al., 2009, Alic et al., 2011, Oh and Irvine, 2008, Temme et al., 2009, Oh and Irvine, 2009, Nielsen et al., 2008, Ren et al., 2010, Messaritou et al., 2010, Zhang et al., 2010, Roignant and Treisman, 2010, Mourikis et al., 2010, Kondo and Perrimon, 2011, Montembault et al., 2010, Ling and Salvaterra, 2011, Przewloka et al., 2011, Franco et al., 2011, Yang et al., 2011, Karam et al., 2010, Puri et al., 2008, Pflieger et al., 2008) 14-3-3-ε (Knowles-Barley et al., 2010) 14-3-3 ε (Nie et al., 2009) 14-3-3ο (Nielsen et al., 2008) 14-3-30ε (Nielsen et al., 2008) 143E_DROME (Storm and Sonnhammer, 2003) 1433ε (Nielsen et al., 2008) anon-WO0172774.141   anon-WO02059370.52   CG31196 (Quinones-Coello, 2007, Keleman et al., 2009.8.5, Hughes et al., 2008, Ni et al., 2008, Montembault et al., 2010, Ling and Salvaterra, 2011, Ni et al., 2011.7.19, Cammarato et al., 2011, Puri et al., 2008) CT24092 (Bayraktaroglu, 2000.4.13) D14-3-3e (Skoulakis and Acevedo, 2003) D14-3-3ε (Skoulakis et al., 2004, Skoulakis and Acevedo, 2002, Acevedo and Skoulakis, 2002, Acevedo and Skoulakis, 2001, Tsigkari et al., 2012, Acevedo et al., 2007, Messaritou et al., 2010) d14-3-3ε (Chen and Chien, 1999, Tien et al., 1999, Branco et al., 2008) EK3-5 (Therrien et al., 2000) eps (Tsigkari et al., 2012) l(3)j2B10   PAR5 (Baas et al., 2004) par-5 (Huynh and St. Johnston, 2004, Pellettieri and Seydoux, 2002) Par-5 (Macara, 2004) PAR-5 (Suzuki and Ohno, 2006, Bejarano et al., 2008) SR3-9 (Karim et al., 1996, ) Su(Raf)3B
Name Synonym	14-3-3&epsilon (Glatter et al., 2011) 14-3-3e (Kaltenbach et al., 2007) 14-3-3epsilon (Cammarato et al., 2011) 14-3-3ε (Suzuki and Ohno, 2006, Kaltenbach et al., 2007, Robinson et al., 2010) Epsilon (Tsigkari et al., 2012) Suppressor of Ras85D 3-9 (Karim et al., 1996) Tyrosine-3-monooxygenase (Ling and Salvaterra, 2011)
Secondary FlyBase IDs
	FBgn0011329 FBgn0016739 FBgn0016743 FBgn0046456 FBgn0051196 FBgn0064146 FBgn0066007
References ( 132 )
Generate a list of	All references relating to this gene ( 132 )
List References by type	Research paper  ( 71 ) Supplementary material  ( 9 ) Review  ( 14 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 11 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 2 ) Stock list  ( 1 ) Curated genome annotation  ( 4 ) Patent  ( 2 ) Protein sequence record  ( 1 )
Recent research papers ( 17 )
	Bharadwaj et al., 2013, Development 140(3): 627--638 Cbl-associated protein regulates assembly and function of two tension-sensing structures in Drosophila. [FBrf0220383] Adamson and Lajeunesse, 2012, ScientificWorldJournal 2012: 347597 A Study of Epstein-Barr Virus BRLF1 Activity in a Drosophila Model System. [FBrf0218446] Fabian et al., 2012, Mol. Ecol. 21(19): 4748--4769 Genome-wide patterns of latitudinal differentiation among populations of Drosophila melanogaster from North America. [FBrf0219505] Kellner et al., 2012, Genome Res. 22(6): 1081--1088 Genome-wide phosphoacetylation of histone H3 at Drosophila enhancers and promoters. [FBrf0218468] Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Shirasaki et al., 2012, Neuron 75(1): 41--57 Network organization of the huntingtin proteomic interactome in Mammalian brain. [FBrf0218881] Tsigkari et al., 2012, PLoS ONE 7(5): e36702 14-3-3ε is required for germ cell migration in Drosophila. [FBrf0218503] Yang and Terman, 2012, Neuron 74(1): 108--121 14-3-3ε couples protein kinase A to semaphorin signaling and silences plexin RasGAP-mediated axonal repulsion. [FBrf0218615] Zhai et al., 2012, PLoS Genet. 8(3): e1002582 Antagonistic regulation of apoptosis and differentiation by the cut transcription factor represents a tumor-suppressing mechanism in Drosophila. [FBrf0217859] Alic et al., 2011, Mol. Syst. Biol. 7: 502 Genome-wide dFOXO targets and topology of the transcriptomic response to stress and insulin signalling. [FBrf0214014] Franco et al., 2011, Mol. Cell. Proteomics 10(5): M110.002188 A Novel Strategy to Isolate Ubiquitin Conjugates Reveals Wide Role for Ubiquitination during Neural Development. [FBrf0213919] Glatter et al., 2011, Mol. Syst. Biol. 7: 547 Modularity and hormone sensitivity of the Drosophila melanogaster insulin receptor/target of rapamycin interaction proteome. [FBrf0216604] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Przewloka et al., 2011, Curr. Biol. 21(5): 399--405 CENP-C Is a Structural Platform for Kinetochore Assembly. [FBrf0213151] Shandala et al., 2011, J. Cell Sci. 124(13): 2165--2174 Drosophila 14-3-3{varepsilon} has a crucial role in anti-microbial peptide secretion and innate immunity. [FBrf0213861] Yang et al., 2011, Nat. Struct. Mol. Biol. 18(2): 159--168 RNA secondary structure in mutually exclusive splicing. [FBrf0214471] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...